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The world is going green; hence, environmentally friendly practices that would conserve natural resources for the future generation are encouraged. As a consequence, the world is less concerned about the numerous applications of nanotechnology, especially in the health sector; rather, it is more concerned about the sustainability of functionalized nanomaterials. Thus, the future of nanotechnology depends on its ability to ‘go green'. Green nanotechnology attempts to synthesize improved, nontoxic, and biocompatible nanomaterials with sustainable benefits using eco-friendly materials. Although green nanotechnology is considered a sustainable, viable, and biocompatible approach to the production of eco-friendly nanomaterials, there are shortcomings especially in microbial handling and process optimization. In this chapter, the authors aim to appraise not only the use of biocompatible approaches for the synthesis of nanoparticles and/or nanomaterials but also their shortcomings.