Search

Purpose: This study evaluated the impact of 1-day workshops on teachers’ knowledge, practices, and dispositions using known characteristics of quality professional development and Guskey’s five levels of professional development evaluation. Method: Eight workshops were evaluated over a 2-year period using pre/post surveys, end-of-workshop surveys, observations, interviews, and artifacts. Results: Participants reported high levels of satisfaction and trainer effectiveness scores at the end of workshops. Statistical analyses revealed improvements in four of six outcome variables 4 weeks after workshop completion: self-reported knowledge, utilization of implementation strategies, presence of a community of continued learning, and teacher efficacy. Qualitative data corroborated these results but offered mixed evidence of teacher implementation and improved student outcomes. Discussion/Conclusion: Findings confirm that 1-day workshops aligned with characteristics of quality professional development are highly valued by participants and can improve teachers’ knowledge and efficacy, but teacher practice and student learning may be more difficult to influence and document.

Funder: Alzheimer Society of Canada; Id: http://dx.doi.org/10.13039/501100000143, Funder: Weston Brain Institute; Id: http://dx.doi.org/10.13039/100012479, Funder: Fonds de Recherche du Québec ‐ Santé, Funder: Canadian Institutes of Health Research; Id: http://dx.doi.org/10.13039/501100000024, Funder: NIHR Rare Diseases Translational Research Collaboration, Funder: Deutsche Forschungsgemeinschaft; Id: http://dx.doi.org/10.13039/501100001659, Recent studies have reported early cerebellar and subcortical impact in the disease progression of genetic frontotemporal dementia (FTD) due to microtubule-associated protein tau (MAPT), progranulin (GRN) and chromosome 9 open reading frame 72 (C9orf72). However, the cerebello-subcortical circuitry in FTD has been understudied despite its essential role in cognition and behaviors related to FTD symptomatology. The present study aims to investigate the association between cerebellar and subcortical atrophy, and neuropsychiatric symptoms across genetic mutations. Our study included 983 participants from the Genetic Frontotemporal dementia Initiative including mutation carriers and noncarrier first-degree relatives of known symptomatic carriers. Voxel-wise analysis of the thalamus, striatum, globus pallidus, amygdala, and the cerebellum was performed, and partial least squares analyses (PLS) were used to link morphometry and behavior. In presymptomatic C9orf72 expansion carriers, thalamic atrophy was found compared to noncarriers, suggesting the importance of this structure in FTD prodromes. PLS analyses demonstrated that the cerebello-subcortical circuitry is related to neuropsychiatric symptoms, with significant overlap in brain/behavior patterns, but also specificity for each genetic mutation group. The largest differences were in the cerebellar atrophy (larger extent in C9orf72 expansion group) and more prominent amygdalar volume reduction in the MAPT group. Brain scores in the C9orf72 expansion carriers and MAPT carriers demonstrated covariation patterns concordant with atrophy patterns detectable up to 20 years before expected symptom onset. Overall, these results demonstrated the important role of the subcortical structures in genetic FTD symptom expression, particularly the cerebellum in C9orf72 and the amygdala in MAPT carriers.

This study rereads Qoheleth and the Babylonian Theodicy through a trauma lens as opposed to the generic lens of speculative wisdom encompassing retributive justice, retribution theology, and the deed-consequence nexus and their consequent view of the gods’/God’s justice. According to retribution theology, actions correlate appropriately to consequences; however, in light of their suffering and resilience, both authors are disillusioned, struggling to make sense of life’s predicaments despite their religiosity and placating of the deity, as they also resist retribution theology. Rereading Qoheleth and the Babylonian Theodicy through a trauma lens shows that both sufferers seek answers and cope with suffering similarly to modern readers. This study acknowledges the use and limits of retribution theology operating within the Hebrew and Mesopotamian worldview to illustrate how these texts resist retributive justice. However, we also illustrate how the collective and individual aspects of trauma theory transcend ethic, cultural, epochal, and geographical boundaries. Furthermore, the juxtaposed ancient texts demonstrate how to cope with traumatic life experiences outside the confines of retributive justice by making meaning of self and others in the world and exhibiting resilience through effective coping strategies. Ultimately, Qoheleth affirms that while fearing God is right, navigating trauma includes enjoying God’s simple gifts such as food, wine, work, and a spouse for momentary relief. The Babylonian Theodicy sufferer makes meaning and develops resiliency through self-disclosure to friends and acceptance.

Background Compared to white individuals, Black and Hispanic individuals have higher rates of COVID-19 hospitalization and death. Less is known about racial/ethnic differences in post-acute sequelae of SARS-CoV-2 infection (PASC). Objective Examine racial/ethnic differences in potential PASC symptoms and conditions among hospitalized and non-hospitalized COVID-19 patients. Design Retrospective cohort study using data from electronic health records. Participants 62,339 patients with COVID-19 and 247,881 patients without COVID-19 in New York City between March 2020 and October 2021. Main Measures New symptoms and conditions 31–180 days after COVID-19 diagnosis. Key Results The final study population included 29,331 white patients (47.1%), 12,638 Black patients (20.3%), and 20,370 Hispanic patients (32.7%) diagnosed with COVID-19. After adjusting for confounders, significant racial/ethnic differences in incident symptoms and conditions existed among both hospitalized and non-hospitalized patients. For example, 31–180 days after a positive SARS-CoV-2 test, hospitalized Black patients had higher odds of being diagnosed with diabetes (adjusted odds ratio [OR]: 1.96, 95% confidence interval [CI]: 1.50—2.56, qq=0.02), compared to hospitalized white patients. Hospitalized Hispanic patients had higher odds of headaches (OR: 1.62, 95% CI: 1.21—2.17, q=0.003) and dyspnea (OR: 1.22, 95% CI: 1.05—1.42, q=0.02), compared to hospitalized white patients. Among non-hospitalized patients, Black patients had higher odds of being diagnosed with pulmonary embolism (OR: 1.68, 95% CI: 1.20—2.36, q=0.009) and diabetes (OR: 2.13, 95% CI: 1.75—2.58, qqqq < 0.001), but lower odds of encephalopathy (OR: 0.64, 95% CI: 0.51—0.80, q Conclusions Compared to white patients, patients from racial/ethnic minority groups had significantly different odds of developing potential PASC symptoms and conditions. Future research should examine the reasons for these differences.

The synthesis of new proteins and the degradation of old proteins in vivo can be quantified in serial samples using metabolic isotope labeling to measure turnover. Because serial biopsies in humans are impractical, we set out to develop a method to calculate the turnover rates of proteins from single human biopsies. This method involved a new metabolic labeling approach and adjustments to the calculations used in previous work to calculate protein turnover. We demonstrate that using a nonequilibrium isotope enrichment strategy avoids the time dependent bias caused by variable lag in label delivery to different tissues observed in traditional metabolic labeling methods. Turnover rates are consistent for the same subject in biopsies from different labeling periods, and turnover rates calculated in this study are consistent with previously reported values. We also demonstrate that by measuring protein turnover we can determine where proteins are synthesized. In human subjects a significant difference in turnover rates differentiated proteins synthesized in the salivary glands versus those imported from the serum. We also provide a data analysis tool, DeuteRater-H, to calculate protein turnover using this nonequilibrium metabolic

Extreme heat events are a threat to human health, productivity, and food supply, so understanding their drivers is critical to adaptation and resilience. Anticyclonic circulation and certain quasi-stationary Rossby wave patterns are well known to coincide with heatwaves, and soil moisture deficits amplify extreme heat in some regions. However, the relative roles of these two factors in causing heatwaves is still unclear. Here we use constructed circulation analogs to estimate the contribution of atmospheric circulation to heatwaves in the United States in the Community Earth System Model version 1 (CESM1) preindustrial control simulations. After accounting for the component of the heatwaves explained by circulation, we explore the relationship between the residual temperature anomalies and soil moisture. We find that circulation explains over 85% of heatwave temperature anomalies in the eastern and western United States but only 75%–85% in the central United States. In this region, there is a significant negative correlation between soil moisture the week before the heatwave and the strength of the heatwave that explains additional variance. Further, for the hottest central U.S. heatwaves, positive temperature anomalies and negative soil moisture anomalies are evident over a month before heatwave onset. These results provide evidence that positive land–atmosphere feedbacks may be amplifying heatwaves in the central United States and demonstrate the geographic heterogeneity in the relative importance of the land and atmosphere for heatwave development. Analysis of future circulation and soil moisture in the CESM1 Large Ensemble indicates that, over parts of the United States, both may be trending toward greater heatwave likelihood.

Hybridization caused by frequent environmental changes can lead both to species diversification (speciation) and to speciation reversal (despeciation), but the latter has rarely been demonstrated. Parthenocissus, a genus with its trifoliolate lineage in the Himalayan-Hengduan Mountains (HHM) region showing perplexing phylogenetic relationships, provides an opportunity for investigating speciation dynamics based on integrated evidence.We investigated phylogenetic discordance and reticulate evolution in Parthenocissus based on rigorous analyses of plastome and transcriptome data. We focused on reticulations in the trifoliolate lineage in the HHM region using a population-level genome resequencing dataset, incorporating evidence from morphology, distribution, and elevation.Comprehensive analyses confirmed multiple introgressions within Parthenocissus in a robust temporal-spatial framework. Around the HHM region, at least three hybridization hot spots were identified, one of which showed evidence of ongoing speciation reversal.We present a solid case study using an integrative methodological approach to investigate reticulate evolutionary history and its underlying mechanisms in plants. It demonstrates an example of speciation reversal through frequent hybridizations in the HHM region, which provides new perspectives on speciation dynamics in mountainous areas with strong topographic and environmental heterogeneity. info:eu-repo/semantics/publishedVersion

To examine racial and ethnic differences in maternal social support in infancy and the relationship between social support and mother-infant health behaviors.Secondary analysis of baseline data from a multisite obesity prevention trial that enrolled mothers and their 2-month-old infants. Behavioral and social support data were collected via questionnaire. We used modified Poisson regression to determine association between health behaviors and financial and emotional social support, adjusted for sociodemographic characteristics.Eight hundred and twenty-six mother-infant dyads (27.3% non-Hispanic Black, 18.0% Non-Hispanic White, 50.1% Hispanic and 4.6% Non-Hispanic Other). Half of mothers were born in the United States; 87% were Medicaid-insured. There were no racial/ethnic differences in social support controlling for maternal nativity. US-born mothers were more likely to have emotional and financial support (rate ratio [RR] 1.14 95% confidence interval [CI]: 1.07, 1.21 and RR 1.23 95% CI: 1.11, 1.37, respectively) versus mothers born outside the United States. Mothers with financial support were less likely to exclusively feed with breast milk (RR 0.62; 95% CI: 0.45, 0.87) yet more likely to have tummy time ≥12min (RR 1.28; 95% CI: 1.02, 1.59) versus mothers without financial support. Mothers with emotional support were less likely to report feeding with breast milk (RR 0.82; 95% CI: 0.69, 0.97) versus mothers without emotional support.Nativity, not race or ethnicity, is a significant determinant of maternal social support. Greater social support was not universally associated with healthy behaviors. Interventions may wish to consider the complex nature of social support and population-specific social support needs.

Aim: This retrospective, observational study assessed healthcare resource utilization (HCRU) and costs for newly diagnosed acute myeloid leukemia (AML) patients receiving intensive induction chemotherapy. Materials & methods: Adult AML patients with inpatient hospitalization or hospital-based outpatient visit receiving intensive induction chemotherapy (CPX-351 or 7 + 3 treatments) were identified from the Premier Healthcare Database (US). Results: All 642 patients had inpatient hospitalizations (median number = 2; median length of stay = 16 days); 22.4% had an ICU admission. Median total outpatient hospital cost was US$2904 per patient, inpatient hospital cost was $83,440 per patient, and ICU cost was $16,550 per patient. Discussion: In the US hospital setting, substantial HCRU and costs associated with intensive induction chemotherapy for AML were driven by inpatient hospitalizations.

Controversy over a reported increase in office visits specifically scheduled for illnesses and conditions in children has stalled progress in understanding adverse outcomes associated with an increasingly crowded schedule of pediatric vaccines. Studies finding associations between vaccines and adverse conditions have been targeted for retraction. Here, we revisit data from one such study, comparing the increase in office visits for conditions independent of the routine “well-child” visits (hereafter, Health Care Visits; HCVs). The retraction occurred after >1/4 of a million people had read the peer-reviewed study. It was targeted by one anonymous reader who complained he did not believe the published results. His complaint hinged on the supposition — unsupported by any data — that vaccinated children made their scheduled HCVs more regularly than unvaccinated, implying that those unkept appointments led to fewer diagnoses. We show, here, new data from the same practice that the opposite is true. When the data for vaccinated versus unvaccinated children are examined, the critic’s claim is exactly reversed. Relative Risk and Odds Ratios sustain and augment the original report. Additional office visits, beyond scheduled HCVs, are quantified, controlling for variation in kept HCVs and age/days of care. Estimates of Health Care Incidence (HCI) show that visits above regular HCVs increase due to vaccination by 2.56 to 4.98 per year. Blocking and multiple linear regression analysis of interactions indicate both that the unvaccinated are keeping scheduled HCVs more often than the vaccinated, and that vaccination comes with a net increase in non-routine office visits, i.e., not “well-baby visits” but trips to the doctor for reasons other than vaccination. Taking account of the complexities of healthcare-seeking with measured covariates and outcomes, especially adverse health events, suggests that vaccination may be driving the increased need for non-routine office visits for specific health complaints. Meanwhile, one reader’s unsupported and false criticism of the former study, reflects a pervasive bias leading to systematic removal of many well-designed studies attributing adverse outcomes to vaccines. Hiding such well-designed and faithfully reported, not to mention peer-reviewed and published research, clears the way for marketing programs bought and paid for by vaccine manufacturers and the Centers for Disease Control and Prevention (CDC).

The following is being published as if it were a “Letter to the Editor”[1] of the IJVTPR. It was written in response to a request to the Editorial Board from Charles Tortise on behalf of the de jure Sovereign Wakaminenga Maori Government (WMG) of Nui Tireni New Zealand. He called on the journal editors and authors to supply up to the minute information to be used in helping to shape nationwide policy in New Zealand during the COVID-19 Aftermath. Whereas the leader of the de facto Wellington government, the Prime Minister Jacinda Ardern, recently relaxed certain “mandates” — ones that Blaylock refers to as “draconian” — concerning the COVID-19 masks and injections, her whole de facto NZ government, which draws authority from the 1840 Treaty of Waitangi has always been legally subordinated to the de jure Sovereign Wakaminenga, in accordance with the 1835 He Wakaputanga, a Declaration of Independence by the Confederation of the Chiefs of the United Maori tribes. By law, the subordinate NZ government headed up now by Ardern, must be authorized each year by the agreement of the direct descendants of those Chiefs, meeting in Congress, known as the National Wakaminenga, to continue as the de facto government. This being the case, it is the declared intention of the WMG to learn as much as possible about the COVID-19 injectables, and about the world-wide genetic experiments that are underway. Such information is needed in order for the de jure WMG to decide wisely about whether the policies and regulations put in place as the COVID-19 response of the de facto NZ government in Wellington were as “safe and effective” as has been said and review their performance accordingly. Maori are defined as people who “aspire to purity without blemish”, and the jurisdiction they have is, as far as we know, unique in all the world. Therefore, the discussion underway there, incorporating the information in this letter, written by Russell Blaylock, MD and retired neurosurgeon, is addressed not only to the WMG through Charles G. Tortise, but also the whole world. It is written on behalf of a group of people hardly known to much of the rest of the world but who are, in the estimation of the editors of this journal, about to make world-wide history in respect to the COVID Aftermath. It was after consultation among several members of our Editorial Board that we decided to call on Russell Blaylock, to write the initial position paper, as it were, to be presented to the WMG. He won’t say it but we will: he is eminently well qualified and credentialed to write the opinion letter that follows. This is his position paper for the WMG. [1] This letter has been reviewed by three other members of the Editorial Board for the IJVTPR and is published here because of the importance of the issues at stake not only to all New Zealand, but to the whole world. In the opinion of the editors, the policies being challenged by the sovereign Wakaminenga Maori Government (WMG) of New Zealand — for reasons detailed by Russell Blaylock, MD — are of critical importance to the whole world. The WMG is led by Arikinui Ripekatangi also known by her English name as Georgina Job. The term “Wakaminenga” in Te reo, the Maori language, means “assembly”. The Maori people are from different tribes called “iwi” and smaller groups known as “hapu”. Leaders of the northern tribes began meeting from about 1808 in a formal assembly to discuss laws and policy concerning the increasing interactions with foreigners, especially the British that followed soon after James Cook landed there during one of his voyages of exploration. The assembly was known as “Te Wakaminenga o Ngā Hapū o Nu Tīreni” [the General Assembly of the Tribal Nations]. The WMG is the administrative arm of the current National Assembly, website here. Of note Arikinui Ripekatangi issued a statement dated August 16, 2022 outlining the background leading to this article. The website address for the WMG news releases is here.

Injury : international journal of the care of the injured 53(8), 2774-2782 (2022). doi:10.1016/j.injury.2022.05.009, Published by Elsevier Science, Amsterdam [u.a.]

Examine factors impacting U.S. parents' intention to vaccinate their children against COVID-19.Data were collected February-May 2021 from parents living in six geographically diverse locations. The COVID-19 Exposure and Family Impact Survey assessed perceived susceptibility and severity to adverse outcomes from the pandemic. Semi-structured interviews assessed perceptions about benefits and risks of vaccinating children.Fifty parents of 106 children (newborn-17 years) were included; half were Spanish-speaking and half English-speaking. 62% were hesitant about vaccinating their children against COVID-19. Efficacy and safety were the main themes that emerged: some parents perceived them as benefits while others perceived them as risks to vaccination. Parent hesitancy often relied on social media, and was influenced by narrative accounts of vaccination experiences. Many cited the lower risk of negative outcomes from COVID-19 among children, when compared with adults. Some also cited inaccurate and constantly changing information about COVID-19 vaccines.Main drivers of parent hesitancy regarding child COVID-19 vaccination include perceived safety and efficacy of the vaccines and lower severity of illness in children.Many vaccine-hesitant parents may be open to vaccination in the future and welcome additional discussion and data.

This study employs qualitative methodology to assess surgeons' perspective on decision making in management of recurrent diverticulitis to improve patient-centered decision making.The decision to pursue colectomy for patients with recurrent diverticulitis is nuanced. Strategies to enact broad acceptance of guidelines for surgery are hindered because of a knowledge gap in understanding surgeons' current attitudes and opinions.We performed semi-structured interviews with board-certified North American general and colorectal surgeons who manage recurrent diverticulitis. We purposely sampled specialists by both surgeon and practice factors. An iterative inductive/deductive strategy was used to code and analyze the interviews and create a conceptual framework.25 surgeons were enrolled over a nine-month period. There was diversity in surgeons' gender, age, experience, training, specialty (colorectal vs general surgery) and geography. Surgeons described the difficult process to determine who receives an operation. We identified seven major themes as well as twenty subthemes of the decision-making process. These were organized into a conceptual model. Across the spectrum of interviews, it was notable that there was a move over time from decisions based on counting episodes of diverticulitis to a focus on improving quality of life. Surgeons also felt that quality of life was more dependent on psychosocial factors than the degree of physiological dysfunction. [What about what surprised you/].Surgeons mostly have discarded older dogma in recommending colectomy for recurrent diverticulitis based on number and severity of episodes. Instead, decision making in recurrent diverticulitis is complex, involving multiple surgeon and patient factors and evolving over time. Surgeons struggle with this decision and education- or communication-based interventions that focus on shared decision making warrant development.

Four-dimensional COAMPS Dynamic Initialization (FCDI) analyses with high temporal and spatial resolution GOES-16 Atmospheric Motion Vectors (AMVs) are utilized to analyze the development and rapid intensification of a mesovortex about 150 km to the south of the center of the subtropical cyclone Henri (2021). During the period of the unusual Henri westward track along 30°N, the FCDI z = 300 m wind vector analyses demonstrate highly asymmetric wind fields and a horse-shoe shaped isotach maximum that is about 75 km from the center, which are characteristics more consistent with the definition of a subtropical cyclone than of a tropical cyclone. Furthermore, the Henri westward track and the vertical wind shear have characteristics resembling a Rossby Wave Breaking conceptual model. The GOES-16 mesodomain AMVs allow the visualization of a series of outflow bursts in space and time in association with the southern mesovortex development and intensification. Then the FCDI analyses forced by those thousands of AMVs each 15 minutes depict the z = 13,910 m wind field responses and the subsequent z = 300 m wind field adjustments in the southern mesovortex. A second northern outflow burst displaced to the southeast of the main Henri vortex also led to a strong low-level mesovortex. It was when the two outflow bursts joined to create an eastward radial outflow all along the line between them that the southern mesovortex reached maximum intensity and maximum size. In contrast to the numerical model predictions of intensification, outflow from the mesovortex directed over the main Henri vortex led to a decrease in intensity.

© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/., There is a strong genetic contribution to Amyotrophic lateral sclerosis (ALS) risk, with heritability estimates of up to 60%. Both Mendelian and small effect variants have been identified, but in common with other conditions, such variants only explain a little of the heritability. Genomic structural variation might account for some of this otherwise unexplained heritability. We therefore investigated association between structural variation in a set of 25 ALS genes, and ALS risk and phenotype. As expected, the repeat expansion in the C9orf72 gene was identified as associated with ALS. Two other ALS-associated structural variants were identified: inversion in the VCP gene and insertion in the ERBB4 gene. All three variants were associated both with increased risk of ALS and specific phenotypic patterns of disease expression. More than 70% of people with respiratory onset ALS harboured ERBB4 insertion compared with 25% of the general population, suggesting respiratory onset ALS may be a distinct genetic subtype., This research was funded in whole, or in part, by the Wellcome Trust [Grant number]. For the purpose of open access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission. The authors acknowledge use of the research computing facility at King’s College London, Rosalind (https://rosalind.kcl.ac.uk), which is delivered in partnership with the National Institute for Health Research (NIHR) Biomedical Research Centres at South London & Maudsley and Guy’s & St. Thomas’ NHS Foundation Trusts, and part-funded by capital equipment grants from the Maudsley Charity (award 980) and Guy’s & St. Thomas’ Charity (TR130505). The views expressed are those of the author (s) and not necessarily those of the NHS, the NIHR, King’s College London, or the Department of Health and Social Care. National. The authors acknowledge Institute for Health Research (NIHR) Biomedical Research Centre at South London and Maudsley NHS Foundation Trust and King’s College London. The authors acknowledge Health Data Research UK, which is funded by the UK Medical Research Council, Engineering and Physical Sciences Research Council, Economic and Social Research Council, Department of Health and Social Care (England), Chief Scientist Office of the Scottish Government Health and Social Care Directorates, Health and Social Care Research and Development Division (Welsh Government), Public Health Agency (Northern Ireland), British Heart Foundation and Wellcome Trust. A.A.K. is funded by The Motor Neurone Disease Association (MNDA), NIHR Maudsley Biomedical Research Centre and and ALS Association Milton Safenowitz Research Fellowship. This project was funded by the MND Association and the Wellcome Trust. This is an EU Joint Programme-Neurodegenerative Disease Research (JPND) project. The project is supported through the following funding organisations under the aegis of JPND - www.jpnd.eu (United Kingdom, Medical Research Council (MR/L501529/1 and MR/R024804/1) and Economic and Social Research Council (ES/L008238/1)). A.A.C. is an NIHR Senior Investigator. C.E.S. and A.A.C. receive salary support from the National Institute for Health Research (NIHR) Dementia Biomedical Research Unit at South London and Maudsley NHS Foundation Trust and King’s College London. The views expressed are those of the authors and not necessarily those of the NHS, the NIHR or the Department of Health. The work leading up to this publication was funded by the European Community’s Health Seventh Framework Program (FP7/2007–2013; grant agreement number 259867) and Horizon 2020 Program (H2020-PHC-2014-two-stage; grant agreement number 633413). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 772376 - EScORIAL. The collaboration project is co-funded by the PPP Allowance made available by Health~Holland, Top Sector Life Sciences & Health, to stimulate public-private partnerships. Project MinE Belgium was supported by a grant from IWT, the Belgian ALS Liga and a grant from Opening the Future Fund (KU Leuven). PVD holds a senior clinical investigatorship of FWO-Vlaanderen and is supported by E. von Behring Chair for Neuromuscular and Neurodegenerative Disorders, the ALS Liga België and the KU Leuven funds “Een Hart voor ALS”, “Laeversfonds voor ALS Onderzoek” and the “Valéry Perrier Race against ALS Fund”. R.L.McL. is supported by Science Foundation Ireland (17/CDA/4737). MinE USA is funded by the US ALS Association. We thank the patients and families from the Emory ALS Clinic participating in this research. Funding was provided by US National Institutes of Health (NIH)/National Institute of Neurological Disorders and Stroke (NINDS) (R01NS073873, J.E.L.) and the American ALS Association (J.E.L.).

Morphological and single-nucleotide polymorphism data support splitting Pultenaea glabra Benth. into eight species, including one in Victoria, and seven in eastern and northern New South Wales. Six species are newly described, five of which are, like P. glabra, narrow-range endemics within the Greater Blue Mountains World Heritage Area and adjacent sandstone landforms of the Great Dividing Range. The recognition of six new species from what was broadly P. glabra has implications for conservation management, including for P. glabra itself, which has a smaller distribution and more precise habitat requirements than previously thought. One of the new species, P. percussa, is known by a single 1971 gathering only. The occurrence of several narrow-range Blue Mountains endemic Pultenaea species may be explained by the combination of edaphic diversity and topographic complexity, which could act in concert to promote divergences among small, allopatric populations. Hybrids between P. glabra and P. flexilis Sm. are documented for the first time; however, limited evidence for introgression between the two species was observed. The following new taxa are described: Pultenaea aculeata M.A.M.Renner, P.H.Weston & S.Clarke, Pultenaea percussa M.A.M.Renner & P.H.Weston, Pultenaea furcata M.A.M.Renner & R.L.Barrett, Pultenaea mutabilis M.A.M.Renner & P.H.Weston, Pultenaea mutabilis var. angusta M.A.M.Renner, P.H.Weston, & S.Clarke, Pultenaea praecipua M.A.M.Renner & P.H.Weston, Pultenaea praecipua subsp. temperata M.A.M.Renner & R.L.Barrett, and Pultenaea tenebrosa M.A.M.Renner, P.H.Weston & S.Clarke. Lectotypes are designated for Pultenaea villosa var. glabrescens Benth. and Pultenaea weindorferi Reader.

OBJECTIVE We examined users' preferences for and engagement with text messages delivered as part of an emergency department (ED)-based intervention to improve antihypertensive medication adherence. METHODS We recruited ED patients with elevated blood pressure for a pilot randomized trial evaluating a medication adherence intervention with text messages. Intervention participants chose text content and frequency, received texts for 45 days, and completed a feedback survey. We defined engagement via responses to texts. We examined participant characteristics associated with text preferences, engagement, and feedback. RESULTS Participants (N = 101) were 57% female and 46% non-White. Most participants (71%) chose to receive both reminder and informational texts; 94% chose reminder texts once per day and 97% chose informational texts three times per week. Median text message response rate was 56% (IQR 26-80%). Participants who were Black (p

Earth's ecosystems are increasingly threatened by "hot drought," which occurs when hot air temperatures coincide with precipitation deficits, intensifying the hydrological, physiological, and ecological effects of drought by enhancing evaporative losses of soil moisture (SM) and increasing plant stress due to higher vapor pressure deficit (VPD). Drought-induced reductions in gross primary production (GPP) exert a major influence on the terrestrial carbon sink, but the extent to which hotter and atmospherically drier conditions will amplify the effects of precipitation deficits on Earth's carbon cycle remains largely unknown. During summer and autumn 2020, the U.S. Southwest experienced one of the most intense hot droughts on record, with record-low precipitation and record-high air temperature and VPD across the region. Here, we use this natural experiment to evaluate the effects of hot drought on GPP and further decompose those negative GPP anomalies into their constituent meteorological and hydrological drivers. We found a 122 Tg C (>25%) reduction in GPP below the 2015-2019 mean, by far the lowest regional GPP over the Soil Moisture Active Passive satellite record. Roughly half of the estimated GPP loss was attributable to low SM (likely a combination of record-low precipitation and warming-enhanced evaporative depletion), but record-breaking VPD amplified the reduction of GPP, contributing roughly 40% of the GPP anomaly. Both air temperature and VPD are very likely to continue increasing over the next century, likely leading to more frequent and intense hot droughts and substantially enhancing drought-induced GPP reductions.

Recent studies have investigated post-acute sequelae of SARS-CoV-2 infection (PASC, or long COVID) using real-world patient data such as electronic health records (EHR). Prior studies have typically been conducted on patient cohorts with specific patient populations which makes their generalizability unclear. This study aims to characterize PASC using the EHR data warehouses from two large Patient-Centered Clinical Research Networks (PCORnet), INSIGHT and OneFlorida+, which include 11 million patients in New York City (NYC) area and 16.8 million patients in Florida respectively. With a high-throughput screening pipeline based on propensity score and inverse probability of treatment weighting, we identified a broad list of diagnoses and medications which exhibited significantly higher incidence risk for patients 30–180 days after the laboratory-confirmed SARS-CoV-2 infection compared to non-infected patients. We identified more PASC diagnoses in NYC than in Florida regarding our screening criteria, and conditions including dementia, hair loss, pressure ulcers, pulmonary fibrosis, dyspnea, pulmonary embolism, chest pain, abnormal heartbeat, malaise, and fatigue, were replicated across both cohorts. Our analyses highlight potentially heterogeneous risks of PASC in different populations.

Background Bulbar dysfunction is a cardinal feature of ALS with important quality of life and management implications. The objective of this study is the longitudinal evaluation of a large panel imaging metrics pertaining to bulbar dysfunction, encompassing cortical measures, structural and functional cortico-medullary connectivity indices and brainstem metrics. Methods A standardised, multimodal imaging protocol was implemented with clinical and genetic profiling to systematically appraise the biomarker potential of specific metrics. A total of 198 patients with ALS and 108 healthy controls were included. Results Longitudinal analyses revealed progressive structural and functional disconnection between the motor cortex and the brainstem over time. Cortical thickness reduction was an early feature on cross-sectional analyses with limited further progression on longitudinal follow-up. Receiver operating characteristic analyses of the panel of MR metrics confirmed the discriminatory potential of bulbar imaging measures between patients and controls and area-under-the-curve values increased significantly on longitudinal follow-up. C9orf72 carriers exhibited lower brainstem volumes, lower cortico-medullary structural connectivity and faster cortical thinning. Sporadic patients without bulbar symptoms, already exhibit significant brainstem and cortico-medullary connectivity alterations. Discussion Our results indicate that ALS is associated with multi-level integrity change from cortex to brainstem. The demonstration of significant corticobulbar alterations in patients without bulbar symptoms confirms considerable presymptomatic disease burden in sporadic ALS. The systematic assessment of radiological measures in a single-centre academic study helps to appraise the diagnostic and monitoring utility of specific measures for future clinical and clinical trial applications.

OBJECTIVESpinocerebellar ataxia type 12 (SCA12) is a neurodegenerative disease caused by expansion of a CAG repeat in thePPP2R2B gene. Here we tested the hypothesis that thePPP2R2B antisense(PPP2R2B-AS1) transcript containing a CUG repeat is expressed and contributes to SCA12 pathogenesis.METHODSExpression ofPPP2R2B-AS1transcript was detected in SCA12 human induced pluripotent stem cells (iPSCs), iPSC-derived NGN2 neurons, and SCA12 knock-in mouse brains using strand-specific RT-PCR (SS-RT-PCR). The tendency of expandedPPP2R2B-AS1(expPPP2R2B-AS1) RNA to form foci, a marker of toxic processes involving mutant RNAs, was examined in SCA12 cell models by fluorescencein situhybridization. The toxic effect ofexpPPP2R2B-AS1transcripts on SK-N-MC neuroblastoma cells was evaluated by caspase 3/7 activity. Western blot was used to examine the expression of repeat associated non-ATG-initiated (RAN) translation ofexpPPP2R2B-AS1transcript in SK-N-MC cells.RESULTSThe repeat region inPPP2R2Bgene locus is bidirectionally transcribed in SCA12 iPSCs, iPSC-derived NGN2 neurons, and SCA12 mouse brains. TransfectedexpPPP2R2B-AS1transcripts are toxic to SK-N-MC cells, and the toxicity may be mediated, at least in part, by the RNA secondary structure. TheexpPPP2R2B-AS1transcripts form CUG RNA foci in SK-N-MC cells.expPPP2R2B-AS1transcript is translated in the Alanine ORF via repeat-associated non-ATG (RAN) translation, which is diminished by single nucleotide interruptions within the CUG repeat, and MBNL1 overexpression.INTERPRETATIONThese findings suggest thatPPP2R2B-AS1contributes to SCA12 pathogenesis, and may therefore provide a novel therapeutic target for the disease.

ObjectivesTo determine whether hydroxychloroquine (HCQ) is safe and effective at preventing COVID-19 infections among health care workers (HCWs).MethodsIn a 1: 1 randomized, placebo-controlled, double-blind, parallel-group, superiority trial at 34 US clinical centers, 1360 HCWs at risk for COVID-19 infection were enrolled between April and November 2020. Participants were randomized to HCQ or matched placebo. The HCQ dosing included a loading dose of HCQ 600 mg twice on day 1, followed by 400 mg daily for 29 days. The primary outcome was a composite of confirmed or suspected COVID-19 clinical infection by day 30, defined as new-onset fever, cough, or dyspnea and either a positive SARS-CoV-2 polymerase chain reaction test (confirmed) or a lack of confirmatory testing due to local restrictions (suspected).ResultsStudy enrollment closed before full accrual due to recruitment challenges. The primary end point occurred in 41 (6.0%) participants receiving HCQ and 53 (7.8%) participants receiving placebo. No difference in the proportion of participants experiencing clinical infection (estimated difference of -1.8%, 95% confidence interval -4.6-0.9%, P=0.20) was identified nor any significant safety issues.ConclusionOral HCQ taken as prescribed appeared safe among HCWs. No significant clinical benefits were observed. The study was not powered to detect a small but potentially important reduction in infection.Trial registrationNCT04334148.

This article takes its point of departure in a puzzle in the little studied De generatione et corruptione commentary by Spanish Dominican Domingo Báñez, first published in 1585. Specifically, five times in the work, Báñez takes as his opponent an otherwise unidentified novus philosophus or novus author. Using parallel texts, I show Báñez’s novus philosophus was none other than the Jesuit Franciscus Toletus. I then trace some of the background to one of the issues on which Báñez criticizes Franciscus: the issue of whether or not human rational souls are equal to one another in terms of substantial perfection. The central place of Thomas de Vio Cajetan in the dispute between the Dominican and the Jesuit quickly becomes clear. In an appendix, I offer complete question lists of the relevant natural philosophical works by Franciscus from which Báñez quotes or paraphrases, i.e. Franciscus’s Physics, De generatione, and De anima commentaries. Link to article: https://journals.uco.es/mediterranea/article/view/15643/14395 ispartof: Mediterranea vol:8 pages:595-634 status: Published online

BackgroundSoil-derived prokaryotic gut communities of the Japanese beetle Popillia japonica Newman (JB) larval gut include heterotrophic, ammonia-oxidizing, and methanogenic microbes potentially capable of promoting greenhouse gas (GHG) emissions. However, no research has directly explored GHG emissions or the eukaryotic microbiota associated with the larval gut of this invasive species. In particular, fungi are frequently associated with the insect gut where they produce digestive enzymes and aid in nutrient acquisition. Using a series of laboratory and field experiments, this study aimed to (1) assess the impact of JB larvae on soil GHG emissions; (2) characterize gut mycobiota associated with these larvae; and (3) examine how soil biological and physicochemical characteristics influence variation in both GHG emissions and the composition of larval gut mycobiota.MethodsManipulative laboratory experiments consisted of microcosms containing increasing densities of JB larvae alone or in clean (uninfested) soil. Field experiments included 10 locations across Indiana and Wisconsin where gas samples from soils, as well as JB and their associated soil were collected to analyze soil GHG emissions, and mycobiota (ITS survey), respectively.ResultsIn laboratory trials, emission rates of CO2, CH4, and N2O from infested soil were ≥ 6.3× higher per larva than emissions from JB larvae alone whereas CO2 emission rates from soils previously infested by JB larvae were 1.3× higher than emissions from JB larvae alone. In the field, JB larval density was a significant predictor of CO2 emissions from infested soils, and both CO2 and CH4 emissions were higher in previously infested soils. We found that geographic location had the greatest influence on variation in larval gut mycobiota, although the effects of compartment (i.e., soil, midgut and hindgut) were also significant. There was substantial overlap in the composition and prevalence of the core fungal mycobiota across compartments with prominent fungal taxa being associated with cellulose degradation and prokaryotic methane production/consumption. Soil physicochemical characteristics such as organic matter, cation exchange capacity, sand, and water holding capacity, were also correlated with both soil GHG emission, and fungal a-diversity within the JB larval gut. Conclusions: Results indicate JB larvae promote GHG emissions from the soil directly through metabolic activities, and indirectly by creating soil conditions that favor GHG-associated microbial activity. Fungal communities associated with the JB larval gut are primarily influenced by adaptation to local soils, with many prominent members of that consortium potentially contributing to C and N transformations capable of influencing GHG emissions from infested soil.

OBJECTIVE: Clozapine is the most efficacious antipsychotic medication, but it is underutilized and its mechanism of action is still poorly understood. One aspect of its unique efficacy that requires further study is its effect on suicidality. A randomized controlled trial, the InterSePT study, yielded evidence that clozapine reduces suicidality more than olanzapine, after which it became the only medication indicated for recurrent suicidal behavior in schizophrenia and schizoaffective disorder. We present here the first study of population mortality data to investigate the effect of clozapine on suicide. METHODS: We reviewed statewide autopsy records of Maryland’s Office of the Chief Medical Examiner, which performs uniquely comprehensive death investigations that include full toxicologic panels with postmortem blood levels of antipsychotics. Our study compared clozapine- and olanzapine-positive decedents across demographic, clinical, and manner-of-death outcomes using contingency table analysis and logistic regression. RESULTS: Of 53,144 decedents from 2003 to 2021, 621 had clozapine or olanzapine detected on autopsy, with the two groups showing no demographic differences. Decedents with clozapine were significantly less likely to have died by suicide than by accident compared to those with olanzapine (odds ratio = 0.47; 95% CI, 0.26–0.84; P = .011). CONCLUSIONS: Our study thus adds more naturalistic evidence to the growing literature on the beneficial effect of clozapine on suicidality. Our findings also highlight the utility of statewide autopsy records, an untapped resource for investigating the potential protective effect of psychiatric medications on suicide at a population level.

ImportanceThe frequency and characteristics of post-acute sequelae of SARS-CoV-2 infection (PASC) may vary by SARS-CoV-2 variant.ObjectiveTo characterize PASC-related conditions among individuals likely infected by the ancestral strain in 2020 and individuals likely infected by the Delta variant in 2021.DesignRetrospective cohort study of electronic medical record data for approximately 27 million patients from March 1, 2020-November 30, 2021.SettingHealthcare facilities in New York and Florida.ParticipantsPatients who were at least 20 years old and had diagnosis codes that included at least one SARS-CoV-2 viral test during the study period.ExposureLaboratory-confirmed COVID-19 infection, classified by the most common variant prevalent in those regions at the time.Main Outcome(s) and Measure(s)Relative risk (estimated by adjusted hazard ratio [aHR]) and absolute risk difference (estimated by adjusted excess burden) of new conditions, defined as new documentation of symptoms or diagnoses, in persons between 31-180 days after a positive COVID-19 test compared to persons with only negative tests during the 31-180 days after the last negative test.ResultsWe analyzed data from 560,752 patients. The median age was 57 years; 60.3% were female, 20.0% non-Hispanic Black, and 19.6% Hispanic. During the study period, 57,616 patients had a positive SARS-CoV-2 test; 503,136 did not. For infections during the ancestral strain period, pulmonary fibrosis, edema (excess fluid), and inflammation had the largest aHR, comparing those with a positive test to those with a negative test, (aHR 2.32 [95% CI 2.09 2.57]), and dyspnea (shortness of breath) carried the largest excess burden (47.6 more cases per 1,000 persons). For infections during the Delta period, pulmonary embolism had the largest aHR comparing those with a positive test to a negative test (aHR 2.18 [95% CI 1.57, 3.01]), and abdominal pain carried the largest excess burden (85.3 more cases per 1,000 persons).Conclusions and RelevanceWe documented a substantial relative risk of pulmonary embolism and large absolute risk difference of abdomen-related symptoms after SARS-CoV-2 infection during the Delta variant period. As new SARS-CoV-2 variants emerge, researchers and clinicians should monitor patients for changing symptoms and conditions that develop after infection.STATEMENTS AND ACKNOWLEDGEMENTSAuthorship has been determined by ICJME recommendationDisclosures to be obtained at time of submissionThe content is solely the responsibility of the authors and does not necessarily represent the official views of the RECOVER Program, the NIH or other fundersWe would like to thank the National Community Engagement Group (NCEG), all patient, caregiver and community Representatives, and all the participants enrolled in the RECOVER Initiative.

Allen Lowrie memorial.

The production of commercial potatoes (Solanum tuberosum) for different market classes is of vital agricultural importance in the United States. For the production of chips, fresh-market or processing potatoes, potato producers rely upon different potato cultivars to meet market and consumer demands. Many cultivars possess distinctive traits which make them more or less susceptible to disease and insect pressure. One important and understudied trait that may confer host location preference and population performance include leaf fatty acid composition(s). It is known that leaf fatty acid composition can influence growth, taste, and even insect defense mechanisms. In the current investigation we examined the fatty acid composition of leaf tissue obtained from 8 commercial potato cultivars representing different market classes grown in a controlled field study. We examined colonization preference of Leptinotarsa decemlineata among the 8 different potato cultivars over a 45 day period representing the first full generation in Wisconsin. Results of these experiments suggest, i) that fatty acid compositions in leaf tissue vary between potato cultivars and among sample dates throughout the experiment, ii) that Leptinotarsa decemlineata may preferentially colonize particular potato cultivars, and iii) that there was a significant correlation between omega-3 fatty acid composition in leaf tissue and Leptinotarsa decemlineata cultivar preference.

Designing clinical trials on hemorrhage control requires carefully balancing the need for high enrollment numbers with the need of focusing on the sickest patients. The Pragmatic Randomized Optimal Platelet and Plasma Ratios (PROPPR) trial enrolled patients within 2 hours of arrival to the emergency department for a trial of injured patients at risk for massive transfusion. We conducted a secondary analysis to determine how time-to-randomization affected patient outcomes and the balance between enrollment and mortality.Patients from the Pragmatic Randomized Optimal Platelet and Plasma Ratios trial were compared based on 30-minute time to randomization intervals. Outcomes included 24-hour and 30-day mortality, time to hemostasis, adverse events, and operative procedures. Additional analyses were conducted based on treatment arm allocation, mechanism of injury, and variation in start time (arrival vs. randomization).Randomization within 30 minutes of arrival was associated with higher injury severity (median Injury Severity Score, 29 vs. 26 overall; p0.01), lower systolic blood pressure (median, 91 vs. 102 mm Hg overall; p0.01), and increased penetrating mechanism (50% vs. 47% overall; p0.01). Faster time-to-randomization was associated with increased 24-hour (20% for 0- to 30 minute entry, 9% for 31-minute to 60-minute entry, 10% for 61-minute to 90-minute entry, 0% for 91-minute to 120-minute entry; p0.01) and 30-day mortality (p0.01). There were no significant associations between time-to-randomization and adverse event occurrence, operative interventions, or time to hemostasis.Increasing time to randomization in this large multicenter randomized trial was associated with increased survival. Fastest randomization (within 0-30 minutes) was associated with highest 24-hour and 30-day mortality, but only 57% of patients were enrolled within this timeframe. Only 3% of patients were enrolled within the last 30-minute window (91-120 minutes), with none of them dying within the first 24 hours. For a more optimal balance between enrollment and mortality, investigators should consider shortening the time to randomization when planning future clinical trials of hemorrhage control interventions.Prognostic and Epidemiologic; Level II.

The timing of stroke onset among patients with blunt cerebrovascular injury (BCVI) is not well understood. All blunt trauma patients at our institution undergo a screening computed tomographic angiography (CTA) of the neck. Most patients with CTA evidence of BCVI are treated with aspirin, and all patients with clinical evidence of stroke are treated with aspirin and undergo magnetic resonance imaging (MRI) of the brain. We conducted a retrospective review to determine the incidence of stroke upon admission and following admission.All neck CTAs and head MRIs obtained in blunt trauma patients were reviewed from August 2017 to August 2019. All CTAs that were interpreted as showing BCVI were individually reviewed to confirm the diagnosis of BCVI. Stroke was defined as brain MRI evidence of new ischemic lesions, and each MRI was reviewed to identify the brain territory affected. We extracted the time to aspirin administration and the timing of stroke onset from patients' electronic health records.Of the 6,849 blunt trauma patients, 479 (7.0%) had BCVIs. Twenty-four patients (5.0%) with BCVI had a stroke on admission. Twelve (2.6%) of the remaining 455 patients subsequently had a stroke during their hospitalization. The incidence of stroke among patients with BCVI was 7.5%; 2.6% were potentially preventable. Only 5 of the 12 patients received aspirin before the onset of stroke symptoms. All 36 patients with BCVI and stroke had thromboembolic lesions in the territory supplied by an injured vessel.With universal screening, CTA evidence of BCVI is common among blunt trauma patients. Although acute stroke is also relatively common in this population, two thirds of strokes are already evident on admission. One third of BCVI-related strokes occur after admission and often relatively early, necessitating rapid commencement of preventative treatment. Further studies are required to demonstrate the value of antithrombotic administration in preventing stroke in BCVI patients.Prognostic and Epidemiologic; Level IV.