48 results on '"Piva, F"'
Search Results
2. III-N optoelectronics: defects, reliability and challenges
- Author
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Meneghini, M., De Santi, C., Buffolo, M., Caria, A., Piva, F., Casu, C., Roccato, N., Carlin, J. F., Grandjean, N., Tibaldi, A., Bertazzi, F., Goano, M., Verzellesi, G., Trivellin, N., Meneghesso, G., and Zanoni, E.
- Published
- 2022
3. DLTS-based defect analysis in UV-C single QW LEDs during a constant current stress
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Piva, F., Buffolo, M., De Santi, C., Pilati, M., Roccato, N., Susilo, N., Guttmann, M., Sulmoni, L., Wernicke, T., Kneissl, M., Meneghesso, G., Zanoni, E., and Meneghini, M.
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- 2022
4. Reliability of commercial UV-C LEDs for disinfection purposes
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Trivellin, N., Piva, F., Fiorimonte, D., Buffolo, M., De Santi, C., Zanoni, E., Meneghesso, G., and Meneghini, M.
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- 2022
5. Discriminating the effects of deep-levels in InGaN/GaN LEDs: impact on forward leakage current
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Buffolo, M., Roccato, N., Piva, F., De Santi, C., Haller, C., Jean-François, Carlin, Grandjean, N., Vallone, M., Tibaldic, A., Bertazzi, F., Goano, M., Verzellesi, G., Meneghesso, G., Zanoni, E., and Meneghini, M.
- Published
- 2022
6. The Importance of Saliva for Biomolecule Sampling
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Piva F, Giovanni Principato, A. Righetti, and Matteo Giulietti
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chemistry.chemical_classification ,Saliva ,business.industry ,Biomolecule ,Nanotechnology ,stomatognathic diseases ,fluids and secretions ,stomatognathic system ,chemistry ,Biochemistry ,Biomarker (medicine) ,Medicine ,business ,Blood sampling - Abstract
The oral compartment is an interesting source of biomolecules that could support or even replace blood sampling. Although having the evident advantage of minimal invasiveness, saliva employment for biomarker recovery did not gain ground because it was believed that only few blood molecules could be recovered from saliva. Recent evidence shows that, not only most blood molecules can be found in saliva but also that in saliva there are molecules not present in blood. Moreover saliva contains molecules coming from distant sites such as exosomes. Here we discuss recent evidence that makes saliva promising for biomarker sampling.
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- 2017
7. A possible S-glutathionylation of specific proteins by glyoxalase II: An in vitro and in silico study
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Ercolani, L, Scire, A, Galeazzi, R, Massaccesi, L, Cianfruglia, L, Amici, A, Piva, F, Urbanelli, L, Emiliani, C, Principato, G, and Armeni, T
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MDH protein ,S-d-lactoylglutathione ,actin protein ,glutathione ,glutathionylation ,Glyoxalase II ,glyoxalase system ,post-translational modification (PTM) - Published
- 2016
8. Expression and differential effects of the activation of glucocorticoid receptors in mouse gonadotropin-releasing hormone neurons
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Dondi D, Piccolella M, Messi E, Demissie M, Cariboni A, Selleri S, Piva F, Samara A, Maggi R., CONSALEZ , GIAN GIACOMO, Dondi, D, Piccolella, M, Messi, E, Demissie, M, Cariboni, A, Selleri, S, Piva, F, Samara, A, Consalez, GIAN GIACOMO, and Maggi, R.
- Published
- 2005
9. Factors involved in the migration of neuroendocrine hypothalamic neurons
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Maggi, R., Cariboni, A., Zaninetti, R., Samara, A., Stossi, F., Pimpinelli, F., Giacobini, P., Gian Giacomo Consalez, Rugarli, E., Piva, F., Maggi, R, Cariboni, A, Zaninetti, R, Samara, A, Stossi, F, Pimpinelli, F, Giacobini, P, Consalez, GIAN GIACOMO, Rugarli, E, and Piva, F.
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Gonadotropin-Releasing Hormone ,Neurons ,Hypothalamo-Hypophyseal System ,Cell Movement ,Hypothalamus ,Animals ,Gene Expression Regulation, Developmental ,Humans ,Nerve Growth Factors ,Neurosecretory Systems ,Signal Transduction - Abstract
Neuroendocrine control of physiological functions needs a complex developmental organisation of the hypothalamic parvicellular neurons, which synthesise and release hypophysiotropic hormones. Among the hypothalamic neuroendocrine cells, Gonadotropin-releasing hormone (GnRH) neurons represent a unique class; they are generated in the olfactory placode and, during embryonic life, migrate to the septo/hypothalamic region along terminal and vomeronasal nerves. At this level GnRH neurons undergo terminal differentiation and start to release GnRH to modulate the secretion of pituitary gonadotropins. All these steps are under the strict control of several developmental cues and their defect might represent a cause of clinical disorders. A number of factors have been proposed to be involved in the migration of GnRH neurons, but their role is still unclear. By using gene knockout techniques it has been found that mice carrying a targeted deletion of Ebf2 gene, a component of Olf/Ebf bHLH transcription factors, show a defective migration of GnRH neurons, providing the first evidence of a mouse model of such defect. Since the investigation of GnRH neurons is hindered by their peculiar anatomical distribution, other studies has been forwarded by the availability of immortalized GnRH-expressing neurons (GN11 cells) that retain a strong chemomigratory response "in vitro". Among the factors analysed, we found that hepatocyte growth factor/scatter factor (HGF/SF) and vascular endothelial growth factor (VEGF) induce specific chemotaxis of GN 11 neurons, suggesting that migratory signals can arise from nasal mesenchyme and from the concomitant vasculogenesis. Finally, anosmin-1 (the product of the gene responsible of the X-linked form of Kallmann's disease) was found to induce a significant chemotactic response of GN11 cells, confirming a permissive/instructive role of KAL1 gene product in the migratory behaviour of GnRH neurons. In conclusion, the migration of the GnRH neurons appears to be a complex process, which involves the interplay of multiple molecular cues. These studies may provide new insights on the etiopathogenesis of the large proportion of reproductive dysfunctions that affect humans and could provide novel insights on common biochemical events controlling neuronal development and migration.
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- 2005
10. Biological effects of prolonged exposure to ELF electromagnetic fields in rats: III. 50 Hz electromagnetic fields
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Zecca, L., Mantegazza, C., Margonato, V., Cerretelli, P., Mario Caniatti, Piva, F., Dondi, D., and Hagino, N.
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Physiology ,Biophysics ,Radiology, Nuclear Medicine and imaging ,General Medicine - Published
- 1998
11. Storia di Leda. Da bracciante a dirigente di partito
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Piva, F
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sindacato ,partito comunista italiano ,lotte contadine ,formazione ,Settore M-STO/04 - Storia Contemporanea ,storia di genere - Published
- 2009
12. A possible regulatory role of 17beta-estradiol and tamoxifen on glyoxalase I and glyoxalase II genes expression in MCF7 and BT20 human breast cancer cells
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Rulli, Antonio, Antognelli, Cinzia, Pressi, Eleonora, Baldraccini, F., Piva, F., Giovannini, Elvio, and Talesa, Vincenzo Nicola
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- 2006
13. Educare alla 'purezza': i dilemmi della Gioventù cattolica nel secondo dopoguerra
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Piva, F
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giovani ,formazione ,Chiesa ,Italia 1945-60 ,Settore M-STO/04 - Storia Contemporanea - Published
- 2005
14. L'incontro etnografico in De Martino: riflessioni introduttive
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Piva, F
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Ernesto De Martino ,globalizzazione ,Settore M-STO/04 - Storia Contemporanea ,identità - Published
- 2005
15. Effetti dell'esposizione cronica a CEM di 50 Hz su neurotrasmettitori e recettori nel cervello di ratto
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Zecca L., Costi P., Piva F., Dondi D, and Zucca F.A.
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- 2004
16. 'La Gioventù Cattolica in cammino...' : Memoria e storia del gruppo dirigente (1946-1954)
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Piva, F
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movimento cattolico ,formazione dirigenti ,giovani ,modello educativo ,Settore M-STO/04 - Storia Contemporanea - Published
- 2003
17. Experimental Study of Kinetics of Cellulose Fast Pyrolysis in a Flow Reactor
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Visentin, V., Piva, F., and Canu, Paolo
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- 2002
18. Improved dissolution behaviour of fenbufen by spherical crystallization process
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DI MARTINO, Piera, Barthélémy, C., Piva, F., Joiris, E., Palmieri, Giovanni Filippo, and Martelli, S.
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- 1999
19. Thermal analysis and powder X-ray diffraction study of terpin: evidence of a eutectic 'hydrate/anhydrous form'
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DI MARTINO, Piera, Piva, F, Conflant, P, and GUYOT HERMANN, A. M.
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- 1999
20. Azienda e partito : Gli operai del Poligrafico dello Stato nel periodo fascista
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Piva, F
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operai ,Stato ,Settore M-STO/04 - Storia Contemporanea ,fascismo ,industria tipografica ,disciplina di fabbrica ,operaie - Published
- 1998
21. Il sistema industriale del Friuli-Venezia Giulia, Indirizzi territoriali in materia di localizzazione industriale
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Fabbro, Sandro, Cecotti, M, and Piva, F.
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- 1995
22. Contadini in fabbrica : Il caso Marghera: 1920-1945
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Piva, F
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operai ,contadini ,identità sociale ,Settore M-STO/04 - Storia Contemporanea ,fascismo ,provincia Venezia ,disciplina di fabbrica - Published
- 1991
23. Peptide-steroid interactions in the modulation of the hypothalamic-pituitary axis
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Martini, L., Dondi, D., Patrizia Limonta, Roberto Maggi, Motta, M., and Piva, F.
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Male ,Aging ,Hypothalamo-Hypophyseal System ,Morphine ,Naloxone ,Receptors, Opioid, kappa ,Receptors, Opioid, mu ,Brain ,Luteinizing Hormone ,Cerebral Ventricles ,Prolactin ,Rats ,Estrus ,Receptors, Opioid ,Animals ,Female ,Follicle Stimulating Hormone ,Orchiectomy - Published
- 1987
24. Lotte contadine e origini del fascismo : Padova-Venezia: 1919-1922
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Piva, F
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Veneto ,movimento contadino ,leghe rosse ,leghe bianche ,origini fascismo ,Settore M-STO/04 - Storia Contemporanea - Published
- 1977
25. Effects of progesterone on the central nervous system
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Motta, M., Piva, F., and Martini, L.
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- 1969
- Full Text
- View/download PDF
26. Correlation finder
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Piva, F. and Giovanni PRINCIPATO
27. Impact of VEGF, VEGFR, PDGFR, HIF and ERCC1 gene polymorphisms on thymic malignancies outcome after thymectomy
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Berardi R, Alessandro Brunelli, Pagliaretta S, Paolucci V, Conti A, Goteri G, Refai M, Pompili C, Marcantognini G, Morgese F, Ballatore Z, Savini A, De Lisa M, Caramanti M, Santoni M, Zizzi A, Piva F, Mazzanti P, Onofri A, and Sabbatini A
28. Ageing of the neuroendocrine system in the brain of male rats: receptor mechanisms and steroid metabolism
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Piva, F., Fabio Celotti, Dondi, D., Patrizia Limonta, Roberto Maggi, Elio Messi, Paola Negri-Cesi, Zanisi, M., Motta, M., and Martini, L.
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Male ,Aging ,Hypothalamo-Hypophyseal System ,Brain ,Dihydrotestosterone ,Androstane-3,17-diol ,Neurosecretory Systems ,Rats ,Gonadotropin-Releasing Hormone ,Receptors, Opioid ,Testis ,Animals ,Testosterone ,Receptors, LHRH - Abstract
The work described in this article gives information on the effects of ageing on the hypothalamo-pituitary-testicular axis in rats. The hypothalami of young and old male rats contain similar amounts of luteinizing-hormone-releasing hormone (LHRH); when perifused in vitro they release comparable amounts of LHRH under basal conditions and in response to K+. The addition of an LHRH analogue to the perifusion medium blocks the release of LHRH induced by K+ from the hypothalami of young and old male rats, indicating that the ultrashort feedback mechanism controlling LHRH release functions normally in aged male rats. Ageing also exerts important effects on the density of mu- and kappa-opioid receptors in the brain. The number of hypothalamic mu-opioid receptors was significantly decreased in aged animals; a replacement treatment with testosterone does not reverse this decrease, indicating that the decline of hypothalamic mu receptors and of serum titres of testosterone in old rats are independent phenomena. The number of kappa-opioid receptors in the brain increases in the amygdala and in the thalamus with ageing. Apparently ageing does not influence the number of delta receptors in any of the brain areas investigated. The number of pituitary LHRH receptors decreases in old animals, which might explain the low serum concentration of gonadotrophins in aged rats caused by an inadequate response of the pituitary to hypothalamic LHRH. The impaired secretion of testosterone in aged male rats is accompanied by an increase in the number of testicular LHRH receptors, indicating that the intratesticular mechanisms controlling testosterone release also undergo significant alterations during ageing. The rate of conversion of testosterone to dihydrotestosterone (DHT) and 5 alpha-androstane-3 alpha, 17 beta-diol (3 alpha-diol) is the same in the hypothalami of young and old rats. However, the yields of DHT obtained from the pituitaries of aged male rats are significantly lower than those recorded in the pituitaries of young animals. These results show that the enzymes necessary for metabolizing testosterone via the 5 alpha-reductase pathway are maintained both in the hypothalamus and in the anterior pituitary of aged male rats. However, the 5 alpha-reductase activity of the anterior pituitary of senescent animals appears to be lower than that in the younger controls.
29. Effects of epidermal growth factor on the [3H]-thymidine uptake in the SK-N-SH and SH-SY5Y human neuroblastoma cell lines
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Da Motta, L. A., Galli, P., Piva, F., and Roberto Maggi
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neuroblastoma ,epidermal growth factor ,cultura de células ,growth factors ,fator de crescimento epidérmico ,fatores de crescimento ,cells cultured - Abstract
The studies on the factors that regulate the biology of the neuroblastoma cell lines may offer important information on the development of tissues and organs that derive from the neural crest. In the present paper we study the action of epidermal growth factor (EGF) on two human neuroblastoma cell lines: SK-N-SH which is composed at least of two cellular phenotypes (neuroblastic and melanocytic/glial cells), and its pure neuroblastic subclone SH-SY5Y. The results show that EGF (10 ng/ml) significantly stimulates the incorporation of [3H]-thymidine in the SK-N-SH cells only in the presence of fetal bovine serum (FBS) (control = 58285 ± 9327 cpm; EGF =75523 ± 4457; p
30. How does an In-containing underlayer prevent the propagation of defects in InGaN QW LEDs?: identification of SRH centers and modeling of trap profile
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null Zanoni, null Buffolo, null Piva, null Carlin, Camille Haller, Nicolas Grandjean, null De Santi, null Meneghini, Mauro Mosca, null Meneghesso, null Caria, Fujioka, H, Morkoç, H, Schwarz, UT, Piva, F, De Santi, C, Caria, A, Buffolo, M, Haller, C, Carlin, J-F, Mosca, M, Meneghesso, G, Zanoni, E, Grandjean, N, Meneghini, M, Piva F., De Santi C., Caria A., Buffolo M., Haller C., Carlin J.-F., Mosca M., Meneghesso G., Zanoni E., Grandjean N., and Meneghini M.
- Subjects
Materials science ,LEDs ,business.industry ,underlayer ,growth of defects ,SSPC measurements ,Limiting ,defects concentration ,Settore ING-INF/01 - Elettronica ,Capacitance ,law.invention ,Trap (computing) ,Experimental proof ,law ,defects concentration, growth of defects, LEDs, SSPC measurements, underlayer ,Optoelectronics ,business ,Quantum well ,Recombination ,Light-emitting diode - Abstract
Recent reports indicated that the use of an InAlN underlayer (UL) can significantly improve the efficiency of InGaN/GaN quantum well (QW) LEDs. Currently, this result is explained by considering that the UL reduces the density of nonradiative recombination centers in the QWs. However, an experimental proof of the reduction of defects in the QWs is not straightforward. In this paper, we use combined electrical (I-V), optical (L-I), capacitance (C-V), steady-state photocapacitance (SSPC) and light-assisted capacitance-voltage (LCV) measurements to explain why devices with UL have a much higher efficiency than identical LEDs without UL. Specifically, we demonstrated an improvement in both electrical and optical characteristic for the sample with the UL; SSPC measurements revealed a higher concentration of defects in the active region for the sample without the UL (9.2x1015 cm‑3), compared to the sample with UL (0.8x1015 cm-3). In addition, we demonstrated that dominant defects are located near the midgap (EC ≈ 1.8 eV), thus acting as non-radiative recombination centers. Finally, we proposed a model to find the traps distribution in the active region. By comparing model and experimental results, we demonstrate the effectiveness of the UL in blocking the growth of defects in the bulk of the device, preventing their propagation towards the QW. The results presented in this paper give a proof of the effectiveness of the UL in limiting the propagation of defects towards the QWs and an experimental characterization of the related traps.
- Published
- 2021
31. Defects in III-N LEDs: experimental identification and impact on electro-optical characteristics
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Matteo Buffolo, Nicola Roccato, Francesco Piva, Carlo De Santi, Riccardo Brescancin, Claudia Casu, Alessandro Caria, Kalparupa Mukherjee, Camille Haller, Jean Francois Carlin, Nicolas Grandjean, Marco Vallone, Alberto Tibaldi, Francesco Bertazzi, Michele Goano, Giovanni Verzellesi, Mauro Mosca, Gaudenzio Meneghesso, Enrico Zanoni, Matteo Meneghini, Strassburg, Martin, Buffolo M., Roccato N., Piva F., De Santi C., Brescancin R., Casu C., Caria A., Mukherjee K., Haller C., Carlin J.F., Grandjean N., Vallone M., Tibaldi A., Bertazzi F., Goano M., Verzellesi G., Mosca M., Meneghesso G., Zanoni E., and Meneghini M.
- Subjects
reliability ,gallium nitride ,defects ,indium ,trap-assisted tunneling ,gallium nitride, defects, indium, reliability, trap-assisted tunneling ,Settore ING-INF/01 - Elettronica - Abstract
III-N light-emitting-diodes (LEDs) are subject of intense investigations, thanks to their high efficiency and great reliability. The quality of the semiconductor material has a significant impact on the electro-optical performance of LEDs: for this reason, a detailed characterization of defect properties and the modeling of the impact of defects on device performance are of fundamental importance. This presentation addresses this issue, by discussing a set of recent case studies on the topic; specifically, we focus on the experimental characterization of defects, and on the modeling of their impact on the electro-optical characteristics of the devices.
- Published
- 2022
32. Defect incorporation in In-containing layers and quantum wells: Experimental analysis via deep level profiling and optical spectroscopy
- Author
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Alessandro Caria, J.-F. Carlin, Nicolas Grandjean, C. De Santi, Mauro Mosca, Enrico Zanoni, Matteo Meneghini, F. Piva, Camille Haller, Gaudenzio Meneghesso, Piva F., De Santi C., Caria A., Haller C., Carlin J.F., Mosca M., Meneghesso G., Zanoni E., Grandjean N., and Meneghini M.
- Subjects
Profiling (computer programming) ,Materials science ,Acoustics and Ultrasonics ,Deep level ,InGaN ,business.industry ,underlayer ,SSPC measurements ,Condensed Matter Physics ,Settore ING-INF/01 - Elettronica ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,defects growth ,efficiency ,Optoelectronics ,Spectroscopy ,business ,performance ,Quantum well - Abstract
Recent studies demonstrated that the performance of InGaN/GaN quantum well (QW) light emitting diodes (LEDs) can be significantly improved through the insertion of an InGaN underlayer (UL). The current working hypothesis is that the presence of the UL reduces the density of non-radiative recombination centers (NRCs) in the QW itself: during the growth of the UL, surface defects are effectively buried in the UL, without propagating towards the QW region. Despite the importance of this hypothesis, the concentration profile of defects in the quantum wells of LEDs with and without the UL was never investigated in detail. This paper uses combined capacitance-voltage and steady-state photocapacitance measurements to experimentally identify the defects acting as NRCs and to extract a depth-profile of the traps, thus proving the incorporation upon indium-reaction. Specifically: (i) we demonstrate that LEDs without UL have a high density (9.2 × 1015 cm−3) of defects, compared to samples with UL (0.8 × 1015 cm−3); (ii) defects are located near midgap (E C-1.8 eV, corresponding to E i-E T ∼ 0.3 eV), thus acting as efficient NRCs; (iii) crucially, the density of defects has a peak within the QWs, indicating that traps are segregated at the first grown InGaN layers; (iv) we propose a model to calculate trap distribution in the QW, and we demonstrate a good correspondence with experimental data. These results provide unambiguous demonstration of the role of UL in limiting the propagation of defects towards the QWs, and the first experimental characterization of the properties of the related traps.
- Published
- 2021
33. Real-World Data on Cabozantinib in Previously Treated Patients with Metastatic Renal Cell Carcinoma: Focus on Sequences and Prognostic Factors
- Author
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Alessia Cimadamore, Camillo Porta, Giacomo Cartenì, Paolo Andrea Zucali, Cinzia Ortega, Roberto Iacovelli, Matteo Santoni, Daniele Santini, Alessandra Mosca, Erin Pierce, Marc R. Matrana, Elena Verzoni, Orazio Caffo, Michele Milella, Rodolfo Montironi, Sebastiano Buti, Jeffrey Graham, Sara Merler, Francesco Carrozza, Sergio Bracarda, Marina Scarpelli, Umberto Basso, Francesco Massari, Francesco Piva, Liang Cheng, Vittorio Paolucci, Angelo Martignetti, Franco Morelli, Cristina Masini, Fabio Calabrò, Giuseppe Fornarini, Sarah Scagliarini, Lorena Incorvaia, Nuno Vau, Mimma Rizzo, Francesco Atzori, Alain Gelibter, Riccardo Ricotta, Antonio Lopez-Beltran, Maria Giuseppa Vitale, Ugo De Giorgi, Simon J. Crabb, Giulia Sorgentoni, Pierangela Sepe, Luca Galli, Giuseppe Procopio, Daniel Y. Heng, Alessandro Conti, Nicola Battelli, Santoni M., Heng D.Y., Bracarda S., Procopio G., Milella M., Porta C., Matrana M.R., Carteni G., Crabb S.J., De Giorgi U., Basso U., Masini C., Calabro F., Vitale M.G., Santini D., Massari F., Galli L., Fornarini G., Ricotta R., Buti S., Zucali P., Caffo O., Morelli F., Carrozza F., Martignetti A., Gelibter A., Iacovelli R., Mosca A., Atzori F., Vau N., Incorvaia L., Ortega C., Scarpelli M., Lopez-Beltran A., Cheng L., Paolucci V., Graham J., Pierce E., Scagliarini S., Sepe P., Verzoni E., Merler S., Rizzo M., Sorgentoni G., Conti A., Piva F., Cimadamore A., Montironi R., and Battelli N.
- Subjects
0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,renal cell carcinoma ,Cabozantinib ,Prognosi ,Context (language use) ,urologic and male genital diseases ,lcsh:RC254-282 ,Article ,Pazopanib ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Renal cell carcinoma ,cabozantinib ,Internal medicine ,medicine ,Progression-free survival ,Nivolumab ,Prognosis ,Real-world data ,Targeted therapy ,nivolumab ,real-world data ,business.industry ,Sunitinib ,Retrospective cohort study ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,targeted therapy ,Axitinib ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,prognosis ,business ,medicine.drug - Abstract
Cabozantinib is approved for the treatment of renal cell carcinoma (RCC). However, prognostic factors are still lacking in this context. The aim of this study was to evaluate prognostic factors in RCC patients treated with second- or third-line cabozantinib. A multicenter retrospective real-world study was conducted, involving 32 worldwide centers. A total of 237 patients with histologically confirmed clear-cell and non-clear-cell RCC who received cabozantinib as second- or third-line therapy for metastatic disease were included. We analyzed overall survival (OS), progression-free survival (PFS) and time-to-strategy failure (TTSF) using Kaplan&ndash, Meier curves. Cox proportional models were used at univariate and multivariate analyses.The median PFS and OS of cabozantinib were 7.76 months (95% CI 6.51&ndash, 10.88) and 11.57 months (95% CI 10.90&ndash, not reached (NR)) as second-line and 11.38 months (95% CI 5.79&ndash, NR) and NR (95% CI 11.51&ndash, NR) as third-line therapy. The median TTSF and OS were 11.57 and 15.52 months with the sequence of cabozantinib&ndash, nivolumab and 25.64 months and NR with nivolumab&ndash, cabozantinib, respectively. The difference between these two sequences was statistically significant only in good-risk patients. In the second-line setting, hemoglobin (Hb) levels (HR= 2.39, 95% CI 1.24&ndash, 4.60, p = 0.009) and IMDC (International Metastatic Renal Cell Carcinoma Database Consortium) group (HR = 1.72, 95% CI 1.04&ndash, 2.87, p = 0.037) were associated with PFS while ECOG-PS (HR = 2.33, 95%CI, 1.16&ndash, 4.69, p = 0.018) and Hb levels (HR = 3.12, 95%CI 1.18&ndash, 8.26, p = 0.023) correlated with OS at multivariate analysis, while in the third-line setting, only Hb levels (HR = 2.72, 95%CI 1.04&ndash, 7.09, p = 0.042) were associated with OS. Results are limited by the retrospective nature of the study.This real-world study provides evidence on the presence of prognostic factors in RCC patients receiving cabozantinib.
- Published
- 2019
- Full Text
- View/download PDF
34. Clinical impact of different exosomes’ protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy
- Author
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Giulia Ricci, Alessandra Righetti, Alberto Murrone, Francesca Bianchi, Francesco Piva, Consuelo Amantini, Riccardo Giampieri, Giulia Occhipinti, Silvia Pagliaretta, Alessandro Bittoni, Rossana Berardi, Stefano Cascinu, Giovanni Principato, Matteo Giulietti, Giorgio Santoni, Giampieri, R., Piva, F., Occhipinti, G., Bittoni, A., Righetti, A., Pagliaretta, S., Murrone, A., Bianchi, F., Amantini, C., Giulietti, M., Ricci, G., Principato, G., Santoni, G., Berardi, R., and Cascinu, S.
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0301 basic medicine ,Oncology ,Male ,Integrins ,Colorectal cancer ,medicine.medical_treatment ,Cancer Treatment ,Gene Expression ,Kaplan-Meier Estimate ,Exosomes ,Biochemistry ,Metastasis ,0302 clinical medicine ,Medicine and Health Sciences ,Medicine ,Enzyme-Linked Immunoassays ,Multidisciplinary ,Pharmaceutics ,Liver Diseases ,Middle Aged ,Prognosis ,Epithelial Cell Adhesion Molecule ,Extracellular Matrix ,Gene Expression Regulation, Neoplastic ,030220 oncology & carcinogenesis ,Disease Progression ,Female ,Sample collection ,Cellular Structures and Organelles ,Research Article ,Carcinoma, Pancreatic Ductal ,Clinical Oncology ,Adult ,medicine.medical_specialty ,Science ,Gastroenterology and Hepatology ,Adenocarcinoma ,Research and Analysis Methods ,Disease-Free Survival ,03 medical and health sciences ,Cancer Chemotherapy ,Drug Therapy ,Diagnostic Medicine ,Internal medicine ,Pancreatic cancer ,Carcinoma ,Cell Adhesion ,Biomarkers, Tumor ,Chemotherapy ,Humans ,Progression-free survival ,Vesicles ,Immunoassays ,Aged ,business.industry ,Cancer ,Biology and Life Sciences ,Cell Biology ,medicine.disease ,Pancreatic Neoplasms ,030104 developmental biology ,Immunologic Techniques ,Clinical Medicine ,business ,Transcriptome ,Biomarkers - Abstract
IntroductionPancreatic ductal adenocarcinoma is associated to dismal prognosis despite the use of palliative chemotherapy, partly due to the lack of knowledge of biological processes underlying disease progression. Exosomes have been identified as biomarkers sources in different cancer types. Aim of the study was to analyse the contents of circulating exosomes in patients with pancreatic cancer who received palliative chemotherapy.Patients and methodsPatients were submitted to blood sample collection before chemotherapy (T0) and after 3 months (T3). We quantified by an ELISA-based technique specific proteins of cancer-derived exosomes (CD44,CD44v6,EpCAM,CD9,CD81,Tspan8,Integrin α6,Integrin β4,CD24,CXCR4). We correlated the baseline levels of these factors and changes between T3 and T0 and survival outcomes. Survival analyses were performed by Kaplan-Meier method. Correlation was assessed by log-rank test and level of statistical significance was set at 0.05. Multivariate analysis was performed by logistic regression analysis.ResultsNineteen patients were enrolled. EpCAM T0 levels and increased EpCAM levels from T0 to T3 were those mostly associated with differences in survival. Patients having higher EpCAM had median progression free survival (PFS) of 3.18vs7.31 months (HR:2.82,95%CI:1.03-7.73,p = 0.01). Overall survival (OS) was shorter for patients having higher EpCAM (5.83vs16.45 months,HR:6.16,95%CI:1.93-19.58,p = 0.0001) and also response rates (RR) were worse (20%vs87%,p = 0.015). EpCAM increase during treatment was associated with better median PFS (2.88vs7.31 months,HR:0.24,95%CI:0.04-1.22,p = 0.003). OS was also better (8.75vs11.04 months, HR:0.77,95%CI:0.21-2.73,p = 0.66) and RR were 60%vs20% (p = 0.28). Among clinical factors that might determine changes on PFS and OS, only ECOG PS was associated to significantly worse PFS and OS (p = 0.0137andConclusionsPancreatic cancer patients exosomes express EpCAM, whose levels change during treatment. This represents a useful prognostic factor and also suggests that future treatment modalities who target EpCAM should be tested in pancreatic cancer patients selected by exosome EpCAM expression.
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- 2019
35. Key role of obesity in genitourinary tumors with emphasis on urothelial and prostate cancers
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Matteo Santoni, Antonio Lopez-Beltran, Rodolfo Montironi, Francesco Massari, Lidia Gatto, Liang Cheng, Alessia Cimadamore, Vincenzo Di Nunno, Francesco Piva, Angelo Martignetti, Nicola Battelli, Gaetano Aurilio, Marina Scarpelli, Santoni M., Cimadamore A., Massari F., Piva F., Aurilio G., Martignetti A., Scarpelli M., Di Nunno V., Gatto L., Battelli N., Cheng L., Lopez-Beltran A., and Montironi R.
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0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,Adipose tissue ,Disease ,Review ,lcsh:RC254-282 ,03 medical and health sciences ,Prostate cancer ,chemistry.chemical_compound ,BMI ,0302 clinical medicine ,Internal medicine ,medicine ,Urothelial cancer ,Enzalutamide ,Obesity ,business.industry ,Abiraterone acetate ,Cancer ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Response to therapy ,030104 developmental biology ,Docetaxel ,chemistry ,Cabazitaxel ,030220 oncology & carcinogenesis ,business ,Body mass index ,medicine.drug - Abstract
Background: In human populations, a certain amount of data correlate obesity/body mass index (BMI) with urothelial cancer (UC) and prostate cancer (PCa) occurrence, however this is not fully elucidated at all stages of disease. In an attempt to shed light on uncertain areas in such field, in the present review we illustrate the main molecular mechanisms linking obesity and cancer, focusing on the correlation between obesity and tumor risk, disease progression and response to chemo- and immunotherapy in patients with UC and the predictive/prognostic role of obesity in PCa patients treated with the currently available therapeutic approaches. Methods: We did a large-scale literature search on existing scientific websites focusing on keywords “obesity”, “body mass index (BMI)”, “urothelial cancer”, “prostate cancer”, “docetaxel”, “cabazitaxel”, “abiraterone acetate”, “enzalutamide”, and “radium223”. Results: Many adipocytes-induced molecules support tumor proliferation through activation of various cellular pathways. The available evidence in the postoperative setting do the role of BMI in oncological outcomes prediction still not completely clear. Likewise, in metastatic UC patients controversial results link the role of obesity/BMI with clinical outcomes of tumor response to chemotherapy. Adipose stromal cells recruitment, induced by PCa cells, from white adipose tissue to the tumor sites inducing cell invasiveness was associated with poor survival. Conflicting data, although more oriented towards a better survival outcome, resulted in obese patients treated with docetaxel. In PCa cell-lines a certain cabazitaxel chemo resistance adipose stromal cells (ASC)-mediated was demonstrated. In metastatic castration-resistant PCa patients with high BMI (>25 kg/m2) receiving abiraterone acetate there were significant worse survival outcomes, while in enzalutamide patients BMI did not affect survival outcome. In radium 223 patients higher BMI significantly correlated with favorable overall survival. Conclusions: The main focus of this review was to understand the interplay between obesity/BMI and UC/PCa. Several pathogenic cellular pathways exploring the issue are discussed, opening the way to challenging tailored treatments on the basis of BMI. Improving the knowledge of molecular connections between obesity and UC and PCa could favor the development of new therapies likely reducing chemo- and immunotherapy drug resistance.
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- 2019
36. Phosphorylated mTOR is associated to androgen receptor expression in early triple-negative breast cancer
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F. Piva, Nicola Battelli, M. De Lisa, L. Bastianelli, Mirco Pistelli, Giulia Occhipinti, Stefano Cascinu, Alfredo Santinelli, Zelmira Ballatore, Tommasina Biscotti, R. Bracci, A. Della Mora, Elena Maccaroni, A. Pagliacci, Rossana Berardi, Pistelli, M., Ballatore, Z., Santinelli, A., Biscotti, T., Piva, F., Occhipinti, G., Della Mora, A., Pagliacci, A., Battelli, N., Bastianelli, L., De Lisa, M., Bracci, R., Maccaroni, E., Berardi, R., and Cascinu, S.
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Adult ,0301 basic medicine ,Cancer Research ,Pathology ,medicine.medical_specialty ,Triple Negative Breast Neoplasms ,Biology ,03 medical and health sciences ,Breast cancer ,0302 clinical medicine ,Androgen Receptor Antagonists ,medicine ,Humans ,Phosphorylation ,PI3K/AKT/mTOR pathway ,Triple-negative breast cancer ,Aged ,Cell Proliferation ,Retrospective Studies ,Aged, 80 and over ,Oncogene ,MTOR ,TOR Serine-Threonine Kinases ,Cancer ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Molecular medicine ,Androgen receptor ,030104 developmental biology ,Oncology ,Receptors, Androgen ,Tumor progression ,030220 oncology & carcinogenesis ,Triple-negative ,Disease Progression ,Cancer research ,Female - Abstract
The significance of phosphorylated mTOR (p-mTOR) expression is unknown in triple-negative breast carcinoma (TNBC). The aims of the present study were to assess the expression of p-mTOR in early TNBC and to evaluate possible correlations between androgen receptor (AR) expression, clinicopathological parameters and disease outcome. Between January 2009 and December 2013, all consecutive patients who were diagnosed and completed the treatment of invasive TNBC at our institution were eligible for this analysis. Patients with stage IV disease were excluded. The evaluation of p-mTOR immunohistochemical staining was semi-quantitatively considering both the percentage of positive tumor cells (range, 0-100%) and staining intensity (range, 0-3+). Ninety-eight TNBC patients were included. Approximately 33% of cases were p-mTOR positive and there was no association between positive immunostaining for p-mTOR and DFS (p=0.74) and OS (p=0.81). p-mTOR positivity was associated with small tumor size (p=0.03) and AR expression (p=0.04). High expression of p-mTOR may drive tumor proliferation in almost one third of TNBC. The biological association between mTOR activation and AR pathway suggests that there may exist a subgroup of TNBC in which the combination of both AR antagonism and mTOR inhibition should have a synergistic effect on cell growth and tumor progression.
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- 2016
37. KRAS mutation status is associated with specific pattern of genes expression in pancreatic adenocarcinoma
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Matteo Santoni, Giovanni Principato, Marina Scarpelli, Alessandro Conti, Alessandra Mandolesi, Francesco Piva, Cristian Loretelli, Alessandro Bittoni, Andrea Lanese, Kalliopi Andrikou, Stefano Cascinu, Chiara Pellei, Bittoni, A., Piva, F., Santoni, M., Andrikou, K., Conti, A., Loretelli, C., Mandolesi, A., Lanese, A., Pellei, C., Scarpelli, M., Principato, G., and Cascinu, S.
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Adult ,Male ,Cancer Research ,endocrine system diseases ,overall survival ,Mutant ,pancreatic ductal adenocarcinoma ,Adenocarcinoma ,medicine.disease_cause ,Proto-Oncogene Proteins p21(ras) ,splicing ,Gene expression ,KRAS ,Humans ,Medicine ,neoplasms ,Gene ,Aged ,Aged, 80 and over ,Mutation ,business.industry ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,digestive system diseases ,Neoplasm Proteins ,respiratory tract diseases ,Gene Expression Regulation, Neoplastic ,Pancreatic Neoplasms ,Vascular endothelial growth factor B ,Oncology ,Cancer research ,nucleotide variations ,Female ,business - Abstract
ABSTRACT Aims: To evaluate potential differences at a molecular level between KRAS mutant tumors (MT) and KRAS wild-type (WT) pancreatic tumors and the biological and prognostic significance of different KRAS mutations. Materials & methods: Expression of a panel of 29 genes was analyzed in KRAS WT and MT tumors. Effects of KRAS mutation and gene expression levels were assessed on patients’ survival. Results: MUC6 (p = 0.009), HGF (p = 0.011), VEGFR-2 (p = 0.020) and VEGFB (p = 0.026) were significantly more expressed and SMAD4 was less suppressed (p = 0.003) in WT KRAS. Contrariwise, SHH (p = 0.012) and IHH (p = 0.031) were more expressed in MT KRAS patients. No OS difference was found between WT and MT KRAS tumors. Conclusion: KRAS mutation status seems to identify two different subtypes of pancreatic ductal adenocarcinoma with similar outcome but distinct molecular features and probably different therapeutic targets.
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- 2015
38. Fori imperiali, una proposta. La lezione urbana delle rovine
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F. S. Fera, A. Esposito, PITTINI, SANDRO, BARATA FERNANDES, FRANCISCO JOSÉ, DA ROCHA GONÇALVES DIAS, ADALBERTO, TISSELLI, CARLA, PIVA, FILIPPO, Luca Basso Peressut, Pier Federico Caliari, Fera, F. S., Esposito, A., Pittini, S., Barata Fernandes, F. J., Da Rocha Goncalves Dias, A., Tisselli, C., and Piva, F.
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Fori Imperiali, progetto urbano, museografia - Abstract
Il progetto pubblicato è il risultato della partecipazione alla call internazionale indetta dall'Accademia adrianea di Roma sulla Via dei Fori Imperiali di Roma. Il gruppo del Dipartimento di Architettura dell'Università di Bologna ha formulato la propria proposta in collaborazione con gli studi degli architetti portoghesi Francisco Barata, Adalberto Dias e il paesaggista Filippo Piva. I Fori Imperiali sono stati pensati a partire dalla riproposizione della attuale via dei Fori Imperiali come elemento di giunzione e non di separazione tra i reperti di epoca Repubblicana e quella Imperiale. Un nuovo elemento di grande permeabilità avrebbe dovuto interpretare l'evento di collegamento tra le parti oggi drammaticamente separate tra di loro. Il progetto si è avvalso della collaborazione di esperti internazionali per gli aspetti della composizione e del paesaggio. The project published is the result of the participation in the international call announced by the Hadrian Academy of Rome on the Via dei Fori Imperiali in Rome. The group of the Department of Architecture of the University of Bologna has formulated its proposal in collaboration with the Portuguese architects Francisco Barata, Adalberto Dias and the landscape designer Filippo Piva. The Fori Imperiali were designed from the re-proposal of the current Via dei Fori Imperiali as an element of junction and not of separation between the finds of the Republican period and the Imperial one. A new element of great permeability should have interpreted the event of connection between the parties today dramatically separated from each other. The project availed itself of the collaboration of international experts for the aspects of composition and landscape.
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- 2017
39. HER family receptor expression and prognosis in pancreatic cancer
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Chiara Pellei, Simona Alfonsi, Stefano Cascinu, Marina Scarpelli, Cristian Loretelli, Alessandro Bittoni, Francesco Piva, Andrea Lanese, Kalliopi Andrikou, Alessandra Mandolesi, Matteo Santoni, Bittoni, A., Mandolesi, A., Andrikou, K., Santoni, M., Alfonsi, S., Lanese, A., Loretelli, C., Pellei, C., Piva, F., Scarpelli, M., and Cascinu, S.
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Male ,Cancer Research ,Receptor expression ,EGFR ,Clinical Biochemistry ,Kaplan-Meier Estimate ,Adenocarcinoma ,Pathology and Forensic Medicine ,Breast cancer ,Pancreatectomy ,Pancreatic cancer ,medicine ,Humans ,Single-Blind Method ,Molecular Targeted Therapy ,Receptor ,Aged ,Neoplasm Staging ,Proportional Hazards Models ,Aged, 80 and over ,Lung ,business.industry ,Middle Aged ,medicine.disease ,Prognosis ,Neoplasm Proteins ,ErbB Receptors ,Pancreatic Neoplasms ,medicine.anatomical_structure ,Oncology ,Tumor progression ,HER-2 ,Immunology ,Cancer research ,HER-3 ,Female ,business ,Pancreatic adenocarcinoma ,Dimerization - Abstract
BackgroundHER family receptors play a key role in tumor progression in several malignancies, such as colorectal, lung or breast cancer. The aims of this study were to investigate expression of HER-1, HER-2 and HER-3 in pancreatic cancer (PC) samples and evaluate the association between HER-family receptor expression and patients’ clinical outcomes.MethodsTissue samples from 91 PC patients were subjected to immunohistochemical staining to assess the expression of HER-1, HER-2 and HER-3. Semiquantitative scores of zero (no staining or staining in less than 10% of cancer cells), 1+, 2+ or 3+ were assigned to each sample based on the intensity of staining for HER receptors. Scores of 2+ or 3+ were defined as positive staining.ResultsHER-1 overexpression was observed in 41 out of 91 samples (45.1%), while HER-2 was not overexpressed in any of the analyzed samples. HER-3 was overexpressed in 37 samples (40.7%) and was found to be associated with advanced TNM stage. In particular, HER-3 was overexpressed in 12 out of 16 stage IV patients (75%) compared with only 33.3% of stage I-III patients (p = 0.02). Among 79 patients with available survival data, the 6 patients with strong HER-3 expression (score 3+) had a shorter survival compared with remaining patients (median overall survival 6.9 months vs. 12.3 months, respectively).ConclusionsHER-1 and HER-3 were found to be expressed in a significant proportion of PC patients. Strong HER-3 expression represents an indicator of poor prognosis in PC patients, being associated with advanced stage and shorter survival.
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- 2015
40. Metabolic alterations in renal cell carcinoma
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Rodolfo Montironi, Alessandra Modena, Chiara Ciccarese, Stefano Cascinu, Matteo Santoni, Giampaolo Tortora, Daniele Santini, Liang Cheng, Emanuela Fantinel, Francesco Massari, Francesco Piva, Davide Bimbatti, Matteo Brunelli, Massari, F., Ciccarese, C., Santoni, M., Brunelli, M., Piva, F., Modena, A., Bimbatti, D., Fantinel, E., Santini, D., Cheng, L., Cascinu, S., Montironi, R., and Tortora, G.
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medicine.medical_specialty ,Nutrient sensing ,Biology ,Pentose phosphate pathway ,urologic and male genital diseases ,medicine.disease_cause ,Internal medicine ,medicine ,Animals ,Humans ,Radiology, Nuclear Medicine and imaging ,RCC carcinogenesis ,Carcinoma, Renal Cell ,AMPK ,General Medicine ,Kidney Neoplasms ,female genital diseases and pregnancy complications ,Renal cell carcinoma ,Citric acid cycle ,Metabolic pathway ,Endocrinology ,Oncology ,Anaerobic glycolysis ,Metabolic pathways ,Lipogenesis ,Therapeutic strategies ,Cancer research ,Carcinogenesis ,Metabolic Networks and Pathways - Abstract
Renal cell carcinoma (RCC) is a metabolic disease, being characterized by the dysregulation of metabolic pathways involved in oxygen sensing (VHL/HIF pathway alterations and the subsequent up-regulation of HIF-responsive genes such as VEGF, PDGF, EGF, and glucose transporters GLUT1 and GLUT4, which justify the RCC reliance on aerobic glycolysis), energy sensing (fumarate hydratase-deficient, succinate dehydrogenase-deficient RCC, mutations of HGF/MET pathway resulting in the metabolic Warburg shift marked by RCC increased dependence on aerobic glycolysis and the pentose phosphate shunt, augmented lipogenesis, and reduced AMPK and Krebs cycle activity) and/or nutrient sensing cascade (deregulation of AMPK-TSC1/2-mTOR and PI3K-Akt-mTOR pathways). We analyzed the key metabolic abnormalities underlying RCC carcinogenesis, highlighting those altered pathways that may represent potential targets for the development of more effective therapeutic strategies.
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- 2015
41. Re: Johan Lindberg, Anna Kristiansen, Peter Wiklund, Henrik Gronberg, Lars Egevad. Tracking the Origin of Metastatic Prostate Cancer. Eur Urol 2015; 67: 819-22
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Rodolfo Montironi, Francesco Montorsi, Alberto Briganti, Marina Scarpelli, Francesco Piva, Matteo Santoni, Piva, F, Santoni, M, Scarpelli, M, Briganti, Alberto, Montorsi, Francesco, and Montironi, R.
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Male ,business.industry ,Biopsy ,Urology ,Prostate ,DNA ,medicine.disease ,Carcinoma, Ductal ,Neoplasm Metastasi ,Prostate cancer ,Prostatic Neoplasm ,medicine ,Religious studies ,business ,Human - Published
- 2015
42. PD-1 blockade therapy in renal cell carcinoma. Current studies and future promises
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Rodolfo Montironi, Matteo Santoni, Guido Martignoni, Giampaolo Tortora, Daniele Santini, Matteo Brunelli, Francesco Massari, Chiara Ciccarese, Stefano Cascinu, Camillo Porta, Salvatore Alfieri, F. Piva, Rossana Berardi, Massari, F., Santoni, M., Ciccarese, C., Santini, D., Alfieri, S., Martignoni, G., Brunelli, M., Piva, F., Berardi, R., Montironi, R., Porta, C., Cascinu, Stefano, and Tortora, G.
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Vascular Endothelial Growth Factor A ,T-Lymphocytes ,medicine.medical_treatment ,Programmed Cell Death 1 Receptor ,Cell ,B7-H1 Antigen ,PD-1 ,Immunotherapy ,PDL-1 ,Renal cell carcinoma ,Cytotoxicity ,Clinical Trials as Topic ,renal cell carcinoma ,immunotherapy ,Antibodies, Monoclonal ,General Medicine ,Prognosis ,Kidney Neoplasms ,Gene Expression Regulation, Neoplastic ,Nivolumab ,medicine.anatomical_structure ,Oncology ,Signal Transduction ,medicine.drug ,Down-Regulation ,Antineoplastic Agents ,Ipilimumab ,Antibodies, Monoclonal, Humanized ,Immune system ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Carcinoma, Renal Cell ,Cell Proliferation ,Neoplasm Staging ,business.industry ,Antibody-Dependent Cell Cytotoxicity ,Cancer ,medicine.disease ,Receptors, Vascular Endothelial Growth Factor ,Immunology ,Cancer research ,Neoplasm Grading ,business - Abstract
RCC is considered an immunogenic tumor with a prominent dysfunctional immune cell infiltrate, unable to control tumor growth. Evasion of immune surveillance, a process defined immune-editing, leads to malignant progression. The striking improvement of knowledge in immunology has led to the identification of immune checkpoints (such as CTLA-4 and PD-1), whose blockage enhances the antitumor immunity. The interaction between PD-1, an inducible inhibitory receptor expressed on lymphocytes and DCs, and PD-L1 ligand, expressed by tumor cells, results in a down-regulation of the T-cell response. Therefore, the PD-1/PD-L1 axis inhibition by targeted-antibodies, increasing the T-cell proliferation and cytotoxicity, represents a promising mechanism to stimulate the anti-tumor activity of the immune system, improving the outcomes of cancer patients. Several PD-1 and PD-L1 inhibitors have been evaluated in different tumor types, showing promising results. The interesting correlation between lymphocytes PD-1 expression and RCC advanced stage, grade and prognosis, as well as the selective PD-L1 expression by RCC tumor cells and its potential association with worse clinical outcomes, have led to the development of new anti PD-1/PD-L1 agents, alone or in combination with anti-angiogenic drugs or other immunotherapeutic approaches, for the treatment of RCC. In this review we discuss the role of PD-1/PD-L1 in RCC, focusing on the biological rationale, current clinical studies and promising therapeutic perspectives to target the PD-1 pathway.
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- 2015
43. Presence of delta opioid receptors on a subset of hypothalamic gonadotropin releasing hormone (GnRH) neurons
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Roberto Maggi, Tomas Hökfelt, Marco Parenti, Federica Pimpinelli, Francesca Guzzi, Flavio Piva, Pimpinelli, F, Parenti, M, Guzzi, F, Piva, F, Hokfelt, T, and Maggi, R
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DELTA OPPIOID RECEPTOR ,endocrine system ,medicine.medical_specialty ,Enkephalin ,medicine.drug_class ,Hypothalamus ,Down-Regulation ,Hypothalamus, Middle ,Gonadotropin-releasing hormone ,GTP-Binding Protein alpha Subunits, Gi-Go ,Biology ,Gonadotropin-Releasing Hormone ,Rats, Sprague-Dawley ,GTP-Binding Proteins ,Opioid receptor ,Receptors, Opioid, delta ,Internal medicine ,Cyclic AMP ,medicine ,Animals ,Tissue Distribution ,Opioid peptide ,Receptor ,Molecular Biology ,Cellular Senescence ,Cell Line, Transformed ,Nerve Endings ,Neurons ,General Neuroscience ,Rats ,Endocrinology ,medicine.anatomical_structure ,Median eminence ,Neurology (clinical) ,Neuron ,Enkephalin, D-Penicillamine (2,5) ,hormones, hormone substitutes, and hormone antagonists ,Developmental Biology - Abstract
Opioid peptides exert an inhibitory effect on hypothalamic gonadotropin releasing hormone (GnRH) secretion mainly by interacting with μ-opioid receptors. Although a direct role for opioids via δ-opioid receptors (DORs) has been suggested, the presence of these receptors on GnRH neurons has never been demonstrated. In the present study, we determined the distribution of DORs in the basal hypothalamus of rat with special focus on their relation to GnRH neurons. Double-labelling immunofluorescence and confocal microscopy revealed that DORs are exclusively present in a subpopulation of GnRH nerve terminals, with the highest density in the external layer of the median eminence. We then studied the functional characteristics of DORs in an immortalized GnRH-secreting neuronal cell line (GT1-1) known to endogenously express this receptor. Here, pertussis toxin pretreatment abolished the δ-agonist (DPDPE) inhibitory effect on cAMP accumulation. We also analyzed the type of G proteins involved in the signal transduced by the DOR and showed that GT1-1 cells express the inhibitory Go and Gi2 alpha-subunits. However, only Go was down-regulated under chronic DPDPE exposure. Finally, since DOR is expressed postnatally in brain, we compared GnRH neuronal cells immortalized at different developmental stages (the more mature GT1-1 and GT1-7 cells, versus the more immature GN11 cells), evidencing that only mature neurons express DOR. In conclusion, our study indicates that a direct control of opioids via δ-receptors occurs on GnRH neurons and validates the use of GT1 cells to further investigate the nature of the DOR present on GnRH neurons. © 2005 Elsevier B.V. All rights reserved.
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- 2006
44. Association of GSK-3β genetic variation with GSK-3β expression, prefrontal cortical thickness, prefrontal physiology, and schizophrenia
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Joel E. Kleinman, Alessandro Bertolino, Daniel R. Weinberger, Leonardo Fazio, Antonio Rampino, Rita Masellis, Teresa Popolizio, Giuseppe Blasi, Paolo Taurisano, Lucia Colagiorgio, Matteo Giulietti, Barbara K. Lipska, Francesco Napolitano, Marina Mancini, Annabella Di Giorgio, Maria Teresa Attrotto, Gianluca Ursini, Raffaella Romano, Grazia Caforio, Barbara Gelao, Annamaria Porcelli, Francesco Piva, Giovanna Todarello, Alessandro Usiello, Apostolos Papazacharias, Tiziana Quarto, Blasi, G, Napolitano, F, Ursini, G, Di Giorgio, A, Caforio, G, Taurisano, P, Fazio, L, Gelao, B, Attrotto, Mt, Colagiorgio, L, Todarello, G, Piva, F, Papazacharias, A, Masellis, R, Mancini, M, Porcelli, A, Romano, R, Rampino, A, Quarto, T, Giulietti, M, Lipska, Bk, Kleinman, Je, Popolizio, T, Weinberger, Dr, Usiello, Alessandro, Bertolino, A., Napolitano, Francesco, and Usiello, A
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Adult ,Male ,genotype ,Gene Expression ,Prefrontal Cortex ,macromolecular substances ,Biology ,Polymorphism, Single Nucleotide ,Glycogen Synthase Kinase 3 ,Young Adult ,Neurodevelopmental disorder ,Imaging, Three-Dimensional ,Genotype ,Genetic variation ,Image Interpretation, Computer-Assisted ,medicine ,Humans ,Attention ,Computer Simulation ,Genetic Predisposition to Disease ,RNA, Messenger ,Prefrontal cortex ,GSK3B ,beta Catenin ,Glycogen Synthase Kinase 3 beta ,medicine.disease ,Phenotype ,Magnetic Resonance Imaging ,Psychiatry and Mental health ,Schizophrenia ,Female ,Schizophrenic Psychology ,Signal transduction ,genetic ,Cognition Disorders ,Neuroscience ,Signal Transduction - Abstract
Objective: Glycogen synthase kinase 3 beta (GSK-3 beta) is an enzyme implicated in neurodevelopmental processes with a broad range of substrates mediating several canonical signaling pathways in the brain. The authors investigated the association of variation in the GSK-3 beta gene with a series of progressively more complex phenotypes of relevance to schizophrenia, a neurodevelopmental disorder with strong genetic risk.Method: Based on computer predictions, the authors investigated in humans the association of GSK-3 beta functional variation with 1) GSK-3 beta mRNA expression from postmortem prefrontal cortex, 2) GSK-3 beta and beta-catenin protein expression from peripheral blood mononuclear cells (PBMCs), 3) prefrontal imaging phenotypes, and 4) diagnosis of schizophrenia.Results: Consistent with predictions, the TT genotype of a single-nucleotide polymorphism in GSK-3 beta (rs12630592) was associated with reduced GSK-3 beta mRNA from postmortem prefrontal cortex. Furthermore, this genotype was associated with GSK-3 beta protein expression and kinase activity, as well as with downstream effects on beta-catenin expression in PBMCs. Finally, the TT genotype was associated with attenuated functional MRI prefrontal activity, reduced prefrontal cortical thickness, and diagnosis of schizophrenia.Conclusions: These results suggest that GSK-3 beta variation is implicated in multiple phenotypes relevant to schizophrenia.
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- 2013
45. Cancer stem cell gene profile as predictor of relapse in high risk stage II and stage III, radically resected colon cancer patients
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Michela Del Prete, Francesco Piva, Giovanni Lezoche, Italo Bearzi, Riccardo Giampieri, Mario Scartozzi, Maristella Bianconi, Luca Cecchini, Alessandro Bittoni, Alessandra Mandolesi, Stefano Cascinu, Luca Faloppi, Cristian Loretelli, Mario Guerrieri, Giampieri, R, Scartozzi, M, Loretelli, C, Piva, F, Mandolesi, A, Lezoche, G, Del Prete, M, Bittoni, A, Faloppi, L, Bianconi, M, Cecchini, L, Guerrieri, M, Bearzi, I, and Cascinu, Stefano
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Adult ,Male ,Oncology ,medicine.medical_specialty ,Pathology ,Abcg2 ,Colorectal cancer ,Science ,Disease ,Cancer stem cell ,Internal medicine ,Biomarkers, Tumor ,Humans ,Medicine ,Stage (cooking) ,ALCAM ,Aged ,Neoplasm Staging ,Aged, 80 and over ,Multidisciplinary ,biology ,business.industry ,CD44 ,Middle Aged ,Prognosis ,medicine.disease ,Colonic Neoplasms ,Neoplastic Stem Cells ,biology.protein ,Female ,Neoplasm Recurrence, Local ,Stem cell ,business ,Research Article - Abstract
Clinical data indicate that prognostic stratification of radically resected colorectal cancer based on disease stage only may not be always be adequate. Preclinical findings suggest that cancer stem cells may influence the biological behaviour of colorectal cancer independently from stage: objective of the study was to assess whether a panel of stemness markers were correlated with clinical outcome in resected stage II and III colon cancer patients. A panel of 66 markers of stemness were analysed and thus patients were divided into two groups (A and B) with most patients clustering in a manner consistent with different time to relapse by using a statistical algorithm. A total of 62 patients were analysed. Thirty-six (58%) relapsed during the follow-up period (range 1.63–86.5 months). Twelve (19%) and 50 (81%) patients were allocated into group A and B, respectively. A significantly different median relapse-free survival was observed between the 2 groups (22.18 vs 42.85 months, p = 0.0296). Among of all genes tested, those with the higher “weight” in determining different prognosis were CD44, ALCAM, DTX2, HSPA9, CCNA2, PDX1, MYST1, COL1A1 and ABCG2. This analysis supports the idea that, other than stage, biological variables, such as expression levels of colon cancer stem cell genes, may be relevant in determining an increased risk of relapse in resected colorectal cancer patients.
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- 2013
46. TRAM (Transcriptome Mapper): database-driven creation and analysis of transcriptome maps from multiple sources
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Federica Facchin, Francesco Piva, Alessandro Coppe, Lorenza Vitale, Gian Antonio Danieli, Raffaella Casadei, Maria Chiara Pelleri, Stefania Bortoluzzi, Flavia Frabetti, Luca Lenzi, Silvia Canaider, Matteo Giulietti, Giovanni Principato, Sergio Ferrari, Pierluigi Strippoli, Lenzi L., Facchin F., Piva F., Giulietti M., Pelleri M.C., Frabetti F., Vitale L., Casadei R., Canaider S., Bortoluzzi S., Coppe A., Danieli G.A., Principato G., Ferrari S., and Strippoli P.
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lcsh:QH426-470 ,lcsh:Biotechnology ,Genomics ,Context (language use) ,TRANSCRIPTOME MAP ,Biology ,computer.software_genre ,Models, Biological ,transcriptome map ,bioinformatics ,hematopoietic cells ,Transcriptome ,User-Computer Interface ,lcsh:TP248.13-248.65 ,Gene cluster ,Databases, Genetic ,Genetics ,Cluster Analysis ,Humans ,Quantile normalization ,Internet ,GENE CLUSTER ,GENE EXPRESSION PROFILE ,Gene Expression Profiling ,Computational Biology ,Gene expression profiling ,lcsh:Genetics ,Data format ,Database Management Systems ,Data mining ,DNA microarray ,computer ,GENOMICS ,Biotechnology ,Research Article - Abstract
Background Several tools have been developed to perform global gene expression profile data analysis, to search for specific chromosomal regions whose features meet defined criteria as well as to study neighbouring gene expression. However, most of these tools are tailored for a specific use in a particular context (e.g. they are species-specific, or limited to a particular data format) and they typically accept only gene lists as input. Results TRAM (Transcriptome Mapper) is a new general tool that allows the simple generation and analysis of quantitative transcriptome maps, starting from any source listing gene expression values for a given gene set (e.g. expression microarrays), implemented as a relational database. It includes a parser able to assign univocal and updated gene symbols to gene identifiers from different data sources. Moreover, TRAM is able to perform intra-sample and inter-sample data normalization, including an original variant of quantile normalization (scaled quantile), useful to normalize data from platforms with highly different numbers of investigated genes. When in 'Map' mode, the software generates a quantitative representation of the transcriptome of a sample (or of a pool of samples) and identifies if segments of defined lengths are over/under-expressed compared to the desired threshold. When in 'Cluster' mode, the software searches for a set of over/under-expressed consecutive genes. Statistical significance for all results is calculated with respect to genes localized on the same chromosome or to all genome genes. Transcriptome maps, showing differential expression between two sample groups, relative to two different biological conditions, may be easily generated. We present the results of a biological model test, based on a meta-analysis comparison between a sample pool of human CD34+ hematopoietic progenitor cells and a sample pool of megakaryocytic cells. Biologically relevant chromosomal segments and gene clusters with differential expression during the differentiation toward megakaryocyte were identified. Conclusions TRAM is designed to create, and statistically analyze, quantitative transcriptome maps, based on gene expression data from multiple sources. The release includes FileMaker Pro database management runtime application and it is freely available at http://apollo11.isto.unibo.it/software/, along with preconfigured implementations for mapping of human, mouse and zebrafish transcriptomes.
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- 2011
47. Les Allemands dans la 'trilogie' de Céline
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DISEGNI, SILVIA, De Gasperi Ronc M. L., Pietromarchi L., Piva F., and Disegni, Silvia
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Atti del XVI Convegno di Trento della Società per gli Studi di Lingua e Letteratura Francese: "La letteratura francese e il mondo germanico"
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- 1990
48. Il cantiere navale Breda (1928-1942)
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FELTRIN, PAOLO, Piva F., Tattara G., and Feltrin, Paolo
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Veneto ,mercato del lavoro ,occupazione - Published
- 1983
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