60 results on '"Xianbo Zheng"'
Search Results
2. The engineered <scp>CRISPR‐Mb2Cas12a</scp> variant enables sensitive and fast nucleic acid‐based pathogens diagnostics in the field
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Jian Jiao, Yiqi Liu, Mengli Yang, Jingcheng Zheng, Chonghuai Liu, Wenxiu Ye, Shangwei Song, Tuanhui Bai, Chunhui Song, Miaomiao Wang, Jiangli Shi, Ran Wan, Kunxi Zhang, Pengbo Hao, Jiancan Feng, and Xianbo Zheng more...
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Plant Science ,Agronomy and Crop Science ,Biotechnology - Published
- 2023
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3. Peach DELLA Protein PpeDGYLA Is Not Degraded in the Presence of Active GA and Causes Dwarfism When Overexpressed in Poplar and Arabidopsis
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Yun Chen, Mengmeng Zhang, Xiaofei Wang, Yun Shao, Xinyue Hu, Jun Cheng, Xianbo Zheng, Bin Tan, Xia Ye, Wei Wang, Jidong Li, Ming Li, Langlang Zhang, and Jiancan Feng
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Inorganic Chemistry ,Organic Chemistry ,Prunus persica ,DGLLA ,DGYLA ,gibberellins ,dwarfing ,evolutionary relationship ,General Medicine ,Physical and Theoretical Chemistry ,Molecular Biology ,Spectroscopy ,Catalysis ,Computer Science Applications - Abstract
Controlling the tree size of fruit species such as peach can reduce the amount of labor and input needed for orchard management. The phytohormone gibberellin (GA) positively regulates tree size by inducing degradation of the GA signaling repressor DELLA. The N-terminal DELLA domain in this protein is critical for its GA-dependent interaction with the GA receptor GID1 and the resulting degradation of the DELLA protein, which allows for growth-promoting GA signaling. In this study, a DELLA family member, PpeDGYLA, contains a DELLA domain but has amino acid changes in three conserved motifs (DELLA into DGYLA, LEQLE into LERLE, and TVHYNP into AVLYNP). In the absence or presence of GA3, the PpeDGYLA protein did not interact with PpeGID1c and was stable in 35S-PpeDGYLA peach transgenic callus. The overexpression of PpeDGYLA in both polar and Arabidopsis showed an extremely dwarfed phenotype, and these transgenic plants were insensitive to GA3 treatment. PpeDGYLA could interact with PpeARF6-1 and -2, supposed growth-promoting factors. It is suggested that the changes in the DELLA domain of PpeDGYLA may, to some extent, account for the severe dwarf phenotype of poplar and Arabidopsis transgenic plants. In addition, our study showed that the DELLA family contained three clades (DELLA-like, DELLA, and DGLLA). PpeDGYLA clustered into the DGLLA clade and was expressed in all of the analyzed tissues. These results lay the foundation for the further study of the repression of tree size by PpeDGYLA. more...
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- 2023
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4. Multiple indeterminate domain (IDD)–DELLA1 complexes participate in gibberellin feedback regulation in peach
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Yajun Jiang, Jiajia Chen, Xianbo Zheng, Bin Tan, Xia Ye, Wei Wang, Langlang Zhang, Jidong Li, Zhiqian Li, Jun Cheng, and Jiancan Feng
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Prunus persica ,Arabidopsis Proteins ,Gene Expression Regulation, Plant ,Arabidopsis ,Genetics ,Plant Science ,General Medicine ,Agronomy and Crop Science ,Gibberellins ,Feedback ,Plant Proteins - Abstract
Peach encodes 14 INDETERMINATE DOMAIN (IDD) transcription factors. PpIDD4, -12 and -13 mediated PpDELLA1 binding to the PpGA20ox1 promoter. Each of these three PpIDD-DELLA1 complexes activated transcription of PpGA20ox1. PpTPR1 and -4 interrupted the interaction of PpIDDs with PpDELLA1. The plant growth regulator gibberellin (GA) plays an important role in the rapid growth of annual shoots in peach. Our previous study showed that the peach cultivar 'FenHuaShouXingTao' (FHSXT), a gibberellic acid receptor (gid1) mutant, accumulates active GAs in annual shoot tips. This mutant enhances GA feedback regulation in peach. The results of this study suggested that the PpIDD-DELLA1 complex is the underlying mechanism of GA feedback regulation in peach. Fourteen IDD genes were identified in peach, and three PpIDDs (PpIDD4, -12 and -13, all from group IV) interacted with PpDELLA1, an important component in GA signaling pathway. Truncation, segmentation and site mutation of the promoter of PpGA20ox1 (a GA biosynthesis gene) showed that all three PpIDD proteins recognized the core motif TTGTC. PpIDD4 and -13 mainly bind to site 3, while PpIDD12 binds to site 5 of the PpGA20ox1 promoter. All three PpIDD-DELLA1 complexes activated the PpGA20ox1 promoter-LUC fusion. These data suggested that PpIDDs bridge PpDELLA1 and the promoter of PpGA20ox1, which then activated the transcription of PpGA20ox1. In addition, PpTPR1 and -4 disrupted the interaction of PpIDDs with PpDELLA1. Our research will be helpful for understanding and possibly modifying the regulation of annual shoot growth and GA biosynthesis. more...
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- 2022
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5. De novo chromosome‐level genome of a semi‐dwarf cultivar of Prunus persica identifies the aquaporin PpTIP2 as responsible for temperature‐sensitive semi‐dwarf trait and PpB3‐1 for flower type and size
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Xiaodong Lian, Haipeng Zhang, Chao Jiang, Fan Gao, Liu Yan, Xianbo Zheng, Jun Cheng, Wei Wang, Xiaobei Wang, Xia Ye, Jidong Li, Langlang Zhang, Zhiqian Li, Bin Tan, and Jiancan Feng
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Plant Science ,Agronomy and Crop Science ,Biotechnology - Published
- 2022
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6. Overexpression of peach NAC25 promotes anthocyanin biosynthesis in poplar shoots
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Mingxi Geng, Yun Shao, Mengmeng Zhang, Xianbo Zheng, Bin Tan, Wei Wang, Langlang Zhang, Xia Ye, Ming Li, Jidong Li, Jun Cheng, and Jiancan Feng
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- 2022
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7. VvPL11 Is a Key Member of the Pectin Lyase Gene Family Involved in Grape Softening
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Wenxin Li, Chang He, Hongli Wei, Jiakang Qian, Jiannan Xie, Zhiqian Li, Xianbo Zheng, Bin Tan, Jidong Li, Jun Cheng, Wei Wang, Xia Ye, and Jiancan Feng
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propectin ,pectin lyase ,Plant Science ,Horticulture ,grape berry ,Vitis labrusca × Vitis vinifera ,ripening - Abstract
Fruit ripening includes several metabolic changes that lead to sweeter and softer fruit. Pectin depolymerization is one of major factors that softens developing grape berries. Pectin lyases (PLs) play important roles in pectin degradation in the grape berry. However, little is known about the temporal and spatial expression of grapevine (Vitis spp.) pectin lyase genes (VvPLs) or their function during fruit ripening and softening. In this study, 18 individual VvPL genes were identified in the grape genome. All VvPL genes were sorted into group I and group II, except VvPL12 which demonstrated higher and similar expression trends in different tissues and organs. In grape berry, VvPL1, 5, 7, 11 and 16 were highly expressed, whereas VvPL18, 15, 2, 13, 10, 14, 17, 6 and 8 showed lower expression levels at different berry developmental stages. Expression of VvPL11 firstly increased and then decreased, and the highest expression was shown at 6 weeks after full bloom (WAFB) during berry development. Over-expression of the VvPL11 gene in tomato caused higher ethylene production and lower firmness compared to wild-type fruit. Moreover, decreased propectin and increased water-soluble pectin (WSP) levels were observed in VvPL11 transgenic tomato fruit. Consistent with this result, the expression levels of SlPG2, SlEXP, and SlPME1, all of which are genes involved in fruit softening, were up-regulated in VvPL11-OE tomato fruit, which supported the idea that VvPL11 plays an important role in fruit ripening and softening. This study provided a comprehensive analysis of the grapevine PL family and advanced our knowledge of the functions of VvPLs during fruit softening. more...
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- 2023
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8. Genome-wide identification of LBD transcription factors in apple and the function of MdLBD16a in adventitious rooting and callus development
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Ruirui Wang, Tuanhui Bai, Haoyuan Gao, Yajun Cui, Ruli Zhou, Zhengyang Wang, Shangwei Song, Jian Jiao, Miaomiao Wang, Ran Wan, Jiangli Shi, Pengbo Hao, Kunxi Zhang, Jiancan Feng, Chunhui Song, and Xianbo Zheng more...
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Horticulture - Published
- 2023
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9. Phylogenetic and Transcriptional Analyses of the HSP20 Gene Family in Peach Revealed That PpHSP20-32 Is Involved in Plant Height and Heat Tolerance
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Xiaodong Lian, Qiuping Wang, Tianhao Li, Hongzhu Gao, Huannan Li, Xianbo Zheng, Xiaobei Wang, Haipeng Zhang, Jun Cheng, Wei Wang, Xia Ye, Jidong Li, Bin Tan, and Jiancan Feng
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Inorganic Chemistry ,Organic Chemistry ,peach (Prunus persica) ,HSP20 ,functional identification ,PpHSP20-32 ,thermotolerance ,General Medicine ,Physical and Theoretical Chemistry ,Molecular Biology ,Spectroscopy ,Catalysis ,Computer Science Applications - Abstract
The heat shock protein 20 (HSP20) proteins comprise an ancient, diverse, and crucial family of proteins that exists in all organisms. As a family, the HSP20s play an obvious role in thermotolerance, but little is known about their molecular functions in addition to heat acclimation. In this study, 42 PpHSP20 genes were detected in the peach genome and were randomly distributed onto the eight chromosomes. The primary modes of gene duplication of the PpHSP20s were dispersed gene duplication (DSD) and tandem duplication (TD). PpHSP20s in the same class shared similar motifs. Based on phylogenetic analysis of HSP20s in peach, Arabidopsis thaliana, Glycine max, and Oryza sativa, the PpHSP20s were classified into 11 subclasses, except for two unclassified PpHSP20s. cis-elements related to stress and hormone responses were detected in the promoter regions of most PpHSP20s. Gene expression analysis of 42 PpHSP20 genes revealed that the expression pattern of PpHSP20-32 was highly consistent with shoot length changes in the cultivar ‘Zhongyoutao 14’, which is a temperature-sensitive semi-dwarf. PpHSP20-32 was selected for further functional analysis. The plant heights of three transgenic Arabidopsis lines overexpressing PpHSP20-32 were significantly higher than WT, although there was no significant difference in the number of nodes. In addition, the seeds of three over-expressing lines of PpHSP20-32 treated with high temperature showed enhanced thermotolerance. These results provide a foundation for the functional characterization of PpHSP20 genes and their potential use in the growth and development of peach. more...
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- 2022
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10. Phylogenetic and Transcriptional Analyses of the
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Xiaodong, Lian, Qiuping, Wang, Tianhao, Li, Hongzhu, Gao, Huannan, Li, Xianbo, Zheng, Xiaobei, Wang, Haipeng, Zhang, Jun, Cheng, Wei, Wang, Xia, Ye, Jidong, Li, Bin, Tan, and Jiancan, Feng
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Prunus persica ,Thermotolerance ,Gene Expression Regulation, Plant ,Multigene Family ,Arabidopsis ,Plants ,Genome, Plant ,Heat-Shock Proteins ,Hormones ,Phylogeny ,Plant Proteins - Abstract
The heat shock protein 20 (HSP20) proteins comprise an ancient, diverse, and crucial family of proteins that exists in all organisms. As a family, the HSP20s play an obvious role in thermotolerance, but little is known about their molecular functions in addition to heat acclimation. In this study, 42 more...
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- 2022
11. Establishment of an efficient micropropagation system in enhancing rooting efficiency via stem cuttings of apple rootstock M9T337
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Jian Jiao, Xianbo Zheng, Jiangli Shi, Chunhui Song, Miaomiao Wang, Tuanhui Bai, Zhidan Dong, Shangwei Song, and Xie Beiyang
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Horticulture ,Cutting ,Micropropagation ,Biology ,Rootstock - Published
- 2021
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12. Genome-wide identification and expression of the lipoxygenase gene family in jujube (Ziziphus jujuba) in response to phytoplasma infection
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Qicheng Li, Jidong Li, Jiancan Feng, Yu Zhang, L. L. Chen, Bin Tan, Chen Peng, Qiqi Yang, Xia Ye, and Xianbo Zheng
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0106 biological sciences ,0301 basic medicine ,Genetics ,Phylogenetic tree ,Plant Science ,Biology ,biology.organism_classification ,01 natural sciences ,Genome ,food.food ,03 medical and health sciences ,030104 developmental biology ,food ,Ziziphus jujuba ,Phytoplasma ,Gene duplication ,Plant defense against herbivory ,Gene family ,Agronomy and Crop Science ,Gene ,010606 plant biology & botany ,Biotechnology - Abstract
Chinese jujube (Ziziphus jujuba) is an important fruit tree in China. Jujube witches' broom (JWB) disease, caused by JWB phytoplasma (‘Candidatus phytoplasma ziziphi’), is the most devastating infectious disease of the jujube tree. Lipoxygenase (LOX) plays important roles in the jasmonic acid (JA) biosynthesis pathway and plant defense to biotic stresses. In this study, we identified 13 LOX genes in the jujube genome distributed on 5 chromosomes. The ZjLOX proteins were classified into two categories based on phylogenetic analysis. We tracked gene duplication events and found two sets of tandem repeats on chromosome 2. We predicted the promoters of the ZjLOXs using PlantCARE and found a large number of cis elements that may respond to phytohormones, light, and biological stresses. Transcriptomic, proteomic, and qRT-PCR expression analyses showed that ZjLOX02, ZjLOX05, ZjLOX06 and ZjLOX08 may play important roles in the interaction between jujube and phytoplasma. This study provides a foundation for better understanding both the molecular evolution of the jujube LOX gene family and the response of the ZjLOX genes to JWB phytoplasma infection. more...
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- 2021
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13. Comparative Physiological and Transcriptome Analysis Reveals Potential Pathways and Specific Genes Involved in Waterlogging Tolerance in Apple Rootstocks
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Kunxi Zhang, Xiaofei Chen, Penghao Yuan, Chunhui Song, Shangwei Song, Jian Jiao, Miaomiao Wang, Pengbo Hao, Xianbo Zheng, and Tuanhui Bai
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Inorganic Chemistry ,waterlogging stress ,apple rootstocks ,flavonoids ,RNA-seq ,ethylene ,Organic Chemistry ,General Medicine ,Physical and Theoretical Chemistry ,Molecular Biology ,Spectroscopy ,Catalysis ,Computer Science Applications - Abstract
Apple (Malus × domestica Borkh.) is one of the most cultivated fruit crops in China. Apple trees frequently encounter waterlogging stress, mainly due to excess rainfall, soil compaction, or poor soil drainage, results in yellowing leaves and declined fruit quality and yield in some regions. However, the mechanism underlying the response to waterlogging has not been well elucidated. Therefore, we performed a physiological and transcriptomic analysis to examine the differential responses of two apple rootstocks (waterlogging-tolerant M. hupehensis and waterlogging-sensitive M. toringoides) to waterlogging stress. The results showed that M. toringoides displayed more severe leaf chlorosis during the waterlogging treatment than M. hupehensis. Compared with M. hupehensis, the more severe leaf chlorosis induced by waterlogging stress in M. toringoides was highly correlated with increased electrolyte leakage and superoxide radicals, hydrogen peroxide accumulation, and increased stomata closure. Interestingly, M. toringoides also conveyed a higher ethylene production under waterlogging stress. Furthermore, RNA-seq revealed that a total of 13,913 common differentially expressed genes (DEGs) were differentially regulated between M. hupehensis and M. toringoides under waterlogging stress, especially those DEGs involved in the biosynthesis of flavonoids and hormone signaling. This suggests a possible link of flavonoids and hormone signaling to waterlogging tolerance. Taken together, our data provide the targeted genes for further investigation of the functions, as well as for future molecular breeding of waterlogging-tolerant apple rootstocks. more...
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- 2023
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14. Genome Wide Transcriptomic Analysis of WRKY Gene Family Response to Biotic Stresses in Malus ×domestica
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Tuanhui Bai, Hanghang Zhang, Ruli Zhou, Liu Yang, Miaomiao Wang, Jian Jiao, Xianbo Zheng, Shangwei Song, Yingli Zhang, Chunhui Song, Zhengyang Wang, Hongguang Pang, and Feng Jiancan
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Gymnosporangium yamadae ,Genetics ,Malus ,biology ,fungi ,food and beverages ,Botryosphaeria dothidea ,General Medicine ,biology.organism_classification ,Alternaria alternata ,Genome ,WRKY protein domain ,Pythium ultimum ,bacteria ,Gene - Abstract
Apple (Malus × domestica Borkh.) is a perennial woody plant that often suffers from various biological stresses. Many harmful pathogens can infect apple trees and lead to reduced production. We comprehensively identified the WRKY genes in the apple genome and analyzed their expression in response to several biological stressors, including Alternaria alternata, Pythium ultimum, Botryosphaeria dothidea, Erwinia amylovora, Penicillium expansum, Gymnosporangium yamadae, and Apple replant disease. There were 113 MdWRKYs identified in the apple genome. Twenty-two MdWRKYs were differentially expressed in response to at least five pathogens. Promoter sequence analysis showed that these genes carried many defense- and stress-responsive elements, such as MeJA-response elements, salicylic acid-response elements, and W-box elements, in their promoters. Transient expression assays showed that MdWRKY40a and MdWRKY54h played negative roles in defense against B. dothidea infection. WRKY40 and WRKY60 and the MdWKRY33s might play important roles in responding to pathogens and are conserved in some plants. These differentially expressed MdWRKYs might play key roles in the apple response to multiple pathogens. more...
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- 2021
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15. Rapid detection of multiple phytoplasma with an All-In-One Dual (AIOD) CRISPR assay
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Peng Chen, Qicheng Li, Jian Jiao, Qiqi Yang, Sicheng Guo, Jia Yao, yun Chen, Xia Ye, Jun Cheng, Bin Tan, Xianbo Zheng, Yingxia Wu, Hong Cai, Yunfeng Wu, Huiyu Wang, Jidong Li, and Jiancan Feng
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Phytoplasma can infect thousands of plants and caused huge economic losses around the world. The large-scale spread and serious lethality of phytoplasma prompt the urgent need for sensitive, accurate, visual and rapid detection of these pathogens. Current molecular assays used for detecting phytoplasma are expensive and time consuming. Here, we established a novel All-In-One Dual (AIOD) CRISPR detection platform based on CRISPR/LbCas12a technology and Recombinase Polymerase Amplification (RPA) for the diagnosis of multiple phytoplasma. The protocol is simple, requiring one vessel, rapid and sensitive, and the output is visual. Cas12a/crRNAs complexes are added into a reaction containing RPA Mix, RPA Primers and single-stranded DNA fluorophore-quencher (ssDNA-FQ). All components, including 1 μL of sample DNA, are added together and then incubated in one tube at 37 °C. Phytoplasma was detected after 15 min or less from leaf harvest. Positive results can be observed by the naked eye via fluorescent signals. We optimized the amounts of crRNA, LbCas12a and the ssDNA fluorophore in the detection system. Finally, an optimized system was established containing 1,000 nM ssDNA-FQ and a 2:1:1 ratio of LbCas12a/crRNA1/crRNA2 complex with a 0.8 μM concentration as 1. In the optimized reaction, the AIOD-CRISPR detection system exhibited high sensitivity, with limits of detection reaching 3.37E + 2 copies of phytoplasma DNA per reaction. Field tests indicated the AIOD-CRISPR detection system possessed high specificity and reached the 100% accuracy when compared with PCR detection. In conclusion, the AIOD-CRISPR detection system is a ideal selection with high specificity and sensitivity for phytoplasma detection. Our work provides a technique that can be potentially used to rapidly and simultaneously detect more pathogens. more...
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- 2022
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16. The Jujube TCP Transcription Factor ZjTCP16 Regulates Plant Growth and Cell Size by Affecting the Expression of Genes Involved in Plant Morphogenesis
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Qiqi Yang, Qicheng Li, Liyuan Gu, Peng Chen, Yu Zhang, Yonghua Li, Yun Chen, Xia Ye, Bin Tan, Xianbo Zheng, Jidong Li, and Jiancan Feng
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TCP transcription factor ,gene interaction ,dwarf ,small leaf ,morphogenesis ,Forestry - Abstract
Jujube production is threatened by jujube witches’ broom (JWB) disease, which is caused by JWB phytoplasma. The jujube TCP transcription factor (TF) ZjTCP16 may be involved in the interaction of jujube plants with JWB phytoplasma. In this study, qRT-PCR proved that the expression pattern of ZjTCP16 was altered by JWB phytoplasma. The gene functions of ZjTCP16 were analyzed by its overexpression in Arabidopsis and jujube, as well as knock-down in. The overexpression of ZjTCP16 in Arabidopsis and jujube resulted in dwarfism and small leaves, while the zjtcp16 CRISPR mutants were higher than the WT. Microscopic observation of paraffin sections of jujube stems showed that ZjTCP16 affected the size of cells. The interactions of ZjTCP16 with ZjAS2 and ZjLOB in both the cytoplasm and nucleus were demonstrated by yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. Yeast one-hybrid (Y1H) assays and qRT-PCR further confirmed that ZjTCP16 affected the expression of genes involved in leaf morphogenesis and cell proliferation (ZjAS1, ZjKNAT1, ZjKNAT2 and ZjKNAT6) at the mRNA level through the ZjAS2 and ZjLOB pathways. In conclusion, ZjTCP16 regulates plant growth and cell size by altering the expression pattern of morphogenesis-related genes in jujube. more...
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- 2022
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17. Field detection of multiple RNA viruses/viroids in apple using a CRISPR/Cas12a‐based visual assay
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Xianbo Zheng, Miaomiao Wang, Chunhui Song, Zhenli Yan, Jian Jiao, Han Jinmeng, Jiancan Feng, Shangwei Song, Kong Kangkang, Ruiping Zhang, Hengtao Zhang, and Tuanhui Bai
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0106 biological sciences ,0301 basic medicine ,Viroid ,viruses ,RT‐RPA ,Metal Nanoparticles ,in‐field application ,Plant Science ,Sensitivity and Specificity ,complex mixtures ,01 natural sciences ,Virus ,apple viral diseases ,03 medical and health sciences ,RNA Viruses ,CRISPR ,Clustered Regularly Interspaced Short Palindromic Repeats ,Multiplex ,Research Articles ,Plant Diseases ,biology ,fungi ,LbCas12a ,RNA ,RNA virus ,biology.organism_classification ,Virology ,Viroids ,Apple stem pitting virus ,030104 developmental biology ,RNA, Viral ,Gold ,CRISPR-Cas Systems ,visualized detection ,Agronomy and Crop Science ,Apple stem grooving virus ,Research Article ,010606 plant biology & botany ,Biotechnology - Abstract
Summary Co‐infection of apple trees with several viruses/viroids is common and decreases fruit yield and quality. Accurate and rapid detection of these viral pathogens helps to reduce losses and prevent virus spread. Current molecular detection assays used for apple viruses require specialized and expensive equipment. Here, we optimized a CRISPR/Cas12a‐based nucleic acid detection platform for the diagnosis of the most prevalent RNA viruses/viroid in apple, namely Apple necrotic mosaic virus (ApNMV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV) and Apple scar skin viroid (ASSVd). We detected each RNA virus/viroid directly from crude leaf extracts after simultaneous multiplex reverse transcription‐recombinase polymerase amplification (RT‐RPA) with high specificity. Positive results can be distinguished by the naked eye via oligonucleotide‐conjugated gold nanoparticles. The CRISPR/Cas12a‐RT‐RPA platform exhibited comparable sensitivity to RT‐qPCR, with limits of detection reaching 250 viral copies per reaction for ASPV and ASGV and 2500 copies for the others. However, this protocol was faster and simpler, requiring an hour or less from leaf harvest. Field tests showed 100% agreement with RT‐PCR detection for 52 samples. This novel Cas12a‐based method is ideal for rapid and reliable detection of apple viruses in the orchard without the need to send samples to a specialized laboratory. more...
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- 2020
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18. The distribution of bioactive gibberellins along peach annual shoots is closely associated with PpGA20ox and PpGA2ox expression profiles
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Mengmeng Zhang, Yangtao Ma, Xianbo Zheng, Bin Tan, Xia Ye, Wei Wang, Langlang Zhang, Jidong Li, Zhiqian Li, Jun Cheng, and Jiancan Feng
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Prunus persica ,Gene Expression Regulation, Plant ,Genetics ,Arabidopsis ,Gibberellins ,Biotechnology ,Plant Proteins - Abstract
Background The rapid growth of annual shoots is detrimental to peach production. While gibberellin (GA) promotes the rapid growth of peach shoots, there is limited information on the identity and expression profiles of GA-metabolism genes for this species. Results All six GA biosynthetic gene families were identified in the peach genome, and the expression profiles of these family members were determined in peach shoots. The upstream biosynthetic gene families have only one or two members (1 CPS, 2 KSs, and 1 KO), while the downstream gene families have multiple members (7 KAOs, 6 GA20oxs, and 5 GA3oxs). Between the two KS genes, PpKS1 showed a relatively high transcript level in shoots, while PpKS2 was undetectable. Among the seven KAO genes, PpKAO2 was highly expressed in shoots, while PpKAO1 and − 6 were weakly expressed. For the six GA20ox genes, both PpGA20ox1 and − 2 were expressed in shoots, but PpGA20ox1 levels were higher than PpGA20ox2. For the five GA3ox genes, only PpGA3ox1 was highly expressed in shoots. Among these biosynthesis genes, PpGA20ox1 and PpGA3ox1 showed a gradual decrease in transcript level along shoots from top to bottom, and a similar trend was observed in bioactive GA1 and GA4 distribution. Among the GA-deactivation genes, PpGA2ox6 was highly expressed in peach shoots. PpGA2ox1 and − 5 transcripts were relatively lower and showed a similar pattern to PpGA20ox1 and PpGA3ox1 in peach shoots. Overexpression of PpGA20ox1, − 2, or PpGA2ox6 in Arabidopsis or tobacco promoted or depressed the plant growth, respectively, while PpGA3ox1 did not affect plant height. Transient expression of PpGA20ox1 in peach leaves significantly increased bioactive GA1 content. Conclusions Our results suggest that PpGA20ox and PpGA2ox expression are closely associated with the distribution of active GA1 and GA4 in peach annual shoots. Our research lays a foundation for future studies into ways to effectively repress the rapid growth of peach shoot. more...
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- 2022
19. Phytoplasma effector Zaofeng6 induces shoot proliferation by decreasing the expression of ZjTCP7 in Ziziphus jujuba
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Peng Chen, Lichuan Chen, Xia Ye, Bin Tan, Xianbo Zheng, Jun Cheng, Wei Wang, Qiqi Yang, Yu Zhang, Jidong Li, and Jiancan Feng
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Genetics ,Plant Science ,Horticulture ,Biochemistry ,Article ,Biotechnology - Abstract
The jujube witches’ broom (JWB) phytoplasma is associated with witches’ broom, dwarfism, and smaller leaves in jujube, resulting in yield losses. In this study, eight putative JWB effector proteins were identified from potential mobile units of the JWB genome. Among them, Zaofeng6 induced witches’ broom symptoms in Arabidopsis and jujube. Zaofeng6-overexpressing Arabidopsis and unrooted jujube transformants displayed witches’ broom-like shoot proliferation. Transient expression of Zaofeng6 induced hypersensitive response like cell death and expression of hypersensitive response marker genes, like harpin-induced gene 1 (H1N1), and the pathogenesis-related genes PR1, PR2, and PR3 in transformed Nicotiana benthamiana leaves, suggesting that Zaofeng6 could be a virulence effector. Yeast two-hybrid library screening and bimolecular fluorescence complementation confirmed that Zaofeng6 interacts with ZjTCP7 through its first two α-helix domains in the cell nuclei. ZjTCP7 mRNA and protein abundance decreased in Zaofeng6 transgenic jujube seedlings. The expression of some genes in the strigolactone signaling pathway (ZjCCD7, ZjCCD8, and CYP711A1) were down-regulated in jujube shoots overexpressing Zaofeng6 and in zjtcp7 CRISPR/Cas9 mutants. Zaofeng6 induces shoot proliferation through decreased expression of ZjTCP7 at the transcriptional and translational levels. more...
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- 2022
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20. Pppif8, a Della2-Interacting Protein, Regulates Peach Shoot Elongation Possibly Through Auxin Signaling
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Yun Chen, Mengmeng Zhang, Yingcong Wang, Xianbo Zheng, Haipeng Zhang, Langlang Zhang, Bin Tan, Xia Ye, Wei Wang, Jidong Li, Ming Li, Jun Cheng, and Jiancan Feng
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History ,Indoleacetic Acids ,Light ,Polymers and Plastics ,Arabidopsis Proteins ,Arabidopsis ,Plant Science ,General Medicine ,Hypocotyl ,Industrial and Manufacturing Engineering ,Gene Expression Regulation, Plant ,Genetics ,Business and International Management ,Agronomy and Crop Science - Abstract
Rapid growth of branches in a peach tree restricts the light penetration and air ventilation within the orchard, which lowers fruit quality and promotes the occurrence of diseases and insects. Our previous works showed that PpDELLA1 and PpDELLA2 repress the rapid growth of annual shoots. Proteins that interact with DELLA are vital for its function. In this study, seven PpPIFs (PpPIF1, -2, -3, -4, -6, -7 and -8) were identified in the peach genome and contain a conserved bHLH domain. Among the seven PpPIFs, PpPIF8 interacted with PpDELLA2 through an unknown motif in the C-terminal and/or the bHLH domain. Overexpression of PpPIF8 in Arabidopsis promotes plant height and branch numbers. Hypocotyl elongation was significantly enhanced by PpPIF8 under weak light intensity. PpPIF8 overexpressed in Arabidopsis and transiently expressed in peach seedlings upregulated the transcription of YUCCA and SAUR19 and downregulated SHY1 and -2. Additionally, PpPIF4 and -8 were significantly induced by weak light. Phylogentic analysis and intron patterns of the bHLH domain strongly suggested that PIFs from six species could be divided into two groups of different evolutionary origins. These results lay a foundation for the further study of the repression of shoot growth by PpDELLA2 through protein interaction with PpPIF8 in peach. more...
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- 2022
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21. Ppibh1-1 Limits Internode Elongation of Peach Shoot in a Dose-Dependent Manner
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Mengmeng Zhang, Beibei Feng, Yun Chen, Mingxi Geng, Ming Li, Xianbo Zheng, Haipeng Zhang, Langlang Zhang, Bin Tan, Xia Ye, Wei Wang, Jidong Li, Jun Cheng, and Jiancan Feng
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Genetics ,Plant Science ,General Medicine ,Agronomy and Crop Science - Published
- 2022
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22. VvERF111 Regulates Chlorophyll Degradation by Activating Expression of VvCLH1, Leading to Rachis Browning in Grape
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Dongfang Zou, Jingwen Li, Xia Ye, Xianbo Zheng, Bin Tan, Jun Cheng, Wei Wang, Zhiqian Li, and Jiancan Feng
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chlorophyll breakdown ,ethylene ,VvERF111 ,transcriptional regulation ,Plant Science ,Horticulture - Abstract
The plant growth regulator ethylene influences rachis browning in grape (Vitis vinifera L.). Although the ethylene signaling pathway is well defined, there is limited knowledge on its mode of action during rachis browning. Here, we show that an ethylene response factor (VvERF111) positively regulates chlorophyll degradation in rachis by binding to a DRE motif in the promoter of VvCLH1. The expression of VvERF111 and VvCLH1 in rachis was induced by ethylene and inhibited by 1-methylcyclopropene (1-MCP). VvERF111 belongs to the ERF IX subfamily of the APETALA2/ethylene responsive factor (AP2/ERF) superfamily, shows transcriptional activity in yeast, and is localized in the nucleus and membrane. The transient overexpression of VvERF111 or chlorophyllase (VvCLH1) in grape leaves accelerated chlorophyll degradation. In VvERF111-overexpressing leaves, transcript levels of VvCLH1 were also increased. Our findings offer a deeper understanding of the transcriptional regulation of chlorophyll degradation during the rachis browning of grape. more...
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- 2023
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23. Transcriptome Analysis Identifies Genes Associated with Chlorogenic Acid Biosynthesis during Apple Fruit Development
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Hao Wang, Xianbo Zheng, Yao Wu, Wenduo Zhan, Yanfei Guo, Ming Chen, Tuanhui Bai, Jian Jiao, Chunhui Song, Shangwei Song, and Miaomiao Wang
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chlorogenic acid ,apple fruit ,gene expression ,Plant Science ,Horticulture ,transcriptome - Abstract
As a predominant phenolic compound in apple fruits, chlorogenic acid (CGA) benefits human health due to its various antioxidant properties. However, little has been known regarding the molecular mechanism underlying the CGA accumulation in apple fruits. In this study, we measured the CGA content and relative enzymes’ activities during fruit development in two different flesh-colored cultivars ‘Huashuo’ and ‘Red Love’. The CGA content in both cultivars decreased sharply from 30 days after full bloom (DAFB) to 60 DAFB. Notably, the CGA content in fruit flesh was relatively higher than that in the peel. Further, the activities of C3H and HCT enzymes downstream of the CGA biosynthesis showed the similar changing trend as CGA content. Based on the transcriptome data of ‘Huashuo’ fruit at 30 DAFB and 60 DAFB, 23 differentially expressed CGA synthesis-related genes were screened. Gene expression analyses further showed that MdHCT1/2/4/5/6 and MdC3H1/2/3 were positively correlated to the variation of CGA content in two cultivars. These findings establish a theoretical foundation for further mechanism study on CGA biosynthesis and provide guidance for nutrient improvement in apple breeding programs. more...
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- 2023
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24. Exogenous Arginine Treatment Maintains the Appearance and Nutraceutical Properties of Hard- and Soft-Seed Pomegranates in Cold Storage
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Jiangli Shi, Huifang Gao, Sa Wang, Wenjiang Wu, Ruiran Tong, Sen Wang, Ming Li, Zaihai Jian, Ran Wan, Qingxia Hu, Xianbo Zheng, and Yanhui Chen
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Nutrition and Dietetics ,Endocrinology, Diabetes and Metabolism ,Food Science - Abstract
Arginine is a natural preservative; however, its effects on the storage of different cultivars of pomegranates have not been investigated extensively. Therefore, the fruit quality of soft-seed Tunisia and hard-seed Yudazi pomegranates was investigated after treatment with arginine at four concentrations during cold storage for 80 days. Pomegranates treated with 1.0 mM arginine exhibited a relatively lower loss of vitamin C, soluble solid, total phenol, and anthocyanin contents in arils, together with a better fruit appearance. Combined with principal component analysis (PCA), the storage life of fruits treated with 1.0 mM arginine showed a higher correlation with antioxidant enzyme activity (e.g., superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT)) during the first 40 days of cold storage, whereas after 40 days of cold storage, storage life was more dependent on the integrity of the cell membrane affected by malondialdehyde (MDA) content, electrolyte leakage (EL), and hydrogen peroxide (H2O2) accumulation. Arginine treatment contributed significantly to the appearance and inner quality of the hard-seed pomegranate cv. Yudazi fruit during cold storage compared to those of soft-seed Tunisia. Taken together, arginine application combined with cold storage enhanced the nutraceutical properties and marketability of pomegranate fruits. more...
- Published
- 2021
25. De novo chromosome-level genome of a semi-dwarf cultivar of Prunus persica identifies the aquaporin PpTIP2 as responsible for temperature-sensitive semi-dwarf trait and PpB3-1 for flower type and size
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Xiaodong, Lian, Haipeng, Zhang, Chao, Jiang, Fan, Gao, Liu, Yan, Xianbo, Zheng, Jun, Cheng, Wei, Wang, Xiaobei, Wang, Xia, Ye, Jidong, Li, Langlang, Zhang, Zhiqian, Li, Bin, Tan, and Jiancan, Feng
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Prunus persica ,Plant Breeding ,Fruit ,Temperature ,Flowers ,Aquaporins ,Chromosomes ,Genome-Wide Association Study - Abstract
Peach (Prunus persica) is one of the most important fruit crops globally, but its cultivation can be hindered by large tree size. 'Zhongyoutao 14' (CN14) is a temperature-sensitive semi-dwarf (TSSD) cultivar which might be useful as breeding stock. The genome of CN14 was sequenced and assembled de novo using single-molecule real-time sequencing and chromosome conformation capture assembly. A high-quality genome was assembled and annotated, with 228.82 Mb mapped to eight chromosomes. Eighty-six re-sequenced F more...
- Published
- 2021
26. Phenylalanine 4-Hydroxylase Contributes to Endophytic Bacterium Pseudomonas fluorescens’ Melatonin Biosynthesis
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Jian Jiao, Yan Xia, Yingli Zhang, Xueli Wu, Chonghuai Liu, Jiancan Feng, Xianbo Zheng, Shangwei Song, Tuanhui Bai, Chunhui Song, Miaomiao Wang, and Hongguang Pang
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endocrine system ,Antioxidant ,medicine.medical_treatment ,Pseudomonas fluorescens ,Phenylalanine ,melatonin ,QH426-470 ,Microbiology ,Melatonin ,chemistry.chemical_compound ,Biosynthesis ,medicine ,Genetics ,Genetics (clinical) ,aromatic amino acid hydroxylases ,biology ,Abiotic stress ,phenylalanine 4-hydroxylase ,biology.organism_classification ,endophytic bacteria ,chemistry ,5-hydroxytryptophan ,Molecular Medicine ,5-Hydroxytryptophan ,Bacteria ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug - Abstract
Melatonin acts both as an antioxidant and as a growth regulatory substance in plants. Pseudomonas fluorescens endophytic bacterium has been shown to produce melatonin and increase plant resistance to abiotic stressors through increasing endogenous melatonin. However, in bacteria, genes are still not known to be melatonin-related. Here, we reported that the bacterial phenylalanine 4-hydroxylase (PAH) may be involved in the 5-hydroxytryptophan (5-HTP) biosynthesis and further influenced the subsequent production of melatonin in P. fluorescens. The purified PAH protein of P. fluorescens not only hydroxylated phenylalanine but also exhibited l-tryptophan (l-Trp) hydroxylase activity by converting l-Trp to 5-HTP in vitro. However, bacterial PAH displayed lower activity and affinity for l-Trp than l-phenylalanine. Notably, the PAH deletion of P. fluorescens blocked melatonin production by causing a significant decline in 5-HTP levels and thus decreased the resistance to abiotic stress. Overall, this study revealed a possible role for bacterial PAH in controlling 5-HTP and melatonin biosynthesis in bacteria, and expanded the current knowledge of melatonin production in microorganisms. more...
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- 2021
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27. Overexpressing VvWRKY18 from grapevine reduces the drought tolerance in Arabidopsis by increasing leaf stomatal density
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Langlang Zhang, Rui Zhang, Xia Ye, Xianbo Zheng, Bin Tan, Wei Wang, Zhiqian Li, Jidong Li, Jun Cheng, and Jiancan Feng
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Plant Leaves ,Physiology ,Gene Expression Regulation, Plant ,Stress, Physiological ,Arabidopsis ,Plant Science ,Hydrogen Peroxide ,Plants, Genetically Modified ,Agronomy and Crop Science ,Abscisic Acid ,Droughts ,Plant Proteins - Abstract
The growth of grapevine [Vitis vinifera L.] is commonly limited by drought stress. The mechanisms by which grapevine copes with drought stress have not yet been extensively clarified. In this study, the drought and abscisic acid (ABA)-induced gene VvWRKY18 was demonstrated to decreased drought tolerance of Arabidopsis thaliana overexpression (VvWRKY18-OE) lines. Compared to wild-type plants, VvWRKY18-OE lines showed increased levels of malonaldehyde (MDA) and the reactive oxygen species (ROS) H more...
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- 2021
28. Phenylalanine 4-Hydroxylase Contributes to Endophytic Bacterium
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Jian, Jiao, Yan, Xia, Yingli, Zhang, Xueli, Wu, Chonghuai, Liu, Jiancan, Feng, Xianbo, Zheng, Shangwei, Song, Tuanhui, Bai, Chunhui, Song, Miaomiao, Wang, and Hongguang, Pang
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endocrine system ,endophytic bacteria ,5-hydroxytryptophan ,Genetics ,melatonin ,phenylalanine 4-hydroxylase ,hormones, hormone substitutes, and hormone antagonists ,Original Research ,aromatic amino acid hydroxylases - Abstract
Melatonin acts both as an antioxidant and as a growth regulatory substance in plants. Pseudomonas fluorescens endophytic bacterium has been shown to produce melatonin and increase plant resistance to abiotic stressors through increasing endogenous melatonin. However, in bacteria, genes are still not known to be melatonin-related. Here, we reported that the bacterial phenylalanine 4-hydroxylase (PAH) may be involved in the 5-hydroxytryptophan (5-HTP) biosynthesis and further influenced the subsequent production of melatonin in P. fluorescens. The purified PAH protein of P. fluorescens not only hydroxylated phenylalanine but also exhibited l-tryptophan (l-Trp) hydroxylase activity by converting l-Trp to 5-HTP in vitro. However, bacterial PAH displayed lower activity and affinity for l-Trp than l-phenylalanine. Notably, the PAH deletion of P. fluorescens blocked melatonin production by causing a significant decline in 5-HTP levels and thus decreased the resistance to abiotic stress. Overall, this study revealed a possible role for bacterial PAH in controlling 5-HTP and melatonin biosynthesis in bacteria, and expanded the current knowledge of melatonin production in microorganisms. more...
- Published
- 2021
29. Genome-wide identification and transcriptome profiling reveal that E3 ubiquitin ligase genes relevant to ethylene, auxin and abscisic acid are differentially expressed in the fruits of melting flesh and stony hard peach varieties
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Xia Ye, Xiaodong Lian, Jidong Li, Jiancan Feng, Bin Tan, Jun Cheng, Wenfang Zeng, Xianbo Zheng, Zhiqian Li, Langlang Zhang, and Wei Wang
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Subfamily ,lcsh:QH426-470 ,Ubiquitin-Protein Ligases ,lcsh:Biotechnology ,Flesh texture ,Genome ,Chromosomes, Plant ,Transcriptome ,The UPS pathway ,Peach (Prunus persica) ,Ubiquitin ,Auxin ,Gene Duplication ,lcsh:TP248.13-248.65 ,Genetics ,Gene ,Phylogeny ,Prunus persica ,chemistry.chemical_classification ,DNA ligase ,Indoleacetic Acids ,biology ,Gene Expression Profiling ,food and beverages ,Ethylenes ,Fruit ripening ,Ubiquitin protein ligase gene family ,Ubiquitin ligase ,lcsh:Genetics ,chemistry ,Fruit ,biology.protein ,Genome, Plant ,Abscisic Acid ,Research Article ,Biotechnology - Abstract
Background Ubiquitin ligases (E3) are the enzymes in the ubiquitin/26S proteasome pathway responsible for targeting proteins to the degradation pathway and play major roles in multiple biological activities. However, the E3 family and their functions are yet to be identified in the fruit of peach. Results In this study, genome-wide identification, classification and characterization of the E3 ligase genes within the genome of peach (Prunus persica) was carried out. In total, 765 E3 (PpE3) ligase genes were identified in the peach genome. The PpE3 ligase genes were divided into eight subfamilies according to the presence of known functional domains. The RBX subfamily was not detected in peach. The PpE3 ligase genes were not randomly distributed among the 8 chromosomes, with a greater concentration on the longer chromosomes. The primary mode of gene duplication of the PpE3 ligase genes was dispersed gene duplication (DSD). Four subgroups of the BTB subfamily never characterized before were newly identified in peach, namely BTBAND, BTBBL, BTBP and BTBAN. The expression patterns of the identified E3 ligase genes in two peach varieties that display different types of fruit softening (melting flesh, MF, and stony hard, SH) were analyzed at 4 different stages of ripening using Illumina technology. Among the 765 PpE3 ligase genes, 515 (67.3%) were expressed (FPKM > 1) in the fruit of either MF or SH during fruit ripening. In same-stage comparisons, 231 differentially expressed genes (DEGs) were identified between the two peach cultivars. The number of DEGs in each subfamily varied. Most DEGs were members of the BTB, F-box, U-box and RING subfamilies. PpE3 ligase genes predicted to be involved in ethylene, auxin, or ABA synthesis or signaling and DNA methylation were differentially regulated. Eight PpE3 ligase genes with possible roles in peach flesh texture and fruit ripening were discussed. Conclusions The results of this study provide useful information for further understanding the functional roles of the ubiquitin ligase genes in peach. The findings also provide the first clues that E3 ligase genes may function in the regulation of peach ripening. more...
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- 2019
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30. A single nucleotide mutation in<scp>GID</scp>1cdisrupts its interaction with<scp>DELLA</scp>1 and causes a GA‐insensitive dwarf phenotype in peach
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Bin Tan, Xia Ye, Yajun Jiang, Wei Wang, Jidong Li, Mengmeng Zhang, Tingting Xiong, Jiancan Feng, Xianbo Zheng, Zijing Guo, and Jun Cheng
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0106 biological sciences ,0301 basic medicine ,Nonsynonymous substitution ,dwarfism ,Mutant ,Dwarfism ,Plant Science ,Biology ,01 natural sciences ,peach ,Transcriptome ,03 medical and health sciences ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,medicine ,Point Mutation ,Gibberellic acid ,Research Articles ,Plant Proteins ,Prunus persica ,Genetics ,DELLA protein ,food and beverages ,medicine.disease ,Phenotype ,Gibberellins ,Dwarfing ,030104 developmental biology ,GID1c ,chemistry ,Elongation ,transcriptome ,Agronomy and Crop Science ,gibberellic acid ,Research Article ,010606 plant biology & botany ,Biotechnology - Abstract
Summary Plant stature is one important factor that affects the productivity of peach orchards. However, little is known about the molecular mechanism(s) underlying the dwarf phenotype of peach tree. Here, we report a dwarfing mechanism in the peach cv. FenHuaShouXingTao (FHSXT). The dwarf phenotype of ‘FHSXT’ was caused by shorter cell length compared to the standard cv. QiuMiHong (QMH). ‘FHSXT’ contained higher endogenous GA levels than did ‘QMH’ and did not response to exogenous GA treatment (internode elongation). These results indicated that ‘FHSXT’ is a GA‐insensitive dwarf mutant. A dwarf phenotype‐related single nucleotide mutation in the gibberellic acid receptor GID1 was identified in ‘FHSXT’ (GID1c S191F), which was also cosegregated with dwarf phenotype in 30 tested cultivars. GID1cS191F was unable to interact with the growth‐repressor DELLA1 even in the presence of GA. ‘FHSXT’ accumulated a higher level of DELLA1, the degradation of which is normally induced by its interaction with GID1. The DELLA1 protein level was almost undetectable in ‘QMH’, but not reduced in ‘FHSXT’ after GA 3 treatment. Our results suggested that a nonsynonymous single nucleotide mutation in GID1c disrupts its interaction with DELLA1 resulting in a GA‐insensitive dwarf phenotype in peach. more...
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- 2019
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31. Combination of iTRAQ proteomics and RNA-seq transcriptomics reveals jasmonate-related-metabolisms central regulation during the process of Jujube witches’ broom recovery by tetracycline treatment
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Jun Cheng, Jidong Li, Xianbo Zheng, Chen Peng, Wei Wang, Yajun Jiang, Xia Ye, Bin Tan, Huiyu Wang, and Jiancan Feng
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0106 biological sciences ,0301 basic medicine ,biology ,Tetracycline ,Jasmonic acid ,Broom ,RNA-Seq ,Horticulture ,biology.organism_classification ,01 natural sciences ,Microbiology ,Transcriptome ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Phytoplasma ,medicine ,Jasmonate ,Gene ,010606 plant biology & botany ,medicine.drug - Abstract
Witches’ broom disease, caused by phytoplasma, is currently the most destructive disease of jujube. Tetracycline derivatives have been used to treat witches’ broom, and can allow recovery from the phytoplasma infection. Genes related to jasmonic acid (JA) biosynthesis and JA-induced protein-like play roles in phytoplasma infection. Jujube shoots (Ziziphus jujuba Mill. ‘Huizao’) infected by phytoplasma were excised and grown in vitro before treatment with tetracycline. RNA-Seq and iTRAQ analyses of samples treated for different lengths of time were performed during recovery from jujube witches’ broom (JWB) symptoms and diminishing presence of phytoplasma. Phytoplasma was not detected by PCR in the shoots after 6 months of tetracycline treatment (MTT). RNA-Seq and iTRAQ analyses identified 26,790 genes and 6184 proteins from jujube shoot samples. There were 272 differentially expressed genes (DEGs) and 20 differentially expressed proteins (DEPs) between the 3 and 6 MTT samples, respectively. The largest number and the greatest changes of DEGs and DEPs were for those related to alpha-linolenic acid metabolism, jasmonate biosynthesis, and jasmonate induced protein-like (JIPs). JA content slightly decreased at the sixth month compared with the third month. The research avenues explored here showed that genes in the JA biosynthesis pathway, proteins that respond to JA (JIPs), and JA content itself were concurrently regulated during JWB recovery. Alpha-linolenic acid metabolism pathway had higher RichFactor in comparisons between the 3 and 6 MTT samples during JWB recovery, which suggesting JA played vital roles during JWB recovery. The results in this study will help us to understand the roles JA plays in host-phytoplasma interactions during recovery and infection of JWB. more...
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- 2019
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32. Contrasting Drought Tolerance in Two Apple Cultivars Associated with Difference in Leaf Morphology and Anatomy
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Tuanhui Bai, Xianbo Zheng, Jian Jiao, Zhanying Li, Yuchen Liu, Shangwei Song, Chunhui Song, and Zhidan Dong
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0106 biological sciences ,Stomatal conductance ,biology ,fungi ,Drought tolerance ,food and beverages ,Apple tree ,04 agricultural and veterinary sciences ,General Medicine ,biology.organism_classification ,01 natural sciences ,Photosynthetic capacity ,Honeycrisp ,Horticulture ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Cultivar ,Water-use efficiency ,010606 plant biology & botany ,Transpiration - Abstract
Apple is one of the most important fruit trees in temperate zones, and is cultivated widely throughout the world. Drought stress affects the normal growth of apple tree, and further affects fruit yield and quality. The present study examined the effects of drought on photosynthesis and water use efficiency (WUE) of two apple cultivars (Honeycrisp and Yanfu 3) that differ in drought tolerance. The results showed that the photosynthetic rate decreased in response to drought stress for both cultivars, with significant differences in intensity. Values for net photosynthetic rate (Pn) in stressed Yanfu 3 remained significantly lower than in the controls, while, for Honeycrisp, only a slight drop in photosynthesis. Similarly, stomatal conductance (Gs), intercellular CO2 concentration (Ci), transpiration rate (Tr) were markedly reduced in Yanfu 3 under drought stress. However, Honeycrisp showed only minor changes. Under drought stress, the contents of Chl a, Chl b and Chl t in Yanfu 3 were all decreased significantly compared with the control. However, little difference in Honeycrisp was noted between stressed plants and controls. Values for WUE in stressed Yanfu 3 remained higher than in the controls from day 3 until the end of the experiment, while no significant difference was observed in Honeycrisp. Furthermore, Honeycrisp also exhibited superior physiological traits, as indicated by its anatomical and morphological characteristics. Therefore, we conclude that the superior drought tolerance of Honeycrisp was due to its anatomical and morphological characteristics, which possibly contributed to the maintenance of higher photosynthetic capacity than Yanfu 3. more...
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- 2019
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33. VvERF95 regulates chlorophyll degradation by transcriptional activation of VvPAO1 causing grape rachis degreening after harvesting
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Zhiqian Li, Jingwen Li, Xia Ye, Xianbo Zheng, Bin Tan, Jidong Li, Jun Cheng, Wei Wang, Langlang Zhang, Xiaobei Wang, and Jiancan Feng
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Horticulture - Published
- 2022
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34. Overexpression of PgCBF3 and PgCBF7 Transcription Factors from Pomegranate Enhances Freezing Tolerance in Arabidopsis under the Promoter Activity Positively Regulated by PgICE1
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Lei Wang, Sa Wang, Ruiran Tong, Sen Wang, Jianan Yao, Jian Jiao, Ran Wan, Miaomiao Wang, Jiangli Shi, and Xianbo Zheng
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Inorganic Chemistry ,cold stress ,PgCBFs ,transcriptional regulation ,pomegranate ,Organic Chemistry ,General Medicine ,Physical and Theoretical Chemistry ,Molecular Biology ,Spectroscopy ,Catalysis ,Computer Science Applications - Abstract
Cold stress limits plant growth, development and yields, and the C-repeat binding factors (CBFs) function in the cold resistance in plants. However, how pomegranate CBF transcription factors respond to cold signal remains unclear. Considering the significantly up-regulated expression of PgCBF3 and PgCBF7 in cold-tolerant Punica granatum ‘Yudazi’ in comparison with cold-sensitive ‘Tunisia’ under 4 °C, the present study focused on the two CBF genes. PgCBF3 was localized in the nucleus, while PgCBF7 was localized in the cell membrane, cytoplasm, and nucleus, both owning transcriptional activation activity in yeast. Yeast one-hybrid and dual-luciferase reporter assay further confirmed that PgICE1 could specifically bind to and significantly enhance the activation activity of the promoters of PgCBF3 and PgCBF7. Compared with the wild-type plants, the PgCBF3 and PgCBF7 transgenic Arabidopsis thaliana lines had the higher survival rate after cold treatment; exhibited increased the contents of soluble sugar and proline, while lower electrolyte leakage, malondialdehyde content, and reactive oxygen species production, accompanying with elevated enzyme activity of catalase, peroxidase, and superoxide dismutase; and upregulated the expression of AtCOR15A, AtCOR47, AtRD29A, and AtKIN1. Collectively, PgCBFs were positively regulated by the upstream PgICE1 and mediated the downstream COR genes expression, thereby enhancing freezing tolerance. more...
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- 2022
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35. Ethylene and polyamines form a negative feedback loop to regulate peach fruit ripening via the transcription factor PpeERF113 by regulating the expression of PpePAO1
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Wei Wang, Shihao Liu, Xin Cheng, Zhenguo Cui, Yabo Jiang, Xianbo Zheng, Bin Tan, Jun Cheng, Xia Ye, Jidong Li, Zhiqian Li, Langlang Zhang, Xiaobei Wang, Haipeng Zhang, Xiaodong Lian, and Jiancan Feng
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Horticulture ,Agronomy and Crop Science ,Food Science - Published
- 2022
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36. Genome-wide identification of HSF family in peach and functional analysis of PpHSF5 involvement in root and aerial organ development
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Bin Tan, Jidong Li, Jun Cheng, Langlang Zhang, Xiaodong Lian, Huannan Li, Wang Xiaobei, Liu Yan, Xia Ye, Jiancan Feng, Wei Wang, Xianbo Zheng, and Zhiqian Li
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Genetics ,Oryza sativa ,biology ,PpHSF5 ,General Neuroscience ,Transgene ,lcsh:R ,Functional identification ,Nicotiana benthamiana ,food and beverages ,lcsh:Medicine ,General Medicine ,biology.organism_classification ,Heat shock factors family ,Aerial organ ,General Biochemistry, Genetics and Molecular Biology ,Root development ,Peach (Prunus persica) ,Arabidopsis ,Arabidopsis thaliana ,Gene family ,MYB ,General Agricultural and Biological Sciences ,Gene - Abstract
Background Heat shock factors (HSFs) play important roles during normal plant growth and development and when plants respond to diverse stressors. Although most studies have focused on the involvement of HSFs in the response to abiotic stresses, especially in model plants, there is little research on their participation in plant growth and development or on the HSF (PpHSF) gene family in peach (Prunus persica). Methods DBD (PF00447), the HSF characteristic domain, was used to search the peach genome and identify PpHSFs. Phylogenetic, multiple alignment and motif analyses were conducted using MEGA 6.0, ClustalW and MEME, respectively. The function of PpHSF5 was confirmed by overexpression of PpHSF5 into Arabidopsis. Results Eighteen PpHSF genes were identified within the peach genome. The PpHSF genes were nonuniformly distributed on the peach chromosomes. Seventeen of the PpHSFs (94.4%) contained one or two introns, except PpHSF18, which contained three introns. The in silico-translated PpHSFs were classified into three classes (PpHSFA, PpHSFB and PpHSFC) based on multiple alignment, motif analysis and phylogenetic comparison with HSFs from Arabidopsis thaliana and Oryza sativa. Dispersed gene duplication (DSD at 67%) mainly contributed to HSF gene family expansion in peach. Promoter analysis showed that the most common cis-elements were the MYB (abiotic stress response), ABRE (ABA-responsive) and MYC (dehydration-responsive) elements. Transcript profiling of 18 PpHSFs showed that the expression trend of PpHSF5 was consistent with shoot length changes in the cultivar ‘Zhongyoutao 14’. Further analysis of the PpHSF5 was conducted in 5-year-old peach trees, Nicotiana benthamiana and Arabidopsis thaliana, respectively. Tissue-specific expression analysis showed that PpHSF5 was expressed predominantly in young vegetative organs (leaf and apex). Subcellular localization revealed that PpHSF5 was located in the nucleus in N. benthamiana cells. Two transgenic Arabidopsis lines were obtained that overexpressed PpHSF5. The root length and the number of lateral roots in the transgenic seedlings were significantly less than in WT seedlings and after cultivation for three weeks. The transgenic rosettes were smaller than those of the WT at 2–3 weeks. The two transgenic lines exhibited a dwarf phenotype three weeks after transplanting, although there was no significant difference in the number of internodes. Moreover, the PpHSF5-OE lines exhibited enhanced thermotolerance. These results indicated that PpHSF5 might be act as a suppresser of growth and development of root and aerial organs. more...
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- 2021
37. Functional Analysis of the Gibberellin 2-oxidase Gene Family in Peach
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Jiancan Feng, Honglin Lv, Bin Tan, Jingjing Ma, Wei Wang, Mengmeng Zhang, Jidong Li, Xianbo Zheng, Langlang Zhang, Xia Ye, Jun Cheng, and Zhiqian Li
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Prunus persica ,Genetics ,Wild type ,food and beverages ,Plant Science ,Genetically modified crops ,lcsh:Plant culture ,Biology ,GA2ox ,peach ,Prunus ,functional divergence ,Shoot ,Gene family ,lcsh:SB1-1110 ,Gibberellin ,GA homeostasis ,Gene ,Functional divergence ,Original Research - Abstract
Peach (Prunus persica L. Batsch) trees grow vigorously and are subject to intense pruning during orchard cultivation. Reducing the levels of endogenous gibberellins (GAs) represents an effective method for controlling branch growth. Gibberellin 2-oxidases (GA2oxs) deactivate bioactive GAs, but little is known about the GA2ox gene family in peach. In this study, we identified seven PpGA2ox genes in the peach genome, which were clustered into three subgroups: C19-GA2ox-I, C19-GA2ox-II, and C20-GA2ox-I. Overexpressing representative genes from the three subgroups, PpGA2ox-1, PpGA2ox-5, and PpGA2ox-2, in tobacco resulted in dwarf plants with shorter stems and smaller leaves than the wild type. An analysis of the GA metabolic profiles of the transgenic plants showed that PpGA2ox-5 (a member of subgroup C19-GA2ox-II) is simultaneously active against both C19-GAs and C20-GAs,which implied that C19-GA2ox-II enzymes represent intermediates of C19-GA2oxs and C20-GA2oxs. Exogenous GA3 treatment of shoot tips activated the expression of all seven PpGA2ox genes, with different response times: the C19-GA2ox genes were transcriptionally activated more rapidly than the C20-GA2ox genes. GA metabolic profile analysis suggested that C20-GA2ox depletes GA levels more broadly than C19-GA2ox. These results suggest that the PpGA2ox gene family is responsible for fine-tuning endogenous GA levels in peach. Our findings provide a theoretical basis for appropriately controlling the vigorous growth of peach trees. more...
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- 2021
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38. Polyamine oxidase (PAO)–mediated polyamine catabolism plays potential roles in peach (Prunus persica L.) fruit development and ripening
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Jiancan Feng, Shihao Liu, Bin Tan, Jidong Li, Xianbo Zheng, Wei Wang, Jun Cheng, and Xia Ye
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0106 biological sciences ,0301 basic medicine ,Ethylene ,Catabolism ,food and beverages ,Forestry ,Ripening ,Horticulture ,Biology ,01 natural sciences ,03 medical and health sciences ,Prunus ,chemistry.chemical_compound ,Polyamine Catabolism ,030104 developmental biology ,chemistry ,Biochemistry ,Genetics ,Polyamine ,Molecular Biology ,Polyamine oxidase ,Gene ,010606 plant biology & botany - Abstract
Recently, there is an increased interest in the function of polyamine (PA) catabolism during fruit ripening, but little is known about its role during the ripening of peach (Prunus persica L.). In this study, the contents of both free and conjugated PAs markedly decreased as the peach fruit ripened. However, RNA-seq analysis showed that the transcript levels of PA synthesis–related genes increased, suggesting that the decreasing PA content during peach ripening was associated with PA catabolism. To better understanding the function of PA catabolism in peach ripening, genes encoding potential polyamine oxidases (PAOs), which are involved in PA catabolism, were identified across the peach genome. Four putative PAO genes (PpePAO1-PpePAO4) were identified in peach using the recently released genome database. The mRNA level of PpePAO1 was significantly increased during peach fruit ripening. Detection of free PAs in tobacco leaves transiently over-expressing PpePAO1 suggested that this gene is probably involved in terminal catabolism of PA. Treatment with the PAO inhibitor guazatine significantly reduced ethylene production and flesh softening of peach fruit, decreased the expression levels of fruit ripening-related genes, and significantly decreased the expression level of PpePAO1 and PAO activity, while PA contents were dramatically higher compared to control treatments. Our results suggests that PpePAO1-mediated PA catabolism is associated with decreased PA content during peach fruit ripening. These data provide valuable knowledge for better understanding the roles of PA catabolism in peach development and ripening. more...
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- 2021
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39. Genome-wide analysis of the GRAS transcription factor gene family in peach (Prunus persica) and ectopic expression of PpeDELLA1 and PpeDELLA2 in Arabidopsis result in dwarf phenotypes
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Chao Jiang, Fan Gao, Tianhao Li, Tanxing Chen, Xianbo Zheng, Xiaodong Lian, Xiaobei Wang, Haipeng Zhang, Jun Cheng, Wei Wang, Xia Ye, Jidong Li, Bin Tan, and Jiancan Feng
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Horticulture - Published
- 2022
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40. Widely targeted secondary metabolomics explored pomegranate aril browning during cold storage
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Jiangli Shi, Sen Wang, Ruiran Tong, Sa Wang, Yanhui Chen, Wenjiang Wu, Fengzhen He, Ran Wan, Zaihai Jian, Qingxia Hu, and Xianbo Zheng
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Horticulture ,Agronomy and Crop Science ,Food Science - Published
- 2022
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41. Genome-Wide Characterization of the HSP20 Gene Family Identifies Potential Members Involved in Temperature Stress Response in Apple
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Miaomiao Wang, Chunhui Song, Tuanhui Bai, Xianbo Zheng, Jian Jiao, Shangwei Song, Hongtao Wang, and Fuwen Yao
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0301 basic medicine ,Malus ,lcsh:QH426-470 ,Sequence analysis ,apple ,Biology ,Genome ,heat stress ,03 medical and health sciences ,0302 clinical medicine ,HSP20 family ,Heat shock protein ,Genetics ,Gene family ,Gene ,Genetics (clinical) ,Synteny ,fungi ,Intron ,biology.organism_classification ,equipment and supplies ,lcsh:Genetics ,030104 developmental biology ,030220 oncology & carcinogenesis ,gene expression ,Molecular Medicine ,bacteria ,genome-wide analysis - Abstract
Apple (Malus domestica Borkh.), an economically important tree fruit worldwide, frequently suffers from temperature stress during growth and development, which strongly affects the yield and quality. Heat shock protein 20 (HSP20) genes play crucial roles in protecting plants against abiotic stresses. However, they have not been systematically investigated in apple. In this study, we identified 41 HSP20 genes in the apple 'Golden Delicious' genome. These genes were unequally distributed on 15 different chromosomes and were classified into 10 subfamilies based on phylogenetic analysis and predicted subcellular localization. Chromosome mapping and synteny analysis indicated that three pairs of apple HSP20 genes were tandemly duplicated. Sequence analysis revealed that all apple HSP20 proteins reflected high structure conservation and most apple HSP20 genes (92.6%) possessed no introns, or only one intron. Numerous apple HSP20 gene promoter sequences contained stress and hormone response cis-elements. Transcriptome analysis revealed that 35 of 41 apple HSP20 genes were nearly unchanged or downregulated under normal temperature and cold stress, whereas these genes exhibited high-expression levels under heat stress. Subsequent qRT-PCR results showed that 12 of 29 selected apple HSP20 genes were extremely up-regulated (more than 1,000-fold) after 4 h of heat stress. However, the heat-upregulated genes were barely expressed or downregulated in response to cold stress, which indicated their potential function in mediating the response of apple to heat stress. Taken together, these findings lay the foundation to functionally characterize HSP20 genes to unravel their exact role in heat defense response in apple. more...
- Published
- 2020
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42. Auxin and Its Interaction With Ethylene Control Adventitious Root Formation and Development in Apple Rootstock
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Chunhui Song, Zhidan Dong, Miaomiao Wang, Shangwei Song, Jian Jiao, Xianbo Zheng, and Tuanhui Bai
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0106 biological sciences ,0301 basic medicine ,Ethylene ,indole-3-butyric acid ,Plant Science ,lcsh:Plant culture ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,apple rootstock 3/21 ,Auxin ,ethylene ,Primordium ,lcsh:SB1-1110 ,Original Research ,adventitious root ,chemistry.chemical_classification ,biology ,Chemistry ,fungi ,food and beverages ,Indole-3-butyric acid ,biology.organism_classification ,Cell biology ,030104 developmental biology ,Plant hormone ,Elongation ,Signal transduction ,Rootstock ,auxin ,010606 plant biology & botany - Abstract
Adventitious root (AR) formation is indispensable for vegetative asexual propagation. Indole-3-butyric acid (IBA) functioned indirectly as precursor of IAA in regulating AR formation. Ethylene affects auxin synthesis, transport, and/or signaling processes. However, the interactions between auxin and ethylene that control AR formation in apple have not been elucidated. In this study, we investigated the effects of IBA and its interaction with ethylene on AR development in apple. The results revealed that IBA stimulated the formation of root primordia, increased the number of ARs, and upregulated expression of genes (MdWOX11, MdLBD16, and MdLBD29) involved in AR formation. Comparison of different periods of IBA application indicated that IBA was necessary for root primordium formation, while long time IBA treatment obviously inhibited root elongation. RNA-seq analysis revealed that many plant hormone metabolism and signal transduction related genes were differentially expressed. IBA stimulated the production of ethylene during AR formation. Auxin inhibiting ARs elongation depended on ethylene. Together, our results suggest that the inhibitory role of auxin on AR elongation in apples is partially mediated by stimulated ethylene production. more...
- Published
- 2020
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43. Genome-Wide Characterization of the
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Fuwen, Yao, Chunhui, Song, Hongtao, Wang, Shangwei, Song, Jian, Jiao, Miaomiao, Wang, Xianbo, Zheng, and Tuanhui, Bai
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heat stress ,HSP20 family ,fungi ,Genetics ,gene expression ,bacteria ,apple ,equipment and supplies ,Original Research ,genome-wide analysis - Abstract
Apple (Malus domestica Borkh.), an economically important tree fruit worldwide, frequently suffers from temperature stress during growth and development, which strongly affects the yield and quality. Heat shock protein 20 (HSP20) genes play crucial roles in protecting plants against abiotic stresses. However, they have not been systematically investigated in apple. In this study, we identified 41 HSP20 genes in the apple ‘Golden Delicious’ genome. These genes were unequally distributed on 15 different chromosomes and were classified into 10 subfamilies based on phylogenetic analysis and predicted subcellular localization. Chromosome mapping and synteny analysis indicated that three pairs of apple HSP20 genes were tandemly duplicated. Sequence analysis revealed that all apple HSP20 proteins reflected high structure conservation and most apple HSP20 genes (92.6%) possessed no introns, or only one intron. Numerous apple HSP20 gene promoter sequences contained stress and hormone response cis-elements. Transcriptome analysis revealed that 35 of 41 apple HSP20 genes were nearly unchanged or downregulated under normal temperature and cold stress, whereas these genes exhibited high-expression levels under heat stress. Subsequent qRT-PCR results showed that 12 of 29 selected apple HSP20 genes were extremely up-regulated (more than 1,000-fold) after 4 h of heat stress. However, the heat-upregulated genes were barely expressed or downregulated in response to cold stress, which indicated their potential function in mediating the response of apple to heat stress. Taken together, these findings lay the foundation to functionally characterize HSP20 genes to unravel their exact role in heat defense response in apple. more...
- Published
- 2020
44. Transcript profiling provides insights into molecular processes during shoot elongation in temperature-sensitive peach (Prunus persica)
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Xia Ye, Xiaodong Lian, Jidong Li, Chao Jiang, Jiancan Feng, Tanxing Chen, Bin Tan, Liu Yan, Jun Cheng, Xianbo Zheng, and Wei Wang
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Agricultural genetics ,0106 biological sciences ,0301 basic medicine ,lcsh:Medicine ,Biology ,01 natural sciences ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,Auxin ,Brassinosteroid ,lcsh:Science ,Abscisic acid ,Prunus persica ,chemistry.chemical_classification ,Multidisciplinary ,Gene Expression Profiling ,Jasmonic acid ,lcsh:R ,Temperature ,Chromosome Mapping ,Computational Biology ,Molecular Sequence Annotation ,Cell biology ,Phenotype ,030104 developmental biology ,chemistry ,Shoot ,Cytokinin ,lcsh:Q ,Gene expression ,Elongation ,Transcriptome ,Plant Shoots ,Salicylic acid ,010606 plant biology & botany - Abstract
Plant growth caused by ambient temperature is thought to be regulated by a complex transcriptional network. A temperature-sensitive peach (Prunus persica) was used to explore the mechanisms behind shoot internode elongation at elevated temperatures. There was a significantly positive correlation between the length of the terminal internode (TIL) and the maximum temperature three days prior to the measuring day. Four critical growth stages (initial period and initial elongation period at lower temperature, rapid growth period and stable growth period at higher temperature) were selected for comparative RNA-seq analysis. About 6.64G clean bases were obtained for each library, and 88.27% of the data were mapped to the reference genome. Differentially expressed gene (DEG) analysis among the three pairwise comparisons resulted in the detection of several genes related to the shoot elongation in temperature-sensitive peach. HSFAs were up-regulated in response to the elevated temperature, while the up-regulated expression of HSPs might influence hormone signaling pathways. Most of DEGs involved in auxin, abscisic acid and jasmonic acid were up-regulated, while some involved in cytokinin and brassinosteroid were down-regulated. Genes related to ethylene, salicylic acid and circadian rhythm were also differentially expressed. Genes related to aquaporins, expansins, pectinesterases and endoglucanase were up-regulated, which would promote cell elongation. These results lay a foundation for further dissection of the regulatory mechanisms underlying shoot elongation at elevated temperatures. more...
- Published
- 2020
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45. Genome-wide identification of HSF family in peach and functional analysis of
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Bin, Tan, Liu, Yan, Huannan, Li, Xiaodong, Lian, Jun, Cheng, Wei, Wang, Xianbo, Zheng, Xiaobei, Wang, Jidong, Li, Xia, Ye, Langlang, Zhang, Zhiqian, Li, and Jiancan, Feng
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Peach (Prunus persica) ,PpHSF5 ,Functional identification ,food and beverages ,Genomics ,Plant Science ,Agricultural Science ,Heat shock factors family ,Molecular Biology ,Aerial organ ,Biotechnology ,Root development - Abstract
Background Heat shock factors (HSFs) play important roles during normal plant growth and development and when plants respond to diverse stressors. Although most studies have focused on the involvement of HSFs in the response to abiotic stresses, especially in model plants, there is little research on their participation in plant growth and development or on the HSF (PpHSF) gene family in peach (Prunus persica). Methods DBD (PF00447), the HSF characteristic domain, was used to search the peach genome and identify PpHSFs. Phylogenetic, multiple alignment and motif analyses were conducted using MEGA 6.0, ClustalW and MEME, respectively. The function of PpHSF5 was confirmed by overexpression of PpHSF5 into Arabidopsis. Results Eighteen PpHSF genes were identified within the peach genome. The PpHSF genes were nonuniformly distributed on the peach chromosomes. Seventeen of the PpHSFs (94.4%) contained one or two introns, except PpHSF18, which contained three introns. The in silico-translated PpHSFs were classified into three classes (PpHSFA, PpHSFB and PpHSFC) based on multiple alignment, motif analysis and phylogenetic comparison with HSFs from Arabidopsis thaliana and Oryza sativa. Dispersed gene duplication (DSD at 67%) mainly contributed to HSF gene family expansion in peach. Promoter analysis showed that the most common cis-elements were the MYB (abiotic stress response), ABRE (ABA-responsive) and MYC (dehydration-responsive) elements. Transcript profiling of 18 PpHSFs showed that the expression trend of PpHSF5 was consistent with shoot length changes in the cultivar ‘Zhongyoutao 14’. Further analysis of the PpHSF5 was conducted in 5-year-old peach trees, Nicotiana benthamiana and Arabidopsis thaliana, respectively. Tissue-specific expression analysis showed that PpHSF5 was expressed predominantly in young vegetative organs (leaf and apex). Subcellular localization revealed that PpHSF5 was located in the nucleus in N. benthamiana cells. Two transgenic Arabidopsis lines were obtained that overexpressed PpHSF5. The root length and the number of lateral roots in the transgenic seedlings were significantly less than in WT seedlings and after cultivation for three weeks. The transgenic rosettes were smaller than those of the WT at 2–3 weeks. The two transgenic lines exhibited a dwarf phenotype three weeks after transplanting, although there was no significant difference in the number of internodes. Moreover, the PpHSF5-OE lines exhibited enhanced thermotolerance. These results indicated that PpHSF5 might be act as a suppresser of growth and development of root and aerial organs. more...
- Published
- 2020
46. A sensitive visual method for onsite detection of quarantine pathogenic bacteria from horticultural crops using an LbCas12a variant system
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Jian Jiao, Mengjie Yang, Tengfei Zhang, Yingli Zhang, Mengli Yang, Ming Li, Chonghuai Liu, Shangwei Song, Tuanhui Bai, Chunhui Song, Miaomiao Wang, Hongguang Pang, Jiancan Feng, and Xianbo Zheng
- Subjects
Citrullus ,Crops, Agricultural ,DNA, Bacterial ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,Quarantine ,Environmental Chemistry ,Real-Time Polymerase Chain Reaction ,Pollution ,Waste Management and Disposal - Abstract
Pre-planting testing of seeds and plantlets for the existence of quarantine pathogens is an important phytosanitary measure. The CRISPR-mediated molecular diagnostic methodologies are being developed for pathogens detection, but many challenges remain. Here, we profiled an engineered Crispr/LbCas12a variant (LbCas12a-5M) that has more robust trans-cleavage activity and a wider PAM sequences (TNTN) preference than wild type. We developed a procedure for screening specific sequences of bacterial plant pathogens, and the designed species-specific crRNA displayed no cross-reactions with other bacterial species. Combined with a simple extraction of bacterial DNA, an LbCas12a-5M-based visual detection technique was established and optimized for detecting quarantine pathogens Erwinia amylovora and Acidovorax citrulli with detection limits up to 40 CFU/reaction and a sensitivity consistent with qPCR assay. This protocol was faster and simpler than qPCR, requiring 40 min or less from sample preparation. We further validated the potential application of the method by showing that it can be used for rapid and accurate diagnosis of A. citrulli on seeds of watermelon, with 100% agreement with the results of qPCR assay. The developed method simplifies the detection of pathogens and provides cost-effective countermeasures to quarantine interventions. more...
- Published
- 2022
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47. Genome‑wide identification and expression analysis of the ASMT gene family reveals their role in abiotic stress tolerance in apple
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Shangwei Song, Sen Fang, Jian Jiao, Zhengyang Wang, Chunhui Song, Hongtao Wang, Miaomiao Wang, Xianbo Zheng, and Tuanhui Bai
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Genetics ,Malus ,Abiotic stress ,Sequence analysis ,fungi ,Horticulture ,Biology ,biology.organism_classification ,Genome ,Transcriptome ,Acetylserotonin O-methyltransferase ,bacteria ,Gene family ,Gene - Abstract
Apple (Malus domestica Borkh.), an economically important fruit crop, is widely cultivated worldwide. However, apple trees often suffer from environmental stressors which affect the quality and yield of apple fruit. Acetylserotonin methyltransferase (ASMT) is a key enzyme in melatonin biosynthesis and is known to play critical roles in melatonin production and stress responses of plants. In this study, a total of 37 ASMT genes in apple were identified and unequally distributed on 10 apple chromosomes, with fragment duplication detected in four pairs of genes. The ASMT genes were classified into three groups based on their sequence compositions and phylogenetic relationship. Sequence analysis showed that apple ASMT genes were generally conserved in each group, with minor variations in gene structure and motif distribution. Transcriptome analysis revealed that most apple ASMT genes were nearly unchanged or downregulated under heat, cold, drought, and NaCl stress, whereas MdASMT6, MdASMT11, MdASMT14 and MdASMT19 were highly induced by these stressors. Subsequent qRT-PCR of four apple ASMT genes showed that MdASMT11 and MdASMT14 were extremely up-regulated under heat, cold, drought, and NaCl stress. They were expressed in all tissues, especially in the roots. Subcellular localization showed that MdASMT11 mainly distributed on cell membranes and MdASMT14 mainly anchored to the nucleus and cell membrane, indicating that MdASMT11 and MdASMT14 are potential genes regulating abiotic stress resistance in apple. These results facilitate further investigation to the functional characterization of MdASMT genes in the synthesis of melatonin and the response to abiotic stress and in apple. more...
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- 2022
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48. Expression of grape ACS1 in tomato decreases ethylene and alters the balance between auxin and ethylene during shoot and root formation
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Jiancan Feng, Yu Liu, Xianbo Zheng, Jidong Li, Wei Wang, Dongliang An, Bin Tan, Mengmeng Fu, Xia Ye, and Jun Cheng
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0106 biological sciences ,0301 basic medicine ,Ethylene ,Physiology ,Lyases ,Plant Science ,Berry ,Biology ,Plant Roots ,01 natural sciences ,Ectopic Gene Expression ,03 medical and health sciences ,chemistry.chemical_compound ,Solanum lycopersicum ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Auxin ,Vitis ,Genetically modified tomato ,Plant Proteins ,chemistry.chemical_classification ,Indoleacetic Acids ,fungi ,food and beverages ,Ethylenes ,Plants, Genetically Modified ,biology.organism_classification ,Horticulture ,030104 developmental biology ,chemistry ,Shoot ,Ectopic expression ,Zeatin ,Solanum ,Agronomy and Crop Science ,Plant Shoots ,010606 plant biology & botany - Abstract
Ethylene plays an important role in the grape rachis, where its production can be 10 times higher than in the berry. VvACS1 is the only rachis-specific ACC synthase (ACS) gene, and its expression is coincident with ethylene production in the rachis of Vitis vinifera 'Thompson seedless'. VvACS1 was cloned and ectopically expressed in tomato (Solanum lycopersicum 'Moneymaker'). Lateral buds were increased in two- or four-week-old 35s∷VvACS1 transgenic tomato plants after transplanting. Compared with wild-type (WT) plants, the transgenic tomato plants showed higher expression of the VvACS1 gene in the flowers, leaves, rachis, and fruits. There was no obvious difference of ACS activity in the fruit of tomato, and only increased ACS activity in the rachis of tomato. Ethylene production was decreased in flowers, leaves, and fruits (seven weeks after full bloom), while the relative expression of endogenous tomato ACS1 and ACS6 genes was not down-regulated by the ectopic expression of VvACS1. These results imply that post-transcriptional or post-translational regulation of ACS may occur, resulting in lower ethylene production in the transgenic tomato plants. Moreover, expression of VvACS1 in tomato resulted in decreased auxin and increased zeatin contents in the lateral buds, as well as reduced or delayed formation of adventitious roots in lateral bud cuttings. RNA-Seq and qRT-PCR analyses of rooted lateral bud cuttings indicated that the relative expression levels of the genes for zeatin O-glucosyltransferase-like, auxin repressed/dormancy-associated protein, and ERF transcription factors were higher in transgenic tomatoes than in WT, suggesting that ethylene may regulate auxin transport and distribution in shoots and that adventitious root formation employs coordination between auxin and ethylene. more...
- Published
- 2018
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49. Transcriptome profiling analysis revealed co-regulation of multiple pathways in jujube during infection by ‘ Candidatus Phytoplasma ziziphi ’
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Wei Wang, Chen Peng, Huiyu Wang, Bin Tan, Jidong Li, Xia Ye, Jiancan Feng, Xianbo Zheng, and Jun Cheng
- Subjects
0301 basic medicine ,Phytoplasma ,Germination ,03 medical and health sciences ,chemistry.chemical_compound ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Auxin ,Genetics ,Brassinosteroid ,Secondary metabolism ,Abscisic acid ,Plant Diseases ,Plant Proteins ,chemistry.chemical_classification ,Phenylpropanoid ,biology ,Gene Expression Profiling ,Jasmonic acid ,food and beverages ,Ziziphus ,General Medicine ,biology.organism_classification ,030104 developmental biology ,Flavonoid biosynthesis ,chemistry ,Signal Transduction - Abstract
Background Jujube witches' broom (JWB), caused by a phytoplasma, devastates jujube tree (Ziziphus jujuba) growth and production in Asia. Although host responses to phytoplasmas are studied at the phenotypic, physiological, biochemical and molecular levels, it remains unclear how a host plant responds at the molecular level during the primary stage of infection. Methods To understand the response of the jujube tree to JWB infection, leaves were sampled at different times during the phytoplasma infection. Transcriptomic analyses at six stages were performed to reveal how phytoplasma infection affects Chinese jujube gene expression through the determination of the key differentially expressed genes (DEGs), and their related pathways. Quantitative real-time PCR was applied to validate 10 differentially expressed genes at different JWB phytoplasma infection stages. Results A total of 25,067 unigenes were mapped to jujube genome reference sequences. In the first infection stage (0–2 weeks after grafting (WAG), a total of 582 jujube genes were differentially regulated but no visible symptoms appeared. Quite a few DEGs related to abscisic acid (ABA) and cytokinin (CTK) were down-regulated, while some related to jasmonic acid (JA) and salicylic acid (SA) were up-regulated, Genes related to plant-pathogen interaction were also differentially expressed. In the second infection stage (37–39WAG), witches' broom symptoms were visible, and a total of 4373 DEGs were identified. Genes involved in biosynthesis and signal transduction of ABA, brassinosteroid (BR), CTK, ethylene (ET), and auxin (IAA), GA, JA and SA, plant-pathogen interaction, flavonoid biosynthesis genes were significantly regulated, suggesting that jujube trees activated defense factors related to SA, JA, ABA and secondary metabolites to defend against phytoplasma infection. By the third infection stage (48–52WAG), serious symptoms occurred and 3386 DEGs were identified. Most DEGs involved in biosynthesis and signal transduction of JA, SA and GA were up-regulated, while those relating to ABA were down-regulated. Genes involved in plant-pathogen interaction were up- or down-regulated, while phenylpropanoid and flavonoid biosynthesis genes were significantly up-regulated. Meanwhile, DEGs involved in photosynthesis, chlorophyll and peroxisome biosynthesis, and carbohydrate metabolism were down-regulated. These results suggested that phytoplasma infection had completely destroyed jujube trees' defense system and had disturbed chlorophyll synthesis and photosynthetic activity in the infected leaves at the late stage, resulting in yellow leaves and other JWB symptoms. Discussion The results in this report suggested that phytohormone biosynthesis and signal transduction, photosynthesis, and secondary metabolism all played important roles in the battle between colonization and defense in the interaction between Ca. Phytoplasma ziziphi and jujube. more...
- Published
- 2018
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50. Genome-wide identification of microRNAs involved in the regulation of fruit ripening in apple (Malus domestica)
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Shangwei Song, Jian Jiao, Xianbo Zheng, Tuanhui Bai, Tong-Xin Li, Chunhui Song, Miaomiao Wang, and Yao Wu
- Subjects
Genetics ,Malus ,microRNA ,Postharvest ,food and beverages ,Identification (biology) ,Ripening ,Horticulture ,Biology ,biology.organism_classification ,Genome ,Transcription factor ,WRKY protein domain - Abstract
Fruit ripening is a complicated process that is influenced by diverse factors at different regulation levels. The microRNAs (miRNAs), a class of endogenous small noncoding RNAs, are recently demonstrated to regulate several critical biological processes in various aspects of the fruit's life cycle. In this paper, to gain more insights into the regulatory mechanism of miRNAs in apple fruit ripening, a genome-wide identification of the sRNAome was implemented in the postharvest ‘Qinguan’ apple fruit. The ripening process of the postharvest apple fruit was significantly inhibited via the application of 1-MCP (1 µL/L). Totally, 569 miRNAs were successfully identified, including 132 known and 437 novel miRNAs, and 29 of them were significantly differentially expressed. The mdm-miR398a, mdm-miR395i-3p, mdm-miR395b, mdm-miR395d-3p, mdm-miR395h, mdm-miR395g-3p, and the novel-miR156 were identified to be the differentially expressed miRNAs. Moreover, after parsing the targets of these miRNAs, several transcription factors, such as ERF and WRKY, were found to be involved in the apple fruit ripening. The above results provide new information for understanding the sophisticated coordinated regulatory network of apple fruit ripening. more...
- Published
- 2021
- Full Text
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