1. The effect of C-type natriuretic peptide on delayed rectifier potassium currents in gastric antral circular myocytes of the guinea-pig
- Author
-
Zhang Y, Xu Hy, Huang X, Yang M, Piao Lh, Sun Jb, Gao L, and Xu Wx
- Subjects
Male ,medicine.medical_specialty ,Patch-Clamp Techniques ,Physiology ,Potassium ,Muscle Relaxation ,Guinea Pigs ,Myocytes, Smooth Muscle ,chemistry.chemical_element ,Motility ,Second Messenger Systems ,Membrane Potentials ,Guinea pig ,chemistry.chemical_compound ,Internal medicine ,medicine ,Pyloric Antrum ,Myocyte ,Animals ,Phosphodiesterase inhibitor ,Protein kinase A ,Cyclic GMP ,Cells, Cultured ,Natriuretic Peptide, C-Type ,General Medicine ,Endocrinology ,chemistry ,Collagenase ,Female ,Zaprinast ,Gastrointestinal Motility ,medicine.drug ,Delayed Rectifier Potassium Channels ,Signal Transduction - Abstract
C-type natriuretic peptides (CNP) play an inhibitory role in smooth muscle motility of the gastrointestinal tract, but the effect of CNP on delayed rectifier potassium currents is still unclear. This study was designed to investigate the effect of CNP on delayed rectifier potassium currents and its mechanism by using conventional whole-cell patch-clamp technique in guinea-pig gastric myocytes isolated by collagenase. CNP significantly inhibited delayed rectifier potassium currents [I(K (V))] in dose-dependent manner, and CNP inhibited the peak current elicited by depolarized step pulse to 86.1+/-1.6 % (n=7, P
- Published
- 2007