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2 results on '"Tamara P. Hamilton"'

1. Synthesis of new fluorogenic cyanine dyes and incorporation into RNA fluoromodules

Author

Kelly L. Robertson, Kaitlin Fague, Tudor P. Constantin, Tamara P. Hamilton, Bruce A. Armitage, and Alan S. Waggoner, and Gloria L. Silva

Subjects
Chemistry, Aptamer, Organic Chemistry, Analytical chemistry, RNA, Quantum yield, Fluorine, Carbocyanines, Biochemistry, Combinatorial chemistry, Fluorescence, chemistry.chemical_compound, Fluid solution, Spectrometry, Fluorescence, Glycerol, Physical and Theoretical Chemistry, Cyanine, DNA
Abstract

A new fluorogenic cyanine dye was synthesized and found to have low fluorescence quantum yield in fluid solution and in the presence of double-stranded DNA but 80-fold enhanced fluorescence in viscous glycerol solution. An RNA aptamer selected for binding to the new dye exhibits K(d) = 87 nM and 60-fold fluorescence enhancement. The dye-aptamer pair is a fluoromodule that can be incorporated into fluorescent sensors and labels.

Published
2008

2. The spectrum of Trp- mutants isolated as 5-fluoroanthranilate-resistant clones in Saccharomyces bayanus, S. mikatae and S. paradoxus

Author

Sean Conroy, Elizabeth W. Jones, Fatmata Barrie, Bur Chu, Alexander I. Greenwood, Jonathan Stahlman, Tamara P. Hamilton, Samantha Conroy, Peter B. Berget, Diane Garrity, Ruby Chen, Blake Sweeney, Seda Avetisian, James M. Burnette, Simon Hucko, Tiffany Monaco, Carmen Jackson, Elizabeth Wiltrout, Sharon Shung, Candice Anderson, Samantha Spath, William A. Eimer, Allyson Dill, Andrea Zonneveld, George Schaeffer, Daniel Yurovsky, Christina Onorato, Mai Otsuka, Kristen Livesey, Satyan Pai, and Denise Asafu-Adjei

Subjects
Genetics, Homothallism, Fungal protein, biology, Mutant, Genetic Complementation Test, Saccharomyces bayanus, Indole-3-Glycerol-Phosphate Synthase, Tryptophan, Bioengineering, biology.organism_classification, Applied Microbiology and Biotechnology, Biochemistry, Paradoxus, Saccharomyces, Complementation, Fungal Proteins, Mutation, ortho-Aminobenzoates, Ploidy, Aldose-Ketose Isomerases, Biotechnology, Anthranilate Synthase
Abstract

5-Fluoroanthranilic acid (FAA)-resistant mutants were selected in homothallic diploids of three Saccharomyces species, taking care to isolate mutants of independent origin. Mutations were assigned to complementation groups by interspecific complementation with S. cerevisiae tester strains. In all three species, trp3, trp4 and trp5 mutants were recovered. trp1 mutants were also recovered if the selection was imposed on a haploid strain. Thus, FAA selection may be more generally applicable than was previously described.

Published
2007

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