1. In Vitro Study of Leishmanicidal Activity of Biogenic Selenium Nanoparticles against Iranian Isolate of Sensitive and Glucantime-Resistant Leishmania tropica
- Author
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MAHMOUDVAND, Hossein, SHAKIBAIE, Mojtaba, TAVAKOLI, Razieh, JAHANBAKHSH, Sareh, and SHARIFI, Iraj
- Subjects
inorganic chemicals ,Medical Sciences ,Cutaneous leishmaniasis ,Glucantime resistant ,technology, industry, and agriculture ,Amastigote ,lcsh:RC109-216 ,Promastigote ,Selenium nanoparticles ,lcsh:Infectious and parasitic diseases - Abstract
Background: Sensitive and glucantime (MA) resistance Leishmania tropica are referred to those isolates, which are responsive, or non-responsive to one or two full courses of treatment by MA systematically and/or intra-lesionally, respectively. In this study, we evaluated the antileishmanial activity of biogenic selenium nanoparticles (Se NPs) alone and in combination with MA against sensitive and glucantimeresistant L. tropica on in vitro model. Methods: The Se NPs were synthesized by employing the Bacillus sp. MSh-1. The antileishmanial effects of Se NPs alone and in combination with MA on promastigote and amastigote stages of sensitive and glucantime-resistant L. tropica strains have been investigated using a colorimetric MTT assay and in a macrophage model. In addition hemolytic activity in type O+ human red blood cells and infectivity rate of the promastigotes before and after treatment with the Se NPs was evaluated. Results: In the promastigote stage, various concentrations of Se NPs significantly inhibited (P
- Published
- 2014