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Background: It is well-established that microRNAs (miRNAs) have regulatory roles in the fibrotic processes of various tissues. Studies revealed that some miRNAs play a pivotal role in the development of idiopathic pulmonary fibrosis (IPF). Methods: In this single-center, cross-sectional study, the plasma levels of miR-21, miR-590, miR-192, and miR-215 in IPF (n=88) and the control (n=20) group were investigated using real-time PCR. The pulmonary function tests and the plasma sampling of participants were carried out simultaneously. Patients were grouped according to age, forced vital capacity, diffusing capacity for carbon monoxide (DLCO), and the Gender-Age-pulmonary Physiology (GAP) score. The expression levels of the target miRNAs between these clinical subgroups were compared.Results: The IPF and control groups were similar in terms of gender and age. The mean plasma miR-21 and miR-590 levels in IPF were significantly higher than in the control (pConclusions: Our results suggest that higher plasma levels of miR-21 and miR-590 were related to the presence of IPF. While all target miRNAs correlated with age, the miR-21 and miR-590 were associated with DLCO, and miR-192 and miR-215 were associated with GAP score and honeycombing.

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BackgroundChromosomal microarray analysis is the first-tier test for the evaluation of developmental disabilities and congenital anomalies. In this report, we present CMA results of 971 patient and 301 parent samples.

WOS: 000425238000040, The purpose of this study is to examine emotional labor levels of teachers and the emotional labor dimensions of the scale with regards to the demographic characteristics of the participants. With this respect, the Emotional Labor Scale, developed by Diefendorff, Croyle, and Gosserand (2005) and adapted into Turkish by Basim and Begenirbas (2012), was used in the study. The study sample consists of a total of 277 teachers from 21 schools selected through the random sampling method. According to the data analysis results, teachers avoided role making and behaved as required by their profession. A significant difference was detected in favor of female teachers only for the scale's genuine emotions dimension with regards to the gender variable. According to the Kruskall Wallis-H test results, which was conducted according to the branch variable, with regards to the items of the deep role making dimension, there is a significant difference between classroom teachers and the science/math branch teachers in favor of classroom teachers and between science/math teachers and social science teachers in favor of the teachers from the social science branch. According to the Kruskall Wallis-H test results concerning the seniority variable, there is a significant difference only between the items of the genuine emotions dimension. Analysis results concerning the institution type variable show that as the age group of the students increase, the interaction created with students becomes more superficial.

WOS: 000378612600018, The purpose of this study is to determine the perceptions of educational employees regarding change with regards to various variables. The study was carried out with a qualitative design. An interview form compatible with the study aims was created and content and face validity of the form was determined by consulting with expert opinion. Research findings suggest that change is required in schools and that changes should begin with upper management. In addition, because of a lack of information in educational organizations and because employees were not motivated by their managers regarding change, a resistance against change was detected. When viewed from this perspective, in order to cope with this resistance and promote change successfully, employees are required to agree with the decision by way of making an effective plan.

WOS: 000373422500016, The purpose of this study is to examine the effectiveness of mentoring relationship between faculty members and post-graduate students according to various variables. The sample consists of 99 post-graduate students studying at public universities in Turkey. The Mentoring Effectiveness Scale, developed by Berk, Berg, Mortimer, Walton-Moss, and Yeo (2005) and adapted into Turkish by Yirci, Karakose, Uygun, and Ozdemir (2016) was used as the data collection instrument. According to the study findings, post-graduate students in general find the effectiveness levels of mentoring relationships in universities sufficient. Accordingly, it is suggested that the frequency of mentor and mentee meetings should increase. While the effectiveness of mentoring relationship is not affected by age and gender variables; it is influenced by the frequency of meetings and the meeting method preferred. Study findings also suggest that mentees expect to be appreciated more than the mentors.

PubMed ID: 24772975, The endoplasmic reticulum (ER) is related to the various signal routes that are activated in unfolded protein response (UPR). The Grp78, Grp94, CHOP, MTJ1 and HMOX1 genes expressions demonstrate UPR activity. In this study, we investigated the UPR gene expressions in larynx epidermoid carcinoma (HEp2) to which dexamethasone (dex) was applied. HEp2 cells were administered for 48 h with different combinations using 0.1 µM and 1 µM dex, 1 mM phenyl butyric acid (PBA) and 100 ng/ml lipopolysaccharide (LPS). The Grp78, Grp94, CHOP, MTJ1 and HMOX1 genes expression was determined using quantitative RT-PCR. The Grp78, MTJ1 and HMOX1 gene expression increased with the administration of 1 µM dex. CHOP expression, on the other hand, decreased with 0.1 µM dex. When dex was combined with LPS, nearly all gene expressions decreased. The increase in Grp78, Grp94, HMOX1 and MTJ1 gene expression was greater in groups in which dex was administered in combination with PBA than in groups in which dex was administered alone. Dex in low dose (0.1 µM) caused a decrease in CHOP expression in HEp2 cells and an increase in Grp78 expression, in particular. The changes in UPR genes expressions may lead to the extended survival of the cells.

WOS: 000341974800007 PubMed: 24943970 BACKGROUND: 1,1-dimethylbiguanide hydrochloride (biguanide metformin) is a hypoglycemic agent that is widely used in the treatment of Type 2 diabetes. Use of metformin was found to be associated with the lower risk of cancer. It is suggested that metformin has an anticancer and antiproliferative effect and affects the apoptosis by activating the AMPK and inhibiting the mammalian target of rapamycin (mTOR). Although the effects of metformin treatment of various types of cancers are defined with many mechanisms, the literature provides only sufficient information about how it affects the SGK-1, Par-4 and Cav-1 mRNA expressions and the impact of this effect on cytotoxicity. The breast cancer is globally one of the most important causes of cancer-related mortality for women. We, therefore investigated the possible effects of metmorfin on proliferation, cytotoxicity and some unfolded protein response (UPR) genes in the breast cancer cells (MCF-7). MATERIALS AND METHODS: We administrated 0.31 mM, 2.5 mM and 10 mM of metformin alone and in combination with 2-DG to the MCF-7 cells and monitored the cell viability and proliferation with real-time cell analyzer system for 48 hours. We also measured CHOP, Cav-1, HO-1, SGK-1 and Par-4 genes mRNA expression levels using Real Time-Polymerase Chain Reaction (RTPCR). We considered the GAPDH gene as reference gene and the control groups as calibrator. We performed an analysis for relative gene expressions of the study groups. RESULTS: Metformin caused transcriptional regulation of UPR and tumor-related genes in MCF-7 cells and inhibited the proliferation depending on the dose, resulting in cytotoxic effect. CONCLUSIONS: We consider that administration of metformin with chemotherapeutic agents could be an effective method in treatment of breast cancer through mechanisms such as reduced resistance to chemotherapy and increased cytotoxic activity.

Two new dibenzosuberane-substituted fullerene derivatives, dibenzosuberane-C60 monoadduct (DBSCMA) and bis-adduct (DBSCBA) were synthesized using a classical cyclopropanation reaction via a tosylhydrazone route for application as acceptor materials in polymer solar cells (PSCs). DBSCBA shows good solubility in common organic solvents and both derivatives were characterized by 1HNMR, 13C NMR, MALD-TOF, elemental analysis and UV-vis absorption measurements. The shift of fullerene energy levels induced by the dibenzosuberane substitution was investigated by using theoretical simulations and ultraviolet photoelectron spectroscopy. Bulk-heterojunction PSCs based on poly (3-hexylthiophene) (P3HT) and dibenzosuberane-C60 derivatives were fabricated and optimized by adjusting the donor/acceptor ratio and using thermal annealing and solvent additive. The morphologies of the active layers processed under different conditions were also examined by atomic force microscopy. When tested under an illumination of AM 1.5 G at 100 mW/ cm2, the highest power conversion efficiency of the devices using DBSCBA is 3.70% which is superior to that of conventional P3HT:PCBM devices. © 2013 Elsevier B.V.

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13th Congress of the European-Hematology-Association -- JUN 12-15, 2008 -- Copenhagen, DENMARK WOS: 000263430201518 … European Hematol Assoc

WOS: 000230331700005 Fourteen different moss samples were used as bioindicators to study the atmospheric deposition of metals around polluted and unpolluted areas in Trabzon, Turkey. Samples were analyzed using flame and graphite furnace atomic absorption spectrometry (AAS) after microwave digestion. Good accuracy was assured by the analysis of standard reference material. A total of six trace metals (Fe, Cu, Mn, Zn, Pb, Cd) were determined. Metal concentrations were in the mu g/g range 1150.0-6137 (Fe), 13.6-33.6 (Cu), 329.2-806.0 (Mn), 84.4-144.4 (Zn), 9.230.4 (Pb), and 0.7-1.7 (Cd).

PubMed ID: 22757644, Ouabain is a cardiotonic steroid and specific inhibitor of the Na +/K+-ATPase. The relationship between ouabain treatment and the unfolded protein response (UPR) in cells is not precisely understood. Therefore, we studied the possible effects of ouabain on proliferation, apoptosis, and the UPR. HepG2 cells were cultured overnight and then treated with various concentrations of ouabain (0.75 to 750nM) in the absence or presence of 10mM 2-deoxyglucose (2-DG) for 48 hours. We also used real-time polymerase chain reaction to obtain quantitative measurements of expression levels of Grp78, Grp94, CHOP, MTJ-1, HKII, MDR-1, MRP-1, HO-1, and Par-4. Cell number, viability, and proliferation of HepG2 cells were monitored with a real-time cell analyzer system (xCELLigence). We show that ouabain modulates the UPR transcription program and induces cell death in glucose-deprived tumor cells. Ouabain at all concentrations showed no cytotoxicity whereas all concentrations were very effective under 2-DG stress conditions. Our findings show that disruption of the UPR during glucose deprivation could be an attractive approach for selective cancer cell killing and could provide a chemical basis for developing UPR-targeting drugs against solid tumors. Ouabain use as an adjunct to conventional cancer therapy also warrants vigorous investigation. © Copyright 2012, Mary Ann Liebert, Inc. 2012.

The self-assembly of nanoparticles into higher organizations in a controlled manner has critical importance for the utility of the unique properties of nanoparticles. The behavior of magnetic Fe3O 4 nanoparticles (MNPs) with an average size of 6nm under an enhanced magnetic force is reported. Upon evaporation of the solvent where the MNPs are suspended, formation of unique micrometer-sized structures is achieved only when there is a patterned surface constructed from sub-micrometer size magnetic beads in between the applied magnetic field and the MNPs. The preliminary results indicate that the combined effect of magnetic field and evaporation rate might help the control of nanoparticle behavior on surfaces and interfaces in constructing higher structures. © 2010 IOP Publishing Ltd.