1. The CHEK2*1100delC variant acts as a breast cancer risk modifier in non-BRCA1/BRCA2 multiple-case families
- Author
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Oldenburg, Ra, Kroeze-Jansema, K., Kraan, J., Morreau, H., Klijn, Jgm, nicoline hoogerbrugge, Ligtenberg, Mjl, Asperen, Cj, Vasen, Hfa, Meijers, C., Meijers-Heijboer, H., Bock, Th, Cornelisse, Cj, Devilee, P., Clinical Genetics, Medical Oncology, Internal Medicine, Pediatric Surgery, Damage and Repair in Cancer Development and Cancer Treatment (DARE), Life Course Epidemiology (LCE), and Human Genetics
- Subjects
P53 ,GENES ,MUTATIONS ,CHK2 ,SUSCEPTIBILITY ,TUMORS ,Tumor microenvironment [UMCN 1.3] ,REPEAT ,SDG 3 - Good Health and Well-being ,Genetic defects of metabolism [UMCN 5.1] ,HISTORY ,BRCA1 CARRIERS ,skin and connective tissue diseases ,CHEK2 ,Molecular diagnosis, prognosis and monitoring [UMCN 1.2] - Abstract
Item does not contain fulltext The frame-shifting mutation 1100delC in the cell-cycle-checkpoint kinase 2 gene (CHEK2) has been reported to be associated with familial breast cancer in families in which mutations in BRCA1 and BRCA2 were excluded. To investigate the role of this variant as a candidate breast cancer susceptibility allele, we determined its prevalence in 237 breast cancer patients and 331 healthy relatives derived from 71 non-BRCA1/BRCA2 multiple-case early onset breast cancer families. Twenty-seven patients (11.4%) were carrying the CHEK2*1100delC variant. At least one carrier was found in 15 of the 71 families (21.1%). There was no evidence of cosegregation between the variant and breast cancer, but carrier patients developed breast cancer earlier than did noncarriers. We studied CHEK2 protein expression in 111, and loss of heterozygosity at CHEK2 in 88 breast tumors from these patients. Twelve of 15 tumors from carriers showed absent protein expression as opposed to 3 of 76 tumors from noncarriers (P < 0.001). CHEK2 loss of heterozygosity was associated with absence of protein expression but not with 1100delC carrier status. Thus, selecting for breast cancer cases with a strong familial background not accounted for by BRCA1 or BRCA2 strongly enriches for carriers of CHEK2*1100delC. Our results support a model in which CHEK2*1100delC interacts with an as yet unknown gene (or genes) to increase breast cancer risk.
- Published
- 2003