1. Biodegradation and decolourization of methyl red by Aspergillus versicolor LH1
- Author
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Liang Zhi Li, Heng Wei Liu, Cui Ying Hu, Xue Mei Yao, Jiao Long Fu, Li Shi Yan, Wei Qiang Guo, and Hong Ying Cheng
- Subjects
0106 biological sciences ,010405 organic chemistry ,General Medicine ,Biodegradation ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Industrial wastewater treatment ,chemistry.chemical_compound ,Activated sludge ,chemistry ,010608 biotechnology ,Methyl red ,Micronucleus test ,Aspergillus versicolor ,Micronucleus ,Biotechnology ,Nuclear chemistry ,Benzoic acid - Abstract
Azo dyes constitute a significant environmental burden due to its toxicity, carcinogenicity, and hard biodegradation. The report here is focused on the decolorization and degradation treatment of azo dye methyl red (MR). Decolorization of MR using Aspergillus versicolor LH1 isolated from activated sludge was investigated. The maximum decolorization rate of 92.3% was obtained under the optimized conditions of sucrose as carbon source, 5d incubation age, pH 6.0, 140 mg/L initial concentration of MR and 2.5 g/L initial concentration of NaNO3. Biodegradation products of MR were investigated using HPLC-MS, FTIR, and GC-MS assays. It was revealed the three bonds of -C-N = in MR aromatic nucleus were disrupted, and benzoic acid was detected. Micronucleus test with Glycine max L. and Vicia faba L. demonstrated that MCN‰ (micronucleus permillage) of MR metabolites was less than MR solution. These findings provided evidence that A. versicolor LH1 is a candidate for MR degradation in industrial wastewater treatment.
- Published
- 2020