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Additional file 15: Table S4. The possible TFO and TTS predicted for PLACT1 and IκBα promoter.

Additional file 7: Figure S5. hnRNPA1 is required for PLACT1-induced PDAC progression.

Additional file 10: Figure S8. PLACT1 forms a positive feedback loop with E2F1.

Additional file 9: Figure S7. PLACT1 forms triplexes with promoter sequences of IκBα and regulates its expression.

Additional file 1: Supplementary material and methods.

Additional file 2: Figure S1. The identification of PLACT1 in PDAC.

Additional file 3: Table S1. Univariate and multivariate analysis of disease-free survival in PDAC patients.

Additional file 13: Table S3. Antibodies of experiments.

Additional file 5: Figure S3. PLACT1 enhances proliferation, migration, and invasion of PDAC cells.

Additional file 12: Table S2. Primer and probes of experiments.

Additional file 6: Figure S4. PLACT1 promotes proliferation, migration, and invasion of PDAC cells independent of KRAS/p53.

Additional file 14: Figure S9. Full uncut original pictures.

Additional file 8: Figure S6. PLACT1 induces activation of the NF-κB signaling pathway in an IκBα-dependent manner.

We explore the cellular uptake process of PEGylated liposomes and bicelles by investigating their membrane wrapping process using large-scale molecular dynamics simulations. We find that due to the mobility of ligands on the liposome/bicelle, the membrane wrapping process of a PEGylated liposome/bicelle can be divided into two stages, whose transition is determined by a critical wrapping fraction fc; it is reached when all the ligands are exhausted and bound to receptors within the cell membrane. Before this critical scenario is approached, the grafted polyethylene glycol (PEG) polymers aggregate together within the membrane–wrapped region of the liposome/bicelle, driven by ligand–receptor binding. For wrapping fractions f > fc, membrane wrapping cannot proceed unless a compressive membrane tension is provided. By systematically varying the membrane tension and PEG molar ratio, we establish phase diagrams about wrapping states for both PEGylated liposomes and bicelles. According to these diagrams, we find that the absolute value of the compressive membrane tension required by a fully wrapped PEGylated bicelle is smaller than that of the PEGylated liposome, indicating that the PEGylated bicelle is easily internalized by cells. Further theoretical analysis reveals that compared to a liposome, the flatter surface at the top of a bicelle makes it energetically more favored beyond the critical wrapping fraction fc. Our simulations confirm that the interplay between ligand mobility and NP geometry can significantly change the understanding about the influence of NP geometry on the membrane wrapping process. It can help us to better understand the cellular uptake process of the PEGylated liposome/bicelle and to improve the design of lipid-like NPs for drug delivery., Nanoscale, 11 (34), ISSN:2040-3364, ISSN:2040-3372