1. TREM‐1 deficiency attenuates the inflammatory responses in LPS‐induced murine endometritis
- Author
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Chen Jianguo, Mingxing Ding, Wen-ke Li, Li Han, Hongmei Zhu, and Zhuole Wang
- Subjects
Lipopolysaccharides ,Infertility ,Myeloid ,Necrosis ,Neutrophils ,lcsh:Biotechnology ,Bioengineering ,Stimulation ,Inflammation ,Applied Microbiology and Biotechnology ,Biochemistry ,Mice ,03 medical and health sciences ,lcsh:TP248.13-248.65 ,Polymorphonuclear Neutrophils ,medicine ,Animals ,Receptor ,Research Articles ,030304 developmental biology ,Mice, Knockout ,0303 health sciences ,030306 microbiology ,business.industry ,Uterus ,Bacterial Infections ,medicine.disease ,Triggering Receptor Expressed on Myeloid Cells-1 ,Disease Models, Animal ,medicine.anatomical_structure ,Immunology ,Cytokines ,Female ,Endometritis ,medicine.symptom ,business ,Research Article ,Biotechnology - Abstract
Summary Endometritis, which is usually caused by bacterial infection, is characterized by high levels of pro‐inflammatory cytokines and a high infertility rate. Triggering receptor expressed on myeloid cells‐1 (TREM‐1) has been recognized as a potent amplifier of inflammatory reactions. Studies have demonstrated reduced inflammatory responses and mortality rates of animals with bacterial infection due to the blocking of TREM‐1 expression. However, whether TREM‐1 deficiency could alleviate the inflammatory reaction in bacterial endometritis is still unclear. Here, TREM‐1 knock‐out (Trem‐1−/−) mice were used to inhibit TREM‐1 signalling to evaluate its role in inflammatory reactions after a highly pathogenic LPS infection in mice uteri. The results demonstrated that TREM‐1 deficiency attenuated the inflammation in mice uteri; markedly reduced the number of polymorphonuclear neutrophils; and suppressed interleukin‐1β (IL‐1β), IL‐6, and tumour necrosis factor‐α (TNF‐α) concentrations in serum as well as their production in inflamed uteri after LPS stimulation. Our results illustrate an anticipated pathogenic impact of TREM‐1 on endometritis during LPS infection and indicate that blocking of TREM‐1 in LPS‐induced endometritis holds considerable promise for blunting excessive inflammation.
- Published
- 2019