1. Cutting edge: alloimmune responses against major and minor histocompatibility antigens: distinct division kinetics and requirement for CD28 costimulation
- Author
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Hk, Song, Noorchashm H, Yk, Lieu, Rostami S, Siri Atma W Greeley, Cf, Barker, and Naji A
- Subjects
CD4-Positive T-Lymphocytes ,Mice, Inbred C57BL ,Minor Histocompatibility Antigens ,Mice ,Mice, Inbred BALB C ,Mice, Inbred C3H ,CD28 Antigens ,Histocompatibility Antigens ,Animals - Abstract
Comparative study of alloimmune responses against major and minor histocompatibility Ags has been limited by the lack of suitable assays. Here, we use a bioassay that permits tracking of alloreactive CD4+ T cell populations as they proliferate in response to major or minor histocompatibility Ags in vivo. Division of alloreactive CD4+ T cells proceeded more rapidly in response to major histocompatibility Ags than minor Ags, although CD4+ T cells alloreactive to minor Ags had a similar capacity to divide successively up to eight times after stimulation. Allorecognition of minor histocompatibility Ags was highly dependent on CD28 costimulation, with the frequency of CD4+ T cells proliferating in response to minor Ags in the absence of CD28 costimulation reduced up to 20-fold. These findings highlight differences in signaling processes that lead to allorecognition of major and minor histocompatibility Ags and have implications on the design of interventions aimed at abrogating these responses.
- Published
- 1999