1. Vitamin D supplementation lowers thrombospondin-1 levels and blood pressure in healthy adults
- Author
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Natasha M. Rogers, Aaron L. Sverdlov, B. Assadi-Khansari, Marilyn Black, Greer Dymmott, Saifei Liu, Doan T.M. Ngo, Anjalee T Amarasekera, John D. Horowitz, Amarasekera, Anjalee T, Assadi-Khansari, Bahador, Liu, Saifei, Black, Marilyn, Dymmott, Greer, Rogers, Natasha M, Sverdlov, Aaron L, Horowitz, John D, and Ngo, Doan TM
- Subjects
0301 basic medicine ,Male ,Physiology ,Organic chemistry ,lcsh:Medicine ,Blood Pressure ,030204 cardiovascular system & hematology ,Vascular Medicine ,Biochemistry ,Thrombospondin 1 ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,Fibrosis ,Animal Cells ,South Australia ,Medicine and Health Sciences ,Medicine ,Insulin ,Vitamin D ,Enzyme-Linked Immunoassays ,lcsh:Science ,vitamin D insufficiency ,Multidisciplinary ,Neurochemistry ,Vitamins ,Middle Aged ,Healthy Volunteers ,Body Fluids ,Physical sciences ,Chemistry ,Blood ,Female ,medicine.symptom ,Anatomy ,Neurochemicals ,Cellular Types ,Research Article ,Adult ,Platelets ,medicine.medical_specialty ,Diastole ,inflammation and fibrosis ,Inflammation ,Nitric Oxide ,Research and Analysis Methods ,Blood Plasma ,Nitric oxide ,03 medical and health sciences ,Chemical compounds ,Internal medicine ,Organic compounds ,Vitamin D and neurology ,Humans ,cardio-metabolic dysfunction ,Immunoassays ,Diabetic Endocrinology ,Blood Cells ,business.industry ,lcsh:R ,Biology and Life Sciences ,Cell Biology ,medicine.disease ,Hormones ,030104 developmental biology ,Blood pressure ,chemistry ,Immunologic Techniques ,lcsh:Q ,business ,Asymmetric dimethylarginine ,Plasminogen activator ,Biomarkers ,Neuroscience - Abstract
Introduction Vitamin D insufficiency, defined as 25-hydroxyVitamin D (25(OH)D) levels 75nmol/L is associated with cardio-metabolic dysfunction. Vitamin D insufficiency is associated with inflammation and fibrosis, but it remains uncertain whether these anomalies are readily reversible. Therefore, we aimed to determine the effects of Vitamin D supplementation on markers of: 1) nitric oxide (NO) signaling, 2) inflammation, and 3) fibrosis, in healthy volunteers with mild hypovitaminosis. Methods Healthy volunteers (n = 35) (mean age: 45 ± 11 years) with 25(OH)D levels 75nmol/L, received Vitamin D supplementation (Ostelin capsules 2000IU) for 12 weeks. Resting systolic and diastolic blood pressures (BP) were assessed. Routine biochemistry was examined. Plasma concentrations of asymmetric dimethylarginine (ADMA), thrombospondin-1 (TSP-1), plasminogen activator inhibitor- 1 (PAI-1), hs-CRP, activin-A, and follistatin-like 3 (FSTL3) were quantitated. Results Vitamin D administration for 12 weeks significantly increased 25-(OH)D levels (48.8 ± 16 nmol/L to 100.8 ± 23.7 nmol/L, p0.001). There was significant lowering of systolic and diastolic BP, while there was no significant change in lipid profiles, or fasting insulin. Plasma concentrations of ADMA, hs-CRP, PAI-1, activin A, and FSTL-3 did not change with Vitamin D supplementation. However, there was a marked reduction of TSP-1 (522.7 ± 379.8 ng/mL vs 206.7 ± 204.5 ng/mL, p0.001). Conclusions Vitamin D supplementation in Vitamin D insufficient, but otherwise healthy individuals markedly decreased TSP-1 levels and blood pressure. Since TSP-1 suppresses signaling of NO, it is possible that the fall in BP is engendered by restoration of NO effect. Refereed/Peer-reviewed
- Published
- 2017