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To study the role of Notch1 signaling pathway in the myocardial protective effects of resveratrol pro-treatment after hepatic ischemia/reperfusion injury.Thirty-six healthy male SD rats were randomly divided into 3 groups:sham control (SC) group, hepatic ischemic/reperfusion (HIR) group, and pro-treatment resveratrol (Res) group,12 rats in each group. After each group with hepatic ischemia 40 min reperfusion 2 h (SC group placing equal time), the left ventricular function including left ventricular pressure (LVP), left ventricular systolic pressure (LVSP) and its derivate (±dp/dt) was measured; the serum activities of creatine kinase isoenzyme (CK-MB) and lactate dehydrogenase (LDH) and tumor necrosis factorα(TNF-α) and interleukin-6(IL-6) were detected. The activity of myocardial su-peroxide dismutase(SOD) and contents of myocardial malonaldehyde (MDA) were determined; Notch intracellular domain (NICD) expressions were detected with Western blot; the mRNA levels of Notch1 and TNF-αwere detected by real-time PCR.Compared with SC group, the left ventricular functions in the HIR group were significantly decreased. With decreasing the expression of NICD and Notchl, the activities of CK-MB, LDH, TNF-αand IL-6 in the serum were evidently increased(The findings indicate that resveratrol has protective effect on myocardial injury during the hepatic ischemia/reperfusion, and its mecha-nisms may be related to anti-inflammation and anti-oxidative stress

To investigate whether the phosphatidyl-inositol-3-kinase/protein kinase B(PI3K/Akt)pathway is involved in anti-arrhythmia of resveratrol on ischemia/reperfusion in rat hearts.Forty male rats of normal ECG were randomly divided into 4 groups (The phosphorylated levels of Akt and myocardial Cx43 were significantly enhanced in Res group as compared with I/R group(Resveratrol could prevent the occurrence of reperfusion arrhythmias by increasing the content and ac-tivity of myocardial Cx43 through the PI3K/Akt signaling pathway.

Multilayered SnO2hollow microspheres (MHS-SnO2) have been successfully synthesized via a solvothermal method by using glycol-water as solvent. The morphology, composition and structure of the product have been characterized by field-emission scanning electron microscopy (FESEM) with an energy dispersive X-ray (EDX) spectroscopy, transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM) with selected area electron diffraction (SAED) and X-ray diffraction (XRD). The FESEM, TEM and HRTEM images indicate that the as-prepared microspheres show multilayered structure and the wall of the hollow microspheres is composed of single crystalline nanoparticles. Study on hydrogen absorption characteristics of MHS-SnO2performed at 373 K shows a good absorption capacity of 0.85 wt.%.

Core-shell Ag@ZnO heterostructures are designed and fabricated by a facile two-step chemical method. The heterostructures are composed of Ag microspheres as the core, on which plenty of small ZnO nanorods grow as the shell. The morphological, structural and Raman properties of the composites were investigated by means of powder X-ray diffraction (XRD), scanning electron microscopy (SEM) and Raman scattering spectra. A strong surface-enhanced Raman scattering (SERS) and Raman red shift of ZnO nanorods were observed in Ag@ZnO core-shell nanocomposites due to the effect of Ag microspheres.

Multilayered nanocrystalline SnO2 hollow microspheres (MHS-SnO2) of a rutile structure with a controllable morphology have been successfully synthesised via a chemically induced self-assembly method by using tin chloride pentahydrate and sucrose as precursors in the glycol–water aided solvothermal synthesis. The morphology, composition, structure and luminescent properties of MHS-SnO2 are characterised by field-emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM) with selected area electron diffraction, X-ray diffraction, Raman spectroscopy and photoluminescence spectroscopy. The FESEM, TEM and HRTEM images indicate that the as-prepared microspheres show a multilayer structure and the walls of the hollow microspheres are composed of single crystalline nanoparticles . The effect of calcinating temperature on the Raman and optical properties of the product is analysed and a self-assembly growth mechanism of the MHS-SnO2 is proposed.

To investigate the effects of ischemic postconditioning (IPTC) on the changes of matrix metalloproteinases-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) protein and mRNA levels in rat heart subjected to ischemia/reperfusion, and explore the mechanism by which IPTC protects myocardial interstitium following ischemic/reperfusion (I/R).Twenty four healthy male SD rats were randomly divided into 3 groups (n = 8): sham control (SC) group, I/R group and IPTC group. The parameters of left ventricular function including left ventricular systolic pressure (LVSP) and its derivate (±dp/dt) were measured; the amount of myocardial collagen contents was determined by hydroxyproline quantification; the plasma activity of creatine kinase (CK) and lactate dehydrogenase (LDH) was detected; the protien levels of MMP-2 and TIMP-2 was measured by Western blot and the mRNA levels of MMP-2 and TIMP-2 was detected by real-time PCR.The myocardial collagen contents, left ventricular function and the protein and mRNA levels of TIMP-2 were significantly decreased in I/R group compared with those of SC group, wherease the activities of CK and LDH in the plasma and the protein and mRNA levels of MMP-2 were significantly enhanced in I/R group when compared to SC group. Compared with I/R group, the myocardial collagen contents, left ventricular function and the protein and mRNA levels of TIMP-2 were increased in IPTC group, the activities of CK and LDH in the plasma and the protein and mRNA level of MMP-2 were decreased in IPTC group.These findings indicate that IPTC has protective effects on myocardial interstitial after the myocardial ischemia/reperfusion injury, and IPTC may exert its cardioprotectve effect via inhibiting MMP-2 and enhancing TIMP-2 expression in cardiac muscle.

To investigate the effect of ischemic postconditioning on the expression of rat myocardium matris metalloproteinase-2 (MMP-2) induced by ischemia/reperfusion (I/R) and relationship between its expression and interstitium and the effect on left ventricular function.Twenty-four rats were randomly divided into 3 groups (n = 8): sham control (SC) group, ischemic/reperfusion (I/R) group and ischemic postconditioning (IPTC) group. The left ventricular peak systolic pressure and its derivate (+/- dp/dt) were calculated; The amount of myocardium collagenous were determined; The vitality of superoxide dismutase (SOD) and content of malondialdehyde (MDA) of plasma were detected; The activity of myocardium MMP-2 was measured by Western blot and RT-PCR.As compared with I/R group, IPTC could lower the expression of MMP-2, ameliorate left ventricular function and increase the content of myocardium collagenous. In the meantime, the vitality of superoxide dismutase (SOD) of plasma were greatly enhanced and the content of malondialdehyde (MDA) of plasma were reduced in IFC group.Protective effect of IPIC on myocardium may be due to reduce free radical, lower expression of MMP-2 and protect myocardial interstitium. MMPs plays an important role in the myocardial protection provided by IPTC.