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Background Host-derived miRNAs are reported to play diverse roles in dengue virus (DENV) infection, but DENV-derived miRNAs have been rarely studied. Methods Here, we used serum samples from three patients infected with dengue virus type 1 (DENV1) and three healthy volunteers to detect and profile novel microRNAs in dengue virus-infected human serum. MicroRNAs in serum samples were sequenced using an Illumina HiSeq 2000 system, and clean reads were trimmed, aligned, normalized, and analyzed to identify differentially expressed and novel microRNAs. Four microRNAs were selected as DENV1 infection biomarkers and verified through 1:2 paired case-control study, using serum from 15 DENV1-infected patients and 30 healthy volunteers. Results We identified 182 potential novel miRNAs, with 20 novel miRNAs found to have miRDeep2 score ≥ 4.0. Fifty-eight known and 11 novel miRNAs were upregulated at least 2-fold in DENV1-infected serum. Twenty-two known and 4 novel miRNAs were downregulated at least 0.5-fold. The AUCs of four selected miRNAs, hsa-miR-106b-3p, hsa-miR-122-5p, hsa-miR-novel-chr17_35150, and hsa-miR-novel-chr10_24390, were respectively: 0.962 (95% CI: 0.913 - 1.000, p > 0.05), 0.924 (95% CI: 0.851 - 0.998, p 0.05), and 0.991 (95% CI: 0.973 - 1.000, p > 0.05). Conclusions In summary, our study demonstrates that serum miRNA levels are affected by DENV1 infection, identifies novel DENV1-associated miRNAs, and suggests that Hsa-miR-122-5p may be a potential biomarker for DENV1 infection.

Higher vocational colleges carry out entrepreneurship and innovation education activities to serve the important subjects of Rural Revitalization Strategy. At present, there are deficiencies in innovation and entrepreneurship education in higher vocational colleges, which must be treated seriously, so as to explore the effective path to promote the effective integration of the two, not only to provide intellectual support for the smooth realization of Rural Revitalization Strategy, but also to provide college students with a good platform for employment and entrepreneurship

With the strong support of the state, the innovation and entrepreneurship education in Colleges and universities has achieved remarkable results. In the new era, high-quality innovation and entrepreneurship education is needed to truly achieve innovation leading. At present, there are some problems in the innovation and entrepreneurship education, such as inadequate understanding of innovation and entrepreneurship education, unreasonable curriculum system, insufficient teachers and poor practical platform. This paper mainly analyzes the problems in the innovation and entrepreneurship education in colleges and universities from the above aspects and puts forward the corresponding measures and countermeasures.

In the new era, the state has given high expectations for vocational education. The Central Committee of the Communist Party of China, with Comrade Xi Jinping as its core, has placed vocational education in a very prominent strategic position, and has put forward a series of scientific decisions and major decisions and plans. Xi Jinping pointed out for the first time in the nineteen major reports of the Communist Party of China that implementing the strategy of Rural Revitalization is a major move to win the goal of building a well-off society in an all-round way. The integration and development of innovation education of college students and entrepreneurship practice of “Rural Revitalization” is not only conducive to the cultivation of innovation and entrepreneurship ability of college students, but also can help solve the problems of rural areas, agriculture and farmers, and effectively promote the harmonious and stable development of rural economy. In this paper, from the research background of College Students’ innovation and entrepreneurship and rural revitalization, this paper will analyze how vocational colleges should serve rural revitalization.