44 results on '"Rachel Lefebvre"'
Search Results
2. Neuroma Treatment With the Acellular Nerve Allograft Reconstruction Technique
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Jennifer A Bell, Collean Trotter, Daniel Gittings, Mathew Schur, Kurt M Mohty, Rachel Lefebvre, and Milan Stevanovic
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General Engineering - Published
- 2023
3. Flexor Tenosynovitis Caused by Mycobacterium heraklionense
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Candace Mason, Darren Wong, and Rachel Lefebvre
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Surgery - Published
- 2022
4. Necrotizing Soft Tissue Infection of the Upper Extremity as a Manifestation of Hansen’s Disease
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Sofia Bougioukli, Eva Williams, Ikenna Nwachuku, Kristen Sochol, Milan Stevanovic, and Rachel Lefebvre
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Surgery - Published
- 2022
5. Genome-wide association study for age at puberty and resumption of cyclicity in a crossbred dairy cattle population
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S. Barbey, A.L. Lainé, H. Larroque, Patrice Martin, Rachel Lefebvre, Didier Boichard, Yves Gallard, J.J. Colleau, Génétique Animale et Biologie Intégrative (GABI), Université Paris-Saclay-AgroParisTech-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Domaine expérimental animal du Pin (SEA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
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Periodicity ,Linkage disequilibrium ,Candidate gene ,media_common.quotation_subject ,Population ,Genome-wide association study ,Fertility ,commencement of luteal activity ,Biology ,Quantitative trait locus ,03 medical and health sciences ,Genetic linkage ,Genetics ,Animals ,Sexual Maturation ,education ,Progesterone ,030304 developmental biology ,media_common ,0303 health sciences ,education.field_of_study ,dairy cow ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,Heritability ,040201 dairy & animal science ,[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics ,Cattle ,Female ,Animal Science and Zoology ,age at puberty ,Genome-Wide Association Study ,Food Science - Abstract
International audience; Fertility is of primary economic importance in dairy cattle and the most common reason for involuntary culling. However, standard fertility traits have very low heritability that renders genetic selection slow and difficult. In this study, we explored fertility from an endocrine standpoint. A total of 1,163 crossbred Holstein-Normande females in a 3-generation familial design were studied for progesterone level measured every 10 d to determine age at puberty (PUB) and commencement of postpartum luteal activity (CPLA). Genetic parameters were estimated using REML with WOMBAT software. The heritability estimates were 0.38 ± 0.10 and 0.16 ± 0.07 for PUB and CPLA, respectively. Moreover, the 2 traits were genetically correlated (0.45 ± 0.23), suggesting a partially common determinism. Because of the family structure, a linkage disequilibrium and linkage analysis approach was preferred over standard genome-wide association study to map genomic regions associated with these traits. Ten quantitative trait loci (QTL) were detected for PUB on chromosomes 1, 3, 11, 13, 14, 21, and 29, whereas 3 QTL were associated with CPLA on chromosomes 21 and 26. Only the QTL on chromosome 21 was common to both traits. Four functional candidate genes (NCOA2, GAS2, OVOL1, and FOSL1) were identified in the detected regions. These findings will contribute to a clearer understanding of fertility determinism and enhance the value of introducing endocrinological data in fertility studies.
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- 2021
6. Standardized Whole Blood Assay and Bead-Based Cytokine Profiling Reveal Commonalities and Diversity of the Response to Bacteria and TLR Ligands in Cattle
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Jérémy Lesueur, Sarah Walachowski, Sarah Barbey, Nathan Cebron, Rachel Lefebvre, Frédéric Launay, Didier Boichard, Pierre Germon, Fabien Corbiere, and Gilles Foucras
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Staphylococcus aureus ,Toll-Like Receptors ,Immunology ,Escherichia coli ,Animals ,Cytokines ,Reproducibility of Results ,Streptococcus ,Immunology and Allergy ,Cattle ,Female ,Ligands - Abstract
Recent developments in multiplex technologies enable the determination of a large nu\mber of soluble proteins such as cytokines in various biological samples. More than a one-by-one determination of the concentration of immune mediators, they permit the establishment of secretion profiles for a more accurate description of conditions related to infectious diseases or vaccination. Cytokine profiling has recently been made available for bovine species with the development of a Luminex® technology-based 15-plex assay. Independently from the manufacturer, we evaluated the bovine cytokine/chemokine multiplex assay for limits of detection, recovery rate, and reproducibility. Furthermore, we assessed cytokine secretion in blood samples from 107 cows upon stimulation with heat-killed bacteria and TLR2/4 ligands compared to a null condition. Secretion patterns were analyzed either using the absolute concentration of cytokines or using their relative concentration with respect to the overall secretion level induced by each stimulus. Using Partial Least Square-Discriminant Analysis, we show that the 15-cytokine profile is different under Escherichia coli, Staphylococcus aureus, and Streptococcus uberis conditions, and that IFN-γ, IL-1β, and TNF-α contribute the most to differentiate these conditions. LPS and E. coli induced largely overlapping biological responses, but S. aureus and S. uberis were associated with distinct cytokine profiles than their respective TLR ligands. Finally, results based on adjusted or absolute cytokine levels yielded similar discriminative power, but led to different stimuli-related signatures.
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- 2022
7. Horseshoe abscess of the hand with rice bodies secondary to mycobacterium avium intracellulare infection
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Dakshesh B. Patel, George R. Matcuk, and Rachel Lefebvre
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Wrist Joint ,musculoskeletal diseases ,medicine.medical_specialty ,medicine.medical_treatment ,Mycobacterium avium-intracellulare infection ,Synovectomy ,Wrist ,030218 nuclear medicine & medical imaging ,Arthritis, Rheumatoid ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Abscess ,Mycobacterium avium-intracellulare Infection ,Horseshoe (symbol) ,Aged, 80 and over ,Debridement ,business.industry ,Tenosynovitis ,Little finger ,Hand ,medicine.disease ,Anti-Bacterial Agents ,Surgery ,body regions ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Rheumatoid arthritis ,Female ,business - Abstract
A horseshoe abscess is caused by infection that spreads between the flexor tendon sheath of the thumb or little finger through the radial and ulnar bursae through communication between the two and/or the space of Parona. We present a case of an 80-year-old woman with rheumatoid arthritis who presented with 6 months of right hand and wrist soft tissue swelling, initially treated as a rheumatoid arthritis flare. MRI demonstrates the horseshoe abscess and after surgical irrigation and debridement with synovectomy, cultures demonstrated infection with mycobacterium avium intracellulare (MAI). This case demonstrates the importance of MRI in diagnosing and evaluating the extent of hand infections and for considering mycobacterial organisms for appropriate treatment and antibiotic regimen.
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- 2020
8. Forearm and Arm Tourniquet Tolerance
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Rachel Lefebvre, Landon Cohen, Harrison Ford Kay, Amir Mostofi, Alidad Ghiassi, and Milan Stevanovic
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Upper Extremity ,Forearm ,Arm ,Humans ,Orthopedics and Sports Medicine ,Surgery ,Paresthesia ,Tourniquets - Abstract
In distal upper extremity surgeries, there can be a choice to use an upper arm or forearm tourniquet. This study examines discomfort and tolerance in healthy volunteers to determine whether one is more comfortable.Forty healthy, study participants were randomized to an upper extremity laterality and site. Tourniquets were inflated to 100 mm Hg over systolic blood pressure. Participants experienced an upper arm and a forearm tourniquet sequentially. Visual analog scores (VAS) were recorded at 2-minute intervals. Time until request and VAS at tourniquet deflation were recorded. Time until the complete resolution of paresthesias was also recorded. Participants subjectively stated which tourniquet felt more comfortable.Tourniquets were inflated longer on the forearm than the upper arm (mean 16.1 minutes versus 12.2 minutes; P0.0001). VAS at tourniquet removal was not different between the sites (means 7.3 and 7.3) (P = 0.839). Time until paresthesia resolution after the tourniquet was deflated was not different (means 8.1 and 7.7 minutes) (P = 0.675). Time until paresthesia resolution was proportional to tourniquet inflation time for both sites (regression coefficient 0.41; P0.00001). Participants found the forearm more comfortable (95% confidence interval, 0.63 to 0.92).Forearm placement allows the tourniquet to be inflated for an average of 4 minutes longer. Forearm tourniquet is subjectively more comfortable.
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- 2021
9. Differentiating Upper Extremity Necrotizing Soft Tissue Infection From Serious Cellulitis and Abscess
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Alidad Ghiassi, Landon E Cohen, Milan Stevanovic, Rachel Lefebvre, Samuel A. Cohen, Kristen M Sochol, and Hyunwoo P Kang
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medicine.medical_specialty ,Multivariate analysis ,business.industry ,necrotizing ,General Engineering ,Vital signs ,abscess ,Retrospective cohort study ,medicine.disease ,Logistic regression ,infection ,symbols.namesake ,Orthopedics ,Internal medicine ,Cellulitis ,medicine ,symbols ,cellulitis ,Abscess ,business ,Fasciitis ,soft tissue ,Fisher's exact test - Abstract
Introduction Necrotizing soft tissue infection (NSTI) of the upper extremity (UE) is a rapidly progressing infection that requires early diagnosis and emergent treatment to decrease risks of loss of limb or life. Clinical presentation, particularly of early NSTI, can appear similar to serious cellulitis or abscess. The purpose of this study was to identify factors that are associated with NSTI rather than serious cellulitis and abscess to differentiate patients with similar clinical presentations. Methods This study uses a retrospective cohort design that compares patients ultimately diagnosed with UE NSTI versus those diagnosed with UE serious cellulitis or abscess. Cohorts were matched using the Laboratory Risk Indicators for Necrotizing Fasciitis (LRINEC) score in the setting of UE soft tissue infection. Laboratory values, vital signs, subjective symptoms, and social factors including substance abuse and domiciled status were recorded. Continuous variables were compared using the Mann-Whitney U test, whereas categorical variables were compared using the chi-squared test or the Fisher exact test (for expected values less than 5). A binary logistic regression for continuous and categorical variables was also performed. Significance was set at p
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- 2021
10. Risk Factors Associated With Progression to Surgical Release After Injection of Trigger Digits
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H. Paco Kang, Kurt Mohty, Ali Azad, Venus Vakhshori, and Rachel Lefebvre
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Male ,medicine.medical_specialty ,medicine.drug_class ,Alcohol abuse ,Injections ,Text mining ,Adrenal Cortex Hormones ,Risk Factors ,medicine ,Humans ,Orthopedics and Sports Medicine ,Prospective Studies ,Prospective cohort study ,Depression (differential diagnoses) ,business.industry ,medicine.disease ,Numerical digit ,Surgery ,Trigger Finger Disorder ,Current Procedural Terminology ,Corticosteroid ,Female ,Trigger finger ,business ,Research Article - Abstract
Introduction The mainstay of trigger finger treatment is a corticosteroid injection of the affected digits and is associated with a very high success rate. However, some patients do not respond to nonsurgical management and undergo subsequent surgical release. The purpose of this study is to investigate the comorbidities that predispose patients to progressing from injection to surgical release. Methods Patient data were obtained from a national insurance database. All patients aged 20 years or older who underwent trigger digit injection were included. Any injection that did not specify the digit was excluded. Subsequent procedures, including repeat injection and surgical release, were identified using relevant Current Procedural Terminology codes. A multivariate model was constructed to evaluate potential risk factors for requiring release after prior injection of the same digit. Stepwise backward selection was used to retain significant variables. Results A total of 42,537 trigger digits were identified in 31,830 patients, most of whom were female. The right hand was affected more commonly than the left. The middle and ring fingers were the most commonly affected digits. Over 80% of all trigger digits underwent only a single injection, and approximately 90% of injected digits did not require subsequent release. In the multivariate model, factors associated with higher risk of release were male sex, involvement of additional digits, multiple injections of the same digit, chronic pulmonary disease, HIV/AIDS, obesity, alcohol abuse, and depression. The model also found small fingers to be less likely to progress to release. Discussion Patients with the risk factors identified in this study are more likely to progress to surgical release after trigger finger injection. Although prospective studies are required, the information may be beneficial in counseling patients and their treatment options.
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- 2021
11. Google trends as a tool for evaluating public interest in total knee arthroplasty and total hip arthroplasty
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Samuel A, Cohen, Landon E, Cohen, Jonathan D, Tijerina, Gabriel, Bouz, Rachel, Lefebvre, Milan, Stevanovic, and Nathanael D, Heckmann
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musculoskeletal diseases ,surgical procedures, operative ,Google trends ,Original Article ,knee arthroplasty ,musculoskeletal system ,hip arthroplasty ,Google ,public interest - Abstract
Background and Aims: There are approximately 1 million total knee arthroplasty (TKA) and total hip arthroplasty (THA) procedures performed annually in the United States. With this number projected to increase, it is vital for orthopedic surgeons and health-care systems to properly anticipate healthcare utilization related to TKA and THA. Google Trends (GT) is a free, open source tool that provides customizable analysis of search terms entered into the Google search engine. We aim to explore the relationship between public interest in TKA and THA as determined by GT data and volume of TKA and THA procedures. Methods: GT data were compiled for ten search terms related to TKA and ten search terms related to THA from January 2009 to December 2017. Annual case volumes for TKA/THA procedures were obtained from the Healthcare Cost and Utilization Project National Inpatient Sample from 2009 to 2017. Trend analysis was performed using univariate linear regression of GT data and TKA/THA case volumes. Results: There was a statistically significant positive correlation between GT data and procedure volume for 14 of the 20 search terms studied. Seven TKA-related search terms with a positive correlation to procedure volumes include “total knee replacement,” “knee replacement,” “knee osteoarthritis,” “knee ache,” “knee swelling,” “knee stiffness,” and “chronic knee pain.” Seven THA-related search terms with a positive correlation to procedure volumes include “hip arthroplasty,” “total hip replacement,” “hip replacement,” “hip osteoarthritis,” “hip ache,” “hip swelling,” and “chronic hip pain.” Conclusion: GT may provide a high utility as a convenient and informative data set for orthopedic surgeons to analyze public interest in TKA and THA procedures. The data provided by GT have the potential to provide real-time, actionable information that may help surgeons and health systems to characterize public interest in TKA and THA and to best identify and address patient needs. Relevance for Patients: The GT tool can be used to measure public interest in TKA/THA, which can inform physician expectations for the patient encounter and lead to the creation of decision aids that better inform the public about the risks and benefits of TKA/THA.
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- 2021
12. Public Interest in Elective Orthopedic Surgery Following Recommendations During COVID-19: A Google Trends Analysis
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Rachel Lefebvre, Landon E Cohen, Milan Stevanovic, Christopher Debbaut, Samuel A. Cohen, Matthew J Parham, and Jonathan D Tijerina
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medicine.medical_specialty ,Anterior cruciate ligament reconstruction ,google trends ,medicine.medical_treatment ,Anterior cruciate ligament ,coronavirus ,Healthcare Technology ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,Hip replacement ,Medicine ,Rotator cuff ,Elective surgery ,business.industry ,General surgery ,General Engineering ,Arthroplasty ,medicine.anatomical_structure ,Orthopedics ,elective surgery ,covid-19 ,Orthopedic surgery ,arthroplasty ,orthopaedic surgery ,business ,Orthopedic Procedures ,030217 neurology & neurosurgery ,public interest - Abstract
Introduction Precautions issued by organizations such as the American Academy of Orthopaedic Surgeons (AAOS) recommending against any elective, or non-essential, surgical procedures have significantly affected healthcare resource utilization by the public during the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. In this study, we demonstrate the value of the Google Trends (GT) interface to characterize and monitor in real-time the response in public interest toward various elective orthopedic procedures. Methods Search volume databases were generated from January 2015 to May 2020 for keywords related to anterior cruciate ligament (ACL) reconstruction, elbow arthroplasty, hip arthroplasty, knee arthroplasty, and rotator cuff repair. To measure the immediate effects on public interest, the percent change was calculated from the AAOS, and Centers for Medicare and Medicaid Services (CMS) defined a 30-day pre-recommendations period to a 30-day post-recommendations period. To measure long-term effects, mean search volumes from January 1, 2015, to February 29, 2020, were compared to mean search volumes from March 1, 2020, to May 21, 2020. Results In the 30-day period following statements by the AAOS and CMS, interest in all search terms except "partial knee arthroplasty" decreased as follows: "ACL reconstruction" (-32.7%); "ACL repair" (-22.6%); "anterior cruciate ligament reconstruction" (-39.8%); "elbow arthroplasty" (-17.2%); "elbow joint replacement" (-15.1%); "total elbow arthroplasty" (-40.0%); "hip arthroplasty" (-23.0%); "hip replacement" (-41.2%); "total hip arthroplasty" (-23.0%); "knee arthroplasty" (-43.0%); "total knee arthroplasty" (-33.3%); "rotator cuff repair" (-34.2%); "rotator cuff surgery" (-50.6%); "shoulder arthroplasty" (-26.7%). Discussion GT data have previously demonstrated utility in characterizing and anticipating shifts in real-world healthcare utilization, making it an invaluable tool for physicians to anticipate and address the emerging needs of our patient population. Our study further illustrates the value of GT in localizing rapidly recovering interest in several of the most common elective orthopedic surgeries, enabling surgeons with up-to-date actionable data to guide the management of practices and healthcare facilities as the US slowly emerges from precautions endorsed during the COVID-19 pandemic.
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- 2021
13. Confirmed effects of candidate variants for milk production, udder health, and udder morphology in dairy cattle
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Mekki Boussaha, Marie Pierre Sanchez, Sébastien Fritz, Pascal Croiseau, Anne Barbat, Didier Boichard, Rachel Lefebvre, Chris Hozé, Thierry Tribout, Génétique Animale et Biologie Intégrative (GABI), Université Paris-Saclay-AgroParisTech-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Allice, APIS-GENE, INRAE under the BIG project, ANR-05-GANI-0007,Cartofine,Cartographie fine de régions QTL des ruminants(2005), ANR-08-GENM-0034,LactoScan,Phénotypage et génotupage pour la détection et l'utilisation de QTL influençant significativement la composition fine des laits bovins et ovins(2008), ANR-08-GENM-0030,AMASGEN,Approches Méthodologiques et Applications de la Sélection GENomique chez les bovins laitiers(2008), Services déconcentrés d'appui à la recherche Ile-de-France-Jouy-en-Josas-Antony (SDAR IdF-Jouy-Antony), and Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
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Candidate gene ,lcsh:QH426-470 ,Quantitative Trait Loci ,Single-nucleotide polymorphism ,Genome-wide association study ,Biology ,Quantitative trait locus ,03 medical and health sciences ,Mammary Glands, Animal ,Quantitative Trait, Heritable ,Genetic variation ,Genetics ,medicine ,Animals ,Lactation ,Udder ,Ecology, Evolution, Behavior and Systematics ,Dairy cattle ,lcsh:SF1-1100 ,030304 developmental biology ,Genetic association ,2. Zero hunger ,0303 health sciences ,Polymorphism, Genetic ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,General Medicine ,040201 dairy & animal science ,lcsh:Genetics ,[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics ,Milk ,medicine.anatomical_structure ,Cattle ,Female ,Animal Science and Zoology ,lcsh:Animal culture - Abstract
Background Over the last years, genome-wide association studies (GWAS) based on imputed whole-genome sequences (WGS) have been used to detect quantitative trait loci (QTL) and highlight candidate genes for important traits. However, in general this approach does not allow to validate the effects of candidate mutations or determine if they are truly causative for the trait(s) in question. To address these questions, we applied a two-step, within-breed GWAS approach on 15 traits (5 linked with milk production, 2 with udder health, and 8 with udder morphology) in Montbéliarde (MON), Normande (NOR), and Holstein (HOL) cattle. We detected the most-promising candidate variants (CV) using imputed WGS of 2515 MON, 2203 NOR, and 6321 HOL bulls, and validated their effects in three younger populations of 23,926 MON, 9400 NOR, and 51,977 HOL cows. Results Bull sequence-based GWAS detected 84 QTL: 13, 10, and 30 for milk production traits; 3, 0, and 2 for somatic cell score (SCS); and 8, 2 and 16 for udder morphology traits, in MON, NOR, and HOL respectively. Five genomic regions with effects on milk production traits were shared among the three breeds whereas six (2 for production and 4 for udder morphology and health traits) had effects in two breeds. In 80 of these QTL, 855 CV were highlighted based on the significance of their effects and functional annotation. The subsequent GWAS on MON, NOR, and HOL cows validated 8, 9, and 23 QTL for production traits; 0, 0, and 1 for SCS; and 4, 1, and 8 for udder morphology traits, respectively. In 47 of the 54 confirmed QTL, the CV identified in bulls had more significant effects than single nucleotide polymorphisms (SNPs) from the standard 50K chip. The best CV for each validated QTL was located in a gene that was functionally related to production (36 QTL) or udder (9 QTL) traits. Conclusions Using this two-step GWAS approach, we identified and validated 54 QTL that included CV mostly located within functional candidate genes and explained up to 6.3% (udder traits) and 37% (production traits) of the genetic variance of economically important dairy traits. These CV are now included in the chip used to evaluate French dairy cattle and can be integrated into routine genomic evaluation.
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- 2020
14. Treatment of Iatrogenic Saphenous Neuroma after Knee Arthroscopy with Excision and Allograft Reconstruction
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Mathew Schur, Rachel Lefebvre, Milan Stevanovic, and Kristen M Sochol
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medicine.medical_specialty ,Knee arthroscopy ,Nerve allograft ,medicine.diagnostic_test ,business.industry ,medicine.medical_treatment ,Arthroscopy ,lcsh:Surgery ,lcsh:RD1-811 ,030230 surgery ,Neuroma ,medicine.disease ,Single surgeon ,Surgery ,03 medical and health sciences ,0302 clinical medicine ,Early results ,Peripheral nerve ,030220 oncology & carcinogenesis ,medicine ,Original Article ,business ,Reduction (orthopedic surgery) ,Hand/Peripheral Nerve - Abstract
Background:. The treatment of postoperative, painful sensory neuromas is an ongoing challenge for surgeons. Here, we describe a technique for treatment with excision and allograft reconstruction and report on early results of its use in treating painful saphenous neuromas after knee arthroscopy. Methods:. A retrospective review of a single surgeon’s peripheral nerve clinic from January 1, 2013, to December 31, 2019, was conducted to identify post-knee arthroscopy saphenous neuroma cases in which reconstruction with processed human nerve allograft distally implanted into healthy muscle belly was performed. We examined the outcomes for each patient, including subjective pain self-assessment and need for further surgical treatment. Results:. In total, 9 cases were identified, with patient ages ranging from 21 to 74 years. The average time to referral to peripheral nerve clinic was 31 months (range: 4–143 months). Upon exploration, all nerves were found to have a neuroma in continuity. Six of the 9 patients reported subjective improvement through final follow-up. Three of the 9 patients reported initial improvement, with recurrence of pain at/near the site of the neuroma. The average follow-up time was 9 months (range: 1–21 months). Conclusions:. Here, we report on a novel technique of using a processed human nerve allograft after neuroma resection to provide an organized environment for bridging regenerated axons into muscle tissue. We also describe our early results using this technique to treat iatrogenic saphenous neuromas after knee arthroscopy. Results are encouraging, with 6 of the 9 patients experiencing subjective reduction in pain at final follow-up.
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- 2020
15. Management of Critical Bone Defects
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Geoffrey S, Marecek, Milton T, Little, Michael J, Gardner, Milan, Stevanovic, Rachel, Lefebvre, and Mitchell, Bernstein
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Wound Healing ,Bone Transplantation ,Osteogenesis, Distraction ,Humans ,Musculoskeletal Abnormalities - Abstract
Bone defects may occur after trauma, infection, or oncologic resection. A critical sized defect is any defect that is unable to spontaneously heal and will require secondary procedure(s) to obtain union. Autologous grafting is widely used, but may be insufficient to obtain union in these situations. Other options include the induced membrane technique, bone transport through distraction osteogenesis, or free vascularized bone transfer. This chapter will review options for obtaining graft, and the aforementioned special techniques for managing these challenging problems.
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- 2020
16. Development of a bovine/ovine cytokine 15-plex assay for immunoprofiling of the cellular response in ruminants
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Jérémy Lesueur, Sarah Barbey, Nathan Cebron, Sarah Walachowski, Rachel Lefebvre, Pierre GERMON, Didier Boichard, Fabien Corbière, Gilles Foucras, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Domaine expérimental animal du Pin (SEA), Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), BOVIMUNE, and Institut National de la Recherche Agronomique (INRA)-Université de Tours
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multiplex ,bovin ,[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health ,cytokine ,[SDV.IMM]Life Sciences [q-bio]/Immunology - Abstract
International audience; Evaluation of the immune status must be assessed in highly controlled conditions to be amenable for reproducibility. Furthermore, whereas the cellular response plays a major role, immunity has been measured for a long time through antibody production. For the purpose of assessing immune cell responsiveness in ruminants, we developed and validated a whole blood assay coupled to a high-throughput multiplex cytokine assay using the Luminex technology. A custom Milliplex assay was developed with MERCKMillipore company for the measurement of 15 bovine/ovine cytokines of both the innate and adaptive immunity, and chemokines. Whole blood samples were collected from 110 dairy cows in an INRA experimental unit, and immediately stimulated during 24 hours with heat-killed bacteria (Escherichia coli, Staphylococcus aureus, and Streptococcus uberis), Toll-like receptor ligands (FSL-1 for TLR2, LPS for TLR4, and Gardiquimod for TLR7-8), or soluble anti-CD3/CD28 monoclonal antibodies. Cytokine secretion was determined and compared with a sample prepared in the same condition but without any stimulant. Using ANOVA and data mining methods (PCA, clustering…), we showed that the protocol is able to detect differences between bacteria, with S. uberis being the most potent to induce a response, compared to S. aureus and E. coli that were distinct but more closely related to each other. Similarly, TLR ligands could be distinguished, and Gardiquimod was significantly different from the MyD88- associated TLR2/4-activating ligands. LPS and E. coli provided very similar response profiles confirming previous data indicating that a large part of the E. coli response is mediated through LPS signaling. A high variability of response was detected amongst cattle suggesting environmental and genetic variations of the cytokine response. Altogether, results indicate that cytokine profiling in cattle is achievable and can be used in further work to delineate more precisely the responsiveness of cattle in various situations, including variability of the genetic background.
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- 2019
17. A missense mutation (p.Tyr452Cys) in the CAD gene compromises reproductive success in French Normande cattle
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Mekki Boussaha, Goutam Sahana, M. Mesbah-Uddin, Aurélien Capitan, Chris Hozé, Pauline Michot, Anne Barbat, Rachel Lefebvre, Sébastien Fritz, Didier Boichard, Department of Molecular Biology and Genetics - Center for Quantitative Genetics and Genomics, Aarhus University [Aarhus], Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Allice, and ANR-Apisgene BOVANO
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Male ,[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,Heterozygote ,biology.animal_breed ,Mutation, Missense ,large-scale genotyping ,Biology ,Breeding ,medicine.disease_cause ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,symbols.namesake ,Genetics ,medicine ,Missense mutation ,Animals ,CAD ,Allele ,Normande cattle ,Alleles ,030304 developmental biology ,2. Zero hunger ,Sanger sequencing ,0303 health sciences ,Mutation ,Deoxyribonucleases ,Reproduction ,Haplotype ,Homozygote ,0402 animal and dairy science ,dairy cattle ,04 agricultural and veterinary sciences ,040201 dairy & animal science ,Penetrance ,SNP genotyping ,Haplotypes ,Fertilization ,symbols ,Animal Science and Zoology ,Cattle ,Female ,embryonic lethal ,Food Science ,homozygous haplotype deficiency - Abstract
International audience; We scanned the genome of 77,815 Normande cattle with different Illumina SNP chips (Illumina Inc., San Diego, CA) to map recessive embryonic lethal mutations using homozygous haplotype deficiency. We detected 2 novel haplotypes on chromosomes 11 and 24 but did not confirm 6 previously reported haplotypes. The one on chromosome 11 showed a marked reduction in conception rates and moderate decrease in nonreturn rate in at-risk versus control mating, supporting late embryonic mortality. After fine mapping and analyzing whole-genome sequences, we prioritized a missense mutation in CAD (g.72399397T>C; p.Tyr452Cys)—a gene encoding a protein (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) essential for de novo pyrimidine biosynthesis—as a candidate causal variant. This transition mutation replaces a tyrosine residue, which is perfectly conserved among living organisms, with a cysteine residue in the carbamoyl-phosphate synthetase 2 domain of the protein. A single animal was confirmed to be homozygous for the mutation based on Sanger sequencing. However, large-scale genotyping of the candidate variant with the Illumina EuroG10k BeadChip revealed an absence of live homozygotes in a panel of 33,323 Normande animals and an absence of carriers in 348,593 animals from 19 other cattle breeds. These results support recessive embryonic lethality with nearly complete penetrance, as was previously reported in CAD mutants in several eukaryote species. The only homozygous cow had extremely poor udder conformation, suggesting a potential role of CAD in udder development, but no effect was detected when comparing daughter yield deviations of 250 heterozygous bulls with that of 2,912 homozygotes for the ancestral allele. Together, our results showed the importance of large-scale screening for homozygous haplotype deficiency with hundreds of thousands of animals, validating results with an independent data set, and considering unexpected live homozygotes, to avoid both false-positive and false-negative discoveries. These discoveries will be used primarily in mating decisions to avoid at-risk mating. In addition, we recommend including CAD in the breeding objectives of Normande cattle.
- Published
- 2018
18. Effects of DGAT1 K232A polymorphism and milking frequency on milk composition and spontaneous lipolysis in dairy cows
- Author
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Jocelyne Guinard-Flament, Elise Vanbergue, Jean-Louis Peyraud, Catherine Hurtaud, Yves Gallard, C. Charton, S. Barbey, Rachel Lefebvre, Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Domaine expérimental animal du Pin (SEA), Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), and Institut National de la Recherche Agronomique (INRA)-AgroParisTech
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0301 basic medicine ,medicine.medical_specialty ,Lipolysis ,[SDV]Life Sciences [q-bio] ,Glyceride ,Biology ,medicine.disease_cause ,Milking ,once-daily milking ,03 medical and health sciences ,Animal science ,Internal medicine ,milk composition ,Genotype ,Genetics ,medicine ,Animals ,Lactation ,[INFO]Computer Science [cs] ,Diacylglycerol O-Acyltransferase ,DGAT1 ,chemistry.chemical_classification ,[SDV.GEN]Life Sciences [q-bio]/Genetics ,Lipoprotein lipase ,Polymorphism, Genetic ,dairy cow ,0402 animal and dairy science ,Environmental factor ,Caseins ,food and beverages ,Fatty acid ,spontaneous lipolysis ,04 agricultural and veterinary sciences ,040201 dairy & animal science ,[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics ,Milk ,030104 developmental biology ,Endocrinology ,Enzyme ,chemistry ,Cattle ,Female ,Animal Science and Zoology ,Food Science - Abstract
International audience; Milk spontaneous lipolysis (SL) of milk triglycerides is induced by the lipoprotein lipase, a milk native enzyme, and leads to an accumulation of partial glycerides and free fatty acids that are responsible for the deterioration of the taste of milk products. The gene coding for diacylglycerol acyltransferase 1 (DGAT1), an enzyme implicated in triglycerides synthesis, has an important polymorphic site at the K232A locus. This gene is well known to modulate milk composition. No data are available on the effects of DGAT1 on SL. Thus, a trial was carried out to evaluate the effects of DGAT1 K232A polymorphism on milk SL upon milking frequency variations [once- (ODM) and twice-daily milking (TDM)]. Twenty-one cows were divided into 3 groups according their DGAT1 K232A genotype: 8 cows had the KK genotype of DGAT1 (KK cows), 8 had the KA genotype (KA cows), and 5 had the AA genotype (AA cows). The trial consisted in 3 successive periods: 3 wk of TDM, 3 of ODM, and 4 of TDM. Samples were collected for fat and protein contents, SL, fatty acid, and protein profiles determinations. The KK cows presented higher fat and protein contents, lower milk production, and higher κ-casein percentage. No significant difference in fatty acid composition was noted between groups. The SL was twice as high for KK cows in TDM situations (1.13 vs. 0.59 and 0.63 mEq/100 g of fat, respectively, for KK, KA, and AA cows during the first period of TDM, and 0.46 vs. 0.25 and 0.21 mEq/100 g of fat, respectively, for KK, KA, and AA during the second period of TDM). The SL remained sharply lower in TDM2 than in TDM1. During ODM, no difference in SL was found between groups and SL remained below 0.2 mEq/100 g of fat. These results demonstrate the existence of a correlation between DGAT1 genotypes and spontaneous lipolysis, in interaction with an environmental factor, milking frequency, although it has not been possible to clarify the causal mechanism at this stage.
- Published
- 2016
19. Characteristics of Iatrogenic Nerve Injury from Orthopedic Surgery Correlate with Time to Subspecialty Presentation
- Author
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Paul Navo, Milan Stevanovic, Franco Russo, and Rachel Lefebvre
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medicine.medical_specialty ,business.industry ,Motor nerve ,Original Articles ,030230 surgery ,Nerve injury ,Subspecialty ,Surgery ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Fracture fixation ,Orthopedic surgery ,medicine ,Presentation (obstetrics) ,medicine.symptom ,business ,Orthopedic Procedures ,Sensory nerve - Abstract
Background: There is no current literature examining iatrogenic nerve injury resulting from orthopedic procedures across subspecialties and anatomic areas. This study uses a single peripheral nerve surgeon’s experience to investigate the variable time to presentation of adult patients with iatrogenic nerve injury after orthopedic surgery. Methods: A retrospective review of patients examined in Peripheral Nerve Clinic (PNC) from January 1, 2012, to April 1, 2018, at a single, private, university hospital was performed. Fifty-eight adult patients met inclusion criteria. Charts were reviewed to determine the index orthopedic procedure, peripheral nerve affected, clinical deficits, patient demographics, and time from injury to PNC presentation. Results: The average patient age was 51.2 years, and the average time to PNC referral was 10.9 months after the procedure that resulted in nerve injury. The orthopedic procedures included fracture fixation (13), joint arthroplasty (10) knee arthroscopy and ligament reconstruction (9), mass excision (9), shoulder arthroscopy (7), irrigation and debridement (2), removal of deep hardware (2), tendon procedures (2), trigger digit release (2), nerve decompression (1), and release of exertional compartment syndrome (1). Time from injury to PNC presentation was substantially shorter for patients with upper extremity versus lower extremity deficits (5.9 months vs 19.8 months; P = 0.0173) and for patients with motor nerve involvement versus those with isolated sensory nerve injury (4.5 months vs 24.3 months; P = 0.0164). Conclusions: Iatrogenic nerve injury is a risk across orthopedic subspecialties. Nerve injuries in the lower extremity and those with isolated sensory deficits have significantly delayed time to subspecialty presentation.
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- 2020
20. Peripheral nerve injury with Nexplanon removal: case report and review of the literature
- Author
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Marianne S. Hom, Rachel Lefebvre, Milan Stevanovic, and Hyuma A. Leland
- Subjects
Contraception complications ,medicine.medical_specialty ,Sural nerve ,Case Report ,lcsh:Gynecology and obstetrics ,Iatrogenic peripheral nerve injury ,03 medical and health sciences ,Neuroma ,0302 clinical medicine ,Peripheral nerve ,medicine ,030212 general & internal medicine ,Ulnar nerve injury ,lcsh:RG1-991 ,General Environmental Science ,030219 obstetrics & reproductive medicine ,business.industry ,Implant migration ,Nerve injury ,medicine.disease ,Surgery ,Peripheral ,Peripheral nerve injury ,General Earth and Planetary Sciences ,medicine.symptom ,business ,Ulnar nerve palsy - Abstract
Background Implantable devices offer convenient, long-acting, and reversible contraception. Injury to the peripheral nerves and blood vessels have been reported as rare complications of implantation and extraction. Case presentation We present a case of ulnar nerve injury in a 21-year-old woman from attempted in-office removal of a deeply implanted Nexplanon® device. The injury resulted in an ulnar nerve palsy requiring surgical exploration, neuroma excision, and sural nerve cable grafting. Conclusions In-office attempts to remove contraceptive implants that are deep or have migrated can cause iatrogenic nerve injury. Devices that are non-palpable, deep, or migrated should be imaged before formal surgical exploration and removal. Any patient with neurologic symptoms after placement or after attempted removal requires prompt diagnosis and referral to a peripheral nerve surgeon.
- Published
- 2018
21. Bridge Plate Failure with Extensor Tendon Injury: A Case Report and Literature Review
- Author
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Jessica Intravia, Rachel Lefebvre, Milan Stevanovic, Lisa Cao, and Alidad Ghiassi
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Dorsum ,030222 orthopedics ,medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Case Report ,General Medicine ,Bone healing ,030230 surgery ,medicine.disease ,musculoskeletal system ,Surgery ,Tendon ,03 medical and health sciences ,Fixation (surgical) ,lcsh:RD701-811 ,0302 clinical medicine ,medicine.anatomical_structure ,lcsh:Orthopedic surgery ,Tendon transfer ,Medicine ,Metallosis ,Extensor Carpi Radialis Longus ,business ,Plate fixation - Abstract
Background. Dorsal spanning plate fixation is an increasingly popular method of fixation for certain distal radius fractures. Published outcomes are encouraging, but complications are still reported. Methods. We present a case report of a 26-year-old woman with spanning plate breakage and extensor pollicis longus (EPL) metallosis, fraying, and near-complete rupture. The patient’s unsuccessful follow-up led to this complication. Metallosis and damage to the extensor indices (EI) and distal extensor carpi radialis longus (ECRL) were intraoperative obstacles for tendon transfer to restore EPL function. Results. Tendon transfer in the setting of bridge plate failure has not yet been described in the literature. There are reports of spanning plate failure indicating that breakage often happens through the plate’s holes and after fracture healing. Conclusions. The compounded complication of plate failure with extensor tendon injury emphasizes the important relationship between the local anatomy, barriers to patient care, and potential problems after spanning plate fixation.
- Published
- 2018
22. Genetic parameters of milk production traits in response to a short once-daily milking period in crossbred Holstein × Normande dairy cows
- Author
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Jocelyne Guinard-Flament, Yves Gallard, H. Larroque, C. Charton, Rachel Lefebvre, Didier Boichard, S. Barbey, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Domaine expérimental animal du Pin (SEA), Institut National de la Recherche Agronomique (INRA), Project Ruminflame from the metaprogram GISA, AGROCAMPUS OUEST, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)
- Subjects
0301 basic medicine ,Time Factors ,Lactose ,Biology ,Breeding ,heritability ,Crossbreed ,Genetic correlation ,first day of once-daily milking ,Milking ,03 medical and health sciences ,chemistry.chemical_compound ,Animal science ,Quantitative Trait, Heritable ,fluids and secretions ,Lactation ,Genetics ,medicine ,Animals ,Crosses, Genetic ,threeweek once-daily milking ,2. Zero hunger ,dairy cow ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,0402 animal and dairy science ,food and beverages ,04 agricultural and veterinary sciences ,Heritability ,Milk production ,Milk Proteins ,040201 dairy & animal science ,genetic correlation ,Dairying ,030104 developmental biology ,medicine.anatomical_structure ,Milk ,Phenotype ,chemistry ,Animal Science and Zoology ,Cattle ,Female ,Once daily ,Food Science - Abstract
International audience; Despite its potential utility for predicting cows' milk yield responses to once-daily milking (ODM), the genetic basis of cow milk trait responses to ODM has been scarcely if ever described in the literature, especially for short ODM periods. This study set out to (1) estimate the genetic determinism of milk yield and composition during a 3-wk ODM period, (2) estimate the genetic determinism of milk yield responses (i.e., milk yield loss upon switching cows to ODM and milk yield recovery upon switching them back to twice-daily milking; TDM), and (3) seek predictors of milk yield responses to ODM, in particular using the first day of ODM. Our trial used 430 crossbred Holstein × Normande cows and comprised 3 successive periods: 1 wk of TDM (control), 3 wk of ODM, and 2 wk of TDM. Implementing ODM for 3 wk reduced milk yield by 27.5% on average, and after resuming TDM cows recovered on average 57% of the milk lost. Heritability estimates in the TDM control period and 3-wk ODM period were, respectively, 0.41 and 0.35 for milk yield, 0.66 and 0.61 for milk fat content, 0.60 and 0.80 for milk protein content, 0.66 and 0.36 for milk lactose content, and 0.20 and 0.15 for milk somatic cell score content. Milk yield and composition during 3-wk ODM and TDM periods were genetically close (within-trait genetic correlations between experimental periods all exceeding 0.80) but were genetically closer within the same milking frequency. Heritabilities of milk yield loss observed upon switching cows to ODM (0.39 and 0.34 for milk yield loss in kg/d and %, respectively) were moderate and similar to milk yield heritabilities. Milk yield recovery (kg/d) upon resuming TDM was a trait of high heritability (0.63). Because they are easy to measure, TDM milk yield and composition and milk yield responses on the first day of ODM were investigated as predictors of milk yield responses to a 3-wk ODM to easily detect animals that are well adapted to ODM. Twice-daily milking milk yield and composition were found to be partly genetically correlated with milk yield responses but not closely enough for practical application. With genetic correlations of 0.98 and 0.96 with 3-wk ODM milk yield losses (in kg/d and %, respectively), milk yield losses on the first day of ODM proved to be more accurate in predicting milk yield responses on longer term ODM than TDM milk yield.
- Published
- 2018
23. Sire effect on early and late embryonic death in French Holstein cattle
- Author
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P. Humblot, B. Grimard, Julie Gatien, Claire Ponsart, Marie-Christine Deloche, Rachel Lefebvre, Dorothee Ledoux, Sébastien Fritz, Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Université Paris Est, École nationale vétérinaire d'Alfort (ENVA), Département Recherche et Développement, Union nationale des coopératives d’élevage et d’insémination animale (UNCEIA), Département Recheche et Développement, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, French National Agency for Research (ANR-GENANIMAL program), Apis Gene GIS, AgroParisTech-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA), and Union Nationale des Coopératives Agricoles d'Elevage et d'Insémination Animale
- Subjects
Male ,medicine.medical_specialty ,Animal breeding ,medicine.medical_treatment ,Cattle Diseases ,Breeding ,Biology ,Insemination ,SF1-1100 ,genetic evaluation ,Animal science ,Estrus ,Pregnancy ,Risk Factors ,Internal medicine ,Lactation ,medicine ,Animals ,Genetic Predisposition to Disease ,embryonic death ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,Insemination, Artificial ,2. Zero hunger ,Estrous cycle ,dairy cow ,Artificial insemination ,Sire ,Genetic Variation ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,Abortion, Veterinary ,medicine.disease ,Breed ,Animal culture ,Milk ,Endocrinology ,medicine.anatomical_structure ,genetic selection ,Fertilization ,Embryo Loss ,Cattle ,Female ,Animal Science and Zoology ,female fertility - Abstract
We investigated the effect of maternal sire on early pregnancy failure (between D0, day of insemination and D90) in their progeny during the first and second lactations (n=3508) in the Holstein breed. The estimated breeding value (EBV) for cow fertility of 12 bulls (reliability⩾0.95) was used to create the following three groups: low, medium and high EBV (EBV from −0.7 to 1 expressed as genetic standard deviation relative to the mean of the breed). In their daughters (93 to 516 per bull), progesterone measurement was carried out on the day of artificial insemination (AI; D0) to check whether the cows were in the follicular phase and on D18 to 25 to assess non-fertilisation-early embryonic mortality (NF-EEM). Late embryonic mortality (LEM) and early foetal death (FD) were determined by ultrasonography on D45 and D90 and by the return to oestrus after the first AI. Frequencies of NF-EEM, LEM, FD and pregnancy were 33.3%, 11.7%, 1.4% and 48.5% and incidences were 35.1, 19.0, 2.7 and 51.1, respectively. Sire EBV was significantly related to the incidences of pregnancy failure between D0 and D90, fertilisation failure-early embryonic mortality (FF-EEM) and LEM but not to the incidence of FD between D45 and D90 of pregnancy. The relative risk (RR) of FF-EEM was significantly higher (RR=1.2; P
- Published
- 2015
24. Genome-wide association study for milking speed in French Holstein cows
- Author
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Sébastien Fritz, Goutam Sahana, Mogens Sandø Lund, Rachel Lefebvre, Anne Barbat, Bernt Guldbrandtsen, Didier Boichard, Andrew Marete, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris Saclay (COmUE), Center for Quantitative Genetics and Genomics, Aarhus University [Aarhus], and Allice
- Subjects
0301 basic medicine ,Genotype ,[SDV]Life Sciences [q-bio] ,Quantitative Trait Loci ,Genome-wide association study ,Single-nucleotide polymorphism ,Quantitative trait locus ,Biology ,mastitis ,Polymorphism, Single Nucleotide ,milking speed ,Milking ,03 medical and health sciences ,pleiotropy ,Genetics ,medicine ,SNP ,Animals ,Genetic association ,2. Zero hunger ,[SDV.GEN]Life Sciences [q-bio]/Genetics ,genome-wide association study ,bovine ,0402 animal and dairy science ,food and beverages ,04 agricultural and veterinary sciences ,medicine.disease ,040201 dairy & animal science ,Mastitis ,[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics ,Dairying ,030104 developmental biology ,Milk ,Phenotype ,Animal Science and Zoology ,Cattle ,Female ,Food Science ,SNP array ,Genome-Wide Association Study - Abstract
Using a combination of data from the BovineSNP50 BeadChip SNP array (Illumina, San Diego, CA) and a EuroGenomics (Amsterdam, the Netherlands) custom single nucleotide polymorphism (SNP) chip with SNP pre-selected from whole genome sequence data, we carried out an association study of milking speed in 32,491 French Holstein dairy cows. Milking speed was measured by a score given by the farmer. Phenotypes were yield deviations as obtained from the French evaluation system. They were analyzed with a linear mixed model for association studies. We identified SNP on 22 chromosomes significantly associated with milking speed. As clinical mastitis and somatic cell score have an unfavorable genetic correlation with milking speed, we tested whether the most significant SNP on these 22 chromosomes associated with milking speed were also associated with clinical mastitis or somatic cell score. Nine hundred seventy-one genome-wide significant SNP were associated with milking speed. Of these, 86 were associated with clinical mastitis and 198 with somatic cell score. The most significant association signals for milking speed were observed on chromosomes 7, 8, 10, 14, and 18. The most significant signal was located on chromosome 14 ( ZFAT gene). Eleven novel milking speed quantitative trait loci (QTL) were observed on chromosomes 7, 10, 11, 14, 18, 25, and 26. Twelve candidate SNP for milking speed mapped directly within genes. Of these 10 were QTL lead SNP, which mapped within the genes HMHA1 , POLR2E , GNB5 , KLHL29 , ZFAT , KCNB2 , CEACAM18 , CCL24 , and LHPP . Limited pleiotropy was observed between milking speed QTL and clinical mastitis.
- Published
- 2017
25. A divergent genetic selection experiment on mastitis resistance in Holstein and Normand breeds
- Author
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Rachel Lefebvre, Sarah Barbey, Frédéric Launay, Didier Boichard, Pierre GERMON, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Domaine expérimental animal du Pin (SEA), Institut National de la Recherche Agronomique (INRA), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Federation International du Lait., and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)
- Subjects
mammites, bovins, sélection ,[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2016
26. Using whole genome sequences to identify QTL for udder health and morphology in French dairy cattle
- Author
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Thierry Tribout, Marine Barbat, Armelle Gion, Amandine Launay, Rachel Lefebvre, Anne Barbat, mekki boussaha, Pascal Croiseau, Marie Pierre Sanchez, Sebastien Fritz, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris Saclay (COmUE), Allice, and Institut de l'élevage (IDELE)
- Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics ,[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics ,[SDV]Life Sciences [q-bio] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2016
27. Identification of candidate causal variants underlying QTL in dairy cattle through GWAS and Bayesian approach at the sequence level
- Author
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Didier Boichard, Marie Pierre Sanchez, Anne Barbat, mekki boussaha, Thierry Tribout, Rachel Lefebvre, Sebastien Fritz, Romain Saintilan, Chris Hoze, Armelle Govignon-Gion, Pascal Croiseau, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Genetics Team, Union nationale des coopératives d’élevage et d’insémination animale (UNCEIA), and ANR - Apisgene PhenoFinLait / Lactoscan et CartoSeq
- Subjects
séquence du génome ,bovin laitier ,analyse d'association ,composition du lait ,séquence du génome complet ,[SDV]Life Sciences [q-bio] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2016
28. Halofuginone Inhibits T H 17 Cell Differentiation by Activating the Amino Acid Starvation Response
- Author
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Hanspeter Waldner, Ava Rhule-Smith, Mark S. Sundrud, Anjana Rao, Markus Feuerer, Dinis Pedro Calado, Sergei B. Koralov, Aimee ElHed Kozhaya, Tracy Keller, Malcolm Whitman, Derya Unutmaz, Ralph Mazitschek, and Rachel Lefebvre
- Subjects
Encephalomyelitis, Autoimmune, Experimental ,Cellular differentiation ,Eukaryotic Initiation Factor-2 ,Gene Expression ,Autoimmunity ,Protein Serine-Threonine Kinases ,Biology ,medicine.disease_cause ,Mice ,Piperidines ,T-Lymphocyte Subsets ,In vivo ,medicine ,Animals ,Humans ,Amino Acids ,Phosphorylation ,Quinazolinones ,chemistry.chemical_classification ,Multidisciplinary ,Halofuginone ,Lymphopoiesis ,Interleukin-17 ,Experimental autoimmune encephalomyelitis ,Cell Differentiation ,T-Lymphocytes, Helper-Inducer ,medicine.disease ,Activating Transcription Factor 4 ,Amino acid ,Cell biology ,Mice, Inbred C57BL ,chemistry ,Biochemistry ,Cytokines ,Signal transduction ,Starvation response ,Signal Transduction ,medicine.drug - Abstract
Starving T Cells The T H 17 lineage of CD4 + helper T cells, characterized by the ability to secrete IL-17, is an important mediator of inflammation and autoimmunity. Dampening the responses of these cells or inhibiting their differentiation is of great therapeutic interest. Sundrud et al. (p. 1334 ; see the Perspective by Blander and Amsen ) now show that the small molecule halofuginone inhibits the differentiation of T H 17 cells but not other CD4 + T cell helper lineages both in vitro and in a mouse model of multiple sclerosis. This selective inhibition was mediated by activation of the amino acid starvation response. Amino acid depletion mimicked the effects of halofuginone, whereas excess amino acids rescued T H 17 differentiation. The results highlight the importance of amino acid metabolism in regulating inflammation.
- Published
- 2009
29. Effectiveness of Risperidone in Comorbid Bipolar Disorder and Substance Abuse: An Observational Study
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Rachel Lefebvre, Jesse J. Suh, Reynolds C. Clodfelter, S. Nassir Ghaemi, and Mark J. Albanese
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Pediatrics ,medicine.medical_specialty ,Risperidone ,Addiction ,media_common.quotation_subject ,Dopamine antagonist ,medicine.disease ,Substance abuse ,Psychiatry and Mental health ,medicine ,Observational study ,Bipolar disorder ,Substance use ,Substance abuse treatment ,Psychology ,Psychiatry ,media_common ,medicine.drug - Abstract
This report will describe clinical experience using risperidone in substance-dependent patients with bipolar disorder. Method. Thirty-six male patients with SUD and bipolar disorder (DSMIII-R), who were admitted to a voluntary, post-detoxification, intermediatecare inpatient substance abuse program, were assessed weekly using the Clinical Global Impressions scale to assess overall functioning. All patients were treated with risperidone (mean dose 2.3 mg/day) in an open-label, naturalistic trial. 20 Results. Of the 36 patients, 11 dropped out before a follow-up appointment. Twenty patients (56%) reported improved or much improved on the CGI scale, and 5 (%) were rated unchanged. Conclusion. The results of this observational trial suggest that risperidone is moderately effective and well tolerated in bipolar patients with substance use disorders and comorbid psychiatric illnesses. In the short-term, risperidone treatment may also contribute to the likelihood of completing substance abuse treatment.
- Published
- 2008
30. Dairy cattle reproduction is a tightly regulated genetic process: Highlights on genes, pathways, and biological processes
- Author
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Caroline Eozenou, Damien Valour, Svetlana Uzbekova, Laurent Schibler, Claire Ponsart, Eli Sellem, Rachel Lefebvre, Aurelie Bonnet, Pauline Michot, Aurélien Capitan, Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Union nationale des coopératives d’élevage et d’insémination animale (UNCEIA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Santé Animale, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), REPROSEQ project funded by APIS-GENE, Institut National de la Recherche Agronomique (INRA), Union Nationale des Coopératives Agricoles d'Elevage et d'Insémination Animale, Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), AgroParisTech-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,media_common.quotation_subject ,Fertility ,Computational biology ,Culling ,Biology ,Genome ,03 medical and health sciences ,0302 clinical medicine ,Food Animals ,environmental factor ,Dairy cattle ,030304 developmental biology ,media_common ,2. Zero hunger ,0303 health sciences ,030219 obstetrics & reproductive medicine ,business.industry ,Modelling biological systems ,genetic factor ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,Omics ,reproductive performance ,Biotechnology ,omics ,Agriculture ,Sustainability ,Animal Science and Zoology ,business - Abstract
Herd fertility is a key factor for the sustainability of cattle farming. However, declining fertility has become one of the main causes of culling and replacement of cows in developed countries. Omics technologies have been successfully developed in cattle, refining our knowledge of molecular mechanisms governing cattle reproduction. However, most studies have failed to establish clear links between the genome and final phenotypes. Further studies will be needed to integrate all Omics data and develop a systems biology modeling approach to tackle this complex biological function.
- Published
- 2015
31. Performances zootechniques et aptitude fromagère en 1ère lactation de vaches croisées Holstein x Normande sélectionnées pour le gène DGAT1
- Author
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Sarah Barbey, Rachel Lefebvre, Emeline Desplaces, AGNES DELACROIX-BUCHET, Didier Boichard, Domaine expérimental animal du Pin (SEA), Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), and AgroParisTech-Institut National de la Recherche Agronomique (INRA)
- Subjects
Bovin laitier ,Gène DGAT1 ,[SDV]Life Sciences [q-bio] - Abstract
absent
- Published
- 2014
32. Developing a baseline assessment battery: balancing patient time burden with essential clinical and research monitoring
- Author
-
David R. Gastfriend, Dennis M. Donovan, Rachel Lefebvre, and Kelly T Murray
- Subjects
medicine.medical_specialty ,Psychometrics ,Psychological intervention ,MEDLINE ,Physical examination ,Cost of Illness ,Drug Therapy ,Behavior Therapy ,Surveys and Questionnaires ,Humans ,Medicine ,Combined Modality Therapy ,Psychiatry ,Intensive care medicine ,Baseline (configuration management) ,Clinical Trials as Topic ,Modalities ,medicine.diagnostic_test ,business.industry ,Research ,General Medicine ,Alcoholism ,Clinical research ,Feasibility Studies ,Clinical Trials Data Monitoring Committees ,business ,Reinforcement, Psychology - Abstract
Baseline assessment in a multisite alcohol-dependence treatment study has several purposes: addressing inclusion/exclusion criteria and characterizing participants to illuminate subsequent efficacy and safety patterns of the interventions. Combination pharmacotherapy and behavioral therapy trials, however, require more complex assessments than single-modality studies. Medication trials require measures of initial safety for study drug as well as for subsequent side-effect and adverse-effect monitoring (e.g., physical examination, laboratory markers, somatic symptoms, medical conditions and concomitant medications). Behavioral therapy trials (and in some cases medication trials) warrant baseline measurement of mediators of therapy effect (e.g., prior treatment history, motivation for change, self-efficacy, other psychiatric conditions, treatment expectations and network supports). Measures may be needed to interpret interactions that may be discovered between these modalities.The National Institute on Alcohol Abuse and Alcoholism COMBINE Study evaluated the potentially overwhelming number of candidate instruments through an iterative process, using the following sequence to finalize a rational baseline assessment battery: key constructs, representative measures, determination of duration of assessment, risk of assessment reactivity, goal priorities, necessity for measure "pruning" and order of measure presentation. After selecting the draft battery, feasibility was evaluated in a pilot study prior to the main trial.The battery was feasible to administer and avoided unintended selection bias. Dropout was substantial, however, and differences across sites in baseline assessment completion rates reflected a tendency of a central intake model to function as an initial filter of dropouts, compared with a direct recruitment model.This process holds several potentially useful lessons for investigators.
- Published
- 2005
33. Perceptions des relations mère-enfant dans les familles d’enfants présentant un état limite
- Author
-
Jean-Marc Guilé, Nina Howe, and Rachel Lefebvre
- Abstract
L’objectif de cet article est d’examiner les relations mere-enfant dans les familles d’enfants presentant un etat limite (EL). Vingt-deux meres ainsi que leur enfant presentant un trouble limite ont ete interroges simultanement a propos de la qualite et du type de leurs interactions. Les profils de perceptions rapportes ont ete compares a ceux de vingt-deux meres et de leur enfant qui ne presentait pas de trouble emotionnel. Des donnees portant, d’une part, sur les perceptions qu’ont les meres de leur style parental ainsi que, d’autre part, sur les perception qu’ont les enfants du style parental de leur mere ont ete recueillies. Les meres d’enfants EL ont dit utiliser plus de controle par la culpabilite et se sont dites plus centrees sur leur enfant que les meres du groupe temoin. Cependant, le fait qu’elles se disent plus centrees sur leur enfant a ete interprete comme etant de la surprotection etant donne la nature des variables composant l’echelle mesurant ce phenomene. Les enfants EL ont rapporte moins d’affection, plus d’agression, plus de negligence et de rejet ainsi que plus de controle de la part de leur mere que les enfants du groupe temoin. Les enfants EL ont rapporte des types d’interactions avec leur mere qui sont caracterises par du controle sans affection. Ces memes types d’interactions ont ete observes chez des adultes EL dans des etudes ulterieures.
- Published
- 2004
34. The Same Old Song?—Stability and Change in Relationship Schemas from Adolescence to Young Adulthood
- Author
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Rachel Lefebvre, Joseph P. Allen, Robert J. Waldinger, Frank Guastella, Louis Diguer, Stuart T. Hauser, and Lester Luborsky
- Subjects
Longitudinal study ,Social Psychology ,Social perception ,Adult development ,media_common.quotation_subject ,education ,Interpersonal communication ,Article ,Education ,Developmental psychology ,Interpersonal relationship ,Schema (psychology) ,Developmental and Educational Psychology ,Personality ,Young adult ,Psychology ,Social Sciences (miscellaneous) ,media_common - Abstract
Relationship schemas are core elements of personality that guide interpersonal functioning. The aim of this study is to examine stability and change in relationship schemas across two developmental epochs-adolescence and young adulthood-in the stories that people tell about their interactions with others. Using the Core Conflictual Relationship Theme Method, relationship themes were coded from semistructured interviews conducted in adolescence and again at age 25. The sample consisted of 40 participants in a longitudinal study of adolescent and young adult psychological development. There was considerable stability in the frequency with which particular themes were expressed in the narratives of adolescents and young adults. Significant changes from adolescence to young adulthood included a decrease in the perception of others as rejecting and of the self as opposing others. Young adults saw themselves and others more positively, and used a broader repertoire of themes in their relationship narratives than they had as adolescents. The basic continuity and particular changes in relationship schemas found in this study are consistent with knowledge about the adolescent-to-young-adult transition derived from other empirical and clinical findings. Relationship schemas may be rich units of study for learning about the development of interpersonal functioning.
- Published
- 2002
35. Détection de QTL de caractères originaux dans une population bovine croisée Holstein x Normande
- Author
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Rachel Lefebvre, Helene Larroque, Sarah Barbey, Yves Gallard, Jacques Colleau, J., Céline Chantry-Darmon, AGNES DELACROIX-BUCHET, Anne-Lyse Laine, Didier Boichard, Génétique Animale et Biologie Intégrative (GABI), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Domaine expérimental animal du Pin (SEA), Institut National de la Recherche Agronomique (INRA), Laboratoire d'Analyse Génétique pour les Espèces Animales (LABOGENA), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut de l'Elevage (IDELE). Paris, FRA., and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences ,Bovin laitier ,QTL ,croisement - Abstract
Un protocole de détection de QTL en croisement entre les races Prim'Holstein et Normande a été mené à l'Unité Expérimentale INRA du Pin-au-Haras. Ce programme a pour objectif de détecter et cartographier des QTL responsables de la variabilité intra et entre races. 866 femelles F2 ont été suivies jusqu'en première lactation, ainsi que 324 femelles " F3 " issues d'un père F1 et d'une mère F2. Les phénotypes mesurés concernent les caractères classiquement mesurés en ferme, mais surtout un grand nombre de caractères originaux. Les résultats présentés concernent l'aptitude fromagère des laits individuels, la précocité sexuelle, la reprise de cyclicité post-partum, et la coloration de la robe. Tous les individus F0 à F3 sont génotypés sur la puce bovine 50k d'Illumina. Les détections ont été faites par analyse de liaison et déséquilibre de liaison. L'âge à la puberté est en moyenne de 307 jours (± 51) et son héritabilité est estimée à 0,37. Des QTL sont détectés sur 13 chromosomes (1, 2, 3, 5, 8, 11, 12, 13, 14, 21, 25, 27 et 29), les plus forts étant sur les chromosomes 29 (pic à 26 Mb) et 13 (61 Mb). Le délai de reprise de cyclicité est en moyenne de 31 jours (± 12) et son héritabilité est estimée à 0,17. 14 QTL sont détectés sur 9 chromosomes (1, 3, 5, 8, 11, 17, 21, 26 et 27) avec les plus forts sur les chromosomes 21 (26 Mb) et 26 (33 Mb). Seuls 2 QTL sont communs aux deux caractères, sur le chromosome 21, reflétant leur faible corrélation génétique (0,24). L'aptitude fromagère dépend très fortement du génotype au locus des caséines, ainsi que de la beta-lactoglobuline et de DGAT1. Après prise en compte de ces locus, 5 à 8 QTL sont détectés pour les différents caractères analysés (temps de prise, fermeté du gel, rendement, gras sur sec). Ils sont en général différents entre caractères et ont des effets plus réduits. Concernant le patron de coloration, MC1R est responsable de la couleur, ASIP de la bringeure, MITF de la tête blanche, du pourcentage de blanc, de la couleur des onglons, KIT du pourcentage de blanc et du type de panachure. Une nouvelle région sur le chromosome 8 est mise en évidence et affecte le pourcentage de blanc et le type de panachure.
- Published
- 2012
36. Detection of QTL controlling cheese processing properties in a Holstein x Normande crossbred population
- Author
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Xue Yong, Helene Larroque, Sarah Barbey, Rachel Lefebvre, Yves Gallard, Jean-Claude Ogier, Jacques Colleau, J., AGNES DELACROIX-BUCHET, Didier Boichard, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Domaine expérimental animal du Pin (SEA), Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Institut National de la Recherche Agronomique (INRA), and Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA)
- Subjects
CHEESE ,QTL ,CHROMOSOME ,[SDV]Life Sciences [q-bio] ,PROTEIN ,HOLSTEIN ,NORMANDE ,BOVIN ,MILK ,GENOTYPE ,LINKAGE ,DISEQUILIBRIUM ,BETA-LACTOGLOBULIN - Abstract
absent
- Published
- 2011
37. 'This is a bad dog, you know...': constructing shared meanings during sibling pretend play
- Author
-
Hariclia Harriet Petrakos, Nina Howe, Rachel Lefebvre, and Christina M. Rinaldi
- Subjects
Male ,Firstborn ,Social understanding ,Concept Formation ,Context (language use) ,Fantasy ,Language Development ,Vocabulary ,Education ,Developmental psychology ,Developmental and Educational Psychology ,State language ,Humans ,Sibling Relations ,Sibling ,Child ,Negotiating ,Age Factors ,Sibling relationship ,Play and Playthings ,Semantics ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Imagination ,Female ,Birth Order ,Construct (philosophy) ,Psychology ,Comprehension ,Social psychology ,Meaning (linguistics) - Abstract
The construction of shared meanings in play, pretense enactment, internal state language, and sibling relationship quality were investigated in 40 kindergarteners with an older ( M age=7.10 years) or younger ( M age=3.6 years) sibling. Dyadic strategies to construct shared meanings (e.g., extensions, building on) were positively associated with frequency of pretense and internal state language. Developmental differences indicated that older dyads used more shared meaning strategies, whereas younger dyads engaged in nonmaintenance behaviors (i.e., disruptions to flow of play). Furthermore, firstborn kindergarteners used more nonmaintenance behaviors, whereas second-born kindergarteners extended partner's ideas. Findings highlight the sibling relationship as a context for illuminating social understanding and relationship dynamics during pretend play. [ABSTRACT FROM AUTHOR]
- Published
- 2005
38. An Unusual Case of Refractory Celiac Disease
- Author
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Rachel Lefebvre and Neil Yager
- Subjects
medicine.medical_specialty ,Unusual case ,Hepatology ,Refractory ,business.industry ,Gastroenterology ,Medicine ,Disease ,business ,Dermatology - Published
- 2011
39. Réponse à une sélection divergente sur la résistance aux mammites et la mobilisation corporelle en races Holstein et Normande
- Author
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Rachel Lefebvre, Sarah Barbey, Frédéric Launay, Pierre GERMON, Didier Boichard, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Domaine expérimental animal du Pin (SEA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)
- Subjects
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
40. Identification and characterization of microRNA genetic variants in dairy cattle, from their detection to the analysis of their biological impacts
- Author
-
Céline Bourdon, Mekki Boussaha, Marie-Pierre Sanchez, Thierry Tribout, Rachel Lefebvre, Sandrine Le Guillou, Gwenola Tosser-Klopp, Fabienne Le Provost, and TOSSER-KLOPP, GWENOLA
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV.BA] Life Sciences [q-bio]/Animal biology ,[SDV.GEN] Life Sciences [q-bio]/Genetics
41. Identification de QTL associés à de la mortalité embryonnaire chez les bovins laitiers
- Author
-
Sébastien Fritz, Aurelien Capitan, Anis Djari, Cecile Grohs, mekki boussaha, Aurélia Baur, Anne Barbat, Rachel Lefebvre, Diane Esquerre, Christophe Klopp, Dominique Rocha, Didier Boichard, Union nationale des coopératives d’élevage et d’insémination animale (UNCEIA), Unité de Biométrie et Intelligence Artificielle (UBIA), Institut National de la Recherche Agronomique (INRA), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, ANR Cartofine, Union Nationale des Coopératives Agricoles d'Elevage et d'Insémination Animale, Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), and AgroParisTech-Institut National de la Recherche Agronomique (INRA)
- Subjects
fertilité ,bovin ,qtl ,[SDV]Life Sciences [q-bio] ,mortalité embryonnaire - Abstract
Des régions du génome portant des mutations récessives délétères ont été détectées dans les trois principales races laitières françaises en recherchant des haplotypes relativement fréquents (>1%) dans la population mais présentant un fort déficit en homozygotes. La détection repose sur l’utilisation des données de typages de la puce BovineSNP50 ® d’Illumina utilisées en sélection génomique (47 878 animaux Holstein, 16 833 Montbéliards, et 11 466 Normands). Trente trois régions incluant les régions déjà décrites associées à Brachyspina, CVM, HH1 et HH3 en race Holstein ont été identifiées. La longueur de ces régions varie de 1 à 4,8 Mb et les fréquences des haplotypes de 1,8 à 9%. Un effet significatif négatif sur la fertilité a été observé pour 10 de ces régions dans les accouplements à risque entre taureaux porteurs et filles de taureaux porteurs, confortant leur association avec des mutations létales à l’état embryonnaire. Des effets significatifs ont également été observés sur la mortinatalité pour 7 haplotypes. Ces régions ont été ensuite analysées avec des données de séquence complète du génome de taureaux porteurs et contrôle (45 animaux au total). Des mutations candidates très fortes ont été mises en évidence, dont trois déjà connues dans les gènes FANCI (Brachyspina), SLC35A3 (CVM), et APAF1 (HH1) et quatre nouvelles mutations avec des effets prédits très délétères sur la structure protéique dans les gènes GART, SMC2, SLC37A2, et NOA1. En conclusion, cette étude montre un aspect jusqu’à présent inconnu de la consanguinité dans les races bovines sélectionnées. Alors qu’une éradication systématique serait extrêmement coûteuse financièrement pour les programmes de sélection, une contre-sélection doit être entreprise pour diminuer progressivement la fréquence de ces mutations. Les plans d’accouplement doivent dès à présent éviter les accouplements à risque., Genomic regions harboring recessive deleterious mutations were detected in three dairy cattle breeds by identifying frequent haplotypes (>1%) showing a deficit in homozygotes among Illumina BovineSNP50 ® Beadchip haplotypic data from the French genomic selection database (47,878 Holstein, 16,833 Montbéliarde, and 11,466 Normande animals). Thirty-three candidate haplotypes were identified including previously reported regions associated with Brachyspina, CVM, HH1, and HH3 in the Holstein breed. Haplotype length varied from 1 to 4.8 Mb and frequencies from 1.8 up to 9%. A significant negative effect on calving rate was observed for 10 of these haplotypes in matings between carrier bulls and daughters of carrier sires, confirming their association with embryonic lethal mutations. Significant effects were also detected on stillbirth for 7 haplotypes. These regions were further investigated using whole genome sequencing data from heterozygous bull carriers and control animals (45 animals in total). Seven strong candidate causative mutations including polymorphisms previously reported in FANCI (Brachyspina), SLC35A3 (CVM), APAF1 (HH1) and four novel mutations with very damaging predicted effect on the protein structure were detected in GART, SMC2, SLC37A2, and NOA1 genes. In conclusion, this study reveals a yet hidden consequence of the important inbreeding rate observed in intensively selected and specialized cattle breeds. Whereas a systematic eradication would be extremely costly and damaging for the breeding scheme, a gradual counter selection must be undertaken to decrease their frequency and an appropriate mating plan must be carried out to avoid matings at risk.
42. Sélection divergente sur la résistance aux mammites en races Holstein et Normande : comparaison des réponses génétique et immunitaire entre lignées
- Author
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Rachel Lefebvre, Sarah Barbey, Frédéric Launay, Pascal Rainard, Gilles Foucras, Didier Boichard, Pierre GERMON, Génétique Animale et Biologie Intégrative (GABI), Université Paris-Saclay-AgroParisTech-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Domaine expérimental animal du Pin (SEA), Institut National de la Recherche Agronomique (INRA), Infectiologie et Santé Publique (UMR ISP), Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
- Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics ,[SDV]Life Sciences [q-bio] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
43. French genomic experience: genomics for all ruminant species
- Author
-
Eric Venot, Anne Barbat, Didier Boichard, Pascal Croiseau, Vincent Ducrocq, Rachel Lefebvre, Florence Phocas, Marie Pierre Sanchez, Thierry Tribout, Aurélie Vinet, Marie-Noelle Fouilloux, Armelle Govignon Gion, Amandine Launay, Julie Promp, Marine Barbat, Aurélia Baur, Chris Hoze, Sebastien Fritz, Romain Saintilan, Céline Carillier, Helene Larroque, Andres Legarra, Isabelle Palhiere, Céline Robert Granié, Rachel Rupp, Flavie Tortereau, Astruc, J. M., Clément, V., Loywyck, V., Boulesteix, P., Sophie Mattalia, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris Saclay (COmUE), Institut de l'élevage (IDELE), Allice, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, and Université Fédérale Toulouse Midi-Pyrénées
- Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics ,[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics ,ruminants ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,sélection génomique ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
44. Effects of diacylglycerol-O-transferase 1 (DGAT1) gene polymorphism and milking frequency on cow milk spontaneous lipolysis
- Author
-
Elise Vanbergue, Jocelyne Guinard-Flament, Sarah Barbey, Clémentine Charton, Rachel Lefebvre, Yves Gallard, Catherine Hurtaud, Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut de l'élevage (IDELE), Domaine expérimental animal du Pin (SEA), Institut National de la Recherche Agronomique (INRA), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, and ProdInra, Migration
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] - Abstract
International audience; La lipolyse spontanée (LS) du lait dépend de nombreux facteurs d’élevage, dont la fréquence de traite, et la génétique de l’animal. Nous avons donc testé l’effet du gène DGAT1, polymorphique au locus K232A, sur la LS dans un contexte de variation de la fréquence de traite. Huit vaches « KK », 8 vaches « KA » et 5 vaches « AA » ont été utilisées. L’essai s’est déroulé sur 3 périodes successives : une semaine de référence en bitraite, 3 semaines de monotraite et une semaine de bitraite. Les prélèvements ont été effectués à J-4, J0 (date de passage en monotraite), J1, J2, J7 et J24, pour évaluer le taux butyreux (TB), le taux protéique (TP), la composition en acides gras (AG) et en protéines, et la LS. Les vaches « KK » ont présenté un TB et un TP plus élevés, une production laitière plus faible, un pourcentage de caséines ? plus élevé que les vaches « AA ». Les profils en AG n’ont pas présenté de différence. En bitraite, la LS a été deux fois plus élevée chez les vaches « KK » que chez les vaches « KA » et « AA » (1,28 vs 0,59 et 0,63 mEq/100g de MG respectivement, pendant la période de référence).En monotraite, la LS a été identique dans les 3 groupes et inférieure à 0,2 mEq/100 g de MG. L’existence d’un déterminisme génétique de la lipolyse, confirmée par une étude à plus grande échelle, pourrait être prise en compte lors de la sélection génétique des animaux.
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