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9 results on '"Pulz, M."'

1. How to deal with multidrug-resistant Gram-negative microorganisms in cross-border healthcare? Comparison of Dutch and German guidelines

Author

Müller, J., Voss, A., Köck, R., Sinha, B., Rossen, J.W., Kaase, M., Mielke, M., Daniels-Haardt, I., Jurke, A., Hendrix, R., Kluytmans, J.A., Klytmans-van den Bergh, M.F., Pulz, M., Herrmann, J., and Friedrich, A.W.

Subjects
lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4], GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries)
Abstract

Contains fulltext : 153014.pdf (Publisher’s version ) (Open Access)

Published
2015

7. Prioritization of communicable infectious agents in terms of surveillance and epidemiological research: Results of a standardized procedure in Germany in 2011,Priorisierung übertragbarer infektionserreger unter dem aspekt der surveillance und epidemiologischen forschung: Ergebnisse eines standardisierten vorgehens in Deutschland 2011

Author

Buda, S., Burger, R., Eckmanns, T., Andreas Gilsdorf, Haas, W., Hamouda, O., Lenz, Y., Mielke, M., Stark, K., Krause, G., Wichmann, O., Gärtner, B., Groß, U., Hübner, J., Jänisch, T., Kist, M., Kramer, M. H., Ledig, T., Pulz, M., Suttorp, N., and Ulbrich, U.

9. Decline of Fibroblast Chemotaxis with Age of Donor and Cell Passage Number

Author

B. F. Pontz, Allavena G, Peter Müller, Hartwig Mensing, Matthias Pulz, Adriana Albini, Albini, A, Pontz, B, Pulz, M, Allavena, G, Mensing, H, and Muller, P

Subjects
Male, Premature aging, Aging, Proline, Cell Survival, Fluorescent Antibody Technique, Connective tissue, Biology, Extracellular matrix, Progeria, Rheumatology, medicine, Humans, Fibroblast, Cells, Cultured, Aged, Aged *Aging Cell Division Cell Survival Cells, Cultured *Chemotaxis/drug effects Child, Preschool Collagen/biosynthesis Female Fibroblasts/cytology/drug effects/pathology/*physiology Fibronectins/analysis/pharmacology Fluorescent Antibody Technique Human Infant Laminin/pharmacology Male Progeria/pathology Proline/metabolism Proteins/biosynthesis Thymidine/metabolism, Fibroblast chemotaxis, Chemotaxis, Infant, Fibroblasts, Fibronectins, Cell biology, Fibronectin, medicine.anatomical_structure, Cell culture, Child, Preschool, Protein Biosynthesis, Immunology, biology.protein, Female, Collagen, Laminin, Cell Division, Thymidine
Abstract

Human dermal fibroblasts have a limited life span in culture, which is manifested by aprogressive decline of their proliferative activity. Here we show by the Boyden Chamber assay that the chemotactic response of human fibroblasts to fib rob last-conditioned medium and fibronectin declines during cellular aging in vitro and in vivo. The chemotactic response of human embryonic fibroblasts (HEF) declined progressively after the 25th passage. Virtually no chemotactic activity could be observed after the 40th passage in culture. Fibroblasts cultures from donors aged between 70-90 years had lost chemotactic activity by the 15th passage. Cells from patients suffering from progeroid syndromes of premature aging showed, even in early passages, a very low chemotactic response (20% of the HEF) and lost their chemotactic activity after a few subcultures. The response to the chemoattractant fibronectin also decreased with aging. Immuno-fluorescence studies indicated that the decline in chemotactic activity was accompanied by the formation of a thicker fibronectin network in the extracellular matrix of senescent human fibroblasts and progeroid cells than that observed in early passage embryonic cultures. Since fibroblast chemotaxis and synthesis of connective tissue components probably play an important role in tissue repair, our results could contribute to an understanding of age-related differences in the healing of skin wounds.

Published
1988

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