14 results on '"Roberta Amoriello"'
Search Results
2. Investigating Serum sHLA-G Cooperation With MRI Activity and Disease-Modifying Treatment Outcome in Relapsing-Remitting Multiple Sclerosis
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Roberta Amoriello, Roberta Rizzo, Alice Mariottini, Daria Bortolotti, Valentina Gentili, Elena Bonechi, Alessandra Aldinucci, Alberto Carnasciali, Benedetta Peruzzi, Anna Maria Repice, Luca Massacesi, Enrico Fainardi, and Clara Ballerini
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multiple sclerosis ,natalizumab ,serum sHLA-G ,cytokines ,disease activity ,Neurology ,Neurology (clinical) - Abstract
Relapsing-remitting multiple sclerosis (RRMS) is a demyelinating disease in which pathogenesis T cells have a major role. Despite the unknown etiology, several risk factors have been described, including a strong association with human leukocyte antigen (HLA) genes. Recent findings showed that HLA class I-G (HLA-G) may be tolerogenic in MS, but further insights are required. To deepen the HLA-G role in MS inflammation, we measured soluble HLA-G (sHLA-G) and cytokines serum level in 27 patients with RRMS at baseline and after 12 and 24 months of natalizumab (NTZ) treatment. Patients were divided into high (sHLA-G>20 ng/ml), medium (sHLA-G between 10 and 20 ng/ml), and low (sHLA-G
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- 2022
3. Adaptation of Commensal Escherichia coli in Tomato Fruits: Motility, Stress, Virulence
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Alberto Vassallo, Roberta Amoriello, Prandvera Guri, Lorenzo Casbarra, Matteo Ramazzotti, Marco Zaccaroni, Clara Ballerini, Duccio Cavalieri, and Massimiliano Marvasi
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General Immunology and Microbiology ,bacterial adaptation ,fruit contamination ,food safety ,Lycopersicum esculentum ,dendritic cells ,General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology - Abstract
Food contamination can be a serious concern for public health because it can be related to the severe spreading of pathogens. This is a main issue, especially in the case of fresh fruits and vegetables; indeed, they have often been associated with gastrointestinal outbreak events, due to contamination with pathogenic bacteria. However, little is known about the physiological adaptation and bacterial response to stresses encountered in the host plant. Thus, this work aimed to investigate the adaptation of a commensal E. coli strain while growing in tomato pericarp. Pre-adapted and non-adapted cells were compared and used to contaminate tomatoes, demonstrating that pre-adaptation boosted cell proliferation. DNA extracted from pre-adapted and non-adapted cells was sequenced, and their methylation profiles were compared. Hence, genes involved in cell adhesion and resistance against toxic compounds were identified as genes involved in adaptation, and their expression was compared in these two experimental conditions. Finally, pre-adapted and non-adapted E. coli were tested for their ability to resist the presence of toxic compounds, demonstrating that adaptation exerted a protective effect. In conclusion, this work provides new information about the physiological adaptation of bacteria colonizing the tomato fruit pericarp.
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- 2023
4. Immunosenescence and Autoimmunity: Exploiting the T-Cell Receptor Repertoire to Investigate the Impact of Aging on Multiple Sclerosis
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Roberta Amoriello, Alice Mariottini, and Clara Ballerini
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Immunosuppression Therapy ,Aging ,Biological Variation, Individual ,Multiple Sclerosis ,disease modifying therapies (DMTs) ,Immunosenescence ,T-Lymphocytes ,Immunology ,Receptors, Antigen, T-Cell ,Autoimmunity ,Review ,RC581-607 ,T cell receptor, senescence, HTS repertoire, multiple sclerosis ,Immunology and Allergy ,Animals ,Humans ,autoimmune diseases ,T cell receptor (TCR) ,Immunologic diseases. Allergy - Abstract
T-cell receptor (TCR) repertoire diversity is a determining factor for the immune system capability in fighting infections and preventing autoimmunity. During life, the TCR repertoire diversity progressively declines as a physiological aging progress. The investigation of TCR repertoire dynamics over life represents a powerful tool unraveling the impact of immunosenescence in health and disease. Multiple Sclerosis (MS) is a demyelinating, inflammatory, T-cell mediated autoimmune disease of the Central Nervous System in which age is crucial: it is the most widespread neurological disease among young adults and, furthermore, patients age may impact on MS progression and treatments outcome. Crossing knowledge on the TCR repertoire dynamics over MS patients’ life is fundamental to investigate disease mechanisms, and the advent of high- throughput sequencing (HTS) has significantly increased our knowledge on the topic. Here we report an overview of current literature about the impact of immunosenescence and age-related TCR dynamics variation in autoimmunity, including MS.
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- 2021
5. Diverse inflammatory threats modulate astrocytes Ca2+ signaling via connexin43 hemichannels in organotypic spinal slices
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Laura Ballerini, Roberta Amoriello, Clara Ballerini, Christian Memo, Agnes Thalhammer, and Giulia Panattoni
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Lipopolysaccharides ,Intravital Microscopy ,Settore BIO/09 - Fisiologia ,Calcium in biology ,Mice ,0302 clinical medicine ,Neuroinflammation ,Anterior Horn Cells ,Immune resident cells ,Gap junctions ,Calcium signaling ,0303 health sciences ,3. Good health ,Cell biology ,Pro-inflammatory cytokines, LPS, Live imaging, Neuroinflammation, Gap junctions, Hemichannels, Spinal neurons, Immune resident cells ,medicine.anatomical_structure ,Spinal Cord ,Cytokines ,medicine.symptom ,Astrocyte ,LPS ,Genetic Vectors ,Central nervous system ,Pro-inflammatory cytokines ,Inflammation ,In Vitro Techniques ,Biology ,Proinflammatory cytokine ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Calcium imaging ,medicine ,Animals ,Calcium Signaling ,RC346-429 ,Molecular Biology ,030304 developmental biology ,Live imaging ,Spinal neurons ,Research ,Hemichannels ,Gap junctions, Hemichannels, Spinal neurons, Immune resident cells ,Mice, Inbred C57BL ,Microscopy, Fluorescence ,Astrocytes ,Connexin 43 ,Neuroinflammatory Diseases ,Neurology. Diseases of the nervous system ,030217 neurology & neurosurgery - Abstract
Neuroinflammation is an escalation factor shared by a vast range of central nervous system (CNS) pathologies, from neurodegenerative diseases to neuropsychiatric disorders. CNS immune status emerges by the integration of the responses of resident and not resident cells, leading to alterations in neural circuits functions. To explore spinal cord astrocyte reactivity to inflammatory threats we focused our study on the effects of local inflammation in a controlled micro-environment, the organotypic spinal slices, developed from the spinal cord of mouse embryos. These organ cultures represent a complex in vitro model where sensory-motor cytoarchitecture, synaptic properties and spinal cord resident cells, are retained in a 3D fashion and we recently exploit these cultures to model two diverse immune conditions in the CNS, involving different inflammatory networks and products. Here, we specifically focus on the tuning of calcium signaling in astrocytes by these diverse types of inflammation and we investigate the mechanisms which modulate intracellular calcium release and its spreading among astrocytes in the inflamed environment. Organotypic spinal cord slices are cultured for two or three weeks in vitro (WIV) and exposed for 6 h to a cocktail of cytokines (CKs), composed by tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1 β) and granulocyte macrophage-colony stimulating factor (GM-CSF), or to lipopolysaccharide (LPS). By live calcium imaging of the ventral horn, we document an increase in active astrocytes and in the occurrence of spontaneous calcium oscillations displayed by these cells when exposed to each inflammatory threat. Through several pharmacological treatments, we demonstrate that intracellular calcium sources and the activation of connexin 43 (Cx43) hemichannels have a pivotal role in increasing calcium intercellular communication in both CKs and LPS conditions, while the Cx43 gap junction communication is apparently reduced by the inflammatory treatments. Supplementary Information The online version contains supplementary material available at 10.1186/s13041-021-00868-6.
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- 2021
6. An innovative and affordable quantitative assessment of human TCR repertoire
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Roberta Amoriello and Clara Ballerini
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lcsh:R5-920 ,T-Lymphocytes ,lcsh:R ,Receptors, Antigen, T-Cell ,lcsh:Medicine ,High-Throughput Nucleotide Sequencing ,General Medicine ,Computational biology ,Biology ,Tcr repertoire ,Gene Rearrangement, T-Lymphocyte ,Real-Time Polymerase Chain Reaction ,General Biochemistry, Genetics and Molecular Biology ,Quantitative assessment ,Commentary ,Humans ,lcsh:Medicine (General) ,TCR repertoire, HTS, new method, quantitative assessment ,Multiplex Polymerase Chain Reaction - Published
- 2020
7. The anti-inflammatory and immune-modulatory effects of OEA limit DSS-induced colitis in mice
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Giuseppina Mattace Raso, Roberta Amoriello, Clara Ballerini, Barbara Rani, Maria Beatrice Passani, Claudio Pirozzi, Adriano Lama, Maria Pina Mollica, Gustavo Provensi, Rosaria Meli, Lama, A., Provensi, G., Amoriello, R., Pirozzi, C., Rani, B., Mollica, M. P., Mattace Raso, G., Ballerini, C., Meli, R., and Passani, M. B.
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0301 basic medicine ,Male ,Anti-Inflammatory Agents ,Oleic Acids ,Pharmacology ,Inflammatory bowel disease ,Mesenteric lymph node ,Inflammasome ,Oleoylethanolamide ,chemistry.chemical_compound ,0302 clinical medicine ,Medicine ,Intestinal barrier ,Dextran Sulfate ,NF-kappa B ,General Medicine ,Colitis ,030220 oncology & carcinogenesis ,Cytokines ,medicine.symptom ,Inflammation Mediators ,PPAR-α ,Signal Transduction ,medicine.drug_class ,Colon ,Inflammation ,RM1-950 ,Anti-inflammatory ,Permeability ,Proinflammatory cytokine ,03 medical and health sciences ,NLR Family, Pyrin Domain-Containing 3 Protein ,Animals ,Immunologic Factors ,PPAR alpha ,Mesenteric lymph nodes ,Palmitoylethanolamide ,business.industry ,medicine.disease ,digestive system diseases ,Mice, Inbred C57BL ,Toll-Like Receptor 4 ,Disease Models, Animal ,030104 developmental biology ,chemistry ,Myeloid Differentiation Factor 88 ,TLR4 ,Therapeutics. Pharmacology ,Lymph Nodes ,business ,Endocannabinoids - Abstract
Fatty acid ethanolamides acting on proliferator-activated receptor (PPAR)-α are among the endogenous lipid molecules that attenuate inflammatory processes and pain sensitivity. Whereas these properties are well-known for palmitoylethanolamide (PEA), the efficacy of oleoylethanolamide (OEA, first described as a satiety hormone synthesized in the jejunum) has been overlooked. In this study, we aimed to evaluate the effect of OEA administration in a mouse model of colitis. C57BL/6J mice were exposed to 2.5% dextran sodium sulphate (DSS) in drinking water for 5 days. Daily i.p. administration of 10 mg/kg OEA started 3 days before DSS and lasted for 12 days. The DSS-untreated control group received only ultrapure water. DSS mice treated with OEA had a significant improvement of disease score. OEA restored mRNA transcription of PPAR-α, of tight junctions and protective factors of colon integrity disrupted by DSS. The improvement correlated with significant decrease of colonic and systemic levels of pro-inflammatory cytokines compared to the DSS group. OEA antiinflammatory effects were mediated by the selective targeting of the TLR4 axis causing a downstream inhibition of nuclear factor kappa B (NF-κB)- MyD88-dependent and NLRP3 inflammation pathways. OEA treatment also inhibited DSS-induced increase of inflammatory cytokines levels in the mesenteric lymph nodes. CONCLUSIONS AND IMPLICATIONS: These results underscore the validity of OEA as a potent protective and anti-inflammatory agent in ulcerative colitis that may be exploited to broaden the pharmacological strategies against inflammatory bowel disease.
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- 2020
8. T-cell delivery of nanoparticles-bound anti-CD20 monoclonal antibody: successful B-cell depletion in the spinal cord during Experimental Autoimmune Encephalomyelitis
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Saer Doumett, Roberta Amoriello, Alessio Mazzoni, Costanza Ravagli, Mario Milco D'Elios, Elena Bonechi, Laura Cappiello, Alberto Carnasciali, Clara Ballerini, and Giovanni Baldi
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0301 basic medicine ,Encephalomyelitis, Autoimmune, Experimental ,nanoparticles loaded T cells, anti CD20, drug delivery, EAE ,medicine.drug_class ,T-Lymphocytes ,T cell ,Immunology ,Central nervous system ,Neuroscience (miscellaneous) ,Monoclonal antibody ,Anti-CD20 ,Antibodies, Monoclonal, Murine-Derived ,Mice ,03 medical and health sciences ,Myelin ,0302 clinical medicine ,medicine ,Animals ,Immunology and Allergy ,Nanoparticles loaded T cells ,Drug delivery ,EAE ,Pharmacology ,Autoimmune disease ,B-Lymphocytes ,biology ,business.industry ,Multiple sclerosis ,Experimental autoimmune encephalomyelitis ,Antigens, CD20 ,medicine.disease ,Peptide Fragments ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,Spinal Cord ,biology.protein ,Female ,Myelin-Oligodendrocyte Glycoprotein ,Antibody ,Nanoparticle Drug Delivery System ,business ,030217 neurology & neurosurgery - Abstract
We developed a nanotechnology based-cell mediated drug delivery system by loading myelin antigen-specific T cells with nanoparticles bound to anti-CD20 monoclonal antibody. Anti-CD20 antibody is a current treatment (ocrelizumab) for multiple sclerosis (MS), a chronic, inflammatory and autoimmune disease of the central nervous system (CNS). CD20-depletion has been associated with efficacy in active relapsing and progressive MS, but may not efficiently target inflammatory cells compartmentalized in the CNS. In our work, the intravenous transfer of T cells containing nanoparticle-anti-CD20 complex in mice causes B cell depletion in the spleen and in the brain, whereas the injection of anti-CD20 alone depletes B cells only in the spleen. Testing this system in Experimental Autoimmune Encephalomyelitis (EAE), animal model of MS, we found that spinal cord B cell depletion ameliorates the disease course and pathology. Graphical Abstract.
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- 2020
9. The TCR Repertoire Reconstitution in Multiple Sclerosis: Comparing One-Shot and Continuous Immunosuppressive Therapies
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Benedetta Mazzanti, Roberta Amoriello, Clara Ballerini, Anna Maria Repice, Elena Bonechi, Riccardo Saccardi, Luca Massacesi, Victor Greiff, Alessandra Aldinucci, Alberto Carnasciali, Benedetta Peruzzi, and Alice Mariottini
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0301 basic medicine ,lcsh:Immunologic diseases. Allergy ,Adult ,CD4-Positive T-Lymphocytes ,Male ,medicine.medical_treatment ,system immunology, T cell subpopulations, T cell repertoire diversity, multiple sclerosis, disease modifying therapies ,Receptors, Antigen, T-Cell, alpha-beta ,Immunology ,Clone (cell biology) ,Hematopoietic stem cell transplantation ,Biology ,CD8-Positive T-Lymphocytes ,multiple sclerosis ,Transplantation, Autologous ,03 medical and health sciences ,0302 clinical medicine ,Natalizumab ,Multiple Sclerosis, Relapsing-Remitting ,medicine ,Immunology and Allergy ,Humans ,Immunologic Factors ,disease-modifying therapies ,Original Research ,Immunosuppression Therapy ,system immunology ,Repertoire ,Multiple sclerosis ,T-cell repertoire diversity ,T-cell receptor ,Hematopoietic Stem Cell Transplantation ,Middle Aged ,medicine.disease ,3. Good health ,030104 developmental biology ,Computational immunology ,Female ,T-cell subpopulations ,lcsh:RC581-607 ,CD8 ,030215 immunology ,medicine.drug - Abstract
Natalizumab (NTZ) and autologous hematopoietic stem cell transplantation (AHSCT) are two successful treatments for relapsing-remitting multiple sclerosis (RRMS), an autoimmune T-cell-driven disorder affecting the central nervous system that is characterized by relapses interspersed with periods of complete or partial recovery. Both RRMS treatments have been documented to impact T-cell subpopulations and the T-cell receptor (TCR) repertoire in terms of clone frequency, but, so far, the link between T-cell naive and memory populations, autoimmunity, and treatment outcome has not yet been established hindering insight into the post-treatment TCR landscape of MS patients. To address this important knowledge gap, we tracked peripheral T-cell subpopulations (naïve and memory CD4+ and CD8+) across 15 RRMS patients before and after two years of continuous treatment (NTZ) and a single treatment course (AHSCT) by high-throughput TCRß sequencing. We found that the two MS treatments left treatment-specific multidimensional traces in patient TCRß repertoire dynamics with respect to clonal expansion, clonal diversity and repertoire architecture. Comparing MS TCR sequences with published datasets suggested that the majority of public TCRs belonged to virus-associated sequences. In summary, applying multi-dimensional computational immunology to a TCRß dataset of treated MS patients, we show that qualitative changes of TCRß repertoires encode treatment-specific information that may be relevant for future clinical trials monitoring and personalized MS follow-up, diagnosis and treatment regimes. Natalizumab (NTZ) and autologous hematopoietic stem cell transplantation (AHSCT) are two successful treatments for relapsing–remitting multiple sclerosis (RRMS), an autoimmune T-cell–driven disorder affecting the central nervous system that is characterized by relapses interspersed with periods of complete or partial recovery. Both RRMS treatments have been documented to impact T-cell subpopulations and the T-cell receptor (TCR) repertoire in terms of clone frequency, but, so far, the link between T-cell naive and memory populations, autoimmunity, and treatment outcome has not yet been established hindering insight into the posttreatment TCR landscape of MS patients. To address this important knowledge gap, we tracked peripheral T-cell subpopulations (naive and memory CD4+ and CD8+) across 15 RRMS patients before and after 2 years of continuous treatment (NTZ) and a single treatment course (AHSCT) by high-throughput TCRβ sequencing. We found that the two MS treatments left treatment-specific multidimensional traces in patient TCRβ repertoire dynamics with respect to clonal expansion, clonal diversity, and repertoire architecture. Comparing MS TCR sequences with published datasets suggested that the majority of public TCRs belonged to virus-associated sequences. In summary, applying multidimensional computational immunology to a TCRβ dataset of treated MS patients, we show that qualitative changes of TCRβ repertoires encode treatment-specific information that may be relevant for future clinical trials monitoring and personalized MS follow-up, diagnosis, and treatment regimens.
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- 2019
10. Study of the anti-inflammatory effects of dihydrotestosterone in human vaginal smooth muscle cells
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Paolo Comeglio, Erica Sarchielli, Mario Maggi, C. Corno, Roberta Amoriello, Annamaria Morelli, Linda Vignozzi, Sandra Filippi, Elisa Maseroli, Clara Ballerini, Giulia Rastrelli, and Ilaria Cellai
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medicine.medical_specialty ,Endocrinology ,Smooth muscle ,business.industry ,medicine.drug_class ,Internal medicine ,Dihydrotestosterone ,Medicine ,business ,Anti-inflammatory ,medicine.drug - Published
- 2019
11. HP-1-1 Anti Inflammatory Effects of Androgenic Signaling in Human Vagina: A Potential Therapeutical Application for the Genitourinary Syndrome of Menopause
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Roberta Amoriello, Erica Sarchielli, Elisa Maseroli, Paolo Comeglio, Giulia Guarnieri, Linda Vignozzi, Ilaria Cellai, Sarah Cipriani, Clara Ballerini, M. Frison, Mario Maggi, Sandra Filippi, and Annamaria Morelli
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business.industry ,Genitourinary system ,medicine.drug_class ,Urology ,Endocrinology, Diabetes and Metabolism ,Physiology ,medicine.disease ,Anti-inflammatory ,Menopause ,Psychiatry and Mental health ,Endocrinology ,medicine.anatomical_structure ,Reproductive Medicine ,Vagina ,Medicine ,business - Published
- 2020
12. Myelin-specific T cells carry and release magnetite PGLA-PEG COOH nanoparticles in the mouse central nervous system
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Mario Milco D'Elios, Roberta Amoriello, D. Saer, Costanza Ravagli, Elena Bonechi, Clara Ballerini, Barbara Valtancoli, Laura Cappiello, Giovanni Baldi, Alessandra Flori, Luca Menichetti, Alessandra Aldinucci, Andrea Bencini, Luca Conti, Pietro Maggi, Marisa Benagiano, and UCL - SSS/IONS/CEMO - Pôle Cellulaire et moléculaire
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medicine.drug_class ,General Chemical Engineering ,Multiple sclerosis ,Central nervous system ,Experimental autoimmune encephalomyelitis ,02 engineering and technology ,General Chemistry ,021001 nanoscience & nanotechnology ,medicine.disease ,Monoclonal antibody ,In vitro ,Cell biology ,03 medical and health sciences ,chemistry.chemical_compound ,Myelin ,0302 clinical medicine ,medicine.anatomical_structure ,chemistry ,Drug delivery ,medicine ,Chemistry (all) ,Chemical Engineering (all) ,0210 nano-technology ,030217 neurology & neurosurgery ,Iron oxide nanoparticles - Abstract
Progress in nanotechnology has determined new strategies concerning drug delivery into the central nervous system for the treatment of degenerative and inflammatory diseases. To date, brain targeting through systemic drug administration, even in a nano-composition, is often unsuccessful. Therefore, we investigated the possibility of loading T lymphocytes with PGLA–PEG COOH magnetite nanoparticles (30 nm), which can be built up to easily bind drugs and monoclonal antibodies, and to exploit the ability of activated T cells to cross the blood–brain barrier and infiltrate the brain parenchyma. Iron oxide nanoparticles have been widely used in biomedical applications due to their theranostic properties and are therefore a well-established nanomaterial. The magnetite core is easily hybridized with polymeric compounds that may enhance the possibility of the nanoparticles entering cells with low phagocytic properties. Taking advantage of these material characteristics, after in vitro assessment of the viability and functionality of nano-loaded MOG35–55 specific T cells, we transferred cells containing the nano-cargo into naïve mice affected by experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis. By means of histological and immunohistological methods, we were able to identify the nano-loaded T cells in the central nervous system. Our data demonstrated that T cells containing nanomaterials hold the possibility of carrying and releasing nanoparticles in the brain.
- Published
- 2017
13. PS-04-006 Immunomodulatory Effects of Dihydrotestosterone (DHT) in Rat Vaginal Smooth Muscle Cells
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Paolo Comeglio, Sandra Filippi, Roberta Amoriello, Martina Maggi, Elisa Maseroli, Linda Vignozzi, Clara Ballerini, Gabriella B. Vannelli, M. Zizza, Annamaria Morelli, and C. Corno
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Psychiatry and Mental health ,medicine.medical_specialty ,Endocrinology ,Reproductive Medicine ,Smooth muscle ,Chemistry ,Urology ,Endocrinology, Diabetes and Metabolism ,Dihydrotestosterone ,Internal medicine ,medicine ,medicine.drug - Published
- 2019
14. PS-04-008 Study of the Anti-inflammatory Effects of Dihydrotestosterone in Human Vaginal Smooth Muscle Cells
- Author
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Sandra Filippi, Paolo Comeglio, Roberta Amoriello, C. Corno, Ilaria Cellai, Linda Vignozzi, Martina Maggi, Annamaria Morelli, Clara Ballerini, Elisa Maseroli, and Erica Sarchielli
- Subjects
medicine.medical_specialty ,medicine.drug_class ,Chemistry ,Urology ,Endocrinology, Diabetes and Metabolism ,Anti-inflammatory ,Psychiatry and Mental health ,Endocrinology ,Reproductive Medicine ,Smooth muscle ,Internal medicine ,Dihydrotestosterone ,medicine ,medicine.drug - Published
- 2019
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