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Climate change affects timing of reproduction in many bird species, but few studies have investigated its influence on annual reproductive output. Here, we assess changes in the annual production of young by female breeders in 201 populations of 104 bird species (N = 745,962 clutches) covering all continents between 1970 and 2019. Overall, average offspring production has declined in recent decades, but considerable differences were found among species and populations. A total of 56.7% of populations showed a declining trend in offspring production (significant in 17.4%), whereas 43.3% exhibited an increase (significant in 10.4%). The results show that climatic changes affect offspring production through compounded effects on ecological and life history traits of species. Migratory and larger-bodied species experienced reduced offspring production with increasing temperatures during the chick-rearing period, whereas smaller-bodied, sedentary species tended to produce more offspring. Likewise, multi-brooded species showed increased breeding success with increasing temperatures, whereas rising temperatures were unrelated to reproductive success in single-brooded species. Our study suggests that rapid declines in size of bird populations reported by many studies from different parts of the world are driven only to a small degree by changes in the production of young., This meta-analysis was financed by the grant of the Polish National Science Centre (Narodowe Centrum Nauki) (no. 2017/27/B/NZ8/00465) awarded to Lucyna Hałupka.

1.Evidence that exposure to environmental pollutants can alter the gut microbiota composition of wildlife includes studies of rodents exposed to radionuclides. 2.Antwis et al. (2021) used amplicon sequencing to characterise the gut microbiota of four species of rodent (Myodes glareolus, Apodemus agrarius, A. flavicollis and A. sylvaticus) inhabiting the Chernobyl Exclusion Zone (CEZ) to examine possible changes in gut bacteria (microbiota) and gut fungi (mycobiota) associated with exposure to radionuclides and whether the sample type (from caecum or faeces) affected the analysis. 3.The conclusions derived from the analyses of gut mycobiota are based on data that represent a mixture of ingested fungi (e.g. edible macrofungi, polypores, lichens and ectomycorrhizae) and gut mycobiota (e.g. microfungi and yeasts), which mask the patterns of inter- and intraspecific variation in the authentic gut mycobiota. 4.Implying that ‘faecal samples are not an accurate indicator of gut composition’ creates an unnecessary controversy about faecal sampling because the comparison of samples from the caecum and faeces confounds many other possible drivers (including different animals from different locations, sampled in different years) of variation in gut microbiota. 5.It is relevant also that Antwis et al.'s (2021) data lack statistical power to detect an effect of exposure to radionuclides on the gut microbiota because (1) all of their samples of Apodemus mice had experienced a medium or high total absorbed dose rate and (2) they did not collect samples of bank voles (M. glareolus) from replicate contaminated and uncontaminated locations. 6.Discussion of Antwis et al.'s (2021) analysis, especially the claims presented in the Abstract, is important to prevent controversy about the outcome of research on the biological impacts of wildlife inhabiting the CEZ. peerReviewed

Comprehending symbiont abundance among host species is a major ecological endeavour, and the metabolic theory of ecology has been proposed to understand what constraints symbiont populations. We parameterized metabolic theory equations to predict how bird species’ body size and the body size of their feather mites relate to mite abundance according to four potential energy (microbial abundance, uropygial gland size) and space constraints (wing area, number of feather barbs). Predictions were compared with the empirical scaling of feather mite abundance from 26,604 birds of 106 passerine species, using phylogenetic modelling and quantile regression. Feather mite populations were strongly constrained by host space (number of feather barbs) and not energy. Moreover, feather mite species’ body size was unrelated to their abundance or to the body size of their host species. We discuss the implications of our results for our understanding of the bird-feather mite system and for symbiont abundance in general.

Aims: The novelty of the COVID-19 pandemic and fast-developed vaccines may increase concerns about the safety and effectiveness of the COVID-19 vaccine and thereby influence vaccine hesitancy. The aims of this study were to examine (a) the main reasons for COVID-19 vaccine hesitancy and (b) factors associated with COVID-19 vaccine hesitancy. Methods: A nationwide survey was conducted in the autumn of 2021 when most Danes had received at least one dose of a COVID-19 vaccine. The sample ( N=13,570) was based on a random selection of individuals (⩾15 years), and data were collected via self-administered mailed questionnaires (digital and physical). The participants were asked whether they had been vaccinated against COVID-19, and if they had not, they were asked about their reasons for vaccine hesitancy. Logistic regression models were used to determine factors associated with vaccine hesitancy. Results: In all, 5,384 (39.7%) individuals completed the questionnaire. The main reasons for vaccine hesitancy were worries about adverse effects (57.6%) and the belief that natural immunity is better than vaccination (43.8%). Factors associated with vaccine hesitancy included female sex, younger age, being divorced, a country of origin other than Denmark, lower educational level, being unemployed, previously infected with COVID-19 and being worried to a greater extent about oneself and others getting infected. Mental health indicators were not associated with vaccine hesitancy. Conclusions: COVID-19 vaccine hesitancy is likely to pose a challenge for population immunity. To reduce vaccine hesitancy and increase vaccination uptake, future vaccination programmes should focus on specific socio-demographic subgroups identified in this study.

Background COVID-19 caused economic insecurity for businesses and their employees. Understanding effects of changes in labor force participation on depression risk during economic recession is fundamental for early diagnosis. The study evaluates if changes in labor force participation are associated with depression risk during COVID-19 in Denmark. Methods A register-based longitudinal study of Danes aged 25–67 years without depression 2 years prior to baseline defined as February 2020. An eight-level categorical variable on stable or changing labor force participation was defined from monthly employment percentage gradients in the Danish Register-based Evaluation and Marginalization Database from February 2020. The cohort was followed until 31 December 2020 for depressions overall and mild-, moderate- and severe depression. Sex-stratified cox regression models with hazard ratios (HR) and 95% confidence intervals (95% CI) were performed accounting for important confounders. Results In total, 1 619 240 (50.3%) men of mean age 45.6 years and 1 598 587 (49.7%) women of mean age 45.9 years were included. Becoming unemployed implied an increased HR of depression in men (HR 2.02; 95% CI 1.94–2.10) and women (2.19; 2.12–2.26) compared to a steady-state full-time employment. Being outside the labor force or employed part-time implied an elevated HR in men (3.02; 2.82–3.23 and 2.41; 2.35–2.48) and women (3.13; 2.30–3.31 and 2.30; 2.26–2.35), respectively, compared to a steady-state full-time employment. Conclusions Changes in labor force participation were associated with higher risk of depression relative to a steady-state full-time employment particularly among individuals with low labor force participation during COVID-19.

Summary paragraphVacuolar-type adenosine triphosphatases (V-ATPases)1–3 are electrogenic rotary mechanoenzymes structurally related to F-type ATP synthases4,5. They hydrolyze ATP to establish electrochemical proton gradients for a plethora of cellular processes1,3. In neurons, the loading of all neurotransmitters into synaptic vesicles is energized by ~1 V-ATPase molecule per synaptic vesicle6,7. To shed light into this bona fide single-molecule biological process, we investigated electrogenic proton pumping by single mammalian-brain V-ATPases, using individual synaptic vesicles fused with immobilized liposomes. We show V-ATPases do not pump continuously in time, as hypothesized by observing the rotation of bacterial homologs8 and assuming strict ATP/proton coupling. Instead, they stochastically switch between three novel ultra-long-lived proton-pumping, inactive, and proton-leaky modes. Upending conventional wisdom, direct observation of pumping revealed that physiologically relevant concentrations of ATP do not regulate the intrinsic pumping rate. Instead, ATP regulates V-ATPase activity via the switching probability of the proton-pumping mode. In contrast, electrochemical proton gradients regulate the pumping rate and the switching of the pumping and inactive modes. This work reveals and emphasises the mechanistic and biological importance of mode-switching in protein regulation.

We report on the stellar content, half-light radii and star formation rates of a sample of 10 known high-redshift ($z\gtrsim 2$) galaxies selected on strong neutral hydrogen (HI) absorption (log(N(HI)/cm$^{-2})>19$) toward background quasars. We use observations from the {\it Hubble Space Telescope} (HST) Wide Field Camera 3 in three broad-band filters to study the spectral energy distribution(SED) of the galaxies. Using careful quasar point spread function subtraction, we study their galactic environments, and perform the first systematic morphological characterisation of such absorption-selected galaxies at high redshifts. Our analysis reveals complex, irregular hosts with multiple star-forming clumps. At a spatial sampling of 0.067 arcsec per pixel (corresponding to 0.55 kpc at the median redshift of our sample), 40% of our sample requires multiple S��rsic components for an accurate modelling of the observed light distributions. Placed on the mass-size relation and the `main sequence' of star-forming galaxies, we find that absorption-selected galaxies at high redshift extend known relations determined from deep luminosity-selected surveys to an order of magnitude lower stellar mass, with objects primarily composed of star-forming, late-type galaxies. We measure half-light radii in the range $r_{1/2} \sim$ 0.4 to 2.6 kpc based on the reddest band (F160W) to trace the oldest stellar populations, and stellar masses in the range $\log (\mathrm{M}_{\star}/\mathrm{M}_{\odot}) \sim$ 8 to 10 derived from fits to the broad-band SED. Spectroscopic and SED-based star formation rates are broadly consistent, and lie in the range log(SFR/M$_{\odot} $yr$^{-1}$) $\sim$0.0 to 1.7., 17 pages, Accepted for publication in MNRAS. This revision has minor text changes

Among avian species, the differential cost entailed by either sex in competition for mates has been regarded as the main evolutionary influence on sex differences in mortality rates. However, empirical evidence suggests that sex-biased adult mortality is mainly related to differential energy investment in gamete production, with a greater annual mass devoted to egg production leading to higher female mortality. We explicitly tested the generality of this pattern in a comparative framework. Annual egg production can be relatively large in some species (up to 200% of female body mass) and annual mortality is generally biased toward females. We showed that greater annual egg productivity resulted in higher mortality rates of females relative to males. Mating system was secondarily important, with species in which males were more involved in mating competition having more equal mortality rates between the sexes. However, both traits explained only a limited fraction of the interspecific variation in female-biased mortality. Other traits, such as sexual size dimorphism and parental care, had much weaker influences on female-biased mortality. Our results suggest that both annual mass devoted to gamete production by females and mating system contribute to the evolution of the fundamental life-history trade-off between reproduction and survival in avian taxa.

River estuaries are characterized by mixing processes between freshwater discharge and marine water masses. Since the first are depleted in heavier stable isotopes compared with the marine realm, estuaries often show a linear correlation between salinity and water stable isotopes (δ18O and δ2H values). In this study, we evaluated spatial and seasonal isotope dynamics along three estuarine lagoon transects, located at the northern German Baltic Sea coast. The data show strong seasonality of isotope values, even at locations located furthest from the river mouths. They further reveal a positive and linear salinity‐isotope correlation in spring, but ‐in two of the three studied transects‐ hyperbolic and partially reverse correlations in summers. We conclude that additional hydrological processes partially overprint the two‐phase mixing correlation during summers: aside from the isotope seasonality of the riverine inflows, the shallow inner lagoons in the studied estuaries are influenced by evaporation processes. In contrast the estuarine outflow regions are under impact of significant salinity and isotope fluctuations of the Baltic Sea. Deciphering those processes is crucial for the understanding of water isotope and salinity dynamics. This is also of relevance in context of ecological studies, for example, when interpreting oxygen and hydrogen isotope data in aquatic organisms that depend on ambient estuarine waters., Spatial and seasonal water isotope dynamics were evaluated along three estuarine lagoon transects at the German Baltic Sea coast. Data reveal a positive and linear salinity‐isotope correlation in spring, but partially reverse correlations in summers. The results show that evaporation processes in the shallow inner lagoons and varying Baltic Sea influence in the outer estuary regions are able to overprint the two‐phase mixing correlation between riverine and marine water masses., Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659, https://doi.org/10.1594/PANGAEA.937990

Migratory animals experience very different environmental conditions at different times of the year, i.e., at the breeding grounds, during migration, and in winter. The long-tailed duck Clangula hyemalis breeds in the Arctic regions of the northern hemisphere and migrates to temperate climate zones, where it winters in marine environments. The breeding success of the long-tailed duck is affected by the abundances of predators (mainly Arctic fox Alopex lagopus) and their main prey species, lemmings Lemmus sibiricus and Dicrostonyx torquatus, whose population fluctuation is subject to climate change. In the winter quarters, long-tailed ducks mainly eat the blue mussel Mytilus edulis. We examined how North-west Siberian lemming dynamics affect long-tailed duck breeding success via predation pressure and how nutrient availability in the Baltic Sea influences long-tailed duck population size via mussel biomass and quality. The long-tailed duck population dynamics was predator-driven on the breeding grounds and resource-driven on the wintering grounds. Nutrients from fertilizer runoff from farmland stimulate mussel stocks and quality, supporting high long-tailed duck population sizes. The applied hierarchical analysis combining several trophic levels can be used for evaluating large-scale environmental factors that affect the population dynamics and abundance of migrants from one environment to another.

Sperm quantity and quality are key features explaining intra- and interspecific variation in male reproductive success. Spermatogenesis is sensitive to ionizing radiation and laboratory studies investigating acute effects of ionizing radiation have indeed found negative effects of radiation on sperm quantity and quality. In nature, levels of natural background radiation vary dramatically, and chronic effects of low-level background radiation exposure on spermatogenesis are poorly understood. The Chernobyl region offers a unique research opportunity for investigating effects of chronic low-level ionizing radiation on reproductive properties of wild organisms. We captured male bank voles (Myodes glareolus) from 24 locations in the Chernobyl exclusion zone in 2011 and 2015 and collected information on sperm morphology and kinetics. The dataset is limited in size and there overall was a relatively weak correlation between background radiation and sperm quality. Still, some correlations are worth discussing. First, mid-piece segments of spermatozoa tended to be smaller in bank vole males from areas with elevated background radiation levels. Second, we demonstrated a significant positive relationship between background radiation dose rates and the proportion of static spermatozoa among males within and among study locations after 10 as well as 60 min of incubation. Our results provide novel evidence of damaging effects of low dose ionizing radiation on sperm performance in wild rodent populations, and highlight that this topic requires further study across the natural gradients of background radiation that exist in nature.

Full list of authors: Saccardi, A.; Vergani, S. D.; De Cia, A.; D'Elia, V.; Heintz, K. E.; Izzo, L.; Palmerio, J. T.; Petitjean, P.; Rossi, A.; Postigo, A. de Ugarte; Christensen, L.; Konstantopoulou, C.; Levan, A. J.; Malesani, D. B.; Moller, P.; Ramburuth-Hurt, T.; Salvaterra, R.; Tanvir, N. R.; Thone, C. C.; Vejlgaard, S.; Fynbo, J. P. U.; Kann, D. A.; Schady, P.; Watson, D. J.; Wiersema, K.; Campana, S.; Covino, S.; De Pasquale, M.; Fausey, H.; Hartmann, D. H.; van der Horst, A. J.; Jakobsson, P.; Palazzi, E.; Pugliese, G.; Savaglio, S.; Starling, R. L. C.; Stratta, G.; Zafar, T.-- This is an Open Access article, published by EDP Sciences, under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited., The study of the properties of galaxies in the first billion years after the Big Bang is one of the major topics of current astrophysics. Optical and near-infrared spectroscopy of the afterglows of long gamma-ray bursts (GRBs) provides a powerful diagnostic tool to probe the interstellar medium (ISM) of their host galaxies and foreground absorbers, even up to the highest redshifts. We analyze the VLT/X-shooter afterglow spectrum of GRB 210905A, triggered by the Neil Gehrels Swift Observatory, and detect neutral hydrogen, low-ionization, high-ionization, and fine-structure absorption lines from a complex system at z = 6.3118, which we associate with the GRB host galaxy. We use them to study the ISM properties of the host system, revealing the metallicity, kinematics, and chemical abundance pattern of its gas along the GRB line of sight. We also detect absorption lines from at least two foreground absorbers at z = 5.7390 and z = 2.8296. The total metallicity of the z ∼ 6.3 system is [M/H]tot = −1.72 ± 0.13, after correcting for dust depletion and taking α-element enhancement into account, as suggested by our analysis. This is consistent with the values found for the other two GRBs at z ∼ 6 with spectroscopic data showing metal absorption lines (GRB 050904 and GRB 130606A), and it is at the higher end of the metallicity distribution of quasar damped Lyman-α systems (QSO-DLAs) extrapolated to such a high redshift. In addition, we determine the overall amount of dust and dust-to-metal mass ratio (DTM) ([Zn/Fe]fit = 0.33 ± 0.09 and DTM = 0.18 ± 0.03). We find indications of nucleosynthesis due to massive stars and, for some of the components of the gas clouds, we find evidence of peculiar nucleosynthesis, with an overabundance of aluminum (as also found for GRB 130606A). From the analysis of fine-structure lines, we determine distances of several kiloparsecs for the low-ionization gas clouds closest to the GRB. Those are farther distances than usually found for GRB host absorption systems, possibly due to the very high number of ionizing photons produced by the GRB that could ionize the line of sight up to several hundreds of parsecs. Using the HST/F140W image of the GRB field, we show the GRB host galaxy (with a possible afterglow contamination) as well as multiple objects within 2″ from the GRB position. We discuss the galaxy structure and kinematics that could explain our observations, also taking into account a tentative detection of Lyman-α emission at z = 6.3449 (∼1200 km s−1 from the GRB redshift in velocity space), and the observational properties of Lyman-α emitters at very high redshift. This study shows the amazing potential of GRBs to access detailed information on the properties (metal enrichment, gas kinematic, dust content, nucleosynthesis...) of very high-redshift galaxies, independently of the galaxy luminosity. Deep spectroscopic observations with VLT/MUSE and JWST will offer the unique possibility of combining the information presented in this paper with the properties of the ionized gas, with the goal of better understanding how galaxies in the reionization era form and evolve. © The Authors 2023., This work was supported by CNES. A.S. and S.D.V. acknowledge support from DIM-ACAV+. D.A.K. acknowledges support from Spanish National Research Project RTI2018-098104-J-I00 (GRBPhot). A.J.L. and D.B.M. are supported by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 725246). The Cosmic Dawn Center is funded by the Danish National Research Foundation under grant No. 140. NRT is supported by STFC consolidated grant ST/W000857/1. A.R., E.P., G.S. and S.S. acknowledge support from PRIN-MIUR 2017 (grant 20179ZF5KS). G.S. acknowledges the support by the State of Hesse within the Research Cluster ELEMENTS (Project ID 500/10.006)., With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2021-001131-S).

Full list of authors: Melandri, A.; Izzo, L. ; Pian, E.; Malesani, D. B.; Della Valle, M.; Rossi, A.; D'Avanzo, P.; Guetta, D.; Mazzali, P. A.; Benetti, S.; Masetti, N.; Palazzi, E.; Savaglio, S.; Amati, L.; Antonelli, L. A.; Ashall, C.; Bernardini, M. G.; Campana, S.; Carini, R.; Covino, S.; D'Elia, V.; de Ugarte Postigo, A.; De Pasquale, M.; Filippenko, A. V.; Fruchter, A. S.; Fynbo, J. P. U.; Giunta, A.; Hartmann, D. H.; Jakobsson, P.; Japelj, J.; Jonker, P. G.; Kann, D. A.; Lamb, G. P.; Levan, A. J.; Martin-Carrillo, A.; Møller, P.; Piranomonte, S.; Pugliese, G.; Salvaterra, R.; Schulze, S.; Starling, R. L. C.; Stella, L.; Tagliaferri, G.; Tanvir, N.; Watson, D., We observed GRB 190114C (redshift z = 0.4245), the first gamma-ray burst (GRB) ever detected at TeV energies, at optical and near-infrared wavelengths with several ground-based telescopes and the Hubble Space Telescope, with the primary goal of studying its underlying supernova, SN 2019jrj. The monitoring spanned the time interval between 1.3 and 370 days after the burst, in the observer frame. We find that the afterglow emission can be modelled with a forward shock propagating in a uniform medium modified by time-variable extinction along the line of sight. A jet break could be present after 7 rest-frame days, and accordingly the maximum luminosity of the underlying supernova (SN) ranges between that of stripped-envelope core-collapse SNe of intermediate luminosity and that of the luminous GRB-associated SN 2013dx. The observed spectral absorption lines of SN 2019jrj are not as broad as in classical GRB SNe and are instead more similar to those of less-luminous core-collapse SNe. Taking the broad-lined stripped-envelope core-collapse SN 2004aw as an analogue, we tentatively derive the basic physical properties of SN 2019jrj. We discuss the possibility that a fraction of the TeV emission of this source might have had a hadronic origin and estimate the expected high-energy neutrino detection level with IceCube. © ESO 2022., A. M., M. G. B., P. D. A., S. C., and G. T. acknowledge support from ASI grant I/004/11/5. P. D. A., M. D. V., E. Pa., S. Sa., and S. P. acknowledge support from PRIN-MIUR 2017 (grant 20179ZF5KS). L. I. was supported by the VILLUM FONDEN (project numbers 16599 and 25501). D. B. M. acknowledges support from research grant 19054 from VILLUM FONDEN. A. V. F.'s research is supported by the Christopher R. Redlich Fund, the Miller Institute for Basic Research in Science (in which he is a Senior Miller Fellow), and many individual donors. D. A. K. acknowledges support from Spanish National Research Project RTI2018-098104-JI00 (GRBPhot). D. W. is supported by Independent Research Fund Denmark grant DFF-7014-00017. The Cosmic Dawn Center is supported by the Danish National Research Foundation under grant 140. A. R. acknowledges support from the project Supporto Arizona and Italia. The LBT is an international collaboration among institutions in the United States, Italy, and Germany. LBT Corporation partners are The University of Arizona on behalf of the Arizona Board of Regents; Istituto Nazionale di Astrofisica, Italy; LBT Beteiligungsgesellschaft, Germany, representing the Max-Planck Society, The Leibniz Institute for Astrophysics Potsdam, and Heidelberg University; The Ohio State University, representing OSU, University of Notre Dame, University of Minnesota, and University of Virginia. This work made use of data supplied by the UK Swift Science Data Centre at the University of Leicester., With funding from the Spanish government through the Severo Ochoa Centre of Excellence accreditation SEV-2017-0709.

We present a pilot search of CO emission in three H2-absorbing, long-duration gamma-ray burst (GRB) host galaxies at z ∼ 2-3. We used the Atacama Large Millimeter/submillimeter Array (ALMA) to target the CO(3 - 2) emission line and report nondetections for all three hosts. These are used to place limits on the host molecular gas masses, assuming a metallicity-dependent CO-to-H2 conversion factor (αCO). We find, Mmol < 3.5 × 1010M⊙ (GRB 080607), Mmol < 4.7 × 1011M⊙ (GRB 120815A), and Mmol [removed]1 with M∗ < 1010M⊙. To better quantify this we develop a simple approach to estimate the relevant αCO factor based only on the redshift and stellar mass of individual galaxies. The elevated conversion factors will make these galaxies appear CO-'dark' and difficult to detect in emission, as is the case for the majority of GRB hosts. GRB spectroscopy thus offers a complementary approach to identify low-metallicity, star-forming galaxies with abundant molecular gas reservoirs at high redshifts that are otherwise missed by current ALMA surveys. © 2021 The Author(s) Published by Oxford University Press on behalf of Royal Astronomical Society., KEH and PJ acknowledge support by a Project Grant (162948-051) from The Icelandic Research Fund. MN acknowledges support from ERC Advanced grant 740246 (Cosmic Gas). JPUF thanks the Carlsberg Foundation for support. The Cosmic DAWN Center is funded by the DNRF. PN and JKK acknowledge support from the French Agence Nationale de la Recherche under grant ANR 17-CE31-001101/project `HIH2' (PI: Noterdaeme). This paper makes use of the following ALMA data: ADS/JAO.ALMA#2019.1.00407.S. ALMA is a partnership of ESO (representing its member states), NSF (USA) and NINS (Japan), together with NRC (Canada), MOST and ASIAA (Taiwan), and KASI (Republic of Korea), in cooperation with the Republic of Chile. The Joint ALMA Observatory is operated by ESO, AUI/NRAO and NAOJ., With funding from the Spanish government through the Severo Ochoa Centre of Excellence accreditation SEV-2017-0709.

Tamaño cefálico y personalidad del carbonero común, Parus major El comportamiento y la toma de decisiones dependen de las capacidades cognitivas y, en último término, del tamaño y la estructura del encéfalo. Nuestra hipótesis es que la personalidad puede estar relacionada con el tamaño relativo del encéfalo respecto del tamaño corporal y, por consiguiente, que la selección actúa en contra de los individuos que tienen encéfalos pequeños con respecto al tamaño del cuerpo. Analizamos las puntuaciones habituales de personalidad en individuos de carbonero común, Parus major, capturados en el campo, en relación con el volumen cefálico, el sexo, la edad, la fecha de captura y el tamaño corporal. Debido a la fuerte correlación positiva existente entre el volumen cefálico y la masa encefálica, se pudo emplear una técnica no destructiva para estimar el tamaño encefálico a partir del volumen cefálico. La puntuación de la personalidad estaba positivamente correlacionada con el volumen cefálico y fue superior en individuos que se capturaron más entrada la estación. En un análisis del volumen cefálico en relación con el sexo, la edad, la fecha de captura y el tamaño corporal, se observó que la cabeza era mayor en los machos y en los individuos de años anteriores que en las hembras y los jóvenes del año, respectivamente. El volumen cefálico era mayor en los individuos que se capturaron más entrada la estación. Estos resultados coinciden con la predicción de que la personalidad está relacionada con el tamaño encefálico relativo y que la selección actúa sobre la personalidad y el tamaño cefálico relativo, tal como reflejan los cambios producidos en el tiempo y entre clases de edad., Behavior and decision–making depend on cognitive abilities and ultimately brain size and structure. I hypothesized that personality may be related to relative brain size adjusted for body size, and therefore, that selection acts against individuals that have small brains for their body size. I investigated standard personality scores in great tits Parus major from the field in relation to head volume, sex, age, capture date, and body size. Head volume and brain mass were strongly positively correlated, allowing for non–destructive estimation of brain size based on head volume. Personality score was positively correlated with head volume andwas higher in individuals captured later in the season. In an analysis of head volume in relation to sex, age, date of capture and body size, males had larger heads than females and older individuals had larger heads than yearlings. Head volume was larger in individuals captured later during the season. These findings are consistent with the prediction that personality is related to relative brain size and that selection acts on personality and relative head size as reflected by changes over time and between age classes.

International audience; The marks of prehistoric human societies on tropical forests can still be detected today. Levis et al. performed a basin-wide comparison of plant distributions, archaeological sites, and environmental data. Plants domesticated by pre-Columbian peoples are much more likely to be dominant in Amazonian forests than other species. Furthermore, forests close to archaeological sites often have a higher abundance and richness of domesticated species. Thus, modern-day Amazonian tree communities across the basin remain largely structured by historical human use.Science, this issue p. 925The extent to which pre-Columbian societies altered Amazonian landscapes is hotly debated. We performed a basin-wide analysis of pre-Columbian impacts on Amazonian forests by overlaying known archaeological sites in Amazonia with the distributions and abundances of 85 woody species domesticated by pre-Columbian peoples. Domesticated species are five times more likely than nondomesticated species to be hyperdominant. Across the basin, the relative abundance and richness of domesticated species increase in forests on and around archaeological sites. In southwestern and eastern Amazonia, distance to archaeological sites strongly influences the relative abundance and richness of domesticated species. Our analyses indicate that modern tree communities in Amazonia are structured to an important extent by a long history of plant domestication by Amazonian peoples.

In eukaryotes, P-type adenosine triphosphatases (ATPases) generate the plasma membrane potential and drive secondary transport systems; however, despite their importance, their regulation remains poorly understood. We monitored at the single-molecule level the activity of the prototypic proton-pumping P-type ATPase Arabidopsis thaliana isoform 2 (AHA2). Our measurements, combined with a physical nonequilibrium model of vesicle acidification, revealed that pumping is stochastically interrupted by long-lived (~100 seconds) inactive or leaky states. Allosteric regulation by pH gradients modulated the switch between these states but not the pumping or leakage rates. The autoinhibitory regulatory domain of AHA2 reduced the intrinsic pumping rates but increased the dwell time in the active pumping state. We anticipate that similar functional dynamics underlie the operation and regulation of many other active transporters.

Context. Absorbing galaxies are selected via the detection of characteristic absorption lines which their gas-rich media imprint in the spectra of distant light-beacons. The proximity of the typically faint foreground absorbing galaxies to bright background sources makes it challenging to robustly identify these in emission, and hence to characterise their relation to the general galaxy population. Aims. We search for emission to confirm and characterise ten galaxies hosting damped, metal-rich quasar absorbers at redshift z < 1. Methods. We identify the absorbing galaxies by matching spectroscopic absorption -and emission redshifts and from projected separations. Combining emission-line diagnostics with existing absorption spectroscopy and photometry of quasar-fields hosting metal-rich, damped absorbers, we compare our new detections with reference samples and place them on scaling relations. Results. We spectroscopically confirm seven galaxies harbouring damped absorbers (a 70% success-rate). Our results conform to the emerging picture that neutral gas on scales of tens of kpc in galaxies is what causes the characteristic Hi absorption. Our key results are: (I) Absorbing galaxies with $\log _{10} [M_\star ~(M_\odot)] \gtrsim 10$ have star formation rates that are lower than predicted for the main sequence of star formation. (II) The distribution of impact parameter with Hi column density and with absorption-metallicity for absorbing galaxies at $z\sim 2-3$ extends to $z\sim 0.7$ and to lower Hi column densities. (III) A robust mean metallicity gradient of $\langle \Gamma \rangle = 0.022 \pm 0.001~[dex~kpc^{-1}]$. (IV) By correcting absorption metallicities for $\langle \Gamma \rangle$ and imposing a truncation-radius at $12~\mathrm{kpc}$, absorbing galaxies fall on top of predicted mass-metallicity relations, with a statistically significant decrease in scatter., Comment: 20 pages, 7 figures, accepted for publication in A&A 03/07/2018

We here apply a novel technique selecting quasar candidates purely as sources with zero proper motions in the Gaia data release 2 (DR2). We demonstrate that this approach is highly efficient toward high Galactic latitudes with < 25% contamination from stellar sources. Such a selection technique offers a very pure sample completeness, since all cosmological point sources are selected regardless of their intrinsic spectral properties within the limiting magnitude of Gaia. We carry out a pilot-study by defining a sample compiled by including all Gaia-DR2 sources within one degree of the North Galactic Pole (NGP) selected to have proper motions consistent with zero within 2-sigma uncertainty. By cross-matching the sample to the optical Sloan Digital Sky Survey (SDSS) and the mid-infrared AllWISE photometric catalogues we investigate the colours of each of our sources. Together with already spectroscopically confirmed quasars we are therefore able to determine the efficiency of our selection. The majority of the zero proper motion sources have optical to mid-infrared colours consistent with known quasars. The remaining population may be contaminating stellar sources, but some may also be quasars with colours similar to stars. Spectroscopic follow-up of the zero proper motion sources is needed to unveil such a hitherto hidden quasar population. This approach has the potential to allow substantial progress on many important questions concerning quasars such as determining the fraction of dust-obscured quasars, the fraction of broad absorption line (BAL) quasars, and the metallicity distribution of damped Lyman-$\alpha$ absorbers. The technique could also potentially reveal new types of quasars or even new classes of cosmological point sources., Comment: Submitted to A&A, comments welcome

Using the Atacama Large Millimeter/sub-millimeter Array, we have detected CO(3-2) line and far-infrared continuum emission from a galaxy associated with a high-metallicity ([M/H] = -0.27) damped Ly-alpha absorber (DLA) at z =2.19289. The galaxy is located 3.5" away from the quasar sightline, corresponding to a large impact parameter of 30 kpc at the DLA redshift. We use archival Very Large Telescope-SINFONI data to detect Halpha emission from the associated galaxy, and find that the object is dusty, with a dust-corrected star formation rate of 110 +60 -30 Msun/yr. The galaxy's molecular mass is large, Mmol = (1.4 +- 0.2) x 10^11 x (\alpha_CO/4.3) x (0.57/r_31) Msun, supporting the hypothesis that high-metallicity DLAs arise predominantly near massive galaxies. The excellent agreement in redshift between the CO(3-2) line emission and low-ion metal absorption (~40 km/s) disfavors scenarios whereby the gas probed by the DLA shows bulk motion around the galaxy. We use Giant Metrewave Radio Telescope HI 21cm absorption spectroscopy to find that the HI along the DLA sightline must be warm, with a stringent lower limit on the spin temperature of T_s > 1895 x (f/0.93) K. The detection of CI absorption in the DLA, however, also indicates the presence of cold neutral gas. To reconcile these results requires that the cold components in the DLA contribute little to the HI column density, yet contain roughly 50% of the metals of the absorber, underlining the complex multi-phase nature of the gas surrounding high-z galaxies., Comment: 6 pages, 3 figures, accepted for publication in ApJL

In vertebrates, offspring have a relatively inefficient immune system soon after birth. Female birds transmit immunoglobulins to the egg, which can confer protection against parasites to their offspring after hatching, but allocation of immune factors can depend on the reproductive value of the offspring as affected, for example, by the quality of their father. We analyse the variation in immunoglobulin levels of female barn swallows (Hirundo rustica) during the breeding cycle in relation to the expression of a secondary sexual character of their mates. Circulating immunoglobulins peaked on approximately the day before that of laying of their first egg, but postlaying concentration was similar to the concentration well before laying. Immunoglobulin levels per unit volume of plasma were lower for females breeding late compared with those breeding early. Haematocrit of females reached an absolute minimum on the day of laying of the third egg. In males, concentration of immunoglobulins relative to other plasma proteins did not change in relation to the breeding stage. Smaller relative concentrations of immunoglobulins and haematocrit were observed in males breeding late in the season. Immunoglobulin concentration of females was positively correlated with the level of ornamentation of their mates. These results suggest that females alter their immune profile to transmit humoral factors providing immune defence against pathogens to their offspring after hatching. This enhancement of immune protection is larger when the offsprings are of relatively large reproductive value as when sired by high quality males. Alternatively, females with relatively large immunoglobulin concentration preferentially mate with the most ornamented males.

Behavioral responses to environmental change are the mechanisms that allow for rapid phenotypic change preventing temporary or permanent damage and hence preventing reductions in fitness. Extreme climatic events are by definition rare, although they are predicted to increase in amplitude and frequency in the coming years. However, our current knowledge about behavioral responses to such extreme events is scarce. Here I analyze two examples of the effects of extreme weather events on behavior and life history: (1) A comparison of behavior and life history during extremely warm and extremely cold years relative to normal years; and (2) a comparison of behavior before and after the extremely early snowfall in fall 1974 when numerous birds died in the Alps during September-October. Behavioral and life history responses of barn swallows Hirundo rustica to extremely cold and extremely warm years were positively correlated, with particularly large effect sizes in cold years. Extreme mortality in barn swallows during fall migration 1974 in the Alps eliminated more than 40% of the breeding population across large areas in Central and Northern Europe, and this affected first arrival date, changes in timing and extent of reproduction and changes in degree of breeding sociality supposedly as a consequence of correlated responses to selection. Finally, I provide directions for research that will allow us to better understand behavior and life history changes in response to extreme climate change [Current Zoology 57 (3): 351–362, 2011].

The brood parasitic common cuckoo Cuculus canorus consists of gentes, which typically parasitize only a single host species whose eggs they often mimic. Where multiple cuckoo gentes co-exist in sympatry, we may expect variable but generally poorer mimicry because of host switches or inter-gens gene flow via males if these also contribute to egg phenotypes. Here, we investigated egg trait differentiation and mimicry in three cuckoo gentes parasitizing great reed warblers Acrocephalus arundinaceus, marsh warblers Acrocephalus palustris and corn buntings Miliaria calandra breeding in close sympatry in partially overlapping habitat types. The three cuckoo gentes showed a remarkable degree of mimicry to their three host species in some but not all egg features, including egg size, a hitherto largely ignored feature of egg mimicry. Egg phenotype matching for both background and spot colours as well as for egg size has been maintained in close sympatry despite the possibility for gene flow.

Senescence arises from age-specific deterioration of the soma as a consequence of optimization of life history, and such effects of senescence should appear when comparing species that differ in intensity of sexual selection, as well as when comparing, within a species, the two sexes that often differ in intensity of sexual selection. However, any extrinsic cause of mortality that reduces life expectancy will reduce the possibility of detecting sex-specific differences in senescence. We investigated geographical variation in human sex differences in longevity across 121 countries to test whether differences in sexual competition for limiting resources, reflecting intensity of sexual selection, affected sex differences in longevity. Men on average lived 5 years shorter than women. High rates of childhood morbidity and mortality reduced the sex difference in longevity, while increased overall longevity increased the sex difference in longevity. Increased resource availability estimated from gross domestic product per capita reduced the sex difference in longevity, accounting for 10% of the variance, while there was no additional effect of income inequality as reflected by the Gini coefficient. In a separate analysis of sex differences in longevity among the states of the US, there was a strong effect of the Gini coefficient on sex difference in longevity, with the negative effect on male longevity being stronger than that on female longevity. In contrast, there was only a marginal effect of average household income. Thus, there was evidence of increased competition for resources contributing to increased sex differences in longevity within a single nation.

Climate change has advanced the phenology of many organisms. Migratory animals face particular problems because climate change in the breeding and the wintering range may be asynchronous, preventing rapid response to changing conditions. Advancement in timing of spring migration may have carry-over effects to other parts of the annual cycle, simply because advancement of one event in the annual cycle also advances subsequent events, gradually causing a general shift in the timing of the entire annual cycle. Such a phenotypic shift could generate accumulating effects over the years for individuals, but also across generations. Here we test this novel hypothesis of phenotypic response to climate change by using long-term data on the Arctic tern Sterna paradisaea. Mean breeding date advanced by almost three weeks during the last 70 years. Annual arrival date at the breeding grounds during a period of 47 years was predicted by environmental conditions in the winter quarters in the Southern Ocean near the Antarctic and by mean breeding date the previous year. Annual mean breeding date was only marginally determined by timing of arrival the current year, but to a larger extent by arrival date and breeding date the previous year. Learning affected arrival date as shown by a positive correlation between arrival date in year (i + 1) relative to breeding date in year (i) and the selective advantage of early breeding in year (i). This provides a mechanism for changes in arrival date being adjusted to changing environmental conditions. This study suggests that adaptation to changing climatic conditions can be achieved through learning from year to year

Objective The long-term results are presented on total survival, cancer-specific survival and recurrence in 143 consecutive patients treated with transanal endoscopic microsurgery (TEM) for adenocarcinoma of the rectum. Method Four Danish centres established in 1995 a database for registration of all TEM procedures. Data were supplemented from pathology reports and death certificates were checked in the Danish patient registry. Data were analysed with multivariance regression and survival analysis. Results The T stage was as follows: T1 50%, T2 33%, T3 14%, and stage unknown 3%. TEM was performed with curative intent in 43%, for compromise in 52% and for palliation in 5%. Five-year total survival was 66% and 5-year cancer-specific survival 87%. Cancer-specific survival for T1 was 94%. The significant predictors for total survival were age and tumour size. For cancer-specific survival T stage, radical resection, tumour size and recurrence were significant predictors. Eighteen per cent had recurrence and 15% had immediate reoperation. Conclusion The TEM provides good long-term results for pT1 cancers. In old patients and patients with co-morbidity TEM may provide acceptable long-term results for T2 cancers. Tumours larger than 3 cm should not be treated with TEM for cure.

At the center for Rehabilitation of Brain Damage, University of Copenhagen, 46 consecutively admitted brain-damaged patients with varying pathologies and who were on average 2.9 years post-injury were treated in a daily four-month rehabilitation program in groups of about 10, followed by a six-month period of contact varying according to individual needs. An evaluation of psychosocial outcome is presented. The results, based on comparisons between pre-, post-treatment and follow-up questionnaire data, show continuing functional improvements in the areas of family life and living conditions. Dependence on health services declined. Over 70% of the patients returned to either work, further education or voluntary work activities. For the whole group, leisure activities returned to the pre-injury level. Follow-up at about two years revealed continuing improvements in all areas, suggesting social readaptation to a degree above expectations as judged from the existing literature

Of 99 consecutive patients with meningococcal disease, 6 died during the acute stage. The 93 survivors were examined one year after hospitalization. 21 (40%) of the adults and 6 (15%) of the children had definite sequelae, and an additional 27% and 11% possible sequelae. 6 adults (12%) and 1 child (2%) had definite neurological sequelae. Electroencephalography (EEG) abnormalities were observed in 7 adults (14%) and 2 children (5%). Epileptogenic activity was present in 3 of these, but none had experienced seizures. 8 adults (19%) and 5 children (14%) had sensorineural hearing loss or impaired vestibular function. Cerebral computerized tomography (CT) scan showed definite and possible abnormalities in 1 (3%) and 6 (18%), respectively, of the 34 patients tested. Neuropsychological tests were performed in 9 patients, 2 of these showed definite impairment. The frequency of neurological abnormalities was higher than in many previous studies, probably reflecting the more comprehensive examinations performed in the present study. However, only 3 patients had serious sequelae. The results suggest that the occurrence of sequelae after meningococcal disease is related to the severity of the acute disease. This may explain the higher rate of sequelae in adults, who have a higher proportion of seriously ill patients. The presence of meningitis is not required for the occurrence of neurological sequelae.

We investigated the motility and morphology of live sperm from barn swallows Hirundo rustica breeding in radioactively contaminated areas around Chernobyl and control areas in Ukraine in order to test the hypothesis that swimming behaviour and morphology of sperm was impaired by radioactive contamination. We obtained sperm samples from 98% of sampled birds, thus avoiding sampling bias due to the fraction of males not producing sperm samples. Analyses of within- and between-sample repeatability revealed significant and intermediate to large estimates for all sperm parameters. There were significant differences between the Chernobyl area and the control area for two of 11 sperm behaviour parameters, and significant interactions between area and year for six of these parameters. The proportion of sperm with abnormal morphology was elevated in barn swallows from Chernobyl. A principal component (PC) analysis revealed four significant axes that explained 88% of the variance in sperm behaviour parameters. One of these principal components differed between areas, and three components showed significant year by area interactions. PC2 representing the frequency of slow sperm increased with increasing radiation in one year, but not another. PC3 representing sperm with high linearity, small amplitude of lateral head displacement and low track velocity decreased with increasing background radiation level. PC4 reflecting a large proportion of static sperm with high beat cross frequency increased with increasing background radiation level. Sperm behaviour as reflected by principal components was predictable among years from information on level of radiation, and it was predictable among sites in different years. These results are consistent with the hypothesis that sperm behaviour and morphology have been affected by radiation due to the Chernobyl accident.

The composition of the gut microbiota and excessive ingestion of high-fat diets (HFD) are considered to be important factors for development of obesity. In this review we describe a coherent mechanism of action for the development of obesity, which involves the composition of gut microbiota, HFD, low-grade inflammation, expression of fat translocase and scavenger receptor CD36, and the scavenger receptor class B type 1 (SR-BI). SR-BI binds to both lipids and lipopolysaccharide (LPS) from Gram-negative bacteria, which may promote incorporation of LPS in chylomicrons (CMs). These CMs are transported via lymph to the circulation, where LPS is transferred to other lipoproteins by translocases, preferentially to HDL. LPS increases the SR-BI binding, transcytosis of lipoproteins over the endothelial barrier,and endocytosis in adipocytes. Especially large size adipocytes with high metabolic activity absorb LPS-rich lipoproteins. In addition, macrophages in adipose tissue internalize LPS-lipoproteins. This may contribute to the polarization from M2 to M1 phenotype, which is a consequence of increased LPS delivery into the tissue during hypertrophy. In conclusion, evidence suggests that LPS is involved in the development of obesity as a direct targeting molecule for lipid delivery and storage in adipose tissue.

The evolution of lifespan is a central question in evolutionary biology, begging the question why there is so large variation among taxa. Specifically, a central quest is to unravel proximate causes of ageing. Here, we show that the degree of unsaturation of liver fatty acids predicts maximum lifespan in 107 bird species. In these birds, the degree of fatty acid unsaturation is positively related to maximum lifespan across species. This is due to a positive effect of monounsaturated fatty acid content, while polyunsaturated fatty acid content negatively correlates with maximum lifespan. Furthermore, fatty acid chain length unsuspectedly increases with maximum lifespan independently of degree of unsaturation. These findings tune theories on the proximate causes of ageing while providing evidence that the evolution of lifespan in birds occurs in association with fatty acid profiles. This suggests that studies of proximate and ultimate questions may facilitate our understanding of these central evolutionary questions.

Costs and benefits are ill-defined terms; this review discusses some interpretations and some ways of assessing costs and benefits. Cost-benefit calculation is a misconception in health care, in that costs should be considered together with what the patients gain in terms of prolonged life and life quality. In the emerging commercialization of Western health care, however, money benefit is to be calculated in order to ask for funding. As professionals we are dealing with inherited breast cancer. We come to know the families and the individual women within these families. We care, we cure, we prevent and we are gaining knowledge. We are doing good, not harm. We are profoundly involved, and we are so deeply and personally involved that we may not even try to be objective in a debate on choosing between our patients and patients cared for by others. As we present our arguments, society has to decide. There is no argument to provide service for other inherited disorders based on ethical standards alone, and to deprive women at risk for breast cancer the life-saving health service they need because of money costs. The money needed is based on a number of rapidly fluctuating factors: the most stable costs are genetic counseling and physical examinations to demonstrate disease, while laboratory costs are rapidly decreasing. The overall conclusion is that economical cost-benefit analysis as an argument for our activity is outside our professional ethics, and should never become a reason for our continued endeavors.

Regulated and selective transport of biomolecules across biological membranes is mediated by transporter proteins. These are essential in physiological processes ranging from electrical signaling in the nervous system to secretion of hormones and maintenance of electrochemical gradients. Though transporters are extensively studied, there are currently no techniques available to investigate their function at the single molecule level. We have developed a fluorescence-based assay to monitor thousands of individual nanoscale proteoliposomes in parallel by immobilizing them on functionalized glass surfaces (1-5).We recently extended this assay to allow real-time observation of substrate translocation mediated by single transporters reconstituted into liposomes. Here we present the development of the assay including characterization and optimization of the most critical system components including: minimization of leakage, in situ real-time calibration of absolute transport rates and increase of photostability for long term recordings (∼40min). We also present high-resolution single molecule activity recordings of the transporter protein, Arabidopsis thaliana H+-ATPase (AHA2).References:1. Mathiasen et al., Nature Methods. (2014) 11:931-342. Christensen. et al. Nat Nanotechnol. (2012) 7:51-53. Hatzakis. et al. Nat. Chem. Biol. (2009) 5:835-414. Bendix. et al. PNAS. (2009) 106:12341-65. Kunding. et al. Biophys J. (2008) 95:1176-88

Trauma resuscitation is a complex situation, and most organisations have multi-professional trauma teams. Non-technical skills are challenged during trauma resuscitation, and they play an important role in the prevention of critical incidents. Simulation-based training of these is recommended. Our research question was: Does simulation-based trauma team training of non-technical skills have effect on reaction, learning, behaviour or patient outcome? The authors searched PubMed, EMBASE and the Cochrane Library and found 13 studies eligible for analysis. We described and compared the educational interventions and the evaluations of effect according to the four Kirkpatrick levels: reaction, learning (knowledge, skills, attitudes), behaviour (in a clinical setting) and patient outcome. No studies were randomised, controlled and blinded, resulting in a moderate to high risk of bias. The multi-professional trauma teams had positive reactions to simulation-based training of non-technical skills. Knowledge and skills improved in all studies evaluating the effect on learning. Three studies found improvements in team performance (behaviour) in the clinical setting. One of these found difficulties in maintaining these skills. Two studies evaluated on patient outcome, of which none showed improvements in mortality, complication rate or duration of hospitalisation. A significant effect on learning was found after simulation-based training of the multi-professional trauma team in non-technical skills. Three studies demonstrated significantly increased clinical team performance. No effect on patient outcome was found. All studies had a moderate to high risk of bias. More comprehensive randomised studies are needed to evaluate the effect on patient outcome.

Duration of untreated psychosis (DUP) might affect the course and treatment outcome in schizophrenia significantly. Looking for potential determinants of DUP, researchers have mostly assessed the initial untreated psychosis in terms of plain duration, not developmental content. The present study provides empirical contributions to the discussion of phenomenal continuity throughout the entire untreated illness by pointing out continuous trajectories from prodrome to psychosis. In a series of 19 first-episode DSM-IV schizophrenia patients, we explore prodromal and untreated psychotic phenomena, and the data indicate that symptoms considered intrinsic to core psychosis phenomenology had attenuated antecedents in the prodromes, increasing continuously until treatment, whereas symptoms considered not intrinsic to core psychosis were characterized by fluctuations. Mode of initial psychosis development is proposed as a tentatively informative concept, comprising duration and content aspects of the initial prodrome and untreated psychosis.

The clinical, histological, phenotypic and genotypic features of 21 primary cutaneous B-cell lymphomas (CBCLs) have been investigated. The patients were 13 men and eight women aged 34-91 years (median 67) at diagnosis. Eighteen patients had localized disease, and three had multiple skin lesions at diagnosis. Twelve patients developed cutaneous or extracutaneous recurrences, and five died from malignant lymphoma 7-84 months (median 36) after diagnosis. Histological examination showed features of marginal zone/mucosa-associated lymphoid tissue (MALT)-type lymphoma in 12 cases. Three of these had transformed to diffuse large B-cell lymphoma (DLBCL) in relapse biopsies. The remaining cases were seven primary DLBCLs and two cases tentatively classified as follicle centre cell (FCC) lymphoma. The neoplastic B cells showed similar phenotypes and genotypes in most cases (CD20+, CD79+, CD5-, CD10-, cyclin D1-, bcl-2+, bcl-x-, bax-, t(14;18)-negative). p53 protein was expressed in five cases, and four harboured mis-sense or loss-of-function mutations in the p5 3 gene. Deletion or promoter region hypermethylation of the p16 INK4a gene was detected in two patients with DLBCL, The level of retinoblastoma protein expression and the proliferative fraction were significantly higher in DLBCL (> 50%) than in MALT- or FCC-type lymphomas (< 10%). Features associated with an unfavourable prognosis were the presence of multiple skin lesions at diagnosis, transformation from MALT-type lymphoma to DLBCL, and possibly p16 INK4a aberrations. It is concluded that most CBCLs are dissimilar from FCC lymphomas and seem to be more closely related to marginal zone/ MALT-type lymphomas. It is also suggested that there are fundamental differences between DLBCL and other histological categories of CBCL, indicating that cutaneous DLBCL is a separate entity with an increased growth potential and genetic features similar to DLBCL originating in other anatomical sites.

We present measurements of the Fundamental Plane (FP) parameters (the effective radius, the mean effective surface brightness, and the central velocity dispersion) of six field elliptical galaxies at intermediate redshift. The imaging is taken from the Medium Deep Survey of the Hubble Space Telescope, while the kinematical data are obtained from long-slit spectroscopy using the 3.6-m ESO telescope. The Fundamental Plane appears well defined in the field even at redshift $\approx$ 0.3. The data show a shift in the FP zero point with respect to the local relation, possibly indicating modest evolution, consistent with the result found for intermediate redshift cluster samples. The FP slopes derived for our field data, plus other cluster ellipticals at intermediate redshift taken from the literature, differ from the local ones, but are still consistent with the interpretation of the FP as a result of homology, of the virial theorem and of the existence of a relation between luminosity and mass, $L \propto M^{\eta}$. We also derive the surface brightness vs. effective radius relation for nine galaxies with redshift up to $z \approx0.6$, and data from the literature; the evolution that can be inferred is consistent with what is found using the FP., Comment: 17 pages, including 9 figures, MNRAS, accepted

Adsorption and decomposition of HCOOH on potassium-modified Cu(110) surface has been investigated by temperature-programmed desorption (TPD) and photoemission. It has been found that preadsorbed potassium enhances deprotonation of HCOOH at low temperatures. Potassium, in coverages up to one monolayer, stabilizes a formate species at 300–450 K characterized by photoemission peaks at 5.8, 9.0–9.3, 10.9 and 14.3 eV in the HeII spectrum. The tendency to create potassium carbonate species has been found at high temperatures for potassium coverages greater than one monolayer.

Mantle cell lymphomas (MCL) are morphologically and immunophenotypically distinctive lymphoid neoplasms characterised by overexpression of cyclin D1. Recent studies have suggested that co-operating aberrations of cell cycle associated genes may provide a growth advantage to a tumour. To address this issue further, we investigated five typical and three aggressive (blastoid) MCL for alterations in the cell cycle regulating genes p15, p16, CDK4, Rb and p53. In 3/3 aggressive cases with cyclin D1 overexpression we found aberration of at least one additional gene. One case showed diminished expression of the retinoblastoma protein (pRb); one case harboured deletion of both p15 and p16; and one case exhibited both deletion of p16 and point mutation of p53. However, we also identified two typical cases which in addition to cyclin D1 overexpression exhibited diminished pRb expression and p15 and p16 hypermethylation, respectively. Our findings confirm and extend other recent investigations and indicate that co-operating genetic alterations of cell cycle-associated genes may contribute to the pathogenesis of MCL.