16 results on '"Rasa Petraityte-Burneikiene"'
Search Results
2. Pet Rats as the Likely Reservoir for Human Seoul Orthohantavirus Infection
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Elisa Heuser, Stephan Drewes, Jakob Trimpert, Dusan Kunec, Calvin Mehl, Marieke de Cock, Ankje de Vries, Christiane Klier, Martin Oskamp, Peter Tenhaken, Fatima Hashemi, Daniela Heinz, Mariana Nascimento, Marc Boelhauve, Rasa Petraityte-Burneikiene, Dina Raafat, Miriam Maas, Detlev Krüger, Andreas Latz, Jörg Hofmann, Gerald Heckel, Johannes Dreesman, and Rainer Ulrich
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600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche ,pet rat ,high-throughput sequencing ,Seoul virus ,Norway rat ,complete coding sequences ,rat surveillance ,zoonoses ,600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten ,Infectious Diseases ,Virology ,570 Life sciences ,biology - Abstract
Seoul orthohantavirus (SEOV) is a rat-associated zoonotic pathogen with an almost worldwide distribution. In 2019, the first autochthonous human case of SEOV-induced hemorrhagic fever with renal syndrome was reported in Germany, and a pet rat was identified as the source of the zoonotic infection. To further investigate the SEOV reservoir, additional rats from the patient and another owner, all of which were purchased from the same vendor, were tested. SEOV RNA and anti-SEOV antibodies were found in both of the patient’s rats and in two of the three rats belonging to the other owner. The complete coding sequences of the small (S), medium (M), and large (L) segments obtained from one rat per owner exhibited a high sequence similarity to SEOV strains of breeder rat or human origin from the Netherlands, France, the USA, and Great Britain. Serological screening of 490 rats from breeding facilities and 563 wild rats from Germany (2007–2020) as well as 594 wild rats from the Netherlands (2013–2021) revealed 1 and 6 seropositive individuals, respectively. However, SEOV RNA was not detected in any of these animals. Increased surveillance of pet, breeder, and wild rats is needed to identify the origin of the SEOV strain in Europe and to develop measures to prevent transmission to the human population.
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- 2023
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3. Highly diversified shrew hepatitis B viruses corroborate ancient origins and divergent infection patterns of mammalian hepadnaviruses
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Breno Frederico de Carvalho Dominguez Souza, Hauke Niekamp, Debby van Riel, Rainer G. Ulrich, Andreas Geipel, Christian Drosten, Nora Goldmann, Kira Alessandra Alicia Theresa Lowjaga, Ayodeji Olayemi, Andrea Rasche, Ramona Kepper, Dieter Glebe, Felix Lehmann, Victor M. Corman, Thijs Kuiken, Andris Kazaks, Yulia A. Vakulenko, Chantal Akoua-Koffi, Vanessa Schulze, Jan Felix Drexler, Alexander König, Elisabeth Fichet-Calvet, Andres Moreira-Soto, Foday Sahr, Anna-Lena Sander, Alexander N. Lukashev, Joachim Geyer, Rasa Petraityte-Burneikiene, Mathias Schlegel, and Virology
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0301 basic medicine ,Hepatitis B virus ,Virulence Factors ,030106 microbiology ,Peptide binding ,Biology ,medicine.disease_cause ,Evolution, Molecular ,03 medical and health sciences ,Viral Envelope Proteins ,SDG 3 - Good Health and Well-being ,Cell Line, Tumor ,biology.animal ,medicine ,Animals ,Humans ,Phylogeny ,Multidisciplinary ,Models, Genetic ,Shrews ,Shrew ,virus diseases ,Biological Sciences ,Hepatitis B ,medicine.disease ,Virology ,Hepatitis D ,digestive system diseases ,030104 developmental biology ,HBeAg ,Viral evolution ,Hepatitis D virus - Abstract
Shrews, insectivorous small mammals, pertain to an ancient mammalian order. We screened 693 European and African shrews for hepatitis B virus (HBV) homologs to elucidate the enigmatic genealogy of HBV. Shrews host HBVs at low prevalence (2.5%) across a broad geographic and host range. The phylogenetically divergent shrew HBVs comprise separate species termed crowned shrew HBV (CSHBV) and musk shrew HBV (MSHBV), each containing distinct genotypes. Recombination events across host orders, evolutionary reconstructions, and antigenic divergence of shrew HBVs corroborated ancient origins of mammalian HBVs dating back about 80 million years. Resurrected CSHBV replicated in human hepatoma cells, but human- and tupaia-derived primary hepatocytes were resistant to hepatitis D viruses pseudotyped with CSHBV surface proteins. Functional characterization of the shrew sodium taurocholate cotransporting polypeptide (Ntcp), CSHBV/MSHBV surface peptide binding patterns, and infection experiments revealed lack of Ntcp-mediated entry of shrew HBV. Contrastingly, HBV entry was enabled by the shrew Ntcp. Shrew HBVs universally showed mutations in their genomic preCore domains impeding hepatitis B e antigen (HBeAg) production and resembling those observed in HBeAg-negative human HBV. Deep sequencing and in situ hybridization suggest that HBeAg-negative shrew HBVs cause intense hepatotropic monoinfections and low within-host genomic heterogeneity. Geographical clustering and low MSHBV/CSHBV-specific seroprevalence suggest focal transmission and high virulence of shrew HBVs. HBeAg negativity is thus an ancient HBV infection pattern, whereas Ntcp usage for entry is not evolutionarily conserved. Shrew infection models relying on CSHBV/MSHBV revertants and human HBV will allow comparative assessments of HBeAg-mediated HBV pathogenesis, entry, and species barriers.
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- 2019
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4. A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen
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Paulius Lukas Tamosiunas, Martin H. Groschup, Kornelija Markuškienė, Marc Hovestädt, Frank Sellrie, Johannes Scholz, Laima Cepulyte, Henry Memczak, Franziska Ramm, Victor M. Corman, Rasa Petraityte-Burneikiene, Jochen Reetz, D. Becher, Jörg A. Schenk, René Ryll, Paul Dremsek, Stefan Kubick, Anika Andersson, Rainer G. Ulrich, Reimar Johne, Hoai Anh Trinh, Barbara Kubickova, and Publica
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Cell-free synthesis ,Cross-reactivity ,HEV-1 ,HEV-2 ,HEV-3 ,HEV-4 ,HEV-7 ,Hepatitis E virus ,Monoclonal antibody ,batHEV ,cvHEV ,ratHEV ,viruses ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Epitope ,Mice ,Cricetinae ,0303 health sciences ,Mice, Inbred BALB C ,biology ,Chemistry ,Antibodies, Monoclonal ,virus diseases ,General Medicine ,Capsid ,Antibody ,600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::615 Pharmakologie, Therapeutik ,Biotechnology ,medicine.drug_class ,CHO Cells ,03 medical and health sciences ,Cricetulus ,Antigen ,medicine ,Escherichia coli ,Animals ,Humans ,030304 developmental biology ,Linear epitope ,030306 microbiology ,Virology ,Biotechnological Products and Process Engineering ,digestive system diseases ,Pepscan ,biology.protein ,Capsid Proteins - Abstract
Abstract To generate a hepatitis E virus (HEV) genotype 3 (HEV-3)–specific monoclonal antibody (mAb), the Escherichia coli–expressed carboxy-terminal part of its capsid protein was used to immunise BALB/c mice. The immunisation resulted in the induction of HEV-specific antibodies of high titre. The mAb G117-AA4 of IgG1 isotype was obtained showing a strong reactivity with the homologous E. coli, but also yeast-expressed capsid protein of HEV-3. The mAb strongly cross-reacted with ratHEV capsid protein derivatives produced in both expression systems and weaker with an E. coli–expressed batHEV capsid protein fragment. In addition, the mAb reacted with capsid protein derivatives of genotypes HEV-2 and HEV-4 and common vole hepatitis E virus (cvHEV), produced by the cell-free synthesis in Chinese hamster ovary (CHO) and Spodoptera frugiperda (Sf21) cell lysates. Western blot and line blot reactivity of the mAb with capsid protein derivatives of HEV-1 to HEV-4, cvHEV, ratHEV and batHEV suggested a linear epitope. Use of truncated derivatives of ratHEV capsid protein in ELISA, Western blot, and a Pepscan analysis allowed to map the epitope within a partially surface-exposed region with the amino acid sequence LYTSV. The mAb was also shown to bind to human patient–derived HEV-3 from infected cell culture and to hare HEV-3 and camel HEV-7 capsid proteins from transfected cells by immunofluorescence assay. The novel mAb may serve as a useful tool for further investigations on the pathogenesis of HEV infections and might be used for diagnostic purposes. Key points • The antibody showed cross-reactivity with capsid proteins of different hepeviruses. • The linear epitope of the antibody was mapped in a partially surface-exposed region. • The antibody detected native HEV-3 antigen in infected mammalian cells.
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- 2021
5. Human parvoviruses affect the development and clinical course of meningitis and meningoencephalitis
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Anda Vilmane, Anna Terentjeva, Paulius Lukas Tamosiunas, Normunds Suna, Inga Suna, Rasa Petraityte-Burneikiene, Modra Murovska, Santa Rasa-Dzelzkaleja, and Zaiga Nora-Krukle
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Background: Meningitis and meningoencephalitis are neurological inflammatory diseases and although routine diagnostics include testing of wide range of pathogens, still in many cases no causative agent is detected. Human parvovirus B19 (B19V), human bocaviruses 1-4 (HBoV1-4) and human parvovirus 4 (hPARV4) are members of Parvoviridae family and are associated with wide range of clinical manifestations including neurological disorders. The main aim of this study was to determine whether human parvoviruses infection markers are present among patients with meningitis/meningoencephalitis in Latvia as well as to clarify the role of these viruses on clinical course of mentioned diseases. Methods: In this study 42 cases of confirmed or unknown aetiology meningitis or meningoencephalitis were evaluated. PCR method was used for detection of B19V, HBoV1-4 and hPARV4 genomic sequences. In order to evaluate if there are some clinical differences between patient groups and in case of active parvovirus infection, nonparametric statistical tests were used. Results: Our study revealed HBoV1-4 and B19V genomic sequences in 52.38% and 16.67% of patients, respectively. Furthermore, symptoms such as the presence of a headache and its severity, fatigue, disorientation and difficulties to concentrate were significantly frequently present in patients with active parvovirus infection in comparison with parvoviruses negative patients. Conclusions: We suggest that HBoV1-4 and B19V infection should be included in the diagnostics to reduce the number of meningitis/meningoencephalitis with unknown/unexplained aetiology.
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- 2020
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6. Human parvoviruses may affect the development and clinical course of meningitis and meningoencephalitis
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Anna Terentjeva, Zaiga Nora-Krukle, Anda Vilmane, Inga Suna, Santa Rasa-Dzelzkaleja, Modra Murovska, Paulius Lukas Tamosiunas, Normunds Suna, and Rasa Petraityte-Burneikiene
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viruses ,human parvovirus 4 ,Article ,lcsh:RC321-571 ,meningitis ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,In patient ,030212 general & internal medicine ,human parvovirus B19 ,human bocaviruses 1–4 ,lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry ,Parvoviridae ,0303 health sciences ,biology ,030306 microbiology ,business.industry ,General Neuroscience ,Human bocavirus ,Parvovirus infection ,Clinical course ,Meningoencephalitis ,virus diseases ,meningoencephalitis ,medicine.disease ,biology.organism_classification ,Immunology ,Etiology ,business ,Meningitis - Abstract
Meningitis and meningoencephalitis are neurological inflammatory diseases, and although routine diagnostics include testing of a wide range of pathogens, still in many cases, no causative agent is detected. Human parvovirus B19 (B19V), human bocaviruses 1&ndash, 4 (HBoV1&ndash, 4), and human parvovirus 4 (hPARV4) are members of the Parvoviridae family and are associated with a wide range of clinical manifestations including neurological disorders. The main aim of this study was to determine whether human parvoviruses infection markers are present among patients with meningitis/meningoencephalitis in Latvia as well as to clarify the role of these viruses on the clinical course of the mentioned diseases. Our study revealed HBoV1&ndash, 4 and B19V genomic sequences in 52.38% and 16.67% of patients, respectively. Furthermore, symptoms such as the presence of a headache and its severity, fatigue, disorientation, and difficulties to concentrate were significantly frequently present in patients with active parvovirus infection in comparison with parvoviruses negative patients, therefore we suggest that HBoV1&ndash, 4 and B19V infection should be included in the diagnostics to reduce the number of meningitis/meningoencephalitis with unknown/unexplained etiology.
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- 2020
7. Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1–4 infections in wild rats from Lithuania
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Arune Verbickaite, Aurelija Zvirbliene, Rasa Petraityte-Burneikiene, Paulius Lukas Tamosiunas, Indre Kucinskaite-Kodze, Martynas Simanavicius, Marius Jasiulionis, Rainer G. Ulrich, and Karolina Juskaite
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0301 basic medicine ,Genotype ,viruses ,030106 microbiology ,Population ,Animals, Wild ,Orthohepevirus ,medicine.disease_cause ,Microbiology ,Serology ,Rodent Diseases ,03 medical and health sciences ,Hepatitis E virus ,medicine ,Animals ,Hepatitis Antibodies ,education ,education.field_of_study ,General Veterinary ,biology ,Phylogenetic tree ,Reverse Transcriptase Polymerase Chain Reaction ,Hepatitis E virus genotype ,virus diseases ,Lithuania ,General Medicine ,biology.organism_classification ,Virology ,digestive system diseases ,Hepatitis E ,Rats ,030104 developmental biology ,Capsid - Abstract
Rat hepatitis E virus (HEV) is an orthohepevirus which is related to other HEV found in humans and other mammals. It was first identified in Norway rats (Rattus norvegicus) from Germany in 2010, and later it has been detected in Black rats (Rattus rattus) and Norway rats from USA, China, Indonesia, Vietnam and many European countries. In this study, we describe molecular and serological investigations of Black and Norway rats trapped in Lithuania, Eastern Europe, for infections with rat HEV and human HEV genotypes 1-4. Rat HEV-specific real-time reverse transcription-PCR (RT-qPCR) analysis of rat liver samples revealed the presence of rat HEV in 9 of 109 (8.3%) samples. In contrast, a RT-qPCR specific for HEV genotypes 1-4 did not reveal any positive samples. A nested broad spectrum RT-PCR was used for a confirmation of rat HEV infection with a subsequent sequencing of the amplified rat HEV genome fragment. Phylogenetic analysis revealed a clustering of all newly identified rat HEV sequences with Norway rat-derived rat HEV sequences from Germany within the species Orthohepevirus C. An indirect ELISA using a yeast-expressed truncated rat HEV capsid protein variant revealed 31.2% seropositive samples indicating a high rate of rat HEV circulation in the rat population examined. In conclusion, the current investigation confirms rat HEV infections in Norway and Black rats in Lithuania, Eastern Europe, and the non-persistent nature of HEV infection.
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- 2018
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8. Validation of the Puumala virus rapid field test for bank voles in Germany
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Elisa Heuser, Stephan Drewes, Stefan Fischer, Jens Jacob, Ulrike Rosenfeld, Daniela Reil, Christian Imholt, Rasa Petraityte-Burneikiene, and Rainer G. Ulrich
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0301 basic medicine ,Veterinary medicine ,Infection risk ,Time Factors ,Epidemiology ,Myodes glareolus ,Puumala virus ,Rodent Diseases ,03 medical and health sciences ,0302 clinical medicine ,Seroepidemiologic Studies ,Germany ,ddc:570 ,Animals ,Seroprevalence ,030212 general & internal medicine ,Institut für Biochemie und Biologie ,Hantavirus ,Immunoassay ,biology ,Arvicolinae ,Diagnostic Tests, Routine ,biology.organism_classification ,Original Papers ,Bank vole ,030104 developmental biology ,Infectious Diseases ,Hemorrhagic Fever with Renal Syndrome ,Antibody detection - Abstract
SUMMARYPuumala virus (PUUV) causes many human infections in large parts of Europe and can lead to mild to moderate disease. The bank vole (Myodes glareolus) is the only reservoir of PUUV in Central Europe. A commercial PUUV rapid field test for rodents was validated for bank-vole blood samples collected in two PUUV-endemic regions in Germany (North Rhine-Westphalia and Baden-Württemberg). A comparison of the results of the rapid field test and standard ELISAs indicated a test efficacy of 93–95%, largely independent of the origin of the antigens used in the ELISA. In ELISAs, reactivity for the German PUUV strain was higher compared to the Swedish strain but not compared to the Finnish strain, which was used for the rapid field test. In conclusion, the use of the rapid field test can facilitate short-term estimation of PUUV seroprevalence in bank-vole populations in Germany and can aid in assessing human PUUV infection risk.
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- 2016
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9. Generation in yeast and antigenic characterization of hepatitis E virus capsid protein virus-like particles
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Martynas Simanavicius, Paulius Lukas Tamosiunas, Rainer G. Ulrich, Indre Kucinskaite-Kodze, Aurelija Zvirbliene, Reimar Johne, and Rasa Petraityte-Burneikiene
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0301 basic medicine ,Viral Hepatitis Vaccines ,Glycosylation ,Genotype ,medicine.drug_class ,viruses ,Blotting, Western ,Enzyme-Linked Immunosorbent Assay ,Saccharomyces cerevisiae ,Biology ,Cross Reactions ,Monoclonal antibody ,medicine.disease_cause ,Antibodies, Viral ,Applied Microbiology and Biotechnology ,Epitope ,Virus ,law.invention ,03 medical and health sciences ,Epitopes ,Mice ,Hepatitis E virus ,law ,medicine ,Animals ,Humans ,Vaccines, Virus-Like Particle ,Antigens, Viral ,Mice, Inbred BALB C ,virus diseases ,Antibodies, Monoclonal ,General Medicine ,Hepatitis E ,medicine.disease ,Virology ,digestive system diseases ,Recombinant Proteins ,Rats ,030104 developmental biology ,Capsid ,Recombinant DNA ,biology.protein ,Capsid Proteins ,Female ,Antibody ,Epitope Mapping ,Biotechnology - Abstract
Hepatitis E is a globally distributed human disease caused by hepatitis E virus (HEV). In Europe, it spreads through undercooked pork meat or other products and with blood components through transfusions. There are no approved or golden standard serologic systems for HEV diagnostics. Commercially available HEV tests often provide inconsistent results which may differ among the assays. In this study, we describe generation in yeast and characterization of HEV genotype 3 (HEV-3) and rat HEV capsid proteins self-assembled into virus-like particles (VLPs) and the development of HEV-specific monoclonal antibodies (MAbs). Full-length HEV-3 and rat HEV capsid proteins and their truncated variants comprising amino acids (aa) 112-608 were produced in yeast S. cerevisiae. The yeast-expressed rat HEV capsid protein was found to be glycosylated. The full-length HEV-3 capsid protein and both full-length and truncated rat HEV capsid proteins were capable to self-assemble into VLPs. All recombinant proteins contained HEV genotype-specific linear epitopes and cross-reactive conformational epitopes recognized by serum antibodies from HEV-infected reservoir animals. Two panels of MAbs against HEV-3 and rat HEV capsid proteins were generated. Their cross-reactivity pattern was investigated by Western blot, ELISA, and immunofluorescence assay on HEV-3-infected cell cultures. The analysis revealed cross-reactive, genotype-specific, and virus-reactive MAbs. MAb epitopes were localized within S, M, and P domains of HEV-3 and rat HEV capsid proteins. Yeast-generated recombinant VLPs of HEV-3 and rat HEV capsid proteins and HEV-specific MAbs might be employed to develop novel HEV detection systems.
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- 2017
10. Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
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Kestutis Sasnauskas, Paula Padula, Rafael A. Medina, Rasa Petraityte-Burneikiene, Alma Gedvilaite, Rainer G. Ulrich, Indre Kucinskaite-Kodze, Marc Mertens, Ausra Razanskiene, Aurelija Zvirbliene, Jonas Schmidt-Chanasit, and Brian Hjelle
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Anticuerpos Monoclonales ,Orthohantavirus ,Sin Nombre virus ,medicine.drug_class ,Hantavirus Infections ,viruses ,Blotting, Western ,Andes virus ,Enzyme-Linked Immunosorbent Assay ,Cross Reactions ,Antibodies, Viral ,Monoclonal antibody ,Puumala virus ,Article ,Virus ,Mice ,Virology ,Chlorocebus aethiops ,medicine ,Animals ,Vero Cells ,Hantavirus ,Roedores ,biology ,Antibodies, Monoclonal ,virus diseases ,General Medicine ,Nucleocapsid Proteins ,biology.organism_classification ,Immunohistochemistry ,Molecular biology ,Humanos ,Bunyaviridae ,Hantavirus Infection ,Inmunohistoquímica - Abstract
Monoclonal antibodies are important tools for various applications in hantavirus diagnostics. Recently, we generated Puumala virus (PUUV)-reactive monoclonal antibodies (mAbs) by immunisation of mice with chimeric polyomavirus-derived virus-like particles (VLPs) harbouring the 120-amino-acid-long amino-terminal region of the PUUV nucleocapsid (N) protein. Here, we describe the generation of two mAbs by co-immunisation of mice with hexahistidine-tagged full-length N proteins of Sin Nombre virus (SNV) and Andes virus (ANDV), their characterization by different immunoassays and comparison with the previously generated mAbs raised against a segment of PUUV N protein inserted into VLPs. All of the mAbs reacted strongly in ELISA and western blot tests with the antigens used for immunization and cross-reacted to varying extents with N proteins of other hantaviruses. All mAbs raised against a segment of the PUUV N protein presented on chimeric VLPs and both mAbs raised against the full-length AND/SNV N protein reacted with Vero cells infected with different hantaviruses. The reactivity of mAbs with native viral nucleocapsids was also confirmed by their reactivity in immunohistochemistry assays with kidney tissue specimens from experimentally SNV-infected rodents and human heart tissue specimens from hantavirus cardiopulmonary syndrome patients. Therefore, the described mAbs represent useful tools for the immunodetection of hantavirus infection. Fil: Kucinskaite-Kodze, Indre. Vilnius University. Institute of Biotechnology; Lituania. Fil: Petraityte-Burneikiene, Rasa. Vilnius University. Institute of Biotechnology; Lituania. Fil: Zvirbliene, Aurelija. Vilnius University. Institute of Biotechnology; Lituania. Fil: Hjelle, Brian. University of New Mexico School of Medicine. Department of Pathology; Estados Unidos. Fil: Medina, Rafael A. University of New Mexico School of Medicine. Department of Pathology; Estados Unidos. Fil: Gedvilaite, Alma. Vilnius University. Institute of Biotechnology; Lituania. Fil: Razanskiene, Ausra. Vilnius University. Institute of Biotechnology; Lituania. Fil: Schmidt-Chanasit, Jonas. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania. Fil: Mertens, Marc. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania. Fil: Padula, Paula. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina. Fil: Sasnauskas, Kestutis. Vilnius University. Institute of Biotechnology; Lituania. Fil: Ulrich, Rainer G. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania.
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- 2010
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11. Saliva as an alternative specimen for detection of Schmallenberg virus-specific antibodies in bovines
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Justas Lazutka, Raimundas Lelešius, Aliona Špakova, Vilimas Sereika, Kestutis Sasnauskas, and Rasa Petraityte-Burneikiene
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Serum ,Immunoglobulin A ,Saliva ,Orthobunyavirus ,Cattle Diseases ,Enzyme-Linked Immunosorbent Assay ,Biology ,Antibodies, Viral ,Bunyaviridae Infections ,Immunoglobulin G ,law.invention ,Indirect ELISA ,fluids and secretions ,Saliva testing ,law ,Animals ,General Veterinary ,Schmallenberg virus ,General Medicine ,biology.organism_classification ,veterinary(all) ,Virology ,Antibody detection ,Milk ,Recombinant DNA ,biology.protein ,Cattle ,Antibody ,Research Article - Abstract
Background Schmallenberg virus (SBV), discovered in continental Europe in late 2011, causes mild clinical signs in adult ruminants, including diarrhoea and reduced milk yield. However, fetal infection can lead to severe malformation in newborn offspring. Enzyme-linked immunosorbent assays (ELISA) are commercially available for detection of SBV-specific antibodies in bovine sera and milk. Here we describe the development and evaluation of an indirect ELISA based on a yeast derived recombinant SBV nucleocapsid protein (N) for the detection of SBV-specific antibodies in bovine saliva. Development of a non-invasive test to detect antibodies in individual bovine saliva samples could potentially provide a test suitable for calves and adult cattle. The aim of this study was to investigate the agreement between the levels of antibodies (IgG) measured in milk and sera, and the level of antibodies (IgG and IgA) in saliva, in comparison with the antibody levels detected in sera and milk with commercially available test. Results Serum, milk and saliva samples from 58 cows were collected from three dairy herds in Lithuania and tested for the presence of SBV-specific antibodies. The presence of IgG antibodies was tested in parallel serum and milk samples, while the presence of IgA and IgG antibodies was tested in saliva samples. The presence of SBV-specific IgG and IgA in saliva was tested using an indirect ELISA based on a yeast-derived recombinant N protein. The presence of SBV-specific IgG in milk and sera was tested in parallel using a commercial recombinant protein based test. The sensitivities of the newly developed tests were as follows: 96 % for the IgG serum assay and 94 % for the IgG milk assay and 85 % and 98 % for IgG and IgA in saliva tests, when compared with data generated by a commercial IgG assay. Conclusions Data from testing the saliva IgG and IgA and also the milk and serum IgG with indirect SBV-specific ELISAs showed close agreement with the commercial serum and milk IgG assay data. The level of IgG in saliva was notably lower in comparison to IgA. The newly developed method exhibits the potential to serve as an easily transferable tool for epidemiological studies. Electronic supplementary material The online version of this article (doi:10.1186/s12917-015-0552-0) contains supplementary material, which is available to authorized users.
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- 2015
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12. Generation in yeast of recombinant virus-like particles of porcine circovirus type 2 capsid protein and their use for a serologic assay and development of monoclonal antibodies
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Vilimas Sereika, Juozas Nainys, Aurelija Zvirbliene, Raimundas Lelešius, Kestutis Sasnauskas, Alma Gedvilaite, Jonas Dabrisius, Rita Lasickiene, and Rasa Petraityte-Burneikiene
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Circovirus ,Swine ,medicine.drug_class ,viruses ,animal diseases ,Enzyme-Linked Immunosorbent Assay ,Saccharomyces cerevisiae ,Antibodies, Viral ,Recombinant virus ,Monoclonal antibody ,Virus ,Serology ,Mice ,medicine ,Animals ,Circoviridae Infections ,Swine Diseases ,Mice, Inbred BALB C ,biology ,Virion ,Antibodies, Monoclonal ,virus diseases ,Virus-like particles ,biology.organism_classification ,Virology ,Porcine circovirus ,Capsid ,biology.protein ,Capsid Proteins ,Monoclonal antibodies ,Porcine circovirus 2 ,Antibody ,Research Article ,Biotechnology - Abstract
Background Porcine circovirus type 2 (PCV2) is considered to be an important emerging pathogen associated with a number of different syndromes and diseases in pigs known as PCV2-associated diseases. It has been responsible for significant mortality among pigs and remains a serious economic problem to the swine industry worldwide leading to significant negative impacts on profitability of pork production. Results In this study we have demonstrated that PCV2 capsid (Cap) protein based virus-like particles (VLPs) were efficiently produced in yeast S. cerevisiae and induced production of monoclonal antibodies (MAbs) reactive with virus-infected cells. Moreover, PCV2 Cap VLPs served as a highly specific recombinant antigen for the development of an indirect IgG PCV2 Cap VLP-based ELISA for the detection of virus-specific IgG antibodies in swine sera. Four hundred-nine serum samples collected from pigs in Lithuania were tested for PCV2-specific IgG to determine the sensitivity and specificity of the newly developed ELISA in parallel using a commercial SERELISA test as a gold standard. From 409 tested serum samples, 297 samples were positive by both assays. Thirty-nine sera from 112 serum samples were determined as negative by SERELISA but were found to be positive both in the newly developed indirect IgG PCV2 Cap VLP-based ELISA and the PCR test. Conclusions We have demonstrated that S. cerevisiae expression system is an alternative to insect/baculovirus expression system for production of homogenous in size and shape PCV2 Cap protein-based VLPs similar to native virions. Yeast expression system tolerated native virus genes encoding PCV2 Cap protein variants as well as the codon-optimized gene. Moreover, yeast-derived PCV2 Cap VLPs were capable to induce the generation of PCV2-specific MAbs that did not show any cross-reactivity with PCV1-infected cells. The high sensitivity and specificity of the indirect IgG PCV2 Cap VLP-based ELISA clearly suggested that this assay is potentially useful diagnostic tool for screening PCV2–suspected samples.
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- 2014
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13. The Use of Chimeric Virus-like Particles Harbouring a Segment of Hantavirus Gc Glycoprotein to Generate a Broadly-Reactive Hantavirus-Specific Monoclonal Antibody
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Alma Gedvilaite, Boris Klempa, Aurelija Zvirbliene, Indre Kucinskaite-Kodze, Rasa Petraityte-Burneikiene, Ausra Razanskiene, and Rainer G. Ulrich
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Virosomes ,medicine.drug_class ,Recombinant Fusion Proteins ,viruses ,Blotting, Western ,lcsh:QR1-502 ,Enzyme-Linked Immunosorbent Assay ,Saccharomyces cerevisiae ,Biology ,Antibodies, Viral ,Monoclonal antibody ,Puumala virus ,lcsh:Microbiology ,Article ,Epitope ,hantavirus ,Hantavirus ,Gc glycoprotein ,Yeast expression system ,Chimeric virus-like particles ,Epitopes ,Mice ,Viral Proteins ,Virology ,medicine ,Animals ,Hamster polyomavirus ,Glycoproteins ,chemistry.chemical_classification ,yeast expression system ,Mice, Inbred BALB C ,chimeric virus-like particles ,Antibodies, Monoclonal ,virus diseases ,biology.organism_classification ,Molecular biology ,Fusion protein ,Infectious Diseases ,Epitope mapping ,chemistry ,monoclonal antibody ,Capsid Proteins ,Polyomavirus ,Glycoprotein ,Epitope Mapping - Abstract
Monoclonal antibodies (MAbs) against viral glycoproteins have important diagnostic and therapeutic applications. In most cases, the MAbs specific to viral glycoproteins are raised against intact virus particles. The biosynthesis of viral glycoproteins in heterologous expression systems such as bacteria, yeast, insect or mammalian cells is often problematic due to their low expression level, improper folding and limited stability. To generate MAbs against hantavirus glycoprotein Gc, we have used initially a recombinant yeast-expressed full-length Puumala virus (PUUV) Gc protein. However, this approach was unsuccessful. As an alternative recombinant antigen, chimeric virus-like particles (VLPs) harboring a segment of PUUV Gc glycoprotein were generated in yeast Saccharomyces cerevisiae. A 99 amino acid (aa)-long segment of Gc protein was inserted into the major capsid protein VP1 of hamster polyomavirus at previously defined positions: either site #1 (aa 80–89) or site #4 (aa 280–289). The chimeric proteins were found to self-assemble to VLPs as evidenced by electron microscopy. Chimeric VLPs induced an efficient insert-specific antibody response in immunized mice. Monoclonal antibody (clone #10B8) of IgG isotype specific to hantavirus Gc glycoprotein was generated. It recognized recombinant full-length PUUV Gc glycoprotein both in ELISA and Western blot assay and reacted specifically with hantavirus-infected cells in immunofluorescence assay. Epitope mapping studies revealed the N-terminally located epitope highly conserved among different hantavirus strains. In conclusion, our approach to use chimeric VLPs was proven useful for the generation of virus-reactive MAb against hantavirus Gc glycoprotein. The generated broadly-reactive MAb #10B8 might be useful for various diagnostic applications.
- Published
- 2014
- Full Text
- View/download PDF
14. Phylogenetic analysis of Puumala virus subtype Bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein
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Marc Mertens, Eveline Kindler, Martin Pfeffer, Martin H. Groschup, Gerhard Dobler, Gerald Heckel, Jutta Esser, Jonas Schmidt-Chanasit, Aurelija Zvirbliene, Christiane Wagner-Wiening, Sandra Essbauer, Roman Wölfel, Rasa Petraityte-Burneikiene, Petra Emmerich, and Rainer G. Ulrich
- Subjects
medicine.drug_class ,Molecular Sequence Data ,Enzyme-Linked Immunosorbent Assay ,Monoclonal antibody ,Antibodies, Viral ,DNA, Mitochondrial ,Puumala virus ,Sensitivity and Specificity ,law.invention ,Antigen ,Phylogenetics ,law ,Virology ,Genetics ,medicine ,Animals ,Humans ,Amino Acid Sequence ,Molecular Biology ,Antigens, Viral ,Phylogeny ,Hantavirus ,biology ,Phylogenetic tree ,Arvicolinae ,Genetic Variation ,Reproducibility of Results ,General Medicine ,Nucleocapsid Proteins ,biology.organism_classification ,Recombinant Proteins ,Phylogeography ,Hemorrhagic Fever with Renal Syndrome ,biology.protein ,Recombinant DNA ,RNA, Viral ,Antibody - Abstract
Puumala virus (PUUV) is the predominant hantavirus species in Germany causing large numbers of mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS). During an outbreak in South-East Germany in 2004 a novel PUUV subtype designated Bavaria was identified as the causative agent of HFRS in humans [1]. Here we present a molecular characterization of this PUUV strain by investigating novel partial and almost entire nucleocapsid (N) protein-encoding small (S-) segment sequences and partial medium (M-) segment sequences from bank voles (Myodes glareolus) trapped in Lower Bavaria during 2004 and 2005. Phylogenetic analyses confirmed their classification as subtype Bavaria, which is further subdivided into four geographical clusters. The entire N protein, harbouring an amino-terminal hexahistidine tag, of the Bavarian strain was produced in yeast Saccharomyces cerevisiae and showed a slightly different reactivity with N-specific monoclonal antibodies, compared to the yeast-expressed N protein of the PUUV strain Vranica/Hallnas. Endpoint titration of human sera from different parts of Germany and from Finland revealed only very slight differences in the diagnostic value of the different recombinant proteins. Based on the novel N antigen indirect and monoclonal antibody capture IgG-ELISAs were established. By using serum panels from Germany and Finland their validation demonstrated a high sensitivity and specificity. In summary, our investigations demonstrated the Bavarian PUUV strain to be genetically divergent from other PUUV strains and the potential of its N protein for diagnostic applications.
- Published
- 2011
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15. Seroprevalence study in forestry workers of a non-endemic region in eastern Germany reveals infections by Tula and Dobrava-Belgrade hantaviruses
- Author
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Kestutis Sasnauskas, Olaf Niederstrasser, Eckhardt Petri, Detlev H. Krüger, Marc Mertens, Rasa Petraityte-Burneikiene, Mario Ziller, Sandra Werdermann, Robert Friedrich, Jörg Hofmann, Rainer G. Ulrich, and Martin H. Groschup
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Microbiology (medical) ,Adult ,Male ,Orthohantavirus ,Hantavirus Infections ,Immunology ,Population ,Immunoblotting ,Enzyme-Linked Immunosorbent Assay ,Antibodies, Viral ,Sensitivity and Specificity ,Young Adult ,Neutralization Tests ,Seroepidemiologic Studies ,Germany ,Immunology and Allergy ,Seroprevalence ,Humans ,Cloning, Molecular ,education ,Antigens, Viral ,Hantavirus ,education.field_of_study ,biology ,Viral Core Proteins ,Outbreak ,Forestry ,General Medicine ,Dobrava-Belgrade virus ,Middle Aged ,biology.organism_classification ,Virology ,Recombinant Proteins ,Occupational Diseases ,Puumala virus ,Capsid Proteins ,Female ,Hantavirus Infection ,Tula virus ,Plasmids - Abstract
Highly endemic and outbreak regions for human hantavirus infections are located in the southern, southeastern, and western parts of Germany. The dominant hantavirus is the bank vole transmitted Puumala virus (PUUV). In the eastern part of Germany, previous investigations revealed Tula virus (TULV) and Dobrava-Belgrade virus (DOBV) infections in the respective rodent reservoirs. Here, we describe a seroprevalence study in forestry workers from Brandenburg, eastern Germany, using IgG ELISA and immunoblot tests based on recombinant TULV, DOBV, and PUUV antigens. Out of the 563 sera tested, 499 from male and 64 from female workers, we found 41 out of the 499 (8.2%) sera from men (mean age 47 years) and 10 out of 64 (15.6%) from the women (mean age 48 years) anti-hantavirus-positive. The majority of the 51 seropositive samples reacted exclusively in the TULV (n=22) and DOBV tests (n=17). Focus reduction neutralization assay investigations on selected sera confirmed the presence of TULV- and DOBV-specific antibodies in the forestry workers. These investigations demonstrated a potential health threat for forestry workers and also the average population in non-endemic geographical regions where TULV and DOBV are circulating in the corresponding reservoir hosts. The infections in this region might be frequently overlooked due to their unspecific and mild symptoms.
- Published
- 2010
16. Generation of recombinant human bocaviruses surface proteins in yeast and their application in serological tests
- Author
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R. Emuzyte, Aiste Bulavaite, Rasa Petraityte-Burneikiene, Paulius Lukas Tamosiunas, Aurelija Zvirbliene, Regina Firantiene, Rita Lasickienė, and Kestutis Sasnauskas
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biology ,law ,Human bocavirus ,Recombinant DNA ,Bioengineering ,General Medicine ,biology.organism_classification ,Molecular Biology ,Virology ,Yeast ,Biotechnology ,law.invention ,Serology - Published
- 2012
- Full Text
- View/download PDF
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