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The effects of the four heating intensities (hot-spring egg yolk, HEY; soft-boiled egg yolk, SEY; normal-boiled egg yolk, NEY; and over-boiled egg yolk, OEY) on lipidomes of boiled egg yolks were investigated. The results indicated that four heating intensities had no significant effect on the total abundance of lipids and lipid categories except for bile acids, lysophosphatidylinositol, and lysophosphatidylcholine. However, of all the 767 lipids quantified, the differential abundance of 190 lipids was screened among the egg yolk samples at four heating intensities. Soft-boiling and over-boiling altered the assembly structure of the lipoproteins through thermal denaturation and affected the binding of lipids and apoproteins, resulting in an increase in low-to-medium-abundance triglycerides. The decreased phospholipid and increased lysophospholipid and free fatty acid in HEY and SEY suggests potential hydrolysis of phospholipids under relatively low-intensity heating. Results provide new insights into the effect of heating on the lipid profiles of egg yolk and would support the public’s choice of cooking method for egg yolks.

The study was implemented for exploring influences of nano-K

The Procapra przewalskii inhabits in a selenium (Se)-deprived environment in long-term, but they have no pathological manifestations due to the Se deprivation. This study aimed to reveal the underlying adaptation induced by Se deprivation. In the analysis, a total of 93 significantly changed metabolites were identified in positive and negative ion modes, including 46 upregulated and 47 downregulated compounds in the Se-deprived group. The differential metabolites were annotated as the major molecules in bile acid biosynthesis, biosynthesis of unsaturated fatty acids, and pyrimidine metabolism, respectively. This study systematically analyzed the serum metabolomics characteristics of P. przewalskii under Se-deprived conditions for the first time, providing a basis for further understanding of the metabolic mechanism of P. przewalskii in the Se-deprived environment.

Boiling has important effects on the texture of chicken eggs, but its effects on egg nutrients have not been systematically investigated. In this study, changes in the metabolites of egg yolks boiled under different heating intensities were quantified and compared by metabolomic analysis. A total of 797 metabolites were identified, and the abundance of 162 metabolites changed significantly after boiling. The significant reduction of L-lysine and D-fructose suggested that Maillard reactions occurred in over-boiled egg yolks. Egg yolk endogenous enzymes might induce a partial hydrolysis of proteins and phospholipids during the warm-up period of boiling, as the abundance of dipeptides, lysophospholipids, and free fatty acids was significantly increased in boiled egg yolks. Boiling increased the detectable abundance of fat-soluble vitamins, riboflavin, and biotin, possibly by altering the complex structure of protein-lipid-lipophilic compounds or denaturing vitamin-binding proteins. The results of metabolomic analyses provide important information for understanding the nutritional changes of egg yolk boiled under different heating intensities.

The Procapra przewalskii inhabits in a selenium (Se)-deprived environment in long-term, but they have no pathological manifestations due to the Se deprivation. This study aimed to reveal the underlying adaptation induced by Se deprivation. In the analysis, a total of 93 significantly changed metabolites were identified in positive and negative ion modes, including 46 upregulated and 47 downregulated compounds in the Se-deprived group. The differential metabolites were annotated as the major molecules in bile acid biosynthesis, biosynthesis of unsaturated fatty acids, and pyrimidine metabolism, respectively. This study systematically analyzed the serum metabolomics characteristics of P. przewalskii under Se-deprived conditions for the first time, providing a basis for further understanding of the metabolic mechanism of P. przewalskii in the Se-deprived environment.

To investigate the effects of nano-ammonium octamolybdate (nano-Mo) and Macleaya cordata extracts (MCE) on antioxidant capacity of grazing Nanjiang brown goats on natural prairies under Cu and Cd stress, a 2 × 2 factorial design was adopted in this test, and two factors were nano-Mo (0, 10 mg/kg) and MCE (0, 3000 mg/kg). 24 hm2 polluted grassland was used in this 30-day trial and was equally divided into twelve fenced units. A total of 36 Nanjiang brown goats (1 year old) with an average body weight (BW) of 40.9 ± 2.1 kg were used in this test. The dietary treatments were (1) CON group, basic diet; (2) Nano-Mo group, basic diet + 10 mg/kg added Mo (nano-Mo); (3) MCE group, basic diet + 3000 mg/kg added MCE; and (4) combined group, basic diet + 10 mg/kg added nano-Mo and 3000 mg/kg added MCE. Nano-Mo or combination of nano-Mo and MCE diets significantly decreased the Cu content in serum and the liver of grazing goats (P