1. GENERATION OF CD4-POSITIVE SUPPRESSOR T CELLS FROM MIXED LYMPHOCYTE CULTURES IN THE PRESENCE OF INTERLEUKIN 2 RECEPTOR ANTIBODY TÜ69
- Author
-
E. Schneider, Graham Pawelec, Peter Wernet, Shi Lr, and H J Bühring
- Subjects
Antigens, Differentiation, T-Lymphocyte ,Interleukin 2 ,T-Lymphocytes ,Lymphocyte ,Biology ,Lymphocyte Activation ,T-Lymphocytes, Regulatory ,Antigen ,Interleukin-4 receptor ,Immune Tolerance ,medicine ,Humans ,Receptors, Immunologic ,Interleukin 5 ,Interleukin 3 ,Transplantation ,Antibodies, Monoclonal ,Interleukin ,Receptors, Interleukin-2 ,Molecular biology ,Clone Cells ,Tolerance induction ,medicine.anatomical_structure ,Antigens, Surface ,Immunology ,Lymphocyte Culture Test, Mixed ,medicine.drug - Abstract
The specificity of a novel monoclonal antibody (moAB), TU69, directed to the interleukin 2 receptor (IL-2R) was verified by sequential immunoprecipitation with anti-Tac. TU69 cross-competed with anti-Tac in binding analyses. When TU69 was added during the sensitization of normal peripheral blood mononuclear cells (PBMC) to allogeneic HLA-class I or -class II mismatched stimulator PBMC, alloproliferative responses and specific cytotoxicity were no longer detectable and the generation of natural killer (NK)-like effector cells was partially inhibited. Remarkably, however, the generation of CD4+ nonspecific suppressor T cells in such mixed lymphocyte cultures (MLC) was not inhibited--but, in contrast, was strongly enhanced in the presence of TU69. These suppressor cells inhibited unrelated allospecific responses in vitro to background levels even at a ratio of 50:1 responder:irradiated suppressor T cell lines. Such a potent experimental suppressor system suggests a possible application of TU69 for in vivo tolerance induction after transplantation, by down-regulating allospecific effector cells and allowing the generation of tolerance to graft antigens.
- Published
- 1987