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From a materials perspective, the pillars for the development of clinically translatable scaffold-based strategies for craniomaxillofacial (CMF) bone and periodontal regeneration have included electrospinning and 3D printing (biofabrication) technologies. Here, we offer a detailed analysis of the latest innovations in 3D (bio)printing strategies for CMF bone and periodontal regeneration and provide future directions envisioning the development of advanced 3D architectures for successful clinical translation. First, the principles of electrospinning applied to the generation of biodegradable scaffolds are discussed. Next, we present on extrusion-based 3D printing technologies with a focus on creating scaffolds with improved regenerative capacity. In addition, we offer a critical appraisal on 3D (bio)printing and multitechnology convergence to enable the reconstruction of CMF bones and periodontal tissues. As a future outlook, we highlight future directions associated with the utilisation of complementary biomaterials and (bio)fabrication technologies for effective translation of personalised and functional scaffolds into the clinics.

There is cumulative evidence supporting the negative effects of smoking on periodontal tissues. Smoking cessation can be successfully accomplished through specific programs, including behaviour modification and medications, and has been suggested as a suitable way to reduce the risk of several diseases, including periodontitis. The aim of this review is to provide a concise overview of the current knowledge about the impact of smoking cessation on periodontal tissues and therapy, with data from studies published in the last 15 years. Literature was searched using Medline database from 2005 up to and including September 2020 using medical subject heading (MeSH) terms and other search terms, restricted to the English language. Studies were evaluated and summarised in a narrative review format. Results demonstrated that there is convincing evidence to support the benefits of tobacco cessation in reducing the risk of periodontitis and tooth loss. In addition, the harmful effects of smoking on periodontal tissues seem to be assuaged as the number of years since quitting increases. The existing current evidence, even limited, also shows that smoking cessation may result in additional benefits to the outcome of nonsurgical periodontal treatment. Periodontal care providers should not only check their patient's smoking habit for estimating risk of disease progression and predictability of periodontal therapy, but they should also help smokers improve their oral and systemic health by providing efficient and personalised tobacco-cessation counselling and treatment.

The standard procedure to treat oronasal fistula in dogs requires tooth extraction to close the fistula; hence, the subject would lose its tooth. In this study, trafermin was applied to four dog models with oronasal fistula to investigate the periodontal tissue regenerative effects of trafermin in the treatment without tooth extraction. A fistula was created along the palatal side of each upper canine tooth. One of the fistulae was filled with trafermin, whereas that on the contralateral side was left unfilled as a control. The results showed a significant decrease in the non-calcified periodontal tissue volume on the trafermin side after the fourth week. In addition, oronasal fistula closure was visually and histologically confirmed at the eighth week on the trafermin side of all four models.

The article describes the etiologic and pathogenic relationship of periodontopathogenic microbiota in patients with generalized periodontitis and cardiovascular disease development. The article presents the literature data and the results of own investigation regarding the direct impact of periodontal pathogens on cardiovascular disease etiology due to transitory bacteriemia. The article considers the pathogenetic relationship and the impact of infectious and inflammatory diseases in the oral cavity on the development of cardiovascular disease by stimulating the production of proinflammatory cytokines in periodontal tissues and C-reactive protein by the body.

The article presents modern data on the features of the oral microbiome and its medical significance when prescribing systemic antibacterial therapy for infectious and inflammatory processes in the oral cavity. The main microbial associations in normal conditions, as well as in diseases of periodontal tissues and apical periodontitis are presented. The use of antibacterial drugs in dentistry is aimed at eliminating the etiological factor of the pathogenic microflora of the oral cavity. The effectiveness of the treatment depends on how well the doctor aware of the main pathogens and microbial associations in generalized periodontitis, periodontitis, a choice of optimal antibacterial agent, compliance with indications and contraindications upon treatment., У статті наведені сучасні дані про особливості мікробіому порожнини рота та його медичне значення при призначенні системної антибактеріальної терапії інфекційно-запальних процесів порожнини рота. Наведені основні мікробні асоціації в нормі та при хворобах пародонта й апікальному періодонтиті. Використання антибактеріальних препаратів у стоматології направлене на усунення етіологічного чинника патогенної мікрофлори порожнини рота. Ефективність лікування залежить від інформованості лікаря про основні збудники та мікробні асоціації при генералізованому пародонтиті, періодонтиті, а також від вибору оптимального антибактеріального засобу, дотримання показань і протипоказань при призначенні.

The removal of calculus is a major part of periodontal treatment, in order to improve the clinical situation. Calculus develops on hard tissues in the oral cavity. This can occur both supra- and subgingivally. The rate of its development depends on a variety of factors. Due to the rough surface, calculus is an ideal basis for a (harmful) biofilm. It is therefore a point of debate whether the calculus or the overlaying biofilm can lead to the periodontal inflammation. This in turn raises the question whether only this biofilm needs to be removed or whether the calculus also needs to be removed. As long as ten centuries ago, the removal of calculus was used as a treatment for improving oral health and that is not very different today.In de parodontale behandeling vormt de verwijdering van tandsteen een belangrijk onderdeel om de klinische situatie te verbeteren. Tandsteen vormt zich op harde weefsels in de mondholte. Dit kan zowel supra- als subgingivaal plaatsvinden. De mate waarin dit gebeurt is afhankelijk van veel verschillende factoren. Door het ruwe oppervlak vormt tandsteen een ideale bodem voor een (schadelijke) biofilm. Het is dan ook een punt van discussie of juist het tandsteen of de oppervlakkige biofilm tot een parodontale ontsteking kan leiden. En daar vloeit de vraag uit voort of het dan alleen deze biofilm is die verwijderd moet worden of dat ook het tandsteen verwijderd moet worden. Al meer dan 10 eeuwen geleden werd tandsteen verwijderd voor de verbetering van de mondgezondheid en dat is eigenlijk niet veel anders vandaag de dag.

Introduction. Inflammatory diseases of periodontal tissue are one of the most prevalent worldwide. This pathology has a complex aetiology and ambiguous views on pathogenetic mechanisms; therefore, the approach to the assessment of periodontitis should be integrated. The aim of the study was to assess the state of periodontal tissues in patients with chronic generalized periodontitis class II and absent or compensated somatic pathology. Materials and methods. We examined 20 patients aged 35 to 45 years having a diagnosis of chronic generalized periodontitis class II who had no somatic pathologies or compensated somatic pathology. The patients underwent the assessment of their hygienic, gingival and periodontal indices, measuring the depth of periodontal pockets, the level of the gingival margin, loss of clinical attachment and bleeding during probing. Results. The average value of the Green-Vermillion hygienic index among the study group was 2.16, the papillary-marginal-alveolar index was 56%, and the periodontal Russell index was 3.4scores. The mean value of the depth of periodontal pockets was 3.75, and the loss of clinical attachment was 4.12. The average index of Müllemann's bleeding gums in the surveyed was 4.1. Conclusions. Аn integrated approach to assessing the state of periodontal tissues contributes to a better understanding of the epidemiology, characteristics of the course and predicting the consequences of chronic generalized periodontitis class II.

Relevance of the research topic. The authors found that various risk factors play a leading role in changing the clinical state of periodontal tissues. The aim is a comparative assessment of the clinical condition of periodontal tissues based on the correlation of hygienic and periodontal indices depending on age. Material and methods. A comprehensive clinical examination of 765 young people was conducted. The main group consisted of 70 (66.6%) patients with diagnosed chronic gingivitis and 145 (33.35%) patients with mild initial periodontitis due to the presence of conditionally pathogenic and periodontopathogenic types of microorganisms in the microbial biofilm. The control group was formed by 83 patients whose index evaluation revealed the condition of periodontal tissues in the form of clinically healthy gums with intact periodontal and clinically healthy gums with periodontal tissue loss, respectively, in 61.4 and 38.6% of cases of observations. Study results. In 61.4% of persons forming groups with clinically healthy gums, hygienic and periodontal indices are within normal values, in 38.6% of cases of persons there is a tendency to change them from the norm. In 47.7% and 52.3% of cases of individuals with gingivitis and in 48.3% and 51.7% of individuals with initial periodontitis, the OHI-S index correlates with the indicators of PMA, GI and SBI (p < 0.001, p = 0.315). Conclusions. Thus, the revealed changes in the indicators of hygienic and periodontal indices (the Chuprov conjugacy coefficient is 0.64 (p = 0.012), the Kraskel-Wallis criterion (H = 7.89, p < 0.05) makes it possible to clearly determine the clinical condition of periodontal tissues corresponding to modern European criteria.

Today special attention to the influence of environmental factors on the condition of the dental-maxillary system and periodontal tissues in children is paid. Scientists have proved that environmentally unfavorable conditions also change the immunological reactivity of the body, form violations of the immune status, and changes in the local immunity in the oral cavity stimulate the development of pathogenic microflora. Our research is devoted to the study of changes in periodontal tissues in children living in ecologically favorable conditions and in ecologically polluted Precarpathian regions, a comparative characterization of changes in periodontium, a treatment plan for chronic catarrhal gingivitis, supplemented with chlorhexidine dentin and quercetin. According to the results of many researches, 0.12% chlorhexidine solution provides a pronounced antimicrobial effect on pathogenic microflora; and under the action of quercetin there is a stabilization of the antioxidant system, improvement of peripheral blood circulation, improvement of rheological properties of the patient's blood. We examined 60 patients with chronic catarrhal gingivitis living in environmentally stable conditions (Ivano-Frankivsk) (1 group), 15 people with intact periodontium and 60 children with chronic catarrhal gingivitis living in contaminated Precarpathian regions (Burshtyn) (group 2). All patients were treated with a set of therapeutic measures, which are recommended for the conventional treatment of patients with chronic catarrhal gingivitis. In groups 1a and 2a, patients were treated with chlorhexidine, calendula and chamomile infusion and 1% mefenamine paste. In groups 1b and 2b we applied a quercetin gel on the mucous membrane of the patient’s gums under an individual dental-gingival cap. For the purpose of comparative characterization of the two groups, an index assessment of the condition of periodontal tissues, study of rheographic parameters of periodontal tissues and radiographic characteristics of jaw bone tissue were performed. The results of the research showed a significant difference between clinical and laboratory parameters in patients with chronic catarrhal gingivitis living in environmentally friendly conditions compared with persons living permanently in ecologically polluted Precarpathian regions (p

The aim of this review article is to identify and present an overview on the effects of different palatal expansion device on periodontium. Orthodontic treatment is a popular option for adolescents and adult patients for achieving better alignment of teeth and better esthetics. Palatal expansion technique is useful in treatment of patients’ malocclusion. With newer palatal expansion devices, patient’s malocclusion can be treated in better way. It is important to understand that oral hygiene plays an important role in the maintenance of periodontal health during orthodontic treatment. In adult patients, regular monitoring regarding the periodontal health should be performed prior to and during orthodontic treatment.

Extrusive tooth movements are an important resource in orthodontic treatment and allow the manipulation of teeth and periodontal tissues. They can be performed quickly or slowly, depending on the patient’s need. Rapid extrusion is indicated for cases in need of prosthetic preparation or restoration, where the bone and gingival tissues are intact, such as horizontal and oblique fractures, coronary or external root resorption, iatrogenic perforations (trepanations) and the presence of subgingival caries. The aim of this study is to describe the treatment of a patient who had a coronary fracture of the right upper central incisor, with a limit located 1 mm above the level of the bone crest. Rapid orthodontic extrusion was performed, to restore biologic distances and allow the preparation for prosthesis. It started with partial differentiated bonding of a fixed orthodontic appliance to the upper arch, to allow for a 3mm orthodontic extrusion. At the end of the extrusion, periodontal surgery was performed to increase the clinical crown and endodontic treatment. After these procedures, the case was concluded with the fixed prosthesis installation. The realization of an integrated planning allowed the restoration of aesthetics, with preservation of the functional periodontal limits for the patient. Keywords: Tooth Movement Techniques. Orthodontic Extrusion. Crown Lengthening. ResumoOs movimentos dentários extrusivos constituem um recurso importante no tratamento ortodôntico e permitem a manipulação dos dentes e dos tecidos periodontais. Eles podem ser realizados de forma rápida ou lenta, dependendo da necessidade do paciente. A extrusão rápida está indicada para casos com necessidade de preparo protético ou restauração, onde os tecidos ósseo e gengival encontram-se íntegros, tais como fraturas horizontais e oblíquas, reabsorções coronárias ou radiculares externas, perfurações iatrogências (trepanações) e presença de cárie subgengival. O objetivo deste trabalho é descrever o tratamento de uma paciente que apresentava fratura coronária do incisivo central superior direito, com limite localizado 1mm acima do nível da crista óssea. Realizou-se extrusão ortodôntica rápida, com a finalidade de restabelecer as distâncias biológicas e permitir o preparo para prótese. Iniciou-se com colagem diferenciada parcial de aparelho ortodôntico fixo no arco superior, para permitir a extrusão ortodôntica de 3mm. Ao término da extrusão, realizou-se cirurgia periodontal para aumento da coroa clínica e tratamento endodôntico. Após esses procedimentos, o caso foi finalizado com a instalação da prótese fixa. A realização de um planejamento integrado permitiu o restabelecimento da estética, com preservação dos limites periodontais funcionais para a paciente.Palavras-chave: Técnicas de Movimentação Dentária. Extrusão Ortodôntica. Aumento da Coroa Clínica.

While definitive evidence awaits, cannabis is emerging as a likely risk factor for periodontal tissue destruction. The mechanisms that underlie potential cannabis-induced or cannabis-enhanced periodontal diseases, however, remain to be elucidated. Herein, we 1) examine insights obtained from the endocannabinoid system, 2) summarize animal models of exposure to cannabinoid receptor agonists and antagonists, 3) review the evidence suggesting that cannabis and cannabis-derived molecules exert a profound influence on components of the oral microbiome, and 4) assess studies indicating that marijuana and phytocannabinoids compromise the immune response to plaque. Furthermore, we address how knowledge of cannabinoid influences in the oral cavity may be exploited to provide potential novel periodontal therapeutics, while recognizing that such medicinal approaches may be most appropriate for nonhabitual marijuana users. The suspected increase in susceptibility to periodontitis in marijuana users is multifaceted, and it is clear that we are only beginning to understand the complex toxicological, cellular, and microbial interactions involved. With marijuana consumption increasing across all societal demographics, periodontal complications of use may represent a significant, growing oral health concern. In preparation, an enhanced research response would seem appropriate.

Aim. To study scientific literature which covers the onset and course of major periodontal diseases and possible methods of body modulation for such patients. To analyze the information provided by the modern scientific researches of periodontal diseases pathogenesis and treatment results to outline prospective directions of therapeutic influence on the patient’s organism. Conclusions. The pathogenesis of gingivitis and periodontitis is determined by some factors, the main of which are local, microorganisms in particular, that are counteracted by the immune system. Periodontal tissue is in close contact with the organs and systems of the body. Their diseases significantly affect the course, treatment and prevention of periodontitis. This requires a multifaceted approach and the choice of the most effective drugs. The primary strategy of preventing gingivitis is to eliminate the attachment of periodontal pathogens to the epithelium and surface of the teeth to prevent the penetration of microorganisms and their toxins through natural protective barriers. It is more important to resort to biological approaches using probiotics, as well as other natural media in order to prevent dysbiosis, long-term treatment, and maintenance therapy. The concept of vaccination against periodontitis is now being elaborated. Correction of the pathological process in the periodontium is considered in the context of the overall biological response of the organism. Therefore, the assessment of the role of its regulators reveals not only the features of local mechanisms, modulation pathways, but also the choice of effective drugs. Prospects of new approaches and introduction of highly effective drugs are critically analyzed.

Objective To assess the rate of tooth movement and the periodontal tissue status over a period of 90 days with and without micro-osteoperforation (MOP). Method Thirty-three adults of the 19 to 25 age group undergoing labial fixed orthodontic treatment with bilateral maxillary first premolar extraction, requiring individual canine retraction as a part of the treatment plan, were recruited for this split-mouth randomized clinical trial. While performing micro-implant–assisted canine retraction in the maxillary arch, the experimental side received three MOPs each on the mesial and distal aspects of the canine root. The amount of tooth movement was measured clinically at every 15 days interval for 90 days; the periodontal status was assessed clinically (probing depth, relative attachment level) and tomographically (canine root length, alveolar bone level) at the 1st day and 90th day of retraction. The data were subjected to appropriate statistical analyses. Results A statistically significant difference in tooth movement on the MOP side was observed in the first 45 days, amounting to 1.5 times more than that of the control side. However, during 45 to 90 days, the difference in the rate of tooth movement between the sides was not statistically significant. Changes in periodontal variables were also insignificant between the sides except for the distal alveolar bone level. Conclusion An increase in the rate of tooth movement can be achieved without any periodontal adverse effects in the first 45 days of the MOP procedure. The effectiveness of the MOP procedure on the rate of tooth movement gradually declined thereafter. Trial Registration: CTRI/2019/07/020403.

Guided tissue regeneration (GTR) has been proven to promote attachment and regeneration of periodontal tissue. However, there is a 20 to 40% incidence of attachment loss on regenerated attachments reported in the literature. To my knowledge, this is the first case report on a second attempt in GTR on a previous successful grafted site with clinical attachment loss. A healthy 17-year-old Chinese male patient had GTR performed with xenograft particles and bovine resorbable membrane on his root-canal treated, fused upper right lateral incisor and upper right canine (#12-#13) in 2007. Probing depth on the mid-palatal region of #12-#13 was reduced to 4 mm and maintained for the next 4 years. But in the fifth year, probing depth increased to 11 mm with no endodontic symptoms, and a second attempt of GTR using the same materials was carried out. The probing depth at the surgical site was reduced to 4 mm and successfully maintained for another 5 years. Irregular maintenance and the presence of plaque retentive factor could have caused the clinical attachment loss on #12-#13. This case shows it is possible to attempt GTR on a previous successfully grafted site. GTR did not increase tissue resistance against periodontal breakdown. Hence, proper maintenance planning for GTR sites is important to prevent periodontal breakdown.

The aimof the research:to experimentally study at the histological and morphological level the degree of the corrective effect of bacterial lysate of the disturbed non-specific defense of the body on the model of periodontitis based on the Central Research Laboratory of the National University of Pharmacy. Materials and methods:prospective study has been conducted on experimental periodontitis in 42 rats for 90 days. The animals were treated with «Respibron» and the reference drug «Imudon». Histological and morphometric studies were carried out according to standard methods. Micropreparations were viewed under a Granum DCM 310 digital video camera. All interventions and euthanasia of animals were carried out in compliance with the European principles. Results: by the end of 90 days of experimental periodontitis at the local level in the homogenate of animal gum tissue compensatory mechanisms are depleted and differed from the norm by 397 times. The dynamics of the studied morphometric and histological parameters of "Respibron" was similar to the "Imudon", but the magnitude of destruction was less pronounced and differed at the end of the experiment by 17.2 times in comparison with the intact control, and in the control group the results improved by 23.1 times. Conclusion: the obtained data from the study indicate a high decompensation of experimental periodontitis. It is characterized by the formation of periodontal pockets and inflammatory bone loss. The magnitude of destruction differed from the norm by 397 times. Applying of bacterial lysates led to the compensation of bacterial dysbiosis, restoration of the tissues of paradont. The therapeutic effect of "Respibron" can be assessed as more powerful in comparison with "Imudon" in terms of the studied morphometric and histological parameters: the magnitude of improvement "Respibron" was 3.72 times higher than the indicators of "Imudon". We should continue the study of experimental periodontitis as mechanisms of development, protection, and restoration of tissues under conditions of pharmacological correction by bacterial lysate "Respibron"

The clinicians face various challenges in trying to find the optimum working length during root canal treatment. Unless proper biomechanical preparation up to the apical limit is done, reinfection of the canal occurs. Apical reference point must be measured with at most care as to avoid any damage to the surrounding periodontal tissues during the treatment.

Honey is a sweet, viscous natural substance made from flower nectar by bees. Honey has been used not only as a nutritional source but also for wound healing and to reduce tissue inflammation. Nevertheless, the use of honey in the treatment of periodontitis is not well established. This study aimed to evaluate the effect of locally delivered Tualang honey on periodontal tissue healing by a randomized controlled split-mouth clinical trial involving 20 chronic periodontitis patients with a periodontal pocket depth of ? 5 mm. Each site was randomly treated either by scaling and root debridement alone (Control Group) or scaling and root debridement with locally delivered Tualang honey (Test Group). Assessment of probing pocket depth (PPD) and clinical attachment level (CAL) was recorded at baseline and after 6 weeks interval. Gingival crevicular fluid samples were collected from treated pockets at baseline and along with periodontal reassessment to evaluate the level of Matrix Metalloproteinase 8 (MMP-8) and Osteoprotegerin (OPG). Data were analysed by using Wilcoxon Signed Rank Test and Paired Sample t-Test. PPD and CAL were significantly improved after the 6 weeks review (P=0.001) in both groups. However, there was no significant difference in the changes of the PPD, CAL, MMP-8 and OPG levels after the 6 weeks review and in between the groups. In conclusion, within the limitations of this study, the effect of locally delivered Tualang honey on periodontal tissue healing is not evident. Nevertheless, all pockets achieved good periodontal healing.

espanolAntecedentes: Los procedimientos quirurgicos regenerativos en periodoncia e implantes son realizados de manera analogica con el uso de imagenes en dos dimensiones. El uso de modelos y reconstrucciones 3D asi como la impresion 3D, se encuentra en desarrollo para el mejoramiento de las tecnicas regenerativas quirurgicas. Objetivo: Identificar alternativas tecnologicas 3D que pueden ser utiles en la planificacion y practica de cirugia regenerativa en periodoncia e implantologia, mediante una revision de literatura. Metodologia: Primero la busqueda de la informacion se realizo en bases de datos (PubMed, Science Direct y Google Scholar), subsecuente la lectura y analisis de articulos de investigacion, para pasar a un proceso de seleccion estricto. Resultados: Existe evidencia del uso de tecnologia 3D para impresion de dispositivos que ayudan a mejorar la regeneracion de tejidos periodontales, asi como, la preservacion de rebordes alveolares. Se sustenta en la aplicacion de diferentes componentes altamente compatibles con celulas vivas, y se predice su futuro uso en cirugia regenerativa en las areas de periodoncia e implantologia. EnglishBackground: Regenerative surgical procedures in periodontics and implants are performed traditionally in non digital pathway using two dimensional images. The use of three dimensional (3D) models and reconstructions as well as 3D printing is in development in order to improve regenerative surgical techniques. Objective: To identify 3D technological alternatives that can be useful in planning and practice of regenerative surgery in periodontology and implantology, through a literature review. Methodology: First the information search was carried out in scientific databases (PubMed, Science Direct and Google Scholar), followed by reading and analysis of research articles, to go through a strict selection process. Results: There is evidence of the use of 3D technology for printing devices that help to improve the regeneration of periodontal tissues, as well as the preservation of alveolar ridges. It is based on the application of different components highly compatible with living cells and it is predictable their use in regenerative surgery in areas of dental surgery as periodontics and implantology

Bone marrow mesenchymal stem cell-derived small extracellular vesicles (BMSC-sEVs) can be used as a potential cell-free strategy for periodontal tissue regeneration, and we aim to investigate the effect and possible mechanism of BMSC-sEV in periodontal tissue regeneration in this study. The BMSC-sEV was isolated by the Exosome Isolation™ reagent and identified by transmission electron microscopy, nanoparticle tracking analysis, and Western blotting. The human periodontal ligament cells (hPDLCs) were cocultured with BMSC-sEV

Essam Abdelalim Nassar,1 Naif Nasser Almasoud,1 Maher Sulaiman Al-Qurashi,2 Ahmed A Alsulaiman,1 Khalid Sadiaq Hassan3 1Department of Preventive Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia; 2Department of Microbiology, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, KSA, Saudi Arabia; 3Department of oral Medicine and Periodontology, College of Dentistry, Al-Azhar University, Assiut, EgyptCorrespondence: Essam Abdelalim NassarDepartment of Preventive Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Saudi ArabiaTel +966542941098Email e_abdelalim@yahoo.comPurpose: To compare the microbial flora, alkaline phosphatase (ALP) and interleukin-8 (IL-8) levels during orthodontic treatment with self-ligating brackets versus conventional brackets.Patients and methods: Forty patients were included in this study and distributed equally between two groups. One group received conventional edgewise brackets while the other group received DAMON self-ligating brackets. Periodontal parameters were recorded and biochemical and microbial analyses were conducted before bonding (baseline), and 14, 30, and 45 days after bonding. Paired t-test was performed to estimate the intergroup differences from the baseline at the various time points; unpaired t-test was used for intragroup comparisons.Results: A significantly higher prevalence of gram-negative as well as gram-positive microorganisms was found in the group with conventional brackets than that with self-ligating brackets. Alkaline phosphatase (ALP) activity and interleukin-8 (IL-8) levels were significantly higher in the gingival crevicular fluid of the group with conventional brackets than that with self-ligating brackets.Conclusion: The conventional bracket-ligature technique exhibited an increase in IL-8 levels, ALP activity, and microbial colonization compared with that of the self-ligature technique. Accordingly, self-ligating brackets are recommended in orthodontic patients to improve periodontal health and minimize damage outcomes of periodontal tissue interventions.Keywords: dentistry, orthodontics, alkaline phosphatase, jnterlukin-8, orthodontic tooth movement, periodontal parameters

Periodontal regeneration aims to retain teeth while restoring the attachment between the dental and periodontal structures. This goal is highly achievable with the emerging technologies and a deeper understanding of biology, material science, cell function, and molecular regulation of tissue responses. A holistic approach is needed to connect these knowledge under an innovative perspective to accomplish the regeneration of periodontal tissues.

The purpose of our study was to increase the level of treatment of periodontal diseases in children with rheumatic diseases through the use of injections of homeopathic drugs in complex treatment. To do this, they conducted a dental examination of children with rheumatological diseases, determined the degree of damage to periodontal tissues using the indices PMA, GI, CPITN and compared with the control group of children. The relationship between inflammatory processes in in periodontal tissues and rheumatic diseases in children was revealed and the effectiveness of homeopathic drugs in the complex treatment of periodontal tissues was assessed.

Heat shock protein 60 (Hsp60) is considered as one of the possible autoantigens involved in the pathogenesis of a number of chronic diseases including periodontal diseases. The application of hyaluronic acid or hyaluronan (HA) in the treatment of periodontitis has been evaluated in several clinical trials, however, the effect of hyaluronic acid on heat shock protein 60 level in periodontal soft tissues has not been studied. The aim of this work was to evaluate the effect of HA injections on levels of Hsp60 in periodontal tissue of the rats. Samples of periodontal tissue of mandibular incisors of adult male Wistar rats at 10-12 months of age were investigated. Rats were distributed into the control group and the periodontitis group. Visual manifestations of hyperemia of the gums around the incisors were the criterion for selecting animals into the periodontitis group. There were two subgroups in the control group: intact rats (I); intact rats after HA “hyaDENT BG” 1.0MDa (BioScience GmbH, Germany) treatment (I+“G-1.0”). There were four subgroups in the periodontitis group: rats with periodontitis (P); rats with periodontitis after HA “hyaDENT BG” 1.0 MDa (BioScience GmbH, Germany) treatment (P+“G-1.0”); rats with periodontitis after HA “SERTOBEC” 2.4 MDa (S.C. Rompharm Company S.R.L., Romania) treatment (P+“S-2.4”); rats with periodontitis after HA “SERTOBEC Tendon” 2.4 МDа (S.C. Rompharm Company S.R.L., Romania) treatment (P+“ST-2.4”). There were three animals in each subgroup. Rats were injected 0.05 ml HA in the area of alveolar processus of central incisors once a week, three times. Levels of Hsp60 intotal lysates of periodontal tissue were tested by Western blotting method before and after the treatment with HA (one month after the last injection). There was no significant difference between levels of Hsp60 in total lysates of periodontal tissue of intact rats and rats with periodontitis before treatment (p>0.05). Rats with periodontitis showed decreased inflammation in the periodontal tissue after treatment with HA with different molecular weight. Intact rats and rats with periodontitis which were treated with HA “hyaDENT BG” 1.0MDa showed reduced levels of Hsp60 in total lysates of periodontal tissue comparatively with levels of Hsp60 before treatment (by 15.4 and 10.7 times respectively, p

Although guided tissue regeneration (GTR) is a useful tool for regenerating lost tissue as bone and periodontal tissue, a biocompatible membrane capable of regenerating large defects has yet to be discovered. This study aimed to characterize the physicochemical properties and biological compatibility of polycaprolactone (PCL) membranes associated with or without nanostructured hydroxyapatite (HA) (PCL/HA) and Zn-doped HA (PCL/ZnHA), produced by electrospinning. PCL, PCL/HA, and PCL/ZnHA were characterized by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and differential scanning calorimetry (DSC). Nanoparticles of HA or ZnHA were homogeneously distributed and dispersed inside the PCL fibers, which decreased the fiber thickness. At 1 wt% of HA or ZnHA, these nanoparticles acted as nucleating agents. Moreover, HA and ZnHA increased the onset of the degradation temperature and thermal stability of the electrospun membrane. All tested membranes showed no cytotoxicity and allowed murine pre-osteoblast adhesion and spreading; however, higher concentrations of PCL/ZnHA showed less cells and an irregular cell morphology compared to PCL and PCL/HA. This article presents a cytocompatible, electrospun, nanocomposite membrane with a novel morphology and physicochemical properties that make it eligible as a scaffold for GTR.

Kravchenko L. S., Koval S. N., Plotnikova V. G., Bila N. F., Zherebko A. N., Ivanova Ya. I. Influence of new dental elixir on rats periodontal tissues with modeling of metabolic syndrome. Journal of Education, Health and Sport. 2021;11(05): 138-146. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2021.11.05.014 https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2021.11.05.014 https://zenodo.org/record/4907573 The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019. © The Authors 2021; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 15.05.2021. Revised: 21.05.2021. Accepted: 30.05.2021. INFLUENCE OF NEW DENTAL ELIXIR ON RATS PERIODONTAL TISSUES WITH MODELING OF METABOLIC SYNDROME L. S. Kravchenko, S. N. Koval, V. G. Plotnikova, N. F. Bila, A. N. Zherebko, Ya. I. Ivanova Odessa National Medical University, Odessa, Ukraine Kravchenko L. S. https://orcid.org/0000-0003-0677-8713 Abstract Aim: to study the changes in the periodontal tissues of rats with simulation of metabolic syndrome of alimentary genesis and to determine a possibility of their correction with the new dental elixir. Materials and methods: Biochemical changes in the blood serum, liver and gingival tissue were examined during simulation of the metabolic syndrome of alimentary genesis in experiment on 28 white male rats of reproductive age with an average body weight (280±14)g. Results. It was found that keeping animals for ten weeks on a diet high in saturated fats and carbohydrates led to increase in body weight and in level of biochemical markers: insulin resistance, hyperglycemia, inflammation, both at the level of the body and locally in the periodontal tissues. Local application of the new dental elixir in the form of applications to rats gums against the background of metabolic syndrome reduced the level of triglycerides, had an anti-inflammatory and antioxidant effect, reducing contamination by pathogenic microflora (urease activity), lipid peroxidation activity (malonic dialdehyde content), activating locally in the periodontal tissues the antioxidant defense enzyme and nonspecific immunity — lysozyme. Conclusions. The use of the new oral hygiene product in experimental metabolic syndrome improved metabolism, reduced inflammation, presenting periodontal protective properties, which allows to offer it for clinical testing in patients with periodontitis against a background of metabolic syndrome. Key words: inflammation; treatment; metabolic syndrome; gingival tissue; dental elixir.

У статі наведено результати визначення клініко-лабораторних особливостей перебігу генералізовано-го пародонтиту І-ІІ ступеня у жінок після хіміоте-рапії з приводу рака молочної залози у порівнянні з пе-ребігом генералізованого пародонтиту аналогічного ступеня у жінок без онкопатології. Показано, що у жінок з раком молочної залози, що перенесли хіміотерапію, спостерігається превалю-вання дистрофічних змін в пародонті (ПІ Russel збі-льшений на 25,9 % у порівнянні з жінками з ГП без онкопатології, рецесія ясен – в 1,7 разів, втрата епі-теліального прикріплення – на 69,7 %), зафіксовано поганий гігієнічний рівень (збільшення гігієнічних ін-дексів на 33,9-50,6 %), гіпосалівацію ІІІ ступеня та значне зниження неспецифічного захисту порожнини рота. Показники запалення (симптоматичного гінгі-віту) у пацієнток цієї категорії збільшені, але досто-вірно не відрізняються від аналогічних показників у жінок з генералізованим пародонтитом без онкопа-тології.

BACKGROUND It is of outmost importance to know the effects of nickel on a cellular level. The objective of this systematic review was to analyse the factors affecting nickel release, the amount of nickel being released in commercially available NiTi wires and to also analyse the blood / periodontal evaluation after orthodontic treatment in conventional and nickel free brackets. METHODS For this systematic review, relevant articles were searched in PubMed, MedLine, Cochrane, EMBASE and Google Scholar databases, along with a complimentary manual search of all orthodontic journals from January 1980 till the year 2019 December. The study included in vitro and in vivo studies. 14 articles were included in this systematic review. RESULTS 160 articles were obtained initially. 41 were obtained by a manual search. 186 articles were excluded based on the inclusion criteria and 14 articles were found to be eligible. CONCLUSION This systematic review shows that nickel solution at a minimal concentration could damage human gingival fibroblast. As the carcinogenic effect of nickel has been already confirmed, other alloys that do not contain the heavy metal nickel can be used or standardization of the amount of nickel leach out from the appliance should be considered. KEY WORDS NiTi, Nickel Release, Periodontal Tissue or Periodontium, Cytotoxicity, Orthodontics

The polyethiology of periodontal diseases introduces certain difficulties in the diagnosis and adequate selection of treatment methods and dictates the conditions under which the complexity and individual approach are the main conditions for successful treatment. Therefore, it is very important to timely diagnose and correctly determine the prognosis of the disease, which directly depends on the level of professional knowledge of dentists. The aim of the study was to analyze the level of competence of dentists on the methods of diagnosis and classification of periodontal tissue diseases in patients in Bishkek. Questioning of respondents — 87, statistical. For the study, a questionnaire “Dentist’s awareness of modern principles of diagnosis and treatment of periodontal tissues” was developed, containing 14 questions that reveal the ideas of dentists-therapists about modern principles of classification, diagnosis and methods of treatment of inflammatory diseases of periodontal tissues. According to the results of the survey, low competence in the diagnosis of periodontal diseases was revealed: 34.5% of the respondents are well aware of classification issues; adequate evaluation indices for assessing the hygienic state of the oral cavity and inflammatory changes in the tissues of the gums are used only in 20.7% and 77.0% of cases, respectively; laboratory and diagnostic research methods are used only by 59.8%. The use of a large number of diagnostic and prognostic indices in periodontics makes it possible to objectively control the dynamics of the disease, assess the depth and prevalence of the pathological process, carry out mathematical processing of the results obtained and compare the effectiveness of various methods of treating periodontitis.

Inflammatory periodontal diseases are a serious medical and social problem and the effectiveness of the provision of medical and preventive care to patients is determined by a full diagnosis of the disease, a competent selection of methods of treatment and rehabilitation, a reasonable combination of therapeutic, surgical, orthopedic and physiotherapeutic measures. Complexity and an individual approach are the main conditions for successful treatment, which directly depends on the level of professional knowledge of dentists. The aim of the study was to analyze the level of competence of dentists on the methods of complex treatment and rehabilitation of patients with inflammatory diseases of periodontal tissues in Bishkek. Questioning of respondents — 87, statistical. For the study, a questionnaire “Dentist’s Awareness of Modern Principles of Diagnosis and Treatment of Periodontal Tissues” was developed, containing 14 questions that reveal the ideas of dentists-therapists about modern principles of classification, diagnosis and treatment of inflammatory diseases of periodontal tissues. According to the results of the questionnaire, low competence in treatment issues was revealed: 88.5% of the training at the advanced training courses took place on average 11.5±2.9 years ago; 58.6% (51/87) of respondents did not have a qualification category; Assessment of knowledge of the main stages of complex treatment — 66.7% did not give an answer, and 33.3% (29/87) noted various options for treatment stages and their combination. Evaluation of AMP use: 57.5% indicated metronidazole, 55.2% β-lactam antibiotics — amoxicillin and amoxicillin, then — lincomycin, erythromycin and chloramphenicol. Inappropriately prescribed: chloramphenicol, the frequency of administration of which should be 4 times a day; erythromycin — 3 to 4 times a day; lincomycin — 3-4 times a day, amoxicillin in 76.4% of cases, it was also prescribed irrationally, the recommended one — 3 times a day. In 50.6% of cases, analgesics and fluoride and calcium preparations were correctly used for symptomatic therapy, 49.4% of cases indicated the need to remove dental plaque.

Introdução: Devido à crescente demanda estética, a odontologia restauradora se renova na intenção de alcançar melhores resultados na harmonia do sorriso. Entretanto, a atenção deve voltar-se também aos tecidos periodontais, visto que há uma integração entre material restaurador e os tecidos. Objetivo: Descrever e discutir as etapas clínicas para a confecção de laminados cerâmicos, atentando-se a preparos minimamente invasivos, contorno cervical e fatores que podem intervir na longevidade do procedimento. Relato de caso: Paciente do sexo masculino, 28 anos de idade, compareceu a Associação Brasileira de Odontologia – ABO, seção de Alagoas, relatando insatisfação em relação ao tamanho dos seus dentes. Os arcos superior e inferior foram moldados para a obtenção dos modelos de estudo e enceramento diagnóstico. Ao início do tratamento foi orientado ao paciente clareamento dental pela técnica combinada. O preparo dos dentes foi guiado por uma matriz de silicone, sendo estes uniformes e conservadores, seguido de moldagem, fase laboratorial e cimentação final. Finalizado o caso foi realizado o ajuste oclusal solicitando ao paciente que realizasse movimentos de lateralidade e protrusão. Conclusões: A técnica do preparo influência na longevidade dos laminados cerâmicos, por relacioanar-se à adesão e a saúde periodontal. O preparo deve ser mínino e limitado ao esmalte dental sempre que possível, determinando assim um maior e melhor prognóstico.

BACKGROUND Periodontitis is an inflammatory disease of periodontium. The inflamed and ulcerated epithelium allows the entry of oral microorganisms, bacterial endotoxins, proinflammatory immune mediators, like Interleukin -1 (IL-1), Interleukin-6 (IL-6), tumour necrosis factor (TNF-α) and prostaglandin E2 (PGE2) into the systemic circulation, which may produce systemic effects. The present study was conducted to evaluate the effect of periodontal inflammation on haematological and liver function parameters. METHODS The clinical study was conducted among 30 patients with periodontal inflammation. Clinical parameters [plaque index (PI), gingival index (GI), probing pocket depth (PPD)], haematological [RBC count, haemoglobin (Hb) concentration, total leukocyte count and differential leukocyte count] and liver function parameters [alanine aminotransferase (ALT), aspartate aminotransferase (AST) & alkaline phosphatase (ALP)] were recorded at baseline and after 1 month of scaling and root planning (SRP). RESULTS There was significant improvement in clinical parameters (P-value < 0.001), haematological parameters (P-value < 0.001), except eosinophils (P = 0.303) and basophils (P = 0.860). Changes in liver function test (LFT) parameters were found to be significant (P < 0.05) after SRP. CONCLUSIONS There is an association between periodontal inflammation and systemic parameters. KEY WORDS Periodontal Inflammation, Haematological, LFT.

Introduction: The treatment of periodontal disease is considered complex, including, in the first phase, a non-surgical therapy, and then, eventually, a surgical therapy. The modified Widman flap presents itself as a technique that allows a decrease in the probing depth of the pockets with maximum preservation of the periodontal tissue, indicating when aesthetics is an important factor. Descrition of the case report: A 53-year-old female patient was diagnosed with periodontitis stage IV grade B. Non-surgical periodontal treatment was started, using root planing. In the revaluation consultation, it was found that the depth of probing of the pockets did not reach normality. Treatment was continued using the Widman flap periodontal surgical technique modified in the anterior sextant. Conclusions: The modified Widman flap surgical technique proved to be a treatment option, achieving stability and periodontal health.

Purpose/Aim of the study: The ultimate goal of periodontal treatment is to regenerate the lost periodontal tissues. The interest in nanomaterials in dentistry is growing rapidly and has focused on ...

Objective. To study the indicators of the dental status in professional athletes. Materials and methods. The object of the study was 200 professional female athletes representing various kinds of sport and 200 practically healthy female individuals who are not involved in sports. The total sports experience of the subjects was on average 1315 years. The prevalence of periodontal diseases among female athletes was determined using the CPITN index. The prevalence and intensity of caries was determined by the CPI index. The hygienic state of the oral cavity was determined using the oral hygiene efficiency index (PHP). In the main group, patients were administered applications of a drug based on propolis and vegetable oil for two weeks; in the comparison group, the traditional antiseptic, chlorhexidine bigluconate, was used for the same period. Results. In the control group, the prevalence of chronic generalized gingivitis was on average two times less than in the main group, in which the value of this indicator varied within 50 %. Among all the studied diseases of the oral cavity in athletes in general, the most essential and significant differences were observed in relation to the prevalence of dental caries and its complications, which were more common in the group of athletes than in the control group 95.0 1.54 %, versus 89.5 2.17 %, respectively (p 0.001). The need for treatment of moderate and severe inflammatory periodontal diseases in the main group was approximately 1.5 times higher than in the control group (77.0 2.98 vs. 42.5 3.50 %, p 0.001), and for gingivitis almost 2 times higher than in the control group (61.5 3.44 vs. 39.0 3.45 %, p 0.001). The results of the study showed that among the oral diseases in sportswomen, the most essential and significant differences were observed in the prevalence of dental caries and its complications. The intensity of periodontal tissue damage over the entire period of sports career in athletes of the older age group corresponded to moderate and severe periodontitis. The high percentage of cases of major dental diseases detected among female athletes indicates their high need for periodontal care. Conclusions. Regular use of a biologically neutral propolis-based preparation for preventive purposes leads to a relatively more pronounced improvement in laboratory and clinical indicators of the oral cavity status.

Aim: To evaluate the periodontal status before and after orthodontic treatment and to analyze the confounding factors associated with it. Materials and Methods: The present retrospective study analyzed pre- and posttreatment records of a total of 60 patients. Intraoral digital photographs before and after the orthodontic treatment were used to measure the following three periodontal variables: (a) width of the keratinized gingiva, (b) gingival recession, and (c) the status of interdental papilla. The ImageJ 46 software imaging program was used to perform the required measurements. The clinical data were then analyzed in an association with the participants’ demographic data, the type of orthodontic tooth movement, and other confounding factors. The data obtained were manually entered into the statistical package and analyzed using a significance level set at P < .05. Descriptive statistics, paired t-tests, and Pearson’s correlation analysis were used to identify differences among the studied variables. Results: Among the 60 patients, the majority (66.7%) were females. Most cases of the treated malocclusion were Class II, and most patients underwent intrusion and retrusion movements. The width of keratinized gingiva increased for most sites of upper teeth. In contrast, the lower teeth showed a mix of improvement and compromised width of keratinized gingiva. As a measure of gingival recession, the difference of clinical crown height revealed the tendency toward a reduced clinical crown height for most sites. Upper left lateral incisors and canines showed significant values with regard to keratinized gingival width measurements and tooth movements such as extrusion and intrusion movements. Likewise, with regard to the clinical crown height, upper right central incisors showed significant differences when correlated with the interincisal angle, lower incisors to the mandibular plane, and upper incisors to the NA line. The upper lateral incisors also showed significant correlations to certain cephalometric measurements. Conclusions: The orthodontic treatment was found to exert a significantly positive impact on the surrounding periodontium, particularly in the upper canine areas. Likewise, various types of tooth movement were found to positively affect the periodontium.

Hypophosphatasia (HPP) is an inherited skeletal disorder that features early exfoliation of primary teeth as a major dental manifestation. A 6Y4M Japanese girl diagnosed with childhood HPP came to our clinic. Deep probing depth for all teeth and severe mobility of primary incisors were observed. At 7Y1M, the mandibular central incisors received a traumatic injury, with no treatment performed and slight mobility observed thereafter. Such traumatic injuries were repeated, thus mouthguards (MGs) to protect weak periodontal tissue were applied, which helped to maintain stable conditions. Use of a MG may be helpful for HPP patients.

Objective This study aimed to investigate the levels of trefoil factor family (TFF)-1, TFF-3 and interleukin (IL)-1 beta in gingival crevicular fluid (GCF), saliva and serum of patients with gingivitis, stage 3 periodontitis and healthy individuals. Materials and Methods A total of 100 individuals consisting of 25 periodontally healthy, 25 gingivitis and 50 stage 3 periodontitis, were enrolled in the study. Clinical periodontal examinations were recorded and GCF, saliva and serum samples were obtained. TFF-1, TFF-3 and IL-1 beta were measured by ELISA. Results TFF-1 and TFF-3 levels in both GCF, saliva and serum were higher in periodontitis patients than healthy controls (p < .001) and gingivitis group (p < .01). The levels of these peptides in all biofluids were similar between gingivitis and healthy control groups (p > .05). GCF, saliva and serum IL-1 beta levels were also higher in periodontitis patients than the controls (p < .01). Periodontitis patients had elevated GCF and saliva IL-beta levels than gingivitis group (p < .001). Conclusion Elevated TFF-1 and TFF-3 levels both locally and systemically in periodontitis in parallel to increased IL-1 beta levels might suggest that these peptides are involved in host response during the periodontal tissue destruction., TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [ARDEB-118S216], TUBITAK, Grant/Award Number: ARDEB-118S216

The aim of the study was to study the dental health of university students in Samarkand and assess the impact of organizational, medical and social risk factors on it. A comprehensive examination of the dental health of students in the city of Samarkand was carried out. The research took place between Institutions of Higher Education. The total number of students is 574. The following universities participated: Samarkand Medical Institute and Samarkand State University among which there were 193 students of the 1st year, 175 students of the 2nd year, 206 students of the 3rd year, respectively. The prevalence of dental caries was revealed, which was 91.87 ± 1.30%. ... The intensity of the carious process was 5.31 ± 0.14 teeth at 8.34 ± 0.26 of the surface, signs of periodontal tissue diseases were revealed with an intensity of 3.88 ± 0.06 segment. 53.05 ± 2.37% of students had dentoalveolar anomalies and deformities

The dental gingivae are a unique part of the oral anatomy and an integral part of the periodontal tissues. Although the vast majority of abnormalities affecting the gingival tissues are due to a simple inflammatory reaction directly related to the presence of dental plaque, a range of non-plaque-related conditions also occur due to either local or systemic factors. Such factors include developmental abnormalities, the presence of malignancy and manifestations of underlying systemic conditions. Recognition and diagnosis of non-plaque-related gingival disease is essential for comprehensive dental health care. CPD/Clinical Relevance: This paper provides a review of the spectrum of non-plaque-related conditions that can affect the dental gingivae.

In the process of orthodontic treatment, the balance between the modeling of alveolar bone and the mechanical stress exerted by the appliance is key to the effective movement of orthodontic teeth. Alveolar bone modeling involves many regulatory factors, and microRNAs (miRNAs), as posttranscriptional regulatory factors, play an important role in the occurrence of bone modeling. As an important member of the miRNA family, miRNA-21 promotes the differentiation of periodontal ligament stem cells into osteoblasts and plays an important role in maintaining bone balance and preventing bone resorption as a regulator of osteoclast formation and a promoter of osteoclast differentiation. A literature review showed that miRNA-21 can regulate osteoclast function and promote bone resorption through programmed cell death 4 (PDCD4), phosphate and tension homology deleted on chromosome ten (PTEN), receptor activator of nuclear factor-κB ligand (RANKL) and osteoprotegerin (OPG). MiRNA-21 is highly sensitive to external mechanical stress in the process of orthodontic tooth movement. After orthodontic force is applied, miRNA-21 can promote osteoclast formation and accelerate orthodontic movement; through targeted regulation of periodontal ligament associated protein-1 (PLAP-1), it can regulate periodontal ligament remodeling in the late stage of tooth movement and improve the potential of tooth movement. In addition, miRNA-21 mediates orthodontic tooth movement (OTM) and alveolar bone remodeling in the periodontal inflammatory microenvironment. miRNA-21 can upregulate the expression of hypoxia-inducible factor-1α (HIF-1α) in periodontal ligament stem cells in a hypoxic environment. It can promote the expression of osteogenic markers, such as osteopontin (OPN), osteocalcin (OCN), alkaline phosphatase (ALP) and runt-related transcription factor 2 (Runx2), and promote osteogenic differentiation during orthodontic tooth movement.

The extraction of impacted lower third molars (ILTM) is one of the most common procedures in oral-maxillofacial surgery. Being adjacent to lower second molars, most impacted lower third molars often lead to distal periodontal defects of adjacent second molars. Several symptoms may occur after extraction, such as periodontal pocket formation, loss of attachment, alveolar bone loss and even looseness of second molar resulting in extraction. The distal periodontal defects of second molars are affected by many factors, including periodontal conditions, age, impacted type of third molars, and intraoperative operations. At present, several studies have suggested that dentists can reduce the risk of periodontal defects of the second molar after ILTM extraction through preoperative evaluation, reasonable selection of flap design, extraction instruments and suture type, and necessary postoperative interventions. This review summarizes the research progress on the influence factors, interventions methods and some limitations of distal periodontal defects of adjacent second molar after extraction of impacted mandibular third molars, with the aim of opening up future directions for studying effects of ILTM extraction on periodontal tissue of the adjacent second molar.

There are numerous domestic and foreign studies of periodontal disease in pregnant women, but there are still a number of unresolved issues. The data about the development of periodontal tissue diseases in pregnant women in terms of age is not enough. The purpose of this work was to study the development of periodontal disease in pregnant women in different age groups. Material and methods. A survey of 83 women in the ІІ and ІІІ trimesters of pregnancy, aged 19-45 years, was divided into three groups according to age. Group I (34 people) included pregnant women aged 19-25 years, group II (29 people) had pregnant women aged 26-35 years, group III (20 people) comprised pregnant women aged 36-45 years. Periodontal and hygienic indices were determined to assess the clinical signs of periodontal disease (to assess the clinical signs of periodontal disease, periodontal and hygienic indices were determined (papillary-marginal-alveolar index, degree of bleeding gums, Schiller-Pisarev test, numerical value of Schiller-Pisarev test – Svrakov's number and simplified index of cavity hygiene). Results and discussion. The obtained results indicated a high level of periodontal disease in pregnant women. Periodontal disease was found in 74.7% of subjects. The study found a significant progression of periodontal tissue pathology in pregnant women over 26 years of age, indicating that women in this age group were at significant risk of periodontal disease. The obtained data indicated that the value of the bleeding index was the lowest in pregnant women with periodontal disease of group I, and the highest in women of group III. The value of the PMA index differed 1.3 times (p˂0.05) in pregnant women of groups I and II, 1.2 times (p˂0.05) in women of groups II and III, and 1.5 times (p˂0.05) in pregnant women of groups I and III. In addition, the likelihood of developing periodontal disease increased with age of women. Conclusion. The values of the Svrakov's number had a similar tendency to increase in age groups, as well as the results of the previously described indices. Hygiene in pregnant women of all ages who had periodontal disease was not very good. Thus, special attention should be paid to the timely diagnosis, treatment and prevention of periodontal disease in pregnant women, taking into account their age

The aim of periodontal regenerative therapy is to restore original architecture and function of lost periodontal tissues, as a result of trauma or following destructive periodontal diseases. This review includes the biological principles and efficacy and effectiveness of different biomaterials and factors affecting periodontal regeneration. Various human clinical trials showed a successful periodontal regeneration with different biomaterials. However, postoperative plaque control is one of the key factors influencing periodontal healing following regenerative periodontal therapy. A strong native regenerative potential of the periodontium can be conciliated by local and systemic factors. Keywords: Periodontal regeneration, Guided tissue regeneration, Bone graft/Bone substitutes.

Objective The aim of the research was to study the cellular composition of the gums in children of primary school age with normal body weight and overweight for further use of this data in the early diagnostics of periodontal diseases. Patients and methods Materials and methods: We examined 81 children aged from 6 to 12 years. Cytological examination of gingival cytograms was performed in all examined children. Results Results: Based on the analysis of the quantitative content of epithelial cells in children with normal body weight, their ratio was established, which is determined by the percentage of 0: 6: 94 (parabasal, intermediate, superficial). The obtained data completely coincide with the percentage of the differentiated ratio of epitheliocytes of multilayered squamous epithelium in children with normal body weight with inflammation and without it in the periodontal tissues. Our cytological examinations of gingival scrape smears in overweight children in contrast to the results of the study of epithelial scrape smears in children with normal body weight have some differences. Thus, in the process of calculation, the degree of differentiation of various epitheliocytes determines their percentage as follows - 3: 7: 90 (parabasal, intermediate, superficial) for children with gingivitis, and 2: 5: 93 (parabasal, intermediate, superficial) for children without inflammation in the periodontal tissues. Conclusion Conclusions: The obtained results allowed us to conclude that in overweight children, in contrast to children with normal body weight, the number of parabasal cells decreases, and the number of superficial and intermediate cells increases.

Aims Patients with chronic kidney disease (CKD) are also susceptible to periodontitis. The causal link between periodontitis and CKD may be mediated via systemic inflammation/oxidative stress. Using structural equation modelling (SEM), this cross‐sectional study aimed to explore the causal relationship between periodontal inflammation (PI) and renal function. Materials and methods Baseline data on 770 patients with stage 3–5 (pre‐dialysis) CKD from an ongoing cohort study were used. Detailed, bioclinical data on PI and renal function, as well as potential confounders and mediators of the relationship between the two, were collected. SEMs of increasing complexity were created to test the causal assumption that PI affects renal function and vice versa. Results Structural equation modelling confirmed the assumption that PI and renal function are causally linked, mediated by systemic oxidative stress. The magnitude of this effect was such that a 10% increase in PI resulted in a 3.0% decrease in renal function and a 10% decrease in renal function resulted in a 25% increase in PI. Conclusions Periodontal inflammation represents an occult source of oxidative stress in patients with CKD. Further clinical studies are needed to confirm whether periodontal therapy, as a non‐pharmacological approach to reducing systemic inflammatory/oxidative stress burden, can improve outcomes in CKD.