1. em In vitro em drug interactions of gallates with antibiotics in em Staphylococcus Aureus em
- Author
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Meena Kishore Sakharkar, Win Mar Soe, Chu Sing Lim L, Tzer Pin Lin R, and Kishore R. Sakharkar
- Subjects
General Immunology and Microbiology ,Chemistry ,Tetracycline ,medicine.drug_class ,Fusidic acid ,Antibiotics ,Ethyl gallate ,Mupirocin ,medicine.disease_cause ,Antimicrobial ,General Biochemistry, Genetics and Molecular Biology ,Microbiology ,chemistry.chemical_compound ,Epicatechin gallate ,Staphylococcus aureus ,medicine ,lipids (amino acids, peptides, and proteins) ,medicine.drug - Abstract
The antimicrobial activities of tetracycline, mupirocin, and fusidic acid are tested in combination with Epicatechin Gallate (ECG), and Ethyl Gallate (EG) using 2 Methicillin resistant (MRSA) and 2 Methicillin sensitive (MSSA) strains of Staphylococcus aureus. Sub-inhibitory concentration of EG at 256 mg l-1 is found to be synergistic when used in combination with tetracycline, mupirocin, and fusidic acid; and a sub-inhibitory concentration of ECG at 32 mg l-1 is found to be synergistic with tetracycline in all the four Staphylococcus aureus strains tested. The synergistic combinations reduce the MICs of all the above three antibiotics by 4 fold. Combining ECG at 32 mg l-1 with mupirocin, reduces the MIC of mupirocin by four fold in MSSA C1 strain. 74 per cent of the combinations show consistent results in both time-kill assay and checkerboard method. The identified combinations may lead towards novel therapeutic interventions for treating MRSA infections.
- Published
- 2010
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