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Full list of authors: Doroshenko, Victor; Poutanen, Juri; Tsygankov, Sergey S.; Suleimanov, Valery F.; Bachetti, Matteo; Caiazzo, Ilaria; Costa, Enrico; Di Marco, Alessandro; Heyl, Jeremy; La Monaca, Fabio; Muleri, Fabio; Mushtukov, Alexander A.; Pavlov, George G.; Ramsey, Brian D.; Rankin, John; Santangelo, Andrea; Soffitta, Paolo; Staubert, Rüdiger; Weisskopf, Martin C.; Zane, Silvia; Agudo, Iván; Antonelli, Lucio A.; Baldini, Luca; Baumgartner, Wayne H.; Bellazzini, Ronaldo; Bianchi, Stefano; Bongiorno, Stephen D.; Bonino, Raffaella; Brez, Alessandro; Bucciantini, Niccolò; Capitanio, Fiamma; Castellano, Simone; Cavazzuti, Elisabetta; Ciprini, Stefano; De Rosa, Alessandra; Del Monte, Ettore; Di Gesu, Laura; Di Lalla, Niccolò; Donnarumma, Immacolata; Dovčiak, Michal; Ehlert, Steven R.; Enoto, Teruaki; Evangelista, Yuri; Fabiani, Sergio; Ferrazzoli, Riccardo; Garcia, Javier A.; Gunji, Shuichi; Hayashida, Kiyoshi; Iwakiri, Wataru; Jorstad, Svetlana G.; Karas, Vladimir; Kitaguchi, Takao; Kolodziejczak, Jeffery J.; Krawczynski, Henric; Latronico, Luca; Liodakis, Ioannis; Maldera, Simone; Manfreda, Alberto; Marin, Frédéric; Marinucci, Andrea; Marscher, Alan P.; Marshall, Herman L.; Matt, Giorgio; Mitsuishi, Ikuyuki; Mizuno, Tsunefumi; Ng, Chi-Yung; O'Dell, Stephen L.; Omodei, Nicola; Oppedisano, Chiara; Papitto, Alessandro; Peirson, Abel L.; Perri, Matteo; Pesce-Rollins, Melissa; Pilia, Maura; Possenti, Andrea; Puccetti, Simonetta; Ratheesh, Ajay; Romani, Roger W.; Sgrò, Carmelo; Slane, Patrick; Spandre, Gloria; Sunyaev, Rashid A.; Tamagawa, Toru; Tavecchio, Fabrizio; Taverna, Roberto; Tawara, Yuzuru; Tennant, Allyn F.; Thomas, Nicolas E.; Tombesi, Francesco; Trois, Alessio; Turolla, Roberto; Vink, Jacco; Wu, Kinwah; Xie, Fei., Using observations of X-ray pulsar Hercules X-1 by the Imaging X-ray Polarimetry Explorer we report a highly significant (>17σ) detection of the polarization signal from an accreting neutron star. The observed degree of linear polarization of ~10% is far below theoretical expectations for this object, and stays low throughout the spin cycle of the pulsar. Both the degree and angle of polarization exhibit variability with the pulse phase, allowing us to measure the pulsar spin position angle 57(2) deg and the magnetic obliquity 12(4) deg, which is an essential step towards detailed modelling of the intrinsic emission of X-ray pulsars. Combining our results with the optical polarimetric data, we find that the spin axis of the neutron star and the angular momentum of the binary orbit are misaligned by at least ~20 deg, which is a strong argument in support of the models explaining the stability of the observed superorbital variability with the precession of the neutron star. © 2022, The Author(s), under exclusive licence to Springer Nature Limited., This Article is based on observations made by IXPE, a joint US and Italian mission. This research used data products provided by the IXPE Team (MSFC, SSDC, INAF and INFN) and distributed with additional software tools by the High-Energy Astrophysics Science Archive Research Center (HEASARC), at the NASA Goddard Space Flight Center (GSFC). The US contribution is supported by NASA and led and managed by its Marshall Space Flight Center (MSFC), with industry partner Ball Aerospace (contract number NNM15AA18C). The Italian contribution is supported by the Italian Space Agency (Agenzia Spaziale Italiana, ASI) through contract number ASI-OHBI-2017-12-I.0, agreement numbers ASI-INAF-2017-12-H0 and ASI-INFN-2017.13-H0, and its Space Science Data Center (SSDC) and by the Istituto Nazionale di Astrofisica (INAF) and the Istituto Nazionale di Fisica Nucleare (INFN) in Italy. V.D. and V.F.S. acknowledge support from the German Academic Exchange Service (DAAD) under travel grant number 57525212. J.P. and S.S.T. thank the Russian Science Foundation for support under grant number 20-12-00364 and the Academy of Finland for support under grant numbers 333112, 349144, 349373 and 349906. V.F.S. thanks the German Research Foundation (DFG) for grant number WE 1312/53-1. I.C. is a Sherman Fairchild Fellow at Caltech and thanks the Burke Institute at Caltech for supporting her research. A.A.M. acknowledges support from the Netherlands Organization for Scientific Research Veni Fellowship., info:eu-repo/grantAgreement/MICINN//SEV-2017-0709

Purpose: Teachers are occupational voice users with significant vocal demand. This study examined if a vocal hygiene program could mitigate the effects of occupational vocal demand in primary school teachers across 1 month. Method: Sixty female teachers participated, with 30 in an experimental group receiving vocal hygiene education plus daily home practice for 1 month and 30 in a control group with no intervention. Their vocal changes across the month were quantified with (a) acoustic measures on fundamental frequency ( f o ), vocal intensity, jitter and shimmer, harmonics-to-noise ratio, and smoothed cepstral peak prominence and (b) Voice Handicap Index (VHI-10) and Vocal Fatigue Index (VFI) scores. Results: Analysis of covariance showed significantly larger changes (significant decreases) in conversational f o and in jitter for the experimental group relative to the control group. Post hoc pairwise comparisons following repeated-measures analysis of variance showed significant decreases in conversational f o and in jitter across the month for the experimental group. No significant differences in VHI-10 and VFI scores were found between the groups. Conclusions: Vocal demand–related changes in acoustic measures could be partially mitigated with the vocal hygiene program. Future studies with a more refined intervention program and more long-term follow-up are recommended to better understand the long-term benefits of vocal hygiene programs on primary school teachers.

This paper discusses the academic preparation university students receive in Japan before going abroad, and to what extent it sets them up for success in foreign learning environments. The hypothesis is that not preparing students adequately in English for Academic Purposes (EAP), even high proficiency English language learners, could leave them overwhelmed when they join overseas study programmes. In order to test the hypothesis, semi-structured interviews were conducted with a group of Japanese students studying at a UK university. The same students were interviewed a second time, after a gap of seven months, to note any changes in their perception of their study experience. Preliminary findings indicate that EAP is not fully understood or supported in Japan and that changes are required in EAP instruction and institutional support for it.

Full list of authors: Liodakis, Ioannis; Marscher, Alan P.; Agudo, Ivan; Berdyugin, Andrei V.; Bernardos, Maria I.; Bonnoli, Giacomo; Borman, George A.; Casadio, Carolina; Casanova, Victor; Cavazzuti, Elisabetta; Cavero, Nicole Rodriguez; Di Gesu, Laura; Di Lalla, Niccolo; Donnarumma, Immacolata; Ehlert, Steven R.; Errando, Manel; Escudero, Juan; Garcia-Comas, Maya; Agis-Gonzalez, Beatriz; Husillos, Cesar; Jormanainen, Jenni; Jorstad, Svetlana G.; Kagitani, Masato; Kopatskaya, Evgenia N.; Kravtsov, Vadim; Krawczynski, Henric; Lindfors, Elina; Larionova, Elena G.; Madejski, Grzegorz M.; Marin, Frederic; Marchini, Alessandro; Marshall, Herman L.; Morozova, Daria A.; Massaro, Francesco; Masiero, Joseph R.; Mawet, Dimitri; Middei, Riccardo; Millar-Blanchaer, Maxwell A.; Myserlis, Ioannis; Negro, Michela; Nilsson, Kari; O'Dell, Stephen L.; Omodei, Nicola; Pacciani, Luigi; Paggi, Alessandro; Panopoulou, Georgia V.; Peirson, Abel L.; Perri, Matteo; Petrucci, Pierre-Olivier; Poutanen, Juri; Puccetti, Simonetta; Romani, Roger W.; Sakanoi, Takeshi; Savchenko, Sergey S.; Sota, Alfredo; Tavecchio, Fabrizio; Tinyanont, Samaporn; Vasilyev, Andrey A.; Weaver, Zachary R.; Zhovtan, Alexey V.; Antonelli, Lucio A.; Bachetti, Matteo; Baldini, Luca; Baumgartner, Wayne H.; Bellazzini, Ronaldo; Bianchi, Stefano; Bongiorno, Stephen D.; Bonino, Raffaella; Brez, Alessandro; Bucciantini, Niccolo; Capitanio, Fiamma; Castellano, Simone; Ciprini, Stefano; Costa, Enrico; De Rosa, Alessandra; Del Monte, Ettore; Di Marco, Alessandro; Doroshenko, Victor; Dovciak, Michal; Enoto, Teruaki; Evangelista, Yuri; Fabiani, Sergio; Ferrazzoli, Riccardo; Garcia, Javier A.; Gunji, Shuichi; Hayashida, Kiyoshi; Heyl, Jeremy; Iwakiri, Wataru; Karas, Vladimir; Kitaguchi, Takao; Kolodziejczak, Jeffery J.; La Monaca, Fabio; Latronico, Luca; Maldera, Simone; Manfreda, Alberto; Marinucci, Andrea; Matt, Giorgio; Mitsuishi, Ikuyuki; Mizuno, Tsunefumi; Muleri, Fabio; Ng, Stephen C. -Y.; Oppedisano, Chiara; Papitto, Alessandro; Pavlov, George G.; Pesce-Rollins, Melissa; Pilia, Maura; Possenti, Andrea; Ramsey, Brian D.; Rankin, John; Ratheesh, Ajay; Sgro, Carmelo; Slane, Patrick; Soffitta, Paolo; Spandre, Gloria; Tamagawa, Toru; Taverna, Roberto; Tawara, Yuzuru; Tennant, Allyn F.; Thomas, Nicolas E.; Tombesi, Francesco; Trois, Alessio; Tsygankov, Sergey; Turolla, Roberto; Vink, Jacco; Weisskopf, Martin C.; Wu, Kinwah; Xie, Fei; Zane, Silvia.--This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited., Most of the light from blazars, active galactic nuclei with jets of magnetized plasma that point nearly along the line of sight, is produced by high-energy particles, up to around 1 TeV. Although the jets are known to be ultimately powered by a supermassive black hole, how the particles are accelerated to such high energies has been an unanswered question. The process must be related to the magnetic field, which can be probed by observations of the polarization of light from the jets. Measurements of the radio to optical polarization—the only range available until now—probe extended regions of the jet containing particles that left the acceleration site days to years earlier1,2,3, and hence do not directly explore the acceleration mechanism, as could X-ray measurements. Here we report the detection of X-ray polarization from the blazar Markarian 501 (Mrk 501). We measure an X-ray linear polarization degree ΠX of around 10%, which is a factor of around 2 higher than the value at optical wavelengths, with a polarization angle parallel to the radio jet. This points to a shock front as the source of particle acceleration and also implies that the plasma becomes increasingly turbulent with distance from the shock. © The Author(s) 2022., I.L. was supported by the JSPS postdoctoral short-term fellowship programme. The Imaging X-ray Polarimetry Explorer (IXPE) is a joint US and Italian mission. The US contribution is supported by the National Aeronautics and Space Administration (NASA) and led and managed by its Marshall Space Flight Center (MSFC), with industry partner Ball Aerospace (contract NNM15AA18C). The Italian contribution is supported by the Italian Space Agency (Agenzia Spaziale Italiana, ASI) through contract ASI-OHBI-2017-12-I.0, agreements ASI-INAF-2017-12-H0 and ASI-INFN-2017.13-H0, and its Space Science Data Center (SSDC) with agreements ASI-INAF-2022-14-HH.0 and ASI-INFN 2021-43-HH.0, and by the Istituto Nazionale di Astrofisica (INAF) and the Istituto Nazionale di Fisica Nucleare (INFN) in Italy. This research used data products provided by the IXPE Team (MSFC, SSDC, INAF and INFN) and distributed with additional software tools by the High-Energy Astrophysics Science Archive Research Center (HEASARC), at NASA Goddard Space Flight Center (GSFC). Data from the Steward Observatory spectropolarimetric monitoring project were used. This programme is supported by Fermi Guest Investigator grants NNX08AW56G, NNX09AU10G, NNX12AO93G and NNX15AU81G. This research has made use of data from the RoboPol programme, a collaboration between Caltech, the University of Crete, the Institute of Astrophysics-Foundation for Research and Technology (IA-FORTH), the Inter-University Centre for Astronomy and Astrophysics (IUCAA), the Max Planck Institute for Radioastronomy (MPIfR) and the Nicolaus Copernicus University, which was conducted at Skinakas Observatory in Crete, Greece. The Instituto Astrofísica Andalucía (IAA)-Consejo Superior de Investigaciones Científicas (CSIC) co-authors acknowledge financial support from the Spanish Ministerio de Ciencia e Innovacion (MCINN) through the ‘Center of Excellence Severo Ochoa‘ award for the Instituto de Astrofisica de Andalucia-CSIC (SEV-2017-0709). Acquisition and reduction of the POLAMI and Monitoring AGN with Polarimetry at the Calar Alto Telescopes (MAPCAT) data were supported in part by Ministerio de Ciencia e Innovación (MICINN) through grants AYA2016-80889-P and PID2019-107847RB-C44. The POLAMI observations were carried out at the IRAM 30 m Telescope. IRAM is supported by the National Institute of Sciences of the Universe (INSU)/Scientific Research National Center (CNRS) (France), Max-Planck-Gesellschaft (MPG) (Germany) and Instituto Geográfico Nacional (IGN) (Spain). The research at Boston University was supported in part by National Science Foundation grant AST-2108622, NASA Fermi Guest Investigator grant 80NSSC21K1917 and NASA Swift Guest Investigator grant 80NSSC22K0537. This study uses observations conducted with the 1.8 m Perkins Telescope Observatory in Arizona (USA), which is owned and operated by Boston University. Based on observations obtained at the Hale Telescope, Palomar Observatory as part of a continuing collaboration between the California Institute of Technology, NASA/Jet Propulsion Laboratory (JPL), Yale University and the National Astronomical Observatories of China. This research made use of Photutils, an Astropy package for detection and photometry of astronomical sources60. G.V.P. acknowledges support by NASA through the NASA Hubble Fellowship grant no. HST-HF2-51444.001-A awarded by the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Incorporated, under NASA contract NAS5-26555. Based on observations made with the Nordic Optical Telescope, owned in collaboration by the University of Turku and Aarhus University, and operated jointly by Aarhus University, the University of Turku and the University of Oslo, representing Denmark, Finland and Norway, the University of Iceland and Stockholm University at the Observatorio del Roque de los Muchachos, La Palma, Spain, of the Instituto de Astrofisica de Canarias. The data presented here were obtained (in part) with ALFOSC, which is provided by the Instituto de Astrofisica de Andalucia (IAA) under a joint agreement with the University of Copenhagen and the Nordic Optical Telescope. V.K. thanks the Vilho, Yrjö and Kalle Väisälä Foundation. J.J. was supported by Academy of Finland project 320085. E.L. was supported by Academy of Finland projects 317636 and 320045. Part of the French contribution was supported by the CNRS and the French spatial agency (CNES). Based on observations collected at the Observatorio de Sierra Nevada, owned and operated by the Instituto de Astrofisica de Andalucia (IAA-CSIC). Based on observations collected at the Centro Astronomico Hispano-Aleman (CAHA), proposal 22A-2.2-015, operated jointly by Junta de Andalucia and Consejo Superior de Investigaciones Cientificas (IAA-CSIC).

Extracellular RNA (exRNA) has long been considered as cellular waste that plants can degrade and utilize to recycle nutrients. However, recent findings highlight the need to reconsider the biological significance of RNAs found outside of plant cells. A handful of studies suggest that the exRNA repertoire, which turns out to be an extremely heterogenous group of non-coding RNAs, comprises species as small as a dozen nucleotides to hundreds of nucleotides long. They are found mostly in free form or associated with RNA-binding proteins, while very few are found inside extracellular vesicles (EVs). Despite their low abundance, small RNAs associated with EVs have been a focus of exRNA research due to their putative role in mediating trans-kingdom RNAi. Therefore, non-vesicular exRNAs have remained completely under the radar until very recently. Here we summarize our current knowledge of the RNA species that constitute the extracellular RNAome and discuss mechanisms that could explain the diversity of exRNAs, focusing not only on the potential mechanisms involved in RNA secretion but also on post-release processing of exRNAs. We will also share our thoughts on the putative roles of vesicular and extravesicular exRNAs in plant–pathogen interactions, intercellular communication, and other physiological processes in plants.

Extracellular vesicles (EVs) are nano-sized, lipid compartments that mediate the intercellular transport of lipids, proteins, nucleic acids and metabolites. During infectious diseases, EVs released by host cells promote immune responses, while those released by pathogens attempt to subvert host immunity. There is a growing body of research investigating the role of fungal EVs in plant pathosystems. It is becoming clear that EVs released by fungal phytopathogens play a role during infection through the transport of protein effectors, toxic metabolites and RNA. Here, we discuss recent findings on EVs in fungal phytopathogens, including the methods employed in their isolation, their characterization, contents and functionality, as well as the key questions remaining to be addressed.

Introduction To describe the publication rates and characteristics of PGY2 psychiatric pharmacy residency projects presented as a poster presentation at the annual meetings of the College of Psychiatric and Neurologic Pharmacists (CPNP) from 2002 to 2018. (As of 2022 the organization is under the name, American Association of Psychiatric Pharmacists.) Methods CPNP abstracts from even years were strategically searched in PubMed, Ovid MEDLINE, and Google Scholar. If a publication was identified, additional data were collected for characterization, including study information, journal information, author information, institutional affiliation, publication year, and time to publication. Results A total of 348 abstracts were evaluated. Publication in a journal was achieved for 60 projects (17.2%), with publication rates decreasing from 2012 to 2018. The mean time to publication was 17.3 months after completion of the residency, with most projects published at 8 months. More than half (51.7%) of these projects were published in a psychiatric pharmacy journal affiliated with CPNP. Study designs were predominantly retrospective, observational, cohort studies with a focus on evaluation of a drug therapy outcome. The PGY2 resident was the first author in 90% of the publications. Forty percent included other health care professionals outside of pharmacy as a coauthor. PGY2 residencies affiliated with academic institutions had overall higher publications rates. Discussion Publication rates for PGY2 psychiatric pharmacy residency projects are low and are decreasing over time despite an increasing number of PGY2 psychiatric pharmacy residency programs. This publication rate is lower than that reported in the literature for PGY2 critical care residency programs. The downward trend of publication rates for PGY2 psychiatric pharmacy residency projects is concerning.

Full list of authors: Xie, Fei; Di Marco, Alessandro; La Monaca, Fabio; Liu, Kuan; Muleri, Fabio; Bucciantini, Niccolò; Romani, Roger W.; Costa, Enrico; Rankin, John; Soffitta, Paolo; Bachetti, Matteo; Di Lalla, Niccolò; Fabiani, Sergio; Ferrazzoli, Riccardo; Gunji, Shuichi; Latronico, Luca; Negro, Michela; Omodei, Nicola; Pilia, Maura; Trois, Alessio; Watanabe, Eri; Agudo, Iván; Antonelli, Lucio A.; Baldini, Luca; Baumgartner, Wayne H.; Bellazzini, Ronaldo; Bianchi, Stefano; Bongiorno, Stephen D.; Bonino, Raffaella; Brez, Alessandro; Capitanio, Fiamma; Castellano, Simone; Cavazzuti, Elisabetta; Ciprini, Stefano; De Rosa, Alessandra; Del Monte, Ettore; Di Gesu, Laura; Donnarumma, Immacolata; Doroshenko, Victor; Dovčiak, Michal; Ehlert, Steven R.; Enoto, Teruaki; Evangelista, Yuri; Garcia, Javier A.; Hayashida, Kiyoshi; Heyl, Jeremy; Iwakiri, Wataru; Jorstad, Svetlana G.; Karas, Vladimir; Kitaguchi, Takao; Kolodziejczak, Jeffery J.; Krawczynski, Henric; Liodakis, Ioannis; Maldera, Simone; Manfreda, Alberto; Marin, Frédéric; Marinucci, Andrea; Marscher, Alan P.; Marshall, Herman L.; Massaro, Francesco; Matt, Giorgio; Mitsuishi, Ikuyuki; Mizuno, Tsunefumi; Ng, C. -Y.; O'Dell, Stephen L.; Oppedisano, Chiara; Papitto, Alessandro; Pavlov, George G.; Peirson, Abel L.; Perri, Matteo; Pesce-Rollins, Melissa; Petrucci, Pierre-Olivier; Possenti, Andrea; Poutanen, Juri; Puccetti, Simonetta; Ramsey, Brian D.; Ratheesh, Ajay; Sgró, Carmelo; Slane, Patrick; Spandre, Gloria; Tamagawa, Toru; Tavecchio, Fabrizio; Taverna, Roberto; Tawara, Yuzuru; Tennant, Allyn F.; Thomas, Nicolas E.; Tombesi, Francesco; Tsygankov, Sergey S.; Turolla, Roberto; Vink, Jacco; Weisskopf, Martin C.; Wu, Kinwah; Zane, Silvia., Pulsar wind nebulae are formed when outflows of relativistic electrons and positrons hit the surrounding supernova remnant or interstellar medium at a shock front. The Vela pulsar wind nebula is powered by a young pulsar (B0833-45, aged 11,000 years)1 and located inside an extended structure called Vela X, which is itself inside the supernova remnant2. Previous X-ray observations revealed two prominent arcs that are bisected by a jet and counter jet3,4. Radio maps have shown high linear polarization of 60% in the outer regions of the nebula5. Here we report an X-ray observation of the inner part of the nebula, where polarization can exceed 60% at the leading edge—approaching the theoretical limit of what can be produced by synchrotron emission. We infer that, in contrast with the case of the supernova remnant, the electrons in the pulsar wind nebula are accelerated with little or no turbulence in a highly uniform magnetic field. © 2022, The Author(s), under exclusive licence to Springer Nature Limited., The IXPE is a joint US and Italian mission. The US contribution is supported by the National Aeronautics and Space Administration (NASA) and is led and managed by the Marshall Space Flight Center (MSFC), with industry partner Ball Aerospace (contract NNM15AA18C). The Italian contribution is supported by the Italian Space Agency (Agenzia Spaziale Italiana, ASI) through contract ASI-OHBI-2017-12-I.0, agreements ASI-INAF-2017-12-H0 and ASI-INFN-2017.13-H0, and its Space Science Data Center (SSDC) with agreements ASI-INAF-2022-14-HH.0 and ASI-INFN 2021-43-HH.0, and by the Istituto Nazionale di Astrofisica (INAF) and the Istituto Nazionale di Fisica Nucleare (INFN) in Italy. This research used data products provided by the IXPE Team (MSFC, SSDC, INAF and INFN) that are distributed with additional software tools by the High-Energy Astrophysics Science Archive Research Center (HEASARC), at NASA Goddard Space Flight Center (GSFC). The research at Guangxi University was supported in part by National Natural Science Foundation of China (grant no. 12133003). The research at Boston University was supported in part by National Science Foundation grant AST-2108622. I.A. acknowledges financial support from the Spanish ‘Ministerio de Ciencia e Innovación’ (MCINN) through the ‘Center of Excellence Severo Ochoa’ award for the Instituto de Astrofísica de Andalucía-CSIC (SEV-2017-0709) and through grants AYA2016-80889-P and PID2019-107847RB-C44.

The title homochiral compound, C18H26O6, 1, was examined by single-crystal X-ray crystallography in order to understand its potential as a synthetic building block, particularly in inter- and intramolecular cyclocondensation reactions. It has also proven to be an excellent model for understanding multiple weak donor–acceptor D—H...A interactions involving terminal acetylenes as donors and as acceptors. The asymmetric unit of 1 comprises three almost identical independent molecules, each with the mannitol 2R,3R,4R,5R configuration and different conformations. Like independent molecules align in strands through acetylenic donor C—H...O contacts with equivalent dioxolanyl acceptor groups. Two of the strands are aligned unidirectionally, in parallel, while the third strand aligns perpendicular to the first two, to give interwoven layers in the supramolecular structure. A detailed study of the interdigitation of the second propargyl group from each independent molecule between strands, and of other short interstrand C—H...O contacts, provides new insight into the application of weak hydrogen-bond theory within the context of a conformationally flexible symmetrical molecule. Analyses of the Cambridge Structural Database using Crystal Packing Features and ConQuest search motifs support the importance of the D...A distance parameter, demonstrate the different influences of donor and acceptor types, and reveal the interplay between H...A and D...A contributions in different contact types.

Physicians have a lot of information to cover in a short amount of time. Over time, this creates burnout and compassion fatigue for physicians. However, psychologists are trained and have a unique skillset they can use to help decrease some of these difficulties. This article is an introduction to the general roles of psychologists and some of their unique responsibilities. The other sections in this article will dive into more specific psychological services for medical specialties.

Blood samples were collected from pigs at six abattoirs in the Kampala, Uganda metropolitan area from May 2021 through June 2022, and tested for African swine fever virus. Thirty-one samples with cycle threshold values < 26 from pigs with different geographic origins, clinical and pathologic signs, and Ornithodoros moubata exposure underwent whole genome sequencing. The p72 gene was used to genotype the isolates, and all were found to be genotype IX; whole genome sequences to previous genotype IX isolates confirmed their similarity. Six of the isolates had enough coverage to evaluate single nucleotide polymorphisms (SNPs). Five of the isolates differed from historic regional isolates, but had similar SNPs to one another, and the sixth isolate also differed from historic regional isolates, but also differed from the other five isolates, even though they are all genotype IX. Whole genome sequencing data provide additional detail on viral evolution that can be useful for molecular epidemiology, and understanding the impact of changes in genes to disease phenotypes, and may be needed for vaccine targeting should a commercial vaccine become available. More sequencing of African swine fever virus isolates is needed in Uganda to understand how and when the virus is changing.

X-ray polarization from the Imaging X-ray Polarimetry Explorer (IXPE) provides an important new probe of the geometry of the pulsar emission zone and of particle acceleration in the surrounding pulsar wind nebula (PWN). However, with IXPE's modest ~20-30" spatial resolution, separation of the pulsar signal from the nebula is a challenge. Conventional analysis defines an "off" phase window as pure nebular emission and subtracts its polarization to isolate the phase-varying pulsar ("on-off fitting"). We present a more sensitive scheme that uses external measurements of the nebula structure and pulsar light curve to isolate their contributions to the phase- and spatially-varying polarization via least-squares regression ("simultaneous fitting"). Tests with simulation data show ~30% improvement in pulse phase polarization uncertainties, decreased background systematics, and substantially improved nebular polarization maps. Applying "simultaneous fitting" to early IXPE Crab data extracts additional phase bins with significant polarization. These bins show interesting departures from the well-known optical polarization sweeps, although additional exposure will be needed for precise model confrontation., 7 pages, 6 figures

Full list of authors: Middei, Riccardo; Liodakis, Ioannis; Perri, Matteo; Puccetti, Simonetta; Cavazzuti, Elisabetta; Di Gesu, Laura; Ehlert, Steven R.; Madejski, Grzegorz; Marscher, Alan P.; Marshall, Herman L.; Muleri, Fabio; Negro, Michela; Jorstad, Svetlana G.; Agis-Gonzalez, Beatriz; Agudo, Ivan; Bonnoli, Giacomo; Bernardos, Maria, I; Casanova, Victor; Garcia-Comas, Maya; Husillos, Cesar; Marchini, Alessandro; Sota, Alfredo; Kouch, Pouya M.; Lindfors, Elina; Borman, George A.; Kopatskaya, Evgenia N.; Larionova, Elena G.; Morozova, Daria A.; Savchenko, Sergey S.; Vasilyev, Andrey A.; Zhovtan, Alexey, V; Casadio, Carolina; Escudero, Juan; Myserlis, Ioannis; Hales, Antonio; Kameno, Seiji; Kneissl, Ruediger; Messias, Hugo; Nagai, Hiroshi; Blinov, Dmitry; Bourbah, Ioakeim G.; Kiehlmann, Sebastian; Kontopodis, Evangelos; Mandarakas, Nikos; Romanopoulos, Stylianos; Skalidis, Raphael; Vervelaki, Anna; Masiero, Joseph R.; Mawet, Dimitri; Millar-Blanchaer, Maxwell A.; Panopoulou, Georgia, V; Tinyanont, Samaporn; Berdyugin, Andrei, V; Kagitani, Masato; Kravtsov, Vadim; Sakanoi, Takeshi; Imazawa, Ryo; Sasada, Mahito; Fukazawa, Yasushi; Kawabata, Koji S.; Uemura, Makoto; Mizuno, Tsunefumi; Nakaoka, Tatsuya; Akitaya, Hiroshi; Gurwell, Mark; Rao, Ramprasad; Di Lalla, Niccolo; Cibrario, Nicolo; Donnarumma, Immacolata; Kim, Dawoon E.; Omodei, Nicola; Pacciani, Luigi; Poutanen, Juri; Tavecchio, Fabrizio; Antonelli, Lucio A.; Bachetti, Matteo; Baldini, Luca; Baumgartner, Wayne H.; Bellazzini, Ronaldo; Bianchi, Stefano; Bongiorno, Stephen D.; Bonino, Raffaella; Brez, Alessandro; Bucciantini, Niccolo; Capitanio, Fiamma; Castellano, Simone; Ciprini, Stefano; Costa, Enrico; De Rosa, Alessandra; Del Monte, Ettore; Di Marco, Alessandro; Doroshenko, Victor; Dovciak, Michal; Enoto, Teruaki; Evangelista, Yuri; Fabiani, Sergio; Ferrazzoli, Riccardo; Garcia, Javier A.; Gunji, Shuichi; Hayashida, Kiyoshi; Heyl, Jeremy; Iwakiri, Wataru; Karas, Vladimir; Kitaguchi, Takao; Kolodziejczak, Jeffery J.; Krawczynski, Henric; La Monaca, Fabio; Latronico, Luca; Maldera, Simone; Manfreda, Alberto; Marin, Frederic; Marinucci, Andrea; Massaro, Francesco; Matt, Giorgio; Mitsuishi, Ikuyuki; Ng, C-Y; O'Dell, Stephen L.; Oppedisano, Chiara; Papitto, Alessandro; Pavlov, George G.; Peirson, Abel L.; Pesce-Rollins, Melissa; Petrucci, Pierre-Olivier; Pilia, Maura; Possenti, Andrea; Ramsey, Brian D.; Rankin, John; Ratheesh, Ajay; Romani, Roger W.; Sgro, Carmelo; Slane, Patrick; Soffitta, Paolo; Spandre, Gloria; Tamagawa, Toru; Taverna, Roberto; Tawara, Yuzuru; Tennant, Allyn F.; Thomas, Nicholas E.; Tombesi, Francesco; Trois, Alessio; Tsygankov, Sergey; Turolla, Roberto; Vink, Jacco; Weisskopf, Martin C.; Wu, Kinwah; Xie, Fei; Zane, Silvia.--This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited., Blazars are a class of jet-dominated active galactic nuclei with a typical double-humped spectral energy distribution. It is of common consensus that the synchrotron emission is responsible for the low frequency peak, while the origin of the high frequency hump is still debated. The analysis of X-rays and their polarization can provide a valuable tool to understand the physical mechanisms responsible for the origin of high-energy emission of blazars. We report the first observations of BL Lacertae (BL Lac) performed with the Imaging X-ray Polarimetry Explorer, from which an upper limit to the polarization degree ΠX < 12.6% was found in the 2–8 keV band. We contemporaneously measured the polarization in radio, infrared, and optical wavelengths. Our multiwavelength polarization analysis disfavors a significant contribution of proton-synchrotron radiation to the X-ray emission at these epochs. Instead, it supports a leptonic origin for the X-ray emission in BL Lac. © 2022. The Author(s). Published by the American Astronomical Society., The Imaging X-ray Polarimetry Explorer (IXPE) is a joint US and Italian mission. The US contribution is supported by the National Aeronautics and Space Administration (NASA) and led and managed by its Marshall Space Flight Center (MSFC), with industry partner Ball Aerospace (contract NNM15AA18C). The Italian contribution is supported by the Italian Space Agency (Agenzia Spaziale Italiana, ASI) through contract ASI-OHBI-2017-12-I.0, agreements ASI-INAF-2017-12-H0 and ASI-INFN-2017.13-H0, and its Space Science Data Center (SSDC), and by the Istituto Nazionale di Astrofisica (INAF) and the Istituto Nazionale di Fisica Nucleare (INFN) in Italy. This research used data products provided by the IXPE Team (MSFC, SSDC, INAF, and INFN) and distributed with additional software tools by the High-Energy Astrophysics Science Archive Research Center (HEASARC), at NASA Goddard Space Flight Center (GSFC). We acknowledge financial support from ASI-INAF agreement n. 2022-14-HH.0. The research at Boston University was supported in part by National Science Foundation grant AST-2108622 and NASA Swift Guest Investigator grant 80NSSC22K0537. This research has made use of data from the RoboPol program, a collaboration between Caltech, the University of Crete, IA-FORTH, IUCAA, the MPIfR, and the Nicolaus Copernicus University, which was conducted at Skinakas Observatory in Crete, Greece. The IAA-CSIC coauthors acknowledge financial support from the Spanish "Ministerio de Ciencia e Innovacion" (MCINN) through the "Center of Excellence Severo Ochoa" award for the Instituto de Astrofísica de Andalucía-CSIC (SEV-2017-0709). Acquisition and reduction of the POLAMI, TOP-MAPCAR, and OSN data was supported in part by MICINN through grants AYA2016-80889-P and PID2019-107847RB-C44. The POLAMI observations were carried out at the IRAM 30 m Telescope. IRAM is supported by INSU/CNRS (France), MPG (Germany), and IGN (Spain). This Letter makes use of the following ALMA director's discretionary time data under proposal ESO#2021.A.00016.T. ALMA is a partnership of ESO (representing its member states), NSF (USA), and NINS (Japan), together with NRC (Canada), MOST, and ASIAA (Taiwan), and KASI (Republic of Korea), in cooperation with the Republic of Chile. The Joint ALMA Observatory is operated by ESO, AUI/NRAO, and NAOJ. Some of the data reported here are based on observations obtained at the Hale Telescope, Palomar Observatory as part of a continuing collaboration between the California Institute of Technology, NASA/JPL, Yale University, and the National Astronomical Observatories of China. This research made use of Photutils, an Astropy package for detection and photometry of astronomical sources (Bradley et al. 2019). G.V.P. acknowledges support by NASA through the NASA Hubble Fellowship grant #HST-HF2-51444.001-A awarded by the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS5-26555. The data in this study include observations made with the Nordic Optical Telescope, owned in collaboration by the University of Turku and Aarhus University, and operated jointly by Aarhus University, the University of Turku, and the University of Oslo, representing Denmark, Finland, and Norway, the University of Iceland and Stockholm University at the Observatorio del Roque de los Muchachos, La Palma, Spain, of the Instituto de Astrofisica de Canarias. The data presented here were obtained in part with ALFOSC, which is provided by the Instituto de Astrofísica de Andalucía (IAA) under a joint agreement with the University of Copenhagen and NOT. E.L. was supported by Academy of Finland projects 317636 and 320045. Part of the French contribution is supported by the Scientific Research National Center (CNRS) and the French Spatial Agency (CNES). Some of the data are based on observations collected at the Observatorio de Sierra Nevada, owned and operated by the Instituto de Astrofísica de Andalucía (IAA-CSIC). Further data are based on observations collected at the Centro Astronómico Hispano-Alemán (CAHA), operated jointly by Junta de Andalucía and Consejo Superior de Investigaciones Científicas (IAA-CSIC). D.B., S.K., R.S., and N. M. acknowledge support from the European Research Council (ERC) under the European Unions Horizon 2020 research and innovation program under grant agreement No. 771282. C.C. acknowledges support by the European Research Council (ERC) under the HORIZON ERC Grants 2021 program under grant agreement No. 101040021. The Dipol-2 polarimeter was built in cooperation by the University of Turku, Finland, and the Leibniz Institut für Sonnenphysik, Germany, with support from the Leibniz Association grant SAW-2011-KIS-7. We are grateful to the Institute for Astronomy, University of Hawaii, for the allocated observing time. A.H. acknowledges The National Radio Astronomy Observatory is a facility of the National Science Foundation operated under cooperative agreement by Associated Universities, Inc. This work was supported by JST, the establishment of university fellowships toward the creation of science technology innovation; grant No. JPMJFS2129. This work was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI grant Nos. JP21H01137. This work was also partially supported by Optical and Near-Infrared Astronomy Inter-University Cooperation Program from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan., With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2021-001131-S).

The centre of the Milky Way Galaxy hosts a black hole with a solar mass of about 4 million (Sagittarius A* (Sgr A)) that is very quiescent at present with a luminosity many orders of magnitude below those of active galactic nuclei1. Reflection of X-rays from Sgr A* by dense gas in the Galactic Centre region offers a means to study its past flaring activity on timescales of hundreds and thousands of years2. The shape of the X-ray continuum and the strong fluorescent iron line observed from giant molecular clouds in the vicinity of Sgr A* are consistent with the reflection scenario3,4,5. If this interpretation is correct, the reflected continuum emission should be polarized6. Here we report observations of polarized X-ray emission in the direction of the molecular clouds in the Galactic Centre using the Imaging X-ray Polarimetry Explorer. We measure a polarization degree of 31% ± 11%, and a polarization angle of −48° ± 11°. The polarization angle is consistent with Sgr A* being the primary source of the emission, and the polarization degree implies that some 200 years ago, the X-ray luminosity of Sgr A* was briefly comparable to that of a Seyfert galaxy.

We report the first $> 99\%$ confidence detection of X-ray polarization in BL Lacertae. During a recent X-ray/$\gamma$-ray outburst, a 287 ksec observation (2022 November 27-30) was taken using the Imaging X-ray Polarimetry Explorer ({\it IXPE}), together with contemporaneous multiwavelength observations from the Neil Gehrels {\it Swift} observatory and {\it XMM-Newton} in soft X-rays (0.3--10~keV), {\it NuSTAR} in hard X-rays (3--70~keV), and optical polarization from the Calar Alto, and Perkins Telescope observatories. Our contemporaneous X-ray data suggest that the {\it IXPE} energy band is at the crossover between the low- and high-frequency blazar emission humps. The source displays significant variability during the observation, and we measure polarization in three separate time bins. Contemporaneous X-ray spectra allow us to determine the relative contribution from each emission hump. We find $>99\%$ confidence X-ray polarization $\Pi_{2-4{\rm keV}} = 21.7^{+5.6}_{-7.9}\%$ and electric vector polarization angle $\psi_{2-4{\rm keV}} = -28.7 \pm 8.7^{\circ}$ in the time bin with highest estimated synchrotron flux contribution. We discuss possible implications of our observations, including previous {\it IXPE} BL Lacertae pointings, tentatively concluding that synchrotron self-Compton emission dominates over hadronic emission processes during the observed epochs., Comment: Accepted to ApJL. 16 pages, 8 figures

Refereed/Peer-reviewed Sudden infant death syndrome (SIDS) occurs more often in male than in female infants, suggesting involvement of the X-chromosome. Histopathological studies have suggested that altered expression of the Neurokinin-1 receptor may also play a role in the pathogenesis of SIDS. It was hypothesised that genetic variants in three X-chromosome-encoded microRNA (miRNA/miR), known to down-regulate expression of the Neurokinin-1 receptor, may contribute to SIDS. Aim: To identify sequence variants in the miRNAs within a study cohort (27 cases of SIDS and 28 controls) and determine if there was a difference in the frequencies in male and female SIDS infants. Methods: Genomic DNA prepared from stored blood spots was amplified and sequenced to identify genetic variants in miR500A, miR500B and miR320D2. Results: No novel variants in the miRNAs were identified in our study cohort. We identified one known single-nucleotide polymorphism (SNP) in miR320D2: rs5907732 G/T, in both cases and controls. No significant difference in the SNP frequency was observed between male and female SIDS cases. Conclusion: This pilot study suggests that sequence variants in three miRNAs do not contribute to the reported higher prevalence of SIDS in male infants and do not contribute to the pathogenesis of SIDS in our cohort.

Although vehicles may be used in a wide variety of suicides, this has not been a focus in the forensic literature. Thus, an analysis of coronial autopsy reports at Forensic Science SA, Adelaide, South Australia over a 16-year period from January 2005 to December 2020 was undertaken to provide an overview of cases in which it was considered that a vehicle had been integral to the successful completion of a suicide. De-identified details were collected from all cases in which a vehicle had facilitated or been used as a method of suicide. A number of different types of vehicle-related suicide methods were identified, including cases where vehicles had been used as secure places for suicide or where the vehicle had been used to cause significant blunt force trauma or to enter a lethal environment such as water. Specific examples were taken from the following categories: (1) inhalation of gas, (2) drug toxicity, (3) hanging or ligature strangulation, (4) self-immolation, (5) drowning, (6) vehicle collision, (7) driving off a cliff, (8) jumping or lying in front of a vehicle and (9) the use of more than one method (i.e. complex). This report is not intended to provide an epidemiological analysis of car-related suicides. Instead, the details of selected cases have been used to illustrate the spectrum of methods that may be used in vehicle-assisted suicides.

Forest and freshwater ecosystems are tightly linked and together provide important ecosystem services, but climate change is affecting their species composition, structure, and function. Research at nine US Long Term Ecological Research sites reveals complex interactions and cascading effects of climate change, some of which feed back into the climate system. Air temperature has increased at all sites, and those in the Northeast have become wetter, whereas sites in the Northwest and Alaska have become slightly drier. These changes have altered streamflow and affected ecosystem processes, including primary production, carbon storage, water and nutrient cycling, and community dynamics. At some sites, the direct effects of climate change are the dominant driver altering ecosystems, whereas at other sites indirect effects or disturbances and stressors unrelated to climate change are more important. Long-term studies are critical for understanding the impacts of climate change on forest and freshwater ecosystems.

Many essential cellular processes rely on substrate rotation or translocation by a multi-subunit, ring-type NTPase. A large number of double-stranded DNA viruses, including tailed bacteriophages and herpes viruses, use a homomeric ring ATPase to processively translocate viral genomic DNA into procapsids during assembly. Our current understanding of viral DNA packaging comes from three archetypal bacteriophage systems: cos, pac and phi29. Detailed mechanistic understanding exists for pac and phi29, but not for cos. Here, we reconstituted in vitro a cos packaging system based on bacteriophage HK97 and provided a detailed biochemical and structural description. We used a photobleaching-based, single-molecule assay to determine the stoichiometry of the DNA-translocating ATPase large terminase. Crystal structures of the large terminase and DNA-recruiting small terminase, a first for a biochemically defined cos system, reveal mechanistic similarities between cos and pac systems. At the same time, mutational and biochemical analyses indicate a new regulatory mechanism for ATPase multimerization and coordination in the HK97 system. This work therefore establishes a framework for studying the evolutionary relationships between ATP-dependent DNA translocation machineries in double-stranded DNA viruses.

This study was undertaken to review fatal cases of insulin overdose in South Australia (SA) over a 20-year period to assess rates and characteristics of insulin-related deaths among insulin-dependent diabetics and non-diabetics for all manners of death. Records from the National Coronial Information System (NCIS) and Forensic Science SA (FSSA) were searched for all cases of fatal insulin overdose in South Australia (SA) between 2000 and 2019. Collected variables included age, sex, cause of death, scene findings, manner of death, decedent medical and personal histories, biochemistry, toxicology, histopathology, and autopsy findings. Statistical analyses were performed using R (version 4.1.2). Forty cases of insulin overdose were identified in SA between 2000 and 2019. Twenty-nine cases (72.5%) were suicides, with the remaining cases classified as accidental or undetermined intent. Thirteen of the 22 insulin-dependent diabetics (59%) had a history of depression, 10 of whom had previously demonstrated suicidal ideation. The current study has shown that suicides using insulin among insulin-dependent diabetics are equally as prevalent, if not more so than fatal accidental insulin overdoses. This can largely be attributed to insulin-dependent diabetic access to a potentially lethal substance. Suicide prevention strategies should focus on insulin-dependent diabetics with a history of depression, particularly for those with access to rapid-acting insulin.

OBJECTIVE: To compare the risk of coronavirus disease 2019 (COVID-19) outcomes by antiretroviral therapy (ART) regimens among men with HIV. DESIGN: We included men with HIV on ART in the Veterans Aging Cohort Study who, between February 2020 and October 2021, were 18 years or older and had adequate virological control, CD4 + cell count, and HIV viral load measured in the previous 12 months, and no previous COVID-19 diagnosis or vaccination. METHODS: We compared the adjusted risks of documented severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, COVID-19-related hospitalization, and intensive care unit (ICU) admission by baseline ART regimen: tenofovir alafenamide (TAF)/emtricitabine (FTC), tenofovir disoproxil fumarate (TDF)/FTC, abacavir (ABC)/lamivudine (3TC), and other. We fit pooled logistic regressions to estimate the 18-month risks standardized by demographic and clinical factors. RESULTS: Among 20 494 eligible individuals, the baseline characteristics were similar across regimens, except that TDF/FTC and TAF/FTC had lower prevalences of chronic kidney disease and estimated glomerular filtration rate

The spatial distribution of radiopharmaceuticals within multicellular clusters is known to have a significant effect on their biological response. Most therapeutic radiopharmaceuticals distribute nonuniformly in tissues which makes predicting responses of micrometastases challenging. The work presented here analyzes published temporally dependent nonuniform activity distributions within tumor spheroids treated with actinium-225-DOTA encapsulating liposomes (sup225/supAc-liposomes) and uses these data in MIRDcell V3.11 to calculate absorbed dose distributions and predict biological response. The predicted responses are compared with experimental responses.Four types of liposomes were prepared having membranes with different combinations of release (R) and adhesion (A) properties. The combinations were Rsup-/supAsup-/sup, Rsup-/supAsup+/sup, Rsup+/supAsup-/sup, and Rsup+/supAsup+/sup. These afford different penetrating properties into tissue. The liposomes were loaded with either carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) orsup225/supAc. MDA-MB-231 spheroids were treated with the CFDA-SE-liposomes, harvested at different times, and the time-integrated CFDA-SE concentration at each radial position within the spheroid was determined. This was translated into meansup225/supAc decays/cell versus radial position, uploaded to MIRDcell, and the surviving fraction of cells in spherical multicellular clusters was simulated. The MIRDcell-predicted surviving fractions were compared with experimental fractional-outgrowths of the spheroids following treatment withsup225/supAc-liposomes.The biological responses of the multicellular clusters treated withsup225/supAc-liposomes with physicochemical properties Rsup+/supAsup+/sup, Rsup-/supAsup+/sup, and Rsup-/supAsup-/supwere predicted by MIRDcell with statistically significant accuracy. The prediction for Rsup+/supAsup-/supwas not predicted accurately.In most instances, MIRDcell predicts responses of spheroids treated withsup225/supAc-liposomes that result in different tissue-penetrating profiles of the delivered radionuclides.

Unexpected hepatic failure with liver necrosis is sometimes encountered during a forensic autopsy. Determining the etiology may sometimes be difficult, although increasingly herbal medicines are being implicated. To determine whether such effects might also be caused by foodstuffs, the following in vitro study was undertaken. Four formulations of traditional herbal soup advertised as bak kut teh were prepared and added to cultures of liver carcinoma cells (HepG2). Cell viability was assessed using an MTT colorimetric assay at 48 h demonstrating that all formulations had significant toxicity prior to dilution (p p = 0.023), Formulation #2 30% (p = 0.009), and Formulation #3 41% (p p > 0.05). Formulation 4 showed approximately 83% cell death before dilution (p p = 0.023) and 1:1000 (14% ± 3.8, p = 0.024). This study has shown that herbal foodstuffs such as bak kut teh may be responsible for variable degrees of in vitro hepatotoxicity, thus extending the range of herbal products that may be potentially injurious to the liver. If unexpected liver damage is encountered at autopsy, information on possible recent ingestion of herbal food preparations should be sought, as routine toxicology screening will not identify the active components. Liver damage may therefore be caused not only by herbal medicines but possibly by herbal products contained in food.

The Suwannee Limestone of Florida and Georgia, USA, is a carbonate paleoenvironment with a rich diversity of marine fossils. These record the presence of coral- and seagrass-bearing communities in the western Atlantic from the Eocene/Oligocene boundary to the mid Oligocene (33.9–28.1 Ma). From the Suwannee Limestone, we describe two new species of the cosmopolitan, diverse, and abundant coral genus Acropora (“staghorn corals”), Acropora suwanneensis new species and Acropora upchurchi new species, which predate all other described Acropora species from the Americas and Caribbean by at least 10 million years. Diminutive skeletal structure in both species is indicative of their living in protected calm-water habitats. At their time of occurrence, an Atlantic–Pacific connection was open through Central America. Both species belong to morphological lineages also present in the fossil record of the eastern Atlantic (Europe, or the western Tethys). These lineages no longer occur in the Atlantic Ocean, but they are extant in the Indo-Pacific region, with the closest modern relative of each new species occurring in the Pacific Ocean to central Indo-Pacific. Some species in the two morphological lineages have existed for up to 20+ million years. The new species may have played a part in perpetuating a cosmopolitan Tethyan fauna and expanding Acropora habitats in the western Atlantic, by which they likely also contributed to the renowned species and habitat diversity of Acropora in the modern Indo-Pacific.UUID https://zoobank.org/40118623-8b82-4352-a065-b58da74a5c41

Plant extracellular vesicles (EVs) are membrane bound organelles involved mainly in intercellular communications and defense responses against pathogens. Recent studies have demonstrated the presence of proteins, nucleic acids including small RNAs, and lipids along with other metabolites in plant EVs. In this paper, we described the isolation and characterization of extracellular vesicles from Sorghum bicolor. Nanoparticle tracking analysis, dynamic light scattering, and cryo-electron tomography showed the presence of a heterogeneous population of EVs isolated from the apoplastic wash of sorghum leaves. Cryo-electron microscopy revealed that EVs had a median size of 110 nm and distinct populations of vesicles with single or multiple lipid bilayers and low or high amounts of contents. The heterogeneity was further supported by data showing that only a subset of EVs that were stained with a membrane dye, Potomac Gold, were also stained with the membrane-permeant esterase-dependent dye, Calcein-acetoxymethyl ester. Proteomic analysis identified 437 proteins that were enriched in multiple EV isolations, with the majority of these also being found in the EV proteome of Arabidopsis. These data suggest a partial conservation of EV contents and function between the monocot, sorghum, and a distantly related eudicot, Arabidopsis.

The phase- and energy-resolved polarization measurements of accreting X-ray pulsars (XRPs) allow us to test different theoretical models of their emission, as well as to provide an avenue to determine the emission region geometry. We present the results of the observations of the XRP GX 301-2 performed with the Imaging X-ray Polarimetry Explorer (IXPE). GX 301-2 is a persistent XRP with one of the longest known spin periods of ~680 s. A massive hyper-giant companion star Wray 977 supplies mass to the neutron star via powerful stellar winds. We do not detect significant polarization in the phase-averaged data using spectro-polarimetric analysis, with the upper limit on the polarization degree (PD) of 2.3% (99% confidence level). Using the phase-resolved spectro-polarimetric analysis we get a significant detection of polarization (above 99% c.l.) in two out of nine phase bins and marginal detection in three bins, with a PD ranging between ~3% and ~10%, and a polarization angle varying in a very wide range from ~0 deg to ~160 deg. Using the rotating vector model we obtain constraints on the pulsar geometry using both phase-binned and unbinned analysis getting excellent agreement. Finally, we discuss possible reasons for a low observed polarization in GX 301-2., 10 pages, 10 figures, submitted to A&A

Ned Kelly, an iconic figure in contemporary Australian mythology, was a bushranger (outlaw) who was executed in 1880 for the murder of a serving police officer, Constable Thomas Lonigan. Kelly is often commemorated by tattoos which depict his armour and helmet or his alleged last words of “Such is life”. A study was undertaken from January 1, 2011, to December 31, 2020, at Forensic Science SA, Adelaide, South Australia, of all cases with such tattoos. De-identified case details included the year of death, age, sex and cause and manner of death. There were 38 cases consisting of 10 natural deaths (26.3%) and 28 unnatural (73.7%). The latter included 15 cases of suicide (39.5%), 9 accidents (23.7%) and 4 homicides (10.5%). Of the 19 suicides and homicides, there were 19 males and no females (age range 24–57 years; average 44 years). The number of suicides in the general South Australian forensic autopsy population in 2020 was 216/1492 (14.5%) which was significantly lower than in the study population in which 39.5% of cases were suicides (2.7 times higher; p p

Understanding microhabitat use is needed to make sound conservation decisions for at-risk, patchy-habitat specialists. Rock habitats offer unique microclimatic refugia for wildlife. Eastern Small-footed Bats (Myotis leibii (Audubon and Bachman, 1842)) use rock roosts during the summer, but data on these summer roosts are lacking for this species classified as (critically) imperiled in several US states and Canadian provinces and as globally endangered. Our goal was to characterize the structure and microclimate of M. leibii roosts at the southwestern periphery of their range. We predicted that M. leibii roost temperatures would be warmer and less variable than ambient temperatures and that solitary bats would use horizontal roosts cooler at night, whereas maternity group roosts would be vertical and warmer at night. During summers 2019 and 2020, we recorded physical (e.g., width) and temperature attributes of 58 M. leibii roosts at 16 sites in the Ouachita Mountains. Crevice roosts of M. leibii had narrow dimensions like elsewhere in their range and roost temperatures (measured with iButtons) were warmer and more variable than ambient temperatures. Group roosts were larger and had more stable temperatures than solitary roosts. These findings may be useful for assessing population threats, monitoring roost suitability, identifying roost-rich areas that need protection, and even planning artificial roost structures where natural roosts are limited.

The fear of being buried alive or taphophobia remains a significant concern for a number of individuals. In previous centuries however, reports of live burials were frequently promulgated in the media fostering an industry focused around the manufacturing and selling of security coffins which either facilitated egress or enabled the recently buried to alert those on the surface to their plight. Holding mortuaries with resuscitation facilities were also established mainly in Continental Europe to permit close observation of the recently deceased until definitive signs of putrefaction had developed. Underpinning much of this panic was the inability of medical practitioners to definitely diagnose death. Although still a rare possibility, mainly in situations where qualified medical personnel are not available, the likelihood of alive burial is nowadays fortunately rare.

Following a witnessed lethal lightning strike of an adult male who was standing outside in a storm, numerous Lichtenberg figures were identified upon external examination of the body. Sectioning across multiple areas of linear erythema in the figures showed no subcutaneous hemorrhage. This was later confirmed on histology which showed only subtle dermal capillary dilatation with no interstitial hemorrhage or inflammation in these areas. The only areas of interstitial hemorrhage were present in adjacent scattered punctate burns from arcing. The documented resolution of Lichtenberg figures within hours would be more in keeping with temporary functional capillary dilatation, shown in this case, rather than with tissue alteration by interstitial hemorrhage or inflammation.

A 39-year-old woman who had undergone an uncomplicated elective cesarean section for a low-lying placenta collapsed and died the following day. At autopsy, there was dissection of an aneurysmally-dilated thoracic aorta with 400 mls of fluid and clotted blood in the pericardial sac. There were no features of Marfan syndrome or other connective tissue disorders. Histology revealed thinning of the aortic wall with fragmentation of elastic fibers and no inflammation. Vessels elsewhere were normal. This case demonstrates a rare complication of pregnancy that may not present until after delivery with unexpected collapse and sudden death. Predisposing factors include an increased cardiac output, reduction in systemic vascular resistance, an increase in left ventricular muscle mass, and alterations in serum progesterone and estrogen levels which may cause structural changes to the aortic wall. The possibility of syndromic and familial connective tissue disorders should also be considered.

Radiation-induced bystander effects have been implicated in contributing to the growth delay of disseminated tumor cells (DTC) caused by 223RaCl2, an alpha particle–emitting radiopharmaceutical. To understand how 223RaCl2 affects the growth, we have quantified biological changes caused by direct effects of radiation and bystander effects caused by the emitted radiations on DTC and osteocytes. Characterizing these effects contribute to understanding the efficacy of alpha particle–emitting radiopharmaceuticals and guide expansion of their use clinically. MDA-MB-231 or MCF-7 human breast cancer cells were inoculated intratibially into nude mice that were previously injected intravenously with 50 or 600 kBq/kg 223RaCl2. At 1-day and 3-days postinoculation, tibiae were harvested and examined for DNA damage (γ-H2AX foci) and apoptosis in osteocytes and cancer cells located within and beyond the range (70 μm) of alpha particles emitted from the bone surface. Irradiated and bystander MDA-MB-231 and MCF-7 cells harbored DNA damage. Bystander MDA-MB-231 cells expressed DNA damage at both treatment levels while bystander MCF-7 cells required the higher administered activity. Osteocytes also had DNA damage regardless of inoculated cancer cell line. The extent of DNA damage was quantified by increases in low (1–2 foci), medium (3–5 foci), and high (5+ foci) damage. MDA-MB-231 but not MCF-7 bystander cells showed increases in apoptosis in 223RaCl2-treated animals, as did irradiated osteocytes. In summary, radiation-induced bystander effects contribute to DTC cytotoxicity caused by 223RaCl2.Implications:This observation supports clinical investigation of the efficacy of 223RaCl2 to prevent breast cancer DTC from progressing to oligometastases.

Accreting X-ray pulsars (XRPs) are presumably ideal targets for polarization measurements, as their high magnetic field strength is expected to polarize the emission up to a polarization degree of ~80%. However, such expectations are being challenged by recent observations of XRPs with the Imaging X-ray Polarimeter Explorer (IXPE). Here we report on the results of yet another XRP, EXO 2030+375, observed with IXPE and contemporarily monitored with Insight-HXMT and SRG/ART-XC. In line with recent results obtained with IXPE for similar sources, analysis of the EXO 2030+375 data returns a low polarization degree of 0%-3% in the phase-averaged study and variation in the range 2%-7% in the phase-resolved study. Using the rotating vector model we constrain the geometry of the system and obtain a value for the magnetic obliquity of ~$60^{\circ}$. Considering also the estimated pulsar inclination of ~$130^{\circ}$, this indicates that the magnetic axis swings close to the observer line of sight. Our joint polarimetric, spectral and timing analysis hint to a complex accreting geometry where magnetic multipoles with asymmetric topology and gravitational light bending significantly affect the observed source behavior., A&A accepted. Proofs version

Supplementary Figure S1. Estimating regional tumor volume within transverse sections. Table S1. Tumor dimensions of the human breast cancer cells inoculated into tibiae Supplementary Figure S2. Clonogenic survival of alpha-particle irradiated MDA-MB-231 (solid line) and MCF-7 (dashed line) human breast cancer cells. Table S2. Statistical significance within treatment groups and timepoints forpercentage ofcells withone or more y-H2AX foci Table S3. Statistical significance within treatment groups and timepoints for percentage of bystander cancer cells with 0, 1-2, 3-5 and 5+ y-H2AX foci Table S4. Statistical significance within treatment groups and timepoints for percentage of irradiated cancer cells with 0, 1-2, 3-5 and 5+ y-H2AX foci Table S5. Statistical significance within treatment groups and timepoints for percentage of osteocyte cells with 0, 1-2, 3-5 and 5+ y-H2AX foci Table S6. Statistical significance within treatment groups and timepoints for percentage of cells that are TUNEL+.

The FGFR4/FGF19 signaling axis is overactivated in 20% of liver tumors and currently represents a promising targetable signaling mechanism in this cancer type. However, blocking FGFR4 or FGF19 has proven challenging due to its physiological role in suppressing bile acid synthesis which leads to increased toxic bile acid plasma levels upon FGFR4 inhibition. An FGFR4-targeting antibody, U3-1784, was generated in order to investigate its suitability as a cancer treatment without major side effects.U3-1784 is a high-affinity fully human antibody that was obtained by phage display technology and specifically binds to FGFR4. The antibody inhibits cell signaling by competing with various FGFs for their FGFR4 binding site thereby inhibiting receptor activation and downstream signaling via FRS2 and Erk. The inhibitory effect on tumor growth was investigated in 10 different liver cancer models in vivo. The antibody specifically slowed tumor growth of models overexpressing FGF19 by up to 90% whereas tumor growth of models not expressing FGF19 was unaffected. In cynomolgus monkeys, intravenous injection of U3-1784 caused elevated serum bile acid and liver enzyme levels indicating potential liver damage. These effects could be completely prevented by the concomitant oral treatment with the bile acid sequestrant colestyramine, which binds and eliminates bile acids in the gut. These results offer a new biomarker-driven treatment modality in liver cancer without toxicity and they suggest a general strategy for avoiding adverse events with FGFR4 inhibitors.