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Additional file 1. Supplementary materials : Figure S1 Antibody-labeled cells were shown to have high expression of CD105(95.4%), CD73(99.2%), and CD90(96%) and low or no expression of CD45(0.14%) and CD34(0.16%) by flow cytometry. These results met the criteria for identifying mesenchymal stem cells by surface marker expression. Table S1 The tumorigenicity test results of NOD/SCID mice administered human umbilical cord mesenchymal stem cell injection (body weight (g, mean��SD)). Table S2 The tumorigenicity test results of NOD/SCID mice administered human umbilical cord mesenchymal stem cell injection(nodule volume (mm3, mean��SD)). Table S3 The effect of human umbilical cord mesenchymal stem cell injection on tumor growth in HeLa cell-derived tumor-bearing NOD/SCID mice (weight (g, mean��SD)). Table S4 The effect of human umbilical cord mesenchymal stem cell injection on tumor growth in HeLa cell-derived tumor-bearing NOD/SCID mice (tumor volume (mm3, mean��SD)). Table S5 The effect of human umbilical cord mesenchymal stem cell injection on tumor growth in HeLa cell-derived tumor-bearing NOD/SCID mice (RTV). Table S6 The effect of human umbilical cord mesenchymal stem cell injection on tumor growth in HeLa cell-derived tumor-bearing NOD/SCID mice (T/C%). Table S7 The effect of human umbilical cord mesenchymal stem cell injection on tumor growth in HeLa cell-derived tumor-bearing NOD/SCID mice (tumor weight (g, mean��SD) and tumor weight inhibition rate (%)). Table S8 The effect of human umbilical cord mesenchymal stem cell injection on tumor growth in Raji cell-derived tumor-bearing NOD/SCID mice (weight (g, mean��SD)). Table S9: Effects of human umbilical cord mesenchymal stem cell injection on tumor growth in Raji cell-derived tumor-bearing NOD/SCID mice (tumor volume (mm3, mean��SD)). Table S10 The effect of human umbilical cord mesenchymal stem cell injection on tumor growth in Raji cell-derived tumor-bearing NOD/SCID mice (RTV). Table S11 The effect of human umbilical cord mesenchymal stem cell injection on tumor growth in Raji cell-derived tumor-bearing NOD/SCID mice (T/C%). Table S12 The effect of human umbilical cord mesenchymal stem cell injection on tumor growth in Raji cell-derived tumor-bearing NOD/SCID mice (tumor weight (g, mean��SD) and tumor weight inhibition rate (%)).

Presents the methods for quantitative PCR (three steps in total). (DOCX 17 kb)