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Background Circular RNAs (circRNAs) have been shown to play a crucial role in tumorigenesis. In this study, we investigated the function of hsa_circ_0137008 and its underlying molecular mechanism in colorectal cancer (CRC). Methods Gene expression was conducted by quantitative real-time PCR or western blot. Functional experiments were performed by cell count kit-8, colony formation assay, wound healing, and transwell assays. Luciferase reporter assay and RNA pull-down assay were performed to investigate the molecular mechanism of hsa_circ_0137008 in CRC. In addition, the xenograft tumor model was applied to determine the role of hsa_circ_0137008 in vivo. Results Downregulation of hsa_circ_0137008 was observed in CRC tissues and cell lines. Functionally, overexpression of hsa_circ_0137008 inhibited the proliferation of CRC cells, as indicated by the inhibition of proliferative protein expression (Ki67 and PCNA), reduced cell viability and colony formation ability. Upregulation of hsa_circ_0137008 suppressed the migration, invasion, and epithelial to mesenchymal transition (EMT) of CRC cells. Mechanically, hsa_circ_0137008 negatively regulated the expression of microRNA-338-5p (miR-338-5p). Furthermore, hsa_circ_0137008 abated the miR-338-5p mediated promotion on CRC cell progression. Tumor suppressive function of hsa_circ_0137008 was validated in vivo. Conclusion These findings highlighted the fact that overexpression of hsa_circ_0137008 inhibited the progression of CRC via sponging miR-338-5p, suggesting that hsa_circ_0137008/miR-338-5p axis is a principal regulator of CRC tumorigenesis.

This paper provides a comprehensive overview of cryptocurrencies, including the origin of cryptocurrencies, how cryptocurrencies operate, and the current situation of cryptocurrencies. In addition, we also provide the performance comparison of major cryptocurrencies with the performance of the stock market indexes. All the cryptocurrencies exhibit higher average returns and volatility than the stock market indexes, which appeals to risk-taking investors. We then perform additional analysis on the determinants of cryptocurrencies returns. We show that major fundamental variables are less likely to affect the returns of cryptocurrencies except for the S&P 500 index returns and the exchange rates between U.S. dollars and Euros.

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Huafu Ye, Qiaozhi Jin, Xiaoqiong Wang, Yong Li E.N.T. Department, Taizhou Municipal Hospital, Taizhou City, Zhejiang Province 318000, People’s Republic of ChinaCorrespondence: Yong LiE.N.T. Department, Taizhou State Hospital of Zhejiang Province, No. 381 Zhongshan East Road, Taizhou City 318000, Zhejiang Province, People’s Republic of ChinaTel +86 15168647760Email prqinmo271026@126.comBackground/Aims: miR-802 plays a key role in cancer progression and development. The purpose of this work is to investigate the functional role of miR-802 in laryngeal cancer and to elucidate the function of miR-802 and cAMP-regulated phosphoprotein 19 (ARPP19) on laryngeal cancer.Methods: RT-qPCR was applied to study the expression level of ARPP19 and miR-802 in the laryngeal carcinoma cell lines and tissues. CCK-8, colony formation, flow cytometry (FACS) assay were used to study the effect of ARPP19 and miR-802 on apoptosis, proliferation, and cell cycle of laryngeal carcinoma cells. Target gene prediction and luciferase reporter gene assay were applied to identify target gene of miR-802. The transcriptional mRNA and protein expression levels of ARPP19 were measured by RT-qPCR or Western blotting.Results: miR-802 was down-regulated in laryngeal carcinoma cell lines and tissues. Laryngeal cancer cells transfected by miR-802 mimic were significantly inhibited in the terms of cell colony formation and proliferation. Furthermore, miR-802 can inhibit the expression level of ARPP19 by directly targeting the 3ʹ untranslated region (3ʹ-UTR) of ARPP19. Overexpression of the ARPP19 gene can reverse the suppressive effect of miR-802 on laryngeal cancer cells.Conclusion: miR-802 can exert tumor suppressor effects in laryngeal carcinoma by targeting ARPP19, indicating that miR-802 protein may play a role of potential therapeutic target for clinical laryngeal cancer.Keywords: miR-802, laryngeal carcinoma, ARPP19, proliferation, cell cycle

An effective evaluation on fracture network forming capacity is key to the whole process of shale gas exploration. At present, neither a clear standard nor a generally accepted evaluation method exist. In this study, a novel “Soundless Cracking Agent (SCA) fracturing evaluation method” was developed. The fractures were characterized quantitatively using fractal dimension of the trace on the core surface and areal density. Acoustic emission (AE) location was used for dynamic monitoring and analysis. The results show that the fractal dimension can be used for quantitative evaluation of complexity of fracture network. The higher the rock hardness, the smaller the fracture density after fracturing is; the higher the brittleness, the larger the fracture density after fracturing is. The development degree of natural fracture systems and sedimentary bedding is a key factor to control the propagating morphology of fractures. The number of AE events for sandstones with low clay content (

In this paper, an optimization technique combining non-conformal integral equation domain decomposition method (IE-DDM) and particle swarm optimization (PSO) is presented for the design of antenna on platform. The antenna and the platform can be decomposed into different closed sub-domains according to design requirements and the DDM is used to compute the scattering from antenna on platform. Meanwhile PSO is used to find the best antenna location. Numerical example is presented to demonstrate the efficiency of the present method.

In order to denoise shipborne radar (9GHz pulse radar) echo signal in distortion-free, smooth, and less loss, a new threshold and shrinkage function are proposed in this paper based on the Multi-analysis wavelet threshold denosing. The denoising effects of different shrinkage function for shipborne radar echo signal were compared. The results of experiment show that better SNR and similarity and smoothness and less loss can be reached by using the proposed function to shipborne radar echo signal.