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32 results on '"Samoggia, P"'

1. Coordinate expression and proliferative role of HOXB genes in activated adult T lymphocytes

Author

Alessandra Carè, Testa, U., Bassani, A., Tritarelli, E., Montesoro, E., Samoggia, P., Cianetti, L., and Peschle, C.

Subjects
Cell Biology, Molecular Biology
Abstract

We investigated the expression of HOXB cluster genes in purified phytohemagglutinin (PHA)-activated T lymphocytes from normal adult peripheral blood by reverse transcription PCR and RNase protection. These genes are not expressed in quiescent T cells, except for barely detectable B1 RNA. After the PHA stimulus, HOXB gene activation initiates coordinately as a rapid induction wave in the 3'-->5' cluster direction (i.e., from HOXB1 through B9 genes). Thus, (i) expression of the foremost 3'-located B1 and B2 genes peaks 10 min after PHA addition and then rapidly declines, (ii) activation of B3, B4, and B5 begins 10 min after PHA addition and peaks at later times (i.e., at 120 min for B5), (iii) B6, B7, and B9 are expressed at a low level starting at later times (45 to 60 min), and (iv) B8 remains silent. Treatment of PHA-activated T lymphocytes with antisense oligonucleotides to B2 or B4 mRNA causes a drastic inhibition of T-cell proliferation and a decreased expression of T-cell activation markers (i.e., interleukin 2 and transferrin receptors). Similarly, treatment of CEM-CCRF, Peer, and SEZ627 T acute lymphocytic leukemia cell lines with anti-B4 oligomer markedly inhibits cell proliferation. Finally, T cells stimulated by a low dosage of PHA in the presence of 1 microM retinoic acid show a marked increase of both HOXB expression, particularly B2, and cell proliferation. These studies provide novel evidence on the role of HOX genes in adult cell proliferation. (i) Coordinate, early activation of HOXB genes from the 3'-->5' cluster side apparently underlies T-cell activation. (ii) The expression pattern in adult PHA-activated T cells is strikingly similar to that observed in retinoic acid-induced teratocarcinoma cells (A. Simeone, D. Acampora, L. Arcioni, P. W. Andres, E. Boncinelli, and F. Mavilio, Nature (London) 346:763-766, 1990), thus suggesting that molecular mechanisms underlying HOX gene expression in the earliest stages of development may also operate in activated adult T lymphocytes.

Published
1994

6. [Potentials and limitations of viscometry in hemorheological studies]

Author

Salvo, G., Samoggia, P., Camagna, A., Bartolomei, M., Costa, A. M., Stegagno, Michele, and Martino, R.

Subjects
Erythrocyte Aggregation, Erythrocyte Deformability, Centrifugation, Density Gradient, Hemodynamics, Methods, Humans, Blood Viscosity, Rheology, Mathematics
Abstract

The problems of viscometric studies are discussed in depth on the basis of the most recent advances in hemorheology. We present here our contribution to the solution of numerous methodological problems. Particular emphasis was set on the problem of optimization and standardization of viscometric techniques for the study of erythrocyte deformation and aggregation.

Published
1985

13. IFN-beta selectively down-regulates transferrin receptor expression in human peripheral blood macrophages by a post-translational mechanism

Author

Testa U, Conti L, Nm, Sposi, Varano B, Tritarelli E, walter malorni, Samoggia P, Rainaldi G, Peschle C, and Belardelli F

Subjects
Adult, Male, Adolescent, Macrophages, Immunology, Transferrin, Down-Regulation, Interferon-beta, Blotting, Northern, Flow Cytometry, Monocytes, Iodine Radioisotopes, Interferon-gamma, Receptors, Transferrin, Immunology and Allergy, Humans, Female, Protein Processing, Post-Translational, Cells, Cultured
Abstract

We evaluated the effect of IFN-beta on the expression of transferrin receptor (TfR) during the in vitro differentiation of peripheral blood monocytes to macrophages. IFN-beta exerted a strong inhibitory effect on the expression of TfR. As little as 0.1 IU/ml was sufficient to induce a 40% reduction of transferrin (Tf) binding sites on 7-day cultured macrophages. Scatchard plot analysis revealed that this impaired Tf binding in IFN-beta-treated macrophages was not due to a decreased affinity of the TfR for its ligand but to a reduction in the number of cell surface TfR. IFN-gamma did not exert any significant effect on the expression of TfR, even though it was capable of partially reverting the inhibitory effect of the IFN-beta on Tf binding. To understand the mechanism by which IFN-beta inhibited TfR expression, we examined the expression of TfR mRNA, 125I-Tf binding to detergent-solubilized cells, and TfR cellular distribution. The results of these experiments showed that IFN-beta caused neither a significant alteration of the expression of TfR mRNA nor a decrease of the total content of TfR molecules. Moreover, immunofluorescence analysis of TfR localization indicated that TfR was clustered in an intracellular compartment in IFN-beta-treated macrophages. These data demonstrate that IFN-beta is capable of dramatically down-modulating TfR in macrophages by post-translational mechanisms (i.e., by sequestering this receptor in intracellular compartments).

27. Interleukin 2 in cancer therapy

Author

Testa, U., Montesoro, E., Bulgarini, D., Samoggia, P., Masciulli, R., Habetswallner, D., Alessandra Carè, Mariani, G., Giannella, G., Boccoli, G., Scalzo, S., Rocca, E., Sonsini, C., Salvo, G., Tritarelli, E., Cerio, A. M., Gengaro, A., Mastroberardino, G., and Camagna, A.

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