1. Prevalence, risk factors and molecular characterization of Cryptosporidium infection in cattle in Addis Ababa and its environs, Ethiopia
- Author
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Mulatu Tesfaye, Mamo Gezahegn, Tilahun Getachew, Wegayehu Teklu, Anberber Manyazewal, Stomeo Francesca, Muthui Lucy, and Mahendra Pal
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0301 basic medicine ,Veterinary medicine ,Cryptosporidium infection ,animal diseases ,Cryptosporidium ,Article ,PCR-RFLP ,03 medical and health sciences ,parasitic diseases ,Prevalence ,medicine ,Weaning ,Parasite hosting ,Dairy cattle ,General Veterinary ,biology ,030108 mycology & parasitology ,medicine.disease ,biology.organism_classification ,Cryptosporidium parvum ,Risk factors ,Herd ,Parasitology ,Ethiopia ,Nested polymerase chain reaction - Abstract
A cross-sectional study was conducted to determine the prevalence and risk factors of Cryptosporidium infection and identify species of the parasite in cattle in central Ethiopia. Faecal samples, collected from 392 dairy cattle managed under intensive and extensive production system, were analyzed by the Modified Ziehl-Neelsen (MZN) microscopy, Nested PCR, PCR-RFLP and sequence analyses of the SSU rRNA gene of Cryptosporidium. The overall prevalence, the prevalence in the extensive and intensive farms was 18.6%, 11% and 21%, respectively. The infection was detected in 37.7% of the investigated farms with prevalence range of 7.4% -100%, and all of the six surveyed districts with significant (P = 0.000) prevalence difference. Restriction digestion and sequence analysis showed Cryptosporidium parvum and C. andersoni in 27% and 73% of the infections, respectively, showing an age related distribution pattern, C. parvum exclusively occurring in calves, Highlights • Cryptosporidium infection, due to C. parvum and C. andersoni, is widespread in cattle in central Ethiopia. • C parvum and C andersoni exclusively occurred in calves more...
- Published
- 2018
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