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Supplemental material, sj-docx-1-jdr-10.1177_00220345211069956 for Nociceptor Neurons Magnify Host Responses to Aggravate Periodontitis by S. Wang, X. Nie, Y. Siddiqui, X. Wang, V. Arora, X. Fan, V. Thumbigere-Math and M.K. Chung in Journal of Dental Research

Supplemental material, sj-docx-1-jdr-10.1177_00220345211069956 for Nociceptor Neurons Magnify Host Responses to Aggravate Periodontitis by S. Wang, X. Nie, Y. Siddiqui, X. Wang, V. Arora, X. Fan, V. Thumbigere-Math and M.K. Chung in Journal of Dental Research

Objective: A novel treatment for bucco-accessory root canal (bARC), which is difficult to treat by regular endodontic therapy, is discussed. Clinical Presentation and Intervention: A bARC in the maxillary right incisor with symptomatic irreversible pulpitis was detected using cone beam computed tomography (CBCT) after conventional dental radiography. The pulp was extirpated and the canal enlarged using rotary files and chemo-mechanical reagents. The main root canal and bARC were obturated with thermoplastic gutta-percha using continuous wave condensation. Postoperative CBCT revealed a completely treated bARC. Conclusion: A combination of CBCT and continuous wave condensation is ideal for bARCs in incisors.

H-FUNCTIONS AND LARGE DEFLECTION OF A CIRCULAR PLATE

PRH/HHEX (proline-rich homeodomain protein/haematopoietically expressed homeobox protein) is a transcription factor that controls cell proliferation, cell differentiation and cell migration. Our previous work has shown that in haematopoietic cells, Protein Kinase CK2-dependent phosphorylation of PRH results in the inhibition of PRH DNA-binding activity, increased cleavage of PRH by the proteasome and the misregulation of PRH target genes. Here we show that PRH and hyper-phosphorylated PRH are present in normal prostate epithelial cells, and that hyper-phosphorylated PRH levels are elevated in benign prostatic hyperplasia, prostatic adenocarcinoma, and prostate cancer cell lines. A reduction in PRH protein levels increases the motility of normal prostate epithelial cells and conversely, PRH over-expression inhibits prostate cancer cell migration and blocks the ability of these cells to invade an extracellular matrix. We show that CK2 over-expression blocks the repression of prostate cancer cell migration and invasion by PRH. In addition, we show that PRH knockdown in normal immortalised prostate cells results in an increase in the population of cells capable of colony formation in Matrigel, as well as increased cell invasion and decreased E-cadherin expression. Inhibition of CK2 reduces PRH phosphorylation and reduces prostate cell proliferation but the effects of CK2 inhibition on cell proliferation are abrogated in PRH knockdown cells. These data suggest that the increased phosphorylation of PRH in prostate cancer cells increases both cell proliferation and tumour cell migration/invasion.

Bambara groundnut production is constrained by inherent low soil phosphorus (P) content and high cost of water-soluble P fertilizers. A screenhouse study on the root growth, N and P uptake of Bambara groundnut was conducted to evaluate the effect of rock phosphate application at The University of Nottingham Malaysia Campus. Treatments consisted of factorial combination of two Bambara groundnut landraces (Kangiwa brown and Kaaro) and four rock phosphate (0, 20, 40 and 60 kg P2O5 ha-1) levels. The results revealed that Kaaro landrace was higher than Kangiwa brown in root length, volume, surface area, length volume-1, root dry weight, plant dry weight and N and P content. Application of rock phosphate at 40 kg P2O5 ha-1 was optimum in root volume, surface area and root dry weight. However, plant dry weight as well as N, P and ash contents were optimum at 20 kg P2O5 ha-1. Thus, rock phosphate could serve as an alternative source of P for Bambara groundnut production in the study area. Application of 20 kg P2O5 ha-1 (0.28 g kg-1 of soil) was optimum for the N and P uptake of the crop. Overall Kaaro landrace performed better than Kangiwa brown in the study area.