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Additional file 1: Figure S1. Selection diagram fortransportability analysis of the effects of stimulant use on initiation ofmedication for opioid use disorder (MOUD). Variables in gray text were notmeasured in one target population; those in gray boxes were not measured in anytarget population. Table S1. Inclusion andexclusion criteria, recruitment, screening and enrollment data for CTN clinicaltrials. Table S2. Inverse probabilityof selection weight models. Table S3.Distributions of inverse probability of selection weights and bias-variance tradeoffin weight trimming. Bias and precision of the stimulant use estimates arecomputed relative to untrimmed weights.

Additional file 8: Supplementary Table 1. Contingency table of microinjected litters analysed for fluorescence. Column variables: genotype of mice born after microinjection procedure. Row variables: observed fluorescence in the intestine, with at least two regions required to be deemed transduced. Numbers represent the count of mice. Chi-square (and Fisher’s exact) test was applied and a p-value >0.9999 was determined.

Phenotypic associations have been reported between blood cell traits (BCTs) and a range of neurological and psychiatric disorders (NPDs), but in most cases, it remains unclear whether these associations have a genetic basis and, if so, to what extent genetic correlations reflect causality. Here, we report genetic correlations and Mendelian randomization analyses between 11 NPDs and 29 BCTs, using genome-wide association study summary statistics. We found significant genetic correlations for four BCT-NPD pairs, all of which have prior evidence for a phenotypic correlation. We identified a previously unreported causal effect of increased platelet distribution width on susceptibility to Parkinson's disease. We identified multiple functional genes and regulatory elements for specific BCT-NPD pairs, some of which are targets of known drugs. These results enrich our understanding of the shared genetic landscape underlying BCTs and NPDs and provide a robust foundation for future work to improve prognosis and treatment of common NPDs.

Background: The objective of this study was to evaluate treatment outcomes and assess predictors of clinical pregnancy in obstructive azoospermia cases treated with testicular sperm extraction (TESE) and intracytoplasmic sperm injection (ICSI) in Ghana. Methods: This study was a retrospective study conducted on 67 men seeking treatment for obstructive azoospermia at two study sites in Ghana from January 2018 to December 2019. First, archived data were reviewed and treatment outcomes of cases of obstructive azoospermia from the hospital records were evaluated. Infertile men who met the inclusion criteria were recruited. Descriptive data were expressed in the form of frequencies and percentages. The dependent and independent variables were analyzed using multiple logistic regression and reported as odds ratios (ORs). The confidence interval (CI) was set at 95% and a p-value

Additional file 1: Table S1. NMR integral data used to calculate conversion. Table S2. MFI values of different controls in flow cytometry. Figure S1. Characterization of CEAm monomer by 1H NMR. Figure S2. Synthesis and characterization of P(NAM72-b-CEAm74) (PNC) via RAFT polymerization. Figure S3. Characterization of PNAM and PCEAm homopolymers used as controls. Figure S4. Synthesis and characterization of PNCDY-635. Figure S5. Titration of PCEAm and PNC. Figure S6. CLSM study of microparticles shielded with PNDDY635 Polymer. Figure S7. DNA release behavior of naked and shielded PBMD particles at pH 7.4 to pH 5 measured via the heparin release assay (HRA). Figure S8. DLS hydrodynamic diameter distributions and exponential decays from naked PBMD(pDNA) particles and with addition of PNC, PNAM, PCEAm. Figure S9. DLS and cryo-TEM measurements of PBMD and PBMD + PNC (L/C 0.6) at pH 7.4 and pH 5. Figure S10. Uptake of naked and shielded PBMD(pDNA) particles in HEK293T and K-562 cells. Figure S11. Gating strategy for uptake experiments exemplary shown for HEK293T cells. Figure S12. Cytotoxicity of naked and PNC shielded particles in HEK293T and K-562 cells determined by propidium iodide (PI) staining. Figure S13. Mean fluorescence intensity of HEK293T and K-562 cells after transfection with PBMD and PBMD + PNC (L/C 0.6) measured via flow cytometry. Figure S14. Gating strategy for transfection experiments exemplary shown for HEK293T cells. Figure S15. In vivo transfection with pDNA or pDNA encapsulated by PNC shielded particles. Figure S16. DLS hydrodynamic diameter distributions and exponential decays from high concentrated PBMD particles before and after addition of PNC dissolved in buffer with different pH values (pH 7.0 and 7.5). Figure S17. DLS hydrodynamic diameter distributions and exponential decays from high concentrated PBMD particles before and after addition of PNC dissolved in buffer with different pH values (pH 7.2 and 7.3). Figure S18. DLS hydrodynamic diameter distributions and exponential decays from high concentrated PBMD particles before and after addition of PNC dissolved in buffer with different pH values (pH 7.3 and 7.4).

Background The role of neuromuscular blocking agents (NMBAs) in coronavirus disease 2019 (COVID-19) acute respiratory distress syndrome (ARDS) is not fully elucidated. Therefore, we aimed to investigate in COVID-19 patients with moderate-to-severe ARDS the impact of early use of NMBAs on 90-day mortality, through propensity score (PS) matching analysis. Methods We analyzed a convenience sample of patients with COVID-19 and moderate-to-severe ARDS, admitted to 244 intensive care units within the COVID-19 Critical Care Consortium, from February 1, 2020, through October 31, 2021. Patients undergoing at least 2 days and up to 3 consecutive days of NMBAs (NMBA treatment), within 48 h from commencement of IMV were compared with subjects who did not receive NMBAs or only upon commencement of IMV (control). The primary objective in the PS-matched cohort was comparison between groups in 90-day in-hospital mortality, assessed through Cox proportional hazard modeling. Secondary objectives were comparisons in the numbers of ventilator-free days (VFD) between day 1 and day 28 and between day 1 and 90 through competing risk regression. Results Data from 1953 patients were included. After propensity score matching, 210 cases from each group were well matched. In the PS-matched cohort, mean (± SD) age was 60.3 ± 13.2 years and 296 (70.5%) were male and the most common comorbidities were hypertension (56.9%), obesity (41.1%), and diabetes (30.0%). The unadjusted hazard ratio (HR) for death at 90 days in the NMBA treatment vs control group was 1.12 (95% CI 0.79, 1.59, p = 0.534). After adjustment for smoking habit and critical therapeutic covariates, the HR was 1.07 (95% CI 0.72, 1.61, p = 0.729). At 28 days, VFD were 16 (IQR 0–25) and 25 (IQR 7–26) in the NMBA treatment and control groups, respectively (sub-hazard ratio 0.82, 95% CI 0.67, 1.00, p = 0.055). At 90 days, VFD were 77 (IQR 0–87) and 87 (IQR 0–88) (sub-hazard ratio 0.86 (95% CI 0.69, 1.07; p = 0.177). Conclusions In patients with COVID-19 and moderate-to-severe ARDS, short course of NMBA treatment, applied early, did not significantly improve 90-day mortality and VFD. In the absence of definitive data from clinical trials, NMBAs should be indicated cautiously in this setting.

AIMS: The clinical effectiveness of primary prevention implantable cardioverter defibrillator (ICD) therapy is under debate. The EUropean Comparative Effectiveness Research to Assess the Use of Primary ProphylacTic Implantable Cardioverter Defibrillators (EU-CERT-ICD) aims to assess its current clinical value. METHODS AND RESULTS: The EU-CERT-ICD is a prospective investigator-initiated non-randomized, controlled, multicentre observational cohort study performed in 44 centres across 15 European Union countries. We will recruit 2250 patients with ischaemic or dilated cardiomyopathy and a guideline indication for primary prophylactic ICD implantation. This sample will include 1500 patients at their first ICD implantation and 750 patients who did not receive a primary prevention ICD despite having an indication for it (non-randomized control group). The primary endpoint is all-cause mortality; the co-primary endpoint in ICD patients is time to first appropriate shock. Secondary endpoints include sudden cardiac death, first inappropriate shock, any ICD shock, arrhythmogenic syncope, revision procedures, quality of life, and cost-effectiveness. At baseline (and prior to ICD implantation if applicable), all patients undergo 12-lead electrocardiogram (ECG) and Holter ECG analysis using multiple advanced methods for risk stratification as well as detailed documentation of clinical characteristics and laboratory values. Genetic biobanking is also organized. As of August 2018, baseline data of 2265 patients are complete. All subjects will be followed for up to 4.5 years. CONCLUSIONS: The EU-CERT-ICD study will provide a necessary update about clinical effectiveness of primary prophylactic ICD implantation. This study also aims for improved risk stratification and patient selection using clinical and ECG risk markers. ispartof: ESC HEART FAILURE vol:6 issue:1 pages:182-193 ispartof: location:England status: published

More than a hundred distinct modified nucleosides have been identified in RNA, but little is known about their distribution across different organisms, dynamic nature and their response to cellular and environmental stress. Mass spectrometry based methods have been at the forefront of identifying and quantifying modified nucleosides. However, they often require synthetic reference standards, which do not exist for many modified nucleosides and therefore impedes their analysis. Here, we use a metabolic labeling approach to rapidly generate bio-isotopologues of the complete C. elegans transcriptome and its modifications, and use them as reference standards to characterize the RNA modification profile in this multicellular organism via an untargeted liquid-chromatography tandem high-resolution mass spectrometry (LC-MS/HRMS) approach. We furthermore show that several of these RNA modifications have a dynamic response to environmental stress and that in particular changes in the tRNA wobble base modification 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) lead to codon biased gene expression changes in starved animals., CRUK Wellcome Trust

The F(-), Cl(-), and Br(-) binding selectivity of bis(p-nitroanilide)s of dipicolinic and isophthalic acids was studied by using competitive electrospray mass spectrometry and UV-Visible spectroscopy. Both hosts prefer binding Cl(-) over either F(-) or Br(-). Host deprotonation was observed to some extent in all experiments in which the host was exposed to halide ions. When F(-) was present, host deprotonation was often the major process, whereas little deprotonation was observed by Cl(-) or Br(-), which preferred complexation. A solution of either host changed color when mixed with a F(-), H(2)PO(4)(-), di- or triphenylacetate solution.

Metamodeling addresses the issue of formally defining the vocabulary and rules sub- sequently used in modeling activities. On one hand, metamodeling is often identified as a key component in information system development. It defines in a formal way the modeling prim- itives that will be used in system analysis and design activites. On other hand, conceptual graphs are often identified as a key language in knowledge representation because they are sim- ple, expressive and closelyrelated to other familiar modelling languages such as UML or E/R. We propose in this paper a metamodel for conceptual graphs.

OBJECTIVE: Aggregation of α-synuclein (α-syn) and α-syn cytotoxicity are hallmarks of sporadic and familial Parkinson disease (PD), with accumulating evidence that prefibrillar oligomers and protofibrils are the pathogenic species in PD and related synucleinopathies. Peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), a key regulator of mitochondrial biogenesis and cellular energy metabolism, has recently been associated with the pathophysiology of PD. Despite extensive effort on studying the function of PGC-1α in mitochondria, no studies have addressed whether PGC-1α directly influences oligomerization of α-syn or whether α-syn oligomers impact PGC-1α expression. MATERIALS AND METHODS: We tested whether pharmacological or genetic activation of PGC-1α or PGC-11α knockdown could modulate the oligomerization of α-syn in vitro by using an α-syn -fragment complementation assay. RESULTS: In this study, we found that both PGC-1α reference gene (RG-PGC-1α) and the central nervous system (CNS)-specific PGC-1α (CNS-PGC-1α) are downregulated in human PD brain, in A30P α-syn transgenic animals, and in a cell culture model for α-syn oligomerization. Importantly, downregulation of both RG-PGC-1α and CNS-PGC-1α in cell culture or neurons from RG-PGC-1α-deficient mice leads to a strong induction of α-syn oligomerization and toxicity. In contrast, pharmacological activation or genetic overexpression of RG-PGC-1α reduced α-syn oligomerization and rescued α-syn-mediated toxicity. INTERPRETATION: Based on our results, we propose that PGC-1α downregulation and α-syn oligomerization form a vicious circle, thereby influencing and/or potentiating each other. Our data indicate that restoration of PGC-1α is a promising approach for development of effective drugs for the treatment of PD and related synucleinopathies. ispartof: Annals of Neurology vol:77 issue:1 pages:15-32 ispartof: location:United States status: published

Aggregation of α-synuclein (α-syn) and α-syn cytotoxicity are hallmarks of sporadic and familial Parkinson disease (PD), with accumulating evidence that prefibrillar oligomers and protofibrils are the pathogenic species in PD and related synucleinopathies. Peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), a key regulator of mitochondrial biogenesis and cellular energy metabolism, has recently been associated with the pathophysiology of PD. Despite extensive effort on studying the function of PGC-1α in mitochondria, no studies have addressed whether PGC-1α directly influences oligomerization of α-syn or whether α-syn oligomers impact PGC-1α expression.We tested whether pharmacological or genetic activation of PGC-1α or PGC-11α knockdown could modulate the oligomerization of α-syn in vitro by using an α-syn -fragment complementation assay.In this study, we found that both PGC-1α reference gene (RG-PGC-1α) and the central nervous system (CNS)-specific PGC-1α (CNS-PGC-1α) are downregulated in human PD brain, in A30P α-syn transgenic animals, and in a cell culture model for α-syn oligomerization. Importantly, downregulation of both RG-PGC-1α and CNS-PGC-1α in cell culture or neurons from RG-PGC-1α-deficient mice leads to a strong induction of α-syn oligomerization and toxicity. In contrast, pharmacological activation or genetic overexpression of RG-PGC-1α reduced α-syn oligomerization and rescued α-syn-mediated toxicity.Based on our results, we propose that PGC-1α downregulation and α-syn oligomerization form a vicious circle, thereby influencing and/or potentiating each other. Our data indicate that restoration of PGC-1α is a promising approach for development of effective drugs for the treatment of PD and related synucleinopathies.

The synthesis and in vitro and in vivo evaluation of 12,13-cycloalkyl-6,11-ethanobenzo[b]-quinolizidines, a new class of noncompetitive N-methyl-D-aspartate (NMDA) antagonists acting at the PCP site on the NMDA receptor complex, is reported. Structure-activity relationship studies led to the identification of 10-hydroxy-(6 alpha,11 alpha,11 alpha beta,12R*,13S*)-1,3,4,6,11,11a,13,14,15,16-decahydro-12H- 6,11[1',2']-endo-cyclopenta-2H-pyrido[1,2-b]isoquinoline hydrobromide (5h) and 9-hydroxy-(6 alpha,11 alpha,11a beta,12R*,13S*)- 1,3,4,6,11,11a,13,14,15,16-decahydro-12H-6,11-[1',2']-endo-cycl ope nta-2H- pyrido[1,2-b]isoquinoline hydrobromide (5i), the most potent members of this series with Ki values of 2.3 +/- 0.2 and 2.3 +/- 0.5 nM, respectively. Molecular modeling studies revealed that this series of compounds occupies both lipophilic sites of the Andrews PCP receptor model and shares structural features which are common to other classes of known noncompetitive NMDA antagonists such as MK-801.

Detailed conformational analysis of 1-phenyl-1H-4,5-dihydro-2,4-benzodiazepines using X-ray structures, nuclear Overhauser experiments, variable-temperature 1 H NMR, and MacroModel (MULTIC) reveals a rapid equilibrium between axial and equatorial comformations for the free base of 2. The DCl salt of 2 and 1-methyl-1-phenyl analog 3, however, prefer the axial conformation

With the aim of testing the fluorine atom as a potential cation-stabilizing (C-S) auxiliary for enhancing polyene cyclizations, new methodology has been developed for the stereoselective synthesis of polyenic cyclization substrates in which the vinyl H of an E-trisubstituted olefinic bond is replaced by F

The use of the fluorine atom an a cation-stabilizing (C-S) auxiliary for enhancing polyene cyclization has been improved in synthetic effectiveness by the demonstration that an acetal 5 can be converted to a racemic fluorocycle, bearing the natural backbone configuration of the steroids

The fluorine atom substituted at the pro-C-13 position (steroid numbering) has been shown to be an effective cation-stabilizing (C-S) auxiliary in the acid-catalyzed cyclization of polyene substrates. The preparation of these polyenes employed the known fragmentation of cyclic epoxy hydrazones to construct the methylacetylenic and gem-dimethyl groups. The fluoroolefinic ketal Claisen and cyclopropylcarbinol rearrangements established the 3(E) and 11 (E) trisubstituted alkenes

A series of novel isoxazolo analogues 11 of the dibenzazonine alkaloid protostephanine (1) has been prepared. A new regiospecific and potentially general aza macrocyclic ring forming process was developed where the first step was the 1,3-dipolar cycloaddition of benzonitrile oxide to the readily available examine 6. The product, isoxazoline 9a, under solvolytic conditions that normally convert monocyclic 5-aminoisoxazolines to isoxazoles failed to give the desired aza macrocycle 14. Alternate sequences involving quaternization of 9a with methyl iodide followed by either solvolysis in polar solvents or base-induced elimination were successful and gave the target structure 11a in excellent overall yield. X-ray crystallographic analysis of the quaternized intermediate 10a corroborated the assignment of structure and defined the crystal-state conformation

A series of analogues of the 5-lipoxygenase inhibitor 1-phenyl-3-pyrazolidinone (phenidone, 1a) has been prepared via two complementary new synthetic methods. The reaction of various electgrophiles with the dianion of 1a or with an N-silylpyrazolidinone anion gave the desired 4-substituted pyrazolidinones (Scheme I and II). A new procedure was developed for the resolution of 4-substitued pyrazolidinones (Scheme V). A regression study on 21 compounds in this series showed a correlation of increased inhibitor potency (pIC 50 ) with increased compound lipophilicity (log P) and with an N-phenyl electronic effect as measured by the 13 C NMR chemical shift parameter CNMR1' (R 2 =0.79). The most potent 5-lipoxygenase inhibitor in this series was 4-(ethylthio)-1-phenyl-3-pyrazolidinone (1n) with an IC 50 of 60 nM. Another member of this series, 4-(2-methoxyethyl)-1-phenyl-3-pyrazolidinone (1f, IC 50 =0.48 μM), although less potent than 1n, was betgter tolerated in the whole animal relative to phenidone (1a) and also displayed good oral activity in two models of 5-lipoxygenase inhibition. On the basis of a structure-activity relationship study, a mechanism for the inhibition of 5-lipoxygenase by this class of inhibitors was proposed

Objective: Elderly patients are not included in most clinical trails. Therefore, evidence-based data on how to treat those patients are scarce. However, the growing number of elderly patients brings that problem into the focus. We performed that study to investigate whether elderly (>75 years) [for full text, please go to the a.m. URL], 61. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC) im Rahmen der Neurowoche 2010

The thermal decomposition of the 1:1 adducts of ethylenediamine (en) with the trialkylaluminum compounds R 3 Al (R=Me, Et) in aromatic hydrocarbon solvents leads to the formation of insoluble polymeric solids whose composition corresponds approximately to the formula [RAl(en-2H)] n . These thermolysis reactions proceed through a series of intermediates which were identified with the aid of 1 H, 13 C, and 27 Al NMR spectroscopy.

Part one. The NMR spectra of six-membered ring compounds show an approximately three times larger spin coupling constant for 1,2-diaxial hydrogen atoms than for 1,2-hydrogen atoms in other conformational relationship. Signals for equatorial hydrogen atoms are generally found at lower fields than those for the corresponding axial hydrogen atoms. The signals of equatorial acetoxy or methoxy groups, however, are usually observed at higher fields than their axial equivalent. The observations are applied to several determinations of configuration and conformation with emphasis on the acetylated aldopyranoses and some theoretical implications of the results are discussed. Part two. It was found possible to assign the structures and configurations to two of the diastereoisomeric 1,3-dimethoxy-2-acetoxycyclohexanes on the basis of their NMR spectra and thereby assign configurations to the parent diastereoisomeric 3-methoxycyclohexene oxides. The conclusions thus reached were substantiated by chemical means. Two new isomeric 3-methoxy-1,2-cyclohexanediols were isolated and their structures were established. The rates of the periodate oxidation of these compounds and of the other two previously known isomers were examined. Furthermore, the relative electrophoretic mobilities of the four isomers, on borate buffered filter paper, were determined. The structures and configurations of the epimeric 3-methoxy derivatives of trans-1-tosyloxy-2-acetoxycyclohexane were established and the rates of their acetolyses measured. The results of the rate studies and electrophoretic migration measurements are discussed in terms of interactions between the 3-methoxy groups and the 1,2-cyclic ions formed as reaction intermediates.

The reactions of triethylaluminum and trimethylaluminum with ethylenediamine have been studied. Heating of the initially formed (R 3 Al) 2 •en and R 3 Al•en adduct mixture results in the formation of RAl[(HN(CH 2 ) 2 NH)AlR 2 ] 2 (R=CH 3 , and C 2 H 5 ). The structures and formation mechanisms of these two compounds, as well as their pyrolysis reactions, have been studied with 1 H, 13 C, and 27 Al NMR, FTIR, MS, GC, DSC, and TGA techniques. The methyl derivative was characterized by a single-crystal X-ray diffraction analysis: space group P2/c

With the Global War on Terror (GWOT) beginning its sixth year and insurgencies evident in various regions across the globe, the underlying question is: are we fighting one global insurgency or isolated insurgent and/or terrorist organizations? The purpose of this paper is to explore the answer to that question. It reviews a sampling of terrorist/insurgency organizations operating worldwide and explores their ideologies and methodologies. Once this baseline is established, the argument is made that Al Qaeda is an insurgency with a global reach. However, the current world situation mandates a broader view than the myopic focus currently being given to a potential radical Islamic jihad.

Aging is associated with musculoskeletal changes and altered walking patterns. These changes are common in people with knee osteoarthritis (OA) and may precipitate the development of OA. We examined age-related changes in musculoskeletal structures and walking patterns to better understand the relationship between aging and knee OA.

We report on the first radioactive beam experiment performed at the recently commissioned REX-ISOLDE facility at CERN in conjunction with the highly efficient $\gamma$ -spectrometer MINIBALL. Using $^{30}$Mg ions accelerated to an energy of 2.25MeV/u together with a thin $^{nat}$Ni target, Coulomb excitation of the first excited 2+ states of the projectile and target nuclei well below the Coulomb barrier was observed. From the measured relative de-excitation $\gamma$ -ray yields the B(E2; 0$^{+}_{gs} \rightarrow 2^{+}_{1}$) value of $^{30}$Mg was determined to be 241(31)$e^{2}$fm$^{4}$. Our result is lower than values obtained at projectile fragmenttion facilities using the intermediate-energy Coulomb excitation method and confirms that the theoretical conjecture that the neutron-rich magnesium isotope $^{30}$Mg lies still outside the "island of inversion". We report on the first radioactive beam experiment performed at the recently commissioned REX-ISOLDE facility at CERN in conjunction with the highly efficient gamma spectrometer MINIBALL. Using 30Mg ions accelerated to an energy of 2.25 MeV/u together with a thin nat-Ni target, Coulomb excitation of the first excited 2+ states of the projectile and target nuclei well below the Coulomb barrier was observed. From the measured relative de-excitation gamma ray yields the B(E2: 0+ -> 2+) value of 30Mg was determined to be 241(31) e2fm4. Our result is lower than values obtained at projectile fragmentation facilities using the intermediate-energy Coulomb excitation method, and confirms the theoretical conjecture that the neutron-rich magnesium isotope 30Mg lies still outside the ``island of inversion''.

Fluorine-assisted asymmetric pentacyclization of (S,S)-acetal 3 gave 18α(H)-oleanane 28b as the major product in 59% isolated yield (86.5% de) and with retention of the fluorine atom at C13. © 1994, American Chemical Society. All rights reserved.

The Radioactive Beam Experiment {REX-ISOLDE} [1,2] is a pilot experiment at ISOLDE (CERN) testing the new concept of post acceleration of radioactive ion beams by using charge breeding of the ions in a high charge state ion source and the efficient acceleration of the highly charged ions in a short LINAC using modern ion accelerator structures. In order to prepare the ions for the experiments singly charged radioactive ions from the on-line mass separator ISOLDE will be cooled and bunched in a Penning trap, charge bred in an electron beam ion source (EBIS) and finally accelerated in the LINAC. The LINAC consists of a radio frequency quadrupole (RFQ) accelerator, which accelerates the ions up to 0.3 MeV/u, an 20 gap interdigital H-type (IH) structure with a final energy between 1.1 and 1.2 MeV/u and three seven gap resonators, which allow the variation of the final energy between 0.8-2.2 MeV/u. A variety of tests with REXTRAP, REXEBIS and the LINAC structures have been done, in order to study their capabilities. An emittance meter for beam intensities down to 0.5 nA and a test beam line for the front part of the REX-ISOLDE LINAC have been built up in order to measure beam emittances of the different components of REX-ISOLDE and beam energy, energy spread and transmission of the LINAC structures. The results of these measurements and the first tests at CERN will be presented.

The radioactive beam experiment REX-ISOLDE, a pilot experiment testing a new concept of post acceleration of radioactive ions at ISOLDE/CERN is in progress. Singly charged radioactive ions delivered by the online mass separator ISOLDE are accumulated in a Penning trap (REX trap), charge bred in an electron beam ion source (EBIS), separated from the residual gas in a mass separator and then accelerated in a linac with output energies between 0.8 and 2.2 MeV /u. The REX trap is in operation, a first test beam was already injected. The design phase of the EBIS is finished and the construction has been started. The superconducting magnet is delivered. The linac consists of a radiofrequency quadrupole (RFQ) accelerator, an interdigital IH-structure and 3 seven gap resonators to vary the final energy. (12 refs).