89 results on '"Vendrell J"'
Search Results
2. Role of Gastrointestinal Hormones as a Predictive Factor for Long-Term Diabetes Remission: Randomized Trial Comparing Metabolic Gastric Bypass, Sleeve Gastrectomy, and Greater Curvature Plication
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Casajoana, A, Guerrero-Perez, F, de Gordejuela, AGR, Admella, V, Sorribas, M, Vidal-Alabro, A, Virgili, N, Urdiales, RL, Montserrat, M, Perez-Maraver, M, Monasterio, C, Salord, N, Pellitero, S, Fernandez-Veledo, S, Vendrell, J, Gebelli, JP, and Vilarrasa, N
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Bariatric surgery ,Type 2 diabetes mellitus ,Obesity ,Metabolic gastric bypass ,Sleeve ,Diabetes remission ,Greater curvature plication - Abstract
Purpose Long-term studies comparing the mechanisms of different bariatric techniques for T2DM remission are scarce. We aimed to compare type 2 diabetes (T2DM) remission after a gastric bypass with a 200-cm biliopancreatic limb (mRYGB), sleeve gastrectomy (SG), and greater curvature plication (GCP), and to assess if the initial secretion of gastrointestinal hormones may predict metabolic outcomes at 5 years. Material and Methods Forty-five patients with mean BMI of 39.4(1.9)kg/m(2) and T2DM with HbA(1c) of 7.7(1.9)% were randomized to mRYGB, SG, or GCP. Anthropometric and biochemical parameters, fasting concentrations of PYY, ghrelin, glucagon, and AUC of GLP-1 after SMT were determined prior to and at months 1 and 12 after surgery. At 5-year follow-up, anthropometrical and biochemical parameters were determined. Results Total weight loss percentage (TWL%) at year 1 and GLP-1 AUC at months 1 and 12 were higher in the mRYGB than in the SG and GCP. TWL% remained greater at 5 years in mRYGB group - 27.32 (7.8) vs. SG - 18.00 (10.6) and GCP - 14.83 (7.8), p = 0.001. At 5 years, complete T2DM remission was observed in 46.7% after mRYGB vs. 20.0% after SG and 6.6% after GCP, p < 0.001. In the multivariate analysis, shorter T2DM duration (OR 0.186), p = 0.008, and the GLP-1 AUC at 1 month (OR 7.229), p = 0.023, were prognostic factors for complete T2DM remission at 5-year follow-up. Conclusions Long-term T2DM remission is mostly achieved with hypoabsortive techniques such as mRYGB. Increased secretion of GLP-1 after surgery and shorter disease duration were the main predictors of T2DM remission at 5 years.
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- 2021
3. Adipose stem cells from patients with Crohn's disease show a distinctive DNA methylation pattern
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Serena, C, Millan, M, Ejarque, M, Saera-Vila, A, Maymo-Masip, E, Nunez-Roa, C, Monfort-Ferre, D, Terron-Puig, M, Bautista, M, Menacho, M, Marti, M, Espin, E, Vendrell, J, and Fernandez-Veledo, S
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Adipose tissue ,Methylome ,Epigenetics ,Gene expression ,Inflammatory bowel disease - Abstract
Background Crohn's disease (CD) is characterized by persistent inflammation and ulceration of the small or large bowel, and expansion of mesenteric adipose tissue, termed creeping fat (CF). We previously demonstrated that human adipose-derived stem cells (hASCs) from CF of patients with CD exhibit dysfunctional phenotypes, including a pro-inflammatory profile, high phagocytic capacity, and weak immunosuppressive properties. Importantly, these phenotypes persist in patients in remission and are found in all adipose depots explored including subcutaneous fat. We hypothesized that changes in hASCs are a consequence of epigenetic modifications. Methods We applied epigenome-wide profiling with a methylation array (Illumina EPIC/850k array) and gene expression analysis to explore the impact of CD on the methylation signature of hASCs isolated from the subcutaneous fat of patients with CD and healthy controls (n = 7 and 5, respectively; cohort I). Differentially methylated positions (p value cutoff < 1 x 10(-4) and ten or more DMPs per gene) and regions (inclusion threshold 0.2, p value cutoff < 1 x 10(-2) and more than 2 DMRs per gene) were identified using dmpfinder and Bumphunter (minfi), respectively. Changes in the expression of differentially methylated genes in hASCs were validated in a second cohort (n = 10/10 inactive and active CD and 10 controls; including patients from cohort I) and also in peripheral blood mononuclear cells (PBMCs) of patients with active/inactive CD and of healthy controls (cohort III; n = 30 independent subjects). Results We found a distinct DNA methylation landscape in hASCs from patients with CD, leading to changes in the expression of differentially methylated genes involved in immune response, metabolic, cell differentiation, and development processes. Notably, the expression of several of these genes in hASCs and PBMCs such as tumor necrosis factor alpha (TNFA) and PR domain zinc finger protein 16 (PRDM16) were not restored to normal (healthy) levels after disease remission. Conclusions hASCs of patients with CD exhibit a unique DNA methylation and gene expression profile, but the expression of several genes are only partially restored in patients with inactive CD, both in hASCs and PBMCs. Understanding how CD shapes the functionality of hASCs is critical for investigating the complex pathophysiology of this disease, as well as for the success of cell-based therapies.
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- 2020
4. Impaired Succinate Response to a Mixed Meal in Obesity and Type 2 Diabetes Is Normalized After Metabolic Surgery
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Astiarraga B, Martínez L, Ceperuelo-Mallafré V, Llauradó G, Terrón-Puig M, Mar Rodríguez M, Casajoana A, Pellitero S, Megía A, Vilarrasa N, Vendrell J, and Fernández-Veledo S
- Abstract
To explore the meal response of circulating succinate in patients with obesity and type 2 diabetes undergoing bariatric surgery and to examine the role of gastrointestinal glucose sensing in succinate dynamics in healthy subjects.
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- 2020
5. Synthesis and Structural/Functional Characterization of Selective M14 Metallocarboxypeptidase Inhibitors Based on Phosphinic Pseudopeptide Scaffold: Implications on the Design of Specific Optical Probes
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Covaleda, G. Gallego, P. Vendrell, J. Georgiadis, D. Lorenzo, J. Dive, V. Aviles, F.X. Reverter, D. Devel, L.
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Metallocarboxypeptidases (MCPs) of the M14 family are Zn 2+ -dependent exoproteases present in almost every tissue or fluid in mammals. These enzymes perform a large variety of physiological functions and are involved in several pathologies, such as pancreatic diseases, inflammation, fibrinolysis, and cancer. Here, we describe the synthesis and functional/structural characterization of a series of reversible tight-binding phosphinic pseudopeptide inhibitors that show high specificity and potency toward these proteases. Characterization of their inhibitory potential against a large variety of MCPs, combined with high-resolution crystal structures of three selected candidates in complex with human carboxypeptidase A (CPA)1, allowed to decipher the structural determinants governing selectivity for type-A of the M14A MCP family. Further, the phosphinic pseudopeptide framework was exploited to generate an optical probe selectively targeting human CPAs. The phosphinic pseudopeptides presented here constitute the first example of chemical probes useful to selectively report on type-A MCPs activity in complex media. © 2019 American Chemical Society.
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- 2019
6. Corrigendum to 'The BACE1 product sAPP induces ER stress and inflammation and impairs insulin signaling' [Metab Clin Exp 85 (2018) 59-75]
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Botteri G, Salvadó L, Gumà A, Hamilton DL, Meakin PJ, Montagut G, Ashford MLJ, Ceperuelo-Mallafré V, Fernández-Veledo S, Vendrell J, Calderón-Dominguez M, Serra D, Herrero L, Pizarro J, Barroso E, Palomer FX, and Vazquez M
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- 2019
7. Pre-operative circulating succinate levels as a biomarker for diabetes remission after bariatric surgery
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Ceperuelo-Mallafre, V, Llaurado, G, Keiran, N, Benaiges, E, Astiarraga, B, Martinez, L, Pellitero, S, Gonzalez-Clemente, J, Megia, A, Vilarrasa, N, Vendrell, J, and Fernandez-Veledo, S
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- 2019
8. Preoperative Circulating Succinate Levels as a Biomarker for Diabetes Remission After Bariatric Surgery
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Ceperuelo-Mallafre, V, Llaurado, G, Keiran, N, Benaiges, E, Astiarraga, B, Martinez, L, Pellitero, S, Gonzalez-Clemente, JM, Rodriguez, A, Fernandez-Real, JM, Lecube, A, Megia, A, Vilarrasa, N, Vendrell, J, and Fernandez-Veledo, S
- Abstract
OBJECTIVE To determine the potential use of baseline circulating succinate to predict type 2 diabetes remission after bariatric surgery. RESEARCH DESIGN AND METHODS Forty-five obese patients with diabetes were randomly assigned to Roux-en-Y gastric bypass (RYGB), sleeve gastrectomy (SG), or laparoscopic greater curvature plication. Anthropometric parameters were evaluated, and a complete biochemical analysis including circulating serum succinate concentrations was performed at baseline and 1 year after surgery. The results were externally validated in a second cohort including 88 obese patients with diabetes assigned to RYGB or SG based on clinical criteria. RESULTS Succinate baseline concentrations were an independent predictor of diabetes remission after bariatric surgery. Patients achieving remission after 1 year had lower levels of baseline succinate (47.8 [37.6-64.6] mu mol/L vs. 64.1 [52.5-82.9] mu mol/L; P = 0.018). Moreover, succinate concentrations were significantly decreased 1 year after surgery (58.9 [46.4-82.4] mu mol/L vs. 46.0 [35.8-65.3] mu mol/L, P = 0.005). In multivariate analysis, the best logistic regression model showed that baseline succinate (odds ratio [OR] 11.3, P = 0.031) and the type of surgery (OR 26.4, P = 0.010) were independently associated with remission. The C-statistic for this model was 0.899 (95% CI 0.809-0.989) in the derivation cohort, which significantly improved the prediction of remission compared with current available scores, and 0.729 (95% CI 0.612-0.846) in the validation cohort. Interestingly, patients had a different response to the type of surgery according to baseline succinate, with significant differences in remission rates. CONCLUSIONS Circulating succinate is reduced after bariatric surgery. Baseline succinate levels have predictive value for diabetes remission independently of previously described presurgical factors and improve upon the current available scores to predict remission.
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- 2019
9. Extended-spectrum beta-lactamase-producing Escherichia coli in common vampire bats Desmodus rotundus and livestock in Peru
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Benavides, J. A., Shiva, C., Virhuez, M., Tello, C., Appelgren, A., Vendrell, J., Solassol, J., Godreuil, S., Streicker, D. G., University of Glasgow, Universidad Peruana Cayetano Heredia (UPCH), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Institut de Recherche en Cancérologie de Montpellier (IRCM - U1194 Inserm - UM), CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Pathogénèse et contrôle des infections chroniques (PCCI), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), MRC - University of Glasgow Centre for Virus Research, and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier )
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antibiotic resistance ,bat ,multilocus sequence typing ,disease carrier ,Chiroptera ,Drug Resistance, Multiple, Bacterial ,Zoonoses ,Peru ,plasmid typing ,animal ,genetics ,Escherichia coli infection ,ComputingMilieux_MISCELLANEOUS ,Escherichia coli Infections ,sheep disease ,bovine ,drug effect ,transmission ,unclassified drug ,wild animal ,Anti-Bacterial Agents ,antiinfective agent ,priority journal ,Animals, Domestic ,ESBL-producing Escherichia coli ,bacterial gene ,ESBL‐producing Escherichia coli ,Plasmids ,sheep ,Livestock ,beta lactamase CTX M ,enzymology ,Short Communication ,Short Communications ,Cattle Diseases ,Sheep Diseases ,Animals, Wild ,Desmodus rotundus ,Article ,beta-Lactamases ,beta lactamase ,multidrug resistance ,plasmid ,Escherichia coli ,Animals ,Humans ,human ,Disease Reservoirs ,extended spectrum beta lactamase producing Escherichia coli ,cattle disease ,nonhuman ,Sheep ,isolation and purification ,microbiology ,zoonosis ,beta lactamase CTX M15 ,veterinary medicine ,domestic animal ,gene expression ,Cattle ,biosynthesis ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
International audience; Antibiotic resistance mediated by bacterial production of extended-spectrum beta-lactamase (ESBL) is a global threat to public health. ESBL resistance is most commonly hospital-acquired; however, infections acquired outside of hospital settings have raised concerns over the role of livestock and wildlife in the zoonotic spread of ESBL-producing bacteria. Only limited data are available on the circulation of ESBL-producing bacteria in animals. Here, we report ESBL-producing Escherichia coli in wild common vampire bats Desmodus rotundus and livestock near Lima, Peru. Molecular analyses revealed that most of this resistance resulted from the expression of blaCTX-M-15 genes carried by plasmids, which are disseminating worldwide in hospital settings and have also been observed in healthy children of Peru. Multilocus sequence typing showed a diverse pool of E. coli strains carrying this resistance that were not always host species-specific, suggesting sharing of strains between species or infection from a common source. This study shows widespread ESBL resistance in wild and domestic animals, supporting animal communities as a potential source of resistance. Future work is needed to elucidate the role of bats in the dissemination of antibiotic-resistant strains of public health importance and to understand the origin of the observed resistance.
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- 2018
10. Extended-spectrum beta-lactamases-producing Escherichia coli in common vampire bats Desmodus rotundus and livestock in Peru
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Benavides, J.A., Shiva, C., Virhuez, M., Tello, C., Appelgren, A., Vendrell, J., Solassol, J., Godreuil, S., and Streicker, D.G.
- Abstract
Antibiotic resistance mediated by bacterial production of extended‐spectrum beta‐lactamase (ESBL) is a global threat to public health. ESBL resistance is most commonly hospital‐acquired; however, infections acquired outside of hospital settings have raised concerns over the role of livestock and wildlife in the zoonotic spread of ESBL‐producing bacteria. Only limited data are available on the circulation of ESBL‐producing bacteria in animals. Here, we report ESBL‐producing Escherichia coli in wild common vampire bats Desmodus rotundus and livestock near Lima, Peru. Molecular analyses revealed that most of this resistance resulted from the expression of blaCTX‐M‐15 genes carried by plasmids, which are disseminating worldwide in hospital settings and have also been observed in healthy children of Peru. Multilocus sequence typing showed a diverse pool of E. coli strains carrying this resistance that were not always host species‐specific, suggesting sharing of strains between species or infection from a common source. This study shows widespread ESBL resistance in wild and domestic animals, supporting animal communities as a potential source of resistance. Future work is needed to elucidate the role of bats in the dissemination of antibiotic‐resistant strains of public health importance and to understand the origin of the observed resistance.
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- 2018
11. The BACE1 product sAPPß induces ER stress and inflammation and impairs insulin signaling
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Botteri G, Salvadó L, Gumà A, Lee Hamilton D, Meakin PJ, Montagut G, Ashford MLJ, Ceperuelo-Mallafré V, Fernández-Veledo S, Vendrell J, Calderón-Dominguez M, Serra D, Herrero L, Pizarro J, Barroso E, Palomer FX, and Vazquez M
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CREB ,PGC-1a ,mental disorders ,insulin resistance ,sAPPß ,NF-?B ,BACE1 ,palmitate - Abstract
OBJECTIVE: ß-secretase/ß-site amyloid precursor protein (APP)-cleaving enzyme 1 (BACE1) is a key enzyme involved in Alzheimer's disease that has recently been implicated in insulin-independent glucose uptake in myotubes. However, it is presently unknown whether BACE1 and the product of its activity, soluble APPß (sAPPß), contribute to lipid-induced inflammation and insulin resistance in skeletal muscle cells. MATERIALS/METHODS: Studies were conducted in mouse C2C12 myotubes, skeletal muscle from Bace1(-/-)mice and mice treated with sAPPß and adipose tissue and plasma from obese and type 2 diabetic patients. RESULTS: We show that BACE1 inhibition or knockdown attenuates palmitate-induced endoplasmic reticulum (ER) stress, inflammation, and insulin resistance and prevents the reduction in Peroxisome Proliferator-Activated Receptor ? Co-activator 1a (PGC-1a) and fatty acid oxidation caused by palmitate in myotubes. The effects of palmitate on ER stress, inflammation, insulin resistance, PGC-1a down-regulation, and fatty acid oxidation were mimicked by soluble APPß in vitro. BACE1 expression was increased in subcutaneous adipose tissue of obese and type 2 diabetic patients and this was accompanied by a decrease in PGC-1a mRNA levels and by an increase in sAPPß plasma levels of obese type 2 diabetic patients compared to obese non-diabetic subjects. Acute sAPPß administration to mice reduced PGC-1a levels and increased inflammation in skeletal muscle and decreased insulin sensitivity. CONCLUSIONS: Collectively, these findings indicate that the BACE1 product sAPPß is a key determinant in ER stress, inflammation and insulin resistance in skeletal muscle and gluconeogenesis in liver.
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- 2018
12. Elevated circulating levels of succinate in human obesity are linked to specific gut microbiota
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Serena C, Ceperuelo-Mallafré V, Keiran N, Queipo-Ortuño MI, Bernal R, Gomez-Huelgas R, Urpi-Sarda M, Sabater M, Pérez-Brocal V, Andrés-Lacueva C, Moya A, Tinahones FJ, Fernández-Real JM, Vendrell J, and Fernández-Veledo S
- Abstract
Gut microbiota-related metabolites are potential clinical biomarkers for cardiovascular disease (CVD). Circulating succinate, a metabolite produced by both microbiota and the host, is increased in hypertension, ischemic heart disease, and type 2 diabetes. We aimed to analyze systemic levels of succinate in obesity, a major risk factor for CVD, and its relationship with gut microbiome. We explored the association of circulating succinate with specific metagenomic signatures in cross-sectional and prospective cohorts of Caucasian Spanish subjects. Obesity was associated with elevated levels of circulating succinate concomitant with impaired glucose metabolism. This increase was associated with specific changes in gut microbiota related to succinate metabolism: a higher relative abundance of succinate-producing Prevotellaceae (P) and Veillonellaceae (V), and a lower relative abundance of succinate-consuming Odoribacteraceae (O) and Clostridaceae (C) in obese individuals, with the (P + V/O + C) ratio being a main determinant of plasma succinate. Weight loss intervention decreased (P + V/O + C) ratio coincident with the reduction in circulating succinate. In the spontaneous evolution after good dietary advice, alterations in circulating succinate levels were linked to specific metagenomic signatures associated with carbohydrate metabolism and energy production with independence of body weight change. Our data support the importance of microbe-microbe interactions for the metabolite signature of gut microbiome and uncover succinate as a potential microbiota-derived metabolite related to CVD risk.
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- 2018
13. GNIP1 E3 ubiquitin ligase is a novel player in regulating glycogen metabolism in skeletal muscle
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Montori M, Pedreira-Casahuga R, Boyer-Díaz Z, Lassot I, García-Martínez C, Orozco A, Cebrià-Romero J, Osorio-Conles O, Chacón MR, Vendrell J, Vazquez M, Desagher S, Jimenez-Chillaron JC, and Gómez-Foix AM
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Glycogen metabolism ,TRIM7 isoform 1 [*GNIP1] ,Skeletal muscle ,TRIM7 isoform 4 [*TRIM7] - Abstract
BACKGROUND: Glycogenin-interacting protein 1 (GNIP1) is a tripartite motif (TRIM) protein with E3 ubiquitin ligase activity that interacts with glycogenin. These data suggest that GNIP1 could play a major role in the control of glycogen metabolism. However, direct evidence based on functional analysis remains to be obtained. OBJECTIVES: The aim of this study was 1) to define the expression pattern of glycogenin-interacting protein/Tripartite motif containing protein 7 (GNIP/TRIM7) isoforms in humans, 2) to test their ubiquitin E3 ligase activity, and 3) to analyze the functional effects of GNIP1 on muscle glucose/glycogen metabolism both in human cultured cells and in vivo in mice. RESULTS: We show that GNIP1 was the most abundant GNIP/TRIM7 isoform in human skeletal muscle, whereas in cardiac muscle only TRIM7 was expressed. GNIP1 and TRIM7 had autoubiquitination activity in vitro and were localized in the Golgi apparatus and cytosol respectively in LHCN-M2 myoblasts. GNIP1 overexpression increased glucose uptake in LHCN-M2 myotubes. Overexpression of GNIP1 in mouse muscle in vivo increased glycogen content, glycogen synthase (GS) activity and phospho-GSK-3a/ß (Ser21/9) and phospho-Akt (Ser473) content, whereas decreased GS phosphorylation in Ser640. These modifications led to decreased blood glucose levels, lactate levels and body weight, without changing whole-body insulin or glucose tolerance in mouse. CONCLUSION: GNIP1 is an ubiquitin ligase with a markedly glycogenic effect in skeletal muscle.
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- 2018
14. Type 1 diabetes: Developing the first riskestimation model for predicting silent myocardial ischemia. The potential role of insulin resistance
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Vendrell, J., Llauradó, G., Cano, A., Hernández, C., González-Sastre, M., Rodríguez, A.-A., Puntí, J., Berlanga, E., Albert, L., Simó, R., Clemente, J.-M.G., Grup de Recerca Biomèdica HJ23, Medicina i Cirurgia, and Universitat Rovira i Virgili
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Ciències de la salut ,Insulinoresistència ,Diabetis ,Malalties coronàries -- Prevenció ,Està en blanc ,Health sciences ,Ciencias de la salud ,1932-6203 - Abstract
Objectives: The aim of the study was to develop a novel risk estimation model for predicting silent myocardial ischemia (SMI) in patients with type 1 diabetes (T1DM) and no clinical cardiovascular disease, evaluating the potential role of insulin resistance in such a model. Additionally, the accuracy of this model was compared with currently available models for predicting clinical coronary artery disease (CAD) in general and diabetic populations. Research, design and methods: Patients with T1DM (35-65years, >10-year duration) and no clinical cardiovascular disease were consecutively evaluated for: 1) clinical and anthropometric data (including classical cardiovascular risk factors), 2) insulin sensitivity (estimate of glucose disposal rate (eGDR)), and 3) SMI diagnosed by stress myocardial perfusion gated SPECTs. Results: Eighty-four T1DM patients were evaluated [50.1±9.3 years, 50% men, 36.9% active smokers, T1DM duration: 19.0(15.9-27.5) years and eGDR 7.8(5.5-9.4)mg·kg-1·min-1]. Of these, ten were diagnosed with SMI (11.9%). Multivariate logistic regression models showed that only eGDR (OR = -0.593, p = 0.005) and active smoking (OR = 7.964, p = 0.018) were independently associated with SMI. The AUC of the ROC curve of this risk estimation model for predicting SMI was 0.833 (95%CI:0.692-0.974), higher than those obtained with the use of currently available models for predicting clinical CAD (Framingham Risk Equation: 0.833 vs. 0.688, p = 0.122; UKPDS Risk Engine (0.833 vs. 0.559; p = 0.001) and EDC equation: 0.833 vs. 0.558, p = 0.027). Conclusion: This study provides the first ever reported risk-estimation model for predicting SMI in T1DM. The model only includes insulin resistance and active smoking as main predictors of SMI.
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- 2017
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15. Reference Values for TSH may be Inadequate to Define Hypothyroidism in Persons with Morbid Obesity: Di@bet.es Study
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Valdés S, Maldonado-Araque C, Lago-Sampedro A, Lillo-Muñoz JA, Garcia-Fuentes E, Perez-Valero V, Gutiérrez-Repiso C, Garcia-Escobar E, Goday A, Urrutia I, Peláez L, Calle-Pascual A, Bordiú E, Castaño L, Castell C, Delgado E, Menéndez E, Franch-Nadal J, Gaztambide S, Girbés J, Ortega E, Vendrell J, Chacón MR, Javier Chaves F, Soriguer F, and Rojo-Martínez G
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Adult ,Aged, 80 and over ,Male ,Observer Variation ,Adolescent ,Thyrotropin ,Middle Aged ,Overweight ,Thyroid Function Tests ,Obesity, Morbid ,Thyroxine ,Young Adult ,Cross-Sectional Studies ,Hypothyroidism ,Thinness ,Reference Values ,Spain ,Prevalence ,Humans ,Triiodothyronine ,Female ,Aged ,Autoantibodies - Abstract
Objective: To analyze the reference range of thyroid-stimulating hormone (TSH) in different BMI categories and its impact on the classification of hypothyroidism. Methods: The study included 3,928 individuals free of thyroid disease (without previous thyroid disease, no interfering medications, TSH < 10 mu UI/mL and thyroid peroxidase antibodies [TPO Abs] < 50 IU/mL) who participated in a national, cross-sectional, population-based study and were representative of the adult population of Spain. Data gathered included clinical and demographic characteristics, physical examination, and blood and urine sampling. TSH, free thyroxine, free triiodothyronine, and TPO Ab were analyzed by electrochemiluminescence (E170, Roche Diagnostics, Basel, Switzerland). Results: The reference range (p2.5-97.5) for TSH was estimated as 0.6 to 4.8 mu UI/mL in the underweight category (BMI= 40). By using the reference criteria for the normal-weight population, the prevalence of high TSH levels increased threefold in the morbid obesity category (P < 0.01). Conclusions: Persons with morbid obesity might be inappropriately classified if the standard ranges of normality of TSH for the normal-weight population are applied to them.
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- 2017
16. Multi-center evaluation of the fully automated PCR-based Idylla™ KRAS mutation assay for rapid KRAS mutation status determination on formalin-fixed paraffin-embedded tissue of human colorectal cancer
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Solassol, J, Vendrell, J, Märkl, B, Haas, C, Bellosillo, B, Montagut, C, Smith, M, O'Sullivan, B, D'Haene, N, Le Mercier, M, Grauslund, M, Melchior, L, Burt, E, Cotter, F, Stieber, D, Schmitt, F, Motta, V, Lauricella, C, Colling, R, Soilleux, E, Fassan, M, Mescoli, C, Collin, C, Pagès, J, Sillekens, P, Institut de Recherche en Cancérologie de Montpellier (IRCM - U1194 Inserm - UM), CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), CRLCC Val d'Aurelle - Paul Lamarque, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), Soilleux, Elizabeth [0000-0002-4032-7249], and Apollo - University of Cambridge Repository
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0301 basic medicine ,Neuroblastoma RAS viral oncogene homolog ,Oncology ,Genetics and Molecular Biology (all) ,Colorectal cancer ,Gene Identification and Analysis ,Psychologie appliquée ,lcsh:Medicine ,Artificial Gene Amplification and Extension ,Medicine (all) ,Biochemistry, Genetics and Molecular Biology (all) ,Agricultural and Biological Sciences (all) ,medicine.disease_cause ,Bioinformatics ,Polymerase Chain Reaction ,Biochemistry ,0302 clinical medicine ,Sequencing techniques ,Medicine and Health Sciences ,Epidermal growth factor receptor ,DNA sequencing ,lcsh:Science ,Cancer ,Multidisciplinary ,biology ,Sciences bio-médicales et agricoles ,3. Good health ,Colo-Rectal Cancer ,Clinical Medicine and Science ,030220 oncology & carcinogenesis ,Mutation (genetic algorithm) ,KRAS ,Anatomy ,Biologie ,Còlon -- Càncer -- Aspectes genètics ,Research Article ,medicine.medical_specialty ,Colon ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,Research and Analysis Methods ,03 medical and health sciences ,Clinical Research ,Internal medicine ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,medicine ,Genetics ,Point Mutation ,1112 Oncology and Carcinogenesis ,Genetic Testing ,ddc:610 ,Molecular Biology Techniques ,Genotyping ,Mutation Detection ,Molecular Biology ,Colorectal Cancer ,Point mutation ,lcsh:R ,Dideoxy DNA sequencing ,Biology and Life Sciences ,Cancers and Neoplasms ,[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology ,Molecular diagnostics ,medicine.disease ,digestive system diseases ,4.1 Discovery and preclinical testing of markers and technologies ,Gastrointestinal Tract ,030104 developmental biology ,Mutation ,biology.protein ,lcsh:Q ,Amplification-Refractory Mutation System Analysis ,Digestive Diseases ,Digestive System - Abstract
Since the advent of monoclonal antibodies against epidermal growth factor receptor (EGFR) in colorectal cancer therapy, the determination of RAS mutational status is needed for therapeutic decision-making. Most prevalent in colorectal cancer are KRAS exon 2 mutations (40% prevalence); lower prevalence is observed for KRAS exon 3 and 4 mutations (6%) and NRAS exon 2, 3, and 4 mutations (5%). The Idylla™ KRAS Mutation Test on the molecular diagnostics Idylla™ platform is a simple (, SCOPUS: ar.j, info:eu-repo/semantics/published
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- 2016
17. PPARß/d ameliorates fructose-induced insulin resistance in adipocytes by preventing Nrf2 activation
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Barroso E, Rodríguez-Rodríguez R, Chacón MR, Maymó-Masip E, Ferrer L, Salvadó L, Salmerón E, Wabistch M, Palomer FX, Vendrell J, Wahli W, and Vazquez M
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Adipocyte, CD36, Fructose, JNK, Oxidized LDL, PPARß/d - Abstract
We studied whether PPARß/d deficiency modifies the effects of high fructose intake (30% fructose in drinking water) on glucose tolerance and adipose tissue dysfunction, focusing on the CD36-dependent pathway that enhances adipose tissue inflammation and impairs insulin signaling. Fructose intake for 8 weeks significantly increased body and liver weight, and hepatic triglyceride accumulation in PPARß/d-deficient mice but not in wild-type mice. Feeding PPARß/d-deficient mice with fructose exacerbated glucose intolerance and led to macrophage infiltration, inflammation, enhanced mRNA and protein levels of CD36, and activation of the JNK pathway in white adipose tissue compared to those of water-fed PPARß/d-deficient mice. Cultured adipocytes exposed to fructose also exhibited increased CD36 protein levels and this increase was prevented by the PPARß/d activator GW501516. Interestingly, the levels of the nuclear factor E2-related factor 2 (Nrf2), a transcription factor reported to up-regulate Cd36 expression and to impair insulin signaling, were increased in fructose-exposed adipocytes whereas co-incubation with GW501516 abolished this increase. In agreement with Nrf2 playing a role in the fructose-induced CD36 protein level increases, the Nrf2 inhibitor trigonelline prevented the increase and the reduction in insulin-stimulated AKT phosphorylation caused by fructose in adipocytes. Protein levels of the well-known Nrf2 target gene
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- 2015
18. The addition of fermented milk with plant sterols improves the adherence to lifestyle changes in hypercholesterolemic patients. The RECIPE study
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Masana L., Lagares M., Pinto X., Reinares L., Zuniga M., Descamps O., Bosi E., Allaert F. A., Chapman J., Bruckert E., Hernandez T., Martin C., Gomes H., Perez P. J., Gil E. M., Moro A., Jorda M. D., Lopez J. L., Alba M., Bilbao I., Alustiza M. R., Aliaga M., Munoz J. G., Terol A. M., Gomez P., Marcos M. A., Gonzalez M., Rodriguez M. J., Lopez M. V., Romero M. A., Puyo N., Egido J. F., Monreal I., Ramirez A., Madariaga M. A., De Miguel M. C., Lorda A., Munoz G., Moreno I. M., Martinez A., Sanchez A., Espino A., Gonzalez. F. J., Pracht C., Ocana C., Pena M. A., Domingo C., Gilaberte M. T., Garcia C., Diez J. A., Fornes F. V., Gutierrez M. D., Macarra E., Bugella J. I., Del. J. M., Bravo A., Becerra M. J., Carbonell C., De Miguel M., Perez A., Martin A., Romero J., Gonzalez P., Iglesias A., Lopez B., Villalonga G., Carvajal P., Marin C., Cardona F., Gonzalez A., Fernandez G., Gomez D., Sanchez C. L., Viveros D., De Lucas M. G., Marin J. M., Osorno J., Pumares C., Gimenez R., Latorre J., Barreiro M. C., Villanueva J. R., Lopez J. A., Balaguer I., Baron J., Espuga M., Firas R., Trias F., Moran A., Velasco V., Vazquez F., Hernando A., Manzanares A., Abad J. L., Peraferrer M., Obarrio A., Alonso M. L., Gomez L., Roca J., Ayus B. E., Urban F., Gonzalez E., Masegosa C., Barrilero E., Jimenez M. C., Carratala M. M., Petitbo D., Ferrandiz J., Mialdea M. J., Montoro J., Muncharaz M. E., Shehchar A., De Paula L., Cano A., Sanchez L., Perez J., Alarcon C., Martinez J. F., Garrido T., Martinez F., Gomariz I., Caparros M., Kseibi T., Palomo L., Jimenez T., Luque I., Villar P., Gonzalez J. J., Aparicio J., Jimenez O., Munoz J. J., Baez F., Marcos M. P., Gonzalez T., Estevez J. A., Garcia J., Ajuria-Gogeasko S., Cabrera M., Manzano J. M., Segura G., Abat X., Yanovsky N., Borrachero J. M., Fortuny M., Martinez M. D., Gutierrez A. R., Najem N., Romero M. C., Martin S., Valverde R. J., Molina C., Martinez B., Izquierdo L., Vazquez I., Martinez M. T., Mateos A., Quiles F., Aranda F. J., Iborra R., Valero A. B., Fornes M. T., Quinza V., Colado F., Arribas B., Martinez J. E. L., Garces M. P., Valero J. M., Frigola J. L., Rubio J. M., Gongora A., Triana J., Gomez C., Garcia M. L., Diez C., Pombo G., Gutierrez M. C., Nazara C. A., De Oca M. A., Maquieira N., Pilo A., Buenadicha J. L., Duran F., Pena J. E., Navarro S., Serrano F. J., Sanchez S., Mesa S., Salazar J. A., De La Escosura A., Perez F., Solera J., Tarraga P., Ramos L., Gutierrez J. A., Caldelas S., Albinana F., Lopez J. F., Florian J., Rocha E., Rodriguez J., Mellado M. L., Calvillo M. R., Cruz T., Capitan M., Diaz J., Tome J., Prieto A., Tome V., Caro M., Montero A., Ontoria M., Garcia D. L., Gil F., De Zarate V., Casaponsa J., Matamala J., Salvande A., Trueba M. A., Cidra J. L., Garcia A., Toro A., Berna J. R., Zornoza B., Prieto M. T., Garcia L., Lopez C., Garcia P., Echegaray M. C., Navarro B., Moro M. L., Herrero A., Tamargo C., Perez S. J., Antero E., Penacho M. C., Blanco Y., Lucena J., Sisto C. A., Salido E., Dolz F., Granja J. M., Saavedra F., Sanchez-Oro I., Mas-Magro F., Artundo T., Fernandez A., Vaquer J. V., Aso K., Palacios A., Morante J. L., Seller T., Vendrell J. M., Masana, L., Lagares, M., Pinto, X., Reinares, L., Zuniga, M., Descamps, O., Bosi, E., Allaert, F. A., Chapman, J., Bruckert, E., Hernandez, T., Martin, C., Gomes, H., Perez, P. J., Gil, E. M., Moro, A., Jorda, M. D., Lopez, J. L., Alba, M., Bilbao, I., Alustiza, M. R., Aliaga, M., Munoz, J. G., Terol, A. M., Gomez, P., Marcos, M. A., Gonzalez, M., Rodriguez, M. J., Lopez, M. V., Romero, M. A., Puyo, N., Egido, J. F., Monreal, I., Ramirez, A., Madariaga, M. A., De Miguel, M. C., Lorda, A., Munoz, G., Moreno, I. M., Martinez, A., Sanchez, A., Espino, A., Gonzale, z. F. J., Pracht, C., Ocana, C., Pena, M. A., Domingo, C., Gilaberte, M. T., Garcia, C., Diez, J. A., Fornes, F. V., Gutierrez, M. D., Macarra, E., Bugella, J. I., De, l. J. M., Bravo, A., Becerra, M. J., Carbonell, C., De Miguel, M., Perez, A., Martin, A., Romero, J., Gonzalez, P., Iglesias, A., Lopez, B., Villalonga, G., Carvajal, P., Marin, C., Cardona, F., Gonzalez, A., Fernandez, G., Gomez, D., Sanchez, C. L., Viveros, D., De Lucas, M. G., Marin, J. M., Osorno, J., Pumares, C., Gimenez, R., Latorre, J., Barreiro, M. C., Villanueva, J. R., Lopez, J. A., Balaguer, I., Baron, J., Espuga, M., Firas, R., Trias, F., Moran, A., Velasco, V., Vazquez, F., Hernando, A., Manzanares, A., Abad, J. L., Peraferrer, M., Obarrio, A., Alonso, M. L., Gomez, L., Roca, J., Ayus, B. E., Urban, F., Gonzalez, E., Masegosa, C., Barrilero, E., Jimenez, M. C., Carratala, M. M., Petitbo, D., Ferrandiz, J., Mialdea, M. J., Montoro, J., Muncharaz, M. E., Shehchar, A., De Paula, L., Cano, A., Sanchez, L., Perez, J., Alarcon, C., Martinez, J. F., Garrido, T., Martinez, F., Gomariz, I., Caparros, M., Kseibi, T., Palomo, L., Jimenez, T., Luque, I., Villar, P., Gonzalez, J. J., Aparicio, J., Jimenez, O., Munoz, J. J., Baez, F., Marcos, M. P., Gonzalez, T., Estevez, J. A., Garcia, J., Ajuria-Gogeasko, S., Cabrera, M., Manzano, J. M., Segura, G., Abat, X., Yanovsky, N., Borrachero, J. M., Fortuny, M., Martinez, M. D., Gutierrez, A. R., Najem, N., Romero, M. C., Martin, S., Valverde, R. J., Molina, C., Martinez, B., Izquierdo, L., Vazquez, I., Martinez, M. T., Mateos, A., Quiles, F., Aranda, F. J., Iborra, R., Valero, A. B., Fornes, M. T., Quinza, V., Colado, F., Arribas, B., Martinez, J. E. L., Garces, M. P., Valero, J. M., Frigola, J. L., Rubio, J. M., Gongora, A., Triana, J., Gomez, C., Garcia, M. L., Diez, C., Pombo, G., Gutierrez, M. C., Nazara, C. A., De Oca, M. A., Maquieira, N., Pilo, A., Buenadicha, J. L., Duran, F., Pena, J. E., Navarro, S., Serrano, F. J., Sanchez, S., Mesa, S., Salazar, J. A., De La Escosura, A., Perez, F., Solera, J., Tarraga, P., Ramos, L., Gutierrez, J. A., Caldelas, S., Albinana, F., Lopez, J. F., Florian, J., Rocha, E., Rodriguez, J., Mellado, M. L., Calvillo, M. R., Cruz, T., Capitan, M., Diaz, J., Tome, J., Prieto, A., Tome, V., Caro, M., Montero, A., Ontoria, M., Garcia, D. L., Gil, F., De Zarate, V., Casaponsa, J., Matamala, J., Salvande, A., Trueba, M. A., Cidra, J. L., Garcia, A., Toro, A., Berna, J. R., Zornoza, B., Prieto, M. T., Garcia, L., Lopez, C., Garcia, P., Echegaray, M. C., Navarro, B., Moro, M. L., Herrero, A., Tamargo, C., Perez, S. J., Antero, E., Penacho, M. C., Blanco, Y., Lucena, J., Sisto, C. A., Salido, E., Dolz, F., Granja, J. M., Saavedra, F., Sanchez-Oro, I., Mas-Magro, F., Artundo, T., Fernandez, A., Vaquer, J. V., Aso, K., Palacios, A., Morante, J. L., Seller, T., and Vendrell, J. M.
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Hypercholesterolemia ,Phytosterol ,Fermented milk ,Lifestyle ,Diet - Abstract
Introduction: RECIPE study was designed to assess the impact of dietary supplementation with fermented milk enriched with plant sterols (FMPS) on the nutritional behavior, lifestyle and the joint therapeutic responsibility of hypercholesterolemic patients. Methods: Observational, prospective, multicenter, international study, was conducted to assess dietary habits and other lifestyle components in hypercholesterolemic patients in whom the general practitioner indicated FMPS supplementation. Nutritional, lifestyle and clinical data were collected at the initial visit and after a 4 months follow-up. The biochemical and anthropometric data were retrieved from medical records obtained at baseline and 4 months. Results: We report the results of Spain. Two hundred and one physicians who provided valid data of 1044 patients participated. The addition of FMPS was associated with improved overall nutritional index, being adequate initially in 28.2% of patients, and increasing up to 75.2% at the end of the study (P< 001). This nutritional change was associated with an improvement in the lipid profile and anthropometric data. Patients more adherent to therapy generally achieved a better result in all parameters compared to non-compliant ones. Conclusions: The addition of FMPS to a diet designed to reduce the LDL enhances the patient's attitude regarding changes in lifestyle, leading to better overall control of dyslipidemia and anthropometric improvement. © 2012 Elsevier España, S.L. and SEA.
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- 2012
19. Ethinyl Estradiol-Cyproterone Acetate Versus Low Dose Pioglitazone-Flutamide Metformin for Adolescent Girls With Androgen Excess: Divergent Effects on CD 163, Tweak Receptor, ANGPTL4, and Leptin Expression in Subcutaneous Adipose Tissue EDITORIAL COMMENTS
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Díaz-Silva M, Chacon, M.R., Lopez-Bermejo, A., Maymo-Masip, E., Salvador, C., Vendrell, J., De Zegher, F., and Ibañez-Toda L
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- 2013
20. Gender determines the actions of adiponectin multimers on fetal growth and adiposity
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Megia, A., Ceperuelo-Mallafre, V., de la Flor, M., Vendrell, J., Ballesteros, M., Näf, S., Simón-Muela, I., Medicina i Cirurgia, and Universitat Rovira i Virgili.
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10.1016/j.ajog.2013.02.045
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- 2013
21. Evaluation of Health-Related Quality of Life according to Carbohydrate Metabolism Status: A Spanish Population-Based Study
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Vendrell, J., Vázquez, J.A., Valdés, S., Serrano-Rios, M., Rojo-Martínez, G., Pascual-Manich, G., Ortega, E., Mora-Peces, I., Menéndez, E., Martínez-Larrad, M.T., López-Alba, A., Gutiérrez, G., Gomis, R., Girbés, J., Gaztambide, S., Franch, J., Delgado, E., Catalá, M., Castell, C., Castaño, L., Casamitjana, R., Carmena, R., Bordiú, E., Bosch-Comas, A., Goday, A., Soriguer, F., Runkle, I., Fuentes, M., Calle-Pascual, A.L., Marcuello, C., Medicina i Cirurgia, and Universitat Rovira i Virgili.
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1687-8337 ,humanities - Abstract
10.1155/2012/872305 Objective. To evaluate the association between diabetes mellitus and health-related quality of life (HRQOL) controlled for several sociodemographic and anthropometric variables, in a representative sample of the Spanish population. Methods. A population-based, cross-sectional, and cluster sampling study, with the entire Spanish population as the target population. Five thousand and forty-seven participants (2162/2885 men/women) answered the HRQOL short form 12-questionnaire (SF-12). The physical (PCS-12) and the mental component summary (MCS-12) scores were assessed. Subjects were divided into four groups according to carbohydrate metabolism status: normal, prediabetes, unknown diabetes (UNKDM), and known diabetes (KDM). Logistic regression analyses were conducted. Results. Mean PCS-12/MCS-12 values were /, respectively. Men had higher scores than women in both PCS-12 ( versus ; ) and MCS-12 ( versus ; ). Increasing age and obesity were associated with a poorer PCS-12 score. In women lower PCS-12 and MCS-12 scores were associated with a higher level of glucose metabolism abnormality (prediabetes and diabetes), ( for trend), but only the PCS-12 score was associated with altered glucose levels in men ( for trend). The Odds Ratio adjusted for age, body mass index (BMI) and educational level, for a PCS-12 score below the median was 1.62 (CI 95%: 1.2–2.19; ) for men with KDM and 1.75 for women with KDM (CI 95%: 1.26–2.43; ), respectively. Conclusion. Current study indicates that increasing levels of altered carbohydrate metabolism are accompanied by a trend towards decreasing quality of life, mainly in women, in a representative sample of Spanish population.
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- 2012
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22. De novo lipogenesis in adipose tissue is associated with course of morbid obesity after bariatric surgery
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Garrido-Sánchez L., Vendrell J., Fernández-García D., Ceperuelo-Mallafré V., Chacón MR., Ocaña-Wilhelmi L., Alcaide J., Tinahones FJ., García-Fuentes E., Medicina i Cirurgia, and Universitat Rovira i Virgili.
- Abstract
10.1371/journal.pone.0031280 De novo lipogenesis is involved in fatty acid biosynthesis and could be involved in the regulation of the triglyceride storage capacity of adipose tissue. However, the association between lipogenic and lipolytic genes and the evolution of morbidly obese subjects after bariatric surgery remains unknown. In this prospective study we analyze the association between the improvement in the morbidly obese patients as a result of bariatric surgery and the basal expression of lipogenic and lipolytic genes.
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- 2012
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23. Plasma brain-derived neurotrophic factor in prepubertal obese children: Results from a 2-year lifestyle intervention programme
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Corripio R., Gónzalez-Clemente JM., Pérez-Sánchez J., Näf S., Gallart L., Vendrell J., Caixàs A., Bioquímica i Biotecnologia, Medicina i Cirurgia, and Universitat Rovira i Virgili.
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10.1111/j.1365-2265.2012.04431.x
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- 2012
24. Zinc-alpha 2-glycoprotein gene expression in adipose tissue is related with insulin resistance and lipolytic genes in morbidly obese patients
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Garrido-Sánchez L., García-Fuentes E., Fernández-García D., Escoté X., Alcaide J., Perez-Martinez P., Vendrell J., Tinahones FJ., Medicina i Cirurgia, and Universitat Rovira i Virgili.
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1932-6203 - Abstract
10.1371/journal.pone.0033264 Zinc-α2 glycoprotein (ZAG) stimulates lipid loss by adipocytes and may be involved in the regulation of adipose tissue metabolism. However, to date no studies have been made in the most extreme of obesity. The aims of this study are to analyze ZAG expression levels in adipose tissue from morbidly obese patients, and their relationship with lipogenic and lipolytic genes and with insulin resistance (IR).
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- 2012
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25. TNF-α inhibits PPARβ/δ activity and SIRT1 expression through NF-κB in human adipocytes
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Vázquez-Carrera, M., Vendrell, J., Palomer, X., Tinahones, F.J., Garrido-Sánchez, L., Wabistch, M., Salvadó, L., Barroso, E., Maymó-Masip, E., Chacón, M.R., Serrano-Marco, L., Bioquímica i Biotecnologia, Medicina i Cirurgia, and Universitat Rovira i Virgili.
- Abstract
10.1016/j.bbalip.2012.05.006 The mechanisms linking low-grade chronic inflammation with obesity-induced insulin resistance have only been partially elucidated. PPARß/d and SIRT1 might play a role in this association. In visceral adipose tissue (VAT) from obese insulin-resistant patients we observed enhanced p65 nuclear translocation and elevated expression of the pro-inflammatory cytokines TNF-a and IL-6 compared to control subjects. Inflammation was accompanied by a reduction in the levels of SIRT1 protein and an increase in PPARß/d mRNA levels. Stimulation of human mature SGBS adipocytes with TNF-a caused similar changes in PPARß/d and SIRT1 to those reported in obese patients. Unexpectedly, PPAR DNA-binding activity and the expression of PPARß/d-target genes was reduced following TNF-a stimulation, suggesting that the activity of this transcription factor was inhibited by cytokine treatment. Interestingly, the PPARß/d ligand GW501516 prevented the expression of inflammatory markers and the reduction in the expression of PPARß/d-target genes in adipocytes stimulated with TNF-a. Consistent with a role for NF-?B in the changes caused by TNF-a, treatment with the NF-?B inhibitor parthenolide restored PPAR DNA-binding activity, the expression of PPARß/d-target genes and the expression of SIRT1 and PPARß/d. These findings suggest that the reduction in PPARß/d activity and SIRT1 expression caused by TNF-a stimulation through NF-?B helps perpetuate the inflammatory process in human adipocytes
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- 2012
26. TNF-a inhibits PPARß/d activity and SIRT1 expression through NF-?B in human adipocytes
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Serrano-Marco L, Chacón MR, Maymó-Masip E, Barroso E, Salvadó L, Wabitsch M, Garrido-Sánchez L, Tinahones FJ, Palomer FX, Vendrell J, and Vazquez M
- Abstract
The mechanisms linking low-grade chronic inflammation with obesity-induced insulin resistance have only been partially elucidated. PPARß/d and SIRT1 might play a role in this association. In visceral adipose tissue (VAT) from obese insulin-resistant patients we observed enhanced p65 nuclear translocation and elevated expression of the pro-inflammatory cytokines TNF-a and IL-6 compared to control subjects. Inflammation was accompanied by a reduction in the levels of SIRT1 protein and an increase in PPARß/d mRNA levels. Stimulation of human mature SGBS adipocytes with TNF-a caused similar changes in PPARß/d and SIRT1 to those reported in obese patients. Unexpectedly, PPAR DNA-binding activity and the expression of PPARß/d-target genes was reduced following TNF-a stimulation, suggesting that the activity of this transcription factor was inhibited by cytokine treatment. Interestingly, the PPARß/d ligand GW501516 prevented the expression of inflammatory markers and the reduction in the expression of PPARß/d-target genes in adipocytes stimulated with TNF-a. Consistent with a role for NF-?B in the changes caused by TNF-a, treatment with the NF-?B inhibitor parthenolide restored PPAR DNA-binding activity, the expression of PPARß/d-target genes and the expression of SIRT1 and PPARß/d. These findings suggest that the reduction in PPARß/d activity and SIRT1 expression caused by TNF-a stimulation through NF-?B helps perpetuate the inflammatory process in human adipocytes.
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- 2012
27. Zinc alpha-2 glycoprotein is implicated in dyslipidaemia in HIV-1-infected patients treated with antiretroviral drugs
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Ceperuelo-Mallafré V., Escoté X., Viladés C., Peraire J., Domingo P., Solano E., Sirvent JJ., Pastor R., Tinahones F., Leal M., Richart C., Vendrell J., Vidal F., Medicina i Cirurgia, Ciències Mèdiques Bàsiques, and Universitat Rovira i Virgili.
- Abstract
10.1111/j.1468-1293.2011.00976.x Objectives: Treated HIV-1-infected patients with lipodystrophy often develop insulin resistance and proatherogenic dyslipidaemia. Zinc alpha-2 glycoprotein (ZAG) is a recently characterized adipokine which has been shown to be involved in the development of obesity and metabolic syndrome in uninfected subjects. We assessed the relationship between circulating ZAG levels and metabolic derangements in HIV-1-infected patients receiving antiretroviral drugs. Methods: Plasma ZAG levels were assessed in 222 individuals: 166 HIV-1-infected patients treated with antiretroviral drugs (77 with lipodystrophy and 89 without lipodystrophy) and 56 uninfected controls. Plasma ZAG levels were assessed by enzyme-linked immunosorbent assay (ELISA) and were correlated with fat distribution abnormalities and metabolic parameters. Results: HIV-1-infected patients had lower plasma ZAG levels compared with uninfected controls (P
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- 2012
28. Obesity-associated insulin resistance is correlated to adipose tissue vascular endothelial growth factors and metalloproteinase levels
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Tinahones FJ., Coín-Aragüez L., Mayas MD., Garcia-Fuentes E., Hurtado-Del-Pozo C., Vendrell J., Cardona F., Calvo RM., Obregon MJ., El Bekay R., Medicina i Cirurgia, and Universitat Rovira i Virgili.
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1472-6793 - Abstract
10.1186/1472-6793-12-4 The expansion of adipose tissue is linked to the development of its vasculature, which appears to have the potential to regulate the onset of obesity. However, at present, there are no studies highlighting the relationship between human adipose tissue angiogenesis and obesity-associated insulin resistance (IR).
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- 2012
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29. Zinc alpha-2 glycoprotein is implicated in dyslipidaemia in HIV-1-infected patients treated with antiretroviral drugs
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Ceperuelo-Mallafre, V, Escote, X, Vilades, C, Peraire, J, Domingo, P, Solano, E, Sirvent, JJ, Pastor, R, Tinahones, F, Leal, M, Richart, C, Vendrell, J, and Vidal, F
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dyslipidaemia ,lipodystrophy ,HIV ,zinc alpha-2 glycoprotein ,antiretroviral drugs ,metabolic syndrome - Abstract
Objectives Treated HIV-1-infected patients with lipodystrophy often develop insulin resistance and proatherogenic dyslipidaemia. Zinc alpha-2 glycoprotein (ZAG) is a recently characterized adipokine which has been shown to be involved in the development of obesity and metabolic syndrome in uninfected subjects. We assessed the relationship between circulating ZAG levels and metabolic derangements in HIV-1-infected patients receiving antiretroviral drugs. Methods Plasma ZAG levels were assessed in 222 individuals: 166 HIV-1-infected patients treated with antiretroviral drugs (77 with lipodystrophy and 89 without lipodystrophy) and 56 uninfected controls. Plasma ZAG levels were assessed by enzyme-linked immunosorbent assay (ELISA) and were correlated with fat distribution abnormalities and metabolic parameters. Results HIV-1-infected patients had lower plasma ZAG levels compared with uninfected controls (P < 0.001). No differences were found in ZAG plasma levels according to the presence of lipodystrophy, components of the metabolic syndrome or type of antiretroviral treatment regimen. Circulating ZAG levels were strongly determined by high-density lipoprotein cholesterol (HDLc) in men (B = 0.644; P < 0.001) and showed a positive correlation with total cholesterol (r = 0.312; P < 0.001) and HDLc (r = 0.216; P = 0.005). Conclusions HIV-1-infected patients have lower plasma ZAG levels than uninfected controls. In infected patients, plasma ZAG levels are in close relationship with total cholesterol and HDLc.
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- 2012
30. The usefulness of HbA1c in postpartum reclassification of gestational diabetes
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Megia A., Näf S., Herranz L., Serrat N., Yañez R., Simón I., Vendrell J., Medicina i Cirurgia, and Universitat Rovira i Virgili.
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10.1111/j.1471-0528.2012.03325.x
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- 2012
31. No Relationship Between TNF-alpha Genetic Variants and Combination Antiretroviral Therapy-Related Lipodystrophy Syndrome in HIV Type 1-Infected Patients: A Case-Control Study and a Meta-Analysis
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Veloso, S, Olona, M, Peraire, J, Vilades, C, Pardo, P, Domingo, P, Asensi, V, Broch, M, Aguilar, C, Lopez-Dupla, M, Aragones, G, Garcia-Pardo, G, Sirvent, JJ, Vendrell, J, Richart, C, and Vidal, F
- Abstract
Tumor necrosis factor alpha (TNF-alpha) is thought to be involved in the pathogenic and metabolic events associated with HIV-1 infection. We assessed whether carriage of the TNF-alpha gene promoter single nucleotide polymorphism (SNP) is associated with lipoclystrophy and metabolic derangements in HIV-1-infected patients treated with cART. We also assessed variations in TNF-alpha receptor plasma levels. The study group comprised 286 HIV-1-infected patients (133 with and 153 without lipodystrophy) and 203 uninfected controls (UC). TNF-alpha -238G > A, -308G > A, and -863 C > A SNP were assessed using PCR-RFLPs on white cell DNA. Plasma sTNF-alpha R1 and R2 levels were measured by ELISA. Student's t test, the chi(2) test, Pearson correlations, and the logistic regression test were performed for statistical analysis. The TNF-alpha -308G > A SNP was significantly associated with lipodystrophy in the univariate analysis (p = 0.04). This association, however, was no longer significant in the multivariate analysis. A meta-analysis of the published literature and our own data, which included 284 patients with lipodystrophy and 338 without lipodystrophy, showed that there was no relationship between the TNF-alpha -238G > A and -308G > A SNP and lipodystrophy (p > 0.05 for all comparisons). HIV-1-infected patients had greater sTNF-alpha R2 plasma levels than UC (p = 0.001) whereas sTNF-alpha R1 and R2 levels were not significantly different in both the HIV-1-infected cohorts, lipodystrophy vs. nonlipodystrophy (p = NS). In our cohort of white Spaniards the TNF-alpha -238G > A, -308G > A, and -863C > A SNP were not associated with lipodystrophy in HIV-1-infected patients treated with cART. This finding was replicated in a meta-analysis of the published data, which showed no associations between the TNF-alpha -238G > A and -308G > A SNP and lipodystrophy. In HIV-1-infected patients under cART there is a systemic overproduction of sTNF-alpha R2, which is unrelated to the presence of lipodystrophy.
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- 2011
32. Lipodystrophy and Insulin Resistance in Combination Antiretroviral Treated HIV-1-Infected Patients: Implication of Resistin
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Escote, X, Miranda, M, Veloso, S, Domingo, P, Alonso-Villaverde, C, Peraire, J, Vilades, C, Alba, V, Olona, M, Castro, A, Lopez-Dupla, M, Sirvent, JJ, Vicente, V, Vendrell, J, Richart, C, and Vidal, F
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lipodystrophy ,insulin resistance ,HIV ,combination antiretroviral therapy ,resistin - Abstract
Background: Little information is available with respect to the involvement of resistin in lipodystrophy and metabolic disturbances in HIV-1-infected patients treated with combination antiretroviral therapy (cART). We determined whether the resistin (rest) -420C>G single-nucleotide polymorphism and plasma resistin are associated with the development of lipodystrophy and metabolic disturbances in HIV-1-infected patients treated with cART. Methods: The study group comprised 299 HIV-1-infected patients treated with a stable cART for at least 1 year (143 with lipodystrophy and 156 without) and 175 uninfected controls. Anthropometric, clinical, and metabolic variables were determined. Homeostasis model assessment for insulin resistance was used to evaluate insulin resistance. Plasma resistin levels were determined by enzyme-linked immunosorbent assay. The rest -420>C. G was assessed using restriction fragment length polymorphism. Student t test, 1-way and 2-way analysis of variance, chi(2) test, and Pearson and Spearman correlations were performed for statistical analysis. Results: Genotypes containing the rest -420G variant allele were significantly more common in HIV-1-infected patients without lipodystrophy compared with those with lipodystrophy (P = 0.037). Infected patients had significantly greater plasma resistin levels than uninfected controls (P < 0.001). Among infected patients, plasma resistin levels were significantly lower in patients with lipodystrophy with respect to those without (P = 0.034). In infected patients, plasma resistin levels had a significant positive correlation with insulin and homeostasis model assessment for insulin resistance: P < 0.001 and P = 0.002 in the lipodystrophy subset and P = 0.002 and P = 0.03 in the nonlipodystrophy subset, respectively. Conclusions: In our cohort of white Spaniards, the rest -420>C. G single-nucleotide polymorphism may be associated with cART-related lipodystrophy. Plasma resistin correlates with insulin resistance in infected patients with and without lipodystrophy.
- Published
- 2011
33. A study of fatty acid binding protein 4 in HIV-1 infection and in combination antiretroviral therapy-related metabolic disturbances and lipodystrophy
- Author
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Escote, X, Megia, A, Lopez-Dupla, M, Miranda, M, Veloso, S, Alba, V, Domingo, P, Pardo, P, Vilades, C, Peraire, J, Giralt, M, Richart, C, Vendrell, J, and Vidal, F
- Subjects
subcutaneous adipose tissue ,dyslipidaemia ,lipodystrophy ,FABP-4 ,insulin resistance ,HIV-1 infection - Abstract
Objective The aim of the study was to determine circulating levels of fatty acid binding protein 4 (FABP-4) in a cohort of HIV-1-infected patients treated with combination antiretroviral therapy (cART) and to investigate the relationships between FABP-4 levels and insulin resistance, dyslipidaemia, lipodystrophy and levels of proinflammatory adipocytokines in these patients. Methods A total of 282 HIV-1-infected patients treated with stable cART for at least 1 year (132 with lipodystrophy and 150 without) and 185 uninfected controls (UCs) were included in the study. Anthropometric parameters were determined. Plasma levels of FABP-4, soluble tumour necrosis factor receptors 1 and 2 (sTNF-R1 and sTNF-R2), interleukin-18 (IL-18), IL-6, adiponectin and leptin were also analysed. Insulin resistance was determined using the homeostasis model assessment of insulin resistance (HOMA-IR). Subcutaneous adipose tissue mRNA expression of proinflammatory cytokines was assessed in 38 patients (25 with lipodystrophy and 13 without) by real-time polymerase chain reaction (PCR). Results The plasma FABP-4 concentration was significantly higher in patients with lipodystrophy than in those without (P = 0.012). FABP-4 concentration was positively correlated with body mass index (BMI), HOMA-IR, and the concentrations of insulin, total cholesterol, triglycerides, sTNF-R1, leptin and IL-18, but showed a negative correlation with high-density lipoprotein (HDL) cholesterol and adiponectin concentrations. After adjusting for age, sex and BMI, the odds ratio (OR) for risk of lipodystrophy was found to be significantly increased for those with the highest levels of FABP-4 [OR 0.838, 95% confidence interval (CI) 0.435-1.616 for medium FABP-4 vs. OR 2.281, 95% CI 1.163-4.475 for high FABP-4]. In a stepwise regression model, FABP-4 was independently associated with HOMA-IR after controlling for clinical and inflammatory parameters (P = 0.004). Moreover, a positive relationship was observed in patients with lipodystrophy between subcutaneous adipose tissue CD68 expression and FABP-4 plasma levels (r = 50.525; P = 50.031). Conclusions cART-treated HIV-1-infected patients with lipodystrophy have a systemic overproduction of FABP-4, which is closely linked to insulin resistance and inflammatory markers in subcutaneous adipose tissue.
- Published
- 2011
34. Determination of best tissues and culture media for microbiological confirmation of the serological results of the control campaigns of Brucella melitensis in sheep
- Author
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Álvarez, L, Durán, M, Gómez, B, Garrido, F, Yustes, C, Vendrell, J, Domínguez, L, Fernández-Garayzabal, JF, Vela, AI, and Latre, MV
- Subjects
medio Thayer Martin ,Thayer Martin medium ,Brucella melitensis ,brucelosis ,medio Farrell ,Farrell medium ,animal brucellosis - Abstract
El sacrificio de animales seropositivos para el control de la brucelosis ovina requiere del aislamiento microbiológico como técnica de confirmación del proceso. Debido a la dificultad de realización de las técnicas microbiológicas en un elevado número de animales, así como de la gran cantidad de muestras, es necesario establecer qué tejidos son los más adecuados para ello. No existen datos al respecto obtenidos de animales infectados naturalmente y representativos de las distintas situaciones epidemiológicas tal y como recomienda la Organización Internacional de Epizootias. En nuestro trabajo realizamos la necropsia de 92 animales sospechosos de infección brucelar efectuando posteriormente el cultivo e identificación microbiológica de 10 tejidos diferentes. Los animales fueron clasificados en función de una encuesta epidemiológica como pertenecientes a Rebaños con Brucelosis Crónica, Activa o de muy Baja Prevalencia. Los resultados muestran diferencias en función del tipo de rebaño, así como indican que la toma de muestras de mama y linfonódulos mamarios es la más adecuada para la confirmación de la infección en un rebaño sospechoso. El uso combinado de los medios de cultivo Farrell y Thayer Martin mostró una mayor eficacia según los resultados obtenidos por nosotros. Slaughtering seropositive animals in order to control ovine brucellosis requires a microbiological culture as a confirmatory technique. It is necessary to establish the most adequate tissues, due to difficulties involved in performing microbiological techniques in a large number of animals and samples. In this sense, there is a lack of data obtained from naturally infected sheep representatives of the different epidemiological situations, as recommended by OIE. In our study we carried out the necropsy of 92 ewes suspicious to be infected by Brucella and we performed microbiological culture and identification of 10 tissues per animal. Animals were classified according to an epidemiological survey as belonging to chronic brucellosis flocks, active brucellosis flocks and very low prevalence flocks. Results showed differences between flock types and also indicated that the analysis of samples from mammary gland and its lymph nodes was the best way to confirm infection in a suspicious flock. The combined use of Farrell and Thayer Martin media showed the best efficacy.
- Published
- 2010
35. PPAR gamma Pro12Ala Polymorphism in HIV-1-Infected Patients with HAART-Related Lipodystrophy
- Author
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Saumoy, M, Veloso, S, Alonso-Villaverde, C, Domingo, P, Chacon, MR, Miranda, M, Broch, M, Aragones, G, Gutierrez, MM, Vilades, C, Peraire, J, Sirvent, JJ, Lopez-Dupla, M, Aguilar, C, Auguet, T, Vendrell, J, Olona, M, Richart, C, and Vidal, F
- Subjects
PPAR gamma ,dyslipidaemia ,Lipodystrophy ,Pharmacogenetics ,HIV ,Polymorphism - Abstract
Peroxisome proliferator-activated receptor gamma (PPAR gamma) is involved in obesity and in some components of the metabolic syndrome in unselected population. To determine whether PPAR gamma genetic variants are associated with the risk of developing lipodystrophy and its associated metabolic disturbances in HIV-1-infected patients treated with HAART and to assess PPAR gamma mRNA expression in subcutaneous adipose tissue (SAT). The study group comprised 278 patients infected with HIV-1 and treated with antiretroviral drugs (139 with lipodystrophy and 139 without) and 105 uninfected controls (UC). The PPAR gamma Pro12Ala (C>G) single nucleotide polymorphism (SNP) was assessed using PCR-RFLPs on white cell DNA. PPAR gamma mRNA expression in SAT was assessed in 38 patients (25 with lipodystrophy and 13 without) and in 21 UC by real-time PCR. Statistical analysis was based on Student's T tests, chi(2) tests, Spearman's correlations tests and logistic regression tests. PPAR gamma Pro12Ala genotype distribution and allele frequencies were non-significantly different between both HIV-1-infected categories, lipodystrophy vs non-lipodystrophy (p = 0.9 and p = 0.87, respectively). Lipodystrophic patients harbouring the rare X/Ala genotype (Ala/Ala plus Pro/Ala) had significantly greater plasma total and LDL cholesterol levels compared with carriers of the common Pro/Pro genotype (p = 0.029 and p = 0.016, respectively) at univariate analyses. At multivariate analyses these associations were no longer significant. There was a near-significant decreased SAT PPAR gamma mRNA expression in patients with lipodystrophy compared to UC (p = 0.054). PPAR gamma Pro12Ala SNP has no effect on the risk of developing lipodystrophy in HIV-1-infected patients treated with HAART. PPAR gamma mRNA SAT expression appears decreased in lipodystrophy.
- Published
- 2009
36. IL6 gene promoter polymorphisms and type 2 diabetes: joint analysis of individual participants' data from 21 studies
- Author
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Huth, C., Heid, I. M., Vollmert, C., Gieger, C., Grallert, H., Wolford, J. K., Langer, B., Thorand, Barbara, Klopp, N., Hamid, Y. H., Pedersen, O., Hansen, T., Lyssenko, V., Groop, L., Meisinger, Christa, Doring, A., Löwel, H., Lieb, W., Hengstenberg, C., Rathmann, Wolfgang, Martin, S., Stephens, J. W., Ireland, H., Mather, H., Miller, G. J., Stringham, H. M., Boehnke, M., Tuomilehto, J., Boeing, H., Mohlig, M., Spranger, J., Pfeiffer, A., Wernstedt, I., Niklason, A., Lopez-Bermejo, A., Fernandez-Real, J.-M., Hanson, R. L., Gallart, L., Vendrell, J., Tsiavou, A., Hatziagelaki, E., Humphries, S. E., Wichmann, Heinz-Erich, Herder, C., and Illig, T.
- Subjects
Risk ,Genetics, Population ,Polymorphism, Genetic ,Diabetes Mellitus, Type 2 ,Interleukin-6 ,Case-Control Studies ,Odds Ratio ,Humans ,Promoter Regions, Genetic - Abstract
Several lines of evidence indicate a causal role of the cytokine interleukin (IL)-6 in the development of type 2 diabetes in humans. Two common polymorphisms in the promoter of the IL-6 encoding gene IL6, -174GC (rs1800795) and -573GC (rs1800796), have been investigated for association with type 2 diabetes in numerous studies but with results that have been largely equivocal. To clarify the relationship between the two IL6 variants and type 2 diabetes, we analyzed individual data on20,000 participants from 21 published and unpublished studies. Collected data represent eight different countries, making this the largest association analysis for type 2 diabetes reported to date. The GC and CC genotypes of IL6 -174GC were associated with a decreased risk of type 2 diabetes (odds ratio 0.91, P = 0.037), corresponding to a risk modification of nearly 9%. No evidence for association was found between IL6 -573GC and type 2 diabetes. The observed association of the IL6 -174 C-allele with a reduced risk of type 2 diabetes provides further evidence for the hypothesis that immune mediators are causally related to type 2 diabetes; however, because the association is borderline significant, additional data are still needed to confirm this finding.
- Published
- 2006
37. Reflexiones sobre la brucelosis de la oveja
- Author
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Durán Ferrer, Manuel, Gutiérrez Repiso, Carolina, Álvarez del Castillo, Lorenzo, Latre Cequiel, María Victoria, Garrido, Fulgencio, Yustes, C., Vendrell, J., Gómez, Belén, García López, María Jesús, Ruiz Villamor, Eduardo, Universitat Politècnica de Catalunya. Departament d'Enginyeria Agroalimentària i Biotecnologia, and Universitat Politècnica de Catalunya. ALIMen - Grup de recerca en Enginyeria i Producció Alimentària
- Subjects
Zoonosis ,Brucel·losi ovina ,Sheep--Diseases ,Ganado ovino ,Enginyeria agroalimentària::Ramaderia::Bestiar oví [Àrees temàtiques de la UPC] ,Brucelosis ,Bestiar oví -- Malalties ,Brucellosis in sheep - Published
- 2004
38. Gene expression profiling of hydroxy-tamoxifen resistance in breast cancer
- Author
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Vendrell, J., Duchesne, M., Chuchana, P., Pons, M., Nguyen, Cong Tu, Birnbaum, D., Nicolas, J., Theillet, C., Cohen, P., Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Science et Ingénierie des Matériaux et Procédés (SIMaP), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut de Chimie du CNRS (INC)-Institut National Polytechnique de Grenoble (INPG), Technologies avancées pour le génôme et la clinique (TAGC), and Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology - Published
- 2001
39. Gene expression profiling of hydroxy-tamoxifen resistance in breast cancer
- Author
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Vendrell, J., Duchesne, M., Chuchana, P., Pons, M., Nguyen, Cong Tu, Birnbaum, D., Nicolas, J., Theillet, C., Cohen, P., Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Science et Ingénierie des Matériaux et Procédés (SIMaP), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut de Chimie du CNRS (INC)-Institut National Polytechnique de Grenoble (INPG), Technologies avancées pour le génôme et la clinique (TAGC), and Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology - Published
- 2001
40. Gene expression profiling of hydroxy-tamoxifen resistance in breast cancer
- Author
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Vendrell, J., Duchesne, M., Chuchana, P., Pons, M., Nguyen, Cong Tu, Birnbaum, D., Nicolas, J., Theillet, C., Cohen, P., Institut de Génétique Moléculaire de Montpellier (IGMM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Science et Ingénierie des Matériaux et Procédés (SIMaP), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut National Polytechnique de Grenoble (INPG)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Technologies avancées pour le génôme et la clinique (TAGC), and Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology - Published
- 2001
41. Enginyeria de proteïnes: Complementarietat de les aproximacions experimentals i computacionals en el disseny de proteïnes
- Author
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Avilés Puigvert, Francesc Xavier, Oliva, Baldomero, Querol, E., Pérez-Pons, Josep Antoni, Canals, Francesc, Villegas, V., and Vendrell, J.
- Published
- 1998
42. [Apolipoprotein AI-CIII, B, and CII gene polymorphisms in patients with non-insulin dependent diabetes mellitus. Association with hyperlipemia]
- Author
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Gutiérrez C, Vendrell J, Broch M, Pastor R, Carl Llor, Simón I, and Richart C
- Subjects
Adult ,Male ,Polymorphism, Genetic ,Apolipoprotein A-I ,Diabetes Mellitus, Type 2 ,Risk Factors ,Humans ,Female ,Hyperlipidemias ,Middle Aged ,Apolipoproteins C ,Aged ,Apolipoproteins B - Abstract
Dislipidaemia is an usual feature in patients affected by non insulin dependent diabetes mellitus. Several studies show that this disease could be genetically determined. The aim of this study was to ascertain whether any of the genetic polymorphism remaining in three apolipoprotein loci (apolipoprotein AI-CIII, B100 and CII) is related with the presence of dislipidaemia in non insulin dependent diabetes mellitus patients.53 non insulin dependent diabetes mellitus patients with less than 5 years evolution and treated only with diet, were included. 86 healthy persons were included as the control group. The lipidic parameters analyzed were: cholesterol, LDL cholesterol, HDL cholesterol, triglycerides, apolipoprotein AI, B and lipoprotein (a). The following polymorphic variants were analyzed: RFLP-Sacl of the apolipoprotein AI-CIII-AIV cluster, RFLP-Xbal of the apolipoprotein B100 region and the RFLP-Taql of the apolipoprotein E-CI-CII cluster.There were no genetic nor allelic differences in the distribution of the genes, between controls and diabetic patients. Regarding the apolipoprotein CII gen, the diabetic patients with the T2T2 genotype had higher triglyceride levels (p0.01) compared with the remaining genotypes and compared with the control group having the same genotype (p0.01) matched for sex, age and body mass index. There was no difference in the metabolic parameters' distribution related to the genotypic distribution of the apolipoproteins AI-CIII and B100 genes.The apolipoprotein CII can be related with the presence of hypertriglyceridaemia in non insulin dependent diabetes mellitus patients.
- Published
- 1996
43. Calculation and Number Processing: Assessment Battery; Role of Demographic Factors
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Deloche, G. Larroque, C. Ferrand, I. Seron, X. Noel, M.N. Schils, J.P. Magnien, C. Metz-Lutz, M.N. Riva, I. Dordain, M. Baeta, E. Basso, A. Cipolotti, L. Claros-Salinas, D. Howard, D. Gaillard, F. Goldenberg, G. Mazzucchi, A. Stachowiai, F. Tzavaras, A. Vendrell, J. Bergego, C. Pradat-Diehl, P.
- Abstract
This paper describes the structure and contents of EC301, a standardized testing battery for the evaluation of brain-damaged adults in the area of calculation and number processing. The battery was administered to 180 normal subjects stratified by education (3 levels), age (3) and gender. EC301 is composed of a large variety of tasks dealing with basic arithmetic skills, and their linguistic, spatial, and mnesic dimensions. The three main notational systems for numbers - Arabic digits, written verbal, and spoken verbal number forms - are explored. Analysis of error rates indicated the effect of some demographic factors (principally, education; incidentally, gender) on normal performance in some tasks. © 1994, Taylor & Francis Group, LLC. All rights reserved.
- Published
- 1994
44. 40 EASD Annual Meeting of the European Association for the Study of Diabetes : Munich, Germany, 5-9 September 2004
- Author
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Veitenhansl, M., Stegner, K., Hierl, F-X, Dieterle, C., Feldmeier, H., Gutt, B., Landgraf, R., Garrow, A. P., Vileikyte, L., Findlow, A., Waterman, C., Boulton, A. J. M., Shankhdhar, K., Shankhdhar, L., Shankhdhar, U., Petrova, N. L., Foster, A. V. M., Edmonds, M. E., Ferraresi, R., Caravaggi, C., Giglio, R., Cavaiani, P., Pogliaghi, I., Sommariva, E., Katz, I. A., Harlan, A., Miranda-Palma, B., Prieto-Sanchez, L., Armstrong, D. G., Bowker, J. H., Mizel, M. S., Cernea, S., Wohlgelernter, J., Kidron, M., Modi, P., Raz, I., Arbit, E., Nosek, L., Kapitza, C., Beckett, P., Gelfand, R., Goldberg, M., Heise, T., Testa, M. A., Turner, R. R., Hayes, J. F., Scranton, R. E., Simonson, D. C., Yang, Y-W, Hsu, Y-J, Naujok, O., Francini, F., Jorns, A., Tiedge, M., Lenzen, S., Abdel-Wahab, Y. H. A., Marenah, L., Orr, D. F., Shaw, C., Flatt, P. R., Chokkalingam, K., Mansell, P. I., Clausen, P., Ekbom, P., Damm, P., Feldt-Rasmussen, U., Nielsen, B., Mathiesen, E. R., Feldt-Rasmussen, B., Dewan, S., Da Silva, N., Ternan, P. Mc, Leong, K. S., Wilding, J. P. H., Asatiani, N., Kurashvili, R., Dundua, M., Shelestova, E., Pagava, K., Ramazashvili, M., Hod, M., Smirnov, S., Petersen, J. L. A., Justesen, T. I., Ringholm Nielsen, L., Muller, C., Hojlund, K., Wensaas, A., Kase, E. T., Aas, V., Rustan, A. C., Thoresen, G. H., Levin, K., Beck-Nielsen, H., Gaster, M., Im, S-S, Kang, S-Y, Kim, S-Y, Ahn, Y-H, Lihn, A. S., Schmoll, D., Werner, T., Kienitz, A., Meyer, M., Barthel, A., Ailett, F., Sutherland, C., Walther, R., Grempler, R., Sasson, S., Reich, R., Tenenbaum, T., Alpert, E., Anfossi, G., Russo, I., Traversa, M., Massucco, P., Mattiello, L., Doronzo, G., Trovati, M., Lally, S., Tan, C. Y., Owens, D., Tomkin, G. H., Porchay, I., Pean, F., Bellili, N., Betoulle, D., Balkau, B., Tichet, J., Marre, M., Fumeron, F., Group D.E.S.I.R., Chatellier, G., Alhenc-Gelas, F., Diabhycar, Study Group, Nichols, G. A., Brown, J. B., Hayes, R. P., Bowman, L., Drexel, H., Saely, C. H., Marte, T., Benzer, W., Langer, P., Hoefle, G., Moll, W., Aczel, S., Karagiannis, E., Lubben, G., Urquhart, R., Edwards, G., Bruce, S., Howlett, H. S. C., Cugnardey, N., Turner, K. C., Park, J-S, Fiedorek, F. T., Avogaro, A., Gallo, A., Pinton, P., Rizzuto, R., Murphy, E., Ceolotto, G., Caterson, I., Guy-Grand, B., Hill, J., Barone, M., Aiello, A., Allochis, G., Borzi, V., Cannata, F., Caronna, S., D Avanzo, A., Elli, R., Formoso, G., Paroli, A., Scardapane, R., Sorichetti, P., Tatti, P., Viviani, G., Santeusanio, F., Italian Repaglinide Study Group, Manzella, D., Grella, R., Abbatecola, A. M., Paolisso, G., Sondergaard, L. G., Monster, T. B. M., Johnsen, S. P., Olsen, M. L., Mclaughlin, J. K., Sorensen, H. T., Lervang, H. H., Rungby, J., Lyssenko, V., Fredriksson, J., Almgren, P., Anevski, D., Orho-Melander, M., Sjogren, M., Tuomi, T., Groop, L., Jaziri, R., Aubert, R., Tuomilehto, J., Hu, G., Jousilahti, P., Peltonen, M., Lindstrom, J., Laina, A., Alevizaki, M., Philippou, G., Souvatzoglou, A., Anastasiou, E., Alba, S., Metcalf, B. S., Voss, L. D., Jeffery, A. N., Wilkin, T. J., Gluimer, C., Colagiuri, S., Vistisen, D., Borch-Johnsen, K., Haynes, A., Bower, C., Bulsara, M. K., Jones, T. W., Davis, E. A., Mortensen, H. B., Hougaard, P., Holl, R., Swift, P., Pociot, F., Knip, M., Hansen, L., Szadkowska, A., Pietrzak, I., Zmyslowska, A., Wyka, K., Bodalski, J., Holl, R. W., Swift, R., Hougaard, R., Gerstl, E-M, Engelsberger, I., Rabl, W., Rosenbauer, J., Grobe, H., Hofer, S. E., Krause, U., DPV-Wiss-Study Group, Dabelea, D., Morgan, T., Pettitt, D. J., Dolan, L., Mayer-Davis, E. J., Pihoker, C., Hillier, T. A., Imperatore, G., Ruggiero, A., Hamman, R. E., Stylianou, A., Tentolouris, N., Perrea, D., Tselepis, A. D., Lourida, E., Kitsou, E., Katsilambros, N., Vedovato, M., Dodesini, A. R., Lepore, G., Tiengo, A., Trevisan, R., Penno, G., Miccoli, R., Pucci, L., Lucchesi, D., Bandinelli, S., Fotino, C., Triscornia, S., Baldassari, E., Del Prato, S., Reboldi, P., Santeusanio, E., Fuller, J., Langham, R. G., Gow, R. M., Zhang, Y., Kelly, D. J., Christensen, P. K., Parving, H-H, Gilbert, R. E., Chibalin, A. V., Zhong, Z., Kotova, O., Davidescu, A., Ehren, I., Ekberg, K., Wahren, J., Wassef, L., Buckley, A. J., Rooney, K. B., Briody, J., Thompson, M., Ozanne, S. E., Thompson, C. H., Chamson-Reig, A., Summers, K., Arany, E. J. R., Hill, D. J., Solerte, S. B., Gazzaruso, C., Locatelli, E., Precerutti, S., Schifino, N., Ferrari, E., Fioravanti, M., Phenekos, C. V., Ginis, A., Fragaki, I., Chalkiadaki, M., Tzioras, C., Powell, L. A., Mcguire, G. M., Jewhurst, V., Trimble, E. R., Rasmussen, B. M., Vessby, B., Uusitupa, M., Berglund, L., Pedersen, E., Riccardi, G., Rivellese, A. A., Tapsell, L., Hermansen, K., Kanwu, Study Group, Da Silva Xavier, G., Rutter, J., Rutter, G. A., Briaud, I. M., Lingohr, M. K., Dickson, L. M., Mccuaig, J. R., Lawrence, J. C., Rhodes, C. J., Wikstrom, J. D., Katzman, S. M., Shirihai, O. S., Yang, J., Deng, S., Wang, X., Hessner, M. J., Wu, J., Wong, R. K., Sukumvanich, S., Markman, J. F., Naji, A., Wolf, B. A., Gao, Z., Rubi, B., Del Arco, A., Satrustegui, J., Maechler, P., Del Guerra, S., Lupi, R., Bugliani, M., Sbrana, S., Torri, S., Boggi, U., Vistoli, F., Mosca, F., Marchetti, P., Rennings, A. J. M., Smits, P., Stewart, M. W., Tack, C. J. J., Li, L., Nystrom, T., Gutniak, M., Ahren, B., Holst, J., Sjoholm, A., Gomes, M. B., Cailleaux, S., Tibirica, E., Albertini, J-P, Chen, H., Mather, R., Valensi, P. E., Chisalita, S. I., Arnqvist, H. 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45. Inventario del castillo de Altafulla (siglo XV)
- Author
-
Ricomá Vendrell, J. Javier
- Published
- 1978
46. Biological properties of a panel of murine monoclonal anti-Brucella antibodies
- Author
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Vendrell, J P, Delobbe, A, Huguet, M F, Peraldi, F, Serre, A, and Cannat, A
- Subjects
Mice ,Sepsis ,Complement Fixation Tests ,Animals ,Antibodies, Monoclonal ,Antibodies, Bacterial ,Brucella ,Brucellosis ,Spleen ,Research Article - Abstract
Immune sera have previously been shown to play a positive role in immune protection against murine experimental brucellosis. The protective properties of a panel of five anti-Brucella monoclonal antibodies (Mabs) were therefore assessed by estimation of the acceleration of the blood clearance of intravenously inoculated Brucella and of the reduction of splenic infection on Day 7 after infection. Three 'strongly protective', one 'weakly protective' and one 'non-protective' Mabs were identified. As a first step towards the study of the mechanism of this humoral protection, these Mabs were further compared for structural and functional properties such as immunoglobulin isotype, anti-Brucella specificity, anti-Brucella in vitro bacteriostasis, Brucella agglutination and complement fixation when complexed with tyndallized Brucella. No correlation was found between protection and either agglutination or direct bacteriostasis. On the other hand, the results observed suggest that isotypes (and especially the IgG2a isotype) could play an important role in in vivo immuno protection and that complement may be involved. However, the fact that one of the protective Mabs belongs to the IgA isotype, does not cross-react with the others in anti-Brucella epitopic specificity and does not fix complement underlines the probable diversity of the mechanisms involved.
- Published
- 1987
47. Surgical alternatives in renal transplantation with unavailability of the lower urinary tract
- Author
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Antonio Alcaraz Asensio, Talbot-Wright, R., Vilardell, J., Oppenheimer, F., Ricart, M. J., Barranco, M. A., Vendrell, J. R., and Carretero, P.
- Subjects
Adult ,Postoperative Complications ,Colon ,Anastomosis, Surgical ,Graft Survival ,Urinary Bladder ,Humans ,Kidney Failure, Chronic ,Child ,Kidney Transplantation ,Follow-Up Studies ,Retrospective Studies
48. Complement factor H is expressed in adipose tissue in association with insulin resistance
- Author
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Moreno-Navarrete, J., Martinez-Barricarte, R., Catalan, V., Sabater, M., Gomez-Ambrosi, J., Francisco José Ortega, Chacon, M. R., Ricart, W., Vendrell, J., Fruehbeck, G., Rodriguez Cordoba, S., and Fernandez-Real, J.
49. The TNF-α gene Nco I polymorphism influences the relationship among insulin resistance, percent body fat, and increased serum leptin levels
- Author
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Jose Manuel Fernández-Real, Gutierrez, C., Ricart, W., Casamitjana, R., Fernández-Castañer, M., Vendrell, J., Richart, C., and Soler, J.
50. Testicular sperm extraction in and intracytoplasmic sperm injection,RECUPERACLON DE ESPERMATOZOIDES DEL TEJIDO TESTICULAR Y MICROINYECCION ESPERMATICA EN EL CITOPLASMA DEL OVOCITO
- Author
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Calderon, G., Garcia, F., Belil, I., Vendrell, J. M., Begoña Aran, Coroleu, V., Veiga, A., and Barri, P. N.
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