99 results on '"Tuo, Zhu"'
Search Results
2. Use of Deep Ultraviolet Emissive Carbon Dots as a Novel Optical Sensor for the Detection of Tetracyclines in Milk
- Author
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Wenzhi Yin, Jiao Gu, Tuo Zhu, Hui Gao, Chaoqun Ma, Chun Zhu, Lei Li, Zichen Yang, and Guoqing Chen
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Electrical and Electronic Engineering ,Instrumentation - Published
- 2022
3. Asymmetric Total Syntheses of Macroline-type Alkaloids Alstomicine and Alstofolinine A
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Kai Wei, Wen Hu, Yucui Sun, Tuo Zhu, Wen Chen, and Hongbin Zhang
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Organic Chemistry ,Catalysis - Abstract
Macroline-type monoterpenoid indole alkaloids represent a fascinating class of natural products with polycyclic structures, diverse biological and pharmacological activities. Herein we report the asymmetric total syntheses of two this type alkaloids, (–)-Alstomicine and (–)-Alstofolinine A. Notably, these two alkaloids are divergently synthesized from a common indole-fused azabicyclo[3.3.1]nonane intermediate, which was easily obtained via a Mannich-type cyclization. A SmI2 mediated Reformatsky reaction and a Pd-catalyzed carbonylative lactonization are employed to construct the lactone moieties presented in Alstomicine and Alstofolinine A respectively.
- Published
- 2023
4. Optimize classification testing of apple juice combining derivative fluorescence spectroscopy with PCA
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Chun Zhu, Xin-Shu Song, Huai-Bo Liu, Guo-Qing Chen, and Tuo Zhu
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Statistical and Nonlinear Physics ,Condensed Matter Physics - Abstract
Cluster analysis of apple juice is an important method in the quality identification of fruit juice. However, the traditional principal component analysis (PCA) is to reduce the dimension of high dimensional data and to achieve clustering by way of score value. Experiments have found that this clustering method is not suitable for real samples with fluorescence overlap. In this paper, we propose a processing mechanism to optimize the derivative of the fluorescence spectrum. When the derivative is calculated, we can amplify the spectral difference of the similar matter and improve the ability of the PCA to achieve the best effect of clustering. Experiments show that the optimized method for the actual sample classification accuracy rate of 100%, which can fully realize the type of commercially available apple juice beverage distinction and achieve the practical application of quality testing.
- Published
- 2022
5. EZH2 Regulates Intestinal Inflammation and Necroptosis Through the JNK Signaling Pathway in Intestinal Epithelial Cells
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Longgui Ning, Guo-Qiang Xu, Xinxin Zhou, Chunxiao Li, Hua-Tuo Zhu, Xinhe Lou, Sha Li, and Haojie Du
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MAP Kinase Signaling System ,Proto-Oncogene Proteins c-jun ,Physiology ,Necroptosis ,Down-Regulation ,macromolecular substances ,In Vitro Techniques ,Biology ,Real-Time Polymerase Chain Reaction ,Inflammatory bowel disease ,CCL5 ,Proinflammatory cytokine ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Crohn Disease ,Downregulation and upregulation ,medicine ,Animals ,Humans ,Enhancer of Zeste Homolog 2 Protein ,Intestinal Mucosa ,Chemokine CCL5 ,Inflammation ,Gene knockdown ,Chemokine CCL20 ,Tumor Necrosis Factor-alpha ,Dextran Sulfate ,Interleukin-17 ,Interleukin-8 ,EZH2 ,JNK Mitogen-Activated Protein Kinases ,Gastroenterology ,RNA-Binding Proteins ,Colitis ,Inflammatory Bowel Diseases ,Phosphoproteins ,medicine.disease ,Nuclear Pore Complex Proteins ,CCL20 ,Trinitrobenzenesulfonic Acid ,Gene Knockdown Techniques ,Receptor-Interacting Protein Serine-Threonine Kinases ,030220 oncology & carcinogenesis ,Cancer research ,Colitis, Ulcerative ,030211 gastroenterology & hepatology ,Caco-2 Cells - Abstract
Inflammatory bowel disease (IBD) is a common disorder of chronic intestinal inflammation that can be caused by the disruption of intestinal immune homeostasis. We aimed to evaluate the role of enhancer of zeste homolog 2 (EZH2) in the inflammatory response and explore the association between EZH2 and necroptosis in human epithelial colorectal adenocarcinoma cell lines. In both in vitro and in vivo models, expression of EZH2 in intestinal tissues was verified by histology. The expression of inflammatory cytokines in cell lines treated with EZH2 siRNA with or without stimulus was analyzed by quantitative real-time polymerase chain reaction. An intestinal necroptosis cell model was established to elucidate whether EZH2 is involved in necroptosis. Our present data indicated that EZH2 expression was decreased in in vitro and in vivo models and in patients with inflammatory bowel disease. EZH2 downregulation increased the expression of inflammatory factors, including TNF-α, IL-8, IL-17, CCL5, and CCL20 in a Caco-2 cell model. The JNK pathway was activated with the reduction of EZH2. In the necroptosis model, downregulation of EZH2 was detected with the upregulation of necroptotic markers RIP1 and RIP3. In addition, EZH2 knockdown with siRNA increased p-JNK and p-c-Jun. Our data suggest that EZH2 plays an important role in the development of intestinal inflammation and necroptosis. Hence, EZH2 could be a potential therapeutic target for IBD.
- Published
- 2019
6. Discrimination of Brands of Strong Aroma Type Liquors Using Synchronous Fluorescence Spectroscopy and Chemometrics Methods
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Guoqing Chen, Yamin Wu, Tuo Zhu, Zhuowei Zhu, and Yin Xu
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biology ,010401 analytical chemistry ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,biology.organism_classification ,Linear discriminant analysis ,01 natural sciences ,0104 chemical sciences ,Chemometrics ,Identification rate ,Support vector machine ,Principal component analysis ,0210 nano-technology ,Spectroscopy ,Biological system ,Aroma ,Mathematics ,Synchronous fluorescence - Abstract
The application of synchronous fluorescence spectroscopy combined with chemometrics using pretreated spectra was explored to develop a rapid, low-cost, and nondestructive method for discriminating between brands of different strong aroma type liquors. Principal component analysis, partial least square discriminant analysis, support vector machine, and back-propagation artificial neural network techniques were used to classify and predict the brands of liquor samples. Compared with the other models, the SVM model achieved the best results, with an identification rate of 100% for the calibration set, and 96.67% for the prediction set. The overall results showed that synchronous fluorescence spectroscopy with an efficient chemometrics method can be used successfully to identify different brands of liquor.
- Published
- 2019
7. Experimental and simulation studies of thermal transport based on plasma flow motion in laser-ablated dense regions of Au and CH
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Yuxue Zhang, Bo Qing, Yang Zhao, Tianming Song, Zhiyu Zhang, Gang Xiong, Chengwu Huang, Tuo Zhu, Min Lv, Yan Zhao, Jiyan Zhang, and Jiamin Yang
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Nuclear and High Energy Physics ,Nuclear Energy and Engineering ,Electrical and Electronic Engineering ,Atomic and Molecular Physics, and Optics - Abstract
A practical experimental method is proposed to investigate thermal transport by characterizing the motion of plasma flows through a x-ray spectroscopic technique using tracers. By simultaneously measuring multiple parameters, namely, the mass-ablation rate, the temporal evolution of plasma flow velocities and trajectories and the temperature, it is possible to observe a variety of physical processes, such as shock wave compression, heating by thermal waves, and plasma thermal expansion, and to determine their relative importance in different phases during the irradiation of CH and Au targets. From a comparison with hydrodynamic simulations, we find significant differences in the motion of the plasma flows between CH and Au, which can be attributed to different sensitivities to the thermal transport process. There are also differences in the ablation and electron temperature histories of the two materials. These results confirm that velocities and trajectories of plasma motion can provide useful evidence in the investigation of thermal conduction, and the approach presented here deserves more attention in the context of inertial confinement fusion and high-energy-density physics.
- Published
- 2022
8. Super-Twisting Sliding Mode Control of Brushless Excitation Synchronous Machine Drives
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Zhen Peng, Tuo Zhu, Zheng Li, YanYu Zhang, HaiXu Chen, and XingLai Peng
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Physics ,Rotary transformer ,Control theory ,Rotor (electric) ,law ,Torque ,Synchronous motor ,Field coil ,Sliding mode control ,law.invention ,Machine control - Abstract
This paper presents the brushless electrical excited synchronous machine drive system. A rotary transformer transfers the power to the rotor field winding of synchronous machine. A 12 slot - 10 pole brushless excited synchronous machine (BESM) is designed and analyzed. The super-twisting sliding mode direct torque and flux controller (STSM-DTC) is investigated for this motor drives. The tests show that STSM-DTC displays a very robust behavior.
- Published
- 2020
9. A Sliding Mode Observer for Sensorless Control of Fractional Slot Permanent Magnet Synchronous Motor
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Zheng Li, Tuo Zhu, HaiXu Chen, Zhen Peng, LvBao Guo, and JiaHui Xu
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Observer (quantum physics) ,Permanent magnet synchronous motor ,Computer science ,Control theory ,Mode (statistics) ,Synchronous motor ,Permanent magnet synchronous machine ,Finite element method ,Machine control - Abstract
This paper presents the sliding mode observer for sensorless control of 18 slot 14 pole permanent magnet synchronous motor (PMSM) drive system. The 18 slot 14 pole permanent magnet synchronous machine is designed and analyzed. The sliding mode observer (SMO) for sensorless controller is investigated for this motor drives. The tests show that the sliding mode observer for sensorless controller displays a very robust behavior.
- Published
- 2020
10. Inhibition of Histone Deacetylation by MS-275 Alleviates Colitis by Activating the Vitamin D Receptor
- Author
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Chaohui Yu, Yuwei Zhang, Longgui Ning, Z.X. Zhao, Jianzhong Sang, Lu Han, Jing Sun, Zhe Shen, Xiaochen Bo, Hua-Tuo Zhu, Xiuming Zhang, Bing Zhu, Weihua Zhou, Yi Chen, Lei Xu, Jinghua Wang, Youming Li, Min Zheng, Chunxiao Li, Zemin Feng, Chao Lu, and Xinhe Lou
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medicine.drug_class ,Pyridines ,Apoptosis ,Calcitriol receptor ,Inflammatory bowel disease ,Severity of Illness Index ,Histone Deacetylases ,Histones ,Histone H3 ,Mice ,medicine ,Animals ,Humans ,Colitis ,Intestinal Mucosa ,Histone H3 acetylation ,Cells, Cultured ,business.industry ,Histone deacetylase inhibitor ,Gastroenterology ,Acetylation ,General Medicine ,medicine.disease ,Vitamin D Deficiency ,Histone H3 deacetylation ,Histone Deacetylase Inhibitors ,Disease Models, Animal ,Vitamin D3 Receptor ,Benzamides ,Cancer research ,Receptors, Calcitriol ,Colitis, Ulcerative ,business ,Signal Transduction - Abstract
Background Ulcerative colitis [UC] is a common chronic inflammatory bowel disease without curative treatment. Methods We conducted gene set enrichment analysis to explore potential therapeutic agents for UC. Human colon tissue samples were collected to test H3 acetylation in UC. Both in vivo and in vitro colitis models were constructed to verify the role and mechanism of H3 acetylation modification in UC. Intestine-specific vitamin D receptor [VDR]-/- mice and VD [vitamin D]-deficient diet-fed mice were used to explore downstream molecular mechanisms accordingly. Results According to the Connectivity Map database, MS-275 [class I histone deacetylase inhibitor] was the top-ranked agent, indicating the potential importance of histone acetylation in the pathogenesis of UC. We then found that histone H3 acetylation was significantly lower in the colon epithelium of UC patients and negatively associated with disease severity. MS-275 treatment inhibited histone H3 deacetylation, subsequently attenuating nuclear factor kappa B [NF-κB]-induced inflammation, reducing cellular apoptosis, maintaining epithelial barrier function, and thereby reducing colitis activity in a mouse model of colitis. We also identified VDR as be a downstream effector of MS-275. The curative effect of MS-275 on colitis was abolished in VDR-/- mice and in VD-deficient diet-fed mice and VDR directly targeted p65. In UC patients, histone H3 acetylation, VDR and zonulin-1 expression showed similar downregulation patterns and were negatively associated with disease severity. Conclusions We demonstrate that MS-275 inhibits histone deacetylation and alleviates colitis by ameliorating inflammation, reducing apoptosis, and maintaining intestinal epithelial barrier via VDR, providing new strategies for UC treatment.
- Published
- 2020
11. Large heterotopic gastric mucosa and a concomitant diverticulum in the rectum: Clinical experience and endoscopic management
- Author
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Ming Yang, Lihua Chen, Guo-Qiang Xu, Hongtan Chen, Hua-Tuo Zhu, and Wenguo Chen
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Endoscopic ultrasound ,medicine.medical_specialty ,Adolescent ,Biopsy ,Perforation (oil well) ,Colonoscopy ,Rectum ,Case Report ,Choristoma ,Endosonography ,Lesion ,03 medical and health sciences ,Narrow Band Imaging ,0302 clinical medicine ,medicine ,Humans ,medicine.diagnostic_test ,Helicobacter pylori ,business.industry ,Heterotopic gastric mucosa ,Gastroenterology ,Endoscopy ,General Medicine ,medicine.disease ,Endoscopic submucosal dissection ,Hematochezia ,digestive system diseases ,Diverticulum ,medicine.anatomical_structure ,Rectal Diseases ,Gastric Mucosa ,030220 oncology & carcinogenesis ,030211 gastroenterology & hepatology ,Female ,Radiology ,medicine.symptom ,business ,Gastrointestinal Hemorrhage - Abstract
Heterotopic gastric mucosa (HGM) in the rectum is an extremely rare clinical entity which may be missed or misdiagnosed due to a lack of knowledge. In the present study, a 14-year-old girl visited our hospital due to a 5-year history of repeated hematochezia. Colonoscopy showed a solitary superficial depressed lesion approximately 5 cm in size and a concomitant 1.5 cm deep diverticulum in the rectum. Histological examination of the endoscopic biopsy showed typical ectopic gastric mucosa in the depressed lesion and inside the diverticulum. Narrow band imaging further confirmed the histological results. Endoscopic ultrasound indicated that the lesion originated from the mucosal layer, and partially involved the submucosal layer. Endoscopic submucosal dissection was performed in this patient due to the large size and shape of the lesion. No bleeding, perforation or other adverse events were observed. The presence of HGM in the diverticular cavity greatly increased the surgical difficulty. A literature review was also carried out in our study.
- Published
- 2018
12. Enhanced Mechanical Properties of a Low‐Carbon Martensitic Steel by Thermally Stable Ni‐Rich Austenite
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Zhaodong Wang, Tan Zhao, Tuo Zhu, Qinghai Wang, Hongtao Wang, Yongfeng Shen, Kan Liye, and Qibin Ye
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Austenite ,Materials science ,chemistry ,Martensite ,Metallurgy ,Materials Chemistry ,Metals and Alloys ,chemistry.chemical_element ,Physical and Theoretical Chemistry ,Condensed Matter Physics ,Carbon - Published
- 2021
13. Urea detection in milk by urease-assisted pH-sensitive carbon dots
- Author
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Guoqing Chen, Jiao Gu, Tingyu Wang, Lei Li, Ye Zhang, Wenzhi Yin, Chun Zhu, Tuo Zhu, Zichen Yang, and Chaoqun Ma
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Urease ,Fluorescence spectroscopy ,chemistry.chemical_compound ,Ammonia ,Hydrolysis ,Optics ,Quantum Dots ,Animals ,Urea ,Electrical and Electronic Engineering ,Engineering (miscellaneous) ,Fluorescent Dyes ,Detection limit ,Chromatography ,biology ,business.industry ,food and beverages ,Hydrogen-Ion Concentration ,Fluorescence ,Atomic and Molecular Physics, and Optics ,Milk ,Spectrometry, Fluorescence ,chemistry ,Carbon dioxide ,biology.protein ,business - Abstract
Excessive urea in milk will lead to serious health problems. To detect whether the urea concentration in milk exceeds the standard and ensure the quality of milk, it is necessary to develop detection technology for urea in milk. But it is difficult to detect urea in milk conveniently and accurately by traditional methods. To measure the concentration of urea in milk, stable green light carbon dots (CDs) were synthesized by a one-step method as a fluorescent probe. Then, 3, 5-diaminobenzoic acid was used as the precursor for CD synthesis. Experimental results showed that CDs can generate strong fluorescence when excited by light (350–450 nm). The fluorescence peak wavelength is 490 nm, and the optimum excitation wavelength is 390 nm. The fluorescence intensity of CDs has a significant change with variations of pH (pH of 6–9), and the higher the pH, the lower the fluorescence intensity. Additionally, urea can be hydrolyzed by urease to produce ammonia and carbon dioxide. Ammonia is ionized in water to produce O H − , which increases the pH of the solution. After adding standard urea to milk, urease and CDs are added. The fluorescence intensity of CDs in the mixed solution decreases as the concentration of standard added urea increases. Thus the concentration of urea in milk can be calculated. The experimental results show that the CD method for detecting urea in milk has advantages of high sensitivity and wide measurement range. The linear interval is 25–500 mg/L, R 2 is 0.998, and the limit of detection is 6.27 mg/L. The concentration of urea in the milk used in the experiment is 265.46 mg/L. CDs are easy to fabricate, and the advantages of the method are simple operation, no pretreatment, safety, and low cost. A new method for the detection of urea in milk was established, to the best of our knowledge, and this method can aid in food quality control.
- Published
- 2021
14. Multi-keV x-ray radiator from titanium cylindrical cavity at the Shenguang-III prototype laser facility
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Yi Tao, Lifei Hou, Dong Yang, Yukun Li, Chao Zeng, Yimeng Yang, Jiyan Zhang, Bo Qing, Longfei Jing, Jiamin Yang, Yuxue Zhang, Tianming Song, Xingsen Che, Junwen Gao, Yang Zhao, Yan Zhao, Tuo Zhu, Zheng Yuan, Ruizhen Yu, Xiayu Zan, Gang Xiong, Bo Yu, Guohong Yang, Minxi Wei, Zhimin Hu, Rongqing Yi, Yunsong Dong, Min Lv, Zhichao Li, Chengwu Huang, Hang Zhao, Kaiming Zhou, Zhiyu Zhang, and Jinghui Chen
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Physics ,Range (particle radiation) ,Thomson scattering ,business.industry ,Energy conversion efficiency ,Plasma ,Electron ,Photon energy ,Condensed Matter Physics ,Laser ,01 natural sciences ,010305 fluids & plasmas ,law.invention ,Optics ,law ,0103 physical sciences ,Electron temperature ,010306 general physics ,business - Abstract
Great efforts have been made to create a bright K-shell source using the thin metal cylindrical cavities in the past few decades. Several metal materials such as titanium (Ti), iron, and so on have been tested for high x-ray conversion efficiency mainly at the OMEGA and NIF laser facilities. Recently, x-ray sources in Ti K-shell transition energy range were investigated at the Shenguang-III prototype laser facility with ∼5 kJ laser energy. The experiments were aimed to reproduce the previous ones at OMEGA, but with an extraordinarily small volume of cylindrical cavity and detailed characterizations of the x-ray source. The cavities were 800 μm inner diameter, 800 μm length, and 30 μm thick plastic tubes supporting 1 μm thick Ti. Seven laser beams were focused to 200 μm diameter. The combination of the small cavity volume and the focused laser spots is intended to improve the electron temperature with limited laser energy since the electron temperature is a key issue for high x-ray conversion efficiency. Thomson scattering was adopted to experimentally probe the electron temperatures at special time and space zones as well as the average temperature obtained from the Ti K-shell spectrum. The evolutions of the electron temperature and density are predicted by the radiation hydrodynamic simulation. A top view and two photon energy bands of x-ray source images provide a way to directly observe the plasma movement toward the cavity axis and distinguish the different emission mechanisms between the Ti K-shell and lower energy x rays. Six Higher-energy x-ray detectors located at different angles were used to record the Ti K-shell x-ray emission and demonstrate its isotropic feature. The characteristics of the x-ray radiate intensity including the time evolution, the angular distribution, and the total yields for both the photon energy regions above and below 4 keV are compared between the cavity and planar targets. Obviously, different behaviors were found between the two photon energy regions and the two types of targets. The x-ray conversion efficiency of the Ti cylinder was determined to be ∼ 4 % and ∼ 21 % in 4 π sr in the Ti K-shell (4–7 keV) and
- Published
- 2021
15. Continued Study on Hohlraum Radiation Source with Approximately Constant Radiation Temperature
- Author
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Jiamin Yang, Zhichao Li, Tianming Song, Tuo Zhu, and Chengwu Huang
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Physics ,business.industry ,medicine.medical_treatment ,Plasma ,Radiation ,Condensed Matter Physics ,Laser ,Ablation ,01 natural sciences ,010305 fluids & plasmas ,law.invention ,Pulse (physics) ,Optics ,Hohlraum ,law ,Power Balance ,0103 physical sciences ,medicine ,010306 general physics ,business ,Flattop - Abstract
An experiment was performed on the Shenguang III prototype laser facility to continue the study on hohlraum radiation source with approximately constant radiation temperature using a continuously shaped laser pulse. A radiation source with a flattop temperature of about 130 eV that lasted about 5 ns was obtained. The previous analytical iteration method based on power balance and self-similar solution of ablation was modified taking into account the plasma movements and it was used to design the laser pulse shape for experiment. A comparison between experimental results and simulation is presented and better agreement was achieved using the modified method. Further improvements are discussed.
- Published
- 2016
16. Corrigendum to: Inhibition of Histone Deacetylation by MS-275 Alleviates Colitis by Activating the Vitamin D Receptor
- Author
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Hua-Tuo Zhu, Jianzhong Sang, Xiaochen Bo, Chaohui Yu, Longgui Ning, Lu Han, Zhe Shen, Jing Sun, Z.X. Zhao, Zemin Feng, Chao Lu, Xinhe Lou, Xiuming Zhang, Bing Zhu, Yuwei Zhang, Yi Chen, Lei Xu, Jinghua Wang, Weihua Zhou, Chunxiao Li, Youming Li, and Min Zheng
- Subjects
biology ,business.industry ,Gastroenterology ,General Medicine ,Pharmacology ,medicine.disease ,Calcitriol receptor ,Text mining ,Histone ,Acetylation ,biology.protein ,medicine ,Colitis ,business - Published
- 2020
17. [The Study of Three-Dimensional Synchronous Fluorescence Spectral Characteristics of Liuwei Dihuang Pills]
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Chun, Zhu, Xin, Xie, Jin-chen, Zhao, Jia-meng, Du, Guo-qing, Chen, and Tuo, Zhu
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Spectrometry, Fluorescence ,Drugs, Chinese Herbal - Abstract
Three-dimensional synchronous fluorescence of each component of Liuwei dihuang pills (Prepared rehmannia root, cornel, yam, alisma, poria cocos, and cortex moutan) is measured by using FLS920P fluorescence spectrometer. Feature parameters were extracted. It can be found that each component is fluorescent material and the lines are all different. Furthermore, Three-dimensional synchronous fluorescence of Liuwei dihuang pills boiling with standard water and non-standard water are all measured and there are significant differences between them. It can be applied in distinguishing different formula of Chinese medicine decoction. Experimental and Theoretical conclusion show that: the three-dimensional fluorescence spectrometry method and the combination of synchronous fluorescence spectroscopy method can further improve the sensitivity and selectivity of fluorescence spectroscopy, have a distinct advantage in a complex multi-component mixture of fluorescence spectroscopic analysis. The study can provide a convenient and reliable method for establishing a complete fingerprint of Chinese traditional medicine. It also can help identifying the component and the quality of Chinese patent medicine.
- Published
- 2018
18. Dysregulated Up-Frameshift Protein 1 Promotes Ulcerative Colitis Pathogenesis Through the TNFR1-NF-κB/MAPKs Pathway
- Author
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Guo-Qiang Xu, Min Yue, Lihua Chen, Guodong Shan, Hongtan Chen, Hua-Tuo Zhu, Xiaowei Xu, Chao Lu, Chunxiao Li, Xinhe Lou, Shujun Huang, and Wenguo Chen
- Subjects
0301 basic medicine ,Physiology ,MAP Kinase Signaling System ,p38 mitogen-activated protein kinases ,IκB kinase ,Pathogenesis ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Downregulation and upregulation ,medicine ,Animals ,Colitis ,Intestinal Mucosa ,Inflammation ,Chemistry ,Gastroenterology ,NF-kappa B ,NF-κB ,Zinc Fingers ,medicine.disease ,Immunohistochemistry ,Blot ,Disease Models, Animal ,030104 developmental biology ,Gene Expression Regulation ,Receptors, Tumor Necrosis Factor, Type I ,030220 oncology & carcinogenesis ,Cancer research ,Trans-Activators ,Colitis, Ulcerative ,Tumor necrosis factor receptor 1 ,Signal Transduction - Abstract
Ulcerative colitis (UC) is an idiopathic colonic mucosal disease, and its pathogenesis has not been fully understood. Up-frameshift protein 1 (UPF1) is a potential molecule for UC predicted by a computational approach. The present study aimed to validate the underlying mechanism of UPF1 in UC. UPF1 expression was detected by qRT-PCR, western blotting, and immunohistochemistry in dextran sulfate sodium-induced colitis in mice. To simulate the intestinal inflammation microenvironment, NCM460 human colonic epithelial cells were exposed to a mixture of inflammatory mediators. The potential mechanism involving TNFR1-NF-κB/MAPKs pathway activation was addressed by western blotting, reporter gene assays, and siRNA (siUPF1) or UPF1-expressing plasmid pENTER-transfected cells. UPF1 was downregulated in colonic epithelial cells of colitic mice, and in vitro, contrary to the mRNA levels of the associated cytokines enhanced in the UPF1 dysregulation group within stimulatory factors, most relevant cytokines were significantly decreased in UPF1 overexpression group. Mechanistically, the increased expression of tumor necrosis factor receptor 1 (TNFR1) was found in NCM460 cells pre-treated with siUPF1, with the activation of IKK/NF-κB and MAPKs pathways, including JNK/AP-1 and P38, but not the ERK1/2 pathway. Moreover, the repression of TNFR1 required the interaction of UPF1 with the promoter. UPF1, which negatively regulated the transcription of TNFR1, is a novel factor regulating intestinal inflammation. The downregulation of UPF1 activated the TNFR1-dependent NF-κB/MAPKs pathway, and promoting inflammatory responses in colon might act as a causal role in UC.
- Published
- 2018
19. Engineering Photosystem I Complexes with Metal Oxide Binding Peptides for Bioelectronic Applications
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Lijia Wang, Barry D. Bruce, Richard F. Simmerman, Tuo Zhu, Sanjay R. Mishra, David R. Baker, and Cynthia A. Lundgren
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Auxiliary electrode ,Circular dichroism ,Bioelectric Energy Sources ,Recombinant Fusion Proteins ,Blotting, Western ,Biomedical Engineering ,Oxide ,Pharmaceutical Science ,chemistry.chemical_element ,Bioengineering ,Nanotechnology ,Protein Engineering ,Photosystem I ,Metal ,chemistry.chemical_compound ,Escherichia coli ,Ferredoxin ,Titanium ,Pharmacology ,Photosystem I Protein Complex ,Chemistry ,Circular Dichroism ,Organic Chemistry ,Combinatorial chemistry ,Semiconductors ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,visual_art ,visual_art.visual_art_medium ,Ferredoxins ,Nanoparticles ,Zinc Oxide ,Cyclic voltammetry ,Peptides ,Platinum ,Biotechnology - Abstract
Conventional dye-sensitized solar cells comprise semiconducting anodes sensitized with complex synthetic organometallic dyes, a platinum counter electrode, and a liquid electrolyte. This work focuses on replacing synthetic dyes with a naturally occurring biological pigment-protein complex known as Photosystem I (PSI). Specifically, ZnO binding peptides (ZOBiP)-fused PSI subunits (ZOBiP-PsaD and ZOBiP-PsaE) and TiO2 binding peptides (TOBiP)-fused ferredoxin (TOBiP-Fd) have been produced recombinantly from Escherichia coli. The MOBiP-fused peptides have been characterized via western blotting, circular dichroism, MALDI-TOF, and cyclic voltammetry. ZOBiP-PSI subunits have been used to replace wild-type PsaD and PsaE, and TOBiP-Fd has been chemically cross-linked to the stromal hump of PSI. These MOBiP peptides and MOBiP-PSI complexes have been produced and incubated with various metal oxide nanoparticles, showing increased binding when compared to that of wild-type PSI complexes.
- Published
- 2015
20. Molecular interactions between photosystem I and ferredoxin: an integrated energy frustration and experimental model
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Richard F. Simmerman, Jerome Baudry, Tuo Zhu, Barry D. Bruce, Cathy Scott, and Derek J. Cashman
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Molecular model ,biology ,Flavodoxin ,Chemistry ,media_common.quotation_subject ,Frustration ,Energy landscape ,Photosystem I ,Electron transfer ,Crystallography ,Structural Biology ,Docking (molecular) ,biology.protein ,Biophysics ,Molecular Biology ,Ferredoxin ,media_common - Abstract
The stromal domain (PsaC, PsaD, and PsaE) of photosystem I (PSI) reduces transiently bound ferredoxin (Fd) or flavodoxin. Experimental structures exist for all of these protein partners individually, but no experimental structure of the PSI/Fd or PSI/flavodoxin complexes is presently available. Molecular models of Fd docked onto the stromal domain of the cyanobacterial PSI site are constructed here utilizing X-ray and NMR structures of PSI and Fd, respectively. Predictions of potential protein-protein interaction regions are based on experimental site-directed mutagenesis and cross-linking studies to guide rigid body docking calculations of Fd into PSI, complemented by energy landscape theory to bring together regions of high energetic frustration on each of the interacting proteins. The results identify two regions of high localized frustration on the surface of Fd that contain negatively charged Asp and Glu residues. This study predicts that these regions interact predominantly with regions of high localized frustration on the PsaC, PsaD, and PsaE chains of PSI, which include several residues predicted by previous experimental studies. Copyright © 2014 John Wiley & Sons, Ltd. Additional supporting information may be found in the online version of this article at the publisher’s web site.
- Published
- 2014
21. Effectiveness of mesalazine to treat irritable bowel syndrome
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Hua-Tuo Zhu, Guo-Qiang Xu, Liang Ding, Sha Li, Fenming Zhang, Sai-Heng Xiang, and Jinghua Yu
- Subjects
medicine.medical_specialty ,Abdominal pain ,Cochrane Library ,Placebo ,Gastroenterology ,03 medical and health sciences ,chemistry.chemical_compound ,immune cells ,0302 clinical medicine ,Gastrointestinal Agents ,Mesalazine ,Internal medicine ,medicine ,Humans ,Treatment Failure ,adverse reactions ,030212 general & internal medicine ,Mesalamine ,Irritable bowel syndrome ,clinical symptoms ,irritable bowel syndrome ,business.industry ,General Medicine ,medicine.disease ,Confidence interval ,meta-analysis ,chemistry ,mesalazine ,030220 oncology & carcinogenesis ,Meta-analysis ,Defecation ,medicine.symptom ,business ,Systematic Review and Meta-Analysis ,Research Article - Abstract
Aim: Accumulating evidence has explored the effect of mesalazine on irritable bowel syndrome (IBS). However, these studies remain inconsistent. Thus, a meta-analysis was conducted to estimate the role of mesalazine on IBS. Methods: PubMed, Medline, Embase, Web of Science, and the Cochrane Library Database were searched for all relevant randomized, controlled, blinded trials on mesalazine in patients with IBS between January 1980 and October 2018. All statistical analyses were performed using Revman 5.3 software. A fixed-effects model was adopted, 95% confidence intervals for SMD was calculated. Heterogeneity was evaluated by χ2 test and I2 statistic. Results: Five studies involving 387 participants were finally included in this meta-analysis. The results showed that the SMD for clinical efficacy on abdominal pain in IBS patients treated with mesalazine in comparison to placebo was 0.19 (95% CI = −0.01 to 0.39, P = .06), which was statistically non-significant but clinically important. For beneficial effect of abdominal bloating, the SMD was 0.05 (95% CI = −0.20 to 0.30, P = .70), which was statistically non-significant. In regard to clinical efficacy on defecation frequency per day, the results revealed that the SMD was 0.29 (95% CI = −0.14 to 0.73, P = .18), which was statistically non-significant but clinically important. As for beneficial effect of general well-being, we found that the SMD was 0.41 (95% CI = −0.75 to 1.58, P = .49), which was statistically non-significant. With respect to stool consistency, the SMD was 0.01 (95% CI = −0.31 to 0.33, P = .96), which was statistically non-significant. For the effect of defecation urgency severity in IBS patients treated with mesalazine in comparison to placebo, we detected a surprising result with an SMD of 0.54 (95% CI = 0.05–1.04, P = .03), which was statistically significant. There was no significant difference between mesalazine group and placebo group on total mucosal immune cell counts of the patients with IBS with an SMD of −1.64 (95% CI = −6.17 to 2.89, P = .48) and there was also no significant difference in adverse reactions between two groups with an SMD of 1.05 (95% CI = 0.76–1.46 P = .77). Conclusion: Mesalazine is not superior to placebo in relieving clinical symptoms of abdominal pain, abdominal bloating, and general well-being of IBS and has no advantage of reducing defecation frequency per day and immune cell infiltration and improving stool consistency though without adverse reactions of mesalazine compared with placebo. For defecation urgency severity, placebo is even superior to mesalazine for IBS patients. Thus, mesalazine might be a cost burden to patients without providing good effectiveness. In view of the small sample size of the current study and the differences in every experimental designs, this study has high heterogeneity and requires subsequent verification.
- Published
- 2019
22. Evaluation of coal swelling-controlled CO2diffusion processes
- Author
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Wei Li, Yuanping Cheng, Shouqing Lu, and Jin-tuo Zhu
- Subjects
Environmental Engineering ,Chemistry ,business.industry ,Analytical chemistry ,Coal mining ,Thermodynamics ,complex mixtures ,Desorption ,medicine ,Environmental Chemistry ,Gaseous diffusion ,Coal ,Astrophysics::Earth and Planetary Astrophysics ,Diffusion (business) ,Swelling ,medicine.symptom ,business ,Glass transition ,Mass fraction ,Physics::Atmospheric and Oceanic Physics - Abstract
CO2 sequestration in deep and unmineable coalbeds is regarded as a viable option for carbon storage. CO2 diffusion is a key parameter determining the effectiveness of sealing CO2 in coal seams. By conducting laboratory tests of coal CO2 desorption and unsteady gas diffusion theory model calculations, the desorption dynamics of Yaojie coal were studied under the condition of constant temperature and varied balance pressures, and the CO2 diffusion coefficient was obtained using the non-steady diffusion model at different times. The results show that the higher the balance pressure is, the greater the diffusion coefficient is under the same conditions. As the pressure drops, the CO2 diffusion coefficient gradually decreases as time increases, and the mass fraction of desorbed CO2 has a linear relationship with the diffusion coefficient. It was observed that under high pressures, the diffusion coefficient initially increases and then gradually decreases. The non-steady CO2 diffusion in Yaojie coal is related to CO2-induced swelling and to the effect of dissolved CO2 on the coal's structures and properties, especially under high-pressure conditions. The glass transition of coal containing CO2 may be the governing factor leading to the nonlinear diffusion of CO2 in coal.
- Published
- 2013
23. Helicobacter pyloriInduces Increased Expression of the Vitamin D Receptor in Immune Responses
- Author
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Yu Chen, Chaohui Yu, Wenguo Chen, Xingyong Wan, Shuhua Xu, Hua-Tuo Zhu, Lihua Chen, Ningmin Yang, and Lihua Guo
- Subjects
Adult ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Inflammation ,Real-Time Polymerase Chain Reaction ,Severity of Illness Index ,Calcitriol receptor ,Cell Line ,Helicobacter Infections ,Cathelicidin ,Young Adult ,Immune system ,Internal medicine ,medicine ,Gastric mucosa ,Humans ,Prospective Studies ,Interleukin 8 ,Aged ,Innate immune system ,Helicobacter pylori ,biology ,Histocytochemistry ,Gastroenterology ,Epithelial Cells ,General Medicine ,Middle Aged ,biology.organism_classification ,Immunity, Innate ,Infectious Diseases ,medicine.anatomical_structure ,Endocrinology ,Gastric Mucosa ,Host-Pathogen Interactions ,Receptors, Calcitriol ,Female ,medicine.symptom - Abstract
Background: Vitamin D receptor (VDR) is a member of the nuclear receptor family of transcription factors that play a critical role in innate immunity. This study examined the role of VDR in gastric innate immune defence against the gastric pathogen Helicobacter pylori. Materials and Methods: Seventeen H. pylori-infected patients and sixteen controls participated in the study. The GES-1 cells were transfected with siRNA or incubated with or without 1a,25(OH)2D3 (100 nmol/L) then infected with H. pylori. VDR, cathelicidin antimicrobial protein (CAMP), and cytokine mRNA expression levels in normal and H. pylori-infected gastric mucosa and GES-1 cells was determined by qRT-PCR and correlated with the histopathologic degree of gastritis. Bactericidal activity was measured by using a colony-forming unit assay. Results: Vitamin D receptor mRNA expression levels were significantly upregulated in H. pylori-infected patients and positively correlated with chronic inflammation scores. There was a significant positive correlation between VDR and CAMP mRNA expression in H. pylori-positive gastric mucosa. VDR siRNA reduced H. pylori-induced CAMP production and conversely increased IL-6 and IL8/CXCL8 expression levels. The vitamin D agonist 1a,25(OH)2D3 increased CAMP expression and reduced cytokine activation in GES-1 cells infected with H. pylori .1 a,25(OH)2D3 could enhance the intracellular killing of the replicating bacteria, but the presence of siVDR and siCAMP led to a decline in its bactericidal ability. Conclusions: The expression of VDR and CAMP in the gastric epithelium is up-regulated in the case of H. pylori infection; thus, VDR plays an important role in gastric mucosa homeostasis and host protection from H. pylori infection.
- Published
- 2013
24. Experimental study of X-ray emission properties of laser produced plasmas with Au and Au foam layer targets
- Author
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Tuo Zhu, Wanli Shang, Tianming Song, Chenwu Huang, Wenhai Zhang, Yunsong Dong, and Jiamin Yang
- Subjects
Materials science ,Spectral power distribution ,Astrophysics::High Energy Astrophysical Phenomena ,Mechanical Engineering ,Energy conversion efficiency ,Metals and Alloys ,X-ray ,Plasma ,Laser ,law.invention ,Angular distribution ,Mechanics of Materials ,law ,Materials Chemistry ,Atomic physics ,Layer (electronics) - Abstract
The X-ray emission properties of Au plasmas consist of spectral distribution, M band fraction (2–4 keV), X-ray angular distribution and X-ray conversion efficiency, are imperative for various X-ray source applications. In order to investigate the X-ray emission properties of laser produced plasmas with Au and Au foam targets, experiments were carried out in Shenguang II laser facility. Au foam targets with densities of 0.6 and 0.4 g/cm3 were employed. The X-ray emission properties were measured. Excellent agreements of the experimental and theoretical spectral distributions and M band fractions were achieved.
- Published
- 2013
25. Determination of Amaranth in cocktail by synchronous three-dimensional fluorescence spectrometry
- Author
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Guoqing Chen, Chun Zhu, Cong-Hai Zhu, Xin-Shu Song, and Tuo Zhu
- Subjects
Materials science ,010401 analytical chemistry ,Three dimensional fluorescence ,Analytical chemistry ,Statistical and Nonlinear Physics ,Amaranth ,010501 environmental sciences ,Condensed Matter Physics ,Mass spectrometry ,01 natural sciences ,Fluorescence spectra ,0104 chemical sciences ,Correction algorithm ,chemistry.chemical_compound ,chemistry ,0105 earth and related environmental sciences - Abstract
Three-dimensional (3D) fluorescence spectra and synchronous 3D fluorescence spectra are used to analyze the concentration of Amaranth in commercial cocktails by Parallel Factor Correction Algorithm. The former average relative error of the Amaranth in the commercial cocktail is 15%, and the latter average relative error is less than 4.0%. The results show that the synchronous fluorescence spectrum can improve the sensitivity and selectivity of the fluorescence spectroscopy technique greatly, and it has obvious advantages on analysis of the complex multi-components system. Finally, the content of Amaranth in the cocktail (about [Formula: see text]) is much lower than that of the national standard by using synchronous data combined with parallel factor analysis of the content of Amaranth.
- Published
- 2018
26. Double-balloon enteroscopy in small bowel diseases: Eight years single-center experience in China
- Author
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Guang-Wu Chen, Guo-Qiang Xu, Hua-Tuo Zhu, Ming Yang, Guodong Shan, Qing Gu, Min Yue, Wenguo Chen, Hong Zhang, Lihua Chen, and Lin L
- Subjects
Adult ,Male ,medicine.medical_specialty ,China ,Time Factors ,Adolescent ,diagnosis ,medicine.medical_treatment ,Perforation (oil well) ,capsule endoscopy ,Observational Study ,Aspiration pneumonia ,Single Center ,Gastroenterology ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Double-balloon enteroscopy ,Internal medicine ,Intestine, Small ,medicine ,double-balloon enteroscopy ,Humans ,Child ,Aged ,Retrospective Studies ,small bowel disease ,Aged, 80 and over ,medicine.diagnostic_test ,GiST ,business.industry ,Reproducibility of Results ,Retrospective cohort study ,General Medicine ,Middle Aged ,medicine.disease ,endoscopic findings ,Polypectomy ,Intestinal Diseases ,030220 oncology & carcinogenesis ,Acute pancreatitis ,030211 gastroenterology & hepatology ,Female ,business ,Follow-Up Studies ,Research Article - Abstract
The aim of the study was to evaluate the diagnostic and therapeutic value of double-balloon entoroscopy (DBE) in small bowel diseases (SBDs) in China. A retrospective review of 674 consecutive patients who underwent DBE between January 2007 and November 2015 was conducted. Patients were divided into 3 groups by age, young group (65 years). Data were collected with regard to demographics, clinical, endoscopic findings, complications, diagnostic yield, and management. A total of 729 DBE procedures were performed successfully in our series. More than 20 types of SBDs were found with the detection rate of 70.9%(517/729). The majority of patients were Crohn's disease (33.4%,225/674), followed by tumor (18.8%,127/674) and angioectasia (7.9%, 53/674). Endoscopic treatment was performed in 60 patients in which hemostasis (17,28.3%) and polypectomy (15,25%) were the predominant form of intervention used. Adverse events occurred in 6 patients (0.96%,6/729) including perforation, hemorrhage, aspiration pneumonia. No acute pancreatitis or other major complications occurred. Adenocarcinoma, GIST, and lymphoma were the most common tumor detected, the majority of tumors located in the jejunum (56.7%), The detection rate of angioectasia was also higher in the jejunum (54.7%),77.8% of Crohn's disease was located in the ileum. The positive rate of DBE in small bowel tumor and Crohn's disease were significantly higher than that of angioectasia (P 0.05). The obscure gastrointestinal bleeding (OGIB) was the most common indication, and the diagnostic yield was 71.8%. DBE is a useful diagnostic and therapeutic tool with high clinical practice value for the investigation of SBDs. With growing experience of endoscopist, we believe that DBE must be kept in mind as the first-line modality for suspected SBDs.
- Published
- 2016
27. [Not Available]
- Author
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Jin-Zhou, Zhu, Chun-Xiao, Li, Yi-Ning, Dai, De-Jian, Zhao, Zhi-Yun, Fang, Xing-Yong, Wan, Hua-Tuo, Zhu, Yu-Ming, Wang, Chao-Hui, Yu, and You-Ming, Li
- Subjects
Adult ,Male ,Metabolic Syndrome ,Angiopoietin-like Proteins ,Adipokines ,Anthropometry ,Angiopoietin-Like Protein 8 ,Case-Control Studies ,Peptide Hormones ,Humans ,Female ,Middle Aged - Abstract
la betatrofina es una novedosa adipoquina que provoca la proliferación de células β pancreáticas e interviene en el metabolismo de los lípidos. Objetivos: el propósito de este estudio es evaluar el papel de la betatrofina en el síndrome metabólico.se llevó a cabo un estudio hospitalario de casos y controles según sexo y edad. El nivel de betatrofina en suero fue evaluado mediante ensayo por inmunoabsorción ligado a enzimas. Se midieron las concentraciones en suero de 12 adipoquinas para evaluar las asociaciones con la betatrofina usando los kits comerciales Adipokine Magnetic Bead Panel. Los análisis estadísticos incluyeron correlación bivariada, análisis de curva ROC y análisis de regresión lineal multivariable.el nivel de betatrofina en suero fue más elevado en pacientes con síndrome metabólico (997,36 ± 475,92 pg/ml, p = 0,001) que en los controles (735,35 ± 526,51 pg/ml). Frente al tercil más bajo, el tercil más alto del nivel de betatrofina mostró una asociación con mayor riesgo de síndrome metabólico (odds ratio ajustado = 3,521, intervalo de confianza [IC] 95% [1,191-10,413], p = 0,023). Se desarrolló la curva ROC de betatrofina para pronosticar la presencia de síndrome metabólico (área bajo la curva ROC = 0,682 [95% IC, 0,597-0,767], p0,001). Además, la betatrofina mostró correlación con distintos parámetros, como edad (r = 0,286, p0,001), índice de masa corporal (r = 0,160, p = 0,046), índice cintura-cadera (r = 0,241, p = 0,002), lipoproteína de alta densidad (r = -0,167, p = 0,037), lipoproteína de baja densidad (r = -0,195, p = 0,015), glucosa plasmática en ayunas (r = 0,266, p = 0,001), hemoglobina A1C (r = 0,314, p0,001), índice de resistencia a la insulina mediante HOMA (r = 0,272, p = 0,001) y diversas adipoquinas, entre ellas resistina (r = 0,571, p0,001), interleucina-8 (r = 0,435, p0,001), factor de necrosis tumoral alfa (r = 0,295, p = 0,011) y lipocalina-2 (r = 0,346, p = 0,003).este estudio demuestra que la betatrofina en suero desempeña una importante labor en el síndrome metabólico, implicando la regulación del metabolismo de la glucosa y los lípidos y la inflamación.
- Published
- 2016
28. Serum betatrophin level increased in subjects with metabolic syndrome: a case-control study
- Author
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Jin-Zhou, Zhu, Chun-Xiao, Li, Yi-Ning, Dai, De-Jian, Zhao, Zhi-Yun, Fang, Xing-Yong, Wan, Hua-Tuo, Zhu, Yu-Ming, Wang, Chao-Hui, Yu, and You-Ming, Li
- Subjects
Síndrome metabólico ,Betatrophin ,Hepatokine ,Adipokine ,Hepatoquina ,Angiopoeitin-like 8 (ANGPTL8) ,Angiopoyetina-8 (ANGPTL8) ,Adipoquina ,Metabolic syndrome ,Betatrofina - Abstract
Background: Betatrophin is a novel adipokine that provokes pancreatic β-cell proliferation and is involved in lipid metabolism. Aims: This study aims to evaluate the role of serum betatrophin in metabolic syndrome (MetS). Methods: A hospital-based, age-/gender-matched case control study was conducted. The serum betatrophin level was evaluated by enzyme-linked immunosorbent assay. Serum concentrations of 12 adipokines were measured to assess their associations with serum betatrophin, using commercial Adipokine Magnetic Bead Panel kits. Statistical analyses included bivariate correlation, receiver operating characteristic (ROC) curve, and multivariate stepwise linear regression. Results: Serum betatrophin showed a higher level in MetS patients (997.36 ± 475.92 pg/ml, p = 0.001) compared with controls (735.35 ± 526.51 pg/ml). Compared with the lowest tertile, the highest tertile of serum betatrophin level indicated an association with higher risk of MetS (adjusted odds ratio = 3.521, 95% confidence interval [CI] [1.191-10.413], p = 0.023). ROC curve of betatrophin was developed to predict the presence of MetS (area under ROC = 0.682 [95% CI, 0.597-0.767], p < 0.001). Furthermore, betatrophin correlated with several parameters, e.g. age (r = 0.286, p < 0.001), body mass index (r = 0.160, p = 0.046), waist-to-hip ratio (r = 0.241, p = 0.002), high-density lipoprotein cholesterol (r = -0.167, p = 0.037), low-density lipoprotein cholesterol (r = -0.195, p = 0.015), fasting plasma glucose (r = 0.266, p = 0.001), hemoglobin A1C (r = 0.314, p < 0.001), homeostasis model assessment of insulin resistance (r = 0.272, p = 0.001), and various adipokines, e.g. resistin (r = 0. 571, p < 0.001), interleukin-8 (r = 0.435, p < 0.001), tumor necrosis factor-α (r = 0.295, p = 0.011) and lipocalin-2 (r = 0.346, p = 0.003). Conclusions: This study supports that serum betatrophin plays an important role in MetS, involving the regulations of glucose and lipid metabolism and inflammation. Introducción: la betatrofina es una novedosa adipoquina que provoca la proliferación de células β pancreáticas e interviene en el metabolismo de los lípidos. Objetivos: el propósito de este estudio es evaluar el papel de la betatrofina en el síndrome metabólico. Método: se llevó a cabo un estudio hospitalario de casos y controles según sexo y edad. El nivel de betatrofina en suero fue evaluado mediante ensayo por inmunoabsorción ligado a enzimas. Se midieron las concentraciones en suero de 12 adipoquinas para evaluar las asociaciones con la betatrofina usando los kits comerciales Adipokine Magnetic Bead Panel. Los análisis estadísticos incluyeron correlación bivariada, análisis de curva ROC y análisis de regresión lineal multivariable. Resultados: el nivel de betatrofina en suero fue más elevado en pacientes con síndrome metabólico (997,36 ± 475,92 pg/ml, p = 0,001) que en los controles (735,35 ± 526,51 pg/ml). Frente al tercil más bajo, el tercil más alto del nivel de betatrofina mostró una asociación con mayor riesgo de síndrome metabólico (odds ratio ajustado = 3,521, intervalo de confianza [IC] 95% [1,191-10,413], p = 0,023). Se desarrolló la curva ROC de betatrofina para pronosticar la presencia de síndrome metabólico (área bajo la curva ROC = 0,682 [95% IC, 0,597-0,767], p < 0,001). Además, la betatrofina mostró correlación con distintos parámetros, como edad (r = 0,286, p < 0,001), índice de masa corporal (r = 0,160, p = 0,046), índice cintura-cadera (r = 0,241, p = 0,002), lipoproteína de alta densidad (r = -0,167, p = 0,037), lipoproteína de baja densidad (r = -0,195, p = 0,015), glucosa plasmática en ayunas (r = 0,266, p = 0,001), hemoglobina A1C (r = 0,314, p < 0,001), índice de resistencia a la insulina mediante HOMA (r = 0,272, p = 0,001) y diversas adipoquinas, entre ellas resistina (r = 0,571, p < 0,001), interleucina-8 (r = 0,435, p < 0,001), factor de necrosis tumoral alfa (r = 0,295, p = 0,011) y lipocalina-2 (r = 0,346, p = 0,003). Conclusiones: este estudio demuestra que la betatrofina en suero desempeña una importante labor en el síndrome metabólico, implicando la regulación del metabolismo de la glucosa y los lípidos y la inflamación.
- Published
- 2016
29. Aumento del nivel de betatrofina en suero en sujetos con síndrome metabólico: estudio de casos y controles
- Author
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De-Jian Zhao, Xingyong Wan, Yi-Ning Dai, Chaohui Yu, Hua-Tuo Zhu, Youming Li, Yuming Wang, Chunxiao Li, Jinzhou Zhu, and Zhi-Yun Fang
- Subjects
Gynecology ,medicine.medical_specialty ,Nutrition and Dietetics ,Endocrinology ,Angiopoietin-like Protein ,Betatrophin ,business.industry ,Internal medicine ,medicine ,Medicine (miscellaneous) ,Metabolic syndrome ,medicine.disease ,business - Abstract
Introduccion: la betatrofina es una novedosa adipoquina que provoca la proliferacion de celulas β pancreaticas e interviene en el metabolismo de los lipidos. Objetivos: el proposito de este estudio es evaluar el papel de la betatrofina en el sindrome metabolico. Metodo: se llevo a cabo un estudio hospitalario de casos y controles segun sexo y edad. El nivel de betatrofina en suero fue evaluado mediante ensayo por inmunoabsorcion ligado a enzimas. Se midieron las concentraciones en suero de 12 adipoquinas para evaluar las asociaciones con la betatrofina usando los kits comerciales Adipokine Magnetic Bead Panel. Los analisis estadisticos incluyeron correlacion bivariada, analisis de curva ROC y analisis de regresion lineal multivariable. Resultados: el nivel de betatrofina en suero fue mas elevado en pacientes con sindrome metabolico (997,36 ± 475,92 pg/ml, p = 0,001) que en los controles (735,35 ± 526,51 pg/ml). Frente al tercil mas bajo, el tercil mas alto del nivel de betatrofina mostro una asociacion con mayor riesgo de sindrome metabolico (odds ratio ajustado = 3,521, intervalo de confianza [IC] 95% [1,191-10,413], p = 0,023). Se desarrollo la curva ROC de betatrofina para pronosticar la presencia de sindrome metabolico (area bajo la curva ROC = 0,682 [95% IC, 0,597-0,767], p < 0,001). Ademas, la betatrofina mostro correlacion con distintos parametros, como edad (r = 0,286, p < 0,001), indice de masa corporal (r = 0,160, p = 0,046), indice cintura-cadera (r = 0,241, p = 0,002), lipoproteina de alta densidad (r = -0,167, p = 0,037), lipoproteina de baja densidad (r = -0,195, p = 0,015), glucosa plasmatica en ayunas (r = 0,266, p = 0,001), hemoglobina A1C (r = 0,314, p < 0,001), indice de resistencia a la insulina mediante HOMA (r = 0,272, p = 0,001) y diversas adipoquinas, entre ellas resistina (r = 0,571, p < 0,001), interleucina-8 (r = 0,435, p < 0,001), factor de necrosis tumoral alfa (r = 0,295, p = 0,011) y lipocalina-2 (r = 0,346, p = 0,003). Conclusiones: este estudio demuestra que la betatrofina en suero desempena una importante labor en el sindrome metabolico, implicando la regulacion del metabolismo de la glucosa y los lipidos y la inflamacion.
- Published
- 2016
30. Influence of Oblique Incidence on Transmission Grating Diffraction in Soft X-Ray Region
- Author
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Sanwei Li, Wanli Shang, Wenhai Zhang, Jiamin Yang, Longyu Kuang, and Tuo Zhu
- Subjects
Diffraction ,Soft x ray ,Optics ,Materials science ,business.industry ,General Materials Science ,Oblique incidence ,business ,Diffraction grating - Published
- 2012
31. [Year Discrimination of Mild Aroma Chinese Liquors Using Three-Dimensional Fluorescence Spectroscopy Combined with Parallel Factor and Neural Network]
- Author
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Zhuo-wei, Zhu, Li-zhi, Que, Guo-qing, Chen, Rui-yu, Xu, and Tuo, Zhu
- Subjects
Spectrometry, Fluorescence ,Alcoholic Beverages ,Odorants ,Neural Networks, Computer - Abstract
Three-dimensional fluorescence spectroscopy coupled with parallel factor analysis and neural network was applied to the year discrimination of mild aroma Chinese liquors. The excitation-emission fluorescence matrices (EEMs) of 120 samples with various years were measured by FLS920 fluorescence spectrometer. The trilinear decomposition of the data array was performed and the loading scores of and the excitation-emission profiles of four components were also obtained. The scores were employed as the inputs of the BP neural networks and the PARAFAC-BP identification model was constructed. 10 samples were collected from 10, 20 and 30 years of liquors respectively, and 30 samples were selected as the test sets. The remaining 90 samples were used as the training sets to build the training model. The year prediction of unknown samples was also carried out, and the prediction accuracy was 90%, 100% and 100%, respectively. Meanwhile, the discrimination analysis method and the multi-way partial least squares discriminant analysis were compared, namely PARAFAC-BP and NPLS-DA. The results indicated that parallel factor combined with the neural network (PARAFAC-BP) has higher prediction accuracy. The proposed method can effectively extract the spectral characteristics, and also reduce the dimension of the input variables of neural network. A good year discrimination result was finally achieved.
- Published
- 2015
32. Experimental and Numerical Investigation on Electromagnetic Absorption and Diffraction by the Plasma-Covered Cavity
- Author
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Xiaojun Zeng, Ran Chu, Jianping Chen, Yudong Chen, Xiaowu Ni, Xiang He, and Tuo Zhu
- Subjects
Diffraction ,Physics ,Nuclear and High Energy Physics ,business.industry ,Scattering ,Plasma ,Condensed Matter Physics ,Electromagnetic radiation ,Optics ,Return loss ,Electromagnetic electron wave ,Transient (oscillation) ,Absorption (electromagnetic radiation) ,business - Abstract
To produce a plasma layer (area of 1.3 m2 and power of 1.8 kW) covering the inner surface of a metal cavity (diameter of 45 cm and length of 120 cm), a certain number of standard commercial fluorescent lamps were placed directly against one another. Electromagnetic (EM) scattering of a 1- to 3-GHz EM wave has been studied experimentally and theoretically for a metal cavity and a plasma-covered metal cavity. When the EM wave hits the cavity at the front aspect angle, a significant return loss of as large as 10 dB in the frequency of 1-2 GHz and trends to a peak of 20-30 dB near 1.25-1.5 GHz were observed. Using the finite-difference time-domain method, transient scattered fields of EM wave from cavities were achieved. The loss of the EM wave with covered plasma was proved to be a result of the absorption by the plasma, but not from the turning or bending of the wave entering the plasma.
- Published
- 2010
33. X-ray polarization measurement of the Beamline 4B7 of Beijing synchrotron radiation facility using a PET crystal
- Author
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LJ Sun, Tuo Zhu, Jiamin Yang, Jiyan Zhang, Fen Yan, Xin-Shi Gan, Yidong Zhao, Lei Zheng, Minxi Wei, Zhimin Hu, Rongqing Yi, Ming-Qi Cui, and Yang Zhao
- Subjects
Physics ,Nuclear and High Energy Physics ,Range (particle radiation) ,business.industry ,X-ray ,Synchrotron radiation ,Photon energy ,Polarization (waves) ,Crystal ,Optics ,Beamline ,business ,Instrumentation ,Beam (structure) - Abstract
An experimental method has been developed to measure the X-ray polarization of the Beamline 4B7 on Beijing Synchrotron Radiation Facility in the X-ray energy range 2100-5600 eV by using a PET crystal diagnostic system and rotating the system around the incident X-ray beam by 90 degrees. The experimental results show that the measured X-ray polarization is more than 0.88 in the photon energy range 2100-3600 eV with maximum of 0.99 at 2707.3 eV, and quickly decreases to about 0.38 at 4719.2 eV with photon energy increasing from 3600 to 4719.2 eV, then it keeps almost constant as the photon energy increases further to 5600 eV. (C) 2010 Elsevier B.V. All rights reserved.
- Published
- 2010
34. The investigation on hydrogenation platform for silicon solar cells based on high intensity infrared LEDs
- Author
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Li Chao, Guoqing Chen, Xi Xi, Rulong Chen, Jianbo Shao, Tuo Zhu, Wang Xiao, Guilin Liu, Weiying Qian, Guohua Li, and Yiqing Zhu
- Subjects
010302 applied physics ,Materials science ,Silicon ,Passivation ,Renewable Energy, Sustainability and the Environment ,Infrared ,business.industry ,chemistry.chemical_element ,02 engineering and technology ,Carrier lifetime ,021001 nanoscience & nanotechnology ,01 natural sciences ,law.invention ,Monocrystalline silicon ,Light intensity ,chemistry ,law ,0103 physical sciences ,Optoelectronics ,0210 nano-technology ,business ,Common emitter ,Light-emitting diode - Abstract
The research on the hydrogen passivation process for silicon solar cells has been developed for a long time. Recently, researchers have investigated the use of lasers as light sources. It has been found that hydrogen passivation technology can improve the minority carrier lifetime and passivate some impurities and defects in silicon solar cells. In this paper, a hydrogenation platform for silicon solar cells based on high intensity infrared LEDs of 940 nm was built. After a number of comparative experiments, electrical characteristics, photo-luminescence images, and light beam induced current were applied before and after hydrogenation to distinguish the passivation effect. It was found that the effect of hydrogen passivation on monocrystalline boron-doped passivated emitter and rear contact (PERC) solar cells by using infrared LEDs as the light source was satisfactory under the condition of suitable light intensity and temperature. It has a remarkable effect on improving the electrical characteristics an...
- Published
- 2018
35. Efficient soft x-ray sources from laser-irradiated gold foam targets with well-controlled impurities
- Author
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Tuo Zhu, Huabing Du, Xiayu Zhan, Tianming Song, Jiamin Yang, Yunsong Dong, Wanli Shang, Lu Zhang, Yongkun Ding, Wenhai Zhang, Feng Wang, Zhichao Li, Chengwu Huang, Shaoen Jiang, and Shenye Liu
- Subjects
Nuclear and High Energy Physics ,Brightness ,Materials science ,business.industry ,Astrophysics::High Energy Astrophysical Phenomena ,Energy conversion efficiency ,Nanotechnology ,Plasma ,Condensed Matter Physics ,Kinetic energy ,Laser ,01 natural sciences ,010305 fluids & plasmas ,law.invention ,law ,Impurity ,0103 physical sciences ,Optoelectronics ,Irradiation ,010306 general physics ,business ,Power density - Abstract
As an important x-ray source, enhancement of x-ray emissions from laser-produced plasmas is significant for various applications. Due to less expanding kinetic loss, gold foam with low initial density can have an enhanced x-ray conversion efficiency compared with solid-density gold. However, low-Z impurities within gold foam targets will diminish the enhancement remarkably, and should be tightly controlled. This paper presents an experimental study of a high brightness laser plasma soft x-ray source, based on a g gold foam target with negligible impurities irradiated by nanosecond laser pulses with power density around 3 × 10 W at the Shenguang II laser facility. A conversion efficiency, from multi-eV to multi-keV, of % is achieved in the x-ray emissions—about 21% relative enhancement compared with a solid-density gold target, and the highest conversion efficiency for Au foam planar targets yet. Good agreement has been achieved between the semi-analytical model prediction and the experimental results.
- Published
- 2017
36. Genome-wide Analysis of PTB-RNA Interactions Reveals a Strategy Used by the General Splicing Repressor to Modulate Exon Inclusion or Skipping
- Author
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Tongbin Wu, Hui Sun, Yuanchao Xue, Young-Soo Kwon, Xiong Ji, Tuo Zhu, Yi Zhang, Douglas L. Black, Chao Zhang, Yu Zhou, Gene W. Yeo, and Xiang-Dong Fu
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Molecular Sequence Data ,Exonic splicing enhancer ,macromolecular substances ,Biology ,environment and public health ,Exon ,Animals ,Humans ,Polypyrimidine tract-binding protein ,Molecular Biology ,Genetics ,Binding Sites ,integumentary system ,Genome, Human ,Gene Expression Profiling ,Alternative splicing ,Exons ,Cell Biology ,Exon skipping ,Alternative Splicing ,Polypyrimidine tract ,RNA splicing ,biology.protein ,RNA ,Minigene ,HeLa Cells ,Polypyrimidine Tract-Binding Protein ,Protein Binding - Abstract
Recent transcriptome analysis indicates that > 90% of human genes undergo alternative splicing, underscoring the contribution of differential RNA processing to diverse proteomes in higher eukaryotic cells. The polypyrimidine tract-binding protein PTB is a well-characterized splicing repressor, but PTB knockdown causes both exon inclusion and skipping. Genome-wide mapping of PTB-RNA interactions and construction of a functional RNA map now reveal that dominant PTB binding near a competing constitutive splice site generally induces exon inclusion, whereas prevalent binding close to an alternative site often causes exon skipping. This positional effect was further demonstrated by disrupting or creating a PTB-binding site on minigene constructs and testing their responses to PTB knockdown or overexpression. These findings suggest a mechanism for PTB to modulate splice site competition to produce opposite functional consequences, which may be generally applicable to RNA-binding splicing factors to positively or negatively regulate alternative splicing in mammalian cells.
- Published
- 2009
- Full Text
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37. Enhanced Biosensing Performance of Mesoporous SnO2 Multilayer Film in Interfacing Hemoglobin
- Author
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Jiu-Ju Feng, Jing-Juan Xu, Hong-Yuan Chen, and Jing-Tuo Zhu
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Materials science ,Biomedical Engineering ,Analytical chemistry ,Bioengineering ,General Chemistry ,Condensed Matter Physics ,Electrocatalyst ,Redox ,Dielectric spectroscopy ,Electron transfer ,chemistry.chemical_compound ,Adsorption ,chemistry ,General Materials Science ,Ferricyanide ,Ferrocyanide ,Mesoporous material - Abstract
Through alternative adsorption of dextran sulfate (DS) and mesoporous SnO2 (Meso-SnO2), {Meso-SnO2/DS}n films were constructed and applied for the immobilization of hemoglobin (Hb). For comparison, the same-sized non-mesoporous SnO2 nanopraticles (Nonmeso-SnO2) based films were also prepared. Thus-formed films were characterized by many techniques in detail. The electrochemical impedance spectroscopy (EIS, using ferricyanide/ferrocyanide as a redox probe) experiments confirmed the charge transfer resistance increased linearly with the number of Meso-SnO2/DS bilayers, which was smaller than that of Nonmeso-SnO2/DS bilayers. Further EIS, UV-vis and AFM experiments indicate that Meso-SnO2/DS film exhibit enhanced immobilization ability of Hb with favorable orientation in comparison with that of Nonmeso-SnO2/DS film. The adsorbed Hb at Meso-SnO2/DS film showed a good direct electron transfer behavior with the heterogeneous electron transfer rate constant (ks) of 8.89 +/-0.32 s(-1), and excellent electrocatalysis to the reduction of O2 and H2O2 in pH 7.0 buffers.
- Published
- 2009
38. Synthesis, characterization and photoluminescence of poly(VK-(ppy)2Ir(N-phMA))
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Yuqiang Ding, Guoqing Chen, Tuo Zhu, Weijie Wang, Hongbo Di, Cheng Li, and Chunxia Ren
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chemistry.chemical_classification ,Photoluminescence ,Ligand ,Mechanical Engineering ,Metals and Alloys ,chemistry.chemical_element ,Polymer ,Condensed Matter Physics ,Sodium methoxide ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,chemistry ,Mechanics of Materials ,Polymer chemistry ,Materials Chemistry ,Methacrylamide ,Iridium ,Luminescence ,Dichloromethane - Abstract
Reaction of N -phenyl methacrylamide ( N -phMA) with (ppy) 2 Ir(μ-Cl) 2 Ir(ppy) 2 (ppy = 2-phenylpyridine) in dichloromethane at the present of sodium methoxide at room temperature yields Iridium(III)-containing complex (ppy) 2 Ir( N -phMA) in which a N -substitued acrylamide was firstly applied as ancillary ligand. Complex (ppy) 2 Ir( N -phMA) can readily copolymerized with the commonly used N -vinyl carbazole (VK) for PLED materials in different feed ratios to generate a series of metal-containing polymers poly(VK-(ppy) 2 Ir( N -phMA))s whose chemical structures are well-defined. Investigations of thermal and photophysical properties indicate that these polymers are of interest materials for PLED.
- Published
- 2008
39. Propagation of Helmholtz-Gauss beams in weak turbulent atmosphere
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Yixin Zhang and Tuo Zhu
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Physics ,Field (physics) ,business.industry ,Irradiance ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,symbols.namesake ,Optics ,Phase perturbation ,Helmholtz free energy ,symbols ,Physics::Accelerator Physics ,Light beam ,Electrical and Electronic Engineering ,business ,Atmospheric optics ,Beam (structure) ,Gaussian beam - Abstract
Based on the Rytov approximation of light propagation in weak turbulent atmosphere, the closed-form expressions of field and average irradiance of each one of the four fundamental families of Helmholtz-Gauss (HzG) beams: cosine-Gauss beams, stationary Mathieu-Gauss beams, stationary parabolic-Gauss beams, and Bessel-Gauss beams, which are propagating in weak turbulent atmosphere, are obtained. The results show that the field and average irradiance can be written as the product of four factors: complex amplitude depending on the z-coordinate only, a Gaussian beam, a factor of complex phase perturbation induced by atmospheric turbulence, and a complex scaled version of the transverse shape of the non-diffracting beam. The effect of weak atmospheric turbulence on irradiance distribution of the HzG beam can be ignored.
- Published
- 2008
40. Direct electrochemistry and electrocatalysis of hemoglobin on undoped nanocrystalline diamond modified glassy carbon electrode
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Jing-Juan Xu, Hong-Yuan Chen, Chuan-Guo Shi, and Jing-Tuo Zhu
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Materials science ,Light ,Absorption spectroscopy ,Ultraviolet Rays ,Biophysics ,Analytical chemistry ,Nanoparticle ,engineering.material ,Electrocatalyst ,Electrochemistry ,Catalysis ,Electron Transport ,Hemoglobins ,Electron transfer ,Electric Impedance ,Physical and Theoretical Chemistry ,Electrodes ,Chitosan ,Spectrum Analysis ,Diamond ,Hydrogen Peroxide ,General Medicine ,Dielectric spectroscopy ,Electrode ,engineering ,Nanoparticles ,Glass - Abstract
Direct electrochemistry of hemoglobin (Hb) was observed at glassy carbon electrode (GCE) modified with undoped nanocrystalline diamond (UND) and Hb multilayer films via layer-by-layer assembly. UV-VIS absorbance spectroscopy, electrochemical impedance spectroscopy and cyclic voltammograms were employed to characterize the film. The results showed that the UND had the effect of enhancing the electron transfer between Hb and the electrode surface. Hb in the multilayer films maintained its bioactivity and structure. It also exhibited a good catalytic activity towards the reduction of H(2)O(2). The reciprocal of catalytic current showed a linear dependence on the reciprocal of H(2)O(2) concentration ranging from 0.5 microM to 0.25 mM with a detection limit of 0.4 microM. The apparent Michaelis-Menten constant was estimated to be 0.019 mM.
- Published
- 2007
41. Computational Prediction and Validation of BAHD1 as a Novel Molecule for Ulcerative Colitis
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Wenguo Chen, Hua-Tuo Zhu, Lu Han, Zhe Shen, Xiaochen Bo, Chao Lu, Jing Li, Lihua Chen, Xingyong Wan, Guo-Qiang Xu, Chaohui Yu, and Chenfu Xu
- Subjects
Small interfering RNA ,Chromosomal Proteins, Non-Histone ,Gene Expression ,Biology ,Models, Biological ,Inflammatory bowel disease ,Article ,Cell Line ,Gene Knockout Techniques ,Mice ,Gene expression ,medicine ,Animals ,Humans ,Computer Simulation ,Genetic Predisposition to Disease ,RNA, Small Interfering ,Gene ,Multidisciplinary ,Tumor Necrosis Factor-alpha ,JNK Mitogen-Activated Protein Kinases ,NF-kappa B ,Reproducibility of Results ,medicine.disease ,Transcription Factor AP-1 ,Disease Models, Animal ,Real-time polymerase chain reaction ,Receptors, Tumor Necrosis Factor, Type I ,Immunology ,Cancer research ,Colitis, Ulcerative ,I-kappa B Proteins ,Tumor necrosis factor alpha ,Inflammation Mediators ,Signal transduction ,Signal Transduction - Abstract
Ulcerative colitis (UC) is a common inflammatory bowel disease (IBD) producingintestinal inflammation and tissue damage. The precise aetiology of UC remainsunknown. In this study, we applied a rank-based expression profile comparativealgorithm, gene set enrichment analysis (GSEA), to evaluate the expression profilesof UC patients and small interfering RNA (siRNA)-perturbed cells to predict proteinsthat might be essential in UC from publicly available expression profiles. We usedquantitative PCR (qPCR) to characterize the expression levels of those genespredicted to be the most important for UC in dextran sodium sulphate (DSS)-inducedcolitic mice. We found that bromo-adjacent homology domain (BAHD1), a novelheterochromatinization factor in vertebrates, was the most downregulated gene. Wefurther validated a potential role of BAHD1 as a regulatory factor for inflammationthrough the TNF signalling pathway in vitro. Our findings indicate thatcomputational approaches leveraging public gene expression data can be used to inferpotential genes or proteins for diseases and BAHD1 might act as an indispensablefactor in regulating the cellular inflammatory response in UC.
- Published
- 2015
- Full Text
- View/download PDF
42. Serum periostin is a potential biomarker for non-alcoholic fatty liver disease: a case-control study
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Youming Li, Xingyong Wan, Fang Wang, Jinzhou Zhu, Zhi-Yun Fang, Yi-Ning Dai, Chaohui Yu, Chunxiao Li, De-Jian Zhao, Hua-Tuo Zhu, and Yuming Wang
- Subjects
0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,Pathology ,Endocrinology, Diabetes and Metabolism ,Adipokine ,Periostin ,Gastroenterology ,Sensitivity and Specificity ,03 medical and health sciences ,Endocrinology ,Non-alcoholic Fatty Liver Disease ,Internal medicine ,medicine ,Humans ,Aged ,Adiponectin ,business.industry ,Fatty liver ,Case-control study ,Middle Aged ,medicine.disease ,030104 developmental biology ,ROC Curve ,Case-Control Studies ,Biomarker (medicine) ,Resistin ,Female ,Steatohepatitis ,business ,Cell Adhesion Molecules ,Biomarkers - Abstract
Recent animal studies support close associations of Periostin with hepatosteatosis and steatohepatitis. This study is to evaluate the role of serum periostin in non-alcoholic fatty liver disease (NAFLD). A hospital-based age-/sex-matched case–control study was conducted. Binary logistic regression and receiver operating characteristic (ROC) curve were performed. Serum adipokines were measured by Adipokine Magnetic Bead Panel kits. The serum concentration of Periostin in NAFLD (1914.16 [1323.59–2654.88] ng/ml, P
- Published
- 2015
43. [Quantum Chemistry Calculation of Ponceau 4R Molecule Structure and Research on the Fluorescence Mechanism]
- Author
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Chun, Zhu, Yong, Zhang, Jian-chun, Wu, Tuo, Zhu, and Guo-qing, Chen
- Abstract
The molecule structures of Ponceau 4R in ground state and the excited state were optimized by employing the Gaussian 09W program package. In addition, the electronic structure and frontier orbital of the ground state, the emission wavelength of the excited state was also investigated. And then, the Edinburgh FLS920P fluorescence spectrometer was applied to the measurement of the fluorescence spectra of cochineal solution, and the emission spectra was obtained. The calculated emission wavelength had a good coincidence with the experiment data, which indicates that the optimized structures mentioned above are reasonable. The structures comparison between the ground state and the excited state was also performed to analyze the mechanism of fluorescence spectrum. It can be concluded that the molecule structure of excited state is nearly planar, so Ponceau 4R is thought to have strong fluorescent characteristics, the emission fluorescence is the result of transition from orbit 139 to orbit 137.
- Published
- 2015
44. A case-control study: Association between serum neuregulin 4 level and non-alcoholic fatty liver disease
- Author
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Youming Li, Chaohui Yu, Xingyong Wan, Hua-Tuo Zhu, Jinzhou Zhu, Zhi-Yun Fang, Yi-Ning Dai, and De-Jian Zhao
- Subjects
Adult ,Blood Glucose ,Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Body Mass Index ,chemistry.chemical_compound ,Endocrinology ,High-density lipoprotein ,Adipose Tissue, Brown ,Interquartile range ,Non-alcoholic Fatty Liver Disease ,Internal medicine ,medicine ,Humans ,Obesity ,Gamma-glutamyltransferase ,Risk factor ,education ,Aged ,Neuregulins ,Neuregulin-4 ,education.field_of_study ,biology ,Fatty liver ,Case-control study ,nutritional and metabolic diseases ,Middle Aged ,medicine.disease ,digestive system diseases ,Logistic Models ,chemistry ,Case-Control Studies ,biology.protein ,Female ,Body mass index - Abstract
Background Non-alcoholic fatty liver disease (NAFLD) is a great health burden. Neuregulin 4 (Nrg4) is a recently identified secret factor that may be associated with NAFLD. Aim To investigate the association between serum Nrg4 level and NAFLD by conducting a case–control study. Method A total of 174 subjects were included. 87 NAFLD subjects and 87 age- and sex-matched non-NAFLD controls were identified by hepatic ultrasound examination. Anthropometric and biochemical data were measured and recorded. Serum Nrg4 level was evaluated by using enzyme-linked immunosorbent assay. SPSS software was used for statistical analyses. Results Compared to the controls, subjects with NAFLD presented with reduced level of serum Nrg4 (0.40 (0.27, 0.55) vs. 0.50 (0.30, 0.81) ng/mL (median (interquartile range)), P = 0.029). By multivariate logistic regression analysis, reduced serum levels of Nrg4 were associated with higher NAFLD odds (OR = 0.251, 95% confidence interval = 0.081–0.779, P = 0.017). By dividing the distribution of serum Nrg4 level into quartiles, there was borderline statistical difference of NAFLD prevalence among the four groups ( P = 0.058). There was no significant difference of serum Nrg4 levels in subjects according to the grades of fatty liver by ultrasound ( P = 0.080). No statistical difference of serum Nrg4 level was observed between obese and non-obese subjects ( P = 0.932). Conclusion Decreased serum Nrg4 level is prevalent in NAFLD subjects compared to non-NAFLD controls, and is an independent risk factor associated with NAFLD, indicating that Nrg4 might have a protective role in the development of NAFLD.
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- 2015
45. A spontaneous hematoma arising within an intrapancreatic accessory spleen
- Author
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Feng-Ling Hu, Wenguo Chen, Lihua Chen, Qi-Qi Gao, Jinghua Yu, Guo-Qiang Xu, Guodong Shan, and Hua-Tuo Zhu
- Subjects
Adult ,Male ,accessory spleen ,Pathology ,medicine.medical_specialty ,medicine.medical_treatment ,Spleen ,Accessory spleen ,Diagnosis, Differential ,03 medical and health sciences ,Pancreatectomy ,0302 clinical medicine ,Hematoma ,medicine ,Humans ,Clinical Case Report ,Pancreas ,Pathological ,Abdomen, Acute ,business.industry ,intrapancreatic ,Pancreatic Diseases ,General Medicine ,medicine.disease ,respiratory tract diseases ,Pancreatic Neoplasms ,Treatment Outcome ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Abdomen ,030211 gastroenterology & hepatology ,Differential diagnosis ,business ,Research Article ,CT ,MRI - Abstract
Rational: Hematoma arising within an intrapancreatic accessory spleen (IPAS) is an extremely rare pathological entity. Patient concern: We present the case of a 39-year-old man with acute abdominal pain. Diagnoses: The patient was initially diagnosed as pancreatic cystic neoplasm according to CT and MRI imaging. Interventions: Distal pancreatectomy was conducted because of the possibility of malignancy. Outcomes: Surgical resection showed that the lesion was a hematoma in an IPAS. Lessons: Our case indicated that the differential diagnosis of hematoma in IPAS should be born in mind for cases with cystic neoplasm in tail of pancreas and an epidermoid cyst arising within an intrapancreatic accessory spleen (ECIAS).
- Published
- 2017
46. Molecular and structural characterization of New Red and Erythrosine by fluorescence polarization spectroscopy
- Author
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Jia-meng Du, Jin-chen Zhao, Tuo Zhu, Chun Zhu, and Guoqing Chen
- Subjects
0209 industrial biotechnology ,Materials science ,business.industry ,Statistical and Nonlinear Physics ,02 engineering and technology ,Condensed Matter Physics ,Erythrosine ,01 natural sciences ,Fluorescence ,Molecular physics ,chemistry.chemical_compound ,Dipole ,020901 industrial engineering & automation ,Optics ,chemistry ,Excited state ,0103 physical sciences ,010306 general physics ,business ,Anisotropy ,Ground state ,Spectroscopy ,Absorption (electromagnetic radiation) - Abstract
The fundamental and the fluorescence anisotropies of New Red and Erythrosine were measured. The intersection angles between the absorption and the emission dipole moments for New Red and Erythrosine are 4.44[Formula: see text] and 23.26[Formula: see text], respectively. The average angle shift of the emission dipole moment of New Red is 3.91[Formula: see text] during the lifetime of the excited state. This indicates that it has a bifurcated linear structure with weak rotational capacity. The average angle shift of the emission dipole moment of Erythrosine is 9.25[Formula: see text], indicating that it has a partial planar structure and is easier to rotate. The spatial ground state structures were simulated with Gaussian 09.
- Published
- 2017
47. Measurement of residual carbon in chamber of Shenguang II laser facility
- Author
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Tuo Zhu, Ruizhen Yu, S. Y. Liu, Fang Wang, Wan-Li Shang (尚万里), Shuqing Jiang, Jianlun Yang, and Wenhai Zhang
- Subjects
Physics ,business.industry ,Condensation ,Analytical chemistry ,Plasma ,Condensed Matter Physics ,Laser ,Residual ,01 natural sciences ,010305 fluids & plasmas ,law.invention ,Optics ,K-edge ,law ,Torr ,0103 physical sciences ,010306 general physics ,business ,Inertial confinement fusion ,FOIL method - Abstract
It is reported that in National Ignition Facility's shock timing campaign, there is a 2 μm layer of residual air condensation on the cold laser entrance hole window, and residual gas level of ∼5×10−6 Torr ( ∼6.6×10−4 Pa) in the chamber [Robey et al., Phys. Plasmas 19, 042706 (2012)]. On Shenguang (SG) II and III laser facilities, a nominal residual gas level in the chamber is ∼10−2 Pa in experiment. In this work, the residual Carbon (C) level in the SG II chamber is investigated experimentally using a transmission grating spectrometer (TGS). The experimental result shows that there is a clear C K edge (280–300 eV), which implies that there is residual C condensation on the charge-coupled device (CCD) silicon oxide layer. The effect of the C condensation on the CCD response is studied with a condensed-C-foil model. With the modified CCD responses, the unfolded spectra by TGS are shown, and the significant C K edge decreases as the C foil thickness increases. Compared to the simulated C K edge depth, the me...
- Published
- 2017
48. Radiative cooling induced plasma collapse observed in laser irradiation of a CH-tamped gold micro-disk
- Author
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Bo Deng, Yang Zhao, Xiayu Zhan, Bo Qing, Zheng Yuan, Min Lv, Gang Xiong, Yongkun Ding, Tuo Zhu, Jiamin Yang, Jiyan Zhang, Zhimin Hu, and Rongqing Yi
- Subjects
Physics ,Physics and Astronomy (miscellaneous) ,Radiative cooling ,business.industry ,Collapse (topology) ,Plasma ,Laser ,01 natural sciences ,010305 fluids & plasmas ,law.invention ,Transverse plane ,Optics ,Cooling rate ,Physics::Plasma Physics ,law ,0103 physical sciences ,Irradiation ,Laser pulse duration ,Atomic physics ,010306 general physics ,business - Abstract
Time-resolved x-ray self-emission imaging was used to study the dynamic evolution of a laser-produced gold plasma tamped by plastic (CH), and a significant plasma collapse was observed during the laser irradiation. The plasma collapse, a kind of transverse contraction, has been ascribed to the radial compression caused by the different radiative cooling rates and thus different pressures between the central high-Z gold plasma and the surrounding low-Z CH plasma, and this has been reproduced by numerical simulations using the two-dimensional radiation-hydrodynamics code Multi2D. The experimental results represent an observation of the radiative cooling induced plasma jet within a 1 ns laser pulse duration, much more quickly than those reported previously. In addition, our experiment design may offer a method to study the radiative cooling rates of high-Z plasmas. The measured cooling rate is a factor of 2 higher than the theoretical result [Post et al., At. Data Nucl. Data Tables 20, 397 (1977)], but is wi...
- Published
- 2017
49. Determination of components of mixed color solutions by fluorescence spectroscopy combined with calibration algorithms
- Author
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Chun, Zhu, Run, Li, Li-Zhi, Que, Tuo, Zhu, and Guo-Qing, Chen
- Subjects
Spectrometry, Fluorescence ,Calibration ,Food Coloring Agents ,Factor Analysis, Statistical ,Algorithms - Abstract
The three-dimensional spectra of mixed solutions of allure red, sunset yellow and brilliant blue were obtained. Then the three synthetic food colors were determined by parallel factor analysis (PARAFAC) and alternating trilinear decomposition (ATLD) algorithms, respectively. The component number of model is three by core-consistency diagnostic. The average recoveries of allure red, sunset yellow and brilliant blue obtained by PARAFAC were 98.75% +/- 8.9%, 97.22% +/- 2.9% and 99.00% +/- 2.9% and those by ATLD algorithm were 99.78% +/- 5.9%, 92.52% +/- 5.5% and 97.23% +/- 5.8%, respectively. Results show that both of the algorithms can be used in direct and rapid determination of multi-components of mixtures. From further comparison, the PARAFAC is more stable and advantageous.
- Published
- 2014
50. Is Helicobacter pylori infection associated with glycemic control in diabetics?
- Author
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Chaohui Yu, Wei-lai Yu, Yi-Ning Dai, Hua-Tuo Zhu, Jiexia Ding, and Youming Li
- Subjects
Adult ,Blood Glucose ,Male ,medicine.medical_specialty ,Adolescent ,macromolecular substances ,Cochrane Library ,Helicobacter Infections ,Risk Factors ,Diabetes mellitus ,Internal medicine ,medicine ,Odds Ratio ,Humans ,Hypoglycemic Agents ,Child ,Glycemic ,Glycated Hemoglobin ,Chi-Square Distribution ,biology ,Helicobacter pylori ,business.industry ,Gastroenterology ,Proton Pump Inhibitors ,General Medicine ,Odds ratio ,Middle Aged ,medicine.disease ,biology.organism_classification ,bacterial infections and mycoses ,Confidence interval ,Anti-Bacterial Agents ,Endocrinology ,Diabetes Mellitus, Type 1 ,Treatment Outcome ,Diabetes Mellitus, Type 2 ,Meta-analysis ,Drug Therapy, Combination ,Female ,business ,Chi-squared distribution ,Biomarkers ,Meta-Analysis - Abstract
AIM: To investigate whether Helicobacter pylori (H. pylori) infection is associated with glycemic control and whether hyperglycemia is modified by eradication therapy. METHODS: The databases of PubMed, Cochrane Library, Chinese BioMedicine Web Base and Chinese Science and Technology Journals were searched from inception to June 2014. Studies examining the association between H. pylori infection and glycemic control and⁄or the effect of eradication treatment on glycemic control in diabetic humans were eligible for inclusion. Meta-analyses were conducted using the Review Manager software version 5.2. The outcome measures are presented as weighed mean differences (WMDs) with 95% confidence intervals (CIs). Statistical heterogeneity was assessed by the Cochran Q test and the I2 statistic. RESULTS: A total of 21 relevant publications were identified. A meta-analysis of 11 studies with 513 patients with diabetes mellitus (DM) showed significantly lower glycosylated hemoglobin (HbA1c) levels in the H. pylori-negative than H. pylori-positive DM participants (WMD = 0.43, 95%CI: 0.07-0.79; P = 0.02). In children and adolescents with type 1 DM (T1DM), there was a positive association between H. pylori infection and HbA1c level (WMD = 0.35, 95%CI: 0.05-0.64; P = 0.02), but there was no difference in those with type 2 DM (T2DM, WMD = 0.51, 95%CI: -0.63-1.65; P = 0.38). A meta-analysis of six studies with 325 T2DM participants showed a significant difference in the fasting plasma glucose levels between H. pylori-positive and H. pylori-negative participants (WMD = 1.20, 95%CI: 0.17-2.23; P = 0.02). Eradication of H. pylori did not improve glycemic control in the T2DM participants in a three-month follow-up period (HbA1c decrease: WMD = -0.03, 95%CI = -0.14-0.08; P = 0.57; fasting plasma glucose decrease: WMD = -0.06, 95%CI: -0.36-0.23; P = 0.68). Glycemic control was significantly better in T1DM participants who were not reinfected than in those who were reinfected (HbA1c: WMD = 0.72, 95%CI: 0.32-1.13: P = 0.00). CONCLUSION: H. pylori infection is associated with poorer glycemic control in T1DM patients, but eradication may not improve glycemic control in DM in a short-term follow-up period.
- Published
- 2014
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