Search

Sodium (Na) vanadium (V) fluorophosphate $\mathrm{Na_xV_2(PO_4)_2F_3}$ (NVPF) is a highly attractive intercalation electrode material due to its high operation voltage, large capacity, and long cycle life. However, several issues limit the full utilization of NVPF's energy density: 1) the high voltage plateau associated with extracting the "third" Na ion in the reaction N$_1$VPF $\rightarrow$ VPF (~4.9 V vs Na/Na$^+$) appears above the electrochemical stability window of most practical electrolytes (~4.5 V); 2) a sudden drop in Na-ion diffusivity is observed near composition $\mathrm{Na_1V_2(PO_4)_2F_3}$. Therefore, it is important to investigate the potential substitution of V by other transition metals in NVPF derivatives, which can access the extraction of the third Na-ion. In this work, we investigate the partial substitution of V with molybdenum (Mo), niobium (Nb), or tungsten (W) in NVPF to improve its energy density. We examine the structural and electrochemical behaviors of $\mathrm{Na_xV_{2-y}Mo_y(PO_4)_2F_3}$, $\mathrm{Na_xV_{2-y}Nb_y(PO_4)_2F_3}$, and $\mathrm{Na_xW_{2}(PO_4)_2F_3}$ across the whole Na composition region of 0 $\leq$ x $\leq$ 4, and at various transition metal substitution levels, namely, y=0.5, 1.0, 1.5, 2.0 for Mo, and y=1.0, 2.0 for Nb. We find that partial substitution of 50% V by Mo in NVPF reduces the voltage plateau for extracting the third Na ion by 0.6 Volts, which enables further Na extraction from $\mathrm{Na_1Mo_{2}(PO_4)_2F_3}$ and increases the theoretical gravimetric capacity from ~128 to ~174 mAh/g. Analysis of the migration barriers for Na-ions in $\mathrm{Na_xVMo(PO_4)_2F_3}$ unveils improved kinetic properties over NVPF. The proposed $\mathrm{Na_xVMo(PO_4)_2F_3}$ material provides an optimal gravimetric energy density of ~577.3 Wh/kg versus ~507 Wh/kg for the pristine NVPF, which amounts to an increase of ~13.9%.

Internet gaming disorder (IGD) has been included in the fifth edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) as a condition warranting further study. Meanwhile, the World Health Organization (WHO) has included gaming disorder (both predominantly online and predominantly offline) in the ICD 11th revision. IGD represents a dramatic dysregulation of motivational circuits that is caused by a combination of exaggerated incentive salience and habit formation, reward defi cits and stress surfeits, and compromised executive function in three stages. Although significant progress has been made in understanding IGD and developing treatments, much is unknown about the pathophysiology of the disease, and rates of recurrence remain high. Exploring the neurobiological signature of IGD from new perspectives has the potential to transform current conceptualizations of the disease and sharpen the search for treatment targets.

Additional file 1.

Security, efficiency and availability are three key factors that affect the application of searchable encryption schemes in mobile cloud computing environments. In order to meet the above characteristics, this paper proposes a certificateless public key encryption with a keyword search (CLPEKS) scheme. In this scheme, a CLPEKS generation method and a Trapdoor generation method are designed to support multiple receivers to query. Based on the elliptic curve scalar multiplication, the efficiencies of encrypting keywords, generating Trapdoors, and testing are improved. By adding a random number factor to the Trapdoor generation, the scheme can resist the internal keyword guessing attacks. Under the random oracle model, it is proved that the scheme can resist keyword guessing attacks. Theoretical analyses and implementation show that the proposed scheme is more efficient than the existing schemes.

Objectives: To evaluate the feasibility, efficiency, and safety of the transradial approach (TRA) for cerebral angiography versus the transfemoral approach (TFA) in patients. Methods: In this trial, 2314 patients underwent cerebral angiography, with 1085 patients undergoing the procedure via radial access and 1229 via transfemoral access. The arterial puncture time, operation success rate, oppression time on puncture points, local vascular complication incidence (including bleeding, hematoma, and pseudoaneurysm), deep venous thrombosis of lower limbs (DVT), and bradycardia/hypotension were observed and compared between the two groups. Results: Of the patients who underwent cerebral angiography via radial access, the procedure was successful in 1070 patients; compared with 1219 patients with transfemoral access, there was no significant difference (P > 0.05) in the success rate or the arterial puncture time. Radial access patients were less likely to present with oppression time on puncture points, local vascular complications, DVT, and bradycardia/hypotension compared with femoral access patients. Conclusions: For patients undergoing cerebral angiography, radial and femoral approaches are both safe and effective. However, the lower rate of local vascular complications may be a reason to use the radial approach. Keywords: Cerebral angiography, Transradial approach, Transfemoral approach, Intervention

Additional file 4: Supplementary Table 4. Primary antibodies.

Additional file 1: Table S1. Association between lnc-RP11-536 K7.3 expression and clinicpathological factors in colon cancer TMA (n = 276).

Additional file 1: Supplementary Table 1. The clinicopathological characteristics and overall survival in ovarian cancer patients with different CTSL1 expression.

Additional file 3: Supplementary Table 3. Primer sequence.

Additional file 2. Supplementary Methods.

Additional file 2: Supplementary Table 2. sgRNA sequence.

Bis zum heutigen Tag ist die allgemeine Relativitätstheorie weithin als die relevante Theorie der Gravitation anerkannt und wurde durch viele Experimente bestätigt. Trotz dieses Erfolges liefert die allgemeine Relativitätstheorie keine Antwort auf viele offene Fragen, die mit Gravitation zusammenhängen. Das endgültige Schicksal scheinbarer Raumzeit-Singularitäten ist eine dieser offenen Fragen. Mögliche Lösungen für Raumzeit-Singularitäten werden in dieser Arbeit diskutiert. In diesen speziellen Lösungen werden Singularitäten durch Raumzeitdefekte ersetzt: Die Singularität des Schwarzen Loches wird durch einen Raumdefekt und die Urknall-Singularität wird durch einen Zeitdefekt ersetzt. In dieser Arbeit untersuchen wir bestimmte Effekte, die Raumzeitdefekte verursachen könnten. Wir berechnen die Geodäten der Raumzeitdefekte. Für den Raumdefekt diskutieren wir seine Linseneigenschaften und die entsprechende Bilderzeugung. Für den Zeitdefekt untersuchen wir die entsprechende nicht singuläre prallende Kosmologie. Wir präsentieren auch einige mögliche beobachtbare Effekte im Zusammenhang mit den Raumzeitdefekten.

Zhihong Chai,1,* Yufei Yang,2,3,* ZhongYi Gu,4,* Xianli Cai,1 Wenwei Ye,1 Lin Kong,1 Xiaoxiao Qiu,1 Lingxiao Ying,1 Ziliang Wang,2,3 Linyou Wang1 1Department of Gynecology and Department of Radiology, Taizhou Municipal Hospital Affiliated to Taizhou University School of Medicine, Taizhou 318000, People’s Republic of China; 2Department of Obstetrics and Gynecology, Xihua Hospital Affiliated to Shanghai Jiaotong University School Medicine, Shanghai 200092, People’s Republic of China; 3Department of Gynecological Oncology, Fudan University Shanghai Cancer Center, Shanghai 200032, People’s Republic of China; 4Department of Nephrology, Children’s Hospital of Fudan University, Shanghai 201102, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ziliang WangDepartment of Obstetrics and Gynecology, Xihua Hospital Affiliated to Shanghai Jiaotong University School Medicine, 1665 Kongjiang Road, Shanghai 200092, People’s Republic of ChinaEmail huf_zlwang@126.comLinyou WangDepartment of Radiology, Taizhou Municipal Hospital, 381 East Zhongshan Road, Taizhou 318000, People’s Republic of ChinaEmail wanglinyou2007@163.comPurpose: Capsid protein L2 is the minor capsid protein of human papillomavirus 16 (HPV16). Although L2-based vaccines were developed, the therapeutic effect of recombinant viral capsid protein L2 (rVL2) was still to be illustrated.Methods: We used glucose uptake and lactate production assay to verify the inhibitory effect of rVL2 on the glucose metabolism in cervical cancer cells. Secondly, we performed gene-chip assay, RT-PCR, and Western blot to determine the role of ITGB7/C/EBPβ signaling pathway in rVL2-mediated glucose metabolism in vitro. Finally, we used an animal model to verify the function of rVL2 in cervical cancer.Results: We found that rVL2 reduced glucose uptake and lactate production levels in cervical cancer cells, which caused the inhibition of cell proliferation. rVL2 decreased the expression levels of key metabolic enzymes, including GLUT1, LDHA, and ALDOA, to affect cell metabolism in cervical cancer cells by inhibiting ITGB7/C/EBPβ signaling pathway in vitro and in vivo.Conclusion: These results demonstrated the vital role of rVL2 in the glycolysis-induced cell growth and proliferation via suppressing ITGB7/C/EBPβ signaling axis.Keywords: recombinant viral capsid protein L2, rVL2, C/EBPβ, glucose metabolism, cervical cancer

The eastern margin of the Ordos Basin in the Linxing area has great potential for exploration of natural gas. However, the intrusion of the Zijinshan igneous pluton and associated hydrothermal activities have affected the sandstone reservoirs. Through the data analyses of rock thin sections, cathodoluminescence, X-ray diffraction, vitrinite reflectance, fluid inclusions, and reservoir physical property, this paper aims to clarify the influences of magmatic-hydrothermal activity and to explore the response characteristics of reservoir porosity and permeability. From the outer zone to the inner zone, the closer to the pluton, the closer the contact between the reservoir clastic particles, which confirms the compression caused by magma intrusion. The differences in feldspar dissolution and alteration, siliceous cementation, clay mineral content, vitrinite reflectivity, homogenization temperature and salinity of fluid inclusions, confirmed that magmatic-hydrothermal activity affected fluid properties and evolution of strata temperature. Combined with the vertical distribution characteristics of feldspar dissolution-alteration and the abnormal conversion of clay minerals, it is considered that the influence of hydrothermal fluid was limited to the reservoir under the thick mudstone of the Xiashihezi Formation. Magma intrusion causes the development of reservoir fractures, thus the permeability of the reservoir decreases from the inner zone to the outer zone, and the porosity presents the distribution pattern of “high-low-slightly high”. The reservoir porosity and permeability genetic model was established to provide references about favorable areas for oil and gas exploration and development in the Linxing area and areas with similar geological history.

Background Thromboembolic complications after stenting of intracranial aneurysms may be affected by antiplatelet administration. Aims This retrospective study aimed to assess the safety of intravenous tirofiban versus loading dose of oral clopidogrel for preventing thromboembolism in stent-assisted coiling of intracranial aneurysms. Methods From January 2006 to December 2013, 281 patients with cerebral aneurysms were treated with stent-coiling using two antiplatelet strategies in comparison: the initial strategy (a loading dose of ≥300 mg clopidogrel followed by dual antiplatelet, clopidogrel group) and the modified strategy (intravenous administration of tirofiban 8 µg/kg over 3 min followed by a maintenance dose of 0.1 µg/kg/min for 24 h, tirofiban group). The end points were rates of perioperative thromboembolic events and intracranial hemorrhages. Results Thromboembolic events were observed more often in the clopidogrel group (13/120 aneurysms, 10.83%) than the tirofiban group (6/178 aneurysms, 3.37%; P = 0.010), with no increase in the rate of intracranial hemorrhages ( P = 0.164). In the ruptured subgroups, thromboembolic events were significantly fewer in the tirofiban subgroup (5/128, 3.91%) compared with the clopidogrel subgroup (7/53, 13.21%; P = 0.043) with no increase in the rate of hemorrhage ( P = 0.360). Conclusions Intravenous administration of tirofiban is safe in intracranial aneurysms treated with stent-assisted coiling.

Biomass, a nearly carbon-neutral energy resource, can reduce greenhouse gas emission and replace fossil fuels, but it is characterized by heterogeneity, high moisture content, low bulk density, low calorific value, pliability and hygroscopic nature, all of which challenge its utilization. Torrefaction, a thermal pretreatment method, is capable of modifying the physical-chemical properties of biomass and enhancing its calorific value. Slot-rectangular spouted beds (SRSBs) can effectively handle biomass particles and offer a promising way to overcome the scale-up challenge of conventional spouted beds. This study explored the potential application of SRSBs to biomass torrefaction. Solids mixing in a dual-compartment slot-rectangular spouted bed (DSRSB) was first studied to address the scale-up issue, while also providing fundamental information needed for the design and operation of a DSRSB reactor. SRSB and DSRSB reactors were developed for torrefying sawdust with the semi-batch operation. Hydrodynamics of SRSB and DSRSB, torrefaction performance, torrefied product properties and torrefied product pyrolysis were subsequently investigated. Temperature, biomass feed rate, sawdust particle size and oxygen concentration influenced torrefaction performance and torrefied product properties. Temperature was found to be the most important factor. Biomass torrefaction performed better in the DSRSB than in the SRSB. Higher temperature, lower biomass feed rate, larger sawdust particle size and greater oxygen concentration all led to increased weight loss and decreased energy yield of sawdust, and produced torrefied sawdust with higher HHV, greater atomic carbon content, lower atomic hydrogen and oxygen contents, less volatile matter, greater fixed carbon and less hemicellulose. Higher temperature and greater oxygen concentration were very helpful to produce more torrefied sawdust captured by a cyclone. Performance of oxidative torrefaction was similar to that of non-oxidative torrefaction. The effect of oxygen concentration was more significant at a higher temperature. Torrefied sawdust underwent size reduction during torrefaction, with smoother and cleaner surfaces compared to raw sawdust. The activation energy for non-oxidatively torrefied sawdust was higher than for oxidatively torrefied sawdust, which was in turn greater than that of raw sawdust. Sawdust particle size affected the pressure drop across the reactor.

Cancer cells harbor lower energy consumption after rounds of anticancer drugs, but the underlying mechanism remains unclear. In this study, we investigated metabolic alterations in cancer cells exposed to cisplatin. The present study exhibited cisplatin, known as a chemotherapeutic agent interacting with DNA, also acted as an anti-metabolic agent. We found that glycolysis levels of breast and cervical cancer cells were reduced after cisplatin treatment, resulting in cells growth and proliferation inhibition. We demonstrated that cisplatin suppressed glycolysis-related proteins expression, including glucose transporter 1 (GLUT1), glucose transporter 4 (GLUT4) and lactate dehydrogenase B (LDHB), through down-regulating integrin β5 (ITGB5)/focal adhesion kinase (FAK) signaling pathway. ITGB5 overexpression rescued cisplatin-induced inhibition of cancer cell glycolysis, growth and proliferation. Conclusively, we reveal a novel insight into cisplatin-induced anticancer mechanism, suggesting alternative strategies to the current therapeutic approaches of targeting ITGB5, as well as of a combination of cisplatin with glucose up-regulation chemotherapeutic agents to enhance anticancer effect.

To monitor the abuse of Norfloxacin (NFLX), developing a monoclonal antibody (mAb) based enzyme linked immunosorbent assay (ELISA) kit is our future goal. For this purpose, Hybridomas were prepared by fusing NS0 mouse myeloma cells with splenocytes isolated from immunized BALB/c mouse. Noncompetitive and competitive indirect ELISA was employed to screen positive cell clones. Caprylic acid ammonium sulphate (CAAP) method was used to purify NFLX mAb and the Batty saturation method was used to determine the affinity constant (Ka). Finally, six hybridoma cell lines named N1-A2, N2-B3, N2-B5, N3-C6, N4-D5, and N4-D8 were screen out, their corresponding mAbs were of the IgG1 isotype with k light chain, and the Kas of all mAbs were between 3.2×109 and 2.6×1010 L/mol. Based on the square matrix titration, two representative icELISA curves were established. The dynamic range for N3-C6 in assay buffer was from 0.003 to 38 ng/mL, with LOD and IC50 value of 0.002 ng/mL and 0.16 ng/mL, respectively. As to N2-B5, it was from 0.004 to 26 ng/mL, with LOD and IC50 value of 0.002 and 0.22 ng/mL, respectively. Except for a high cross-reactivity (CR) to Pefloxacin (33.6%) and Lomefloxacin (21.8%), this mAb originated from N3-C6 exhibited negligible CR values to other chemicals. Therefore, the established hybridomas put a potential for the development of a rapid test kit and provide an alternative method for the detection of NFLX residues in food producing animals.

An indirect competitive ELISA (icELISA) method for multiresidue determination of Fluoroquinolones (FQs) residues in fish samples has been developed. For this purpose, Sarafloxacin (SAR) was employed to synthesize the immunogen and coating antigen through EDC conjugation method, and cell fusion technology was used to produce anti-SAR monoclonal antiobdy. Based on the square matrix titration, an icELISA method was established. The dynamic range for Sarafloxacin in assay buffer was from 0.004 to 18 ng/mL, with LOD and IC 50 value of 0.002 ng/mL and 0.32 ng/mL, respectively. After optimization, the physiological pH (7.4) was selected for the immunoassays, and this assay could tolerate up to 10% acetonitrile. The results of this assay showed a high cross- reactivity to Difloxacin (85.5%), Norfloxacin (61.7%), and Pefloxacin (34.8%). Under the 10-fold dilution in authentic fish samples, the regression curve equations for Sarafloxacin, Difloxacin, Norfloxacin and Pefloxacin were y = 1.0114x - 0.4003, R 2 = 0.9901; y = 0.9782x + 0.2754, R 2 = 0.9807; y = 0.9892x +0.0489, R 2 = 0.9843; and y = 0.9797x +0.8017, R 2 = 0.9844, respectively. The results suggest this immunoassay can be used for simultaneous detecting four kinds of FQs in fish samples.

In traditional multi-model algorithm, the models' parameters are set in advance without any changes in the following calculating steps, sometimes these parameters areinitialized randomly. In order to set the parameters more accurate, algorithm based on genetic algorithm (GA) is proposed. Combined with recursive interacting multi-model (RIMM) algorithm proposed by Leigh A. Johnston, the optimal searching ability of GA is used to identify the best model parameters in one searching period. The proposed method is applied to inertial navigation system (INS)/celestial navigation system(CNS)/GPS integrated navigation for missile. The experiment results exhibit satisfactory precision and searching velocity. By determining the models' parameters of the whole filtering adaptively, it reaches a balance between two incompatible goals: “rapid velocity” and “high precision”.

The attitude of the spacecraft can be obtained with accuracy by using star sensors. Its key technology is how to identify stellar maps. Triangle algorithm is used most widely in the current star map identification algorithms. In the field of physical geography modelling, Delaunay triangulation cutting algorithm has been widely used because of its important characteristic; the result of this triangulation cutting method is unique from a random set of points. This triangulation algorithm is first applied to identify star map for its particular character. In final, the proposed method is applied to simulation; the results show that compared with the current star map identification algorithms, the algorithm presented in this paper has the advantage of real-time properties and robustness. It needs a smaller database of guidance stars and matches more rapidly.

First, the conditions for GM(1,1) modeling are analyzed, and it revealed that the whole trend of the class ratio sequence and the whole concave‐convex of data sequence both affect the precision of the model. Secondly, the action of translation transformation on the features of data sequence is studied theoretically, and the satisfied interval of translation values is derived. Finally, an effective numerical method is presented which is illustrated by a medical example.

Nowadays about 0.5 percent of people in the world are blind. In another word, about 30 million people are unfortunately blind in the world because of congenital or acquired defects. Without the basic visual ability, blind people cannot or difficult to walk to a certain destination by themselves. Under this situation, we considered that if blind people can find the way with guide in a certain occasion, the guide doesn't come from a person but an intelligent device with RFID system. This RFID system is an indoor route-guide system for blind people to clearly find the way in a building without others' help. As the most useful and powerful guide system, it is designed to base on Android platform and the XT200 which is a multifunctional Bluetooth RFID device. The hardware consists of several parts which should be installed at first. This report aims to realize the software part of this system, which means an operation system with a driver that can identify the near tags by Bluetooth. When identification is done, the device will provide a specific route to destination and tell the user by voice. This navigation system may not solve the visual problems of visual disabilities, but it will be very helpful to provide convenience in some way. In longer term, this system may be proved quite useful, for outdoor guiding or automobile navigation. Master of Science (Communications Engineering)

Alumina ceramic with enhanced dielectric property was reported in this paper, which indicates its microwave application. Commercial A1203 powder and sintering aids including MgO were utilized to fabricate alumina ceramic substrate. Liquid deposit was introduced to wrap the surface of A1203 powder with MgO additives uniformly. The prepared composite powder was shaped by tape casting and sintered by two-step sintering to ensure density and suitable microstructure. It is shown that MgO addition restrains gain growing and decreases porosity in scanning electron micrograph. MgA1204 spinel, formed by MgO was detected by XRD. The dielectric loss of the alumina ceramic substrate is 6.84 × 10−4.

To meet the employment situation in modern society, and expand career opportunity, college students should have solid theoretical foundation and excellent practical skills, and can solve the practical problems in society or related profession. In the process of cultivating high-quality talents in veterinary medicine, the following improvements should be considered: fast the practical teaching reform, establish the rules of practical teaching, strengthen the teachers' practical skills, increase the amounts of practical base in or off-school, refresh the practical teaching curriculum, stress on training of applied talents, and pay great attention to the needs of social development.

Artificial hapten Cd-ITCBE was synthesized using isotrhiocyano benzyl-EDTA(ITCBE) to chelate Cd2+. The isothiocyanate method was used to conjugate Cd-ITCBE to bovine serum albumin (BSA) and artificial immunogen Cd-ITCBE-BSA was obtained, the detection antigen Cd-ITCBEOVA was obtained in the same way. Ultraviolet (UV), sodiumdodecyl sulfate polyacrylamide gel electropheresis (SDS-PAGE)and inductively coupled plasma atomic emission spectroscopy(ICP-AES) were used to identify Cd-ITCBE-BSA and Cd-ITCBE-OVA. Balb/C mice were immunized with Cd-ITCBEBSA and hybridoma lines that secreted Cd2+ mAb were generated by cell fusion and then screened by indirect ELISA, blocking ELISA. The Cd2+-Kit was established with Cd2+ mAb and its traits were tested. The Results showed that Cd-ITCBEBSA was synthesized successfully and its concentration of BSA and Cd2+ was 7.1 mg/mL and 191.7 ¦Ig/mL respectively. Four hybridoma lines of 1A3, 2B7, 2E10, 4F3 were screened out and the best one was 2E10 and its affinity constant(Ka) was7.58iA108 L/moL. The calibration curve of Cd2+-Kit was typical sigmoid curve fitted to the four parameter logistic equation with the IC50 of 16.3 ¦Ig/L, the detection limit of 3 ¦Ig/L. The recoveries of Cd2+ spiked in water were 89.28%, in soil were94.28% and in feed were 92.88% and the coefficient variation were below 15%. The specificity of Cd2+-Kit had 96.5% cross reactivity with Hg2+ and little or no cross-reactivity with other compounds. The validity of Cd2+-Kit in 4 iae was above 180 d.

This paper described the propagation and cavity characteristics of substrate integrated waveguide (SIW). Authors designed a SIW filter based LTCC with HFSS and simulated the transmission behavior. Then prototype SIW filters were fabricated from our 8" LTCC line. The measurement results with VNA matched well the simulation data. After analyzed the reason for the difference between the simulation and measured curve author conclude that this is a new way to integrate passive component in LTCC.

Grey relational analysis is a kind of effective tool to make system analysis. Grey superiority analysis is a very important step to make relational analysis. Up to now the research about superiority analysis is relatively weak. First the existing basic theory and methods of grey relational analysis and grey superiority analysis are briefly introduced, and their defects are pointed out. Then a new algorithm, that is, the method of ordering with ideal point, to determine the grey relational ordering is presented. Moreover combined with clustering technique, a new concept of grey relational clustered ordering is put forward, and its algorithm is given. It is revealed that grey relational clustered ordering may reflect the difference and similarity of the influences of factors or characteristics, and use limited data information more effectively. The validity of the new methods is verified via an example in practice.

The testing method of the fitting accuracy of grey dynamic models is mainly studied in this paper. First the existing testing methods, e.g., residuals testing and posteriori deviation testing, are briefly introduced and evaluated, and the merits and defects of the existing testing methods are pointed out, e.g., the existing testing method indeed do not consider the quality for grey models to fit for the trend of data sequences. Then the features, which a kind of testing method for the fitting accuracy of grey model should possess, are analyzed. At last, based on the specific features of grey models, a new kind of testing method for the fitting accuracy of grey dynamic model is designed, that is, trend testing, which may reflect the following aspects: (1) the extent of fitting the whole trend of data sequences; (2) the extent of fitting each point of data sequences; (3) the comparability of results among different problems.

An indirect competitive ELISA has been developed for the determination of Norfloxacin (NFLX) residue in chicken liver. For this reason, carbodiimide active ester method was employed to synthesize the artificial antigen of NFLXBSA, and coating antigen of NFLX-OVA, and rabbits were used to produce the anti-NFLX polyclonal antibody. Based on the square matrix titration, an icELISA method was developed for the quantitative detection of NFLX. The dynamic range was from 0.006 to 38 ng/mL, with LOD and IC50 value of 0.004 ng/mL and 0.48 ng/mL, respectively. Except for a high cross-reactivity to Ciprofloxacin (38%) and Sarafloxacin (33%), negligible cross-reactivity to the other compounds was observed. After optimization, 10-fold dilution in chicken liver extract gave an inhibition curve almost the same as that in PBS buffer. When spiked in liver extract at 8, 20, and 50 ng/mL, the recovery rate was 90- 117.8% for intra-assay, and 97.5-114.4% for inter-assay, respectively. The relative standard deviation ranged from 5.6-9.2% for intra-assay and 5.9-11% for inter-assay. Therefore, the developed icELISA method could be used for the routing screening of NFLX residues in chicken edible tissues.