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INTRODUCTION: Chronic liver disease is an important complication observed in haemophilia patients due to viral infections transmitted during treatment with factor concentrates. Our objective was to examine synthetic liver function parameters [fibrinogen, factor V (FV), albumin and Glu-plasminogen] in haemophilia B (HB) patients. METHODS: The study group consisted of 41 HB patients, aged 2-84 years, divided into two groups according to age: HB1≤18 years (N=15) and HB2>18 years (N=26), and 41 healthy male controls (HMC) aged 6-76 years. The Clauss method was used for the determination of fibrinogen concentrations (Dade Thrombin, Sysmex CS5100, Siemens) whereas FV activities were measured by the one-stage clotting assay (Atellica COAG360, Siemens). Albumin concentrations were measured using the bromocresol green colorimetric method (Cobas c501, Roche Diagnostics), whereas Glu-plasminogen was assayed using the ELISA method (Technoclone). The MedCalc® Software was used for statistical analysis. RESULTS: Higher median fibrinogen concentration (3.1 g/L) was encountered in HB patients (range: 2.7-3.6 g/L), compared to the HMC group (median 2.5 g/L ; range: 2.3-2.8 g/L), resulting in a statistically significant difference (P

BACKGROUND: With modern coagulation analyzers, single activated partial thromboplastin time (aPTT) result is not just a number but a collection of photo-optical data in the form of a reaction curve. AIM: To investigate if aPTT reaction curve provides important additional information and whether it allows to predict the degree of FVIII/FIX deficiency from reaction curve in hemophilia patients. METHODS: The study examined plasma samples from 25 healthy, 89 hemophilia A and 17 hemophilia B subjects. aPTT (Actin FS, Atellica COAG360, Siemens, Germany) was determined with two evaluation modes: fixed absorbance (onset of coagulation, FA) and drifting baseline (DB) proposed by manufacturer. Moreover, aPTT at 50% coagulation (PI) was manually determined. We calculated DELTA-1 (aPTT-DB minus aPTT-FA) and DELTA-2 (aPTT-PI minus aPTT-DB). FVIII and FIX activities were measured by one-stage clotting assay and inhibitor using Nijmegen-Bethesda assay. Hemophilia A patients were further divided into HA1 (FVIII5-40IU/dL, N=47) groups, hemophilia B into HB1 (FIX= 1.30 s allowed distinguishing between healthy and hemophilia subjects (sensitivity:100%, specificity:100%) and >3.64 s between patients with and without inhibitors (sensitivity:79.3%, specificity:53.2%). DELTA-2 values >5.90 s and >13.70 s allowed distinguishing between healthy and hemophilia subjects (sensitivity:93.4%, specificity:100%) and between patients with and without inhibitors (sensitivity:75.9%, specificity:74.0%). CONCLUSION: aPTT reaction curve provides important additional information in hemophilia patients. Parameters DELTA-1 and DELTA-2 can be reliable used to distinguish healthy from hemophilia subjects, as well as hemophilia patients with and without inhibitors.

U završnom radu se obrađuje tema Odnos između društveno odgovornog poslovanja i financijskih rezultata na primjeru organizacije dm drogerie markt. Društveno odgovorno poslovanje je određeni koncept poslovanja u kojem tvrtka odlučuje na dobrovoljnoj osnovi doprinijeti boljem društvu i čišćem okolišu, naravno u interakciji s ostalim sudionicima. U radu se pojašnjava dm drogerie markt kao primjer tvrtke koja je izuzetno angažirana u društveno odgovornom poslovanju. Tvrtka potiče brojne akcije u kojima donira sredstva za javnu namjenu. Dobitnik je nekoliko godina zaredom nagrade Indeks za društveno odgovorno poslovanje, kao i nagrade za najboljeg poslodavca u Republici Hrvatskoj. Prikazano je financijsko stanje tvrtke te se istražuje veza između društveno odgovornog poslovanja i uspješnosti tvrtke., In the final paper, the topic is the relationship between socially responsible business and financial results on the example of the dm drogerie markt Socially responsible business is a defined business concept in which a company decides voluntarily to contribute to a better society and a cleaner environment, of course, interacting with other participants. This paper clarifies the dm drogerie markt as an example of a company that is highly engaged in socially responsible business. The company promotes numerous actions in which it grants funds for public use. The winner is several years in a row with the Corporate Social Responsibility Award, as well as the Best Employer Award in the Republic of Croatia. It will show the company's financial status and try to investigate whether socially responsible business has an impact on the company's performance.

Background/Aims Traditionally used laboratory methods, i.e. one-stage or chromogenic assays for FVIII activity determination do not always and accurately reflect bleeding severity in haemophilia A (HA) patients. As global haemostasis assays provide overall haemostatic status estimation, we investigated the ability of three global assays for identifying bleeding phenotype both in severe and non-severe HA patients, as well as their usefulness in laboratory management of HA patients with discrepant bleeding phenotype. Materials and Methods Overall haemostasis potential (OHP), aPTT-clot waveform analysis (aPTT-CWA), endogenous thrombin potential (ETP) and FVIII activity were measured in 30 severe and 32 non- severe HA patients and 27 male controls. For classification of HA patients regarding bleeding phenotype, we used a scoring method that included three clinical parameters: age at first joint bleed, number of target joints and number of joint/muscle bleeds per year. Bleeding scores ≤4 and ≥5 suggested mild and severe beeding phenotype, respectively. Results All global assays correlated significantly with FVIII activity (P

Background: Specific shapes and slopes of the aPTT reaction curve are characteristic for patients with congenital or acquired FVIII/FIX deficiency. Questionable aPTT results are flagged by the analyzer and should be visually inspected. Aims: To investigate the possibility to detect the presence of FVIII/FIX inhibitors by analyzing flagged aPTT results after visual inspection of reaction curves and manual measurement of the reaction curve angle (RCA). Materials and Methods: The study included 131 patient plasma samples with request for the determination of FVIII/FIX inhibitors. aPTT was performed using Actin FS on Atellica COAG 360 (Siemens, Germany) at 405 nm with manufacturer’s proposed evaluation method DRIFTING BASELINE (DB) and checking method ANGLE DB that flagged aPTT results with RCA

Hemophilia A has been considered as a bleeding disorder that is not only a consequence of the defect in the coagulation system, but also of the impaired down-regulation of the fibrinolytic system. To examine correlation between activities and/or concentrations of most important fibrinolytic parameters with overall hemostasis potential (OHP) and fibrin clot permeability (FCP) in hemophilia A patients. Activities of FXIII, plasminogen, plasmin inhibitor, plasminogen activator inhibitor–1 (PAI-1) (Siemens Healthcare Diagnostics, Germany) and thrombin-activatable fibrinolysis inhibitor (TAFI) (Diagnostica Stago, France), as well as activated/inactivated TAFI antigen (TAFIa/ai) (Diagnostica Stago) and prothrombin fragment F1+2 concentrations (PF1+2) (Siemens) were tested in plasma samples of 63 hemophilia A patients (30 severe, 33 non-severe). OHP method, based on repeated spectrophotometric registration of fibrin-aggregation in plasma, after addition of small amounts of exogenous thrombin, tissue plasminogen activator and calcium, gave beside OHP parameter (area under the fibrin aggregation curve) 3 supplementary parameters: overall coagulation potential (OCP), overall fibrinolytic potential (OFP) and clot lysis time (CLT). Permeability coefficients (Ks), providing information on fibrin network porosity, were obtained by FCP method, flow measurement technique along with visualisation by electron microscopy scanning. Among fibrinolytic and OHP parameters significant but weak correlation (P< ; 0.05) was found only for OCP with TAFI and PF1+2 (r=0.252 and 0.266, respectively). Regarding FCP, significant correlation of Ks was found with FXIII, plasmin inhibitor and PF1+2 (r=-0.466, - 0.432 and -0.599, respectively). There was no correlation between Ks and OHP parameters. Obtained correlation between fibrinolytic parameters and parameters that assess coagulation activity and fibrin network porosity confirmed the association between these two processes in patients with hemophilia A.

Measurement of FVIII activity allows diagnosis of hemophilia A and categorization of disease severity, but has poor correlation with clinical phenotype. New laboratory methods that assess global hemostasis have been developed, with intention to better diagnose and monitor hemophilia A patients. To assess overall coagulation and fibrinolytic activity in hemophilia A patients using non- standard laboratory methods: overall hemostasis potential (OHP), aPTT-waveform analysis (aPTT- WA) and endogenous thrombin potential (ETP). Total of 63 hemophilia A patients (30 severe and 33 non-severe) and 27 healthy male subjects as control group were tested. OHP method, based on repeated spectrophotometric registration of the fibrin-aggregation in plasma, after addition of small amounts of exogenous thrombin, tissue-type plasminogen activator and calcium, provides besides OHP parameter (area under the fibrin aggregation curve) supplemetary parameters: overall coagulation potential (OCP), overall fibrinolytic potential (OFP) and clot lysis time (CLT). In-house aPTT- WA was performed on BCS with Actin FS (Siemens Healthcare Diagnostics, Germany), obtaining 3 quantitative waveform parameters from 2 different evaluation modes, drifting baseline (DB) and point of inflexion (PI): DELTA (aPTT- PI minus aPTT-DB), RATIO1 (aPTT- PI/aPTT-DB) and RATIO2 (DELTA/aPTT-DB). ETP method, setting C was performed on BCS-XP (Siemens), giving 4 parameters: area under the thrombin generation curve (AUC), peak thrombin concentration (Cmax), time to peak thrombin concentration (t- max) and time to signal beginning (t-lag). Obtained results revealed statistically significant difference (P< ; 0.05) for all parameters between analyzed groups, except for CLT between severe and non-severe group. Global assays can serve as a useful laboratory tool for assessing overall coagulation and fibrinolysis activity, providing at the same time additional information about hemophilia A patients.

Uvod: Kondenzat izdaha je uzorak koji se u novije vrijeme može uzorkovati na standardiziran i jednostavan način, a velik se napor ulaže u validaciju metoda za određivanje pojedinih analita (pH, interleukini, prostaglandini, urati, itd. ) koji nam mogu pomoći u postavljanju dijagnoze mnogih bolesti dišnog sustava. Cilj: Ciljevi istraživanja bili su ispitati točnost, preciznost i linearnost određivanja kalcija, magnezija i urata u uzorcima standardnih pripravaka i preciznost u pool- uzorcima kondenzata izdaha. Materijali i metode: Koncentracije kalcija, magnezija i urata određene su u uzorcima standardnih pripravaka proizvođača reagensa (Beckman Coulter) i pool-u od 8 uzoraka KI-a koji je alikvotiran u 20 uzoraka. Kao mjere preciznosti i točnosti izračunati su koeficijent varijacije (CV) i odstupanje od ciljnih vrijednosti (bias). Rezultati:Najmanje koncentracije kalcija (koncentracija od 32 μmol/L ; bias = 56 %) i magnezija (koncentracija 17 μmol/L, bias =59 % i koncentracija 37 μmol/L, bias = 16 %) nije moguće odrediti dovoljno točno. Preciznost je za oba analita u otopinama standarda bila bolja, iako je za koncentraciju magnezija od 17 μmol/L bila nezadovoljavajuća (CV = 33 % ). Veće koncentracije kalcija (121 – 1205 μmol/L) i magnezija (73 – 365 μmol/L) kao i koncentraciju urata (6 – 157 μmol/L ) moguće je točno i precizno određivati. U kondenzatu izdaha najpreciznije se određivala koncentracija urata (159 μmol/ ; CV = 0, 6 %), a potom koncentracija kalcija (42 μmol/L ; CV = 10 %). Koncentracija magnezija od 24 μmol/L u EBC- u određena je s CV od 21 %. Zaključak:Određivanje urata, te većih koncentracija kalcija i magnezija u otopinama standarda točno je i precizno. U kondenzatu izdaha pouzdano i točno mogu se odrediti koncentracije urata i kalcija. Za pouzdano određivanje vrlo malih mikromolarnih koncentracija kalcija i magnezija potrebne su osjetljivije metode.

Questa tesi di laurea si propone di esaminare il tema del disagio esistenziale nelle opere di Paolo Giordano, uno dei migliori giovani scrittori italiani, il cui romanzo La solitudine dei numeri primi ottiene grande successo. Oltre a questo romanzo, viene analizzata un'altra opera di Giordano, Il corpo umano. Entrambi romanzi trattano lo stesso argomento - l'impossibilità dell'individuo di trovare il suo luogo nella società.

This paper deals with the semantic adaptation of anglicisms in Italian newspapers. All anglicisms from the corpus are collected from two Italian daily papers during the period of three months, and they belong to different domains (economy, business, politics, sport, entertainment, ifestyle, science and technology). The classification is based on the approach by Rudolf. The information from the research is compared to the ones collected by M. Komu (1998).

U članku se na dva pogodno odabrana primjera iz gospodarske prakse ilustrira primjena računalnoga programa Maxima na rješavanje nekih problema iz primjene diferencijalnoga računa više varijabli u ekonomiji (optimizacija funkcije dobiti, izračun i interpretacija koeficijenata parcijalne elastičnosti). Odabrani primjeri predstavljaju uzorak zadataka kakve studenti specijalističkoga studija trgovinskoga poslovanja Veleučilišta u Požegi rješavaju u okviru predmeta Kvantitativne metode u trgovini na završnoj godini toga studija. Primjeri su riješeni metodički u skladu s postavljenim ciljevima i ishodima navedenoga predmeta.