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28 results on '"Sedlak J"'

1. Experimentální stanovení transformačního diagramu kontinuálního chlazení pro vysokopevnostní ocel X155CrMoV12

Author

Krbaťa, M., Majerik, J., Barenyi, I., Eckert, M., Čep, R., Sedlak, J., and Samardžić, I.

Subjects
dilatometrie, Mining engineering. Metallurgy, X155CrMoV12, dilatometry, cooling rate, microstructure, CCT diagram, TN1-997, mikrostruktura, rychlost chlazení
Abstract

The article is a result of investigations which deals with the phase transformations of tool steel X155CrMoV12. The experimental data obtained was used to evaluate the resulting Continuous Cooling Transform (CCT) diagram, which consists of seven dilation curves. All experimental samples from dilatometric analyzes were then subjected to microstructural analyzes and hardness measurements to characterize the microstructure and hardness for each heat treatment mode tested. Atomic Force Microscopy (AFM) microscopy was also used to study the carbides present in steels and their size and shape for all selected cooling modes. Příspěvek je výsledkem výzkumu, který se zabývá fázovými přeměnami nástrojové oceli X155CrMoV12. Získaná experimentální data byla použita k vyhodnocení výsledného diagramu kontinuální chladicí transformace (CCT), který se skládá ze sedmi dilatačních křivek. Všechny experimentální vzorky z dilatometrických analýz byly následně podrobeny mikrostrukturním analýzám a měřením tvrdosti s cílem charakterizovat mikrostrukturu a tvrdost pro každý testovaný způsob tepelného zpracování. Mikroskopie atomárních sil (AFM) byla rovněž použita ke studiu karbidů přítomných v ocelích a jejich velikosti a tvaru pro všechny vybrané režimy chlazení.

Published
2022

2. Potential biomarkers of exosomal cargo in endocrine signaling

Author

Zduriencikova M, Sedlak J, D. Cholujova, and P. Gronesova

Subjects
Cell type, Survivin, Endocrinology, Diabetes and Metabolism, Biology, Exosomes, Inhibitor of Apoptosis Proteins, Paracrine signalling, Endocrinology, Breast cancer, Predictive Value of Tests, Neoplasms, Paracrine Communication, Biomarkers, Tumor, medicine, Animals, Humans, Autocrine signalling, Lung cancer, Stomach cancer, Cancer, Prognosis, medicine.disease, Microvesicles, Autocrine Communication, MicroRNAs, Immunology, Cancer research, Endocrine Cells, Signal Transduction
Abstract

Cancer represents one of the leading cause of morbidity and mortality worldwide, with approximately 14 million new cases every year and more than 8 million cancer related deaths. In men, lung cancer is the most frequently diagnosed cancer type, followed by the prostate, colorectal, and gastric cancer; in woman, the most frequent cancer is the breast cancer, followed by the colorectal, lung, cervical, and stomach cancer. During the second half of the twentieth century the efforts to evaluate the importance of the solid tumor cells present in the circulating blood have been made. Similarly, long time ago in 1948, extracellular nucleic acids (circulating free DNA) floating around in human blood plasma were discovered. Exosomes were disclosed as the last component of this "triumvirate" present in the blood and applicable for diagnostics. The exosomal cargo consists of bioactive molecules from donor cells that can be transferred to recipient cells and modulate their intracellular signaling. Thus, exosomes can provide autocrine (local signals between the same cell type), paracrine (local signals between different cell types), and endocrine (distant signals between any types of cells) type of signals.

Published
2015

4. Deciphering the genetic determinism of flowering/harvest period and several fruit sensory quality traits in apple by a Genome-Wide Association approach

Author

Urrestarazu, Jorge, Moriya, Shigeki, Muranty, Helene, Denance, Caroline, Leforestier, Diane, Ravon, Elisa, Guyader, Arnaud, Guisnel, Rémi, Feugey, Laurence, Tartarini, Stefano, Dondini, Luca, Grégori, R., Lateur, Marc, Houben, P., Sedlak, J., Paprstein, F., Ordidge, Matthew, Nybom, H., Garkava-Gustavsson, L., Troggio, Michela, Bianco, Luca, Velasco, R., Poncet, Charles, Theron, Anthony, Bink, Marco C. A. M., Laurens, Francois, Durel, Charles Eric, Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO), Centre Wallon de Recherches Agronomiques (CRA-W), Research and Breeding Institute of Pomology Holovousy (VSUO), University of Reading (UOR), Swedish University of Agricultural Sciences (SLU), Centro Ricerca e Innovazione, Fondazione Edmund Mach, Instituto Agrario S. Michele all' Adige, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Wageningen University and Research Centre (WUR), Institut National de Recherche Agronomique (INRA). UMR Institut de Recherche en Horticulture et Semences (1345)., and Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP)

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

5. In vitro shoot proliferation of sweet cherry cultivars Karešova and Rivan

Author

Sedlak, J., Vyzkumny a Slechtitelsky Ustav Ovocnarsky, Holovousy and Paprstein, F., Vyzkumny a Slechtitelsky Ustav Ovocnarsky, Holovousy

Subjects
0106 biological sciences, Plant propagation, 04 agricultural and veterinary sciences, Horticulture, Biology, 040401 food science, 01 natural sciences, In vitro, Tissue culture, 0404 agricultural biotechnology, Murashige and Skoog medium, Micropropagation, Thidiazuron, Shoot, Cultivar, 010606 plant biology & botany
Abstract

The objective of this study was to investigate the possibility of optimizing routine tissue culture methods to proliferate two sweet cherry cultivars Karesova and Rivan. Shoot tips of two genotypes were successfully established in vitro. Six proliferation MS media containing 1, 2 and 4 mg/l BAP (6-benzylaminopurine), 0.5 and 1 mg/l TDZ (thidiazuron) or 10 mg/l 2iP (6-(γ,γ-dimethylallylamino)purine) were tested. The highest proliferation rate (3.0 ± 0.1) was obtained for Rivan on MS medium containing 2 mg/l BAP. In the case of cultivar Karesova, any of the cytokinins tested did not promote satisfactory proliferation. The highest proliferation rate (1.6) achieved on MS medium with 2 mg/l 2iP is not sufficient for a larger scale in vitro shoot production. It was proved that different genotypes of sweet cherry do not respond in the same way during proliferation in vitro. Future research and testing of other media and plant growth regulators will be carried out.

Published
2008

6. Genome Wide Association Study of two phenology traits (flowering time and maturity date) in apple

Author

Muranty, Helene, Urrestarazu, Jorge, Denance, Caroline, Leforestier, Diane, RAVON, ELISA, Guyader, Arnaud, Guisnel, Rémi, Feugey, Laurence, Tartarini, S., Dondini, L., Grégori, R., Lateur, M., Houben, P., Sedlak, J., Paprstein, T., Ordidge, Matthew, Nybom, H., Garkava-Gustavsson, L., Troggio, M., Bianco, Luca, Velasco, R., Bink, M. C. A. M., Laurens, Francois, Durel, Charles Eric, Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO), Centre Wallon de Recherches Agronomiques (CRA-W), University of Reading (UOR), Swedish University of Agricultural Sciences (SLU), Centro Ricerca e Innovazione, Fondazione Edmund Mach, Instituto Agrario S. Michele all' Adige, Wageningen UR Biometris, Wageningen University and Research [Wageningen] (WUR), UE, European Project: 265582,EC:FP7:KBBE,FP7-KBBE-2010-4,FRUIT BREEDOMICS(2011), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

9. Resistance of human multidrug-resistant neoplastic cell lines to paclitaxel-induced-radiosensitization is reduced by the non-immunosuppressive cyclosporine analog SDZ PSC 833

Author

Sedlak J, Hunakova L, Chorvath M, Sulikova M, Novotny L, Boljesikova E, Robert Zeillinger, and Chorvath B

Subjects
Ovarian Neoplasms, Radiation-Sensitizing Agents, Paclitaxel, Cell Survival, Cell Cycle, Apoptosis, Cyclosporins, HL-60 Cells, DNA, Neoplasm, Antineoplastic Agents, Phytogenic, Combined Modality Therapy, Radiation Tolerance, Drug Resistance, Multiple, Antineoplastic Combined Chemotherapy Protocols, Tumor Cells, Cultured, Humans, Drug Interactions, Female, ATP Binding Cassette Transporter, Subfamily B, Member 1, Phospholipids
Abstract

The non-immunosuppressive cyclosporine analog SDZ PSC 833 abolished the resistance of human multidrug resistant (MDR-1, P-gp) human promyelocyte leukemia HL-60/VCR cells in vitro to paclitaxel-induced cell cycle- and viability alterations, as well as resistance to paclitaxel-induced radiosensitization. Furthermore, SDZ PSC 833 abolished also the resistance of human multidrug-resistant ovarian A2780/ADR cells to paclitaxel-induced cell cycle alterations and reduced its resistance to paclitaxel-induced radiosensitization. In these multidrug-resistant ovarian carcinoma cells the supra-additive interaction between paclitaxel and SDZ PSC 833 pre-exposure and subsequent irradiation appeared at slightly higher paclitaxel concentrations (40-100 nM) compared to those required for a similar interaction in the parental drug sensitive A2780 cells (40-80 nM paclitaxel).

Published
1998

10. Measurement of inclusive cross-sections for production of cumulative protons in pi-, K-, and anti-p interactions in Be at 40-GeV/c

Author

Antipov, Yu M., Batarin, V. A., Bezzubov, V. A., Bilenky, Mikhail S., Budanov, N. P., Vishnevsky, A. V., Gorin, Yu P., Yury Gornushkin, Denisov, D. S., Eroshin, O. V., Kartasheva, V. G., Kulinich, P. A., Mitselmakher, Guenakh, Mandrichenko, I. V., Nozdrin, A. A., Olszewski, A. G., Palombo, F., Petrukhin, A. I., Pirtskhalava, R. V., Polovnikov, S. A., Roinishvili, V. N., Sedlak, J., Stoyanova, D. A., and Frabetti, P. L.

11. Study of fast p, anti-p yields in pi- Be interaction at 40-GeV/c with cumulative particle production

Author

Antipov, Yu M., Batarin, V. A., Bezzubov, V. A., Bilenky, Mikhail S., Budanov, N. P., Vishnevsky, A. V., Gorin, Yu P., Yury Gornushkin, Denisov, D. S., Eroshin, O. V., Kartasheva, V. G., Kulinich, P. A., Leitner, R., Mitselmakher, Guenakh, Mandrichenko, I. V., Nozdrin, A. A., Olshevsky, A. G., Palombo, F., Petrukhin, A. I., Pirtzkhalava, R. V., Roinishvili, V. N., Sedlak, J., Stoyanova, D. A., and Frabetti, P. L.

12. SIGMA-AJAX SETUP FOR THE STUDY OF ELASTIC pi- p AND K- p SCATTERING

Author

Vishnevsky, A. V., Golutvin, I. A., Yury Gornushkin, Karzhavin, V. Yu, Kulinich, P. A., Leitner, R., Mitselmakher, Guenakh, Nozdrin, A. A., Olszewski, A. G., Popov, A. A., Riemann, S., Sedlak, J., Smolin, D. A., Toledo, R., Travkin, V. I., Khabarov, V. S., Antipov, Yu M., Batarin, V. A., Bessubov, V. A., Budanov, N. P., Gorin, Yu P., Denisov, D. S., Eroshin, O. V., Kartasheva, V. G., Kotov, I. V., Melnik, Yu M., Petrukhin, A. I., Polovnikov, S. A., Stoyanova, D. A., Pirtskhalava, R. V., and Roinishvili, V. N.

15. MEASUREMENT OF COUPLING CONSTANT GAMMA ---> 3 PI IN THE PROCESS OF PION PAIR PRODUCTION BY PIONS IN NUCLEAR COULOMB FIELD. (IN RUSSIAN)

Author

Antipov, Yu M., Batarin, V. A., Bessubov, V. A., Budanov, N. P., Gorin, Yu P., Yury Gornushkin, Denisov, S. P., Klimenko, S. V., Kotov, I. V., Kulinich, P. A., Lebedev, A. A., Mitselmakher, Guenakh, Olszewski, A. G., Palombo, F., Petrukhin, A. I., Pirtskhalava, R., Polovnikov, S. A., Roinishvili, V. N., Sedlak, J., Stoyanova, D. A., and Travkin, V. I.

16. ELASTIC SCATTERING OF pi- AND K- MESONS ON PROTONS AT 43-GeV/c MOMENTUM

Author

Antipov, Yu M., Batarin, V. A., Bezzubov, V. A., Budanov, N. P., Gorin, Yu P., Denisov, D. S., Eroshin, O. V., Kartasheva, V. G., Kotov, I. V., Melnik, Yu M., Petrukhin, A. I., Polovnikov, S. A., Stoyanova, D. A., Vishnevsky, A. V., Golutvin, I. A., Yury Gornushkin, Kulinich, P. A., Leitner, R., Mitselmakher, Guenakh, Nozdrin, A. A., Olszewski, A. G., Riemann, S., Sedlak, J., Pirtskhalava, R. V., and Roinishvili, V. N.

18. Search for dibaryon resonances in processes with cumulative protons emitted from pi- Be interactions at 40-GeV/c

Author

Antipov, Yu M., Batarin, V. A., Bezzubov, V. A., Bilenky, Mikhail S., Budanov, N. P., Vishnevsky, A. V., Gorin, Yu P., Gornushkin, Yu A., Denisov, D. S., Eroshin, O. V., Kartasheva, V. G., Kulinich, P. A., Leitner, R., Guenakh Mitselmakher, Mandrichenko, I. V., Nozdrin, A. A., Olshevsky, A. G., Palombo, F., Petrukhin, A. I., Pirtzkhalava, R. V., Polovnikov, S. A., Roinishvili, V. N., Sedlak, J., Stoyanova, D. A., and Frabetti, P. L.

19. Diffractional excitation of Be nucleus by 40-GeV/c pions accompanied by proton emissions into backward hemisphere

Author

Antipov, Yu M., Batarin, V. A., Bezzubov, V. A., Bilenky, Mikhail S., Vishnevsky, A. V., Gorin, Yu P., Gornushkin, Yu A., Denisov, D. S., Eroshin, O. V., Kartasheva, V. G., Kulinich, P. A., Leitner, R., Guenakh Mitselmakher, Mandrichenko, I. V., Nozdrin, A. A., Olshevsky, A. G., Palombo, F., Petrukhin, A. I., Roinishvili, V. N., Sedlak, J., Stoyanova, D. A., and Frabetti, P. L.

20. Estimation of the secondary particles emission range in the cumulative particle productions

Author

Antipov, Yu M., Batarin, V. A., Bessubov, V. A., Bilenky, Mikhail S., Budanov, N. P., Vishnevsky, A. V., Gorin, Yu P., Gornushkin, Yu A., Denisov, D. S., Eroshin, O. V., Kartasheva, V. G., Kulinich, P. A., Leitner, R., Guenakh Mitselmakher, Mandrichenko, I. V., Nozdrin, A. A., Olszewski, A. G., Palombo, F., Petrukhin, A. I., Pirtskhalava, R., Polovnikov, S. A., Roinishvili, V. N., Sedlak, J., Stoyanova, D. A., and Frabetti, P. L.

25. Inclusive cross-section of cumulative proton production in pi- (K-, anti-p) Be, Al, Cu, Pb interactions at 40-GeV/c

Author

Antipov, Yu M., Batarin, V. A., Bezzubov, V. A., Bilenky, Mikhail S., Budanov, N. P., Vishnevsky, A. V., Gorin, Yu P., Gornushkin, Yu A., Denisov, D. S., Eroshin, O. V., Kartasheva, V. G., Kulinich, P. A., Leitner, R., Guenakh Mitselmakher, Mandrichenko, I. V., Nozdrin, A. A., Olszewski, A. G., Palombo, F., Petrukhin, A. I., Pirtskhalava, R. V., Polovnikov, S. A., Roinishvili, V. N., Sedlak, J., Stoyanova, D. A., and Frabetti, P. L.

26. Spectrometer for cumulative particle detection in hadron - nuclear interaction at 40-GeV/c

Author

Antipov, Yu M., Batarin, V. A., Bezzubov, V. A., Bilenky, Mikhail S., Budanov, N. P., Vishnevsky, A. V., Gorin, Yu P., Yury Gornushkin, Denisov, D. S., Eroshin, O. V., Kartasheva, V. G., Kulinich, P. A., Leitner, R., Mitselmakher, Guenakh, Mandrichenko, I. V., Nozdrin, A. A., Olszewski, A. G., Palombo, F., Petrukhin, A. I., Pirtskhalava, R. V., Polovnikov, S. A., Roinishvili, V. N., Sedlak, J., Stoyanova, D. A., and Frabetti, P. L.

27. Combining genetic resources and elite material populations to improve the accuracy of genomic prediction in apple

Author

Xabi Cazenave, Bernard Petit, Marc Lateur, Hilde Nybom, Jiri Sedlak, Stefano Tartarini, François Laurens, Charles-Eric Durel, Hélène Muranty, Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Wallon de Recherches Agronomiques (CRA-W), University College of Kristianstad, Research and Breeding Institute of Pomology Holovousy (VSUO), University of Bologna, INRAE metaprogram SelGen and more specifically from the GdivSelgen project. The framework of the regional programme 'Objectif Végétal, Research, Education and Innovation in Pays de la Loire', supported by the French Region Pays de la Loire, Angers Loire Métropole and the European Regional Development Fund. The Commission of the European Communities (contract N° QLK5-CT-822 2002-01492), Directorate—General Research— Quality of Life and Management of Living Resources Programme., European Project: 265582,EC:FP7:KBBE,FP7-KBBE-2010-4,FRUIT BREEDOMICS(2011), European Project: 817970,H2020 INVITE, Université d'Angers (UA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), University of Bologna/Università di Bologna, Cazenave X., Petit B., Lateur M., Nybom H., Sedlak J., Tartarini S., Laurens F., Durel C.-E., and Muranty H.

Subjects
0106 biological sciences, Genotype, Malus domestica, 01 natural sciences, Polymorphism, Single Nucleotide, genomic selection, [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics, 03 medical and health sciences, training set design, population combination, Genetics, Selection, Genetic, Agricultural Science, Molecular Biology, Genetics (clinical), 030304 developmental biology, 0303 health sciences, Genome, Models, Genetic, Genomics, Shared Data Resource, germplasm, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, Plant Breeding, GenPred, Phenotype, Genomic Prediction, Malus, Genomic, 010606 plant biology & botany
Abstract

Genomic selection is an attractive strategy for apple breeding that could reduce the length of breeding cycles. A possible limitation to the practical implementation of this approach lies in the creation of a training set large and diverse enough to ensure accurate predictions. In this study, we investigated the potential of combining two available populations, i.e., genetic resources and elite material, in order to obtain a large training set with a high genetic diversity. We compared the predictive ability of genomic predictions within-population, across-population or when combining both populations, and tested a model accounting for population-specific marker effects in this last case. The obtained predictive abilities were moderate to high according to the studied trait and small increases in predictive ability could be obtained for some traits when the two populations were combined into a unique training set. We also investigated the potential of such a training set to predict hybrids resulting from crosses between the two populations, with a focus on the method to design the training set and the best proportion of each population to optimize predictions. The measured predictive abilities were very similar for all the proportions, except for the extreme cases where only one of the two populations was used in the training set, in which case predictive abilities could be lower than when using both populations. Using an optimization algorithm to choose the genotypes in the training set also led to higher predictive abilities than when the genotypes were chosen at random. Our results provide guidelines to initiate breeding programs that use genomic selection when the implementation of the training set is a limitation.

Published
2022

28. Genome wide association study of two phenology traits (flowering time and maturity date) in apple

Author

Diane Leforestier, Marco C. A. M. Bink, Laurence Feugey, Matthew Ordidge, Charles Poncet, Luca Bianco, Elisa Ravon, Jorge Urrestarazu, Frantisek Paprstein, Larisa Garkava-Gustavsson, Caroline Denancé, Helene Muranty, Charles-Eric Durel, Stefano Tartarini, Riccardo Velasco, Arnaud Guyader, Anthony Théron, M. Troggio, Luca Dondini, Marc Lateur, Jiri Sedlak, Roberto Gregori, François Laurens, Hilde Nybom, Rémi Guisnel, Patrick Houben, Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-AGROCAMPUS OUEST-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Fondazione Edmund Mach - Edmund Mach Foundation [Italie] (FEM), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), S. Tartarini, H. Nybom, F. Laurens, L. Dondini, Muranty, H., Urrestarazu, J., Denancã©, C., Leforestier, D., Ravon, E., Guyader, A., Guisnel, R., Feugey, L., Tartarini, Stefano, Dondini, Luca, Gregori, Roberto, Lateur, M., Houben, P., Sedlak, J., Paprstein, F., Ordidge, M., Nybom, H., Garkava-gustavsson, L., Troggio, M., Bianco, L., Velasco, R., Poncet, C., Thã©ron, A., Bink, M. C. A. M., Laurens, F., Durel, C. E., Université d'Angers (UA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Université d'Angers (UA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
0106 biological sciences, 0301 basic medicine, Germplasm, Linkage disequilibrium, [SDV]Life Sciences [q-bio], Single-nucleotide polymorphism, Genome-wide association study, Biology, Quantitative trait locus, Horticulture, 01 natural sciences, 03 medical and health sciences, Malus × domestica, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Germplasm collection, Malus x domestica, 2. Zero hunger, Genetics, Phenology, fungi, food and beverages, Marker-assisted selection, PE&RC, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, Biometris, 030104 developmental biology, Germplasm collections, Trait, germplasm collections, 010606 plant biology & botany
Abstract

International audience; The aim of Genome Wide Association Studies (GWAS) is to identify markers in tight linkage disequilibrium with loci controlling quantitative trait variation. These markers can then be used in marker-assisted selection (MAS) in fruit crops such as apple. The GWAS approach involves both phenotyping of a large population of mostly unrelated individuals for the traits of interest, and genotyping at high marker density. In the EU-FP7 project FruitBreedomics, almost 1,200 European diploid dessert apple accessions (old and/or local cultivars) from six germplasm collections were genotyped with the Affymetrix Axiom_Apple480K array (487,000 SNPs). Phenotypic data on a large number of traits have been gathered during the project. Here we focus on flowering period and harvesting date. Knowledge of the genetic control of these traits is necessary to develop cultivars that can face the challenges imposed by global climate change and to target cultivar development as a function of a prolonged vegetation period in the production regions. Different models were tested, including control for effects of population structure and relatedness between cultivars. The full model, controlling for both structure and relatedness, was shown to be the most appropriate to avoid spurious marker-trait associations. When analyzing data over all collections, one significant marker-trait association was obtained for each trait, on chromosomes 9 and 3, for flowering period and harvesting date, respectively. Thereby, genomic locations previously identified in bi-parental populations could now be confirmed for a genetically diverse germplasm.

Published
2017

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