1. Successive Release of Tissue Inhibitors of Metalloproteinase-1 Through Graphene Oxide-Based Delivery System Can Promote Skin Regeneration
- Author
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Yixiong Zheng, Cheng Zhong, Peter J. Nelson, Qi Bao, and Dike Shi
- Subjects
Materials science ,Biocompatibility ,Context (language use) ,Nanotechnology ,02 engineering and technology ,03 medical and health sciences ,0302 clinical medicine ,TIMP-1 ,In vivo ,lcsh:TA401-492 ,medicine ,General Materials Science ,Fibroblast ,Graphene oxide ,Skin repair ,Successive release ,Regeneration (biology) ,Nano Idea ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Skin regeneration ,medicine.anatomical_structure ,Apoptosis ,030220 oncology & carcinogenesis ,Biophysics ,lcsh:Materials of engineering and construction. Mechanics of materials ,0210 nano-technology ,Wound healing - Abstract
The purpose of this study was to testify the hypothesis that graphene oxide (GO) could act as an appropriate vehicle for the release of tissue inhibitors of metalloproteinase-1 (TIMP-1) protein in the context of skin repair. GO characteristics were observed by scanning electron microscopy, atomic force microscopy, and thermal gravimetric analysis. After TIMP-1 absorbing GO, the release profiles of various concentrations of TIMP-1 from GO were compared. GO biocompatibility with fibroblast viability was assessed by measuring cell cycle and apoptosis. In vivo wound healing assays were used to determine the effect of TIMP-1-GO on skin regeneration. The greatest intensity of GO was 1140 nm, and the most intensity volume was 10,674.1 nm (nanometer). TIMP-1 was shown to be continuously released for at least 40 days from GO. The proliferation and viability of rat fibroblasts cultured with TIMP-1-GO were not significantly different as compared with the cells grown in GO or TIMP-1 alone (p > 0.05). Skin defect of rats treated with TIMP-1 and TIMP-1-GO showed significant differences in histological and immunohistochemical scores (p
- Published
- 2017