Search

Pancreatic adenocarcinoma (PAAD) has a poor prognosis and is relatively unresponsive to immunotherapy. Gasdermin C (GSDMC) induces pyroptosis in cancer cells and inflammation in the tumor microenvironment. However, whether GSDMC expression in PAAD is associated with survival or response to immunotherapy remains unknown. GSDMC expression and the relationship between GSDMC and patient survival or immune infiltration in PAAD were examined using data in the The Cancer Genome Atlas (TCGA), Gene Expression Ominbus (GEO), Genotype-Tissue Expression (GTEx) and Cancer Cell Line Encyclopedia (CCLE) databases. The TCGA PAAD cohort could be divided into two distinct risk groups based on the expression of GSDMC-related genes (GRGs). The TIDE algorithm predicted that the low-risk group was more responsive to immune checkpoint blockade therapy than the high-risk group. A novel 15-gene signature was constructed and could predict the prognosis of PAAD. In addition, the 15-gene signature model predicted the infiltration of immune cells and Immune checkpoint blockade (ICB) treatment response. Immunohistochemical staining assessment of patient-derived human tissue microarray (TMA) from 139 cases of local PAAD patients revealed a positive correlation between GSDMC expression and PD-L1 expression but a negative correlation between GSDMC expression and infiltration of low CD8+ T cells. Moreover, the overexpression of GSDMC was related to poor overall survival (OS). This study suggests that GSDMC is a valuable biomarker for predicting PAAD prognosis and predicts the immunotherapy response of PAAD.

Objective To investigate the occupational exposure and its risk fatctors among the medical staff of stomatological department and, in order to improve the occupational protection and reduce the risk for occupational exposure.Methods The occupational exposures that occurred in 44 medical staff from Jan 2013 to Dec 2015 were investigated, and the occupation, exposure sources, types of exposures, exposure link were analyzed.Results Among the 44 medical staff of oral department with occupational exposure, doctors accounted for 54.5%, nurses 34.1%. There were 39 medical staff with sharps injuries and 3 medical staff with mucous membrane exposure. The major expose reason is cleaning medical instruments or equipment at the end of medical treatment, accounting for 43.2%(19 cases). Needle stick injures in operation is the second major reason, accounting for 34.1%(15 cases). All the subjects with occupational exposures have received appropriate treatment, and the follow-up showed that none of the medical staff were infected due to the occupational exposure.Conclusion The major expose reason is cleaning medical instruments or equipment at the end of medical treatment. It is crucial to intensify the training of medical staff of stomatological department in the knowledge of occupational exposure, implement the standard of preventive measures, and standarize the operation procedures so as to reduce the risk of occupational exposure.