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Additional file 1. Table S1. Specific details for each crude oil used for reporting the results. Table S2. Information on water level adjustments for the microcosm during weathering experiment. Table S3. PAH and APAH concentrations (µg/mL) for the weathered crude oil samples determined by GC-QToF. Table S4. PAH and APAH concentrations (µg/mL) for the un-weathered crude oil samples determined by GC-QToF. Table S5. PASH and APASH concentrations (µg/mL) for the un-weathered crude oil samples determined by GC-QToF. Fig. S1. Set-up for the weathering experiment (May 31st, 2021). Fig. S2. Chromatograms for Dibenzothiophene and C1-C4 Dibenzothiophene for ANS 2015 weathered extracted at 50 ppm. Fig. S3. Chromatograms for Benzonaphthothiophene and C1-C4 Benzonaphthothiophene for ANS 2015 weathered extracted at 50 ppm. Fig. S4. Chromatograms for Dinaphthothiophene and C1-C4 Dinaphthothiophene for ANS 2015 weathered extracted at 50 ppm. Fig. S5. Chromatograms for Dibenzothiophene and C1-C4 Dibenzothiophene for ANS 2015 un-weathered extracted at 50 ppm. Fig. S6. Chromatograms for Benzonaphthothiophene and C1-C4 Benzonaphthothiophene for ANS 2015 un-weathered extracted at 50 ppm. Fig. S7. Chromatograms for Dinaphthothiophene and C1-C4 Dinaphthothiophene for ANS 2015 un-weathered extracted at 50 ppm.