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In this paper we address the potential of a 3 TeV centre-of-mass energy Compact Linear Collider (CLIC) to measure the branching fraction of the Higgs boson decay to two photons, BR($H\rightarrow\gamma\gamma$). Since photons are massless, the Higgs boson coupling to photons is realized through higher order processes involving heavy particles either from the Standard Model or beyond. Any deviation of the measured BR($H\rightarrow\gamma\gamma$), and consequently of the Higgs coupling $g_{H\gamma\gamma}$ from the predictions of the Standard Model, may indicate New Physics. The Higgs decay to two photons is thus an interesting probe of the Higgs sector. This study is performed using simulation of the detector for CLIC and by considering all relevant physics and beam-induced processes in a full reconstruction chain. It is shown that the product of the Higgs production cross-section in $W^+W^-$ fusion and BR($H\rightarrow\gamma\gamma$) can be measured with a relative statistical uncertainty of 5.5\%, assuming the integrated luminosity of 5 ab$^{-1}$ and unpolarized beams.

In this paper we address the potential of a 3 TeV centre-of-mass energy Compact Linear Collider (CLIC) to measure the branching fraction of the Higgs boson decay to two photons, BR($H\rightarrowγγ$). Since photons are massless, the Higgs boson coupling to photons is realized through higher order processes involving heavy particles either from the Standard Model or beyond. Any deviation of the measured BR($H\rightarrowγγ$), and consequently of the Higgs coupling $g_{Hγγ}$ from the predictions of the Standard Model, may indicate New Physics. The Higgs decay to two photons is thus an interesting probe of the Higgs sector. This study is performed using simulation of the detector for CLIC and by considering all relevant physics and beam-induced processes in a full reconstruction chain. It is shown that the product of the Higgs production cross-section in $W^+W^-$ fusion and BR($H\rightarrowγγ$) can be measured with a relative statistical uncertainty of 5.5\%, assuming the integrated luminosity of 5 ab$^{-1}$ and unpolarized beams.

CP violation is one of Sakharov's conditions for the matter-antimatter asymmetry of the Universe. The experimentally observed size of CP violation is insufficient to account for this. Is CP violated in the Higgs sector? Could the SM-like Higgs boson be a mixture of CP even and CP odd states of an extended Higgs sector? With what precision could such effects be measured at future electron-positron colliders? These questions will be discussed in the light of the latest studies at ILC., Comment: Poster presented at the European Physical Society conference on high energy physics (EPS-HEP2021), Online conference, July 26-30, 2021

The Redox Code involves specific, reversible oxidative changes in proteins that modulate protein tertiary structure, interactions, trafficking, and activity, and hence couple the proteome to the metabolic/oxidative state of cells. It is currently a major focus of study in cell biology. Recent studies of dynamic cellular spatial reorganization with MS-based subcellular-spatial-razor proteomics reveal that protein constituents of many subcellular structures, including mitochondria, the endoplasmic reticulum, the plasma membrane, and the extracellular matrix, undergo changes in their subcellular abundance/distribution in response to oxidative stress. These proteins are components of a diverse variety of functional processes spatially distributed across cells. Many of the same proteins are involved in response to suppression of DNA replication indicate that oxidative stress is strongly intertwined with DNA replication/proliferation. Both are replete with networks of moonlighting proteins that show coordinated changes in subcellular location and that include primary protein actuators of the redox code involved in the processing of NAD+ /NADH, NADP+ /NADPH, Cys/CySS, and GSH/GSSG redox couples. Small groups of key proteins such as {KPNA2, KPNB1, PCNA, PTMA, SET} constitute "spatial switches" that modulate many nuclear processes. Much of the functional response involves subcellular protein trafficking, including nuclear import/export processes, vesicle-mediated trafficking, the endoplasmic reticulum/Golgi pathway, chaperone-assisted processes, and other transport systems. This is not visible to measurements of total protein abundance by transcriptomics or proteomics. Comprehensive pictures of cellular function will require collection of data on the subcellular transport and local functions of many moonlighting proteins, especially of those with critical roles in spatial coordination across cells. The proteome-wide analysis of coordinated changes in abundance and trafficking of proteins offered by MS-based proteomics has a unique, crucial role to play in deciphering the complex adaptive systems that underlie cellular function.

Purpose: To assess some terpenes from herbal products for possible inhibitory effects on serum α- amylase in order to ascertain their potential usefulness in the prevention and/or treatment of diabetes Type 2.Methods: Solutions of terpenes (citral, eukalyptol, β-pinene, myrcene, eugenol and terpineol) in deonized water were prepared by ultrasonic and manual mixing in four different concentrations ranging from 0.39 – 5.50 µmol cm-3. Commercial sera (with normal-N and high-H enzyme activity) were used as a source of α-amylase. α-Amylase activity was determined by standard methods using an automated analyzer.Results: All the selected terpenes at their maximal concentrations inhibited α-amylase in N-sera in the range 9.68 – 38.70 and 10.71 - 25.00 % for ultrasonic and manual mixing, respectively, while in H-sera, inhibition was in the range 17.10 - 21.05 and 13.58 – 25.92 % for ultrasonic and manual mixing, respectively. Regardless of the concentration of the inhibitor or the method of mixing, citral was the strongest inhibitor of α-amylase.Conclusion: The selected terpenes, in their appropriate concentrations, influence α-amylase activity to varying degrees. Principal component and agglomerative hierarchical analysis reveal that the most significant factor in α-amylase inhibition is the mode of mixing the samples, rather than their concentrations.Keywords: α-Amylase, β-Pinene, Citral, Diabetes, Essential oil, Eugenol, Eukalyptol, Myrcene, Terpenes, Terpineol

Corticotropin-releasing factor (CRF), urocortin, sauvagine and urotensin I form the CRF family. These peptides bind with different affinities to two subtypes of CRF receptor (CRFR), CRFR1 and CRFR2. The latter exists as two splice variants, the neuronal CRFR2a. and the peripheral CRFR2. CRFR is a G protein-dependent receptor which acts mainly through Gs enhancing cAMP production. However, CRFR1 expressed in neutrophils of the spleen in response to immunologic stimulation and psychological stress does not seem to function through Gs, as indicated by the inability of CRF to stimulate the cAMP production of CRFR1+ neutrophils. Besides the two receptors, a 37 kD CRF binding protein (CRF-BP) binds several CRF peptides with high affinity. CRFR and CRF-BP do not share a common amino acid sequence representing the ligand binding site. In view of the unusually slow offrate of CRF-BP, it is proposed that CRF-BP provides an efficient uptake of free extracellular CRF. Thus, the time of exposure of CRFR to CRF or urocortin can be limited. At this time, the fate of the ligand CRF­ BP complex is unclear. CRFR1 is not only involved in the hypophyseal stimulation of corticotropin release, but hippocampal CRFR1 mediates enhancement of stress-induced learning. CRFR1 may also be involved in basic anxiety. In contrast, at least in the mouse, CRFR2 of the lateral intermediate septum mediates tonic impairment of learning. In response to stressful stimuli or after local injection of high CRF doses, CRFR2 mediates anxiety. Effects requiring CRFR2 can be blocked specifically by the recently developed peptidic antagonist antisauvagine- 30.

The violation of CP symmetry is one of Sakharov's conditions for the matter-antimatter asymmetry of the Universe. Currently known sources of CP violation in the quark and neutrino sectors are insufficient to account for this. Is CP also violated in the Higgs sector? Could the SM-like Higgs boson be a mixture of even and odd CP states of an extended Higgs sector? With what precision could such effects be measured at future electron-positron colliders? These questions will be discussed in the light of the latest and ongoing studies at ILC., Comment: Talk presented at the International Workshop on Future Linear Colliders (LCWS2021), 15-18 March 2021. C21-03-15.1

We have used a proteomics subcellular spatial razor approach to look at changes in total protein abundance and in protein distribution between the nucleus and cytoplasm following exposure of MCF7 breast cancer cells to estradiol. The dominant response of MCF7 cells to estrogen stimulation involves dynamic changes in protein subcellular spatial distribution rather than changes in total protein abundance. Of the 3604 quantitatively monitored proteins, only about 2% show substantial changes in total abundance (>2-fold), whereas about 20% of the proteins show substantial changes in local abundance and/or redistribution of their subcellular location, with up to 16-fold changes in their local concentration in the nucleus or the cytoplasm. We propose that dynamic redistribution of the subcellular location of multiple proteins in response to stimuli is a fundamental characteristic of cells and suggest that perturbation of cellular spatial control may be an important feature of cancer.

Y7, a murine monoclonal IgG1 kappa antibody against a human monoclonal IgM lambda DJ molecule, was affinity purified on an IgM lambda immunoaffinity column. As detected by enzyme-linked immunosorbent assay (ELISA) the isolated Y7 monoclonal antibody was shown to be not cross-reactive with human IgG, human secretory IgA, mu chain, lambda + kappa chains and another human monoclonal IgM lambda BR. Binding to the polyclonal human IgM standard in the same assay was about 30 percent. The epitope specificity of affinity purified and biotinylated Y7 MoAb was localized only in the nonreduced pepsin Fab fragments of IgM lambda DJ immunogen. As the immunogen was determined to be a specific antibody to phosphorylcholine, the specificity of Y7 MoAb was further ascertained in its capacity to induce 95% inhibition of immunogen binding for phosphorylcholine.