Search

Beta-glucan has been shown to have a beneficial effect on gastrointestinal health. This experiment was conducted to investigate the effects of β-glucan isolated from Agrobacterium sp. ZX09 on growth performance and intestinal health of weaning pigs. A total of 108 weaned pigs (21 d of age; 6.05 ± 0.36 kg) were randomly divided into 3 groups (6 pens/group; 6 pigs/pen), and the groups were each treated with the following diets: 1) basal diet, 2) basal diet supplemented with 20 mg/kg olaquindox, 3) basal diet supplemented with 200 mg/kg β-glucan, for 21 d. Compared with the control group, pigs fed with 200 mg/kg β-glucan had greaterBW, average daily gain and duodenal villus height to crypt depth ratio (P

Flexible perovskite solar cells (PVSCs) show promising applications in terrestrial power plant systems irrespective of curved surfaces, but still suffer from low efficiencies and poor stabilities. Here, we report a synergetic strategy of combining bromine (Br) and cetyltrimethylammonium chloride (CTAC) additives to passivate both bulk and surface defects, leading to improved performance and stability of flexible PVSCs. Incorporation of Br suppresses the formation of halide vacancies in the perovskite bulk, while CTAC further modifies the film surface with reduced defects and enhanced hydrophobicity features. As a result, flexible PVSCs with efficiencies over 18% are achieved with high reproducibility, compared to the control devices with efficiencies about 17%. Our flexible PVSCs present excellent ambient stability against moisture, keeping over 90% of the initial efficiency after exposure to 90% humidity for about 170 hours. Our work offers a facile and universal strategy for the fabrication of high performance and stable flexible PVSCs.

Yibo Geng,1,* Yuliang Wu,2,* Cheng Xu,1 Tian Li,1 Liwei Zhang1,3 1Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, People’s Republic of China; 2Department of Neurosurgery, Qilu Children’s Hospital, Shandong University, Jinan, Shandong, People’s Republic of China; 3China National Clinical Research Center for Neurological Disease, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Liwei ZhangDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 119 Nan Si Huan West Road, Fengtai District, Beijing 100070, People’s Republic of ChinaTel +86-15101181174Fax +86-010-59976611Email zhangliweittyy@163.comBackground: Numerous studies suggest that long non-coding RNAs (lncRNAs) participate in the biological process of diverse malignancies, including glioma. Although many differentially expressed lncRNAs have been identified in glioma, to our best knowledge, the role of LINC00662 and its potential underlying mechanism in glioma progression remains unclear. This study aimed to explore the function and regulatory network of LINC00662 in glioma.Methods: Expressions of LINC00662, miR-34a-5p and lectin mannose-binding 2-like (LMAN2L) in glioma tissues were analyzed using The Cancer Genome Atlas Program (TCGA) and the Chinese Glioma Genome Atlas (CGGA) databases. Colony formation, Celltiter-Glo and BrdU (5-bromo-2ʹ-deoxyuridine) incorporation assays were used to detect cell proliferation in vitro. Xenograft mouse models were established to determine cell proliferation in vivo. Transwell and wound healing assay was used to detect cell migration. In addition, epithelial–mesenchymal transition (EMT) markers were detected by Western blot. Annexin V and 7-AAD were used to stain apoptotic cells. Interactions between miR-34a-5p and LINC00662 or the 3′-UTR of LMAN2L were predicted and determined by bioinformatics analysis, luciferase reporter assay and RNA immunoprecipitation (RIP) assays.Results: High LINC00662 level predicted poor overall survival of glioma patients. Functional studies revealed that suppression of LINC00662 remarkably inhibited cell proliferation, clonogenicity and EMT pathway. Mechanistically, LINC00662 sponged miR-34a-5p to regulate LMAN2L expression. Furthermore, miR-34a-5p inhibitor reversed the anti-proliferation and anti-migration effect of LINC00662 knockdown, which could be rescued by downregulation of LMAN2L in glioma cells.Conclusion: Our study was the first to report that LINC00662 acted as a competing endogenous RNA (ceRNA) to regulate glioma progression by targeting miR-34a-5p/LMAN2L axis, providing a new therapeutic target for glioma.Keywords: long non-coding RNA, LINC00662, miR-34a-5p, LMAN2L, glioma, epithelial–mesenchymal transition

Ethanolamine(EA) often occurs at a relatively high concentration within the inflamed gut of IBD patients. To investigate the role of EA in colonic inflammation and host-microbiome dysbiosis, thirty-six ICR mice were treated with 3% DSS for a week to generate acute intestinal inflammation and then supplied with 0μM, 500μM (LowEA), and 3000 μM (HighEA) in drinking water for two weeks, after that,16s RNA sequencing was applied in characterizing the changes in colonic microbiota driven by different EA levels. An inflamed colonic organoid model via 3% DSS treatment was also established for further verification of these in vivo findings.EA significantly reduced proximal colonic crypt depth but increased distal colonic villus height in HighEA group. The protein and mRNA expression of occludin and Reg3β, BD1, BD2, and MUC2were significantly up-regulated in EA treated groups. EA decreased mucosal inflammation-related cytokines levels (IL1, IL6, IL17, TNFα, and INFγ) and increased the significantly increased concentration of sIgA. Serum aspartate aminotransferase and alanine aminotransferase were significantly down-regulated in the highEA group. EA increased the relative abundance of Blautia, Roseburia, Lactobacillus, Faecalibaculum, Candidatus_Saccharimonas, Alloprevotella, and Lachnoclostridum.and thus microbial metabolic pathways including Oxidative phosphorylation, Lipopolysaccharide biosynthesis, Arginine and proline metabolism, Folate biosynthesis, and Biotin metabolism were more abundant in LowEA group than those in control. EA up-regulated the protein or mRNA expression of TLR4/MyD88 in colonic tissues and the DSS-treated colonic organoid model. This study firstly demonstrated that ethanolamine in altering host-microbiome dysbiosis, which may provide new insights into the role of dietary lipids in IBD.ImportanceInflammatory bowel disease (IBD) affects ~3.1 million people in the USA and is increasing in incidence worldwide. IBD pathogenesis has been associated with gut microbiome dysbiosis characterized as a decrease in gut microbial diversity. Extensive works have demonstrated the roles of dietary fiber, short-chain fatty acids, and aromatic amino acids in altering the composition of gut microbiota to restore immune homeostasis and alleviate inflammation via diverse mechanisms in IBD. However, little is known about essential sphingolipids like ethanolamine (EA), an essential compound in the CDP-ethanolamine pathway for phosphatidylethanolamine (PE) in both intestinal cells and bacteria. PE synthesis deficiency can ultimately result in a loss of membrane integrity and metabolic disorders in IBD. Our results demonstrate that ethanolamine could improve colonic barrier functions and inflammatory immunoreactions via shifting microbiome dysbiosis, which provides new insights into the role of dietary lipids in IBD.

In the process of high sulfur coal mining, with coal gas outburst, a large amount of highly toxic flammable hydrogen sulfide (H2S) gas is produced which threats miner’s health and pollutes the environment seriously. It is shown that adding an oxidizing agent in alkaline absorption liquid can obviously increase the removal rate of H2S in mine air in the experiment results, and there’s no the hydrogen sulfide gas evolution after oscillation of the absorbing tail liquid, the removal efficiency is very remarkable. It is also shown that surfactant can reduce the surface tension of the absorption liquid in the research, accelerate the process of mass transfer between gas-liquid two phases, and improve the absorption efficiency.

Glioblastoma multiforme (GBM) is the most common and aggressive primary intracranial tumor. The tumor metastases outside the CNS are rare, so therapeutic experience with these types of tumors is limited. We present a case of a 20-year-old female with a history of left temporal anaplastic oligoastrocytoma, who was found to have biopsy proven metastases tothe lung. In April 2014, the patient presented with intermittent headache for 4 months. Physical examinations confirmed no obvious abnormalities. Computed tomography (CT) scan and magnetic resonance image (MRI) scan of the brain showed a large mass with ring-like enhancement in left temporal lobe. She underwent craniotomy to resect the intracranial tumor. Thepathological investigation showed the lesion to be a classical anaplastic oligoastrocytoma with focal necrosis and a Ki-67 labeling index of 10-30%. After operation, the patient received radiotherapy and chemotherapy. Two years later, the patient readmitted due to chest discomfort, a chest X-ray and CT showed a mass in the right lung. The lesion was confirmed to be a metastatic malignant glioma viapulmonary bronchoscopy biopsy. In conclusion, as the life expectancy is gradually increasing for GBM patients with newer therapies, the incidence of extracranial metastases may increase and this rare phenomenon may become more common and clinically relevant.

The significance and present situation of machine tool's thermal characteristics test technology is expatiated first. Aiming at certain three-axis vertical machine center's mechanical principal spindle, thermal characteristics test is done when principal spindle's temperature is rise and fall. Spindle thermal error analyser is used to test machine center's thermal errors in X, Y and Z directions and monitoring temperture of spindle. The spindle's temperture and thermal errors are presented and the test results are analyzed at last. The machine center's thermal information are mastered through test data and analysed results.

The adjoint system integrals for time free, optimal N-impulse transfer during a firing period in 5-phase selected are derived.

Helicases are enzymes that use ATP-driven motor force to unwind double-stranded DNA or RNA. Recently, increasing evidence demonstrates that some helicases also possess rewinding activity—in other words, they can anneal two complementary single-stranded nucleic acids. All five members of the human RecQ helicase family, helicase PIF1, mitochondrial helicase TWINKLE, and helicase/nuclease Dna2 have been shown to possess strand-annealing activity. Moreover, two recently identified helicases—HARP and AH2 have only ATP-dependent rewinding activity. These findings not only enhance our understanding of helicase enzymes but also establish the presence of a new type of protein: annealing helicases. This paper discusses what is known about these helicases, focusing on their biochemical activity to zip and unzip double-stranded DNA and/or RNA, their possible regulation mechanisms, and biological functions.

Polycystin-2 (PC2), encoded by PKD2, which is one of the genes whose mutations cause polycystic kidney disease, is abundantly produced in the apical domain of the syncytiotrophoblast (hST) of term human placenta. PC2, a TRP-type (TRPP2) non-selective cation channel, is present in primary cilia of renal epithelial cells, a microtubule-based ancillary structure with sensory function. The hST has abundant cytoskeletal structures, and actin filament dynamics regulate PC2 channel function in this epithelium. However, it is expected that the apical hST excludes microtubular structures. Here, we demonstrated by Western blot and immunocytochemical analyses that hST apical vesicles indeed contain microtubule structural components, including tubulin isoforms, acetylated alpha-tubulin, and the kinesin motor proteins KIF3A and KIF3B. PC2 and tubulin were substantially colocalized in hST vesicles. Treatment of hST vesicles with either the microtubular disrupter colchicine (15 microM) or the microtubular stabilizer paclitaxel (taxol, 15 microM) resulted in distinct patterns of microtubular re-organization and PC2 redistribution. We also observed that changes in microtubular dynamics regulate PC2 channel function. Addition of colchicine rapidly inhibited PC2 channel activity in lipid-bilayer reconstituted hST membranes. Addition of either tubulin and GTP, or taxol, however, stimulated PC2 channel activity in control hST membranes. Interestingly, we found that the kinesin motor protein KIF3A was capable of increasing PC2 channel activity in hST. We believe that the data are the first to provide a direct demonstration of a microtubular interaction with PC2 in the hST. This interaction thus plays an important regulatory role in the control of ion transport in the human placenta.

Objective To construct and identify the recombinant adenovirus containing SOCS3, detect SOCS3 expression level in HEK293 cells and provide valuable reference for gene therapy of myocarditis. Methods The SOCS3 gene was cloned by RT-PCR after total RNA extracted from peritoneal macrophage of rat, then subcloned with shuttle plasmid pAdtrack-CMV containing GFP reporter gene. PAdeasy-1 adenovirus system was used for co-transfecting shuttle plasmid and frame plasmid to Bj5183 cells. Reconstructed adenovirus was transfected to HEK293 cell after linearisation and mRNA and protein level of SOCS3 in the cell were detected. Results SOCS3 gene was cloned and recombinant adenovirus pAd-SOCS3-GFP was constructed successfully through pAdeasy-1 system. After linearisation, recombinant adenovirus was transfected to HEK293 cell and the mRNA and protein level of SOCS3 in the cell were both significantly increased. Conclusion Recombinant SOCS3 adenovirus has been constructed successfully and could be used for transfection in vivo or in vitro to treat inflammatory disease, for example, myocarditis.

Aims Atherosclerosis (AS) is an inflammatory disease induced by hypercholesterolemia. Previous studies indicated that suppressors of cytokine signalling (SOCS) protein could alleviate inflammation by inhibiting janus kinas/signal transducers and activators of transduction (JAK/STAT) pathway. The aim of this study is to investigate the expression trends of SOCSs protein in mice9s plaque/vessel and human peripheral blood mononuclear cell (PBMC) face to different level and different duration time of circulation cholesterol. Methods Cholesterol levels in blood, size and composition, expression of SOCS1 and SOCS3 of plaque of apoE null mice and C57BL6/j mice feed with high fat diet or chow during different periods were detected. In the non-coronary heart disease (non-CHD) population, cholesterol levels and mRNA of SOCS1 and SOCS3 in PBMC were also detected. Results The results showed that different expression patterns of SOCS1 and SOCS3 in the plaque of apoE null mice during increasing feeding duration with chow. The expression of SOCS3 presented an increasing trend (SOCS3/CD68 increased), but SOCS1 reached a peak in the middle of feeding period and returned to the initial level finally (SOCS1/CD68 decreased). In the high fat diet group, the SOCSs expression level increased correspondingly and the same trend occurred. The expression of SOCS1 and SOCS3 in mRNA and protein level of main aorta of mice was consistent with the trend above. The expression of SOCS1 and SOCS3 were barely observed in C57BL6/j mice with low circulation cholesterol level. In the non-coronary heart disease (non-CHD) population, the serum total cholesterol levels were positively correlated with SOCS3 mRNA in the PBMCs (r=0.433, p=0.012), but not relevant with SOCS1. Conclusion These results suggest that SOCSs family may play multiple roles in initial and development of AS, SOCS1 play an anti-inflammation role, but the effect of SOCS3 needs to be further researched on and reassessed in AS.