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The aim of the study was to assess the effects of therapeutic ultrasound (US) on oxidative stress (OS)-induced changes in cultured human chondrocytes (HCH). For this, monolayer HCH were randomized in three groups: a control group (CG), a group exposed to OS (OS group), and a group exposed to US and OS (US-OS group). US exposure of the chondrocytes was performed prior to OS induction by hydrogen peroxide. Transmission electron microscopy (TEM) was used to assess the chondrocytes ultrastructure. OS and inflammatory markers were recorded. Malondialdehyde (MDA) and tumor necrosis factor (TNF)-α were significantly higher (p < 0.05) in the OS group than in CG. In the US-OS group MDA and TNF-α were significantly lower (p < 0.05) than in the OS group. Finally, in the US-OS group MDA and TNF-α were lower than in CG, but without statistical significance. TEM showed normal chondrocytes in CG. In the OS group TEM showed necrotic chondrocytes and chondrocytes with a high degree of vacuolation and cell organelles damages. In the US-OS group the chondrocytes ultrastructure was well preserved, and autophagosomes were generated. In conclusion, US could protect chondrocytes from biochemical (lipid peroxidation, inflammatory markers synthesis) and ultrastructural changes induced by OS and could stimulate autophagosomes development.

Antioxidants have been commonly used in medicine for thousands of years. Clinically, pentoxifylline and Ginkgo biloba have beneficial renal effects. Our study evaluated the nephroprotective effect of Gingko biloba in combination with Pentoxifiln in an experimental model of vancomycin-induced nephrotoxicity. Male Winstar rats were used in 3 groups: CONTROL, VANCO and VANCO+GBI+PTX and each group included 6 rats. Insufficient studies in the literature on the prevention of acute kidney injury by the combination of Ginkgo biloba and pentoxifylline led to the necessity to perform the study. Acute kidney injury was demonstrated by measuring serum values of classical markers such as urea and creatinine but also by measuring the urinary N-acetyl-β-d-glucosaminidase index, a topical marker in modern medicine. The significant decrease of the biochemical parameters in group III (VANCO+GBI+PTX) compared to group II (VANCO) and values similar to group I (CONTROL), demonstrates, the nephroprotective effect of the use in combination of the two substances.

Donkey milk is used as an alternative source in the diet of young children and newborns due to the similar composition to breast milk. The donkey milk is considered to be a functional food by the chemical composition it presents, thus being beneficial in the nutrition of people suffering from food allergies. The chemical composition and antioxidant capacity of the donkey milk are significantly influenced by lactation and also by the lactation period. Donkey milk has a low fat content compared to other species and high lactose content. These parameters are influenced by the variables such as: lactation, animal age, and feeding. The purpose of this study was to determine the effect of lactation on the antioxidant capacity and physicochemical compounds in the donkey milk., donkey lactation of milk

Mare's milk is valuable because of its nutritional properties. It is used in human food and tartamente for various diseases. Mare milk changes its composition depending on the lactation. It has the lowest values in first and second lactation in lactating 3, the highest values. The fat content varies between 1.88 g/100 g (lactation 1) and 2.17 g/100 g (lactation 3). Milk protein varies between 1.74 g/100g (lactation 1) and 1.92 g/100 g (lactation 3). For colostrum, affecting all day postpartum physico-chemical parameters. The highest values for these parameters are within 3 days postpartum. On day 5, the low values are observed. The fat content of the colostrum varies between 2.85 g/100 g, on day 1, and 2.13 g/100 g, on day 5.