1. Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector
- Author
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R Carretto, Harald Vaessin, Larry Ackerman, Ellsworth Grell, Kimberly McCall, S Shepherd, E Bier, S Barbel, K Lee, and Tadashi Uemura
- Subjects
Embryo, Nonmammalian ,Recombinant Fusion Proteins ,Genetic Vectors ,medicine.disease_cause ,Nervous System ,P element ,Drosophilidae ,Genetics ,medicine ,Animals ,Gene ,Regulation of gene expression ,Reporter gene ,Mutation ,biology ,beta-Galactosidase ,biology.organism_classification ,Biological Evolution ,Phenotype ,Drosophila melanogaster ,Gene Expression Regulation ,DNA Transposable Elements ,Genes, Lethal ,Developmental Biology - Abstract
A P-element vector has been constructed and used to generate lines of flies with single autosomal P-element insertions. The lines were analyzed in two ways: (1) the identification of cis-acting patterning information within the Drosophila genome, as revealed by a lacZ reporter gene within the P element, and (2) the isolation of lethal mutations. We examined 3768 independent lines for the expression of lacZ in embryos and looked among these lines for lethal mutations affecting embryonic neurogenesis. This type of screen appears to be an effective way to find new loci that may play a role in the development of the Drosophila nervous system.
- Published
- 1989
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