12 results on '"Remo Barnabei"'
Search Results
2. Evaluation of the Analytical Performances of the Biolabo SOLEA 100 Optical Coagulometer and Comparison with the Stago STA-R MAX Analyser in the Determination of PT, APTT, and Fibrinogen
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Pierangelo Bellio, Simonetta De Angelis, Alessandra Piccirilli, Giulio Di Michele, Remo Barnabei, Gianfranco Amicosante, Mariagrazia Perilli, and Giuseppe Celenza
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analytical techniques and equipment ,haematology and coagulation ,mechanical clot detection ,optical clot detection ,validation/evaluation ,Clinical Biochemistry - Abstract
Introduction. The Biolabo Solea 100 is a fully automated coagulation analyser using an optical system to detect coagulation designed to meet the needs of small- and medium-sized laboratories. This study aimed to evaluate the analytical performance in terms of bias, precision, and interference of the Biolabo Solea 100 coagulometer under routine laboratory conditions. In addition, a comparison was made with Stago STA-R MAX. Materials and Methods. Imprecision and bias were evaluated for activated partial thromboplastin time (APTT), fibrinogen (FIB), and prothrombin time (PT) at the medical decision levels. The results of 200, 181, and 206 plasma samples for APTT, FIB, and PT, respectively, were compared with those obtained by Stago STA-R MAX. In addition, the interference level of bilirubin, haemoglobin, triglycerides, and fractionated heparin was evaluated. Results. Repeatability, intermediate imprecision, bias, and total error are overall below the defined limits of acceptability. Of interest is the high degree of agreement between Solea 100 and STA-R MAX with respect to PT (s), which fits perfectly with the theoretical line of identity (y = 0 + 1.00x). No interferences were found within the limits stated by the manufacturer, with some exceptions for APTT with heparin and APTT and PT for higher bilirubin concentrations. Conclusions. In conclusion, the performance of the Solea 100 optical analyser is satisfactory and adequate for the determination of routine coagulation tests. Moreover, they are perfectly comparable to mechanical systems, such as STA-R MAX and other upper-level analysers, even considering the low interference levels under routine conditions.
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- 2022
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3. Transient disappearance of CD19+/CD5+ B-lymphocyte clone in peripheral blood in a patient with CLL during SARS-CoV-2-related mild disease
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Pierangelo Bellio, Mariagrazia Perilli, Remo Barnabei, Giuseppe Celenza, Gianfranco Amicosante, Antonio Cellini, Giulio Di Michele, and Alessandra Piccirilli
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Medicine (General) ,Coronavirus disease 2019 (COVID-19) ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Lymphocyte ,CD5 ,Clone (cell biology) ,Case Report ,Case Reports ,030204 cardiovascular system & hematology ,+ ,CD19 ,SARS‐CoV‐2 ,03 medical and health sciences ,R5-920 ,0302 clinical medicine ,immune system diseases ,hemic and lymphatic diseases ,lymphocytic leukemia ,Medicine ,CD19+/CD5+ clone ,Mild disease ,clone ,biology ,business.industry ,SARS-CoV-2 ,General Medicine ,Peripheral blood ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Immunology ,biology.protein ,business - Abstract
Although lymphopenia is currently considered a good predictor for the prognosis of COVID‐19, it must be critically evaluated in patients with CLL, where other clinical markers should be considered to define the prognosis and treatment., Although lymphopenia is currently considered a good predictor for the prognosis of COVID‐19, it must be critically evaluated in patients with CLL, where other clinical markers should be considered to define the prognosis and treatment.
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- 2021
4. Transient disappearance of CD19+/CD5+ B-Lymphocytes in peripheral blood in a patient with CLL during SARS-CoV-2 related mild disease
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Gianfranco Amicosante, Giulio Di Michele, Mariagrazia Perilli, Pierangelo Bellio, Alessandra Piccirilli, Remo Barnabei, Antonio Cellini, and Giuseppe Celenza
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Spondylodiscitis ,biology ,business.industry ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Antiviral therapy ,medicine.disease ,CD19 ,Peripheral blood ,immune system diseases ,hemic and lymphatic diseases ,Immunology ,biology.protein ,medicine ,CD5 ,business ,Mild disease - Abstract
We report a 64-years old man with B-cell CLL infected by SARS-CoV-2 during his hospitalization for a spondylodiscitis. Because of his health conditions the duration of the antiviral therapy was restricted to one week where we observed a transient disappearance of CD19+/CD5+ B-lymphocytes in peripheral blood.
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- 2020
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5. Cocoa consumption dose-dependently improves flow-mediated dilation and arterial stiffness decreasing blood pressure in healthy individuals
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Davide Grassi, Paolo Di Giosia, Stefano Necozione, Claudio Ferri, Giovambattista Desideri, Herwig Bernaert, Remo Barnabei, and Leen Allegaert
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Adult ,Male ,medicine.medical_specialty ,Endothelium ,Physiology ,arterial function ,blood pressure ,cocoa ,endothelial function ,flavonoids ,Internal Medicine ,Cardiology and Cardiovascular Medicine ,Flow mediated dilation ,Blood Pressure ,Pulse Wave Analysis ,Vascular Stiffness ,Insulin resistance ,Double-Blind Method ,Internal medicine ,medicine ,Humans ,Blood pressure monitoring ,Flavonoids ,Cacao ,Cross-Over Studies ,Endothelin-1 ,business.industry ,food and beverages ,Blood Pressure Monitoring, Ambulatory ,Middle Aged ,Lipid Metabolism ,medicine.disease ,Crossover study ,Healthy Volunteers ,Biotechnology ,Blood pressure ,medicine.anatomical_structure ,Healthy individuals ,Cardiology ,Arterial stiffness ,Endothelium, Vascular ,Plant Preparations ,Insulin Resistance ,business ,Phytotherapy - Abstract
Cocoa flavonoids exert beneficial vascular effects and reduce the risk of cardiovascular morbidity and mortality. Nevertheless, the involved mechanisms have not been clarified and no study has yet focused on the dose-response effects.We aimed to investigate the effects of different doses of cocoa flavonoids on flow-mediated dilation (FMD), endothelin-1 (ET-1), pulse wave velocity (PWV), and SBP and DBP.According to a randomized, double-blind, controlled, cross-over design, 20 healthy volunteers (1.5% improvement in FMD in 20 individuals: 0.99 at alpha = 0.05) were assigned to receive either five treatments with daily intake of 10 g cocoa (0, 80, 200, 500 and 800 mg cocoa flavonoids/day) in five periods lasting 1 week each.Cocoa dose-dependently increased FMD from 6.2% (control) to 7.3, 7.6, 8.1 and 8.2% after the different flavonoid doses, respectively (P 0.0001). Compared with the control, even 80 mg cocoa flavonoids per day increased FMD (P 0.0001). Cocoa dose-dependently decreased PWV (P 0.0001). Cocoa intake decreased office blood pressure (BP) (SBP: -4.8 ± 1.03 mmHg, P 0.0001; DBP: -3.03 ± 1.07 mmHg, P = 0.0011). With respect to control, cocoa ingestion decreased 24-h (P = 0.05) and daytime (P = 0.038) SBP, and 24-h (P = 0.0064), daytime (P = 0.0088) and night-time (P = 0.0352) pulse pressure. Compared with the control, cocoa dose-dependently decreased ET-1 levels [from 17.1 (control) to 15.2, 14.5, 14.2 and 14.1 pg/ml, after the different flavonoid doses, respectively (P for treatment0.05)]. Compared with the control, significant changes were observed for all doses of flavonoids (ET-1; P 0.05).Our study showed for the first time that cocoa dose-dependently improved FMD and decreased PWV and ET-1 also by ameliorating office and monitored BP. Our findings are clinically relevant, suggesting cocoa, with very low calorie intake, might be reasonably incorporated into a dietary approach, representing a consistent tool in cardiovascular prevention.
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- 2015
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6. SAT-211-HEV infection in Italy: Beyond the hepatic disease
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Alessandro Grimaldi, Giovanna Picchi, Lara Marola, Giustino Parruti, Giulio Pisani, Umbertina Villano, Margherita Dalessandro, M. Paoloni, Michele Equestre, N. Iapadre, Cinzia Marcantonio, Antonio Cellini, Lucia Orneto, Paola Chionne, Simonetta Santini, Stefano Necozione, Federica Sozio, A.R. Ciccaglione, Remo Barnabei, Roberto Bruni, P. Cacciatore, A. Ricciardi, Elisabetta Madonna, and Maurizio Mariani
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Hepatology ,business.industry ,Medicine ,Disease ,business ,Virology - Published
- 2019
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7. Development of hepatocellular cancer induced by long term low fat-high carbohydrate diet in a NAFLD/NASH mouse model
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Remo Barnabei, Edoardo Alesse, Alessandra Tessitore, Carlo Capalbo, Germana Cicciarelli, Francesca Zazzeroni, Simona Pompili, Daria Capece, Roberta Sferra, Valentina Mastroiaco, and Antonella Vetuschi
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0301 basic medicine ,medicine.medical_specialty ,Chronic liver disease ,medicine.disease_cause ,Gastroenterology ,03 medical and health sciences ,Liver disease ,0302 clinical medicine ,Internal medicine ,NAFLD ,Nonalcoholic fatty liver disease ,medicine ,HF diet ,business.industry ,hepatic cancer ,NASH ,Cancer ,medicine.disease ,Obesity ,Molecular medicine ,LF-HC diet ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Metabolic syndrome ,Carcinogenesis ,business ,Research Paper - Abstract
// Alessandra Tessitore 1 , Valentina Mastroiaco 1 , Antonella Vetuschi 1 , Roberta Sferra 1 , Simona Pompili 1 , Germana Cicciarelli 1 , Remo Barnabei 2 , Daria Capece 1 , Francesca Zazzeroni 1 , Carlo Capalbo 3 and Edoardo Alesse 1 1 Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100 L’Aquila, Italy 2 S. Salvatore Hospital, Unit of Laboratory Medicine, 67100 L’Aquila, Italy 3 Department of Molecular Medicine, University “La Sapienza”, 00161 Roma, Italy Correspondence to: Alessandra Tessitore, email: alessandra.tessitore@univaq.it Keywords: NAFLD, NASH, hepatic cancer, LF-HC diet, HF diet Received: December 12, 2016 Accepted: May 29, 2017 Published: June 21, 2017 ABSTRACT Nonalcoholic fatty liver disease (NAFLD) is a common chronic liver disease. It can progress to nonalcoholic steatohepatitis (NASH) and, in a percentage of cases, to hepatocarcinogenesis. The strong incidence in western countries of obesity and metabolic syndrome, whose NAFLD is the hepatic expression, is thought to be correlated to consumption of diets characterized by processed food and sweet beverages. Previous studies described high-fat diet-induced liver tumors. Conversely, the involvement of low-fat/high-carbohydrate diet in the progression of liver disease or cancer initiation has not been described yet. Here we show for the first time hepatic cancer formation in low-fat/high-carbohydrate diet fed NAFLD/NASH mouse model. Animals were long term high-fat, low-fat/high-carbohydrate or standard diet fed. We observed progressive liver damage in low-fat/high-carbohydrate and high-fat animals after 12 and, more, 18 months. Tumors were detected in 20% and 50% of high-fat diet fed mice after 12 and 18 months and, interestingly, in 30% of low-fat/high-carbohydrate fed animals after 18 months. No tumors were detected in standard diet fed mice. Global increase of hepatic interleukin-1β, interleukin-6, tumor necrosis factor-α and hepatocyte growth factor was detected in low-fat/high-carbohydrate and high-fat with respect to standard diet fed mice as well as in tumor with respect to non-tumor bearing mice. A panel of 15 microRNAs was analyzed: some of them revealed differential expression in low-fat/high-carbohydrate with respect to high-fat diet fed groups and in tumors. Data here shown provide the first evidence of the involvement of low-fat/high-carbohydrate diet in hepatic damage leading to tumorigenesis.
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- 2016
8. Platelet activation in patients with the Raynaud phenomenon
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Giuliana Properzi, Claudio Ferri, Paolo Giorgini, L. Polidoro, L. Petrazzi, and Remo Barnabei
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medicine.medical_specialty ,business.industry ,Receptor expression ,Asymptomatic ,Platelet Glycoprotein GPIIb-IIIa Complex ,Thromboxane A2 ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Internal medicine ,Internal Medicine ,medicine ,Platelet ,Platelet activation ,medicine.symptom ,business ,Receptor ,PAC-1 - Abstract
Background: The Raynaud phenomenon (RP) is an exaggerated and reversible vasospasm of small arteries triggered by cold or emotional stress. Primary RP (PRP) term is used when the underlying condition is unknown. An altered regulation in vascular tone and/or release of soluble mediators from activated platelets plays a role in PRP through an increased oxidative stress. We assessed platelet activation and oxidative stress in patients with PRP by measuring platelet PAC-1, an index of glycoprotein (Gp) IIb/IIIa receptor activation, thromboxane A2 (TXA2), an index of platelet activation and 8-epi-prostaglandin F2α (8-epi-PGF2α), a marker of endogenous in vivo peroxidation. Methods: Eighteen asymptomatic patients with PRP (age 41.37 ± 16.94 years; 17 women, 1 man) and 18 healthy subjects (age of 35.11 ± 13.16 years; 16 women, 2 men) were studied. PAC-1 was analysed by flow cytometry while circulating TXB2, a stable metabolite of TXA2 and 8-epi-PGF2α levels were assessed by ELISA kit. Results: Our results show a significant platelet activation in PRP patients as indicated by increased PAC-1 expression (65.29 ± 15.24%; P < 0.001), TXB2 (1477.83 ± 454.04 pg/mL; P= 0.003) and 8-epi-PGF2α circulating levels (42.50 ± 14.14 ng/mL; P < 0.001). An inverse correlation between the degree of PAC-1 expression and TXB2 levels (r=−0.527; P= 0.02) was also found in PRP patients, suggesting that downregulation of GpIIb/IIIa receptor expression may occur during thrombocytopoiesis, as a consequence of the chronic exposure to increased TXB2 concentration. Conclusions: Our study for the first time shows a marked activation of GpIIb/IIIa receptor in asymptomatic patients with PRP and supports antiplatelet therapy in PRP patients.
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- 2012
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9. Unusual onset of venous thromboembolism and heparin-induced thrombocytopenia in a patient with essential thrombocythemia
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Alessandro Lucchesi, Guglielmo Mariani, Remo Barnabei, Mario Lapecorella, Gabriella Di Leonardo, Mauro Di Ianni, M. Napolitano, Luigi Dell'Orso, and Gino Coletti
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medicine.medical_specialty ,Deep vein ,Platelet Factor 4 ,Polysaccharides ,hemic and lymphatic diseases ,Heparin-induced thrombocytopenia ,medicine ,Humans ,Aged ,Incidental Findings ,medicine.diagnostic_test ,Heparin ,business.industry ,Essential thrombocythemia ,Pneumonia, Pneumocystis ,Anticoagulants ,Hematology ,General Medicine ,Thrombophlebitis ,medicine.disease ,Thrombocytopenia ,Thrombosis ,Surgery ,Pulmonary embolism ,Bone marrow examination ,Venous thrombosis ,medicine.anatomical_structure ,Fondaparinux ,Female ,Warfarin ,Pulmonary Embolism ,business ,Thrombocythemia, Essential ,medicine.drug - Abstract
Essential thrombocythemia is a hematological disorder characterized by clonal hemopoiesis in the bone marrow and increased number of circulating platelets. It is usually discovered accidentally at the time of routine blood examinations or can become clinically evident with either thrombotic or hemorrhagic complications. In the present article, we describe the case of a 66-year-old woman with pneumonia due to Pneumocystis carinii, who experienced deep vein thrombosis and pulmonary embolism during hospitalization with a subsequent heparin-induced thrombocytopenia. Bone marrow examination performed after clinical improvement revealed the patient to be affected by essential thrombocythemia.
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- 2010
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10. COCOA CONSUMPTION DOSE‐DEPENDENTLY IMPROVES FLOW‐MEDIATED DILATION AND ARTERIAL STIFFNESS IN HEALTHY SUBJECTS
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Remo Barnabei, Paola Cheli, Claudio Ferri, Leen Allegaert, Herwig Bernaert, Giovambattista Desideri, Paolo Di Giosia, Stefano Necozione, and Davide Grassi
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medicine.medical_specialty ,business.industry ,Healthy subjects ,food and beverages ,Flow mediated dilation ,medicine.disease ,Biochemistry ,Internal medicine ,Healthy volunteers ,Genetics ,Cardiology ,medicine ,Arterial stiffness ,business ,Molecular Biology ,Biotechnology - Abstract
To investigate the effects of different doses of cocoa on endothelial function and arterial stiffness. According to a randomized, double-blind, controlled, cross-over design, 20 healthy volunteers ...
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- 2013
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11. Impaired Pulmonary Function in Schoolchildren Exposed to Passive Smoking
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Vincenzo Colorizio, Remo Barnabei, Paolo Pasqualetti, Raffaele Casale, Domenico Colantonio, and Massimo Cialente
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Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Passive smoking ,business.industry ,Urinary system ,Respiratory disease ,Urine ,respiratory system ,medicine.disease_cause ,medicine.disease ,respiratory tract diseases ,Pulmonary function testing ,chemistry.chemical_compound ,FEV1/FVC ratio ,chemistry ,Internal medicine ,medicine ,Physical therapy ,Lung volumes ,Cotinine ,business ,circulatory and respiratory physiology - Abstract
Pulmonary function was evaluated by FEV1, FVC, PEF, MEF50%FVC, MEF25%FVC and MMEF in relation with passive smoking exposure in 143 children aged 6–11 years (mean age =
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- 1991
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12. MicroRNA expression analysis in high fat diet-induced NAFLD-NASH-HCC progression: study on C57BL/6J mice
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Germana Cicciarelli, Daria Capece, Remo Barnabei, Mariafausta Fischietti, Francesca Zazzeroni, Agata Gaggiano, Edoardo Alesse, Filippo Del Vecchio, Roberta Sferra, Valentina Mastroiaco, Alessandra Tessitore, Daniela Verzella, and Antonella Vetuschi
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0301 basic medicine ,DOWN-REGULATION ,Pathology ,Cancer Research ,Cirrhosis ,Liver disorder ,Mice ,Liver disease ,0302 clinical medicine ,Low fat diet ,Non-alcoholic Fatty Liver Disease ,Fibrosis ,HCC ,METABOLIC SYNDROME ,INSULIN-RESISTANCE ,microRNA ,NONALCOHOLIC STEATOHEPATITIS ,High fat diet ,Liver Neoplasms ,Fatty liver ,NASH ,PROSTATE-CANCER ,Gene Expression Regulation, Neoplastic ,Cholesterol ,Oncology ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Disease Progression ,Life Sciences & Biomedicine ,Research Article ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,CELL LUNG-CANCER ,Diet, High-Fat ,03 medical and health sciences ,CIRCULATING MICRORNAS ,LIVER-DISEASE ,NAFLD ,Cell Line, Tumor ,medicine ,Genetics ,BREAST-CANCER ,Animals ,Humans ,Oncology & Carcinogenesis ,Triglycerides ,Science & Technology ,HUMAN HEPATOCELLULAR-CARCINOMA ,medicine.disease ,digestive system diseases ,Disease Models, Animal ,MicroRNAs ,030104 developmental biology ,Steatosis ,Steatohepatitis ,1112 Oncology And Carcinogenesis - Abstract
Background Hepatocellular carcinoma (HCC) is the most common malignant tumor of the liver. Non-alcoholic fatty liver disease (NAFLD) is a frequent chronic liver disorder in developed countries. NAFLD can progress through the more severe non alcoholic steatohepatitis (NASH), cirrhosis and, lastly, HCC. Genetic and epigenetic alterations of coding genes as well as deregulation of microRNAs (miRNAs) activity play a role in HCC development. In this study, the C57BL/6J mouse model was long term high-fat (HF) or low-fat (LF) diet fed, in order to analyze molecular mechanisms responsible for the hepatic damage progression. Methods Mice were HF or LF diet fed for different time points, then plasma and hepatic tissues were collected. Histological and clinical chemistry assays were performed to assess the progression of liver disease. MicroRNAs’ differential expression was evaluated on pooled RNAs from tissues, and some miRNAs showing dysregulation were further analyzed at the individual level. Results Cholesterol, low and high density lipoproteins, triglycerides and alanine aminotransferase increase was detected in HF mice. Gross anatomical examination revealed hepatomegaly in HF livers, and histological analysis highlighted different degrees and levels of steatosis, inflammatory infiltrate and fibrosis in HF and LF animals, demonstrating the progression from NAFLD through NASH. Macroscopic nodules, showing typical neoplastic features, were observed in 20 % of HF diet fed mice. Fifteen miRNAs differentially expressed in HF with respect to LF hepatic tissues during the progression of liver damage, and in tumors with respect to HF non tumor liver specimens were identified. Among them, miR-340-5p, miR-484, miR-574-3p, miR-720, whose expression was never described in NAFLD, NASH and HCC tissues, and miR-125a-5p and miR-182, which showed early and significant dysregulation in the sequential hepatic damage process. Conclusions In this study, fifteen microRNAs which were modulated in hepatic tissues and in tumors during the transition NAFLD-NASH-HCC are reported. Besides some already described, new and early dysregulated miRNAs were identified. Functional analyses are needed to validate the results here obtained, and to better define the role of these molecules in the progression of the hepatic disease. Electronic supplementary material The online version of this article (doi:10.1186/s12885-015-2007-1) contains supplementary material, which is available to authorized users.
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