1. [Untitled]
- Author
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Siridechadilok, B, Gomutsukhavadee, M, Sawaengpol, T, Sangiambut, S, Puttikhunt, C, Chin-inmanu, K, Suriyaphol, P, Malasit, P, Screaton, G, and Mongkolsapaya, J
- Subjects
viruses ,Immunology ,Mutant ,Computational biology ,Biology ,Microbiology ,Virus ,03 medical and health sciences ,Viral genetics ,Virology ,RNA Viruses ,Genomic library ,Throughput (business) ,Gene Library ,030304 developmental biology ,0303 health sciences ,030306 microbiology ,RNA ,RNA virus ,biology.organism_classification ,Phenotype ,Genome Replication and Regulation of Viral Gene Expression ,Insect Science ,RNA, Viral ,Genetic Engineering - Abstract
Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses.