Search

Statement of problem A consensus on the clinical performance of implant-supported removable partial dentures (ISRPDs) is lacking. Purpose The purpose of this systematic review and meta-analysis was to evaluate the clinical performance of ISRPDs in terms of the implant survival rates, marginal bone loss, and patient-reported outcome measures (PROMs). Material and methods Four electronic databases (MEDLINE/PubMed, EMBASE, Web of Science, and Cochrane Library) were independently searched by 2 reviewers for articles published up to December 2020. A single-arm meta-analysis was performed to evaluate the implant survival rates and marginal bone loss by using the R program. The Cochrane collaboration tool was used to evaluate the risk of bias of randomized controlled trials (RCTs), and the Newcastle-Ottawa scale for non-RCT studies. Results Sixteen studies were included, with a total of 334 participants and a mean age of 58 years. The participants received a total of 581 dental implants, 475 conventional and 106 mini-implants. All included studies reported implant survival rate above 90% (range: 92% to 100%). Meta-analysis indicated a high proportion of implant survival rates of 3% (95% confidence interval [CI]: 2% to 5%) and a low mean raw score of marginal bone loss 0.98 mm (CI: 0.61 to 1.36 mm). Compared with conventional RPDs, improved patient quality of life and satisfaction were reported by studies that evaluated ISRPDs. RCT studies exhibited a low risk of bias for most domains, while most non-RCT studies were classified as good quality. Conclusions ISRPDs exhibited high implant survival rates and acceptable bone loss with improvement in the quality of life and satisfaction of patients when compared with conventional RPDs. Therefore, they can be considered suitable for the rehabilitation of partially edentulous patients.

Selective clamping during robot-assisted partial nephrectomy (RAPN) may reduce ischemia-related functional impairment. The intraoperative use of 3D-virtual models (3DVMs) can improve surgical planning, resulting in a greater success rate for selective clamping. Our goal is to introduce a new generation of 3DVMs, which consider the perfusion volumes of the kidney. Patients listed for RAPN from 2021 to 2022 were recruited. A selective clamping strategy was designed and intraoperatively performed based on the specifically generated 3DVMs. The effectiveness of selective clamping was evaluated using near-infrared-fluorescence imaging (NIRF) and 3DVM. Perfusion areas extensions were compared, and relevant preoperative characteristics were analyzed. In 61 of 80 (76.25%) cases, selective clamping was performed. The concordance between the 3DVM areas and the NIRF-enhanced areas was verified (k = 0.91). According to the distribution of perfused areas crossing the tumor, there were one, two, three, four, and five crossing areas, with relative perfusion rates of 13.75%, 35%, 32.5%, 13.75%, and 5%, respectively. Lesion diameter and mesorenal location were the only factors related to a higher number (>3) of perfusion volumes crossing the lesion. The implementation of mathematical algorithms to 3DVMs allows for precise estimation of the perfusion zone of each arterial branch feeding the organ, leading to the performance of safe and effective pedicle management planning.

Background: Our study aims to assess the feasibility and the reproducibility of fluorescent confocal microscopy (FCM) real-time assessment of urethral and ureteral margins during open radical cystectomy (ORC) for bladder cancer (BCa). Methods: From May 2020 to January 2022, 46 patients underwent ORC with intraoperative FCM evaluation. Each specimen was intraoperatively stained for histopathological analysis using FCM, analyzed as a frozen section (FSA), and sent for traditional H&E examination. Sensitivity, specificity, positive predictive value (PPV), and the negative predictive value (NPV) of FCM and FSA were assessed and compared with H&E for urethral and ureteral margins separately. Results: The agreement was evaluated through Cohen’s κ statistic. Urethral diagnostic agreement between FCM and FSA showed a κ = 0.776 (p < 0.001), while between FCM and H&E, the agreement was κ = 0.691 (p < 0.001). With regard to ureteral margins, an overall agreement of κ = 0.712 (p < 0.001) between FCM and FSA and of κ = 0.481 (p < 0.001) between FCM and H&E was found. Conclusions: FCM proved to be a safe, feasible, and reproducible method for the intraoperative assessment of urethral and ureteral margins during ORC. Compared to standard FSA, FCM showed adequate diagnostic performance in detecting urethral and ureteral malignant involvement.

During the Hellenistic period and under the growing influence of the art of painting, the polychromy of Greek terracotta figurines focused not only on an elaborate rendering of color, but also on the interplay of light and shadow. Some of the best-preserved examples clearly show the subtlety of such pictorial effects. Among them is a statuette of a standing Maenad, held in the collections of the National Archaeological Museum in Athens (inv. 5000). Dating back to 150–100 BCE, it is a high-quality testament to the sculptural, as well as pictorial, coroplastic production in the workshops of Myrina (Eolide, Turkey). Combining multi-scale examination, multi-spectral imaging and non-invasive spectroscopic investigations (XRF, FTIR, FORS), a scientific study of the artefact was carried out within the framework of the Pilina project, a collaborative research program between the Louvre, the C2RMF, the National Archaeological Museum and the French School in Athens. This article presents the main results of the study by discussing the color scheme, identification of some pigments and colorants (clays of the kaolinite group, ochres, cinnabar, Egyptian blue, an anthraquinone of plant origin, likely madder, gold leaf), and painting techniques aiming at achieving chiaroscuro effects.

Objetivo: Relatar a eficácia solução hipertônica do Cloreto de Sódio (NaCl 20%) em granulomas periestomais de traqueostomias após decanulação. Método: Trata-se de dois relatos de casos com amostragem por conveniência e não probabilística, que avaliou o uso de solução hipertônica em granulomas periestomais de traqueostomias, que passaram pelo processo de decanulação da traqueostomia e que estiveram internados durante o tratamento do granuloma. Resultados: A pesquisa identificou como benefício do tratamento com NaCl 20% uma evolução satisfatória dos granulomas, um pequeno número de aplicações necessárias, o baixo custo da solução e ausência de efeitos adversos constatados. Conclusão: A pesquisa constatou um resultado positivo no uso de NaCl 20% em granulomas periestomais de traqueostomia, pois propiciaram a redução total dos granulomas e com isso permitir a cicatrização completa das lesões.

Gli studi volti all'analisi del disagio psicologico delle pazienti con tumore al seno sono sempre più frequenti. Tuttavia, i correlati psicologici e psicosociali dei sintomi ansioso-depressivi (SAD) nel carcinoma mammario richiedono ulteriori approfondimenti, alla luce dell'eterogeneità dei protocolli impiegati per l'assessment psiconcologico. La presente rassegna sistematica, condotta mediante la banca dati Scopus, si è posta il duplice obiettivo di identificare e classificare i principali correlati dei SAD nelle pazienti oncologiche, e indagare il ruolo svolto da tali correlati verso l'insorgenza, il mantenimento e/o la severità dei SAD. La rassegna, basata su criteri di eleggibilità prestabiliti e procedure sistematiche di screening e valutazione, ha preso in esame 64 studi osservazionali (trasversali e longitudinali) pubblicati nel decennio 2012-2022. Dagli studi è emerso un legame fra SAD (rilevati con strumenti di screening), ansia e/o depressione (valutati con questionari specifici), e tre macro-categorie di costrutti: 1. altri sintomi di disagio psicologico, positivamente correlati ai SAD; 2. il capitale psicologico e psicosociale delle pazienti, inversamente correlato ai SAD; 3. ulteriori fattori che influenzano la salute e l'aderenza terapeutica. Le implicazioni della presente rassegna della let-teratura si realizzano nell'opportunità di adottare protocolli valutativi e di intervento integrati, orientando studi futuri verso un'analisi ed una maggiore comprensione della variabilità delle procedure di assessment e delle diverse risposte delle pazienti al disagio cancro-correlato.

This paper presents the message passing interface (MPI)-based parallelization of the three-dimensional hydrodynamic model SHYFEM (System of HydrodYnamic Finite Element Modules). The original sequential version of the code was parallelized in order to reduce the execution time of high-resolution configurations using state-of-the-art high-performance computing (HPC) systems. A distributed memory approach was used, based on the MPI. Optimized numerical libraries were used to partition the unstructured grid (with a focus on load balancing) and to solve the sparse linear system of equations in parallel in the case of semi-to-fully implicit time stepping. The parallel implementation of the model was validated by comparing the outputs with those obtained from the sequential version. The performance assessment demonstrates a good level of scalability with a realistic configuration used as benchmark.

The probing of software by security testers to detect possible vulnerabilities is of primary importance to prevent attackers from exploiting these flaws, which can, besides other things, compromise the application and permit access to sensitive data. Traditionally, these security testers are static, dynamic and symbolic analyzers. These tools, in general, present a compromise between precision and complexity. A recent alternative to these methods is the use of machine learning models, which can avoid this tradeoff altogether, rendering precise predictions with acceptable complexity. Recently, many models have been proposed in the literature, drawing much inspiration by the recent advances in the field of Natural Language Processing. Despite this recent exploration of machine learning methods, there is little study in the realm of interpreting these models' output, with the goal of achieving not only the classification of whether a snippet of code is vulnerable or not, but to be capable of pointing in the code where the model believes the vulnerability is located. In this work, we fine-tuned two state-of-the-art large pre-trained language models, CodeBERT and CoTexT, for the task of vulnerability detection in programming language code, and evaluated the ability of these models to render interpretation of their output. For this, we curated a benchmark dataset composed of a collection of vulnerable code and their respective ground-truth masks, locating exactly where the vulnerability is present. We generated the heatmaps with the importance of each input token to the final prediction using the two best performing interpretability methods for text classification in the Natural Language Processing literature: Saliency and InputXGradient. We found that both methods give similar precision results, but InputXGradient heatmaps are considerably easier to interpret. Comparing the language models, CodeBERT presents a slightly inferior classification accuracy compared with CoTexT, but contrastively, the former can give considerably better interpretation results for its decision. Additionally, we highlight the effect the tokenizers have on the models' ability to generate interpretation of their predictions, showing that the tokenizer characteristics can strongly affect the model's abilities to learn the syntactic and semantic structure of the language.

Unavailability of the iliac-caval system due to thrombosis or aberrant anatomy may preclude kidney transplantation (KT) in small infants, exposing them to the complications of long-term dialysis. A tailored approach may enable KT also in these difficult patients.We report the cases of 2 pediatric patients with a history of long-term hemodialysis, a previously failed KT, pending exhaustion of vascular accesses for dialysis, and unsuitability of the iliac-caval axis as a site for KT. Both patients were successfully managed by using splenic vessels as a source of arterial inflow or venous drainage during KT. Notably, one patient also had a previous liver transplant.Both kidney grafts showed primary function. Posttransplant courses were uneventful, and no rejection episode was observed. At 64- and 10-mo follow-ups, both children had optimal renal function and excellent quality of life.When the iliac-caval system is unavailable, kidney graft implantation on splenic vessels represents a safe and effective option for pediatric KT.

A cervicalgia se caracteriza como dor na região cervical, e acomete de 48% a 78% dos estudantes de graduação. A presença de dor na região cervical pode estar relacionada ao desenvolvimento de pontos gatilhos, que são definidos como nódulos rígidos e dolorosos presentes em uma faixa tensa do músculo esquelético. Estes nódulos podem interferir nas atividades de vida diárias quando não realizado o tratamento adequado. Desta forma, realizar a técnica de acupressão em pontos gatilhos cervicais de estudantes universitários ajuda a reduzir o quadro álgico? Com base nestas informações, o objetivo deste estudo foi analisar se a aplicação da acupressão reduz a dor cervical de jovens universitários quando aplicada em pontos gatilhos. Foi realizado um estudo clínico de pré e pós-intervenção, com 10 graduandos de fisioterapia do 4° ano, idade entre 20 e 30 anos, que apresentaram quadro álgico em pontos gatilhos na região cervical e concordaram em participar da intervenção através da técnica de acupressão. De acordo com os dados obtidos no pré e pós avaliação foi possível observar melhora significativa do quadro álgico em pontos gatilhos na região cervical de graduandos de fisioterapia. Pode-se concluir que a acupressão mostrou-se eficaz para a redução da dor em pontos gatilhos em região cervical de universitários.

Internet has become one of the important needs in everyday life. Internet access is very easy with various types of devices, one of which is mobile. This opens up opportunities for business people to serve customers in carrying out mobile-based transactions or what is called m-commerce. M-Commerce provides new innovations in the business world with the convenience it offers, making it used for new business breakthroughs, one of which is delivery service as a service that pampers buyers so they don't have to wait for goods or services ordered for days. This M-Commerce application for purchasing products or ordering services is built using the angularJS method based on the Model View Controller (MVC). This makes the software development cycle more streamlined and easy. This application provides easy service to m-commerce users because of the addition of delivery service, it is also expected to increase the purchase of goods or services on a regular basis. The conveniences obtained from this application are in the form of ordering goods which will then be delivered by the courier, tracking the courier's position while in the process of ordering, selecting products and services that are easier for users to see and viewing transactions and user history, on a mobile basis.

Malathion is an insecticide that is used to control arboviruses and agricultural pests. Adolescents that are exposed to this insecticide are the most vulnerable as they are in the critical period of postnatal sexual development. This study aimed to evaluate whether malathion damage can affect sperm function and its respective mechanisms when adolescents are exposed during postnatal sexual development. Twenty-four male Wistar rats (PND 25) were divided into three experimental groups and treated daily for 40 d: control group (saline 0.9%), 10 mg/kg (M10 group), or 50 mg/kg (M50 group) of malathion. At PND 65, the rats were anesthetized and euthanized. Testicles were collected for the evaluation of gene expression. Sperm cells from the epididymis were used for evaluation of the oxidative profile or spermatic function. Data showed that a lower dose of malathion downregulated the gene expression of androgen receptors and testosterone converter enzyme 17-β-HSD in the testis. The acrosomal integrity of sperm cells was compromised in the M50 group, but not the M10 group. The mitochondrial activity was not impaired by exposure. Finally, although no alterations in malondialdehyde and glutathione levels were observed, malathion, at both doses, increased antioxidant enzyme catalase activity and, at a higher dose, superoxide dismutase activity. The present study showed that low doses of malathion considered to be inoffensive are capable of impairing sperm quality and function through the downregulation of testicular genic expression of AR enzyme 17-β-HSD and can damage the spermatic antioxidant profile during critical periods of development.

Background: Large randomized trials have demonstrated that lung cancer (LC) screening with low-dose computed tomography (LDCT) reduces LC mortality in heavy smokers. We previously showed in the MILD screening trial that the combination of a prespecified circulating microRNA (miRNA) signature classifier (MSC) and LDCT improves the accuracy of LDCT alone. The primary aim of the prospective BioMILD study was to assess the additional value of the blood MSC assay at the time of baseline LDCT with the goal of personalizing LC screening intervals. Patients and methods: The study enrolled 4119 volunteers from January 2013 to March 2016, with a median follow-up of 5.3 years. Baseline LDCT and miRNAs stratified participants into four groups: CT−/MSC− (n = 2664; 64.7%); CT−/MSC+ (n = 800; 19.4%); CT+/MSC− (n = 446; 10.8%); and CT+/MSC+ (n = 209; 5.1%). As per the protocol, those in the CT−/MSC− and CT−/MSC+ groups were allocated to LDCT repeat at 3-year and 1-year intervals; CT+ participants were allocated for 1-year or earlier intervals on the basis of LDCT features independent of MSC results. Results: CT+ participants had a 15.8-fold higher 4-year LC incidence than CT− participants (95% confidence interval 10.34-24.05), and MSC+ participants had a 2.0-fold higher 4-year LC incidence than MSC− participants (95% confidence interval 1.40-2.90); there was no evidence that the MSC effect differed between CT+ and CT− participants. LC incidence at 4 years was 0.8% in CT−/MSC−, 1.1% in CT−/MSC+, 10.8% in CT+/MSC−, and 20.1% in CT+/MSC+ participants. LC mortality rates at 5 years in the four risk groups were 0.5 in CT−/MSC−, 1.5 in CT−/MSC+, 4.2 in CT+/MSC−, and 10.1 in CT+/MSC+. Conclusion: The combined use of LDCT and blood miRNAs at baseline predicts individual LC incidence and mortality, with a major effect of MSC for LDCT-positive individuals. These findings may have important implications in personalizing screening intervals.

Monitoring is an activity in controlling activities that are mutually sustainable. In the worldof education, monitoring is one of the things that will be done to find out the academicdevelopment of a student. However, the monitoring that is usually carried out is informationcarried out between one and two people face-to-face or through short messages that havenot been computerized. It's the same with ELC courses that are still updating via shortmessages such as whatsapp. Therefore, we need an application that can be a mediumbetween parents and homeroom teachers. With this application, it is hoped that it can helphomerooms and teachers in conveying various information about students' academics. Andparents are facilitated with a real-time attendance system that can be seen in this applicationwhich was designed using the Task Centered System Design method. This method canprovide any recommendations that are needed by the user. Testing on this system is carriedout by direct interviews with the owner of the ELC as well as several employees who workat ELC who will use this application. Based on this test, the ELC Parents application cancarry out its orders in inputting student attendance and computerized student assessments.

Current developments In today's era, there are still many companies or businesses that still do not use technology. To be able to provide information, internet technology is needed. Accessing the internet can be done anytime and anywhere if a connection is available. All companies will compete with each other to use this technology to market and sell their products so that companies can develop and are able to continue to compete with other companies. efficient, the means is an online marketing and sales system. Based on this situation, the company decided to develop a website like e-commerce. Then a sales information system was made which is similar to an e-commerce website. To train how to market products and the buying and selling process, we can start using the e-commerce web. Making this system will be able to reduce problems that occur between buyers and sellers because it is done by use a system that is usually connected to internet. In addition, buyers can also view detailed product information and understand the product they want to buy. This system is made using the PHP programming language with the Laravel framework. The Laravel framework is a website development that is formed in the PHP language. The results of this research are in the form of designing a web-based online sales system that can also be used to find out detailed product information. respondents, the results of this questionnaire get a percentage value of 95% so it can be concluded that this online sales system can be used, and makes it easier for users.

We introduce a new Hopf algebra that operates on pairs of finite interval partitions and permutations of equal length. This algebra captures vincular patterns, which involve specifying both the permutation patterns and the consecutive occurrence of values. Our motivation stems from linear functionals that encode the number of occurrences of these patterns, and we show that they behave well with respect to the operations of this Hopf algebra.

The aim of this study is to present a personalized predictive model (PPM) with a machine learning (ML) system that is able to identify and classify patients with suspected prostate cancer (PCa) following mpMRI. We extracted all the patients who underwent fusion biopsy (FB) from March 2014 to December 2019, while patients from August 2020 to April 2021 were included as a validation set. The proposed system was based on the following four ML methods: a fuzzy inference system (FIS), the support vector machine (SVM), k-nearest neighbors (KNN), and self-organizing maps (SOMs). Then, a system based on fuzzy logic (FL) + SVM was compared with logistic regression (LR) and standard diagnostic tools. A total of 1448 patients were included in the training set, while 181 patients were included in the validation set. The area under the curve (AUC) of the proposed FIS + SVM model was comparable with the LR model but outperformed the other diagnostic tools. The FIS + SVM model demonstrated the best performance, in terms of negative predictive value (NPV), on the training set (78.5%); moreover, it outperformed the LR in terms of specificity (92.1% vs. 83%). Considering the validation set, our model outperformed the other methods in terms of NPV (60.7%), sensitivity (90.8%), and accuracy (69.1%). In conclusion, we successfully developed and validated a PPM tool using the FIS + SVM model to calculate the probability of PCa prior to a prostate FB, avoiding useless ones in 15% of the cases.

Resumo: Introdução: A pedagogia freiriana aponta a educação como uma prática social consoante a uma realidade em transformação e direcionada às demandas de seu público. Na educação médica, aplica-se tal concepção quando o ato pedagógico é um modelo de cuidado para os discentes. Nesse sentido, estratégias que promovem a expressão de sentimentos de forma livre, lúdica e criativa são práticas que promovem o cuidado e contribuem para uma formação humanista e reflexiva, como preconizado nas DCN para o curso de graduação em Medicina. O objetivo deste relato de experiência é compartilhar uma experiência bem-sucedida da utilização de memes em uma atividade de feedback do componente curricular de Saúde Coletiva de um curso de Medicina de uma universidade federal brasileira. Relato de experiência: No último encontro do semestre, os estudantes, reunidos em pequenas equipes, foram orientados a criar memes que refletissem suas principais percepções acerca do módulo, ou seja, que destacassem os momentos mais marcantes e os aprendizados compartilhados ao longo do semestre. Em seguida, a turma toda se reuniu por videochamada, e um representante de cada equipe fez o feedback apreciativo do semestre utilizando o meme criado, além de compartilhar o “momento a-há” elegido pelo grupo, ou seja, o mais marcante e transformador do módulo. Discussão: Percebe-se que a utilização de memes e outros recursos lúdicos na educação médica é importante e recomendada, pois, além de tornar o processo de aprendizagem mais ativo e efetivo, o que está de acordo com os princípios das DCN, é uma forma de cuidado em saúde com os discentes. Além disso, essa dinâmica permite uma maior interação entre os alunos e estimula o senso de pertencimento. Ademais, a realização de feedbacks na graduação de Medicina é fundamental para o processo de aprendizado e também para o aprimoramento de habilidades necessárias à prática profissional, e essa atividade se mostrou facilitada e efetiva quando feita por meio do uso de memes. Conclusão: A utilização de memes na sessão de feedback se mostrou benéfica, criativa e salutogênica, além de ser uma estratégia que preza o cuidado e a expressão de sentimentos dos alunos, e, por isso, deve ser encorajada. Abstract: Introduction: Freirean pedagogy points to education as a social practice in line with a reality in transformation and directed to the demands of its public. In medical education, this concept is applied when the pedagogical act is a model of care for students. Accordingly, strategies that promote a free expression of feelings, in a playful and creative way are practices that promote care and contribute to a humanistic and reflective training, as recommended in the National Curriculum Guidelines (DCNs) for undergraduate medical training. The objective of this experience report is to share a successful experience of the use of memes in a feedback activity of the Public Health curricular component of a Medicine course at a Brazilian federal university. Experience report: In the last meeting of the semester, the students were grouped into small teams and instructed to create memes that reflect their main perceptions about the module, that is, to highlight the most remarkable moments and the lessons learned during the semester. Subsequently, the whole class assembled by video call and a representative of each team gave the appreciative feedback of the semester using the created meme, in addition to sharing the “a-ha moment” chosen by the group, that is, the most striking and transformative moment of the module. Discussion: One can note that the use of memes and other recreational resources in medical education is important and recommended, because, in addition to making the learning process more active and effective, which is in accordance with the principles of the NCGs, it is also a form of care in health with students. Conclusion: It is concluded that the use of memes in the feedback session proved to be beneficial, creative and salutogenic, as well as a strategy that values the care and expression of students’ feelings and should be encouraged.

Il territorio del comune di Lecce comprende una fascia costiera lunga quasi 23km. Nel quadro del Piano Urbanistico Generale(PUG, [1]), il decisore politico locale vorrebbe valutare l'impatto del cambiamento climatico su questo territorio, in particolare per quanto riguarda l'innalzamento del livello del mare. A tal fine, abbiamo utilizzato un modello digitale del terreno ad alta risoluzione (1m x 1m) in combinazione con informazioni oceanografiche sul livello futuro del mare, nel quadro di un modello "bathtub". Sono stati considerati vari orizzonti temporali (2030, 2050, 2070) e gli scenari climatici RCP4.5 e RCP8.5. Il trend di innalzamento del livello medio mare (MSL) sulla costa leccese è confermato dal confronto tra i risultati di un modello climatico globale (IPCC AR5) e uno subregionale (Adriaclim) che fornisce una modellizzazione integrata del ciclo idrico locale del bacino adriatico con prognosticamente risolto il rilascio fluviale di oltre 100 bacini. Invece, per i livelli estremi (ESL) è stato possibile ricorrere alle previsioni di un modello globale (Kirezci et al, 2020, [2]), validato su dati storici, che tiene conto dell'escursione di marea, dello storm surgee del sovralzo d'onda. A causa di un previsto aumento del MSL nell'intervallo di 10-20 cm fino al 2050 e del pendio poco profondo della maggior parte delle spiagge, è prevista una trasgressione marina fino a pochi metri. E' stata considerata anche la futura modificazione deilivelli estremi (ESL). Apartire dal 2030, l'utilizzo diESL con tempo di ritorno di 1 anno indica come possibile l'inondazione episodicadi alcune strade perpendicolari alla costa durante gli eventi estremi di innalzamento del mare. Un altro risultato è la disomogeneità spaziale delle aree allagate, che interessano soprattuttola parte settentrionale del territorio, tra le storiche torri di avvistamento di Torre Rinalda e Torre Chianca, e soprattutto in corrispondenza delleantiche paludi costiere. L'entità dellatrasgressione del mare dipenderà sia dall'elevazione della costa che dalla sua pendenza. In particolare, le spiagge in cui il sistema dunale è stato danneggiato o rimosso, come quelle utilizzate per campetti sportivi, subiranno gli impatti più significativi e precoci. Il risultato finale di questa ricerca è influenzato da diverse incertezze, in particolare quelle relative alla combinazione dei dati (elevazione terrestre e livello del mare) che utilizzano diversi riferimenti verticali, e che viene qui discussa criticamente. Le future linee di costa georiferite ottenute in questo lavorosono pubblicate su [3]. Citare come:Mannarini, Salinas, Verri, Santos Da Costa, Barzaghi, “Futura linea di costa leccese - presentazione” (2023), DOI: 10.5281/zenodo.8009674 Riferimenti [1] https://www.puglecce.it/ [2] Kirezci et al, 2020: https://www.nature.com/articles/s41598-020-67736-6 [3]doi.org/10.5281/zenodo.8009645

In recent years, the environmental effects of both active and legacy mining activity have motivated many research groups worldwide through the use of a variety of methods that have been conducted among diverse environments. In this study, we measured radionuclide concentrations at two coastline locations of the Northern Aegean Sea: Stratoni and Ierissos. We deployed KATERINA II, an in situ gamma ray spectrometer. Our results indicate that the activity concentration for 238U progenies (214Bi), 232Th progenies (208Tl and 228Ac) and 40K vary by up to (33 ± 4) Bq kg−1, (19 ± 3) Bq kg−1, and (420 ± 30) Bq kg−1, respectively. The activity concentration of the 137Cs in Stratoni and Ierissos beach sands were (8.1 ± 2.2) and (3.9 ± 1.2) Bq kg−1, respectively. Lab-based measurements were also collected prior to the in situ data collection for the determination of radionuclide and metal concentrations. The lab-based data were found to be (800 ± 40) μg g−1 and (12 ± 1) μg g−1, for As, (1200 ± 60) μg g−1 and (33.3 ± 0.3) μg g−1 for Pb, (100 ± 6) μg g−1 and (6.0± 0.3) μg g−1 for Cu and (2000 ± 60) μg g−1 and (8.0 ± 0.4) μg g−1 for Zn, respectively. We used the R language and environment for statistical computing to produce radiological maps of the subject beach sands. We used the Enrichment Factor (EF) to estimate assessment indices for the target area and compared them to internationally recommended values. The in situ maps will be discussed since the beach area of the load-out pier area of Stratoni was undergoing the first phase of active remediation. We conclude that the temporal aspect of this dataset can be of significant reference value against future comparative studies after the remediation of the Stratoni beach with potentially denser spatial and temporal data coverage.

Modeling the ocean from the open sea to the coasts requires an adequate choice of the vertical coordinate system.A category of generalized vertical coordinates, the so-called z∗ coordinates ([Stacey et al 1995; Adcroft and Campin 2004]), has been implemented into an unstructured grid, finite elements and layer integrated model which was previously z−geopotential coordinate based.Idealized and realistic test cases are shown to assess the vertical coordinate impact on key physical processes, i.e. the internal tide generation at a continental slope, a river plume shape and intensity and the salt water intrusion.To our knowledge this is the first work that uses the z∗ coordinates in an estuary and river plume with an unstructured grid model. Other studies have used the z∗ coordinates with unstructured grid models covering the global ocean ([Ringler et al 2013, Scholz et al 2019] as examples), but without assessing their performance for the small scale dynamics of estuaries and river plumes. Coastal ocean models are currently mostly based on the terrain following coordinates, used within optimised or hybrid approaches ([Zhang et al 2015; Bruciaferri et al 2018, Fofonova et al 2021, Wise et al 2022] as examples).The results show that the z∗ coordinate model produces, with respect to z−geopotential coordinates, a stronger water column stratification. An idealized twin experiment configuration of the model to simulate the production of internal tides at the continental slope confirms that the z∗ coordinate model reproduces the expected internal tide fields while the z−geopotential model fails. A set of realistic experiments in the Po river delta coastal region, demonstrates that the representation of the dynamical processes at the coastal scales benefits from a very fine resolution of a few tens of centimeters at the surface (four layers of 25 cm are considered in the upper water column), that is only allowed by the z∗ formulation.

Brucella abortus is a zoonotic pathogen whose virulence depends on its ability to survive intracellularly at the endoplasmic reticulum derived compartment. The two-component system BvrR/BvrS (BvrRS) is essential for intracellular survival due to the transcriptional control of the type IV secretion system VirB and its transcriptional regulator VjbR. It is a master regulator of several traits including membrane homeostasis by controlling gene expression of membrane components, such as Omp25. BvrR phosphorylation is related to DNA binding at target regions, thereby repressing or activating gene transcription. To understand the role of BvrR phosphorylation we generated dominant positive and negative versions of this response regulator, mimicking phosphorylated and non-phosphorylated BvrR states and, in addition to the wild-type version, these variants were introduced in a BvrR negative background. We then characterized BvrRS-controlled phenotypes and assessed the expression of proteins regulated by the system. We found two regulatory patterns exerted by BvrR. The first pattern was represented by resistance to polymyxin and expression of Omp25 (membrane conformation) which were restored to normal levels by the dominant positive and the wild-type version, but not the dominant negative BvrR. The second pattern was represented by intracellular survival and expression of VjbR and VirB (virulence) which were, again, complemented by the wild-type and the dominant positive variants of BvrR but were also significantly restored by complementation with the dominant negative BvrR. These results indicate a differential transcriptional response of the genes controlled to the phosphorylation status of BvrR and suggest that unphosphorylated BvrR binds and impacts the expression of a subset of genes. We confirmed this hypothesis by showing that the dominant negative BvrR did not interact with the omp25 promoter whereas it could interact with vjbR promoter. Furthermore, a global transcriptional analysis revealed that a subset of genes responds to the presence of the dominant negative BvrR. Thus, BvrR possesses diverse strategies to exert transcriptional control on the genes it regulates and, consequently, impacting on the phenotypes controlled by this response regulator.

Con la revolución de septiembre de 1868 se instaura un régimen político en el que las libertades civiles se ven más respetadas, en particular la libertad de prensa, por lo cual se desarrollan desde el comienzo y de forma significativa varias tipologías de actividades editoriales, desde las satíricas hasta las políticas, vinculadas a las distintas corrientes que hacen valer sus propuestas con respecto a las instituciones que deberán remplazar a las viejas. También el carlismo se muestra más bien activo en el ámbito periodístico, cuando –por primera vez en su historia– toma el camino de la lucha pacífica, si bien sin abandonar la opción de una vía armada. Tras un apartado de contextualización y de análisis general de las características de la prensa carlista en el primer periodo del Sexenio, se pasa a un caso de estudio específico, tomando en consideración dos periódicos, uno continuación del otro: El Legitimista Español y La Legitimidad, no tan estudiados como los más conocidos La Esperanza, La Regeneración o El Pensamiento Español. La elección de estos dos periódicos permite profundizar en las cuestiones políticas a las que los carlistas han de enfrentarse en la primera parte del Sexenio, ya que su director es uno de los diputados tradicionalistas más activos en las Cortes: Cruz Ochoa, quien será, tras 1872, un convencido combatiente de la guerra civil. After the September 1868 revolution, a political new regime was established in Spain. Civil liberties were then more respected, in particular the freedom of the press. Therefore, several typologies of editorial activity were developed from the beginning and, in a significant way, both satirical and political linked to different currents that asserted their proposals, regarding the institutions destined to replace the previous ones. Carlism was also rather active in the journalistic sphere, when – for the first time in its history – it took the path of peaceful struggle, though without abandoning the option of an armed route. After some contextualization and a general analysis of the characteristics of the Carlist press in the Sexenio first period, we move on to a particular case study, taking into consideration two consequtive newspapers: El Legitimista Español and La Legitimidad, not so well studied as the best known La Esperanza, La Regeneración and El Pensamiento Español. The study of both newspapers allows us to delve into the political issues the Carlists had to face at the beginning of the Sexenio, since their editor, Cruz Ochoa, was one of the most active traditionalist deputies in the Cortes. And after 1872, he would be a convinced fighter of the Civil War.

L'articolo analizza le categorie storiografiche di orientamento sessuale e identità di genere come proprie dell'età contemporanea

Pasar tradisional merupakan salah satu tempat yang memiliki kemungkinan kontaminasi dan tempat perkembangbiakan mikroba yang tinggi. Kurangnya kesadaran pedagang mengenai kesehatan daging dapat mengakibatkan daging broiler terkontaminasi mikroorganisme patogen sehingga jika tidak ditangani dengan baik akan berakibat buruk pada kesehatan manusia. Penelitian ini bertujuan untuk melihat gambaran cemaran bakteri E. coli pada daging ayam broiler yang dijual di pasar pos Duri Jakarta Barat. Penelitian ini bersifat deskriptif. Pemeriksaan cemaran bakteri dilakukan di lab TLM STIK Kesosi pada bulan April-Mei 2022. Pada pemeriksaan mikroskopik ditemukan bakteri batang Gram negatif. Sedangkan pada pemeriksaan biokimia didapatkan uji oxidase strip negatif dan TSIA +/+ gas pada semua sampel daging ayam broiler. Berdasarkan hasil identifikasi bakteri Escherichia coli pada 30 sampel ayam Broiler di pasar pos duri maka dapat disimpulkan adalah jenis spesies bakteri Escherichia coli

The Solute Carrier 15 (SLC15) family of peptide transporters, alias H+-coupled oligopeptide cotransporter family, is a group of membrane transporters known for their key role in the cellular uptake of di- and tripeptides (di/tripeptides). Of its members, SLC15A1 (PEPT1) chiefly mediates intestinal absorption of luminal di/tripeptides from overall dietary protein digestion, SLC15A2 (PEPT2) mainly allows renal tubular reuptake of di/tripeptides from ultrafiltration and brain-to-blood efflux of di/tripeptides in the choroid plexus, SLC15A3 (PHT2) and SLC15A4 (PHT1) interact with both di/tripeptides and histidine, e.g. in certain immune cells, and SLC15A5 has unknown physiological function. In addition, the SLC15 family of peptide transporters variably interacts with a very large number of peptidomimetics and peptide-like drugs. It is conceivable, based on the currently acknowledged structural and functional differences, to divide the SLC15 family of peptide transporters into two subfamilies [3].

Polyphenols are investigated intensively in nutritional and clinical settings because of their beneficial bioactive properties and prevalence in plant-based foods. Due to their complexity, analysis with untargeted approaches is favorable. These approaches typically use high-resolution mass spectrometry (HRMS) rather than low-resolution mass spectrom-etry (LRMS). The advantages of using HRMS were evaluated in this work by thoroughly testing various untargeted techniques and available online resources. By applying iterative data-dependent acquisition on real-life urine samples, 27 features were identified with spectral libraries, 88 with in silico fragmentation, and 113 features were annotated by their monoisotopic mass with PhytoHub, an online database containing >2000 polyphenols. Moreover, potentially polyphenol-related features were searched for using various non-targeted analysis techniques including MassQL for glucuronide and sulfate neutral losses, and MetaboAnalyst for statistical analysis. As HRMS typically suffers from a sensitivity loss compared to state-of-the-art LRMS used in targeted workflows, the sensitivity gap between the two instrumental approaches was quantified. Both instruments showed feasible sensitivity, with the median limits of detection being 10 - 18 ng/mL for HRMS and 4.8 - 5.8 ng/mL for LRMS in three human matrices (urine, serum, and plas-ma). The applicability of HRMS was further evaluated in real-life urine samples with 24 polyphenols detected. The results demonstrate that despite its intrinsic limitations, HRMS can readily be used for comprehensively investigating human exposure and related health effects of polyphenols and their metabolites as well as toxicological mixture effects with other xenobiotics.