1. Energy efficient bead milling of microalgae: Effect of bead size on disintegration and release of proteins and carbohydrates
- Author
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K. Yonathan, René H. Wijffels, Maria J. Barbosa, E. Suarez-Garcia, Michel H.M. Eppink, Carl Safi, Giuseppe Olivieri, P.R. Postma, Postma, P. R, Suarez Garcia, E, Safi, C, Yonathan, K, Olivieri, Giuseppe, Barbosa, M. J, Wijffels, R. H, and Eppink, M. H. M.
- Subjects
0106 biological sciences ,Bio Process Engineering ,Environmental Engineering ,Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 [VDP] ,Chlorella vulgaris ,Bioengineering ,010501 environmental sciences ,Bead ,7. Clean energy ,01 natural sciences ,Bead milling ,Algae ,Chlorophyta ,010608 biotechnology ,Phase (matter) ,Microalgae ,Waste Management and Disposal ,Protein release ,0105 earth and related environmental sciences ,Hexoses ,VLAG ,Chromatography ,Energy ,biology ,Renewable Energy, Sustainability and the Environment ,Chemistry ,Stress parameters ,RuBisCO ,Algal Proteins ,Water ,General Medicine ,Specific energy consumption ,biology.organism_classification ,Neochloris oleoabundans ,Native Polyacrylamide Gel Electrophoresis ,Tetraselmis suecica ,Kinetics ,visual_art ,visual_art.visual_art_medium ,biology.protein ,BBP Biorefinery & Sustainable Value Chains ,Microscopy, Electrochemical, Scanning ,Bead size - Abstract
The disintegration of three industry relevant algae (Chlorella vulgaris, Neochloris oleoabundans and Tetraselmis suecica) was studied in a lab scale bead mill at different bead sizes (0.3–1 mm). Cell disintegration, proteins and carbohydrates released into the water phase followed a first order kinetics. The process is selective towards proteins over carbohydrates during early stages of milling. In general, smaller beads led to higher kinetic rates, with a minimum specific energy consumption of ⩽0.47 kWh kgDW−1 for 0.3 mm beads. After analysis of the stress parameters (stress number and stress intensity), it appears that optimal disintegration and energy usage for all strains occurs in the 0.3–0.4 mm range. During the course of bead milling, the native structure of the marker protein Rubisco was retained, confirming the mildness of the disruption process.
- Published
- 2017