1. Clinopodium nubigenum (Kunth) Kuntze essential oil: Chemical composition, antioxidant activity, and antimicrobial test against respiratory pathogens
- Author
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Antonio Osorio Edison, Fonseca Andrea, Guerra Pablo, Fernando Noriega Paco, and de Los Ángeles Mosquera Tatiana
- Subjects
0301 basic medicine ,ABTS ,biology ,Chemistry ,DPPH ,030106 microbiology ,Thymus vulgaris ,biology.organism_classification ,law.invention ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,law ,Drug Discovery ,Carvacrol ,Food science ,Butylated hydroxyanisole ,Pulegone ,Thymol ,Essential oil - Abstract
The essential oil of leaves and flowers of Clinopodium nubigenum (Kunth.) Kuntze (Lamiaceae) collected in the province of Pichincha-Ecuador was steam distilled and analyzed by gas chromatography mass spectrometry (GC/MS) to determine its chemical composition. The majority of the compounds identified were carvacrol acetate (42.1%), carvacrol (20.6%), pulegone (6.3%) and thymol (5.5%). Antioxidant activity was assessed by the assays of diphenylpicrylhydrazyl (DPPH) (IC50: 1.8 μl / ml), 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) (IC50: 0.3 μl / ml) and β-carotene (IC50: 0.031 μl / ml) compared to Thymus vulgaris and butylated hydroxyanisole (BHA) as referents. The specie also shows a promising medicinal potential exhibiting significant antibacterial activity at different concentrations against Staphylococcus aureus (2.5% v/v), Streptococcus pyogenes (0.6% v/v), Streptococcus pneumoniae (0.6% v/v) and Streptococcus mutans (0.6% v/v), suggesting an interesting natural alternative in the fight against bacteria that generate resistance to other antibiotics. Key words: Clinopodium nubigenum, gas chromatography mass spectrometry (GC/MS), 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), diphenylpicrylhydrazyl (DPPH), antimicrobial test.
- Published
- 2018