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The crisis of arsenic (As) accumulation in rhizomes threatens the quality and safety of Panax notoginseng(Burk.) F.H. Chen, which is a well-known traditional Chinese herb with a long clinical history. The uptake of arsenate (AsV) could be suppressed by supplying phosphate (Pi), in which Pi transporters play important roles in the uptake of Pi and AsV. Herein, the P. notoginseng Pi transporter-encoding gene PnPht1;3 was identified and characterised under Pi deficiency and AsV exposure. In this study, the open reading frame (ORF) of PnPht1;3 was cloned according to RNA-seq and encoded 545 amino acids. The relative expression levels revealed that PnPht1;3 was significantly upregulated under phosphate deficiency and AsV exposure. Heterologous expression in Saccharomyces cerevisiae MB192 demonstrated that PnPht1;3 performed optimally in complementing the yeast Pi-transport defect and accumulated more As in the cells. Combined with the subcellular localisation prediction, it was concluded that PnPht1;3 encodes a functional plasma membrane-localised transporter protein that mediates putative high-affinity Pi/H+ symport activity and enhances the uptake of Pi and AsV. Therefore, a better understanding of the roles of the P. notoginseng Pi transporter could provide new insight for solving As accumulation in medicinal plants.

Background Panax notoginseng is a medicinally important Chinese herb with a long history of cultivation and clinical application. The planting area is mainly distributed in Wenshan Prefecture, where the quality and safety of P. notoginseng have been threatened by high concentration of arsenic (As) from the soil. The roles of phosphate (Pi) transporters involved in Pi acquisition and arsenate (AsV) tolerance were still unclear in this species. Results In this study, two open reading frames (ORFs) of PnPht1;1 and PnPht1;2 separated from P. notoginseng were cloned based on RNA-seq, which encoded 527 and 541 amino acids, respectively. The results of relative expression levels showed that both genes responded to the Pi deficiency or As exposure, and were highly upregulated. Heterologous expression in Saccharomyces cerevisiae MB192 revealed that PnPht1;1 and PnPht1;2 performed optimally in complementing the yeast Pi-transport defect, particularly in PnPht1;2. Cells expressing PnPht1;2 had a stronger AsV tolerance than PnPht1;1-expressing cells, and accumulated less As in cells under a high-Pi concentration. Combining with the result of plasma membrane localization, these data confirmed that transporters PnPht1;1 and PnPht1;2 were putative high-affinity H+/H2PO4− symporters, mediating the uptake of Pi and AsV. Conclusion PnPht1;1 and PnPht1;2 encoded functional plasma membrane-localized transporter proteins that mediated a putative high-affinity Pi/H+ symport activity. Expression of PnPht1;1 or PnPht1;2 in mutant strains could enhance the uptake of Pi and AsV, that is probably responsible for the As accumulation in the roots of P. notoginseng.

The robustness of Freight Comprehensive Transportation Networks (FCTNs) refers to the ability that FCTNs can maintain their function or performance tolerating perturbations from uncertainties. To evaluate the robustness of FCTNs, this paper proposes several quantitative indicators including accessibility, delivery, carrying capacity and transferring capacity. Numerical results based on the case of coal comprehensive transportation networks in Shanxi, China show the effectiveness of the proposed method.

Recent studies have reported that miRNAs might play critical roles in acute myocardial infarction (AMI). The objective of this study is to investigate the role of miR-499-5p in AMI and its potential molecular mechanisms. The expression level of MiR-499-5p was remarkably decreased in the infarcted myocardial tissues and in the cultured neonatal rat cardiomyocytes induced by hypoxia. Overexpression or knockdown of miR-499-5p decreased or increased the apoptotic rates of cultured cardiomyocytes in vitro. In addition, ectopic overexpression of miR-499-5p in the rat AMI models with agomir reduced the myocardial infarct size through decreasing the cardiomyocytes apoptosis in the infarcted area of the rat hearts. PDCD4 (programmed cell death 4) was verified as a direct target of miR-499-5p by luciferase report assay, and ectopic overexpression or inhibition of miR-499-5p could inhibit or increase the PDCD4 expression at both the mRNA and protein levels. Furthermore, we found that ectopic overexpression of PDCD4 without miR-499-5p binding sites reversed miR-499-5p-mediated cardiomyocytes apoptosis. Together, these findings revealed the role of miR-499-5p in protecting the cardiomyocytes against apoptosis induced by AMI via its direct target PDCD4, which providing evidence for the miR-499-5p/PDCD4 pathway as a potential therapeutic target for patients with AMI.

Bicycle movements are always associated with lateral drifts. However, the impacts of lateral drift behavior, as well as variable lateral clearance maintaining behavior due to the variation of drift intensity, on mixed bicycle flow are not clear. This paper establishes a new cellular automata model to study typical characteristics of mixed bicycle traffic induced by lateral drift and its accompanying behavior. Based on derived positive correlation between passing speed and drift speed through survey, the occurrence probability of lateral drift and the degree of maintained lateral clearance are both introduced in accordance with the variance of passing speed. Then, in whole density region, firm conformity between simulation results and actual survey data is reached, which has seldom been achieved in previous studies. It is further verified that speed distortions in intermediate and high density region induced by assumption of constant lateral clearance requirements can be revised by introducing its variability characteristics. In addition, two contrastive impacts of lateral drift behavior are observed. That is, it causes speed fluctuation in low density while alleviating the speed fluctuation in relatively high density. These results are expected to be helpful to improve the simulation performance of mixed bicycle flow as well as depict more realistic vehicle-bicycle conflicts and so on.

To investigate the effects of cellular membrane connexin 43 (Cx43) and the potential details in ischemic postconditioning (IPOC)-induced cardioprotection, ischemia/reperfusion (IR) models were generated in 8-week-old male Sprague-Dawley rats by ligating the left coronary artery anterior descending branch. The serum levels of myocardial creatases, nitric oxide (NO) and malondialdehyde (MDA) levels, infarct size, arrhythmia events, expression and distribution of Cx43, ultrastructure and apoptosis in the myocardium in different treatments with IR, IR + IPOC, IR + diazoxide or IR + IPOC + 5-hydroxydecanoate acid (5-HD) were investigated. Consequently, IPOC decreased infarct size (10.9 vs. 43.3%, P0.05); however, it was reversed after 3-h reperfusion and the p-Cx43 level in the IR + IPOC group was significantly higher than that in the IR + diazoxide group (32.8 vs. 18.7%, P

According to the selection and evaluation of railway freight transport of the third party logistics, establish a complete set of fuzzy hierarchy evaluation (F-AHP) model of railway freight third party logistics company. Taking Nanchang Train Depot as an example of empirical analysis, Calculate and screen the most appropriate third party logistics company. At the same time, we confirm the reliability and authenticity of F-AHP method that applies to the third party logistics company, which provides reference experience for the railway freight department on the selection of third party logistics companies.

Background Hypoxemia sometimes occurs in the emergency room in the patients with acute myocardial infarction (AMI) after percutaneous coronary intervention (PCI), even in those with administration of conventional high-flow oxygen inhalation. The objective of the present study was to evaluate the effectiveness of non-invasive ventilation (NIV) in improving blood oxygen content and hemorheology in patients with AMI and hypoxemia. Methods This prospective study enrolled 50 consecutive eligible patients with AMI (aged 72.3 ± 9.5 years), who had undergone PCI and been administered high-flow oxygen but still had hypoxemia. Blood was taken before NIV and at 0.5, 1, and 2 h after NIV. Blood gases, hemorheological variables including erythrocyte deformability, erythrocyte aggregation, erythrocyte osmotic fragility, membrane fluidity, and oxidative stress level were measured. Results Blood PaO2 increased to normal by 1 h after NIV. Assessed hemorheological variables had all improved and plasma malondialdehyde concentration decreased significantly after 2 h of NIV. Conclusions Our data suggest that NIV can help to improve blood oxygen content, hemorheological status, and minimize plasma lipid peroxidation injury in hypoxemic patients with AMI who have undergone PCI.

Under the background of economic globalization and regional economic integration, B2C enterprises begin to focus on how to handle the relationship between logistics services and E-commerce services in the face of intense competition from other companies. This paper proposes a fuzzy analytical hierarchy process (FAHP) approach to select the logistics mode of B2C electronic commerce companies. In addition, a case study is presented to demonstrate how the approach can help in solving such problems in practice.

China has a "North coal to south, West coal to east" overall pattern of coal transportation. This paper focuses on how to optimize the distribution of supply and demand, which can effectively reduce the total transportation costs. In order to study the optimization problem, we build a linear programming model, and solve it through commonly used mathematical programming software CPLEX. Then, taking Shanxi as an example, the proposed model was verified. By introducing supply point capacity limits, make our model more adaptable to the actual situation.

In view of the new requirement of railway information service, such as information sharing, collaboration, etc. which occurred during the development of railway information construction, Grid computing was introduced into the field of railway information service, which analyzed the development trend of railway information service based on grid, made a research on the application of the grid computing in the field of railway, constructed railway application framework based on grid, proposed grid-based railway IT system and provided a theoretical basis and reference for the grid application in railway information service.

Local railway is mainly constructed by local investment or joint investment with other railways. It shoulders the transportation of local public passengers and short distance of railway goods, and bears the task of boosting local economy and domestic demands [1]. Choosing suitable local railway operation management mode could help to realize maximal economic benefits with least amount of investment, and best management. Through the analysis of China's local railway company operation and management model, this paper proposes five methods to guide how to choose local railway operation and management mode.

The paper builds the model of direct reuse reverse logistics center location followed by the uncertainty random variables of the demand of retailers and the recovery of collection points. This model assumes that the enterprises are expanding on the traditional network. For the random variables, they will be solved by using stochastic simulation, genetic algorithm and linear programming, and numericai exampie is presented.

Based on the essential characteristics of coal transportation network, the paper analyzed uncertainties of coal transportation network in 3 aspects ,structural uncertainty, time uncertainty and transport capacity uncertainty. It built a state equation of coal transportation network with the above three kinds of uncertainty, and established a robust control model of coal transportation network, to realize equilibrium and stability of coal flow in the coal transportation network. Finally, take Shanxi coal transportation network for example, to verify the accuracy and effectiveness of the coal transportation network robust control methods in the paper.

Hub location has been a fertile area for interdisciplinary researchers. There has been used to plan the number of hubs, position, grades, scale and so on. Many scholars have studied hub location problem for more than 30-year history which has indepth researched on the theoretical concepts, mathematical models and applications in hub location. For the development of hub location, a review of classification of HLPs is provided, and then mathematical models, resolution algorithm, the advantages and disadvantages of each classification could be described in detail. At the end, the future research trends will be presented.

The dissertation discusses the significance of this thesis of current Intelligent Transportation System forward from the present research as a starting point; secondly, by analyzing the existing problem, we describe the necessity of leading the IOT technology into ITS; finally, we can prove that the new architecture of ITS in IOT can surpass the existing Intelligent Transportation System.

Industry security system is a complex giant system, madding up of multiple subsystems, and presenting features of hierarchy and complexity. Based on the industrial security characteristics and requirements, using the theory and method of system science, this paper analyzes the related issues of industry security. And the research mainly regards to industry security system components and the correlation between each element. And the model of correlation for industry security system elements is put forward innovatively, which has certain practical significance.

Public bicycle rental system plays an important role in the construction of low-carbon city and the development of green traffic system. The reasonable layout of public bicycle station can ease the traffic jam effectively and improve the level of public bicycle service. This paper expounds the principles of bicycle station layout. With an analysis of the public bicycle traffic chain, classify the bicycle stations into 5 types and determine the relationship of each point. Finally, the paper built a bi-level model combined of feedback model spilt and traffic assignment model. The optimization model can make the travel and construction costs of the whole system get to the lowest, and it effectively solve the layout problem of bicycle rental stations.

This paper studies the problem of inventory and distribution joint optimization for two level logistics system of a supplier and multiple clients by building the model of inventory and distribution joint optimization based on VMI to reach the goal of minimizing the total system cost. Moreover, this article not only designs the iterative algorithm to solve the model, and determine the optimal inventory and distribution program from the perspective of system optimization, but also verifies the validity of the model and algorithm through using MATLAB software.

Based on the basic theory of complex networks, this paper introduces the basic concept and constitution of coal transport network, summarizs the characteristics of the coal transportation network.Then through principles of coal transportation network topology abstraction,we establishe the model of coal network topology, and analyses the internal and external complexity factors on coal transportation network.

In order to meet the demand for railway information service, Discussed the grid connotation and the theory of grid architecture, used Service-Oriented idea, the architecture of railway information service was proposed in the grid environment, the level of architecture was determined, Meanwhile composition, functions and the hierarchical relationship of the architecture were discussed in detail, the research focus on providing the top guidance for the construction of the railway information grid system.

The domain of railway is the most promising field when IOT applies in practice. By means of expounding on the connotation of railway information technology, carrying out research on the components of railway information technology and classifying it into categories, this paper constructs railway information technology system based on Internet of Things (IOT). It buttresses further application of the IOT in railway field.

Internet of Things (IOT) has the advantages of thorough perception, comprehensive connection and deep intelligent application etc., which provide support for the informational and intelligent development in railway. Oriented to the demand of IOT in railway, this paper focuses on the combination of railway and IOT technology. Based on the analysis of IOT technology and railway business demands, this paper establishes a framework of its technical applications in railway, and comes up the application frameworks in railway transport and dispatching, management of capacity resource, passenger and freight, safety, and information control.

SLP method is one kind of rational layout methods with strongly logicality and it stresses that logistics analysis is closely combined with the relationship of functional zones. Through repeated modifications and adjustments in the process of applying the method, the result has a strong practicality.Combining with the particularity of fresh products logistics, this paper improved the traditional SLP method including basic logistics elements analysis and calculating the area,and proved the new method through a case.

Following the rapid development of information technology in the field of railway transportation, the problems of establishing a digital, integrated and intelligent special railway system need to be solved immediately. This paper designs and implements the intelligent transportation information system based on the unique pattern of transportation organization, the characteristics of transportation operations and the workflow of special railway. Through the detailed analysis of system architecture and framework design, the main subsystems and the internal comprehensive integrated principle, business system from a system integration perspective of the special railway is optimized, which can be able to realize the integration of all kinds of information resources. The implementation of integration and the special railway intelligent system is a great change in terms of maximizing transportation capacity, improving efficiency and guaranteeing the safety of special railway transportation.

Internet of Things (IOT) has attracted the attention of various industries with its widely used, such as railway field in China. This paper analyses IOT concept and key technology, according to the rail cars daily management situation, proposes a rail cars daily management design by using IOT. It includes statistics for rail cars running distance, statistics for rail cars fuel consumption and control rail cars using data. It aims to promote management efficiency and improve the service quality.

When IOT applies in practice, the domain of railway is the most promising field. Through elaborating the essence of railway information sharing, combining Internet of Things (IOT) technology characteristics in railway transport system, this paper analyzed the integrated process. It designs railway information systemintegration scheme based on IOT in term of realizing railway informationsharing, so as to provide the theoretical and technical support for therealization of railway business and system information sharing and collaborative.

With the Internet development, Internet of Things(IOT) technology is widely used in the production. Combined with China'srailway system demand status quo for integration and sharing of resources andanalysis IOT key technology and operating principle, this paper proposes to constructthe railway information integration and sharing mode in IOT environment. Itincludes railway network system and railway information resources integrationand sharing platform based on IOT.

The development of the Internet of Things (IOT) has a significant impact on the development of railway information, dramatically improves the level of railway information. This paper will establish a sound and intelligent railway information platform based on the information construction achievements of railway transportation core business, with IOT technology and railway information platform architecture technology for technical support, and finally will achieve the goal of global control and management of information.

Distribution center plays an important role in the whole supply chain, and distribution center location decision is the core content of logistics systems analysis. Based on the analysis of the object of supply chain distribution center location, the main influence factors of the supply chain distribution center location were divided by using the ISM model, and a multi-grade hierarchical structure diagram of distribution center location was designed. By combining qualitative and quantitative methods, the hierarchical relationship of the main influence factors of supply chain distribution centers location is clarified.

Based on the analyses of the traffic characteristic of urban expressway ramps metering, a hybrid automaton model for expressway ramp metering is set up and switching control methods of hybrid system is proposed using the hybrid automata theory. Compared with fixed-time ramp metering, Alinea control algorithm and fuzzy control algorithm in different traffic flows, and simulated in the Paramics traffic software, the results show that the method of switching control based on hybrid automata theory is more effective than a single control algorithm.

According to the development of domestic and foreign Intelligent Railway, we integrate the needs of the mine railway system users, analyze internal data flow of the system, combine with the integration of the basic ideas and methodology, and use the model theory approach of model theory and system integration model. And then, we put forward mine railway intelligent transport digitizing systems integration model and design the overall framework of the mine railway intelligent transportation digitizing system

Considering the concept of service chain and the environment changes of supply chain logistics industry, the supply chain logistics based on service chain was proposed,on this basis of character, analysed the building of its service architecture and service information platform were deeply researched.

The process of remanufacture in supply chain management has already obtained some producers and managers' value, but actually has not obtained the corresponding attention and the research in the industrial engineering and the management science research area. At present, the market competition has not been a single competition among enterprises, it has gradually expanded to the entire supply chain or value chain. Therefore, from the point of view of supply chain management, this paper analyzed and compared the existing typical mode of production and organizational form in the field of re-manufacturing, Which concluded new features of mode of organization and management in re-manufacturing environment, and further combined with the problems existing in the field of re-manufacturing and the needs of the business to establish information system architecture, for the integrated information system in the remanufacture field to establish relevant technology base and advance some concrete measures to improve.

The paper proposes a space–time autoregressive integrated moving average (STARIMA) model to predict the traffic volume in urban areas. The methodological framework incorporates the historical traffic data and the spatial features of a road network. Moreover, the spatial characteristics in a way that reflects not only the distance but also the average travel speed on the links. In order to response the time-varying speed, six traffic modes are classified by level of service (LOS) which is updated in 5 minute interval. In the end, with the real traffic data in Beijing for experiments, the model achieves a very good accuracy on the 5 minute interval forecasting, it also provides a sufficient accuracy of 30 minute interval forecasting compared with ARIMA model.

OBJECTIVE To explore the correlation between syndrome differentiation and CT quantitative diagnosis of fatty liver. METHODS One hundred and thirteen cases of fatty liver verified clinically accepted plain CT scan after syndrome differentiation (syndrome of stagnation of liver qi and spleen deficiency, syndrome of intermingled phlegm and blood stasis, syndrome of phlegm-dampness blocking internally, syndrome of deficiency of liver and kidney, syndrome of interior dampness-heat), and then the CT values of the liver and the spleen were measured respectively. RESULTS It was shown that there was a correlation between the syndrome differentiation and the fatty infiltration in the liver. Significant differences of the distribution of fat and the ratio of liver-spleen were found among the five different syndromes (P

Background Increasing evidence indicates that the dysregulation of miRNAs expression is involved in the tumorigenesis by acting as tumor suppressors or oncogenes. However, no study investigates the function and mechanisms of miR-24 in nasopharyngeal carcinoma (NPC). Methods Quantitative RT-PCR, MTT, colony formation, soft-agar, wound healing, Transwell migration and invasion assays, and xenograft tumor growth and lung metastasis models were performed to test the expression levels and functions of miR-24 in NPC. Luciferase reporter assay, quantitative RT-PCR, Western blotting, and immunohistochemistry were used to identify and verify the target of miR-24. Results The results showed that MiR-24 was obviously downregulated in NPC cell lines and tissue samples (P

Objective:To evaluate the diagnostic value of multi-slice spiral CT (MSCT) for plasma cell mastitis. Methods: Radiographs of MSCT for forty-six patients with plasma cell mastitis diagnosed by pathological examination were reviewed. Results: The findings of MSCT of plasma cell mastitis could be divided into four types, including the inflammation type, the abscess type, the sinus and fistula type, and the mixed type, and each type had its radiographic characteristics. Conclusion: MSCT is helpful for diagnosing plasma cell mastitis and should be used as an examination of first choice for the patients.

Objects: to probe into the effects of PKCε on migration and paracrine functions of stem cells and potential molecular mechanisms. Methods: Bone Marrow mesenchymal stem cells (BMMSCs) were obtained from rat femur and passaged. mRNA and protein levels of capital proteins in PKCε signaling, SDF-1/CXCR4 axis and PI3K/AKT pathway in the MSCs in different conditions treating with PKC agonist, specific PKCε inhibitor, CXCR4 antagonist or PI3K inhibitor for 24 hours were analyzed by real-time PCR and western blot, and migration abilities were observed by migration assay in vitro and the changes of paracrine factors in different treatments were analyzed by protein clips assay. Results: the levels of p-JNK, p-P38MAPK, SDF-1, CXCR4, PI3K and p-AKT increased significantly after treating with PKC agonist (P < 0.05) and decreased obviously after treating with specific PKCε inhibitor. Migration ability and paracrine function of MSCs were enhanced in PMA group and attenuated in PKCε inhibitor group, and inhibiting activity of CXCR4 or PI3K attenuated the effects of PKCε, but not abolished completely. Conclusion: There was cross-talking between PKCε signaling and SDF-1/CXCR4 axis and PI3K/AKT pathway in signal transduction of MSCs. Activating PKCε could improve migration ability and paracrine function of MSCs partly at least independent of SDF-1/CXCR4 axis and PI3K/AKT pathway.

To compare the diagnostic value of gadolinium diethylenetriaminepenta-acetic acid (Gd-DTPA)-enhanced MRI with ultrasmall superparamagnetic iron oxide (USPIO)-enhanced MRI in differentiating reactive hyperplastic lymph nodes from metastatic lymph nodes in rabbit models.Reactive hyperplastic cervical lymph node model was established in 18 rabbits as hyperplasia group, and tumor-bearing lymph node model was established in another 18 rabbits as tumor group. For Gd-DTPA-enhanced MRI, T1WI and T2WI were performed on 9 animals of each model, and T1WI was acquired 80 seconds after administration of Gd-DTPA. For USPIO-enhanced MRI, T1WI, T2WI and T2*WI were performed on another 9 animals of each model before and 24 hours after administration of USPIO. MRI images were analyzed and correlated with surgical specimens and pathological results.In the tumor group, the sensitivity, specificity, positive predictive value, negative predictive value and accuracy of Gd-DTPA-enhanced MRI were 62.5%, 91.3%, 88.2%, 70.0% and 76.6%, respectively. The corresponding values of USPIO-enhanced MRI were 95.0%, 90.9%, 90.5%, 95.2% and 92.9%, respectively. The sensitivity and accuracy of USPIO-enhanced MRI differ significantly from those of Gd-DTPA-enhanced MRI. In the hyperplasia group, the accuracy of Gd-DTPA-enhanced MRI was 74.2%, while 87.1% for USPIO-enhanced MRI.USPIO-enhanced MRI has higher accuracy in diagnosing metastatic lymph nodes than Gd-DTPA-enhanced MRI.

To study possibility of dynamic monitoring tumor angiogenesis in vivo by 3D-CTA on a 64-row multidetector CT and 4D-CE-MRA on a 3T MR scanner; compare the advantages and faults of these two methods; and analyze the relationship between tumor angiogenesis and tumor growth.This study had been approved by the institutional animal care and use committee. Thirty New Zealand white rabbits with implanted VX2 tumors in the right thigh muscle were randomly divided into five groups according to survive time (n = 6). The rabbits were scanned by a 64-row multidetector CT and a 3T MR at 4, 7, 10, 13, 16 days after tumor implantation respectively, and then sacrificed to extract the tumor. The tumors' long diameters, short diameters and volume measurements derived from CT and MR imaging were compared with pathological data. The minimum tumor diameter and the number of new tumor blood vessels detectable by 3D-CTA and 4D-CE-MRA were also compared. The morphologic process of tumor angiogenesis was monitored and described.(1) The volume of tumors measured by CT imaging, MR imaging and pathological data had no significant difference (P0.05). (2) The minimum diameter of tumor vessels displayed on 3D-CTA and 4D-CE-MRA images was 0.68 mm +/- 0.07 mm and 0.85 mm +/- 0.12 mm respectively. The minimum diameter of tumor vessels displayed on 3D-CTA imaging was significantly smaller than 4D-CE-MRA imaging (t = -6.5075, P = 0.005). (3) The number of tumor vessels on 3D-CTA imaging and 4D-CE-MRA imaging had no significant difference at 4, 7, 10 days after tumor implantation. The number of tumor vessels on 3D-CTA images were significantly more than that on 4D-CE-MRA images at 13, 16 days after tumor implantation (all P0.01). (4) The morphologic process of tumor angiogenesis was demonstrated as sprouting from pre-existing blood vessel, forming lots of new vessels around tumor, and forming the immature vessels web with lots of tortuous, dilated, irregular vessels penetrating into the tumor at last.Volume CT and high magnet field MR can monitor tumor growth in vivo. 3D-CTA and 4D-CE-MRA can dynamicly monitor morphological changes of tumor angiogenesis in vivo. Of the two methods, 3D-CTA has better spatial resolution, but 4D-CE-MRA allows better temporal resolution of tumor angiogenesis monitoring.

It is shown that the consideration of three energy bands overlapping on the Fermi surface allows to calculate the superconducting transition temperature dependence on the chemical potential of YBa2Cu3O7-δ.

The braid group representation associated with the 10-dimensional representation of SU(4) is derived, based on the weight conservation and extended Kauffman diagrammatic technique. The corresponding trigonometric and rational solutions of the quantum Yang-Baxter equations are constructed in terms of the Yang-Baxterization procedure.

In this paper by employing the weight conservation and the diagrammatic techniques we solved the quantum Yang-Baxter equations. It is shown that the solutions associated with the 6 of are standard alone.

To prepare nanoparticles containing E1A gene and observe the efficiency and feasibility of transfecting E1A gene into human undifferentiated thyroid cancer cell line HTC/3. To examine the sensitivity of transgene cells to X-ray and X-ray-induced apoptosis in those cells.Nanoparticle-DNA complex was prepared with PLGA coating adenoviral early expression gene E1A, and the package efficiency, release progress in vitro, and size of the complex were determined. The nanoparticle-DNA was transfected into the HTC/3 cells. Lipofectamine was used to transfect E1A gene as a control. RT-PCR was used to examine E1A gene mRNA expression in the transfected cells. The survival ratio of HTC/3-E1A and control cells, and the growth inhibition ratio induced by different doses of X-ray in HTC/3-E1A cells were examined by MTT assay. The apoptosis in HTC/3-E1A cells induced by 2 Gy X-ray iradiation was examined by flow cytometry and DNA electrophoresis.The package efficiency, release progress in vitro, and size of the nanoparticle-DNA complex were 0.78%, 18 days, and 150-280 nm, respectively when transfected the plasmid at the same level, the nanoparticle group got more positive transgene cell clones than that in lipofectamine group, with a statistically significant difference (P0.05). RT-PCR showed that transgenic cells from both nanoparticle-DNA and lipofectamine groups had E1A gene mRNA expression. The HTC/3-E1A cells grew slowly, and their doubling time was prolongated (1.44 times in comparison with that in parental cells). According to IC50, the sensitivity of HTC/3-E1A cells to X-ray was improved 2.9 and 2.8 times, respectively, in comparison with that in HTC/3-Vect and HTC/3 cells. The ratio of subG0/G1 phase of HTC/3-E1A cells was significantly higher than that in HTC/3-Vect and HTC/3 cells (P0.01). The ratio of S phase of HTC/3-E1A cells was significantly lower than that in HTC/3-Vect and HTC/3 cells (P0.01). A typical DNA ladder pattern of apoptosis in HTC/3-E1A cells was observed by electrophoresis, but not found in HTC/3-Vect and HTC/3 cells.A nanoparticle-DNA complex has been successfully prepared, and it may carry a foreign gene into cells. The sensitivity of HTC/3-E1A cells to X-ray is significantly improved. Moreover, apoptosis is induced by x-ray in the E1A gene-transfected cells.

Objective. A combination of vasopressin and epinephrine may be more effective than epinephrine alone in cardiopulmonary resuscitation (CPR), but evidence is lacking to make clinical recommendations. This meta-analysis compares the efficacy of vasopressin and epinephrine used together versus epinephrine alone in cardiac arrest (CA). Methods. We searched MEDLINE and EMBASE for randomized trials comparing the efficacy of vasopressin and epinephrine versus epinephrine alone in adults with cardiac arrest. The primary outcome was the return of spontaneous circulation (ROSC) and the survival rate on admission and discharge .We also analyzed ROSC in subgroups of patients presenting with different arrest rhythms, such as asystole, pulseless electrical activity (PEA), ventricular fibrillation (VF). Results. We analyzed 6 randomized trials out of 485 articles. We did not find evidence supporting the superiority of vasopressin and epinephrine used in combination, except for the survival rate at 24h 2.99 95% CI(1.43,6.28). No evidence supports the conclusion that vasopressin combined with epinephrine is better than epinephrine alone for ROSC, even amongst subgroups of patients. Conclusion. This systematic review of the efficacy of vasopressin and epinephrine use found that its combined use is better for 24h survival rate but only in one study which included 122 patients. Further investigation will be needed to support the use of this combination for cardiac arrest management.